JP2012118139A5

JP2012118139A5 -

Info

Publication number: JP2012118139A5
Application number: JP2010265703A
Authority: JP
Filing date: 2010-11-29
Publication date: 2014-01-16
Anticipated expiration: 2030-11-29

Description

本発明の第１の態様は、蛍光顕微鏡用照明光学系であって、光源側から順に、光源と、照明の均一性を向上させるためのオプティカルインテグレータと、リレーレンズと、変倍レンズと、対物レンズと、を含み、変倍レンズは、可変倍率を有するレンズ、または、異なる倍率を有するレンズと切り換え可能なレンズである蛍光顕微鏡用照明光学系を提供する。
A first aspect of the present invention is an illumination optical system for a fluorescence microscope, and in order from the light source side, a light source, an optical integrator for improving illumination uniformity , a relay lens, a variable power lens, an objective lens includes a lens, a zoom lens, a lens with variable magnification, or to provide a fluorescent microscope illumination optical system is a lens and the switchable lenses having different magnifications.

本発明の第２の態様は、第１の態様に記載の蛍光顕微鏡用照明光学系において、リレーレンズは、変倍レンズがない状態で、オプティカルインテグレータの射出面と前記対物レンズの瞳面とを共役とする蛍光顕微鏡用照明光学系を提供する。
According to a second aspect of the present invention, in the illumination optical system for a fluorescence microscope according to the first aspect, the relay lens has an exit surface of the optical integrator and a pupil plane of the objective lens in a state where there is no variable power lens. Provided is an illumination optical system for a fluorescence microscope that is conjugated .

本発明の第３の態様は、第１の態様または第２の態様に記載の蛍光顕微鏡用照明光学系において、変倍レンズは、アフォーカル光学系である蛍光顕微鏡用照明光学系を提供する。
According to a third aspect of the present invention, there is provided the fluorescent microscope illumination optical system according to the first or second aspect, wherein the zoom lens is an afocal optical system .

本発明の第４の態様は、第１の態様乃至第３の態様のいずれか１つに記載の蛍光顕微鏡用照明光学系において、さらに、対物レンズの前側焦点位置と共役である視野絞りを含む蛍光顕微鏡用照明光学系を提供する。
According to a fourth aspect of the present invention, the illumination optical system for a fluorescence microscope according to any one of the first to third aspects further includes a field stop that is conjugate with the front focal position of the objective lens. Provided is an illumination optical system for a fluorescence microscope.

