JP2015044773A - Preventing and improving agent for skin aging caused by blue light irradiation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel and useful agent for suppressing the cell growth inhibition caused by irradiation of blue light, and to provide a composition useful for alleviation, prevention and improvement of skin aging including wrinkles and sagging.SOLUTION: The invention provides an agent for suppressing the cell growth inhibition, containing bilberry extract as an active ingredient, and a skin aging preventing and improving agent based on a cell growth inhibition suppressing action, and further various compositions containing the agent for suppressing the cell growth inhibition (pharmaceutical compositions, food and drink compositions, cosmetic compositions). For the bilberry, a drug of Vaccinium myrtillus L., Vaccinium, Ericaceae is used.

Description

  The present invention prevents or improves the prevention of the growth of skin constituent cells and the promotion of skin aging by repeatedly irradiating light in the blue light region derived from sunlight, computer displays, smartphones, etc. The present invention relates to a skin aging preventive agent, an improving agent, and an external preparation composition containing the same.

  Due to recent advances in IT technology, it has become an era when a computer and a mobile phone represented by a smartphone have multiple kitchens.

  At the same time, along with the technical confirmation of energy saving and higher resolution, LEDs are very often used for the backlight of LCD screens. It has been pointed out that the LED includes many regions called blue light having a wavelength of 380 nm to 495 nm as compared with a conventional liquid crystal backlight.

  Blue light has also been pointed out to increase concentration because it awakens the brain, but on the other hand, it is also caused by fatigue and poor physical condition due to excessive arousal action. For this reason, recently, products for reducing the burden on the eyes have been put to practical use, such as the release of computer glasses that cut the blue light area coming out of the PC display.

  The present invention provides a skin external preparation and a health food material that clarifies the growth inhibitory action of blue light on skin cells and prevents or improves the inhibitory activity.

  In addition, it is already known that the bilberry extract used in the present invention is used as a glutamate decarboxylase activator or a glutathione production promoter. (Patent Document 1)

JP 2006-241062 A

  Blue light is also caused by fatigue and poor physical condition due to excessive arousal action. When the effect on skin cells was examined, cell growth inhibitory activity was observed by irradiation with blue light. The aim was to protect the skin from the negative effects of this blue light.

In view of such a situation, the present inventor has developed a variety of plant extracts using active ingredients from naturally-occurring components that are natural and have excellent transdermal absorbability, stability, and safety. We investigated and repeated earnest examination.
As a result, the present inventors have confirmed that a specific plant extract (bilberry extract) suppresses the growth inhibition of skin cells caused by blue light irradiation.

  In addition, when repeated irradiation with blue light was repeated, it was confirmed that the inhibition of skin cell growth was also suppressed.

The present invention includes the following items.
[1] An inhibitor of cell growth inhibition by blue light comprising bilberry extract as an active ingredient.
[2] A skin aging preventive / ameliorating agent comprising the cell growth inhibition inhibitor by blue light according to [1].
[3] A pharmaceutical composition comprising the inhibitor of cell growth inhibition by blue light according to [1].
[4] A food / beverage composition comprising the inhibitor of cell growth inhibition by blue light according to [1].
[5] A cosmetic composition comprising the inhibitor of cell growth inhibition by blue light according to [1].

  The cell growth inhibition inhibitor by blue light which is the product of the present invention can be applied to various forms of preparations such as external preparation compositions, pharmaceutical compositions, quasi-drug compositions, foods and drinks, etc. Various symptom associated with mechanical aging and photoaging, such as wrinkles and sagging, can be expected to be prevented and improved.

It is a figure which shows the effect | action of the cell growth inhibition suppression by the blue light using the bilberry extract of this invention. The effects of other plant extracts (raspberry extract, Sakuhakuhi extract) were also shown. It is a figure which shows a cell growth inhibition inhibitory effect when the cell growth inhibition test by the blue light using the bilberry extract of this invention is repeated.

  The “bilberry” used in the present invention uses leaves of the plant of the family Acaceae and Vaccinium: Vaccinium myrtillus L.

  Examples of the extraction solvent for obtaining the bilberry extract used in the present invention include water, lower alcohols such as methanol, ethanol, propyl alcohol, isopropyl alcohol, butanol, and isobutanol, or hydrous lower alcohols, propylene glycol, 1,3- Butylene glycol, 1,2-butylene glycol, 1,4-butylene glycol, 1,5-pentanediol, 1,2-pentanediol, 1,3-pentanediol, 1,4-pentanediol, 1,3,5 -Polyhydric alcohol or hydrous polyhydric alcohol such as pentanetriol, glycerin, polyethylene glycol (molecular weight 100 to 100,000), acetone, ethyl acetate, diethyl ether, dimethyl ether, ethyl methyl ether, dioxane, acetonitrile, xylene, benzene, chloroform, Carbon tetrachloride, phenol, In various organic solvents such as ruene, acids (hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, formic acid, acetic acid, etc.) and alkalis (sodium hydroxide, potassium hydroxide, calcium hydroxide, ammonia, etc.) prepared as appropriate 1 type or 2 types or more of mixed liquids are mentioned, It is preferable to select 1 type, or 2 or more types especially chosen from water, ethanol, and 1, 3- butylene glycol. However, if it is not desirable to use a solvent depending on the application, use water alone or perform extraction using a highly volatile solvent that is easy to remove the solvent after extraction, and dissolve in water after removing the solvent. Such a method is also possible.

  Regarding the extraction method, the temperature of the solvent, the weight ratio of the solvent to the raw material, or the extraction time can be arbitrarily set for various raw materials and the solvent used. The temperature of the solvent at the time of extraction can be arbitrarily set in the range of −4 ° C. to 100 ° C., but is preferably around 10 to 40 ° C. from the viewpoint of the stability of the components contained in the raw material. Also, the weight ratio of the solvent to the raw material can be arbitrarily set, for example, in the range of the raw material: solvent 4: 1 to 1: 100, and the weight ratio of 1: 1 to 1:20 is particularly preferable.

  The bilberry extract used in the present invention is subjected to solvent extraction, decomposition by addition of acid (hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, organic acid, etc.) or alkali (sodium hydroxide, calcium hydroxide, ammonia, etc.), fermentation by microorganisms. Alternatively, processing such as metabolic conversion may be performed. The extract thus obtained can be appropriately subjected to purification operations. The purification operations include component adsorption by ion exchange resin, activated carbon, diatomaceous earth, etc., various separation modes (ion exchange, hydrophilicity). Fractionation using chromatography having adsorption, hydrophobic adsorption, size exclusion, ligand exchange, affinity, etc.), filtration using filter paper, membrane filter, ultrafiltration membrane, etc., followed by pressurization or Depressurization, heating or cooling, drying, pH adjustment, deodorization, decolorization, long-time storage and the like can be exemplified, and it is possible to perform a treatment by arbitrarily selecting and combining them.

  In producing the various compositions of the present invention, the shape of the bilberry extract to be used may be any shape such as liquid, solid, powder, granule, paste, etc., which is optimal for carrying out the present invention. Any shape can be selected arbitrarily.

  The bilberry extract of the present invention can be used as a cell growth inhibition inhibitor as it is, and further can be blended into a skin aging prevention / improving agent, a pharmaceutical composition, a food / beverage composition, and a cosmetic composition. The range is not particularly limited as long as the cell growth inhibition inhibitory action by light can be confirmed, and is generally set in the range of 0.01 mg / g to 200 mg / g (the denominator indicates the weight of the preparation). Preferably it is the range of 0.1 mg / g-20 mg / g, Most preferably, it is 200 microgram / g-10 mg / g.

  Specific dosage forms of the skin aging preventive / ameliorating agent of the present invention include creams, ointments, gels, lotions, solutions, packs, foundations, and the like. Various oils, surfactants, gels are used in these dosage forms. Agents, preservatives, antioxidants, solvents, alcohols, chelating agents, thickeners, dyes, fragrances, water and the like can be blended.

  Moreover, as an application method as a pharmaceutical composition, either oral administration or parenteral administration can be employed. In administration, the active ingredient can be mixed with a solid or liquid nontoxic pharmaceutical carrier suitable for administration methods such as oral administration, rectal administration and injection, and administered in the form of a conventional pharmaceutical preparation. Examples of such preparations include solid preparations such as tablets, granules, powders and capsules, liquid preparations such as solutions, suspensions and emulsions, freeze-dried preparations, and the like. It can be prepared by conventional means. Examples of the non-toxic pharmaceutical carrier include glucose, lactose, sucrose, starch, mannitol, dextrin, fatty acid glyceride, polyethylene glycol, hydroxyethyl starch, ethylene glycol, polyoxyethylene sorbitan fatty acid ester, amino acid, gelatin, Examples include albumin, water, and physiological saline. If necessary, conventional additives such as a stabilizer, a wetting agent, an emulsifier, a binder, and a tonicity agent may be added as appropriate.

