JP2013116080A - Fluidity lactic acid feed for rumination livestock and manufacturing method of the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To manufacture a fluidity lactic acid feed for rumination livestock in which a dry powder main raw material in which an organic substance generated from a domestic production agriculture system and an organic substance of a part of waste are performed by grinding/purifying by machining processing is added with a plant cellulolytic enzyme cellulose and a hydrolysis water and lactic fermentation water, an enzymatic saccharification fluidity main raw material after hydrolysis is adjustment-mixed with a homotype lactobacillus and sugar, then is performed with lactic acid fermentation to have the range of pH 4-4.5.SOLUTION: The manufacturing method of a fluidity lactic acid feed for rumination livestock is characterized as follows. A dry powder main raw material in which an organic resource generated from a domestic agriculture system, an organic substance of a part waste, and a dry material crude feed raw material of a grain residue, rice straw, straw, forage rice plant, and grass or the like are performed by grinding/purifying by machining processing to a granule having 1.5-500 micrometer of a granule distribution to be easy to perform enzymolysis and lactic acid fermentation is added with a plant cellulolytic enzyme cellulose and moisture by a sealed container, the enzymatic saccharification fluidity main raw material after hydrolysis is adjustment-mixed with a homotype lactobacillus and sugar, then is performed with lactic acid fermentation for several ten hours to have the range of pH 4-4.5.

Description

  The present invention relates to a dry powder main raw material obtained by pulverizing and purifying organic resources generated from domestic agricultural systems and organic materials that have been partially made into industrial wastes in a hermetically sealed container. Addition of water for fermentation, hydrolyzed enzyme saccharification fluid main raw material, anaerobic and homo-type lactic acid bacteria group (Enterococcus, Lactococcus, Pediococcus, Lactobacillus), lactic acid preparation / livestock No. 1 (hereinafter, A liquid lactic acid feed for ruminant livestock for beef cattle and dairy cattle characterized by adjusting and adding sugar, etc., and lactic acid fermentation to a pH range of 4 to 4.5 It is about the method.

  Livestock feed is cereal, grain residue after harvest, rice straw, straw, bagasse, cane top, corn cobs, corn trunk, rice husk, barley straw, peanut shell, wood chips, bamboo, straw, feed rice, For several months, use green sown forage such as Italian ryegrass, legume pasture, corn for feed, grass, dry matter forage dried from these raw materials, and raw materials that have been blue chopped from early summer to early autumn in silos, roll bale wrap silos, etc. It feeds fermented lactic acid fermented long-term fermented feed, cereal-based mixed feed, concentrated feed, etc.

  Grain-based mixed feed and concentrated feed with high nutritional value are essential nutrient sources for livestock breeding. The total amount of feed to be fed per day per livestock is about 30 kg for cattle with an average weight of 600 kg, and the feed ratio of mixed feed and concentrated feed accounts for about 40%. The amount of water to be ingested is 80 kg to 100 kg in intake of about 30 kg of milk per day.

  Based on many years of research on livestock technology, feed fed to livestock is not only a source of nutrients, but also important for adding various added values. It is researched and managed from various viewpoints such as vitamins, minerals, amino acids that affect meat quality and reproduction, mold poisons and heavy metals that cause harm, as well as general nutritional components.

  In recent years, with the rise in fossil fuels, some countries that export grain as raw materials for biofuels in addition to human food and livestock feed in grain exporting countries have soared in price, making it difficult to secure livestock feed. .

  The Ministry of Agriculture, Forestry and Fisheries has increased the utilization ratio of domestic feed ingredients without relying on the increasing price of imported feed grains, and has developed technology for fermented feed with high fermentation quality (hereinafter referred to as silage) and stable feed resources. Advocates the need for securing. (See Non-Patent Document 1)

  Low-use domestic rice is also used to reduce the dependency on imported feed ingredients and improve the self-sufficiency of domestic feeds in order to prevent the infestation and prevention of various livestock infectious diseases and pests that originate from foreign sources such as foot-and-mouth disease. Encourage the expansion of the use of straw feed and the main feed.

