JP2015157770A - Cosmetics - Google Patents

Abstract

[PROBLEMS] To improve the epidermal barrier function to form a healthy epidermis and to suppress the aging of dermal fibroblasts, thereby forming a skin with elasticity and elasticity, which has no side effects and is excellent in safety. Provision of cosmetics and cosmetics. Furthermore, the provision of an active ingredient that can solve multiple skin problems with one ingredient at a time. A cell differentiation promoter comprising a fermented product obtained by fermenting the roots of yellow rapes such as Yakukan elephant, Honkan elephant, Zentica (Nikkoukigege), Hosobakisuge, Manshuukigege, etc., by lactic acid bacteria as an active ingredient Function improving agent, SA-β-Gal activity inhibitor, radical scavenger, and cosmetics. The lactic acid bacterium is preferably Lactobacillus plantarum. [Selection] Figure 1

Description

The present invention is a variety of agents containing as an active ingredient a fermented product obtained by fermenting the root part of yellow rape with lactic acid bacteria. Cell differentiation promoting effect, barrier function improving effect, cell aging inhibiting effect, SA-β-Gal activity inhibiting The present invention relates to a cosmetic containing a metabolite having a high DPPH radical scavenging activity effect as an active ingredient.

The yellow flower vegetables used in the present invention are those of the genus Forget-me-nots (Hemerocallis fulva var. Kwanso), Zentika (H. dumortieri var. L.), H.flava var. Minor, and these related plants. In the market, it is commonly known as “Yellow Vegetables”, which is dried “Yellow Vegetables” and is also called “Golden Needle”. In Chinese cuisine, it is reconstituted with water and eaten as a soup ingredient. Even when there is a description that does not specify the use site of the cosmetics using yellow rape, it is “bamboo” of “yellow rape” that has actually confirmed the effect.
Specifically, Patent Document 1 describes an external composition for skin having antioxidation, anti-inflammatory and whitening effects, Patent Document 2 describes a fibroblast activation action and a tyrosinase activity inhibitory action. Describes the slimming effect by accelerating the degradation of adipose tissue, Patent Document 4 describes the effect of preventing and improving swelling, and the effect of skin tightening, and Patent Document 5 describes the wrinkle improving action by suppressing protein glycation, dullness. The description of the improvement action is described in Patent Document 6, which describes the wrinkle prevention / improvement effect by normalizing or stabilizing the blood vessel due to the Tie2 activation effect. However, each of these effects describes an effect using an extract of a yellow rape plant itself.
On the other hand, Patent Document 7 obtains a degranulation inhibitory action, a prostaglandin E2 production inhibitory action, and a radical scavenging action by treating a plant belonging to the genus Forget-me-not with a proteolytic enzyme and then fermenting it with yeast. The example of the cosmetics characterized by mix | blending fermented material is described.

However, there are no examples of cosmetics characterized by blending fermented products obtained by fermenting forget-me-not plants with lactic acid bacteria. Yeasts are abundant in sap, nectar, fruits, etc. in the natural world, and since they decompose sugars to produce alcohol and carbon dioxide, they have long been used in various foods such as bread, beer, wine, whiskey, and miso. Has been used for manufacturing. On the other hand, since lactic acid bacteria decompose saccharides to produce lactic acid, fermenting vegetable materials such as vegetables, beans, rice, and wheat will change the taste and aroma of these materials, and the produced lactic acid will It has been used to improve the storage stability by suppressing the growth of microorganisms.

Thus, yeast and lactic acid bacteria are greatly different in components that are metabolized even if the same saccharide is decomposed. Therefore, even when a forget-me-not plant is fermented with a lactic acid bacterium, it is considered that the forget-me-not plant is greatly different from a substance produced by fermentation of a yeast.

JP 2006-052165 A JP 2006-143670 A JP2007-297372 JP2009-091317 JP2011-195530 JP 2011-201811 A JP2008-050326

The object of the present invention is to provide various agents having no side effects and excellent safety, thereby improving the epidermal barrier function to form a healthy epidermis and suppressing aging of dermal fibroblasts. -To provide cosmetics that can form elastic skin.
In addition, it is an object to provide an active ingredient that can solve a plurality of skin problems at once with one active ingredient.

In view of the problems of the prior art, the inventor of the present application has conducted extensive research on methods for solving these problems, and as a result, has the above-mentioned problem by having a fermented product obtained by fermenting the root of yellow rape with lactic acid bacteria. Has been found to be able to be solved, and the present invention has been completed.

The fermented product obtained by fermenting the root part of the lily family plant with lactic acid bacteria is stronger in promoting cell differentiation, improving the barrier function, and suppressing the cell aging than the extract of yellow flower rape before fermentation treatment. And SA-β-Gal activity inhibitory effect and radical scavenging action. However, the extract that has not been subjected to fermentation treatment has weak cell differentiation promoting effect, cell aging inhibitory effect, SA-β-Gal activity inhibitory effect, and DPPH radical scavenging action. It was thought that the ingredients changed and the effect was enhanced.

The evaluation criteria of cell differentiation are shown.

Hereinafter, the present invention will be described in detail.
First, an outline of the present invention will be described.
It is roughly divided into the following steps.
(1st process) The process of obtaining the leaching liquid which leached the root part of yellow flower vegetables.
(Second step) A step of inoculating a lactic acid bacterium previously cultured in the leachate obtained in the first step and culturing it under a certain condition to obtain a fermented product.
(Third step) A step of dividing the fermented product obtained in the second step into a fermentation broth and a residue by filtration or the like.
(4th process) The process of adding a solvent etc. to the residue obtained at the 3rd process, and obtaining a fermentation extract.
In this application, the effective substance obtained by the 3rd process and the 4th process is utilized.