  Moreover, as a food-drinks composition, it adds as a foodstuff for health or a health food, a supplement, and a food material as it is or it adds various nutritional components and makes it contain in food-drinks. For example, after adding the above-mentioned appropriate auxiliaries, it may be used for edible by using conventional means, and forming into an edible form, for example, granular, granular, tablet, capsule, paste, etc. In addition, various foods such as processed marine products such as kamaboko and chikuwa, livestock products such as sausage and ham, Western confectionery, Japanese confectionery, raw noodles, Chinese noodles, boiled noodles, buckwheat noodles, sauce, soy sauce, sauce, Seasonings such as sugar, honey, powdered candy, syrup, etc., spices such as curry powder, mustard powder, pepper powder, jam, marmalade, chocolate spread, pickles, red vegetables, sprinkles, canned and bottled vegetables and fruits Processed vegetables and fruits, dairy products such as cheese, butter, yogurt, miso soup, soup, fruit juice, vegetable juice, whey beverage, soft drink, alcoholic beverages, liquid food, etc. Addition to can be used, also, pet food, can also be used for animals and plants for feed and the like.

  The cosmetic composition can be used in, for example, various external preparations (including preparations used for animals) in general, specifically, ampoules, capsules, pills, tablets, powders, granules, solids 1) Pharmaceuticals, 2) Quasi-drugs, 3) Topical or systemic skin cosmetics (e.g., liquids, gels, bubbles, emulsions, sheets, mists, sprays, etc.) Basic cosmetics such as lotion, milky lotion, cream, ointment, lotion, oil, pack, facial cleanser and skin cleanser, massage agent, cleansing agent, hair remover, hair remover, shaving treatment, after shave lotion, Pre-show lotion, shaving cream, foundation, lipstick, blusher, eye shadow, eyeliner, mascara and other makeup cosmetics, perfumes, beauty nails, beauty nails enamel, beauty Enamel removers, poultices, plasters, tapes, sheets, patches, aerosols, etc.), 4) medicinal and / or cosmetic preparations applied to the scalp and hair (eg shampoos, rinses, Hair treatment agent, pre-hair treatment agent, permanent solution, hair dye, hair styling agent, hair styling agent, hair growth / hair nourishing agent, poultice agent, plaster agent, tape agent, sheet agent, patch, aerosol agent, etc.), 5) bath Bathing agents used in hot water, 6) Other anti-smell and deodorants, deodorants, antiperspirants, hygiene products, sanitary cotton, wet tissue, toothpaste, mouth freshener, gargle, dishwashing It can also be used for agents.

  In addition, in addition to the bilberry extract of the present invention that is an essential active ingredient, the cell growth inhibition inhibitor, the skin aging preventive / improving agent of the present invention, various compositions, in a range not impairing the effects of the present invention, The components and additives exemplified below can be arbitrarily selected and used in combination, and various functions can be added. The content in the preparation is not particularly limited, but a concentration range of usually 0.0001 to 50% is preferable.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, beef tallow fatty acid, cucumber oil, safflower oil , Shea fat, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, owl, palm Oil, beef tallow, pork tallow, squalene, squalane, pristane or hydrogenated products of these oils (hardened oil, etc.).

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax and the like.

(3) Mineral oils Liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcrystalline wax, etc.

(4) Fatty acids Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid, eicosapentaenoic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid Synthetic fatty acids such as natural fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) Alcohols: natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, phenoxyethanol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol .

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol 1,3-butylene glycol, pentyl glycol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, mannitol, lactitol, maltitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate, Diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) gum, sugar or water-soluble polymer compound gum arabic, benzoin rubber, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose or ester thereof, Trehalose or derivatives thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan to which alkylene (C2-C4) oxide such as ethylene oxide is added, low molecular chitin Or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or salt thereof, hyaluronic acid or salt thereof, chondroitin sulfate or salt thereof, heparin, ethylcellulose, methylcellulose, carboxy Polyalkylene such as methyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide and polypropylene oxide Oxide or its crosslinked polymer, carboxyvinyl polymer, polyethyleneimine and the like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate ester salt, alkyl phosphate ester salt), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt), amphoteric Surfactant: Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant ( Ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants ( Natural surfactants, protein hydrolyzate derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbons System surfactant), and the like.

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: vitamin C acid or derivatives thereof, vitamin D group: Ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group: vitamin E or derivatives thereof, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), me Dione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, .gamma.-oryzanol, orotic acid, vitamin P such (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine and the like, and their amino acid derivatives such as sulfate, phosphate, nitrate, citrate or pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a conventional manner according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, fermentation, purification) , Compression, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc., arbitrarily selected and combined), arbitrarily selected from various materials You can use it.

  The solvent used for extraction may be selected in consideration of the intended purpose and type of the product to be provided or the processing to be performed later. Usually, water, methanol, ethanol, propyl alcohol, isopropyl alcohol, butanol 1 type selected from various organic solvents such as lower alcohols such as isobutanol or hydrous lower alcohols, polyhydric alcohols such as propylene glycol, 1,3-butylene glycol and glycerin or hydrous polyhydric alcohols, acetone and ethyl acetate Alternatively, it is desirable to use a mixture of two or more. However, when it is not preferable to contain an organic solvent depending on the application, it is sufficient to use only water or ethanol that can be easily removed after extraction, and it may be used alone or in any mixture with water. The thing extracted and extracted may be sufficient.

  In addition, when using additives derived from plant or animal-based ingredients for systemic or topical external preparations and cosmetics, it protects skin and hair, moisturizes, improves touch and texture, imparts flexibility, and stimulates Relaxation, stress relief by fragrance, cell activation (cell aging prevention), suppression of inflammation, improvement of skin and hair quality, prevention of rough skin and its improvement, hair growth, hair growth, hair loss prevention, imparting gloss, cleansing effect, In addition to cosmetic effects such as fatigue relief, blood flow promotion, and warm bath effects, effects such as flavoring, deodorization, thickening, antiseptic, and buffering can also be expected.

  In addition, in food and drink, nutritional supplementation, fatigue recovery, tonicity, cell activation (cell aging prevention) and other healthy and cosmetic effects, as well as improved taste, color tone, aroma, gloss, stabilization, thickening, It can be used for antiseptic purposes. In addition to this, we expect various cosmetic and pharmaceutical effects of each of the raw materials known so far, and by combining these, we aim to enhance the target effect or to have a multifunctional effect It is also possible to make a product that expects