  It is necessary to suppress the use of organic resources from domestic agricultural systems as industrial waste and to use environmentally friendly resources.

The patent literature (refer patent document 4) regarding the technique (refer patent document 1) related to the lactic-acid-fermenting bacteria for livestock grass developed for long-term preservation feed, and a vitamin additive etc. is described.
JP 2004-41064 A JP-A-6-98687 JP2009-45A (P2009-45A) JP2009-203160A (P2009-203160A)

"Joint test and information collection report on feeding technology for fattening cattle of fermented rice roughage" (Japan Society of Livestock Technology 2006, P.14-P.22, P.35-P.43) “Status of feed (use of domestic rice straw feed)” (Ministry of Agriculture, Forestry and Fisheries, 2007, Reference Material 2-P.13) “Livestock industry management information (manufactured and added technology of handmade lactic acid bacteria for promoting the promotion of feed rice)” (Corporate Society, Central Livestock Society, issued in 2004) “Grass and horticulture (Nutrition value of corn silage changed by crushing treatment. Researcher of Hokkaido Livestock Experiment Station, Tamako Tanigawa)” (Vol.55, No.2, P.15-P.18) "0-5 Development of low-cost saccharification technology for cellulosic biomass such as agricultural residue combining hydrothermal decomposition method and enzymatic decomposition method (Mitsubishi Heavy Industries, Ltd., Nippon Oil Corporation, Independent Administrative Institution) Technology Research Institute, National Agriculture and Food Research Organization, National University Corporation / Kumamoto University, pages 55-69) “Raw rice straw for feed using Tamogitake (Akira Togashi, Shozo Yoneyama, Nankai Serizawa: Lecture Collection No.22, 74-79 (1990) of the Japanese Wood Society)”

A method for producing a high-quality silage with high palatability in a short period of time without requiring a long period of time for the production of silage is desired.

Traditional long-term storage silages include grains, post-harvest cereal residues, rice straw, straw, bagasse, cane top, corn cobs, corn trunk, rice husk, barley straw, peanut shell, wood chips, bamboo, straw For example, feed rice, Italian ryegrass, corn for feed, grass, etc. are cut and lactic acid bacteria are added at the same time, and roll roll wrap silage is prepared using a silo storage or a self-propelled wrap machine. However, the green cutting material is a long tube / short tube stem / foliate, which has a large effect on the fermentation quality of silage. There is a need for improved silage.

As described above, the factors that have a significant effect on silage quality are not only the moisture contained in the raw material, but also the long and short tubular stems and leaves of the green-cutting raw material form contain a large amount of air and raw material epiphytes. Even if it sees the composition of adhering microorganisms of feed rice shown in Table 1, the number of adhering microorganisms (cfu) is 10 ⁶ to 10 ^{7 for} aerobic bacteria per 10 g of fresh material grass and 10 for Bacillus bacteria. ⁴ to 10 ⁵ , lactic acid bacteria <10 ³ bacteria level, adherent bacteria such as filamentous fungi, yeast, butyric acid bacteria, etc. are prominent, and during silage adjustment work, environmental conditions are more favorable for the growth of aerobic bacteria than anaerobic bacteria It is also necessary to improve the degradation silage, including butyric acid, ammonia nitrogen and mold.

  As shown in Table 2, soluble sugars that are nutrient sources for lactic acid bacteria in rice straw, wheat straw, corn cobs, feed rice, Italian ryegrass, and pasture, which are raw materials for silage, are farther than cereals. Therefore, it is difficult to improve the silage quality with a large amount of lactic acid unless the raw material-containing polymeric polysaccharide and the small amount of soluble saccharide are used effectively.

Dry matter raw materials, such as rice straw, wheat straw, corn cobs, feed rice, Italian ryegrass, and grass, once dried and stored as dry roughage materials are added with water for fermentation and lactic acid bacteria to be silaged later And even if you try to make lactic acid fermentation, there is almost no lactic acid fermentation. In particular, dry rice straw after harvesting rice grains has no use only for dry mastication feed for livestock or for laying barns.