The terms in the present invention are defined as follows.
(1) The leaching solution refers to a product obtained by leaching the root part of yellow rapeseed used in the present application with a solvent such as purified water or ethanol, and refers to a stage before the root part of yellow rapeseed is removed by filtration or the like. So-called plant extracts that are appropriately heated are also included.
(2) The pre-culture extract refers to an extract obtained by removing the roots of yellow rape from the leachate by filtration or the like.
(3) The fermented product refers to a product obtained by inoculating a leachate with lactic acid bacteria or the like and culturing for a certain period.
(4) The fermentation broth refers to a product obtained by removing the root portion fermented by filtration or the like from the fermented product.
(5) The fermented extract refers to a product obtained by adding an extraction solvent such as purified water to the fermented root part removed in (4), extracting under certain conditions, and then removing the residue by filtration or the like. .
(6) The fermented product is a general term that combines a fermentation broth and a fermentation extract.
In the present application, a comparative experiment is performed when the lactic acid bacterium is treated later and the yeast is treated. However, in order to distinguish between the two, the “fermented product” is referred to as the “lactic acid bacterium fermented product” and the “fermentation culture solution”. Are sometimes synonymous with “lactic acid bacteria fermentation broth” and “fermentation extract” as “lactic acid bacteria fermentation extract”.
In addition, when performing the treatment defined in (1) to (6) above, when yeast is used instead of lactic acid bacteria, “yeast fermentation processed product”, “yeast fermentation culture solution”, “yeast fermentation extract” "," Yeast fermented product ". In this case, “yeast” is sometimes referred to as “yeast”, but it is synonymous.

  The roots of yellow flowers used in the first step of the present invention are the genus forget-me-nots, Hemerocallis fulva var. Kwanso, Zentica (H. dumortieri var. Esculenta), H. fulva), Manchurian (H.flava L.), Hosobakisu (H.flava var. minor) and the roots of these related plants can be used. The root part used in the present application refers to the entire part called a root as a general concept, and it is also possible to take out and use a part of a stem extending into the ground such as an underground stem.

The solvent for obtaining the leachate in the first step of the present invention is water, lower alcohols (methanol, ethanol, propanol, etc.) or glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin, etc.) or these And a mixed solution thereof can be used. Moreover, it is preferable to add sugar sources, nutrients, trace growth factors, and the like necessary for the growth of lactic acid bacteria as needed. The mixing ratio (weight ratio) between the roots of yellow rape and the leachate is generally 1: 1 to 1: 1000, preferably 1: 5 to 1: 100, more preferably 1:10 in terms of dry weight of the root of yellow rape. It is in the range of ˜1: 50.

The leachate in the first step of the present invention is obtained as follows.
First, the root part of yellow rape to be leached is immersed or dispersed in the solvent. In this case, the roots of yellow rape may be used as they are, or may be used after being dried or semi-dried in advance. In addition, as the shape, the collected one can be used as it is, but if it is shredded or pulverized and refined, the fermentation efficiency can be increased in the subsequent fermentation process. In this invention, the said leaching liquid is provided to the fermentation process which is a next process, without removing the leached yellow flower rape root part. By inoculating and fermenting lactic acid bacteria, the tissue of the yellow rapeseed root can be decomposed by lactic acid bacteria. This makes it possible to extract components that could not be extracted by the extraction by the conventional method, and an unprecedented effect can be obtained. However, in the case where the leaching is sufficiently performed, the components can be sufficiently extracted from the root portion, so that the yellow rape root portion may be removed and used. In this case, the extracted component is fermented by lactic acid bacteria and converted into a new active ingredient. In that sense, even in a state corresponding to a normal plant extract, it can be used as a leachate.

The leachate obtained by leaching the roots of yellow flowers can be used for the fermentation process as it is. It is sufficient that the root part of yellow rape is leached in a solvent, and it is not necessary to provide a special leaching time. Before the leachate is subjected to a fermentation process, it is preferable to sterilize and remove miscellaneous bacteria that hinder fermentation. In this case, as a method for removing germs, the roots of yellow rape vegetables to be subjected to fermentation may be preliminarily washed and sterilized with ethanol for sterilization and then leached by a method of suspending in a sterile medium such as sterile water. A method may be used in which the root is leached in a solvent to obtain a leachate and then heat sterilized.
As the heat sterilization method, an autoclave sterilization method in which the fermented liquid added with yellow flower vegetables is heated at 105 to 121 ° C. for 10 to 20 minutes, or the fermented liquid added with yellow flower vegetables is maintained at 80 to 90 ° C. for 60 to 120 minutes. The intermittent sterilization method is generally used once a day for 2-3 days. However, this sterilization process is not an essential process. If it does not become an obstacle in the next fermentation process, this sterilization process may be omitted.

The bacterium used in the fermentation process which is the second process of the present invention is a lactic acid bacterium. The lactic acid bacteria used in the present invention are not particularly limited as long as they belong to lactic acid bacteria. It can be easily separated from commercially available yogurt or from nature. Moreover, since it is also distributed in the microorganism distribution agency, it is possible to purchase and use the strain. Separation can be performed by a method described in JP 2012-105639 A or the like.

  Specifically, lactic acid bacteria belonging to the genus Lactobacillus such as Lactobacillus plantarum, L. delbrueckii, L. brevis, L. casei, etc .; Lactic acid bacteria of the genus Leuconostoc such as Leuconostoc mesenteroides and L. citreum; Streptococcus such as Streptococcus faecalis and Streptococcus such as Streptococcus pyogenes Lactic acid bacteria; Enterococcus caseliflavus, Enterococcus lactic acid bacteria such as E. sulfreus; Lactococcus plantarum, Lactococcus plantarum, Lactococcus lactic acid bacteria such as L. rafinolactis; Pediococcus damnosus, Pediococcus genus such as P. pentosaceus It is done. Among these lactic acid bacteria, the use of Lactobacillus plantarum and Lactobacillus delbrueckii is most preferable from the viewpoint of the effectiveness of the obtained fermented product and the fact that it is not extremely anaerobic and easy to handle. . Since Lactobacillus delbrueckii is a typical lactic acid bacterium used for the production of yogurt, it can be easily separated from commercially available yogurt and from the natural world. Lactobacillus plantarum is said to be a plant lactic acid bacterium and is abundant in pickles and can be easily isolated.

