JP2011050347A - alpha-LINOLENIC ACID-ENRICHED EGG AND USE THEREOF - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide α-linolenic acid-enriched eggs capable of being confirmed, by a human intervened test, that the functions of α-linolenic acid exerting blood viscosity improving action, anti-arteriosclerotic action, obesity and constipation improving effect and antiallergic effect, and functions of wood vinegar purified solution promoting proliferation of lactobacillus present in animal intestines, are synergistically exerted, and to provide uses of the eggs. <P>SOLUTION: There are provided α-linolenic acid-enriched eggs produced by laying hen orally ingesting feed for laying hen for at least 5 weeks, which feed is provided by adding 0.5-1.5 wt.% wood vinegar purified solution and 2.0-3.0 wt.% Perilla ocymoides seed to the feed for laying hen, wherein the wood vinegar purified solution is prepared by dry-distilling wood pieces containing bark of broadleaf tree at 200-380°C, leaving the obtained crude wood vinegar solution for three months or more to separate into three layers of the light oil of the upper layer, the wood vinegar solution of the intermediate layer and the harmful tar component of the lower layer, isolating the wood vinegar solution of the intermediate layer, and separating 3-4 benzpyrene therefrom to purify. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to an α-linolenic acid-enriched egg having a high α-linolenic acid content and use thereof.

  In recent years, the percentage of people suffering from Japanese cedar pollinosis or diabetes has increased in Japan. Numerous studies and survey results have reported the relationship between these diseases and the intake of α-linolenic acid (hereinafter sometimes simply referred to as “LNA”), and the importance of LNA intake has been clarified. . On the other hand, modern Japanese people have pointed out excessive intake of n-6 fatty acids such as linoleic acid, and “Japanese dietary intake standards (2005 edition)” are n-3 fatty acids represented by LNA. In addition, a target intake amount of n-6 fatty acids is set, and it is said that in order to optimize the intake fatty acids, it is necessary to increase the intake amount and intake ratio of n-3 fatty acids in the diet.

  However, there are few foods that contain a lot of LNA, and since they are easily oxidized when ingested in the form of edible oil, there is one aspect that it is difficult to store and use at home.

  Here, chicken eggs are highly nutritious and have penetrated the Japanese diet, and it is possible to ingest LNA daily and continuously by using chicken eggs enriched with LNA as a source. .

  And regarding the effects exhibited by LNA, LNA-enriched eggs in which the content of LNA is increased, and LNA-enriched eggs, various proposals and reports have been made as follows.

  In Patent Document 1, by feeding egoma seeds, animal foods such as poultry, milk, eggs and the like having an increased LNA content can be obtained, and the content of the LNA is increased. It is described that chicken eggs and the like have an allergic reaction suppressing effect.

  Non-Patent Document 1 describes that when egoma seeds were fed to laying hens, the n-6 / n-3 ratio of fatty acid composition in egg yolk was significantly reduced.

  Non-Patent Document 2 describes that linoleic acid in the fatty acid composition of chicken meat and eggs decreases and LNA significantly increases by ingesting a feed mixed with sesame seeds.

  Non-patent document 3 shows that the level of leukotriene B4, which acts exacerbating inflammation and allergic reactions, is reduced by 25% in the low n-6 / n-3 section, reflecting the decrease in the precursor arachidonic acid. It is described.

  Non-patent document 4 reports a study on the blood glucose level lowering effect of LNA.

  Thus, it has been reported that LNA has a blood consistency improving effect, an anti-arteriosclerotic effect, an obesity and constipation improving effect, and an antiallergic effect.

  In addition, 60% of the fatty acid contained in the annual grass of Lamiaceae is LNA. By including this in the feed, the above-mentioned functionality of LNA is transferred to chicken eggs, etc., and is easily oxidized. In the past, proposals have been made to compensate for the weaknesses of LNA and to enable daily intake.

  On the other hand, when a purified vinegar solution obtained by separating and purifying harmful substances from the crude vinegar solution obtained by dry distillation of wood is administered to animals, the growth of lactic acid bacteria present in the intestines of the animals is promoted (patent document) 2) This is mixed with feed and taken orally by egg-laying hens to maintain and improve the health of egg-laying hens.