In particular, roll bale wrap silage is a feed that is manufactured in a natural environment and stored outdoors, so it is difficult to maintain hermeticity even after re-wrapping after unwrapping the bale wrap and feeding it. In order to cause secondary fermentation by growth and degradation of silage, secondary addition of a lactic acid fermentation accelerating solution for quality improvement is necessary.

Grains that are mostly dependent on foreign imports are human food and are the main raw materials for blended and concentrated feeds that are indispensable for livestock breeding. At the same time, they are also used as biofuel resources. The problem of securing cereals as a feed ingredient due to soaring prices arises, and it is necessary to develop feeds with high nutritional value instead of cereals.

  Further technological development of high-quality silage using low-use domestic rice straw for the reduction and substitution of expensive grain feed is an urgently important issue.

  At present, as shown in Table 3, domestic rice straw accounts for about 90% of feed rice straw, while domestic rice straw utilization rate is about 1 million against domestic production of about 9 million tons in 2006. The remaining 90% is imported despite being swallowed and incinerated. (See Non-Patent Document 2)

  Hundreds of thousands of livestock were killed in Japan, where the infectious disease and foot-and-mouth disease that occurred in 2005 in China, the country where rice straw was imported, were devastating. In order to prevent the invasion and prevention of infectious diseases and pests, it is an urgent issue to shift from dependence on imported products with unclear safety to domestic rice straw.

Ruminant livestock feeds are silage, dry matter roughage, mixed feed, concentrated feed and most are chewing feed. Chewing feed is necessary to maintain the function of the rumen, but it is also a feed that causes dyspepsia and gastritis due to overdose. As a feed for indigestion, gastritis prevention and healing, there is also a need for a digestible and stomach-friendly feed having a form other than chewing feed.
(See Non-Patent Document 4)

  It is necessary to reduce the use of organic resources from domestic agricultural systems as industrial waste and to use environmentally friendly resources.

  The raw material for fluid lactic acid feed for ruminant livestock is pulverized by machining to facilitate enzymatic degradation and lactic acid fermentation of dry matter roughage raw materials such as grain residues after harvest, rice straw, straw, feed rice, Italian ryegrass, and grass. It is to purify a dry powder main raw material having a particle size distribution of 1.5 micrometer to 500 micrometer powder shown in Table 4 in which no fiber form remains after enzymatic degradation and lactic acid fermentation.

  The adjustment and drying of the moisture content of the raw material, which has a great influence on the conventional silage quality, cannot be adjusted on the green cutting shape of the raw material and the work process. However, if the dry powder main raw material according to the present invention is used, the appropriate water content can be accurately calculated from the weight ratio of the dry powder main raw material, and can be easily adjusted and added.

  Conventional silage production methods such as silos and roll bale wrap silos are made by adding lactic acid bacteria to long- and short-tubular stems and foliar materials at the same time as greening such as feed rice, corn for feed, Italian ryegrass, and grass. Because it is a silo storage and a roll-wrap wrapping process with lactic acid bacteria sprayed and added in a self-propelled wrapping machine, there are air traps in the long tube / short tube stem / foliage material. Anaerobic retention, another requirement for quality fermentation, was not possible on the raw material form. For this reason, various raw material-adherent bacteria such as filamentous fungi, yeast, Escherichia coli, aerobic bacteria, and butyric acid bacteria that proliferate in the production process of long-term storage silage were a major factor in the silage quality degradation. However, as a means for solving this problem, the dry powder main raw material of the present invention is a main raw material in which neither long or short tubular stems or foliar bodies nor air traps that are a living environment for aerobic bacteria exist.