Specific plants (herbal medicines) as raw materials include, for example, almonds (toen), ai (indigo leaves), aokazura (Keifu Fuji), Aoki (Aoki), Aogiri or Kenashia Ogiri (Ao Tung), Aotsu Samurai (wooden candy) , Aotenma, acacia, red ginseng, red spruce `` fruit '', red clover, red grapes, red ginseng (logwood), red wrinkles (red buds), red crested or red crested or reddish (root), red crested or reddish (ground yellow), Agi (Aki), Aki larch, Akinire (crusted skin), Aguay Guassou, Aguahe (Omiten coconut palm), Akebi (Kitsu), Asa (Asakojin), Maruba morning glory or morning glory (Chicken), Hydrangea (hydrangea), Ashitaba (Tomorrow), Azuki (Red Azuki), Asunaro, Acerola, Asenyaku (Asenyaku), Athira (Shokuyokanna), Anise, Abiyu, A Biurana, oilseed rape, Abemaki `` fruit '', avocado, ama, achacha (Amacha), Achacharu, Amadokoro (Jade bamboo), Amana (a light-hearted), amaranth (Hyuyu, Hagatetou, Himogeito, Senninkoku, Sugimorigateto, Hosoaogeito, Hasoagoito Aozulan, Amaranthus hypochondriacus), Amigasayuri or Baimo (shellfish mother), Aristolomeria (Yurizuisen), Arcana (Alcanna), Algarrobo (Cabe), Altea, Arnica, Alpinia or Katsumadai (Suzukuku), Aloe (蘆薈), Aloe vera, angelica, apricot or honanzu (Anzujin), Ansokkou (benzoic incense), yellow support, igakozolina (geo-biliary head), licorice or gecko licorice (indian oyster), rush (light heart grass), itadori (tiger cane root), yew (one Place), Strawberry, fig (no flower `` fruit, leaf ''), Ichihatsu (first), Ichibi (winter lion), Ichiyakusou (one medicinal herb), ginkgo (Ginkgo `` seed, leaves ''), Japanese yellow peas (distant), carob, Japanese beetle ( Taillexi), Inubiyu (Honagainubiyu), Rice `` Seeds, Seed coat '', Inondo `` Seeds '', Ibuki-so-sou, Nettle, Ylang-ylang, Iwa-tobacco (Iwabuchi), Iwahiba or Iwamatsu (rolling mushroom), kidney beans, oolong tea, fennel (mustard) , Wheat (Chibusanoki), Ukiyara (Sanryo), Uguisukagura `` Fruit '', Himewikyo, Turmeric (Urban), Duckweed, Usubasaishin or Keirinsaishin or Oshusaishin (spicy), Usvenia Oy, Usbeni Hollyhock, phalaenopsis, weevil (summer hay), udo or shishiudo (gung, alone, tangy), unyadegato (unkari) A, Cat's Claw), Uba tea, Ume (Japanese apricot `` seed, pulp ''), Japanese white beetle, Uwaurushi (Kusako-kemomo), Unshuumika (Chen), Unboku, Estragon, Ezoukogi (Tomogoka), Ezo-suzuran, Echinashi (Hosobamurasaki Barengiku) , Enishida, Enokitake (榎 茸), Ebisugusa or Cassia Tora (Keiko), El Campre, Elderberry “fruit”, Elemi, Elingi or Pre-Lotus Elinji, Enju (Safflower, Camellia rice), Pea, Ogi or Kibanaogi (Yellow Twilight), Naruko Yuri or Kagura-mabanaru Koyuri (Yellow), Ohisasa Yuki or Sasano Yuki, Ouren (Yo Ren), Ogata Houken, Okarasuri (Karokon), Oguruma (Civil Works Incense), Otsu Samurai (Prevention), Psyllium , Car forage), toucan thistle (Zhengzhou leakage, bald eagle), toucan Mi (coconut palm), Toucan cactus, Ovana okera or okera (white moth), Osara squirrel (Banaba), Ooyabaya shabushi "fruit", Oohashigusa (cereal sperm), Omia catechu, Oomihasa or hawthorn (Mountain vegetation), Omiyashi, Omiji Barley), Oka, Okazeri (snake bed), Okra `` fruit '', Oguruma (overturn), Oguri higotai (Zhengzhou leakage, indigo head), Panax ginseng or Tochibaninjin (Ginseng), Hypericum pertussis or Pterotus pertussis or Hypericum perforatum (Brother cut grass), Odorikosou (continuous), Onamomi (Circle ear), Onigomi, Onikoro or Tokoro or Nagadokoro (Hikai), Oninoyara (Tempa), Onyuri or Sasayuri or Hakatayuri (Yuri), Onoelan, Ononis, Halibut楡), Ominae (losing sauce), Yuuko, Dutch mustard (watercress), Ora Dazelli, Dutch beech, Dutch bees, olives `` fruit, seeds, leaves '', oregano, orange `` fruits, peels '', silkworm (ripe yellow), cacao `` fruits, peels, seeds '', oysters (bamboo `` leaves ''), Kagi-Kazura (Katsufuji), Kakidooshi or Kantorisou (Lotus), Kakiran, Gaku Hydrangea, Cassia, Casinoki (fruit berries), Gadju (Kashiwa), Kashiwa (Kashiwagi, Kashiwa), Cascarilla, Cascala Sagrada, gypsophila, gypsophila, cynomolgus (Kinshato), kaniwa, carnation, valerian (valerian), hippopotamus, birch or fern birch (white birch), gerbera, pumpkin, kapokoki `` seed '', kahoku salamander (蜀椒), gama Yellow), chamomile or roman chamomile, chamomile (weed), cam cam (camo duck), collar, chrysanthemum, calla or cinnamon , Oat biscak (half summer), oats, guarana `` seed '', carahoo (kokubaku), karaya, galyu, karin (garden), garcinia, kawamidori, kawara-saiko (reeled rape, white millet), kawajisha, kawaratake, kawaranadeshiko ( (Stone bamboo) or Ezokawaranadeshiko (buckwheat noodles, buckwheat), Chinese carrot (blue potatoes), Chinese mugwort (bamboo chin mackerel), kansui (kansui), licorice (licorice), kantarasa, candelilla, kanto, canna, kanran, raspberry (ezo strawberry) , Dutch Strawberry, Shrimp Strawberry, Nawashiro Strawberry, Momiji Strawberry, European Raspberry Strawberry), Kiwi `` Fruits, Leaves '', Kikarasuuri (Gurone), Chrysanthemum (Kikyo, Kikyo-root), Chrysanthemum (Chrysanthemum, Chrysanthemum, Datura), Kikutanigiku, Kisage (Salmon), Gigigishi (sheep hooves), pheasant (salmon), Kizuta, Kidachi aloe, Kidachi mint , Quina, linden (shinkona, red linden), quinoa (quinoa), yellowfin (yellow tuna), bean, gymnema sylvester, kimenkaku, cabbage, cabbage `` unripe fruit '', caraway, cucumber, gyory (Nishikawa willow, tail willow), Kiraja saponaria, Kiraya, Kiransou (Kinza small grass), Kumquat `` fruit '', Kinkiju, Ginseikai, Kimbusen, Kinma, Kinkihiki (Sentsukusa), Kinlan, Ginlan, Ginryohen, Guapeba Vermela, Guava `` Fruit '', Guayule, Quercus・ Infectria (garlic pods), kukui nuts, kugenumaran, wolfberry (bamboo shoots, coconut palm leaves, cocoon leaves, ground bone skin), cedar casserole (Tenmon winter), kudu (kudzu), camphor tree, gooseberry `` fruit '', ginseng (yellow flower candy) ), Kutiti River, Gardenia (Yamako), Kunigi (Kusou), Kupu , Kubeba, Kumazasa, Kuma-tsutsura (horse whip grass), Clara (bitter), cranberry `` fruit '', chestnut `` seed, fruit, astringent skin '', curcumigo latifolia `` fruit '', grapefruit `` fruit and leaf '', buckthorn, kuroganemochi ( Rescue), Kamehiba Kokoshi or Kurobana Hikikoshi or Hikiokoshi (prolonging grass), Clove (clove, clove), Gumbai Tuna (Sekimei, Sekimeishi), Keigai (Kashiwa, Hakuho), Celosia (Chicken flower, Chicken crown), Gekkavidin, Gecko (Laurel Tree), Kenashi Sartoribaira (Dogo, Mountain Return), Gentiana, Gennoshouko (Old Grass), Kemponashi (Kigushi), Kokisekoku, Kishimizukan (Koji, Tachibana, Obenimikan, Fukurimikan, Sagamikouji, Ponkan, Santara Skin) ), Koshinbara (monthly flower), Koiusui mint, Kozo `` Fruit '', kocha (tea), kohone (river bone), koh hong (Tsubakimoto, Karamotomoto), Kouriang, Kouryokyo (Koryo), cilantro `` fruit '', Koouren (Huo Ren), Koganebana (Yellow Gon), cowberry (Etsu) Tachibana), Coco `` fruit '', Goshuyu (Kurei), Gosho strawberry (Golden bonsai), Pepper (Pepper), Cosmos, Copaiba balsam, Coffee `` Seeds, leaves '', Kobushi or magnolia (hot pepper), Burdock (Beef bowl, Gyudon) ), Coconut lees, sesame (sesame seeds), sesame seeds (genus ginseng), trash (goji), saneka or binankazura or pine bush, wheat (wheat), rice or rice bran `` red potato, white birch '', rice oil, cola acminata `` seed '' , Cola Bella "Seeds", Colony Lilla, Koroha "Fruits", Colombo, Conzurango, Kombu, Konjac, Comfrey (Chang Zhang grass), Sisal (Sisal Asa), Cy Hailan Sakaneran (Ezosakaneran), Sasima button eel or Sina button eel or Cinnamon ginseng (weikensen), Sakura (Ooshima cherry, Yamazakura, Oyamazakura, Edhigashi, Mamezakura, Miyazakura, Someiyoshino, Takanezakura, Kasamikan, Sakura, Sakura, Sakura Fruit, bark (cherry bark) ''), cherry, pomegranate, sasa, sababagin orchid, sasanqua, sardine sardine (salmon), sweet potato, sugar cane, sugar beet, sunflower jujube (saccharin), saffron (bancho flower, west safflower), sapodilla, sapotea mariyo , Pomelo `` fruit '', savonso, salmon berry `` fruit '', salamander (sasma), salamander (sand indigo sashimi), salvia (sage), scallops (bamboo rape), sawagurumi (wild walnuts), sankaku cactus, sangre Degrad ( Loton), Salamander, Sansino Ginseng (37 ginseng), Sanshuyu (Yamamuro), Sansho (Yamamuro), Sandskon (Yamazune), Shea (Kalite), Cyano `` fruit '', Shiitake mushroom (Shitake), Sion (Purple spider), winged bean, jikitarisu, shikunshi (messenger), perilla or ajizo or chimmentiso or catamenzo (purple leaf, shisoko), rosewood, linden tree, red-faced syringae, renilla (ream), shimeji (hinshimeji, shakashimeji, hatake-shimeji (Shimeji, Beechhimeji, Honjimeshi, Shirotamogitake), Shimokuren (hot pepper), Shimosukeso, potato, peonies (shakuyaku), shajin (shasan), jasmine (matsurika), janohige (barley winter), shrimp scorpion, shukukonka miso, Shukushamitsu (sand, crushed sand), Juzdama, Shul "fruit" Oyashi, Ginger (Ginger), Zouzan Hydrangea (Tsuneyama), Shobu (Bamboo, Sone), Shozu "Fruits", Shirakashi "Seeds", Silane (Biacyu), White Rooster "Seeds", White Clover (Clover), White Clover (chicken) Bone grass), White squirrels (Nioiris), Shirobanatsuta "flowers", Shirobana rupin, Shirominanten (Nan Tenshin), Cinchona sylbula, Ginkgo biloba (Mizuka, Mizuka flower, Shencho flower), Cinnamon, Cinnamum cassia (cinnamon), watermelon (Saijo), Honeysuckle (Silver flower, Shinobu), Sorrel (Acami), Swimberry `` Fruit '', Star Apple, Stevia, Strawberry `` Fruit '', Suzu-Psycho (Xucho), Sugina (Question), Suburihiyu (Horse tooth soap, (Horse teeth), plum (fruit), sulgaran (oran), Japanese red pine cone, Japanese larch, Japanese pine Ukizuta, Japanese walnut, Japanese hawthorn, Japanese cypress, Japanese dandelion, Japanese horse chestnut (Maroni), Japanese pear `` fruit '', Japanese cypress, Japanese elder (Elder), Japanese squirrel (Juniper, Juniper), Japanese yarrow (Milfoil), Japanese cypress Roses, pearl butterflies, mistletoe, Atlantic mint or wild mint (Dendrobium, sarcophagus), Cedron (Boshushuboku), Zeniaoi, Hiroha Senega, Senega, Seri, Celery, Senkyu (river cucumber), Senshinren (Pureshinren), Sendan, Se Ninja cactus, senna `` fruit, leaf '', ginseng (daikon), senburi (medication), senboku, soka (grass), elephant clover, soshinwaby, buckwheat `` seed '', potato monogram, broad bean, daiou (daihuang), radish, Soybean, Daidai (orange peel, fruit), Takasago Gurihigotai (southeast indigo sashimi), Takasaburo (Cirrus lotus), Takatodai (Daisatsu), Takaharabi (Cirrus), Dark sweet cherry `` fruit '', hollyhock (Thyme, Hyorika), Tachidokoro (Hikai), Tachiyanagi, Tamarind `` Seeds '', Onion, Tamshiba (hot pepper), Taranoki `` Fruits, leaves, root bark '', Tarayo (Large winter blue, Ichiha tea, bitter tea, bitter tea ), Dahlia (Tenji button), Tarwi, Tankimi, Tanshichiwa, Tanjin, Dandelion, Shirobana Dandelion or Mouko Dandelion, Damma , Cherry "fruit", Imperata "fruit, roots, buds", the district Seth carrot (Takefushi carrots), chicory

