JP2014131961A - Animal nutrient composition - Google Patents

Abstract

An animal nutrition composition having an anti-fatigue action and an endurance enhancing action is provided.
A nutritional composition for animals having an anti-fatigue action or an endurance enhancing action by using a composition containing coenzyme Q10, a seaweed extract and a branched chain amino acid.
[Selection figure] None

Description

The present invention relates to a nutritional composition for animals containing coenzyme Q10, a seaweed extract and a branched chain amino acid. In particular, it relates to the use for race animals.

In recent years, the improvement of the racing ability of race animals has reached the limit depending on the breed improvement, and it has become important to improve the ability by improving feed and training methods. What has an effect, such as an anti-fatigue and / or endurance enhancement, is calculated | required as a nutrient component.

Coenzyme Q10 (hereinafter referred to as CoQ10) is a physiological component that is widely distributed in the living world and exists as a component of the mitochondrial electron transport system in cells in the living body. CoQ10 is a benzoquinone derivative, and plays a role as a transmission component in the electron transfer system by repeating oxidation and reduction in a living body. Its usefulness is widely known since it exhibits energy production and antioxidant activity in vivo. For example, it is used for pharmaceutical use as a congestive heart failure drug. Other than medical use, as with vitamins, nutritional supplements, nutritional supplements, senile diseases such as dementia, usefulness for allergic diseases, and increased exercise capacity have been reported, and their safety It can be said that it is a useful means of nutrition because it is high.
On the other hand, CoQ10 is fat-soluble, difficult to mix with water, and difficult to make a uniform solution. In addition, low dispersibility in water leads to low absorbency, and it is difficult to exert an effect.
Furthermore, due to the poor dispersibility in water, if it is packed in a container such as a syringe, it will cause separation and clogging.

Fluorotannin is known to have an antioxidant effect, a vasodilatory effect, an anti-inflammatory effect and a preventive effect for heart disease. Phlorotannins with a vasodilator effect significantly function to promote blood circulation, especially during intense exercise (Patent Documents 1 and 2).

There are 9 essential amino acids that cannot be synthesized in the body among the 20 types of amino acids that constitute proteins. Among them, amino acids having a branched structure include valine, leucine, and isoleucine. These branched chain amino acids are the only essential amino acids that provide muscle energy. It is an energy source that can be used efficiently when exercising. In addition, this branched chain amino acid has a function of suppressing proteolysis in muscle during intense exercise and is a very useful component for exercise.

So far, there have been known fatigue improvers containing CoQ10 and carnitine (Patent Document 3), nutritional supplements (Patent Document 4) containing CoQ10, cyanocobalamin, folic acid, and pyridoxine hydrochloride. Is not clearly described about the fatigue improving effect or endurance enhancing effect. At present, none of the nutritional components aims to increase anti-fatigue and / or endurance, and supports the effects.
From such a background, development of a container that can reliably supply a novel anti-fatigue agent and / or endurance enhancer is desired.

Japanese Patent Laid-Open No. 7-330584 JP 2003-169633 A JP 2006-328010 A JP 2002-212095 A

An object of the present invention is to provide an animal nutrition composition having an anti-fatigue action and an endurance enhancing action.

As a result of intensive studies to solve the above problems, the present inventor has found that the following items can be solved.
[1] A nutritional composition for animals containing coenzyme Q10, a seaweed extract and a branched chain amino acid.
[2] The animal nutrition composition according to [1], wherein the weight ratio is Coenzyme Q10: Seaweed extract: Branched chain amino acid = 1: 0.2 to 0.8: 0.1 to 0.5.
[3] The animal nutrition composition according to [2], wherein the coenzyme Q10 is a mixture with starch particles or soybean protein.
[4] The animal nutrition composition according to [3], wherein the seaweed extract is extracted from brown algae.
[5] The animal nutrition composition according to [4], wherein the branched chain amino acid is valine, leucine or isoleucine.
[6] The animal nutrition composition according to [5], wherein the brown algae is black, arame, or scabbard.
[7] The nutritional composition for animals according to [6], wherein the brown algae contains phlorotannin.
[8] The animal nutrition composition according to any one of [1] to [7], comprising glucosamine, methylsulfonylmethane, chondroitin sulfate, or lactobionic acid.
[9] The nutritional composition for animals according to any one of [1] to [8], which contains wild watermelon fruit juice.
[10] The animal nutrition composition according to any one of [5] to [9], which is for a racehorse.
[11] Animal nutrition according to any one of [6] to [10] in the form of powder, powder, granule, capsule, tablet, suspension, suppository, cream, inhalant or injection Composition.
[12] An animal feed container filled with the nutritional composition according to [6] to [11].
[13] The animal feed container according to [12], wherein the container has a syringe shape.