As a culture medium for lactic acid bacteria, an MRS medium developed as a medium showing good growth of the entire lactic acid bacteria can be used. (MRS medium composition: peptone 10 g, beef extract 10 g, yeast extract 5 g, glucose 20 g, Tween 80 1 g, K ₂ HPO ₄ 2 g, sodium acetate 5 g, diammonium citrate 2 g, MgSO ₄ .7H ₂ O 0.2 g, MnSO ₄・ NH ₂ O 0.05g, purified water 1L)

As a medium composition of a lactic acid bacteria synthetic medium, it is sufficient to include a minimum carbon source, a nitrogen source and a phosphorus source. Examples of the carbon source include ribose, arabinose, xylose, glucose, fructose, mannose, galactose, and rhamnose. Disaccharides such as monosaccharides, sucrose, maltose, lactose, trehalose and cellobiose, and trisaccharides such as raffinose and maltotriose can be used. One or more of these may be contained. Nitrogen sources include urea, potassium nitrate as nitrate, sodium nitrate, calcium nitrate, ammonium salts as ammonium sulfate and ammonium chloride, and amino acids as tryptophan, lysine, methionine, phenylalanine, threonine, valine, isoleucine, leucine, histidine, alanine, Nitrogen-containing compounds such as arginine, asparagine, serine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, proline, and tyrosine can be used, and one or more of these may be contained. As the phosphorus source, phosphate can be used, and potassium monohydrogen phosphate, potassium dihydrogen phosphate, and ammonium phosphate can be used, and one or more of these may be contained.

The basic medium of the synthetic medium is sufficient if it contains the carbon source, nitrogen source and phosphorus source. However, since lactic acid bacteria have strict auxotrophy, vitamin sources and mineral sources may be added. is necessary. Examples of vitamin sources include biotin, thiamine (vitamin B1), riboflavin, pyridoxine (vitamin B6), pantothenic acid, ascorbic acid, iodic acid, cyanocobalamin, inositol, nicotinic acid, choline, carnitine, paraaminobenzoic acid, nicotinamide adenidine Nucleotides and the like can be used. Examples of mineral sources include potassium, calcium, magnesium, sulfur, iron, manganese, zinc, boron, copper, molybdenum, sodium, iodine, cobalt, and the like. Specific components that can supply these include, for example, calcium nitrate. , Potassium chloride, calcium chloride, magnesium sulfate, sodium sulfate, ferrous sulfate, ferric sulfate, manganese sulfate, zinc sulfate, boric acid, copper sulfate, sodium molybdate, molybdenum trioxide, potassium iodide, cobalt chloride, etc. These compounds can be used.

In the present invention, the above components are appropriately combined to form a basic medium. Of course, it is also possible to culture in a synthetic medium containing no natural components and in a medium in which a small amount of natural products are combined when the growth of microorganisms is poor.

Of course, the medium composition other than the above can be applied to the present invention as long as it is a substance capable of assimilating and growing lactic acid bacteria. Furthermore, a lower alcohol (methanol, ethanol, propanol, etc.) or glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin, etc.) or the like may be added to the medium to form a basic medium.

In the present invention, the lactic acid bacteria are stored in a dry state or a frozen state, and are cultured in advance in the basic medium or the like at the time of use. The culture at this stage may be performed as usual as long as each bacterium can grow.

The amount of inoculation in the inoculation step of lactic acid bacteria in the second step of the present invention is an appropriate amount of 10 ⁸ to 10 ¹⁰ cells / mL with respect to the leachate. Even if the inoculation amount exceeds the above range, the fermentation progress time hardly changes. On the other hand, if the inoculation amount is less than the above range, it takes a long time to complete the fermentation.

The fermentation temperature is generally in the range of 5 to 50 ° C, preferably in the range of 25 to 40 ° C, which is the optimum temperature for growth of lactic acid bacteria. The number of days of fermentation is generally in the range of 3 to 50 days, preferably 7 to 35 days, at the optimum temperature. When fermentation days become shorter than said general range, fermentation may not fully be performed but the effectiveness of fermented material may fall. On the other hand, even if it is longer than 35 days, the effectiveness beyond that is not recognized, which is not preferable. It is sufficient that the fermentation process is carried out by standing, but for shortening the fermentation time, it is also possible to carry out shaking culture and aeration culture. The leachate which passed through the fermentation process on the said conditions is called a fermentation processed material. At this stage, the solvent and the plant are mixed. When the above fermentation process is completed, in order to stop the fermentation, the fermented product is subjected to a heat sterilization process at 80 to 120 ° C. for about 15 to 120 minutes.

In the third step, the fermented product after the sterilization treatment is used as it is, or generally and preferably by separating the liquid phase by solid-liquid separation means such as filtration or centrifugation to obtain a fermentation broth. . The fermentation broth can be used as it is, but if necessary, it is diluted or concentrated to an appropriate concentration and used as a cosmetic raw material. In some cases, the liquid phase after solid-liquid separation is solidified according to conventional methods such as spray-drying and freeze-drying, and pulverized as necessary to form a powder and then blended into cosmetics. May be.

In the fourth step, water or lower alcohol (ethanol, propanol), or glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin, etc.) or these are added to the roots of yellow rapeseed separated in the third step. A mixed extract or the like is used as an extraction solvent to extract by heating to obtain a fermentation extract.
In addition, although the extraction temperature and extraction time at the time of obtaining a fermented extract can be suitably adjusted according to the objective, extraction temperature is also 80-120 degreeC also serving as a disinfection process, Preferably it is 90 to 105 degreeC. . The extraction time is 15 to 120 minutes, preferably 30 to 60 minutes.

The obtained fermented product can be used as a cell differentiation promoter, a barrier function improver, an SA-β-Gal activity inhibitor, and a DPPH radical scavenger. Cosmetics containing fermented products include basic cosmetics such as emulsions, creams, lotions, essences, packs, facial cleansers, and makeup makeup such as lipsticks, foundations, liquid foundations, and makeup pressed powders. And cosmetics such as cosmetics, facial cleansers, body shampoos, soaps and the like, as well as bath agents, but of course are not limited thereto.