, Chancapiedra, Tulip, Chocolate-no-stay, Korean thistle (Artichoke), Korean dragonfly (Dahi), Korean senile (Amber), Korean mugwort (Bamboo leaf), Choreimaitake (Amber), Chorogi, Apricot , Tsukushi Kaneran, Tsukuritake (mushroom), Tsuchitakebi, camellia, camellia, tsumekusa (lacquered weed), Tsuyukusa (duck eel), Tsuruazuki (red bean), Tsurutsuchiakebi, Tsurudokudami (what neck rice cake), Tsuruna (Apricot apricot), Tsurunjin (four leaves) ), Tsuruma, Tsuwabuki, Deco, Teuchigurumi, Duberry `` Fruit '', Tengusa, Tencha (boiled tea), Tendai Yaku (glaze), Toga (winter coconut), Toka Kazura, Capsicum (panyu), Toki (toll), Milky-scented (marigold), Chinese horned beetle, Tounabena (river cut), corn Or corn hair (south cocoon hair), horned potato (Sadako), Torindo or Kyakusen Gindou (dragon gall), Tokusa (wood band), dokudami (ten medicines), Tokon (gush roots), toshi or bean pods `` Bark, leaves, roots ''), ash (crusts), tomatoes, tragacanth, triacanthos `` seed '', eustoma, tormentilla, dronoki, troro-aoi, tongamame, nigerian berry `` fruit '', Chinese yam or yam (mountain medicine), nagii-kada (Butcher Bloom), Naginata Koju, Nazuna, Rapeseed, Natsume or Vertical Beans (Sword Beans), Natsumikan, Jujube (Otsugi), Naniwaibara (Golden Cherry), Navena (Continued), Nameko, Nankin Bean (Peanut), Nanten (Southern Sky) Fruit), Namban Karamushi (Sesame), Niooi Sumire, Nigaki (Nakiki), Nigamugi (Sweetness), Nikuzuku, Kei Is Nikkei or Ceylon Nikkei or Yab Nikkei (Cinnamon bark) or Keishi (Keishi branch), Flying tiger plover, leek (palm), elderberry (bone, `` fruit, flower, stem, leaf ''), garlic (large coral), Nurde (penta), Leek, Nemnoki or Nebu or Nebi or Nemurioki or Digokubana (community), Noazami (Daisetsu), Nobara (Natural), Nozenkazura (Lingen Flower), Nogateto, Yarrow, Nodake (Pre-Hu), Novara, Peach, Pineapple `` Fruit '', Hibiscus (Busouge, Furiinbusou, Roselle), Hailindo, Hauchiwa, Pau Dorse, Hakamakuraboshi (bone fracture), Hakukayumatou, Hakusanchidori, Hakusen (white tinea), Hakuruberry `` fruit '', hakobe (breeding), hazelnut (lion) ), Hasidokoro (roth root), basil, lotus (lotus, lotus meat, lotus), parsley (holland jellies), hadakamugi, ba , Hachiku or Madake (bamboo shoots), patchouli, mint (light load, light load leaf), green peas, pearl barley (Yokuinin), Hanasuge (knowing mother), banana, hana mint, hanabishi (Sitoshiri, Shishitsuri), vanilla bins, papaya, Hakhakusa (鼠 麹, Pahalobobo, hub `` whole plant, stem, leaf '', paprika, scallop, clam or honey beetle (vine lion), hamasuge (kotsuko), kingfish (Shitsu lion), hermanus (maikai blossom), kingfish (Beach windbreak), hamamelis, palm, rose (rose), balata, balata rubber tree, agaric (agaric, white-faced agaric, edulis), halin, parisurus, palietalia, harukanran, balsamina (brurei, nigari), haruni Skin, white skin, green leaves), Barbasco, Harlindow, Handai Kai Sea), breadfruit, giant eagle (dried), chickweed carrot (participant), pecan nuts, ganbanana (stone wall, vine pearl flower), hygurumadaria, higotai, shinki (rhizomi), pistachio, beet, human camellia (stone wall) , Hitosubaenida, Daisies (Daisy), Hinata Taikozuchi (cow knee), chickpeas, cypress, hiba, castor, sunflower, peppers, Himeuzu (Tengu), Himamatsu (Kaori), Himatsutake (Kariharatake, Hiromatsutake), Himetake Pimenta “fruit”, beakshi, beakkyu, jade “fruit”, chickpea, lentil, bellowed oyster, broad-leafed oyster (bald bald eagle), loquat “fruit, leaf, stem”, betel wax (large clothes, coconut), puer tea Tea), Japanese calyx (southern rattan), Japanese cypress, Japanese dandelion (Japanese winter flower, Japanese winter leaf), Japanese basil (Orchid), Japanese bean (Japanese peas), Japanese peach ( , Fruit, pericarp, seeds, leaves, beech, Fuyumushinatsutake (corticus), Brazilian licorice, Brazilian carrot, Brazilian palm, Brazilian elephant, blackcurrant `` fruit '', blackberry , Branjen, plum `` fruit '', fullcellaria, blueberry (Prunus serrata), prunes, floral blanca, blond psyllium, bundo (mung bean), hazelnut, loofah, hetsukaran, safflower (safflower), heneken, belladonna, berry `` fruit '', Percea, Pervianberg, Pereschia grandifolia, red beetle or duckweed (scenic sky), boysenberry `` fruit '', scallop or narcissus or nendo or hahagigi kokia (silk), horsiran, scallop Grass), paw Rear sapota, Pouteria lukuma, cypress, boufou (windproof), spinach, physalis (Torone), honoki (Japanese rustic, magnolia), bokeh (wooden mushrooms), hosoba-a-baku-gaku (painful), hosobaoguruma, hosobanaokera (蒼朮) , Hosoba Kirinso (Keiten 37), Hosobabarengiku, Bodaiju (Bamboo Tree), Button (peony `` flowers, leaves, stems, bark ''), Buttonpi (peony skin), Hop, Jojoba, Borigi (Lulichisha), Bordeaux, White berry `` fruit '', Hononiku (meat jugo, Daiki), Honsekoku (iron crust stone sparrow, sparrow mountain sarcophagus), Mizuru tennansho (Tennan star), Maitake (maiko), maou (mao), maca, macadamia nuts , Margaret (Mokushungiku), Makuri (Marine Grass), Magwa (Mulberry White Bark “Bark, Leaves”), Magnolia Splengeri, Mazaran Uba, Masala Suba de Ceara, Ma Jeira de Boi, Mashua (Tamanozenhalen, Kyukonkinrenka), Matatabi (wooden cocoon), Matsukasa, pine `` leaves, bark, root '', Matta Orillo, Matsuhodo (茯苓), Matiko (Cordon Sillo), Mayoram (Hanahakka), Malvano Jazine (Bitter), Mulberry `` Fruit '', Quince, Malene (Birod mud squid), Mango, Mangosteen, Manzanya (Andesca Mitsule), Manshu gurumi, Mandarin `` Fruit '', Mannentake (Ganoderma), Pheasant (Fruit `` fruit ''), Mishima Saiko (Shibahu), Mizuoobako or Mizuasagao (Dragongrass), Mizokakushi (Half-sided lotus), Misohagi (Chinese cucumber), Michiyanagi or Niwayanagi (Henpotsu), Mitsugashi, Mitsuba, Midori Sapote, Green mint, mimosa, myoga, miracle fruit “fruit”, myrrh, milobaran, wheatwaragiku Mukuge (mushrooms), Mukunoki, Mukuroji (life-prolonging skin), Munya, Murasaki (purple root), Murasakikibu or Omurasakikibu (purple pearl), Murasaki corn, Murasaki Kinafuji (Kunming chicken blood wisteria), Megakansa Opuntica, Mejiki (Masuhagi) , Meowugi, Melaroika, Melissa, Merirot, Melon `` fruit '', Moukomomogi, Mosouchiku, Mokkirin, Mogi (Pepper), Mochinoki, Mokko (woody incense), Japanese peach leaf, peach (peach `` leaves, seeds, flowers, fruit '' ), Palm, Morello Cherry `` Fruit '', Morohaya (Hemp), Yakawa Murasakiimo, Yakuchi (Masami), Cornflower (cornflower), Cornflower, Yacon, Yachabushi (Yagar) or Himeyashabushi or Obayayashabushi `` fruit, peel, Hachiho, Yachiyanagi, Yatsude (octagonal gold plate), Mistletoe (willow infestation), Willow (mosquito) Willow, Twillyanagi, Weeping Willow, Red Willow, Pussy Willow, Incolyana Nagi, Kinuyanagi, Koryanagi, Unryu Willow, Miyamayanagi, Palm, Yamanagi, Obayanagi, Tairakinuyanagi, Foxwood , Yabu tobacco (crane, Tenmeisei), pokeweed (commercial land), Japanese alder (Yamabuchi), Japanese yam (Yamamushi), Japanese mugwort, eucalyptus, Yukinoshita (tiger ear grass), Yucca or flavifolia, Yuzu "fruit", lily , Wormweed, Mugwort (Leaf), Ligan (Raimaru), Lime `` Fruit '', Rye, Lacanca `` Fruit '', Raspberry `` Leaf, Fruit '', Latania (Clamelia), Lackey or Shallot (Lady), Lavender, Longan ), Agave (Aonogave, Fuku (Agave), Ryokucha (green tea), apples `` fruit, seeds, leaves, roots '', gentian, lubas or suabisimus (cool), Ruritama thistle (cold tiger tiger, Xinjiang indigo head), lychee (toothpick, toothpick core), Lettuce (Chisha), red currant `` fruit '', red pitaya, lemon `` fruit '', lemongrass, forsythia or Chinese forsythia (ream), forsythia, wintersweet, wax palm, locon (reed, root), logan berry `` Fruit ", rosemary (mannenrou), rosehip (novara), wasabi, cotton wisteria (Moonjang flower), bitumen (ground).

