JP2011028187A - Device for transmission fluorescence observation on stage of stereomicroscope - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a device for transmission fluorescence observation of a fluorescent sample by using a stereomicroscope of a user. <P>SOLUTION: In the device, a biological sample 6 for fluorescence observation is placed on a filter 2 for fluorescence excitation, and the biological sample 6 is irradiated with irradiation light 7 from an LED light source 1 for the fluorescence excitation by extracting only a fluorescence excitation light wavelength in the filter 2 for the fluorescence excitation. When the fluorescence light obtained by fluorescence of the biological sample is transmitted through an absorption filter 3 for fluorescence observation, the fluorescence excitation light wavelength from the filter 2 for the fluorescence excitation is absorbed and cut off by the absorption filter 3, and then only a fluorescence image reaches an objective lens 5 of the stereomicroscope. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention is a lower LED excitation light source that enables fluorescent transmission observation of, for example, a biological sample that expresses fluorescent protein or is fluorescently labeled by simply placing the apparatus between the stage of an existing stereomicroscope and an objective lens. And a unit that combines an absorption filter for upper fluorescence observation.

  Fluorescence observation with a conventional stereomicroscope incorporates an epi-illumination light source for fluorescence excitation and an absorption filter for fluorescence observation into the optical system in the stereomicroscope itself. It was necessary to purchase a new option or purchase a fluorescence option prepared by the manufacturer of the handheld microscope.

Problems to be solved by the invention

Fluorescence observation with a conventional stereomicroscope is very expensive because the stereomicroscope itself incorporates an incident light source for excitation of fluorescence and an absorption filter for fluorescence observation into the optical system.

Means for solving the problem

The present invention employs the following means in order to solve the above problems.
This unit employs a transmission illumination system that irradiates the fluorescence excitation light source from the opposite side of the biological sample as viewed from the microscope optical system, thereby observing the light source with the excitation filter in the space between the stage of the stereomicroscope and the objective lens and fluorescence observation It becomes possible to install both absorption filters.

The invention's effect

Since the structure is simple without a complicated optical system, it can be provided at low cost, and since it is simply placed on the stage of an existing stereomicroscope, there is an advantage that a user's stereomicroscope can be used as it is.

FIG. 1 is a structural sectional view of the present invention.

  For example, the biological sample 6 for fluorescence observation is placed on the filter 2 for fluorescence excitation, and the irradiation light 7 from the LED light source 1 for fluorescence excitation is extracted by the filter 2 for fluorescence excitation and only the wavelength of the fluorescence excitation light is extracted. When fluorescent light fluorescent from the biological sample passes through the absorption filter 3 for fluorescence observation, the fluorescence excitation light wavelength from the fluorescence excitation filter 2 is absorbed and blocked by the absorption filter 3, and only the fluorescent image reaches the objective lens 5 of the stereomicroscope. Fluorescence observation is possible.

DESCRIPTION OF SYMBOLS 1 LED light source for fluorescence excitation 2 Filter for fluorescence excitation 3 Absorption filter for fluorescence observation 4 Stereo microscope stage 5 Stereo microscope objective lens 6 Biological sample for fluorescence observation 7 Light irradiated from LED

Claims (1)

  An apparatus for transmission fluorescence observation, which is placed on the stage of a stereomicroscope and integrated with an LED light source, a fluorescence excitation filter, and a fluorescence absorption filter for observing a fluorescence sample with transmission fluorescence.

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