The blending amount of the fermented product in the cosmetic of the present invention is in the range of 0.0001 to 10% by mass, preferably 0.01 to 1% by mass in terms of the evaporation residue of the obtained culture solution. .

In addition to the above-mentioned essential ingredients, the cosmetics of the present invention include compounding ingredients usually used in cosmetics such as oily ingredients, surfactants, moisturizers, thickeners, antiseptic / disinfectants, powder ingredients, ultraviolet rays. Absorbers, pigments, fragrances and the like can be appropriately blended as necessary.

Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty acid amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, etc. ON surfactant: quaternary ammonium salt, primary to tertiary fatty acid amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

Examples of the humectant include glycols such as glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sugars such as maltitol, sorbitol, xylitol, trehalose, glucose, sodium pyrrolidonecarboxylate, mucopoly Sugars (eg, hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, hydrolyzed silk protein, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, Examples include phytosterol, soybean phospholipid, cholesteryl isostearate, seaweed extract, various amino acids, and derivatives thereof.

Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, and fartheraran, green algae or red algae-derived components, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, gums such as xanthan gum, tragacanth gum, guar gum and the like , Cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose, synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives, glucosyl trehalose And sugar compounds mainly composed of hydrolyzed hydrogenated starch.

Examples of the antiseptic / bactericidal agent include paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride , Salicylic acid, ethanol, undecylenic acid, phenols, jarmal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark distillate, propolis extract and the like.

Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, 6- or 12-nylon powder, polyethylene powder. , Silk powder, cellulosic powder and the like.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

Furthermore, if necessary, other active ingredients (whitening agent, skin aging prevention / rough skin improvement agent, anti-inflammatory agent, etc.) may be blended within the range that does not impair the effects and features of the fermented product used in the present invention. Examples of such a whitening agent include tranexamic acid and its derivatives, t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone derivatives, ellagic acid and its derivatives, resorcinol derivatives, placental extraction , Cysteine, Sakuhakuhi extract, Yukinoshita extract, Hamamelis extract, Itadori extract, Licorice extract, Gentian extract, Jujube extract, Peonies extract, Sugi extract, Peach extract, Green algae, Red algae or Brown algae Seaweed extracts, linoleic acid and its derivatives or processed products (eg menthol linoleate) Ester, etc.), 2,5-dihydroxybenzoic acid derivatives, etc., are components for preventing skin aging and improving rough skin, collagen derived from animals or fish and derivatives thereof, elastin and derivatives thereof, intercellular lipids such as ceramide, placenta extraction Nicotinic acid and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A precursor, vitamin A and derivatives thereof, vitamin E and derivatives thereof, allantoin, α-hydroxy acids, diisopropyl Amine dichloroacetate, γ-amino-β-hydroxybutyric acid, coenzyme Q-10, adenosine, α-lipoic acid, picoline, carnitine and its derivatives, gentian extract, licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract, etc. Herbal medicine extract , Green algae, red algae or brown algae seaweed extract, Sakuhakuhi extract, beech extract, Kidachi aloe extract, mannenrou extract, ginkgo biloba extract, horsetail extract, safflower extract, rapeseed extract, elderberry extract For example, guaiazulene sulfonic acid can be used as an anti-inflammatory component, for example, Guagomogusa extract, astragalus extract, mango extract, eggshell membrane extract protein, deoxyribonucleic acid potassium salt, purple orchid root extract, purple plant extract, rice extract, etc. Azulene derivatives such as sodium and ethyl guaiazulenesulfonate, glycyrrhizic acid derivatives such as dipotassium glycyrrhizinate and stearyl glycyrrhetinate, allantoin, licorice extract, cucumber extract, peony extract, button pi extract, forsythia extract, ryutan extract Things, toe calendula extract, parsley extract, Hypericum extract, and the like. Antioxidants include, for example, superoxide dismutase, bioactive oxygen-degrading enzymes such as catalase, vitamins such as vitamin E and vitamin D and derivatives thereof, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, There are ubidecaquinone (ubiquinone), rutin, rutin glucoside, γ-oryzanol and the like.

Examples of fermented product effect tests in the present invention will be described below. Furthermore, although the application formulation example etc. to the skin composition using fermented material are described, it is not limited to the Example described here. In the following, all parts are parts by mass, and all% are% by mass.

Lactobacillus plantarum (NBRC 101975), which was sold by the National Institute for Product Evaluation Technology, was used as the lactic acid bacterium to inoculate the root exudate of yellow rape. Lactic acid bacteria were cultured in MRS medium, 30 ml of the medium was inoculated with 1 platinum loop of the strain that had been transferred, and statically cultured at 30 ° C. for 3 days to obtain a lactic acid bacteria culture solution. (MRS medium composition: peptone 10 g, beef extract 10 g, yeast extract 5 g, glucose 20 g, Tween 80 1 g, K ₂ HPO ₄ 2 g, sodium acetate 5 g, diammonium citrate 2 g, MgSO ₄ .7H ₂ O 0.2 g, MnSO ₄・ NH ₂ O 0.05g, purified water 1L)

Preparation of Fermentation Processed Product 1 g of glucose and 100 ml of purified water were added to 10 g of dried yellow rape roots and leached at room temperature. Thereafter, sterilization was performed at 105 ° C. for 15 minutes to obtain a leachate. After cooling, 5 ml of the lactic acid bacteria culture solution was added to the leachate and stirred well. The leachate in which the lactic acid bacteria were planted was subjected to stationary culture at 30 ° C. for 28 days to obtain a yellow flower rape root fermented product.