  As seaweeds, seaweed [Green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usba aonori, Sugiaonori, Hiraaoori, Boaonori, Hosoeda Aonori), Anaaoosa (Aosa)] (Macombu, Resilibu, Hosocome, Mitsuishi Kombu), Wakame, Hirome, Aowakame, Giant Kelp (Macrocystis Integrifolia, Neocitus Ruetkeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa Umiuchiwa, Akaba Umiuchiwa, Konami Uchiwa, Okinawa Uchiwa, Usuki Uchiwa, Etsumi Uchiwa], seaweed [Red algae: Hijirimen, Maca (Tengusa), Hiraksa, Syria, Oaxa Caterpillar, Yatabegusa, Yuukiri, Shimatengusa, Tosakanori, Togekirinsai, Amakusakirinsai, Giraffe, Juniper ginkgo rhinoceros, Tsunomata, Totsuka (Yahazutsu notamata), Ezotsunotamata, Togetu no Takita , Yaleus banori, Kagius banori, Susius banori, Haius banori, Akamo mizunori] and the like.

  In addition, other algae such as green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Anemone: Quanomomo, Volbox: Ougenmawari, Volbox, Palmera, Yotsumeo, Aomido: Chrysanthemum, Aoumidro, Tirguimidro, Hibidoro: Hibidoro, Aosa: Anaaaosa, Amiaosa, Nagaaosa, Kawanori: Kawanori, Friccella, Shiogusa Magama genus: Magamata, Iwagata: Fusaikawa, Surichogata, Heraiwata, Kurokita, Hanemo, Genus: Mill, Chromel, Sakibutimil, Nagamil, Hira Lemon, Casanori genus: Casanori, Jusmo genus: Jujusmo, Tamajusmo, Mizojuzumo, Mika ヅ kimo, Korekaete, Tsutsumimo genus: Kyukougusa, Hitogususa genus: Humanegusa, Hirohatohigususa, Umushihimoe, Sakikimoe Sumiremo: Sumiremo, Hoshimamidro, Fushinashimidoro etc.).

  Cyanobacteria (Suizenjinori: Suizinori, Aoko, Nenju: Kawatake, Ishijelly, Hassai, Yuremo, Rasenmo (Spirulina): Spirulina, Trichodesmium (Iaciasio), etc.

  Brown alga Sanadagusa: Sanadagusa, Fukurimamiji, Commongusa: Commongusa, Yahazugusa: Ezoyahaz, Yahazugusa, Uraboshiyahaz, Jigamusa: Jigmygusa, Umichiwagusa, Umamisakugakua, Hakusou : Navarimo, Chasomen genus: Motsuki Chasomen, Matsumo genus: Matsumo, Nagamatsumo genus: Nagamatsumo, Okinawa mozuku: Okinawa mozuku, fake Tomozuku: Niseft Mozuku, Futomozuku: Futomozuku, Ishimozuku: Ishimozuku, Kuromo: Kuromo, Nisemozuku: Nisemozuku, Mozuku: Mozuku, Ishige: Ishige, Iroro, Ichimegasa: Ichimegasa Umibosu, Urushigusu: Urushigusa, Keurushigusa, Tobaccogusa, Composita: Composita, Haberodomo: Haberodoki, Havanori: Habanori, Habanori: Habanori, Commonbukuro, Ezobukuro, Ezobukuro Chishima fukuronori genus: Chishima fukuronori, Kagomenori genus: Kagomenori, Muraridori genus: Murachidori, shark genus: shark zusa, genus genus: lobster, genus genus: yokojimamanori, Ganoderma genus: cynomolgus, fennel spider: fennel spider, vine genus: turmo, turtle genus: sea turtle, scorpion genus: sujime, misji kumbu genus: misji kumbu, atsuba misji kumbu, kumbu genus: tsutsugara kumbu, oyster kumbu, onikombu, gakonbubu Barracuda, Trollocombu: Trollocombu, Antokume: Antokume, Kajime: Kajime, Tsuruarame, Kurome, Kikuishikombu: Kikuishicombu, Nejikcombu: Nejicombu, Kuroshiome: Kuroshiome, Nekoshikonbu, Aramei Genus: Ainuwakame, Chigaiso, Oniwakame Ezoi Shige: Ezoi Shige, Yabane Moku: Yabane Moku, Rappa Moku: Rappa Moku, Joromoku: Ugano Moku, Jo Lomoku, Hiemoku, Honda Walla: Tamanashimoku, Isomoku, Nagashimamoku, Akamoku, Shidamoku, Honda Walla, Nejimoku, Narasamo, Mametawala, Tatsukuri, Yatsumata Moku, Umitora no Moku, Fushishimoku, Mohmokumoku, Hahamokumoku , Ukimo: Ouokimo, Burukimo: Burukimo, Kayamonori: Kayamonori, etc.).

