JP2010248149A - Anti-fatigue agent or physical fitness improver containing Morohaya extract - Google Patents

Abstract

To provide a new anti-fatigue agent or physical strength improver.
An anti-fatigue agent or a physical fitness improver containing an enzyme-treated moroheiya extract, and a pharmaceutical or food containing the anti-fatigue agent or physical fitness improver. The enzyme-treated morohea extract may be a morohea enzyme-treated extract solution after the morohea extract solution is enzyme-treated, or may be a solution obtained by diluting the morohea enzyme-treated extract solution. It may be a solid material obtained by making it. Moreover, protease is mentioned as an enzyme.
[Selection figure] None

Description

  The present invention relates to an anti-fatigue agent or a physical strength improver containing a moroheiya extract. Specifically, this invention relates to the anti-fatigue agent or physical strength improving agent for pharmaceuticals and foodstuffs containing a moroheiya extract.

In recent years, foods have been used for treatment or prevention methods that can be said to be intermediate between drug therapy and general therapy. Such foods are called health foods, functional foods, health supplements, and foods for specified health use.
Among these foods, there are foods that are taken daily and added with substances having a blood pressure lowering action and a fat absorption inhibiting action, and are sold as health foods, beverages and the like.
There is a growing need for foods that have therapeutic or preventive effects, and there is always a need for products that are more effective, products that can be taken safely and for a long time, and products with less economic burden.

  Peptides as disclosed in Patent Documents 1 and 2 are known as food components having an effect on recovery from fatigue and the like, as naturally derived peptides such as astaxanthin, egg white, soybean protein, and casein, and peptides derived from meat proteins. . In addition, beverages such as nutritional drinks containing herbal medicines and herbal medicines are also sold for the purpose of nutrition and tonic.

Morohaya is a kind of jute that originates in the Mediterranean region, centering on Egypt, and is one of the food materials that has attracted particular attention in recent years due to its high nutritional value. When Morohaya is finely chopped or boiled, it produces a unique slime like okra and yam, and this slime contains plant gum and mucous polysaccharide (mucopolysaccharide). Nutritionally, it is rich in vitamins and minerals, and especially has a high total carotene and calcium content. It is said that carotene is about 12 times broccoli, vitamin B ₁ is about 3 times tomato, vitamin B ₂ is about 14 times pepper, calcium is about 2.4 times milk and dietary fiber is about 20 times lettuce. Yes. Therefore, recently, it has been cultivated in Japan, and its dry powder is gaining attention as a food material along with fresh leaves.
To date, there have been proposed a large number of Morohaya extracts, Morohaya powders obtained by drying them, so-called Morohaya extracts, and foods and compositions containing them.

For example, Patent Literature 3 discloses a lipid metabolism-improving composition containing, as an active ingredient, an ethanol precipitate of a water-soluble fraction from Morohaya leaves or a dry powder thereof.
Patent Document 4 discloses a composition comprising, as an essential component, an aqueous extract of Morohaya having a gastric mucosa protective action.
Patent Document 5 discloses a fatty acid extract of Morohaya used as an NO production inhibitory activator.
Furthermore, Patent Document 6 discloses a therapeutic or prophylactic agent for a disease accompanied by modulation of insulin amount or insulin response, which contains a processed product derived from Morohaya as an active ingredient.

Japanese Patent No. 3563899 JP 2007-228875 A JP 7-69910 A JP 2000-26305 A JP 11-322667 A International Publication No. 2004/100969 Pamphlet

However, from the viewpoint of providing products that are effective, products that can be taken for a long time with peace of mind, and products that have less economic burden, there are more excellent foods that can replace the previously known peptides and the like. It is desired. Moreover, since the nutrition drink as mentioned above mix | blends a crude drug, since it is a medicinal smell and it is hard to drink, development of a component with less smell is desired.
Furthermore, although Morohaya is known as a food material with high nutritional value, it is not known about having a fatigue recovery effect or the like.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that a moroheiya extract has an anti-fatigue effect and a physical strength improving effect, and completed the present invention.