Preparation of Fermented Lactobacillus at the Root of Yellow Flower Vegetables The obtained fermented product is sterilized at 105 ° C. for 15 minutes in order to stop the fermentation process. After sterilization, the culture solution is squeezed out with a filter cloth. The filtrate was centrifuged at 6,000 rpm × 10 min., And the supernatant was taken as a fermentation broth for effect test. Further, purified water was added at a ratio of 10 ml to 1 g of yellow root vegetable residue remaining on the filter cloth, and sterilized and extracted at 105 ° C. for 15 minutes. After sterilization extraction, the extract was squeezed out with a filter cloth to obtain a yellow flower rape fermentation extract of the effect test. Further, as a negative control, a culture solution (“plant-free / fermented culture solution”) was prepared after culturing only with lactic acid bacteria without adding yellow flower vegetables in [0042].

In order to show that the fermented product obtained using the lactic acid bacterium in the present invention is superior to the fermented product obtained using the yeast, the same test was performed using the yeast.
Saccharomyces cereviciae (NBRC 0308), which was sold by the National Institute for Product Evaluation Technology, was used as the yeast for inoculating the root exudate of yellow rape. Yeast culture was performed by adding 2.0% glucose to YNB (Yeast Nitrogen Base) with synthetic medium (hereinafter referred to as YNB-SG medium) in 50 ml medium. 1 platinum ear was ingested, and static culture was performed at 25 ° C. for 5 days to obtain a yeast culture solution.
<YNB-SG medium composition>
Ammonium sulfate 5,000, potassium dihydrogen phosphate 1,000, magnesium sulfate 500, sodium chloride 100, calcium chloride 100, biotin 0.002, calcium pantothenate 0.4, folic acid 0.002, inositol 2.0, niacin 0 .4, 0.2 paraaminobenzoic acid, pyridoxine hydrochloride 0.4, riboflavin 0.2, thiamine hydrochloride 0.4, boric acid 0.5, copper sulfate 0.04, potassium iodide 0.1, ferric chloride 0.2, manganese sulfate 0.4, sodium molybdate 0.2, zinc sulfate 0.4, glucose 20,000 (unit is mg / L).

Preparation of Yeast Fermentation Treated Product 1 g of glucose and 100 ml of purified water were added to 10 g of roots of dried yellow rape and leached at room temperature. Thereafter, sterilization was performed at 105 ° C. for 15 minutes to obtain a leachate. After cooling, 5 ml of the yeast culture solution was added to the leachate and stirred well. The leachate in which the yeast was planted was subjected to stationary culture at 25 ° C. for 28 days to obtain a yeast fermented product.

Preparation of Fermented Yeast Fermented Product of Yellow Flower Vegetables The obtained fermented product is sterilized at 105 ° C. for 15 minutes in order to stop the fermentation process. After sterilization, the culture solution is squeezed out with a filter cloth. The filtrate was centrifuged at 6,000 rpm × 10 min., And the supernatant was taken to obtain a yeast fermentation culture solution for the root part of yellow rape of the effect test. Further, purified water was added at a ratio of 10 ml to 1 g of yellow root vegetable residue remaining on the filter cloth, and sterilized and extracted at 105 ° C. for 15 minutes. After sterilization extraction, the extract was squeezed out with a filter cloth, and used as a yeast fermentation extract of the root of yellow rape of effect test. Further, as a negative control, after culturing only with yeasts to which yellow flowers were not added in [0049] [0050], a culture solution subjected to centrifugation treatment was prepared.

<Cell differentiation promoting effect and barrier function improving effect>
In skin with an abnormal barrier function, there are many undifferentiated keratinocytes, and a normal stratum corneum is not formed. For this reason, skin inflammation is likely to occur, and this inflammation causes a vicious cycle in which the barrier function becomes abnormal.
Therefore, if cell differentiation can be promoted, an effect of improving the barrier function can be expected.
Then, the cell differentiation promotion effect of the lactic-acid-bacteria fermented material of the root part of yellow flower vegetables was confirmed.

Cell culture cells: Hacat cell culture medium: Humedia KG2
D-MEM (Ca concentration: 1.8 mM)
Fixing solution: Mildform 10NM (Wako Pure Chemical Industries)
Staining: 0.05% naphthol blue black solution (9% acetic acid, 0.1M sodium acetate)
Hacat cells, which are human epidermal cells, were cultured in Humedia KG2 (Kurabo) medium.
Cells were planted in a 12-well plate to about 50% confluence and cultured. The next day, each sample was added. After the addition, the cells were fixed after culturing for 72 hours, stained with a naphthol blue black solution, the morphology of the cells was observed with a microscope, and the degree of differentiation was determined.

Evaluation of cell differentiation When keratinocytes, which are epidermal cells, differentiate, the cells adhere to each other and take an amorphous form. On the other hand, it is generally known that undifferentiated cells are independent of each other and do not adhere to each other. Differentiation of keratinocytes is promoted by the Ca concentration in the medium. As shown in the photograph in [Fig.-1], when Hacat cells are cultured in a D-MEM medium with a Ca concentration of 1.8 mM, the cells are completely differentiated (corresponding to the photograph with the evaluation score of 5), and the Humedia KG2 medium with a low Ca concentration Then, the cells proliferate in an undifferentiated state (corresponding to the photograph with the evaluation score 1). The degree of adhesion between undifferentiated and differentiated cells was visually evaluated with a microscope in five stages to evaluate the degree of cell differentiation. The criteria for cell differentiation were determined according to the criteria shown in [Fig. 1].