  Red algae Genus: Sea urchins, Tsukumonori, Anemone crickets, Astragalus, Amaranthus, Amaranthus, Agaricata Genus: Tamaitadaki, Kaikekenori Genus: Kaginori, Kaikekenori, Tengusa: Himetengususa, Hytengususa, Oobusa, Nambugu , Kohira, Yohira, Kinukusa, Hibirodo: Hibirodo, Himebiro, Isomumedo: Isommedo, Aedes: Rhizome, Ryumons: Ryumon, Ranunculaceae, Nisekare Naminohana: Hosobanaminohana, Naminohana, Sangomodoki: Ragaramomodoki, Shiogusagoromo: Shiogusagoromo, Iwanokawa: Etsukiiwanawa, Kinokawa: Kinokawa, Kanonote, Sangori Kyono Shiro, Sakuranori, Nikumukade, Tambanori, Tsurutsuru, Fudarak, Marubahdarak, Isonohana: Nurakusa, Ronulaksa, Oomkadenori, Hirakintoki: Hirakintoki, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Rice, Tokamatsu, Hitomatsu, Fushikintoki, Tsunomude, Nagakinito, Crayfish Nagaobane: Naganobane, Funori genus: Hanafonori, Fukurofunori, Mafunori, Karekigusa: Karekigusa, Tosakamodoki: Hosobanatosakamodoki, Hironotosakamodoki, Yatsudegatatosakamodoki, Kurotosakamodokisane, Zotosofomoza Tosakamodoki, Nankaitosakamodoki, Himetosakamodoki, Kinuhada: Kinuhada, Ezotosaka: Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hana Tacarimenia, Hoonoo: Honoo, Hikagenoit: Hikagenoit, Usuginu, Nikuhounoo: Nikuhounoo, Venus nago, Venus nago, Susca veni: Suscabeni, Okarugusa: Yamadagusa, Mirin: Mirin, Hosokamiso , Isomocca genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagibaranori, Kojinoo: Kizino-o, Isodantsu: Isodantsu, Atsubanori: Atsubanori, Ogonori, Ogonori, Ogori Mizo-okonori, birch, beniogonori, fukureshinori, purple birch, shinkaibanori, spruce birch, kataogonori, ryukyuogono Li, Atlantic scorpion, Ivy genus, Yumigataonori, Kubireogonori, Mosagogonori, Kinubanori, Amanita genus: Hachijotensamodoki, Fushikenori genus: Fushikenori, Namiiwatake, Nymphalanthus Itagususa, Saimi, Hari, Lotus Jigusa: Lotus Jigusa, Sugi nori: Ikanoashi, Hosoibonori, Nobonori, Black Haginansou Genus: gourd, cherries, octopus: octopus, sedge, genus: genus, genus: goat, dals : Dulse, Scorpionus, Anadalus, Weibusa: Uebusca, Benifronori: Benifuronori, Fusunagi: Futsutsunagi, Himefusunagi, Hirohafushitsugi, Otsunagisou, Hirawatagigi, Usuwasugi , Egonori: Egonori, Futogigi, Saeda: Saeda, Chirimomiji: Chirimomiji, Konohori family: Habutaenori, Konohori, Suzuronori, Usubeni: Genus: Kagios banori, Yareus banori, Susius banori, Heius banori, Usubanorimodoki: Usubanorimodoki, Ayanishiki genus: Ayani Genus: Aeginu, Dadia: Enasidadia, Shimadadia: Isohagi, Shimadadia, Dajimodoki: Dajimodoki, Euglena: Morayogusa, Fuyugusa, Makuri: Makri, Yanaginogi, Yuna, Yukinagina , Sasabayanaginori, Sozo genus: Kurosozo, Kobsozo, Hanesozo, Sozonohana, Hanegusa genus: Hananegusa, Hananegusa, Kozanemo genus: Kozanemo, Isomurasaki, Hosozozanemo, Himegoshigusu, Giant genus Isomagusa, Suginasugusa: Suginasusa, Isobashou: Isobasho, Fujimatsumo: Fujimatsumo, Sawweed: Haeshinosakiba, Kawamoku : Kawamoku, Aokawa Mozuku, Himekawa Mozuku, Ideyugogome: Ideyugogome, Okichimozuku: Okimozu, Otosa, Chinomo: Chinomo, Chisujinori, Chisujinori, etc.).

  Axial algae (Shajikumo, Shiratamamo, Hoshitsurimo: Hoshitsurimo, Rikunotumusu, Flaskmo: Himebumo, Chaboflasmo, Tripera, etc.), yellow algae (Hikarimo: Hikarimo, etc.), etc.

  In addition, the materials derived from animal materials include chicken crown extract, cow or pig, human placenta extract, cow or pig stomach, duodenum, intestine, spleen extract or decomposition product thereof, cow or pig brain tissue Extracts, cattle / pig or fish collagen hydrolyzate (acid, alkali, enzyme etc.) degradation products, water-soluble collagen or collagen derivatives such as acylated collagen, cow or pig elastin or elastin hydrolyzate (acid, alkali, Enzymes etc.) or water-soluble elastin derivatives, keratin and its degradation products or their derivatives, silk proteins and their degradation products or their derivatives, porcine or bovine blood cell protein degradation products (globin peptides), cattle or porcine hemoglobin degradation products (Hemin, hematin, heme, protoheme, heme iron, etc.), milk, casein and its degradation products or their derivatives, skim milk powder and their degradation products or their inducements Body, lactoferrin or decomposed product thereof, egg ingredients, fish hydrolyzate, nucleic acid-related substance (ribonucleic acid, deoxyribonucleic acid), or the like.

  In addition, as a material derived from a plant or animal material, any site, cell, tissue, organ, metabolite, etc. derived from a gene recombinant or a cell fusion can be used. Furthermore, for example, an undifferentiated cell group, a cell group in the middle of differentiation, or the like obtained by culturing an arbitrary site, cell, tissue, organ or the like can also be used.

(14) Marine components Seawater such as deep seawater, for example, sea salt, dried seawater, inorganic salt (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud (from the Dead Sea or Atlantic Ocean) Fango), for example, Italian mud, German fungo, Eifelfango, Freiburg fungo, etc. Sea mud or mud from various locations (containing components: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, Magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), saintoku stone, etc.

(15) Microbial culture metabolite Yeast metabolite, yeast extract, bacterial metabolite, bacterial extract, mold or actinomycete metabolite, mold or actinomycete extract, natto metabolite, natto extract, rice fermentation extract , Fermented rice bran (red rice bran, white birch) extract, Euglena extract or decomposition product thereof or water-soluble derivative thereof, trehalose or derivative thereof, lactic acid fermentation product of raw milk or skim milk powder, fermented lactic acid bacteria of legumes, coconut plant Lactic acid bacteria fermented products.

(16) α-hydroxy acids Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(17) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, Bengala, black iron oxide, gunjo, chromium oxide, chromium hydroxide, carbon black, calamine, etc.

(18) UV absorber / blocker benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonate, dihydroxy Dimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), paraaminobenzoic acid derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, Octyl paradimethylaminobenzoate), methoxycinnamate derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, 2-ethoxycinnamic acid cinnamate) Til, sodium paramethoxycinnamate, potassium paramethoxycinnamate, diparamethoxycinnamate mono-2-ethylhexanoate glyceryl, etc.), salicylic acid derivatives (octyl salicylate, phenyl salicylate, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, Methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate, etc.), urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives , Camphor derivative, furan derivative, pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative, vitamin B6 derivative Body, umbelliferone, esculin, benzyl cinnamate, cinoxalate, oxybenzone, dioxybenzone, octabenzone, thrisobenzone, benzoresorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escalol, zinc oxide, talc, kaolin etc.

(19) Whitening agent Paraaminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, camphor derivative, furan derivative, pyrone derivative, Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin C or derivatives thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or derivatives thereof, kojic acid or derivatives thereof, oxybenzone, benzophenone, arbutin, guaiazulene, Shikonin, baicalin, baicalein, berberine, placenta extract, ellagic acid, lucinol, etc.