JP 63-237745 A JP 2005-318856 A

"Functional calculation tail production using egoma, non-standard rice or barley" Fukui Prefectural Livestock Experiment Station Research Report (18, 13-20 (2004)) "Effects of Sesame Seed Mixture Administration on Lipid Properties of Chicken Eggs and Chicken and Taste of Chicken" Agricultural Report of Miyagi Agricultural College (52, 29-34 (2004.12.24)) "Production of functional chicken eggs fed with waste rice, sesame seeds, etc. and effect of donation to rats" Fukui Prefectural Livestock Experiment Station Research Report (17, 12-20 (2004.03)) `` Effect of Alpha-Linolenic Acid on Blood Glucose, Insulin and GLUT4 Protein Content of Type 2 Diabetic Mice '' J Health Sci 46, 489-492 (2000.12.01)

  The present invention relates to a function of α-linolenic acid that exerts blood consistency improving action, anti-arteriosclerotic action, obesity and constipation improving effect, and anti-allergic effect, and wood vinegar that promotes the growth of lactic acid bacteria present in the intestines of animals. The object is to propose an α-linolenic acid-enriched egg that can be confirmed by a human intervention test that the function of the purified solution is synergistically exhibited and its use.

  The present invention for achieving the above object is as follows.

The invention described in claim 1
For feed for laying hens,
The crude wood vinegar obtained by dry-distilling wood chips containing broad-leaved bark at 200-380 ° C is allowed to stand for more than 3 months and separated into three layers of light oil in the upper layer, wood vinegar in the middle layer, and harmful tar in the lower layer. After the middle layer of wood vinegar was separated, 0.5 to 1.5% by weight of the wood vinegar purified solution purified by separating 3-4 benzpyrene from this,
Sesame seeds 2.0-3.0% by weight
A feed for laying hens that contains
It is an α-linolenic acid-enriched egg produced by a laying hen that has been orally ingested for at least 5 weeks.

The invention according to claim 2
The use of an α-linolenic acid-enriched egg according to claim 1, wherein the α-linolenic acid-enriched egg according to claim 1 is orally ingested at least 2 times a day for at least 6 months to exert a blood glucose level-reducing action. It is.

The invention described in claim 3
The use of the α-linolenic acid-enriched egg according to claim 1, which exhibits an allergic constitution improving effect by ingesting two or more α-linolenic acid-enriched eggs according to claim 1 per day for at least 6 months or more. .

  According to the present invention, the function of α-linolenic acid that exerts blood consistency improving action, anti-arteriosclerotic action, obesity and constipation improving effect, and anti-allergic effect, and the growth of lactic acid bacteria existing in the intestines of animals are promoted. Α-linolenic acid-enriched eggs that can be confirmed by a human intervention test that the functions of the purified vinegar to be synergistically exhibited and the use thereof can be provided

The graph which shows the result of the proliferation promotion effect test of the pyroligneous acid liquid with respect to lactic acid-producing bacteria.

(Preparation of feed for laying hens)
Sesame seeds, 0.5-1.5% by weight of a purified vinegar solution obtained by separating and purifying toxic substances from a crude vinegar solution obtained by dry distillation of wood against commercially available feed for laying hens Is mixed with 2.0 to 3.0% by weight to prepare a feed for laying hens.

  Here, egoma was produced in Shimane Prefecture (Okuzumo-cho or Kawamoto-cho).

  The purified vinegar solution was prepared as follows.

  The crude wood vinegar obtained by dry-distilling wood chips containing broad-leaved bark at 200-380 ° C is allowed to stand for more than 3 months and separated into three layers of light oil in the upper layer, wood vinegar in the middle layer, and harmful tar in the lower layer. Let Thereafter, the wood vinegar solution of the intermediate layer was separated, and 3-4 benzpyrene was separated therefrom and purified.

  From the viewpoint of the effects of the invention, the tree is preferably a piece of wood containing a bark of a broad-leaved tree. Deciduous trees such as cedar, cypress, and larch, which have not been considered useful in the past, can also be used for broadleaf trees from the viewpoint of forest protection and effective use of waste. In addition, from the viewpoint of processing heat efficiency and the like, a bark material obtained by finely pulverizing bark is preferable. Also, because it has been used as a raw material for traditional Chinese medicine, it is one of oak, tab, cherry, maple, shii, kusu, chair, oak, kihada, bayberry, oak, zelkova, mulberry, kiri, mizuki, soya It is more preferable to contain one kind.

  When a piece of wood containing hardwood bark is dry-distilled at 200 to 380 ° C., gas is generated. When the gas is collected and cooled, a crude wood vinegar can be obtained.

  If this crude wood vinegar is left for more than 3 months, it is separated into three layers of light oil in the upper layer, wood vinegar in the middle layer and harmful tar in the lower layer as described above. To separate.

  Since the separated wood vinegar liquid contains harmful substances, it is purified by distillation. Examples of the distillation method include a method of repeated distillation at 120 ° C. or lower, but are not limited thereto. 3-4 Benzpyrene, which is a harmful substance, was removed from the wood vinegar solution to obtain a wood vinegar purified solution.

An example of the properties of the purified vinegar solution is shown in Table 1.