[Table-1] shows the results of the cell differentiation degree when each sample was added based on the evaluation criteria of the cell differentiation degree evaluation in [Fig.-1]. The experiment was performed by adding 2.5% of the fermented product added to the cells to the medium prepared in [0042]. As comparative example-3, extract of yellow rapeseed before cultivation (hereinafter referred to as “yellow rapeseed / root / precultured extract”), and as comparative example-4, culturing was carried out only with lactic acid bacteria without adding yellow rapeseed, followed by centrifugation. Treated culture fluid (hereinafter referred to as “plant-free / fermented culture fluid”), Example-1 fermentation culture fluid for root of yellow rape (hereinafter referred to as “yellow rapeseed / root / fermented culture fluid”), Example- 2 shows the result of the fermented extract of the root of yellow rape (hereinafter referred to as “yellow rapeseed / root / fermented extract”). In addition, when showing the sample content, it shows similarly. The yellow flower vegetable / root / pre-culture extract of Comparative Example-3 showed a cell differentiation degree rating of 2 and showed almost no cell differentiation promoting effect. However, in Example-1 yellow flower vegetable / root / fermentation broth, the cell differentiation score was 5 points, and in Example-2, yellow flower vegetable / root / fermentation extract also showed 5 cell differentiation score. It showed a high cell differentiation promoting effect. Further, Comparative Example-4, which was tested as a negative control, had a cell differentiation degree score of 1, indicating that the lactic acid bacteria culture solution alone has no cell differentiation promoting effect. In addition, the cell differentiation degree score of the undifferentiated cells cultured in the Humedia KG2 medium of Comparative Example-2 was determined as 1, and the cell differentiation degree score of the differentiated cells cultured in the D-MEM medium was determined as 5. From the above results, it was found that the components extracted from yellow flower vegetables and roots were changed by fermentation treatment with lactic acid bacteria, and the effect of promoting cell differentiation was enhanced.

<SA-β-Gal activity inhibitory effect / cell aging improvement effect>
Decrease in skin functions such as elasticity, elasticity and sagging that progress with aging is caused by aging at the cellular level.
On the other hand, in human skin fibroblasts, it is known that SA-β-Gal activity (senescence-associated beta-galactosidase) in cells increases with the passage of cells and the age of collected humans. -β-Gal activity has been used as a cellular senescence marker.
Therefore, if the SA-β-Gal activity can be suppressed, it is considered that a cell aging inhibitory effect can be expected.
Therefore, the SA-β-Gal activity of the fermented metabolite of lactic acid bacteria at the root of yellow rape was confirmed.

Measurement of cell aging by measuring SA-β-Gal activity Cultured cells: Normal human fibroblasts NB1-RGB (RIKEN)
Medium: D-MEM FBS 10%
Normal human fibroblasts NB1-RGB were cultured, and 200 μM hydrogen peroxide was added to the cell culture medium for 2 hours a day. This was repeated for 2 days in succession to cause cell senescence. After the sample prepared in [0042] was added to the cells at a concentration of 2.5% for 3 days, SA-β-Gal staining was performed, and the degree of cell aging was measured based on the degree of cell staining. Note that nicotinamide was used as a positive control.
Fix cells with SA-β-Gal staining fixative for 5 minutes. After washing the cells with PBS (−), an SA-β-Gal solution was added and left at 37 ° C. for 16 hours. Cells stained with the SA-β-Gal staining solution were collected, dissolved in 2N-NaOH solution, the absorbance at 655 nm was measured (A value), and SA-β-Gal activity was measured. A part of the lysate was measured for absorbance at 540 nm (B value) by the BCA method, and the amount of protein was measured. The SA-β-Gal activity per cell protein amount was determined by the following calculation formula.

SA-β-Gal activity = absorbance value at 655 nm (A value) / absorbance value at 540 nm measured by BCA method (B value)

Fixing solution: 95 mL of PBS (−) is added to 5 mL of formaldehyde solution (reagent formalin 37% concentration).
X-Gal solution: 20 mg of X-Gal (Wako Pure Chemical Industries) is dissolved in 1 mL of DMSO.
SA-β-Gal staining solution: Dissolve in the following staining buffer so that X-Gal has a concentration of 1 mg / mL.
Staining Buffer: 40 mM citric acid: 40 mM disodium hydrogen phosphate (1: 3)
The following 4 reagents are dissolved in an aqueous solution adjusted to pH 6.0.
5 mM potassium ferrocyanide
5 mM potassium ferricyanide 150 mM NaCl
2 mM MgCl ₂
BCA method: The following C liquid and D liquid are mixed at a ratio of 50: 1 immediately before measurement.
To 40 μL of cell lysate dissolved in 2N-NaOH, 200 μL of a mixture of solution C and solution D is added and reacted at 37 ° C. for 30 minutes, and then the absorbance at 540 nm is measured to determine the amount of protein.
Liquid C Sodium bicinchoninate 10g
Na ₂ CO ₃ 20g
Sodium tartrate 1.6g
NaOH 4g
9.5 g of NaHCO ₃
After dissolving with purified water, the pH is adjusted to 11.25 with 10N NaOH, and then adjusted to 1L.
D solution CuSO ₄ · 5H ₂ O 4g
Make up to 100 mL with purified water.

[Table-2] shows the results of cell aging degree by measuring SA-β-Gal activity. SA-β-Gal activity score was 13.8 in cells that were treated twice for 2 hours at a concentration of 200 μM hydrogen peroxide in Comparative Example-5. On the other hand, SA-β-Gal activity score was 5.36 in the cell of Comparative Example-6 which was not treated with hydrogen peroxide. In addition, in the cells cultured in hydrogen peroxide-treated 200 μM concentration in Comparative Example 7 twice for 2 hours and then added with 5 mM nicotinamide, the SA-β-Gal activity score is 5.63. It was found that the degree of cellular aging was suppressed by the addition of 5 mM nicotinamide. Furthermore, in Comparative Example-8 to which yellow flower vegetables, roots and pre-culture extract were added, the SA-β-Gal activity score was 5.61 points. In addition, Comparative Example 9 tested as a negative control had an SA-β-Gal activity score of 13.3, indicating that there was no cell aging inhibitory effect only with the culture solution of lactic acid bacteria. However, the SA-β-Gal activity score was 4.78 points in Example-3 added with yellow flowers, roots, and fermentation broth, and as Example-4, SA-β-Gal activity score was added in SA- The β-Gal activity score was 2.69, indicating a very high cell aging inhibitory effect. From these results, it was found that the components extracted from yellow flowers and roots were changed by fermentation treatment with lactic acid bacteria, and the effect of inhibiting cell aging was enhanced.