According to the present invention, a nutritional composition for animals having high absorbability, uniformly dispersed, anti-fatigue action and endurance enhancing action, and a method for producing the composition and a container capable of reliably supplying the composition are provided. Can do.

treadmill Syringe

CoQ10 of the present invention may be reduced or oxidized. Oxidized CoQ10 is a compound represented by the general formula (1).

If CoQ10 used in the present invention is CoQ10 that can be taken or edible as a medicine or food, its origin and production method are not limited. A commercially available product may be used, or a compound that converts to CoQ10 in vivo.

CoQ10 is a lipophilic solid having a low melting point, and is known to have low absorbability in oral administration due to poor solubility in water. For this reason, it is necessary to improve the stability of properties such as the dispersion stability of CoQ10 and the ability to prevent crystal precipitation and the bioabsorbability.

In this invention, it is preferable to mix | blend CoQ10 with animal protein, vegetable protein, or these hydrolysates. Examples of animal proteins include chicken egg protein, milk protein, meat protein, and the like. Examples of plant proteins include soy protein or soy peptide. More preferably, CoQ10 is preferably blended with soy protein or the hydrolyzate. The blending ratio of soybean protein and its hydrolyzate is 5 to 99.9%.

In order to improve bioabsorbability, it is preferable to adsorb and disperse the starch particles. The amount of starch particles is 5 to 99.9%. As an improvement in dispersibility, cyclodextrin inclusion or emulsification treatment is also possible.

When used as an emulsified composition, for example, is CoQ10 dissolved in a sucrose fatty acid ester such as sucrose acetate isobutyrate or a medium chain fatty acid ester as an oil phase component and emulsified using a polyhydric alcohol and an emulsifier? CoQ10 in a water-soluble substance such as gum arabic, agar, water-soluble corn fiber, starch, gelatin, xanthan gum, casein, dextrin, carmelol sodium, polyvinylpyrrolidone in the presence or absence of additives such as organic acids Can be dispersed and emulsified.

The seaweed extract used in this patent is extracted from brown algae and consists of a component containing polyphenol phlorotannin. Specific examples of brown algae include chrome, arame, and scabbard. You may use what is marketed.

If the branched chain amino acid used for this invention is an amino acid which can be taken or edible as a pharmaceutical or a foodstuff, the origin and manufacturing method will not be limited at all. A commercially available product may be used. Specific examples of branched chain amino acids include valine, leucine, and isoleucine.

Assuming that CoQ10 is 1, the blending ratio is 0.2 to 0.8 for seaweed extract and 0.1 to 0.5 for branched chain amino acids by weight.

Joint strengthening can also be achieved by containing glucosamine, methylsulfonylmethane, chondroitin sulfate or lactobionic acid. Moreover, it is preferable to mix | blend citrulline known as a fatigue recovery component. It can be added as wild watermelon juice containing a large amount of citrulline.

Various nutrients, various vitamins (vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin E, etc.), various minerals, dietary fiber, polyunsaturated fatty acids, other nutrients as needed Carotenoids such as carotene, lycopene, astaxanthin, carnitine, sesamin), stabilizers such as dispersants, emulsifiers, sweeteners, taste ingredients (citric acid, malic acid, etc.), flavors, royal jelly, propolis, agarics, etc. Can do. You may mix herbs such as peppermint, bergamot, camomile meal, lavender.

The animal nutrition composition of the present invention includes powders, powders, granules, soft capsules, hard capsules, tablets, tablets, chewable tablets, quick disintegrating tablets, syrups, liquids, suspensions, suppositories, ointments, creams. , Gels, adhesives, inhalants, injections and the like.