The present invention is as follows.
[1]
An anti-fatigue agent or a physical strength improver containing a moroheiya extract obtained by enzyme treatment.
[2]
The anti-fatigue agent or physical fitness improver according to [1], wherein the Morohaya extract contains a Morohaya synthetic resin adsorption fraction.
[3]
The anti-fatigue agent or physical fitness improver according to [1] or [2], wherein the enzyme is a protease.
[4]
The composition for anti-fatigue or a physical strength improvement containing the anti-fatigue agent or physical strength improvement agent of any one of [1]-[3].
[5]
Food / beverage products for anti-fatigue or physical strength improvement containing the anti-fatigue agent or physical fitness improver of any one of [1]-[3].

  According to the present invention, a new anti-fatigue agent and physical strength improver can be provided.

The measurement result of the limit travel time before ingestion of Moroheiya extract and after ingestion is shown. The result of the extension time of the limit travel time before and after ingestion of Morohaya extract is shown. The measurement result of the limit travel time before ingestion of Moroheiya extract and after ingestion is shown. The result of the extension time of the limit travel time before and after ingestion of Morohaya extract is shown.

  Hereinafter, embodiments for carrying out the present invention will be described in detail. In addition, this invention is not limited to the following embodiment, It can implement by changing variously within the range of the summary.

The anti-fatigue agent or physical fitness improver of the present invention contains a morohea extract obtained by enzyme treatment (hereinafter, the morohea extract obtained by enzyme treatment is simply abbreviated as “morohaya extract”). There is.)
In the present invention, Moroheiya is an annual herb of the family Lindenaceae, and its scientific name is Corchorus olitorius L. It is.
Morohaya is a kind of jute that originates in the Mediterranean region centering on Egypt.

The morohea extract obtained by the enzyme treatment in the present invention is obtained by enzyme treatment of the morohea extract solution.
The morohea extract may be a morohea enzyme-treated extract solution after the morohea extract solution is subjected to enzyme treatment, or may be a solution obtained by diluting the morohea enzyme-treated extract solution. It may be a solid.
Examples of the enzyme include protease.
Proteases are proteolytic enzymes that catalyze the hydrolysis reaction of peptide bonds. Although it does not specifically limit as protease, For example, the complex enzyme which mainly has an endopeptidase or an endopeptidase is mentioned.
Among them, a protease produced by Bacillus genus is preferable, and a protease produced by Bacillus subtilis or Bacillus thermoproteolyticus is more preferable. As the protease, one kind of protease may be used, or a plurality of proteases may be used in combination.

The method for enzyme-treating the moroheiya extract solution can be performed according to a conventionally known method, and is not particularly limited.
Examples of the enzyme treatment method include the following methods.
The morohea extract solution is heat sterilized at a temperature of 95 ° C. or higher and then cooled to an appropriate temperature. While maintaining the liquid temperature in the water bath, an appropriate amount of protease dissolved in a small amount of water is added.
From the addition of the protease, for example, the enzyme treatment is performed with stirring every 10 minutes. Thereafter, the enzyme is deactivated by heating, solid-liquid separation is performed by an arbitrary method such as filtration, and the extract is recovered to obtain a moroheia enzyme-treated extract solution as a moroheiya extract.

The treatment conditions such as the treatment temperature, treatment time, enzyme concentration, and pH during the protease treatment may be adjusted so that the protease to be used works optimally, and is not particularly limited.
The treatment temperature during the protease treatment is preferably 40 ° C to 70 ° C, more preferably 50 ° C to 65 ° C.
The treatment time for the protease treatment is preferably 15 minutes to 2 hours, more preferably 45 minutes to 90 minutes at a suitable temperature.
The enzyme concentration during the protease treatment is preferably 0.01 to 3% by weight, more preferably 0.05 to 2% by weight, still more preferably 0.1 to 1.5% by weight, based on the dry weight of moroheiya. It is.
The pH during the protease treatment is preferably pH 5.0 to 8.5, more preferably pH 5.5 to 7.0.

For example, when a protease produced by Bacillus subtilis is used, the treatment temperature is 55 ° C. to 60 ° C., the treatment time is 1 hour to 2 hours, and the treatment concentration is 0.1 to 1.0% by weight relative to the dry weight of moroheiya. It is preferable to perform enzyme treatment at pH 6.0 to 7.0.
When a protease produced by Bacillus thermoproteolyticus is used, the treatment temperature is 60 ° C. to 70 ° C., the treatment time is 1 hour to 2 hours, the treatment concentration is 0.1 to 1.0% by weight relative to the dry weight of moroheiya, pH 6 It is preferable to perform enzyme treatment at 0.0 to 7.0.