<Radical scavenging effect>
Reactive oxygen generated in the cell damages the cell by causing irreversible chemical changes in the components that make up the cell, and the damage accumulates with age, leading to a decrease in cell function, leading to cell aging. It is known to connect.
On the other hand, DPPH (diphenylpicrylhydrazyl) is an artificially generated stable radical and is generally used as a screening test for antioxidants.
Therefore, it is considered that a substance capable of scavenging DPPH radicals can be expected to prevent oxidative deterioration of cell constituents due to active oxygen, prevent a decrease in cell function, and further suppress aging.
Therefore, the DPPH radical scavenging activity of lactic acid bacteria fermentation metabolites in the roots of yellow flowers was confirmed.

Samples prepared in DPPH radical scavenging activity test [0042] and 0 mM, 0.3 mM, 0.5 mM, 0.7 mM, 1.0 mM, 3.0 mM, 5.0 mM, 10 mM Trolox (6-hydroxy-2,5 , 7,8-tetramethylchroman-2-carboxylic acid) solution was added 190 μL of DPPH solution prepared at the following rate, and after 15 minutes, the absorbance at 540 nm was measured to evaluate DPPH radical scavenging ability. A calibration curve was prepared from the absorbance of Trolox solution of known concentration, and the strength of DPPH radical scavenging ability of the sample was displayed as trolox equivalent.
Trolox solution: 25 mg of trolox was dissolved in 10 ml of DMSO (Dimethyl sulfoxide) to prepare a trolox 10 mM solution.
DPPH solution 0.4 mM-DPPH (1,1-Diphenyl-2-picrylhydrazyl) 40
400 mM MES buffer (2-Morpholinoethanesulfonic acid) (PH6.1) 10
Distilled water 30

The results of DPPH radical scavenging ability are shown in [Table-3].
In Comparative Example-10 to which yellow flower vegetable, root, and pre-fermentation extract were added, the DPPH radical scavenging ability was 0.37 mM trolox equivalent. However, 0.94 mM trolox equivalent in Example 5 added with yellow flowers, roots and fermentation broth, and 0.72 mM trolox equivalent in Example 5 added with yellow flowers, roots and fermentation extract. It showed a high oxidation inhibition effect. In Comparative Example 11 tested as a negative control, the DPPH radical scavenging ability was 0.11 mM trolox equivalent, and it was shown that there was no DPPH radical scavenging activity only in the culture solution of lactic acid bacteria. Further, as a comparative control, Comparative Example-12 showed the DPPH radical scavenging ability of the yeast fermentation broth obtained by fermenting the roots of yellow rape with yeast. Since the DPPH radical scavenging ability of Comparative Example-12 was 0.39 mM trolox equivalent, it was found that the DPPH radical scavenging effect by the fermentation treatment of yeast was low.
The DPPH radical scavenging ability of Comparative Example-13, which was tested as a negative control, was 0.13 mM trolox equivalent, and it was also shown that there was no DPPH radical scavenging activity in the yeast culture alone.
From these results, it was clarified that the DPPH radical scavenging activity increased due to the change in the components extracted from yellow flower vegetables and roots by fermentation treatment with lactic acid bacteria.

As human test subjects, 10 females aged 20 to 60 years were allowed to use the skin preparations for the test and comparison products twice a day (morning and evening) for 1 month in succession. The state was compared before and after the test, and the improvement effect was investigated. In this test, the skin external preparation shown in Formulation Example 7 was used as a test product (Example-7), and the comparative product was a lactic acid bacteria fermented product (yellow rape, root, fermentation) from the skin external preparation shown in Formulation Example 7. Except for the culture solution and the fermentation extract), an external preparation for skin was prepared by replacing yellow flower vegetables, roots, and the pre-fermentation extract (Comparative Example-12), and the effects of its use were examined. Evaluation was performed according to the criteria shown in Table 4 for evaluation items related to skin elasticity and Table 5 for evaluation items related to skin moisture. While using the external preparation for skin containing the active ingredient of the present invention every day, the skin condition was tabulated before and after the application for 1 month to confirm the effect. The results are shown in Tables 6 and 7. The numbers in the table indicate the number of people. As is clear from Table-6, the skin external evaluation agent containing the fermented lactic acid bacteria showed a skin elasticity evaluation score of 78 points. On the other hand, in the comparative product in which the fermented product was removed from the test product and replaced with yellow flower vegetables, roots and the pre-fermentation extract, the evaluation score was 38 points. As is clear from this result, it was revealed that a high skin elasticity effect can be obtained by adding a fermented product of lactic acid bacteria. Furthermore, according to Table-7, in the skin external preparation of the test product containing the lactic acid bacteria fermented product, the skin moisturizing evaluation score was 80 points. On the other hand, the comparative product in which the fermented product was removed from the test product and replaced with yellow flower vegetables, roots, and the pre-fermentation extract had an evaluation score of 40 points. As is clear from this result, it was revealed that a high skin moisturizing effect can be obtained by adding fermented lactic acid bacteria.

Next, although the formulation example which mix | blended the fermented material of this invention is shown, this invention is not limited to this.

Examples of cosmetic preparations The fermentation treatment liquids shown in the following examples of cosmetic preparations represent the respective culture solutions and extracts prepared by the method shown in [0042]. The “fermentation culture solution (yellow flower vegetables / root)” means “the lactic acid bacteria fermentation culture solution of the root of yellow flower vegetables”. Hereinafter, it shows similarly.
(Formulation Example 1) Cosmetic cream (mass%)
a) Beeswax 2.0
b) Stearyl alcohol ... 5.0
c) Stearic acid ... 8.0
d) Squalane ... 10.0
e) Self-emulsifying glyceryl monostearate 3.0
f) Polyoxyethylene cetyl ether (20E.O.) ... 1.0
g) Fermentation broth (yellow rape and root) ... 5.0
h) Fermented extract (yellow rape and root) ... 5.0
i) 1,3-butylene glycol ... 5.0
j) Potassium hydroxide ... 0.3
k) Preservatives / Antioxidants: appropriate amount
l) Heat and dissolve purified water: remaining manufacturing methods a) to f) and keep at 80 ° C. Heat up to i) to l), keep at 80 ° C., add to a) to f), emulsify, and cool to 40 ° C. with stirring. Thereafter, g) and h) are added and stirred to dissolve uniformly.