(20) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or a derivative thereof (hydroquinone benzyl ether, etc.), kojic acid or a derivative thereof, vitamin E or a derivative thereof, N-acetyltyrosine or derivatives thereof, glutathione, hydrogen peroxide, zinc peroxide, placenta extract, ellagic acid, arbutin, lucinol, silk extract, plant extract (camille, mulberry, gardenia, pearl oyster, clam, clam, mugwort, honeysuckle) , Kihada, dokudami, matsuhod, pearl barley, mussel, hops, hawthorn, eucalyptus, yarrow, altea, keihi, mankaishi, hamamelis, yamagwa, lifelong grass, bellflower, toshishi, zetsugo, gyu, mao, senkyu, dod Tsu, Psycho, storm, littoralis, Scutellaria, peony bark, peony, cranesbill, kudzu root, licorice, Fushi aloe, Cimicifuga, safflower, green tea, black tea, Ali elixir), and the like.

(21) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercuric chloride, hydrogen peroxide, zinc peroxide, hydroquinone or its derivative (hydroquinone benzyl ether).

(22) Turnover promoting action / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, ganoderma extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe, Ogon, Japanese horsetail, Gentian, Burdock, Shikon, Carrot, Camelis, Hop, Yokuinin, Odori, Semburi, Japanese cypress, Japanese red snapper, Achacha, Hypericum, Cucumber, Necklace, Rosemary, Parsley).

(23) Astringents Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc paraphenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compounds) and the like.

(24) Active oxygen scavenger
SOD, catalase, glutathione peroxidase and the like.

(25) Antioxidants Vitamin C or salts thereof, stearic acid ester, vitamin E or derivatives thereof, nordihydrogua cetelenic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole , Propyl gallate, sesamol, sesamorin, gossypol, propolis, etc.

(26) Lipid peroxide production inhibitor β-carotene, plant extract (sesame culture cell, hamcha, hypericum, hamamelis, clove, melissa, birch, birch, salvia, rosemary, minamitenji, jitsu, ginkgo, green tea).

(27) Anti-inflammatory agent Ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or its Salt, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract, propolis, etc.

(28) Antibacterial / bactericidal / disinfectant Acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or hydrolyzate thereof, alkyldiaminoethylglycine chloride solution, triclosan, sodium hypochlorite, chloramine T, Sarashi powder, iodine compound, iodoform, sorbic acid or its salt, propionic acid or its salt, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p -Chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, O-phenylphenol, Irgasan CH3565, halocarban, hexachlorophene, chlorohexidine, ester Tanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, 2-phenoxyethanol, 1,2-pentanediol, zinc pyridione, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxyethylene lauryl ether, Polyoxyethylene nonyl phenyl ether, polyoxyethylene octyl phenyl ether, etc.), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyltrimethylammonium bromide, benzalkco chloride) Nium, benzethonium chloride, methyl rosaniline chloride), formaldehyde, hexamine, brilliant green, malachite green, crystal bio Tsu DOO, Jamaru photosensitive No. 101-containing, photosensitizer No. 201, photosensitizer No. 401, N- long chain acyl basic amino acid derivative and its acid shark salts, zinc oxide, hinokitiol, Sophora root, propolis, and the like.

(29) Moisturizer Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or its derivative or chitosan derivative, pyrrolidone carboxylic acid or its salt, lactic acid Sodium, mini Sasanishiki extract, natto metabolite, natto extract, loofah water, birch (white birch) or red pine sap

(30) Elastase activity inhibitor diisopropyl fluorophosphate, plant extract (eg, gongoid, hypericum, clara, mulberry leaves, keihi, genokosho, comfrey, salvia, elderberry, bodaiju, button pie), seaweed extract and the like.

(31) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate, thio Glycolic acid and the like.

(32) Antiandrogens Follicular hormones (estrone, estradiol, ethinyl estradiol, etc.), isoflavones, oxendrone, etc.

(33) Peripheral vascular blood flow promoter Vitamin E or derivatives thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, toki extract, cephalanthin, carpronium chloride, minoxidil and the like.

(34) Stimulants Pepper tincture, nonyl acid vanillamide, cantalis tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(35) Metabolic activator Photosensitive element 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placenta extract, biotin, pentadecanoic acid glyceride and the like.

(36) Antiseborrheic agent Pyridoxine or a derivative thereof, sulfur, vitamin B6 and the like.

(37) Keratolytic agent Resorcin, salicylic acid, lactic acid, urea and the like.

(38) Oxidizing agent Hydrogen peroxide water, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, Sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract, etc.

(39) Hair remover Inorganic reducing agents such as strontium sulfate, sodium sulfide, barium sulfide, calcium sulfide, thioglycolic acid or its salts (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate) Strontium thioglycolate) and the like.

(40) Hair swelling agents such as ethanolamine, urea and guanidine.

(41) Dye agent
5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoaminophenol, N-phenyl acetate Paraphenylenediamine, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylenediamine, paraaminophenol, para Nitroorthophenylenediamine, paraphenylenediamine, paramethylamino Phenol, picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N -Phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diamino sulfate Diphenylamine, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, parasulfate sulfate Methylaminophenol, metaaminosulfate Diol, metaphenylenediamine sulfate, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfone Sodium acid, hematein, etc.

(42) Fragrance Natural animal fragrance such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil, Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petit gren essential oil, bay essential oil, vetiva essential oil, bergamot Spirit , Peru balsam essential oil, boad rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essential oil, and other synthetic flavors Fragrance etc.

(43) Dye and Colorant Red Cabbage Dye, Red Rice Dye, Akane Dye, Anato Dye, Ikumi Dye, Turmeric Dye, Enju Dye, Krill Dye, Amber Dye, Caramel, Gold, Silver, Gardenia Dye, Corn Dye, Onion Dye , Tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin etc.

(44) Sweeteners Sugar, sweet tea, fructose, arabinose, galactose, xylose, stevia, mannose, maltose, honey, glucose, miraculin, monelin, licorice extract and the like.

(45) Nutrient enhancer Shell calcined calcium, cyanocobalamin, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron and the like.

(46) Dairy products Milk, cheese, fresh cream, butter, margarine, powdered milk, whey, condensed milk, etc.

(47) Others Hormones, sequestering agents, pH adjusters, chelating agents, antiseptic and antifungal agents, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their degradation products, animal and vegetable polysaccharides And its degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents, anti-inflammatory agents, anti-allergic agents, wound treatment agents, foam increasing agents, thickeners, enzymes, purified water (electronic Water, small clustering, etc.), oral preparations, deodorants / deodorants, bittering agents, seasonings, etc. are raised, and in combination with these, the effects of the present invention or other cosmetic effects are added Or synergistic improvement can be expected

  Hereinafter, production examples, test examples, and formulation examples will be described. However, the present invention is not limited to these examples.

(Production Example 1)
The dried product of bilberry leaves (100 g) is pulverized, heated and extracted with purified water (1.0 kg) at a temperature of about 30 ° C. for about 5 hours, and filtered to obtain an extract (dry solid content: about 1. 0 to 4.0%) was obtained about 1.0 kg.

(Production Example 2)
The dried bilberry leaves were pulverized, and 100 g each was immersed in a 50% ethanol solution, extracted at room temperature for 5 days and nights, and then filtered to obtain an extract (dry solid content: about 1.0 to 4.0%). About 1.0 kg of was obtained.

(Test) Cell growth inhibition inhibition test by blue light The inventor conducted a fibroblast growth inhibition inhibition test by blue light and evaluated the effect of the bilberry extract.

"Test method and evaluation method 1"
A 96-well microplate is seeded with 1 × 10 ⁴ fibroblasts / well. As the medium, DMEM (Kurabo) supplemented with 5% FBS was used. After 24 hours, the medium is removed and replaced with PBS. Blue light region light was repeatedly irradiated using SUNRISE-BASIC TECAN (Tecan Japan). At that time, it was possible to irradiate a specific wavelength by scanning 400 times at 400 nm and 450 nm, respectively. As a control, an unirradiated group was provided, and after replacing with PBS in the same manner, it was allowed to stand for the same time under light shielding. Immediately after irradiation, PBS was removed, and DMEM containing 5% FBS and the bilberry extract or control solvent (50% butylene glycol aqueous solution) prepared in the Examples were added to a concentration of 0.5%. Similarly, as a comparison target, a group to which a raspberry extract and a persimmon extract extracted with known fibroblast proliferation action were added. Then, after culturing for 24 hours at 37 ° C. and 5% CO ₂ , the absorbance was measured using the WST method (450 nm). The specific activity was calculated and shown when the absorbance of the group not irradiated with blue light was 100.