Additives that can be used for formulation include animal oils such as soybean oil, safflower oil, olive oil, germ oil, sunflower oil, beef tallow, sardine oil, polyethylene glycol, propylene glycol, glycerin, sorbitol, etc. Surfactant such as polyhydric alcohol, sorbitan fatty acid ester, sucrose fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, purified water, lactose, starch, crystalline cellulose, D-mannitol, lecithin, gum arabic, sorbitol solution, sugar Examples include excipients such as liquids, sweeteners, colorants, pH adjusters, and fragrances. The liquid preparation may be dissolved or suspended in water or other appropriate medium when taken. Tablets and granules may be coated by a known method.

In order to give the animal nutritional composition of the present invention to an animal, it is preferable to fill the container with the composition. When filling, it is preferable that the composition is shaped to match the container.
The container used in the present invention is not required to leak the contents to the outside, and preferably has a shape that is squeezed or pushed out by human power when given to an animal such as a horse. In particular, it is preferable to use a syringe-shaped container shown in FIG. More specifically, a syringe-shaped container having a cap 1 at the syringe outlet, a protrusion 2 matched to the outlet shape at the push-out portion of the syringe, and a protrusion 3 on the outer tube portion so that the push-out portion does not come out. preferable. With the cap structure of 1, the leakage from the container can be prevented. The protrusion 2 can be taken out without leaving the contents of the syringe. The protrusion 3 has a structure that prevents the cylinder portion from being directed out of the syringe, and has an effect of preventing an accident due to detachment during transportation and use.

The syringe can be used in an amount of 0.1 to 2000 ml, preferably 25 to 100 ml, depending on the purpose. The material of the container is not particularly limited and can be used. Metal, glass, plastic and the like can be used, and these can be used alone or in accordance with the place.

EXAMPLES Hereinafter, although this invention is demonstrated in more detail with an Example and a comparative example, this invention is not limited only to these examples.
<Preparation of composition>
Example 1
80 g of CoQ10 powder (Mitsubishi Gas Chemical Co., Ltd.) and 20 g of soy protein hydrolyzate (Nisshin Oilio Co., Ltd.) were placed in a plastic bag and mixed. This is designated as water-soluble CoQ10 (soy protein).
<Drink agent>
The following composition 100g was mix | blended by the normal method, and the uniform solution A was adjusted.
Water-soluble CoQ10 (soy protein) 5g
Glucosamine 5g
Methylsulfonylmethane 3g
Chondroitin sulfate 0.5g
Hyaluronic acid 0.2g
Lactobionic acid 2g
Seaweed extract (cyanol) 2g
Citric acid 0.5g
Leucine 0.5g
Isoleucine 0.3g
Valine 0.3g
NaCl 0.5g
Vitamin E 5g
20g of granulated sugar
Wild watermelon juice 50.2g
Water balance

Example 2
40 g of CoQ10 powder (manufactured by Mitsubishi Gas Chemical Co., Inc.), 40 g of starch particles, 15 g of dextrin, and 5 g of glycerin were put in a homogenizer and heated to 60 ° C. to carry out an emulsification treatment. This is designated as water-soluble CoQ10 (starch).
As a result of blending 100 g of the following composition by a usual method, a uniform solution B could be prepared.
<Drink agent>
Water-soluble CoQ10 (starch) 10g
Glucosamine 5g
Methylsulfonylmethane 3g
Chondroitin sulfate 0.5g
Hyaluronic acid 0.2g
Lactobionic acid 2g
Seaweed extract (cyanol) 2g
Citric acid 0.5g
Leucine 0.5g
Isoleucine 0.3g
Valine 0.3g
NaCl 0.5g
Vitamin E 5g
20g of granulated sugar
Wild watermelon juice 50.2g
Water balance

Example 3
When CoQ10 powder (manufactured by Mitsubishi Gas Chemical Co., Inc.) was used and 100 g of the following composition was prepared, CoQ10 was separated and did not form a uniform solution.
<Drink agent>
CoQ10 4g
Glucosamine 5g
Methylsulfonylmethane 3g
Chondroitin sulfate 0.5g
Hyaluronic acid 0.2g
Lactobionic acid 2g
Seaweed extract (cyanol) 2g
Citric acid 0.5g
Leucine 0.5g
Isoleucine 0.3g
Valine 0.3g
NaCl 0.5g
Vitamin E 5g
20g of granulated sugar
Wild watermelon juice 50.2g
Water balance

Example 4
Powders could be produced by lyophilizing the solutions prepared in Examples 1 and 2.