The sugar content ratio of soluble saccharides, glucose, fructose, saccharose, etc., which are nutrient sources for lactic acid bacteria contained in rice straw, feed rice, and pastures, is about 0.2-0.3% in dry matter, which is 1 compared to the content of cereals. The plant fiber is decomposed into the main raw material obtained by dry-powdering the plant fiber formed from high-molecular polysaccharides cellulose, hemicellulose, and lignin, which account for about 40% of the raw material ingredients. Enzyme cellulose is added, water for hydrolysis and water for lactic acid fermentation necessary for enzymatic saccharification are added in a sealed container, hydrolyzed for several hours to several tens of hours at a temperature suitable for enzymatic saccharification, and fluidity after enzymatic saccharification An anaerobic, high-type lactic acid bacterium and sugar, which is an active source of lactic acid bacteria, are added to the main raw material as a fermentation starter in the early stage of fermentation, and fermented with stirring under temperature control suitable for lactic acid fermentation. High lactic acid content The ruminant flowable milk production feed may be easily manufactured. (See Patent Document 1) (See Non-Patent Document 5) (See Non-Patent Document 6)

Fibrinolytic enzyme cellulase (2000 fibrin disintegration force unit / 1 g) per main raw material in a closed container (PH stability shown in Table 5, PH 4-5, 30 ° C., 20% residual activity rate at 20 hours) 0 .008-0.016% and water for hydrolysis and 350-400% for lactic acid fermentation were added. Anaerobic, homo-type lactic acid bacteria (4 x 10 ¹⁰ cfu / g) fermenting sugar is the active source of 0.006% or addition and lactobacilli Star - data - as a 5% additive, even the most increase in cell count between ¹⁰ 8 cfu / g per 8 hours the temperature of 28 ° C. to 35 ° C. seen And the liquid lactic acid feed for ruminant livestock in the range of PH4-4.5 is manufactured by 48 to 100 hours fermentation, stirring.

  The dry powder main raw material of the present invention is mainly domestic raw material instead of imported opaque rice straw that is not safe, is not affected by the natural environment in the manufacturing process at all, and is harmful to the manufacturing process (radioactive pollutant, soil mass) , Removal of metal pieces, etc.), drying, sterilization and sterilization process of livestock infectious disease factors (foot-and-mouth disease virus, aerobic bacteria, Escherichia coli, filamentous fungi, various raw material adhering bacteria) due to ozone gas blowing, emphasizing the safety of feed ingredients The manufacturing process is performed.

  As a means of promoting the use of domestic rice straw, which has a low feed utilization rate instead of imported rice straw, which is less transparent, and the promotion of the use of mainland rice as a main feed and the improvement of self-sufficiency, Using dry rice straw that was not used only as dry mastication roughage, which has low palatability for livestock, as a dry powder raw material, and making it the main raw material for fluid lactic acid feed for livestock with high palatability by enzymatic saccharification and lactic acid fermentation Thus, the rice straw raw material of rice cereal residue produced in large quantities every year can be used effectively in the environment.

  The dry powder main raw material of the present invention is a plant fiber form formed from high molecular weight polysaccharides, hemicellulose, and lignin, which account for more than about 40% of the raw material components. In this raw material, plant fibrinolytic enzyme cellulose and water for hydrolysis and water for lactic acid fermentation are added to this main raw material, and the enzyme saccharification fluidity main raw material after hydrolysis is anaerobic, homo-type lactic acid bacteria, Adjusting and adding sugar and fermenting lactic acid is effective in producing a high-quality fluid lactic acid feed with a large amount of lactic acid.

  The fluid lactic acid feed for ruminant livestock of the present invention is a safe and safe dry powder purified through careful selection of raw materials, removal of contaminants and harmful substances, drying, sterilization and sterilization processes in the production process of main raw materials. Plant fibrinolytic enzyme cellulose and water for hydrolysis are added to the raw material in a sealed container and hydrolyzed under temperature control of 30 ° C. to 50 ° C. for several hours to several tens of hours. Anaerobic, homo-type lactic acid bacteria and sugar are added to the main ingredients of the sex, and then the flow for livestock in the range of PH4 to 4.5 is obtained by fermentation for 48 to 100 hours with stirring under temperature control of 28 to 35 ° C. Lactic acid feed can be produced.