"Test method and evaluation method 2"
By the same operation as in the test method and evaluation method 1, the effect of repeated irradiation of blue light was examined. After irradiating with blue light, bilberry extract or control solvent was added and cultured for 24 hours. Thereafter, the medium was removed as in the previous day, and after irradiating with blue light, the bilberry extract or the control solvent was added and further cultured for 24 hours. Thereafter, the same operation was repeated. After the first blue light irradiation, the cell number was measured by absorbance at 24 hours, 48 hours, and 72 hours.

(Test results)
As a result, as shown in FIG. 1 and FIG. 2, it was confirmed that the bilberry extract of the present invention has an action of suppressing cell growth inhibition by blue light.

Safety test (primary skin irritation test)
The bilberry extract of the present invention was prepared in purified water so that the dry solid content concentration was 1.0% by weight, and Japanese white rabbits (shaved back) (female, 3 per group, body weight around 2.2 kg) ) Applied to the skin. Evaluation was performed using erythema and edema as an index based on the criteria for Draise 24, 48, and 72 hours after application. As a result, in all animals, no erythema and edema were observed, and it was judged that there was no problem with primary skin irritation.

(Cumulative skin irritation test)
The skin of Hartley guinea pigs (female, 3 per group, body weight around 330 g) prepared with purified water so that the dry solid content concentration is 1.0% by weight and shaved on the back. 0.5 mL / animal was applied once a day, 5 times a week. Application was performed over 2 weeks and shaving was performed on the last application day of each week. Evaluation was performed using erythema and edema as indices based on the criteria for Draise on the day after each application and the day after the last application. As a result, in all animals, no erythema and edema were observed for 2 weeks, and it was judged that there was no problem with respect to cumulative skin irritation.

(Acute toxicity test)
The dried bilberry extract of the present invention was orally administered at a dose of 2,000 mg / kg to ddy mice (male and female, 5 per group, 5 weeks old) fasted for 4 hours before the test. The degree and the like were observed over time. As a result, no abnormality was observed in all mice for 14 days, and no abnormalities were found in the anatomy. Therefore, LD50 was 2,000 mg / kg or more, which was judged to be safe.

Production examples of various dosage forms of foods and drinks containing the bilberry extract of the present invention (tablet type confectionery) (parts by weight)
1. Powdered sugar 87
2. Lactose 2
3. Vitamin C 3
4). Citric acid 1.5
5. Gelatin 1% aqueous solution 4
6). Bilberry extract (powder) 1
7). Sucrose fatty acid ester 1
8). Powdered strawberry flavor 0.5
Were mixed uniformly and tableted according to a normal tablet production method to obtain a tablet-type confectionery.

(Tablet) (Part by weight)
1. Cornstarch 30
2. Microcrystalline cellulose 30
3. Carboxymethylcellulose calcium 24
4). Polyvinylpyrrolidone 10
5. Bilberry extract (powder) 1
6). Talc 5
Were mixed uniformly and tableted according to a normal tablet production method to obtain a tablet.

(Capsule tablets) (Weight)
1. Cornstarch 100mg
2. Lactose 100mg
3. Bilberry extract (powder) 2mg
4). Magnesium stearate 2mg
Were mixed uniformly and filled into gelatin capsules in accordance with a conventional method for producing capsules to obtain capsules.

(Tablet health food) (Parts by weight)
1. Salmon nasal cartilage extract containing proteoglycan 4
(Proteoglycan F, manufactured by Ichimaru Falcos)
2. Chondroitin sulfate 2
3. Hyaluronic acid 0.5
4). N-acetylglucosamine 40
5. Collagen extract powder 7.5
6). Bilberry extract (powder) 1
7). Crystalline cellulose 10
8). Sucrose fatty acid ester 3
9. Fine silicon oxide 2
10. Maltitol 30
Were mixed uniformly and tableted according to a normal tablet production method to obtain a tablet-type health food.

(Cookie confectionery) (Parts by weight)
1. Light flour 35
2. Whole egg 10
3. Bilberry extract (paste) 1
4). Butter 20
5. Sugar 13
6). Salt 10
7). Baking powder 0.3
8). Water 10.7
Were uniformly mixed and the cookies were baked according to a conventional method.

(飴) (Weight)
1. Minamata 500g
2. 500g sugar
Is mixed and heated at 120 ° C. under reduced pressure (−500 mmHg), and then cooled to 90 ° C. to this,
3. Bilberry extract (concentrate) 5g
4). 30g of kombu pulverized powder
5. Stevia extract 20g
6). Citric acid 10g
7). Fragrance 1.5mL
Was added and kneaded sufficiently, and this was punched and molded into a circle having a diameter of 15 mm and a thickness of 8 mm to produce a bag.

(Tomato juice drink) (Weight)
1. Commercial tomato juice 1kg
2. Bilberry extract (powder) 3g
Were mixed uniformly to obtain a tomato juice drink.

(Vegetable juice drink) (weight)
1. 1kg of commercially available vegetable juice
2. Bilberry extract (powder) 3g
Were mixed uniformly to obtain a vegetable juice drink.

(Health-oriented beverage) (Weight)
1. Bilberry extract (powder) 0.2g
2. Erythritol 50g
3. Vitamin C 1g
4). Artificial sweetener 0.06g
5. Fragrance 1.2g
The solution was stirred and dissolved in 1 L of water at a liquid temperature of 70 ° C., adjusted to pH 3.0 with citric acid, sterilized using an ultrafilter, filled into a bottle, sterilized with a pasteurizer to produce a beverage.

(Soft drink) (Part by weight)
1. Fructose dextrose solution 20.0
2. Liquid yogurt 30.0
2. Emulsifier 0.5
3. Sodium hyaluronate (molecular weight 50,000-150,000) 0.1
4). Bilberry extract (solid content 1.0%) 1.0
5. Perfume appropriate amount 5. The remaining amount of purified water 100 was uniformly mixed, and a soft drink was obtained according to a conventional method.

Example of preparation of external preparation containing bilberry extract of the present invention (whitening lotion) (parts by weight)
1. Sorbit 2
2. 1,3-butylene glycol 5
3. Polyoxyethylene oleyl ether (25E.O.) 2
4). Ethanol 20
5. Ascorbic acid-2-glucoside 0.3
6). Bilberry extract (1.0% solids) 3
7). 30% green tea-ethanol extract (solid content 1.0%) 8
8). 8. pH adjuster Suitable amount to make pH 5-6 Preservative (phenoxyethanol) Appropriate amount10. Residue with 100 purified water

(Emollient emulsion) (parts by weight)
1. Squalane 3
2. Vaseline 1
3. Stearyl alcohol 0.3
4). Sorbitan monostearate 1.5
5. Polyoxyethylene (20) sorbitan monooleate 3
6.1,3-Butylene glycol 10
7). Bilberry extract (solid content 2.0%) 2
8). Hydrolyzed collagen 1% aqueous solution 1.0
(Ichimaru Falcos, Falconix CTP)
9. Chamomile flower head 70% -1,3-butylene glycol extract (solid content 0.8%) 5
10. Proteoglycan solution 0.2
(Ichimaru Falcos Proteoglycan IPC)
11. Sodium hyaluronate 0.0005
12 Preservative (methylparaben) appropriate amount13. Residue with 100 purified water

(Hair shampoo) (parts by weight)
1. Lauryl sulfate triethanolamine 5
2. Sodium polyoxyethylene lauryl ether sulfate 12
3. 1,3-butylene glycol 4
4). Lauric acid diethanolamide 2
5. 2-Phenylchromone 0.01
6). Bilberry extract (solid content 2.0%) 5
7). Arginine or its salt 0.2
8). Preservative (ethyl paraben) Appropriate amount 9. Residue with 100 purified water

(Granule bath preparation) (Parts by weight)
1. Sodium bicarbonate 60
2. Anhydrous sodium sulfate 32
3. Borax 5
4). Bilberry extract (powder) 3
Were mixed uniformly to obtain a granule bath preparation.

  The present invention contains a cell growth inhibition inhibitor comprising a specific plant extract (bilberry extract) as an active ingredient, a skin aging prevention / amelioration agent based on a cell growth inhibition inhibitory effect, and the cell growth inhibition inhibitor. Various compositions (pharmaceutical composition, food / beverage product composition, cosmetic composition) are provided.

Claims (5)

  An inhibitor of cell growth inhibition by blue light comprising bilberry extract as an active ingredient.   A preventive / ameliorating agent for skin aging comprising the inhibitor of cell growth inhibition by blue light according to claim 1.   A pharmaceutical composition comprising the inhibitor of cell growth inhibition by blue light according to claim 1.   A food / beverage composition comprising the inhibitor of cell growth inhibition by blue light according to claim 1.   A cosmetic composition comprising the cell growth inhibition inhibitor by blue light according to claim 1.

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