  In the above, 0.5 to 1.5% by weight of purified vinegar to be added to and mixed with commercially available feed for laying hens, and 2.0 to 3.0% by weight of sesame seeds, The function of the above-described wood vinegar refinement solution that allows the lactic acid bacteria present in the intestine of the laying hen to be ingested orally to maintain and enhance the health of the laying hen, and transferred to the hen's egg. This is from the viewpoint of synergistically exhibiting the blood consistency improving action, anti-arteriosclerotic action, obesity and constipation improving effect and anti-allergic effect of α-linolenic acid. When the blending ratio is less than these, the intended function / effect is not sufficiently exhibited. On the other hand, the blending ratio is recognized to be sufficient from the viewpoint of cost and effect.

(Feeding and collecting eggs)
The fatty acid analysis of the egg produced by the laying hen was conducted while feeding the purified vinegar and the feed for the sesame seed-containing laying hen as described above to the laying hen.

  After 5 weeks from the start of feeding, the LNA content in the yolk reached a stable level, and the LNA content was 170-240 mg per piece (hereinafter, the feed for egg laying hens containing the purified vinegar and sesame seeds was fed for 5 weeks. Eggs produced by laying hens (LNA content: 170-240 mg / piece) are referred to as “LNA-enriched eggs”).

  As a comparison object, 0.5 to 1.5% by weight of purified vinegar was added and mixed in the same manner as described above, but no sesame seeds were added or mixed. The fatty acid analysis was also performed on the eggs to be processed, and the LNA content in the yolk was about 30 mg / piece even after 5 weeks from the start of feeding (hereinafter, 0.5 to 1.5% by weight of a purified vinegar solution was added) -Eggs produced by laying hens that have been fed commercially available feed for laying hens with mixed but not added sesame seeds are referred to as "normal eggs").

(Method of oral intake by humans, taste test)
As for LNA-enriched eggs, in the case of raw food, when the LNA content of boiled eggs cooked using this and hot spring eggs were compared, the residual ratio was high, and it was considered that these eating methods were desirable.

  In addition, when normal eggs and LNA-enriched eggs were boiled eggs, and the taste was compared, no difference in taste was found between the normal eggs and LNA-enriched eggs.

(Human intervention study)
Intervention trial participants were extracted from the results of prior lifestyle surveys and blood tests, and allergen positive participants found by the initial screening were taken into the group that ingested normal eggs (normal egg intake group: 51 people: 33 men) 18 women) and LNA-enriched eggs (LNA-enriched egg intake group: 51 people: 33 men / 18 women). Each participant received 6 normal eggs and LNA-enriched eggs. The human intervention study was conducted on the following schedule over 6 months.

(Schedule)
First medical examination briefing session. Blood collection. Screening of intervention trial participants About 2 months after initial screening
Briefing session for intervention participants
Intervention test started 3 months after the start of the intervention test Screening and blood sampling 6 months after the start of the intervention test Screening and blood sampling (survey items)
Screening: Interview, height, weight, body fat percentage, blood pressure, waist circumference, visual acuity Blood analysis:
Biochemical test Total cholesterol, LDL-cholestrol, HDL-cholestrol, triglyceride, blood glucose, glycohemoglobin A1c, urea nitrogen, creatinine, albumin, GOT, GPT, γGTP
Allergen test Cedar pollen, mushroom mite, house dust, egg white, shrimp Fatty acid analysis Fatty acid content and composition of plasma and erythrocyte membrane Serum bone metabolism marker Egg consumption: number and how to eat (results of human intervention study)
At 3 months and 6 months after the intake, the LNA-enriched egg intake group increased the erythrocyte membrane DHA content compared to the normal egg intake group.

  It was suggested that blood glucose was significantly reduced in the LNA-enriched egg intake group 6 months after the intake compared to the normal egg intake group.

  Six months after the ingestion, the LNA-enriched egg ingestion group had fewer allergen antibody titers than the normal egg ingestion group, suggesting that the LNA-enriched egg improved the allergic constitution.

(Preparation of purified vinegar)
25 tons of dry wood pieces containing fresh oak, shii, tub, cherry and maple bark were used as raw materials and placed in a dry distillation furnace and subjected to dry distillation at 350 ° C. for 168 hours.

  The gas generated during dry distillation was collected, and a crude wood vinegar solution was obtained by natural cooling.

  The obtained crude wood vinegar was allowed to stand in a separation tank for 3 months or longer to obtain a separated crude wood vinegar separated into three layers of an upper layer of light oil, an intermediate layer of wood vinegar, and a lower layer of harmful tar.

  An intermediate layer of wood vinegar was separated from the separated crude wood vinegar to obtain a wood vinegar.

  The vinegar solution was purified by repeated distillation at 120 ° C. or lower until no harmful substances were detected, and about 1 ton of vinegar solution was obtained.