  The liquid lactic acid feed for ruminant livestock of the present invention is mixed and fed into a dry matter roughage that is hardly lactic acid fermented even when added to long-term storage silage or water and lactic acid bacteria prepared in silo, roll-belt wrap silo, etc. As a novel dry matter lactic acid fermentation roughage with good palatability and digestibility, and as a high-quality lactic acid fermentation additive feed for silage, it exhibits a great effect.

  The liquid lactic acid feed for ruminant livestock of the present invention is a raw material, while the conventional dry matter roughage is a raw material form, and even after addition of a suitable amount of moisture necessary for fermentation and homo-type lactic acid bacteria excellent in fermentation, It is a feed that promotes an appetite-enhancing appetite with a fluid texture and palatability, which is different from chewing feed by enzymatic decomposition and fluid lactic acid fermentation by powdering it, and also as a weaning feed for calves It has the characteristics of a fluid lactic acid feed rich in digestibility and a large amount of lactic acid with a feeding effect.

  The liquid lactic acid feed for ruminant livestock of the present invention is similar to the property after fermentation decomposition of chewing feed in the ruminant of ruminant livestock, and as a method of feeding liquid feed, dry feed roughage, mixed feed, concentrated feed It is a highly digestible fluid lactic acid feed that is effective as a treatment feed for preventing indigestion and gastritis.

  The fluid lactic acid feed for ruminant livestock of the present invention can be recovered as a lactic acid fermentation broth by fermenting 30 to 40 times the amount of water added to the main raw material and 20% sugar in the lactic acid fermentation process of the production process. As a substitute liquid for lactic acid bacteria pre-fermented liquid (FJLB) for improving the quality of silage such as silo, roll bale wrap silo, etc.

  Hereinafter, it demonstrates in detail using the Example of this invention.

The manufacturing method of a non-fibrous body / dry powder main raw material will be described in the order of process numbers.
1. Visual inspection of the form and properties of organic resource raw materials from agricultural sources 2. Removal of harmful substances using hazardous substance removal equipment (radioactive pollutants, soil blocks, metal fragments, etc.)
3. Dry for 5 to 10 minutes in a drum-type rotary dryer with hot air temperature of 60 ° C to 80 ° C.
4. Pulverization and refinement processing to 1.5-500 micrometer powder distribution with fine pulverizer 5. Powder collection with cyclone dust collector 6. Ozone gas atmosphere sealed in sterilization / sterilization tank for 5-10 minutes, material adhesion Bacterial sterilization / sterilization treatment 7. Vacuum packaging of non-fibrous form / sterile dry powder main raw material (preserved raw material)
The above work steps 1 to 7 are a method for producing a dry powder main raw material.

The dry powder main raw material produced in Example 1 was added in a sealed container to 0.012 g of plant fibrinolytic enzyme cellulose per kg of the main raw material and 3500 cc of water for hydrolysis and water for lactic acid fermentation, and 20 at a temperature of 30 ° C. Enzymatic saccharification fluidity main raw material after time retention and hydrolysis, mixed with 0.03 g of anaerobic / homo-type lactic acid bacteria (4 × 10 ¹ ocfu / g) and 57 g of sugar as fermentation starter, and mixed at 33 ° C. Under the temperature control, fermented for 96 hours with stirring, PH4.3 fluid lactic acid feed could be produced about 4.3 kg.

  When the fluid lactic acid feed of the present invention is mixed and fed to a dry matter roughage by about 50%, the dry matter roughage becomes a chewable roughage having good digestibility and palatability for domestic animals similar to silage, and at the same time, a lactic acid beverage can be ingested instead of water. .