本発明の第５の態様は、第１の様態乃至第４の様態のいずれか１つに記載の蛍光顕微鏡用照明光学系において、さらに、光源とオプティカルインテグレータの間にコレクタレンズを含み、コレクタレンズの位置によりオプティカルインテグレータは、射出面に複数の光源の像を形成、または射出面から平行光を射出する蛍光顕微鏡用照明光学系を提供する。
本発明の第６の態様は、第１の様態乃至第４の様態のいずれか１つに記載の蛍光顕微鏡用照明光学系において、さらに、光源とオプティカルインテグレータの間に移動可能なコレクタレンズを含み、コレクタレンズの移動によりケーラー照明とクリティカル照明との間に変更可能である蛍光顕微鏡用照明光学系を提供する。
本発明の第７の態様は、第１の様態乃至第６の様態のいずれか１つに記載の蛍光顕微鏡用照明光学系において、さらに、前記リレーレンズと前記対物レンズの間に、ダイクロイックミラーを含み、前記変倍レンズは、前記リレーレンズと前記ダイクロイックミラーの間に配置される蛍光顕微鏡用照明光学系を提供する。
本発明の第８の態様は、第１の様態乃至第７の様態のいずれか１つに記載の蛍光顕微鏡用照明光学系において、前記オプティカルインテグレータは、フライアイレンズである蛍光顕微鏡用照明光学系を提供する。
本発明の第９の態様は、第１の様態乃至第８の様態のいずれか１つに記載の蛍光顕微鏡用照明光学系において、変倍レンズは、試料面への投影変倍の選択により照明範囲を任意に調整する蛍光顕微鏡用照明光学系を提供する。 According to a fifth aspect of the present invention, in the illumination optical system for a fluorescence microscope according to any one of the first to fourth aspects, the collector lens further includes a collector lens between the light source and the optical integrator. Depending on the position, the optical integrator provides an illumination optical system for a fluorescence microscope that forms images of a plurality of light sources on the exit surface or emits parallel light from the exit surface .
According to a sixth aspect of the present invention, in the illumination optical system for a fluorescence microscope according to any one of the first to fourth aspects, further includes a collector lens movable between the light source and the optical integrator. Provided is an illumination optical system for a fluorescence microscope that can be changed between Kohler illumination and critical illumination by moving a collector lens.
According to a seventh aspect of the present invention, in the illumination optical system for a fluorescence microscope according to any one of the first aspect to the sixth aspect, a dichroic mirror is further provided between the relay lens and the objective lens. And the variable magnification lens provides an illumination optical system for a fluorescence microscope disposed between the relay lens and the dichroic mirror.
An eighth aspect of the present invention is the illumination optical system for a fluorescence microscope according to any one of the first aspect to the seventh aspect, wherein the optical integrator is a fly-eye lens. I will provide a.
According to a ninth aspect of the present invention, in the illumination optical system for a fluorescence microscope according to any one of the first aspect to the eighth aspect, the variable power lens is illuminated by selecting a projection variable power on the sample surface. Provided is an illumination optical system for a fluorescence microscope that can arbitrarily adjust the range.

Claims

An illumination optical system for a fluorescence microscope, in order from the light source side,
The light source;
An optical integrator to improve lighting uniformity ,
A relay lens,
A variable power lens,
An objective lens,
Before SL zooming lens, a lens with variable magnification, or fluorescence microscope illumination optical system, which is a lens and switchable lenses having different magnifications.

The illumination optical system for a fluorescence microscope according to claim 1,
The illumination optical system for a fluorescence microscope , wherein the relay lens conjugates the exit surface of the optical integrator and the pupil surface of the objective lens in the absence of the zoom lens .

The illumination optical system for a fluorescence microscope according to claim 1 or 2,
The illumination optical system for a fluorescence microscope , wherein the zoom lens is an afocal optical system.

The illumination optical system for a fluorescence microscope according to any one of claims 1 to 3, further comprising:
An illumination optical system for a fluorescence microscope , comprising a field stop that is conjugate with a front focal position of the objective lens .

The illumination optical system for a fluorescence microscope according to any one of claims 1 to 4, further comprising:
Including a collector lens between the light source and the optical integrator;
Depending on the position of the collector lens, the optical integrator may have a plurality of front surfaces on the exit surface.
An illumination optical system for a fluorescence microscope, characterized by forming an image of a light source or emitting parallel light from an exit surface .

The illumination optical system for a fluorescence microscope according to any one of claims 1 to 4, further comprising:
A collector lens movable between the light source and the optical integrator,
It can be changed between Kohler illumination and critical illumination by moving the collector lens
An illumination optical system for a fluorescence microscope.

The illumination optical system for a fluorescence microscope according to any one of claims 1 to 6, further comprising:
A dichroic mirror is included between the relay lens and the objective lens,
The zoom lens is disposed between the relay lens and the dichroic mirror.
An illumination optical system for a fluorescence microscope.

The illumination optical system for a fluorescence microscope according to any one of claims 1 to 7,
The optical integrator is a fly-eye lens
An illumination optical system for a fluorescence microscope.

The illumination optical system for a fluorescence microscope according to any one of claims 1 to 8,
The variable magnification lens arbitrarily adjusts the illumination range by selecting the magnification of projection on the sample surface.
An illumination optical system for a fluorescence microscope.