Cosmetic Formulation Example (Formulation Example 2) Cosmetic Cream (mass%)
a) Beeswax 2.0
b) Stearyl alcohol ... 5.0
c) Stearic acid ... 8.0
d) Squalane ... 10.0
e) Self-emulsifying glyceryl monostearate 3.0
f) Polyoxyethylene cetyl ether (20E.O.) ... 1.0
g) Fermentation broth (yellow rape, root) ... 10.0
h) Fermented extract (yellow rape and root) ... 5.0
i) 1,3-butylene glycol ... 5.0
j) Potassium hydroxide ... 0.3
k) Preservatives / Antioxidants: appropriate amount
l) Heat and dissolve purified water: remaining manufacturing methods a) to f) and keep at 80 ° C. Heat up to i) to l), keep at 80 ° C., add to a) to f), emulsify, and cool to 40 ° C. with stirring. Thereafter, g) and h) are added and stirred to dissolve uniformly.

(Prescription Example 3) Emulsion (mass%)
a) Beeswax 0.5
b) Petrolatum 2.0
c) Squalane ... 8.0
d) Sorbitan sesquioleate ... 0.8
e) Polyoxyethylene oleyl ether (20EO) 1.2
f) Fermentation extract (yellow flower vegetables / root) ... 0.1
g) Fermentation broth (yellow rape, root) ... 1.0
h) 1,3-butylene glycol 7.0
i) Carboxyvinyl polymer 0.2
j) Potassium hydroxide ... 0.1
k) Purified water: balance
l) Preservatives / Antioxidants: appropriate amount
m) Ethanol: 7.0
The production methods a) to e) are dissolved by heating and kept at 80 ° C. Heat up to h) to k), keep at 80 ° C., add to a) to e), emulsify, and cool to 50 ° C. with stirring. Add f), g), m) at 50 ° C., stir to 40 ° C. and cool.

(Formulation Example 4) Lotion (mass%)
a) Fermented extract (yellow flower vegetables / root) ・ ・ ・ 0.01
b) Fermentation broth (yellow rape, root) ... 0.001
c) Glycerin ... 5.0
d) Polyoxyethylene sorbitan monolaurate (20E.O.) ... 1.0
e) Ethanol ... 6.0
f) Fragrance: appropriate amount
g) Preservatives / Antioxidants ...
h) Purified water: The remaining preparations a) to h) are mixed and dissolved uniformly.

(Formulation Example 5) Lotion (mass%)
a) Fermentation broth (yellow flower vegetables / root) ... 0.001
b) Fermentation extract (yellow flower vegetables / root) ... 10.0
c) Glycerin ... 5.0
d) Polyoxyethylene sorbitan monolaurate (20E.O.) ... 1.0
e) Ethanol ... 6.0
f) Fragrance: appropriate amount
g) Preservatives / Antioxidants ...
h) Purified water: The remaining preparations a) to h) are mixed and dissolved uniformly.

(Formulation Example 6) Face wash (mass%)
a) Stearic acid ... 12.0
b) Myristic acid 14.0
c) Lauric acid ... 5.0
d) Jojoba oil ... 3.0
e) Glycerin 10.0
f) Sorbitol ... 15.0
g) 1,3-butylene glycol ... 10.0
h) POE (20) Glycerol monostearic acid 2.0
i) Potassium hydroxide ... 5.0
j) Water ... balance k) chelating agent ... appropriate amount l) perfume ... appropriate amount m) fermented extract (yellow rape, root) ... 1.0
n) Fermentation broth (yellow rape, root) ... 1.0
The manufacturing methods a) to h) are dissolved by heating and maintained at 70 ° C. After i) is dissolved in j), it is added to a) to h) and saponified. Then, k) and l) are added and cooled with stirring. Add m), n) at 50 ° C., stir to 40 ° C. and cool.

(Prescription Example 7) Essence (mass%)
a) Fermented extract (yellow rape, root) ... 2.0
b) Fermentation broth (yellow flowers, roots) ... 2.0
c) Carboxyvinyl polymer ... 0.05
d) L-arginine ... appropriate amount
e) Glycerin ... 5.0
f) Polyoxyethylene sorbitan monolaurate (20E.O.) ... 1.0
g) Ethanol ... 6.0
h) Fragrance: appropriate amount
i) Preservatives / Antioxidants ...
j) Purified water: The rest of the production method c) is dispersed in a part of j), and d) is added to adjust the pH to 6.5. Then, a) to j) are mixed and dissolved uniformly.

In the present invention, the leaching solvent and the extraction solvent used for the leaching solution, the extraction solution and the like are all purified water unless otherwise indicated, but the purified water is used regardless of the type of the extraction solvent. The same effect as that obtained was obtained. In addition, when yellow rape is not particularly shown, it shows the case of using all edible licorice, but the same effect was obtained when using other zentika (Nikkoukigege), Honkanzo, Manshuukigege, Hosobakigege .

As described above in detail, according to the present invention, the aging of fibroblasts is suppressed by one active ingredient, the elasticity of the skin is restored, wrinkles and sagging are reduced, and cell differentiation is promoted. This makes it possible to improve the texture and restore the skin barrier function.

Claims (6)

A cell differentiation promoting agent comprising, as an active ingredient, a fermented product obtained by fermenting a root of yellow rape with lactic acid bacteria. A barrier function improving agent comprising a fermented product obtained by fermenting a root of yellow rape with lactic acid bacteria as an active ingredient. A SA-β-Gal activity inhibitor characterized by containing as an active ingredient a fermented product obtained by fermenting the roots of yellow flowers with lactic acid bacteria. A radical scavenging promoter characterized in that a fermented product obtained by fermenting the roots of yellow flower vegetables with lactic acid bacteria is blended as an active ingredient. The agent according to any one of claims 1 to 4, wherein the lactic acid bacterium is Lactobacillus plantarum. A cosmetic comprising a fermented product obtained by fermenting a root portion of yellow rape with lactic acid bacteria as an active ingredient.