Example 5
A uniform solution could be made by re-adding water to the powder obtained in Example 4.

Example 6
Using protein as a thickener, 100 g of the following composition was blended by a usual method to prepare a uniform solution C.
Water-soluble CoQ10 (soy protein) 25g
4g protein
Glucosamine 5g
Methylsulfonylmethane 3g
Chondroitin sulfate 0.5g
Hyaluronic acid 0.2g
Lactobionic acid 2g
Seaweed extract (cyanol) 2g
Citric acid 0.5g
Leucine 0.5g
Isoleucine 0.3g
Valine 0.3g
NaCl 0.5g
Vitamin E 3g
20g of granulated sugar
Wild watermelon juice 33.2g
Water balance

Example 7
Using water-soluble CoQ10 (starch), 100 g of the following composition was blended by a usual method. As a result, a uniform solution D could be prepared.
Water-soluble CoQ10 (starch) 35g
Glucosamine 5g
Methylsulfonylmethane 3g
Chondroitin sulfate 0.5g
Hyaluronic acid 0.2g
Lactobionic acid 2g
Seaweed extract (cyanol) 2g
Citric acid 0.5g
Leucine 0.5g
Isoleucine 0.3g
Valine 0.3g
NaCl 0.5g
Vitamin E 3g
Granulated sugar 15g
Wild watermelon juice 32.2g
Water balance

Comparative Example 1
When CoQ10 powder (manufactured by Mitsubishi Gas Chemical Co., Inc.) was used and 100 g of the following composition was prepared, CoQ10 was separated and did not form a uniform solution.
CoQ10 35g
Glucosamine 5g
Methylsulfonylmethane 3g
Chondroitin sulfate 0.5g
Hyaluronic acid 0.2g
Lactobionic acid 2g
Seaweed extract (cyanol) 2g
Citric acid 0.5g
Leucine 0.5g
Isoleucine 0.3g
Valine 0.3g
NaCl 0.5g
Vitamin E 3g
Granulated sugar 15g
Wild watermelon juice 32.2g
Water balance

Bioabsorbability test feeding method For 8-week-old SD rats (male, 3 animals per group, body weight 250-300 g), after orally forcibly ingesting 100 mg / kg rat weight as CoQ10 amount, Three hours after administration, blood was collected from the tail vein using a heparin-added vacuum blood collection tube to separate plasma.

Measurement of the amount of CoQ10 in the blood 1 ml of the obtained plasma is collected, and 2.5 ml of 2% FeCl3-ethanol solution is added and mixed so that the total amount of CoQ10 in the plasma becomes oxidized, and the reduced CoQ10 is oxidized. Converted to type CoQ10. Next, 5 ml of hexane was added and mixed vigorously to extract oxidized CoQ10 in plasma, followed by centrifugation, and the hexane layer was collected in another test tube. 15 ml of hexane extract obtained by repeating this operation three times was concentrated under reduced pressure (10 to 100 torr) with an evaporator, and then 0.2 ml of isopropanol was added and dissolved. 0.2 ml of the obtained isopropanol solution was analyzed by HPLC under the following conditions, and the concentration in 1 ml of plasma was calculated. The results are shown in Table 1.
HPLC analysis conditions:
Column: YMC PACK ODS-A, mobile phase: methanol: hexane = 4: 1 (V: V), detection wavelength: 275 nm, flow rate: 1.0 ml / min, column temperature 30 ° C.
Table 1 below shows the results of the absorptivity test in rats for each of the water-soluble CoQ10 prepared in Examples 1 and 2 and normal CoQ10 (average value of two animals).
It can be seen that the bioabsorbability is improved by using the water-soluble CoQ10 of the present invention.