  Lactic acid fermentation produced by adding 3,000% to 4000% water and 20% sugar per enzyme saccharification main raw material after hydrolysis with cellulase enzyme in the production process of fluid lactic acid feed for ruminant livestock of the present invention and lactic acid fermentation The liquid is an excellent fluid lactic acid feed that can be used as a lactic acid beverage for livestock and an adjustment liquid for improving quality of deteriorated silage and a pre-fermented lactic acid bacteria (FJLB). (See Non-Patent Document 3)

,
The lactic acid fermentation fluid feed for livestock of the present invention can sufficiently contribute to the promotion of feed utilization of abundant domestic rice straw, which is a low-use resource, and is preferred for novel livestock that has a completely different form from conventional chewing roughage. It is a characteristic feed that has good digestibility and can be applied to feed for the treatment of gastritis caused by indigestion of livestock.

Claims (3)

To make it easy to hydrolyze and lactic acid ferment dry crop roughage raw materials such as cereal residue after harvesting from the agricultural system, rice straw, straw, feed rice, feed corn, Italian ryegrass, and grass Crushing and refining by mechanical processing, dry powder main raw material with a particle distribution of 1.5 micrometers to 500 micrometers fine powder, plant fiber granules of polymer polysaccharides that account for about 40% of raw material components in a sealed container Degradation enzyme cellulase and water for hydrolysis and water for lactic acid fermentation are added. Anaerobic and homo-type lactic acid bacteria group (Enterococcus, Lactococcus, Pediococcus, Lactobacillus) ) ・ (4 × 10 ¹⁰ cfu / g), lactic acid bacteria group such as lactic acid preparations and livestock No. 1, and sugar as a lactic acid fermentation starter, adjusted and added to add lactic acid A fluid lactic acid feed for ruminant livestock characterized by fermenting. Anaerobic and homozygous lactic acid bacteria with excellent fermentation in the raw material form after dry harvesting, such as post-harvest cereal residue, rice straw, wheat straw, feed rice, feed corn, Italian ryegrass, and grass In order to enable enzymatic degradation and lactic acid fermentation of dry matter roughage raw materials that are hardly fermented even when added to the appropriate amount of water necessary for lactic acid fermentation and hermetically sealed, they are pulverized and refined by mechanical processing, and the particle size distribution is 1.5 micron. Meter powder of dry powder of 500-500 micrometer granules in a sealed container, plant fiber granules consisting of cellulose, hemicellulose, and lignin, which are about 40% of the ingredients of the raw material components Fibrinolytic enzyme cellulase and water for hydrolysis Add water for lactic acid fermentation, hydrolyzed enzyme saccharification fluidity main raw material anaerobic and homo-type lactic acid bacteria (Enterococcus, Tokokkasu bacteria, Pedeiokokkasu bacteria, Lactobacillus ^{bacteria) · (4 × 10 10 cfu} / g), the group of lactic acid bacteria such as lactic acid formulation,畜草No. 1 and lactic acid fermentation Star - data - sugar adjustment mixture was added as, PH4 is lactic acid fermentation A method for producing a fluid lactic acid feed for ruminant livestock, characterized by being in the range of -4.5. Dry powder main raw material in a sealed container, plant fibrinolytic enzyme cellulose (2000 fibrin disintegration force unit / g) 0.008-0.016% per main raw material, water for hydrolysis and water for lactic acid fermentation 350-400 Enzymatic saccharification fluidity obtained by hydrolyzing plant fiber granules consisting of cellulose polysaccharide, hemicellulose, and lignin, which account for about 40% of the raw material components, at a temperature of 30 ° C. to 50 ° C. for 20 hours. Main raw materials are anaerobic and homo-type lactic acid bacteria group (Enterococcus, Lactococcus, Pediococcus, Lactobacillus), (4 × 10 ¹⁰ cfu / g), lactic acid preparations such as lactic acid preparation / livestock No. 1 0.006 % And sugar 5% as a lactic acid fermentation starter, mixed and added, and fermented for 48 to 100 hours with stirring under a temperature control of 28 to 35 ° C. to make the pH range 4 to 4.5. Features and claims 1 livestock flowable lactate feed production process rumination of claim 2 and ruminant flowable lactate feed as claimed in.