  This purified vinegar solution was a colorless and transparent liquid, had a unique burning odor and acidity, and its pH at 18 ° C. measured by a digital pH meter was 3.7. In addition, when 1 ml of sulfuric acid was added to 5 ml of purified vinegar and heated, the smell of acetic acid was emitted. Furthermore, when sodium hydroxide test solution was added to 5 ml of purified wood vinegar for neutralization and ferric chloride test solution was added dropwise, the solution became reddish brown and boiled, resulting in a reddish brown precipitate.

In order to clarify the effect of purified vinegar on normal bacterial flora (lactic acid producing bacteria), was prepared 13 pieces of medium 10 ⁷ inoculation faecium, we divided them into nine and four two groups. In each of the nine culture media, purified vinegar: 0%, 0.25%, 0.5%, 1%, 2%, acetic acid: 0.25%, 0.5%, 1 wt% and 2 wt% were added, and then the pH of each was set to 7 to examine the growth of lactic acid producing bacteria. In addition, acetic acid: 0.25 wt%, 0.5 wt%, 1 wt%, 2 wt% was added to each of the four media, and the growth of lactic acid-producing bacteria was examined without adjusting the pH. The result is shown in FIG.

  As the amount of the purified vinegar solution increased, the growth of lactic acid-producing bacteria was promoted, but acetic acid had no effect on the growth of lactic acid-producing bacteria, but rather showed a growth inhibitory effect.

  From this, it was confirmed that the pyroligneous acid purified solution showed a growth promoting effect against lactic acid-producing bacteria.

(Preparation of feed for laying hens)
To 100 kg of laying hen feed commercially available, 0.1 kg of the purified vinegar solution prepared as described above and 2.5 kg of sesame seeds from Kawamoto-cho, Shimane are added and mixed to prepare feed for laying hen did.

(Feeding and collecting eggs)
Compared with LNA-enriched eggs produced by laying hens that had been supplied with the purified vinegar and sesame seed-containing laying hen feed as described above for 5 weeks, and that had been ingested for 5 weeks. Therefore, in the same manner as described above, 100 kg of commercially available feed for laying hens was added and mixed with 0.1 kg of pyroligneous acid but no sesame seeds were added or mixed for 5 weeks. The normal eggs produced by the laying hens that had been ingested over a long period of time were subjected to the human intervention test, and the human intervention test was conducted according to the schedule and survey items.

The results are shown in Table 2 below.

  As a result of the human intervention test, it was confirmed that the highest level group in the cedar pollen positive group showed significantly lower values of LNA, EPA, and DHA in plasma than the negative group.

  In addition, the erythrocyte membrane DHA content increases in the normal egg intake group and the LNA-enriched egg intake group 6 months after the intake, but the increase in the LNA-enriched egg intake group is significantly higher than that in the normal egg intake group. Indicated.

  Furthermore, after 6 months of intake, blood glucose levels increased in both the normal egg intake group and the LNA-enriched egg intake group, but the increase was significantly lower in the LNA-enriched egg intake group than in the normal egg intake group. It was done. From this, it was suggested that the effect of reducing blood glucose level may be exerted by ingesting two or more LNA acid-enriched eggs per day for at least 6 months or more.

  In addition, after 6 months of intake, both the normal egg intake group and the LNA-enriched egg intake group had decreased allergen-positive class levels, but the percentage of humans that did not decrease was that the normal egg intake group was the LNA-enriched egg intake group. There were significantly more than From this, it was suggested that the allergy constitution improving effect may be exhibited by orally ingesting 2 or more LNA-enriched eggs per day for at least 6 months or more.

  The preferred embodiments and examples of the present invention have been described above. However, the present invention is not limited to the embodiments and examples, and various forms are possible within the technical scope grasped from the description of the claims. Can be changed.

Claims (3)

For feed for laying hens,
The crude wood vinegar obtained by dry-distilling wood chips containing broad-leaved bark at 200-380 ° C is allowed to stand for more than 3 months and separated into three layers of light oil in the upper layer, wood vinegar in the middle layer, and harmful tar in the lower layer. After the middle layer of wood vinegar was separated, 0.5 to 1.5% by weight of the wood vinegar purified solution purified by separating 3-4 benzpyrene from this,
Sesame seeds 2.0-3.0% by weight
A feed for laying hens that contains
Α-linolenic acid-enriched eggs produced by laying hens taken orally for at least 5 weeks or more.   The use of an α-linolenic acid-enriched egg according to claim 1, wherein the α-linolenic acid-enriched egg according to claim 1 is orally ingested at least 2 times a day for at least 6 months to exert a blood glucose level-reducing action. .   The use of the α-linolenic acid-enriched egg according to claim 1, wherein the effect of improving the allergic constitution is exhibited by orally ingesting two or more α-linolenic acid-enriched eggs according to claim 1 per day for at least 6 months or more.

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