Endurance test method
SPF-ICR mouse male 6 weeks old (purchased from Charles River Japan Co., Ltd.) was used. The mice were reared in a breeding room set at room temperature: 23 ± 2 ° C., humidity: 55 ± 6%, lighting for 12 hours, and lighting off for 12 hours until the test was completed after arrival. Three cages per cage are housed in a polycarbonate case (Toyo Riko Co., Ltd.) with a floor covering for laboratory animals (White Flakes, Nippon Charles River Co., Ltd.), and a steel stand (Nihon Cage Co., Ltd.). Arranged above and reared. During the test period, mouse solid feed MF (Oriental Yeast Co., Ltd.) and tap water were freely ingested. During the 1 week pre-breeding period, use the treadmill (multiple treadmill system TMS-4, Merquest) shown in Fig. 1 for preliminary running, and divide the groups so that the weight and running ability are equivalent to each group. And 4 groups were prepared with 3 animals per group.

The following samples were administered by oral gavage continuously for 3 weeks so that CoQ10 was 100 mg / kg mouse dose. (However, it is a rest on Saturdays and Sundays.) Three mice were used per sample. Water and horse drink were administered as they were, and water-soluble CoQ10 (soy protein) and CoQ10 were orally administered after being dispersed in soybean oil.
<sample>
1. Water (administered as is)
2. Drink preparation prepared in Example 2 (administered as is)

Endurance measurement was performed 30 minutes after oral administration, and was performed before administration, on the fifth day of administration, on the 12th day of administration, and on the 19th day of administration. Endurance is measured using a treadmill and forced running (inclination 10 degrees, speed 20 m / min). After a while, the mouse gets tired and rides on the stimulation grid from the treadmill. Usually, the mouse escaped from the stimulation grid, but after forced running, the time from fatigue to fatigue from stopping on the stimulation grid for 1 minute was measured.

The mouse that took the drink prepared in Example 2 with respect to the mouse that took Sample 1 was:
The time to stop running from the stimulation grid for 1 minute has been increased from 1.5 to 5 times. From this, it was found that the drink using Q10 can enhance endurance.

Syringe Extrusion Test Examples 11 to 13 Comparative Example 1-1
For each content placed in a syringe made of plastic with an internal volume of 50 ml in the shape of FIG. 2, whether the syringe cap is removed and whether or not to drain for 10 seconds with the outlet directly below, and pressure is applied to the piston, A test was conducted to determine whether or not the entire amount could be smoothly extruded in 2 to 10 seconds. The results are shown in Table 2.

The animal nutrition composition of the present invention is particularly effective as a feed for animals for racing. Examples of competing animals include horses, dogs, chickens, pigeons, camels and the like, which can be suitably used in the field of horses.

1 Cap 2 Protrusion 2
3 Protrusion 3

Claims (13)

Animal nutritional composition containing coenzyme Q10, seaweed extract and branched chain amino acid. The nutritional composition for animals according to claim 1, wherein the weight ratio is Coenzyme Q10: Seaweed extract: Branched chain amino acid = 1: 0.2 to 0.8: 0.1 to 0.5. The animal nutrition composition according to claim 2, wherein the coenzyme Q10 is a mixture of starch particles or soybean protein. The animal nutrition composition according to claim 3, wherein the seaweed extract is extracted from brown algae. The nutritional composition for animals according to claim 4, wherein the branched chain amino acid is valine, leucine or isoleucine. 6. The nutritional composition for animals according to claim 5, wherein the brown algae is chrome, alame or scab. The animal nutrition composition according to claim 6, wherein the brown algae contains phlorotannin. The nutritional composition for animals according to any one of claims 1 to 7, comprising glucosamine, methylsulfonylmethane, chondroitin sulfate or lactobionic acid. The animal nutrition composition according to any one of claims 1 to 8, comprising citrulline. The animal nutrition composition according to any one of claims 5 to 9, which is for a racehorse. The animal nutrition composition according to any one of claims 6 to 10, which is in the form of powder, powder, granule, capsule, tablet, suspension, suppository, cream, inhalant or injection. An animal feed container filled with the nutritional composition according to any one of claims 6 to 11. The animal feed container according to claim 12, wherein the container has a syringe shape.