The method for inactivating the enzyme is not particularly limited, and examples thereof include a method for inactivating the protease by maintaining at 95 ° C. to boiling for 5 to 10 minutes.
The method of solid-liquid separation is not particularly limited, and examples thereof include a method of suction filtration using a filter paper after filtration through a sieve having an opening of 100 μm to 1 mm. And a method of suction filtration using the filter paper of No. 2.

The moroheiya extract solution is not particularly limited as long as it is a solution containing moroheiya extract components.
The moroheiya extract component is extracted from plants such as leaves, stems, flowers, and skins of the above-ground part excluding moroheiya seeds. The Morohaya extract component is preferably a Morohaya leaf extract component using a leaf portion from which a hard stem portion has been removed.
The moroheiya extract component may be an extract component extracted directly from a raw plant or an extract component extracted from a plant once dried. Moreover, a pure material can also be used as a moroheiya extract component.

The method for obtaining the morohea extract solution is not particularly limited, and the morohea extract solution can be obtained by a known extraction method.
Examples of the moroheiya extract solution include moroheiya extract obtained by extracting water or hot water from moroheiya, a morohea powder solution obtained by dissolving or mixing moroheiya powder in water or hot water, and moroheiya puree solution.

  As a morohaya extract solution, solid content, such as morohaya leaf, may be included, and the solution which removed solid content, such as morohaya leaf, may be sufficient. As the morohea extract solution, it is preferable to use a solution obtained by solid-liquid separation after filtration to remove morohea leaves and the like.

The moroheiya extract can be obtained, for example, by extracting moroheiya leaves by adding them to water or warm water and immersing them.
More specifically, after adding water or warm water to the morohaya leaves from which the hard stem portion has been removed, steam heating, crushing the morohaya leaves, and then drying the crushed morohaya leaves with hot air It can be obtained by adding 10 to 40 times by weight, preferably 20 to 30 times by weight, hot water to dry leaves and extracting them.
Moreover, a moroheiya extract can also be obtained by adding water or warm water to moroheiya puree and extracting it.

  The morohea powder may be a commercially available morohea extract powder or powdered morohea dried leaves.

In this invention, it is preferable that the morohaya extract obtained by carrying out an enzyme treatment contains the morohaya synthetic resin adsorption fraction obtained by performing a synthetic resin adsorption process further.
The moroheiya synthetic resin adsorbed fraction composition containing the moroheia synthetic resin adsorbed fraction is obtained by enzymatic treatment of the moroheiya extract solution, followed by adsorption treatment with a synthetic resin adsorbent, and elution treatment of the adsorbed fraction with an elution solvent. be able to.
The moroheiya synthetic resin adsorbed fraction composition of the present invention may be obtained by subjecting the moroheiya extract solution to an adsorption treatment with a synthetic resin adsorbent and then an enzyme treatment.

The method for performing the synthetic resin adsorption treatment can be performed according to a conventionally known method, and is not particularly limited.
Examples of the synthetic resin adsorption treatment include the following methods.
The morohea enzyme-treated extract solution is adsorbed with a synthetic resin adsorbent, and water is passed through the synthetic resin adsorbent, followed by water pushing. By recovering, a target morohea synthetic resin adsorption fraction composition can be obtained.
In the present invention, when the synthetic resin adsorption treatment is performed prior to the enzyme treatment, first, the synthetic resin adsorption treatment is performed on the morohea extract extract in the same manner as in the above method. Next, a moroheiya synthetic resin adsorbed fraction composition can be obtained by performing an enzyme treatment in the same manner as for the moroheiya extract extract.

The treatment with the synthetic resin adsorbent may be performed by either a batch method or a column method, but a column method capable of efficiently treating with a relatively small amount of the synthetic resin adsorbent is preferable.
The treatment with the synthetic resin adsorbent may be performed at least once.

The synthetic resin adsorbent is preferably a porous adsorbent suitable for adsorbing water-soluble low-molecular substances.
The specific surface area of the synthetic resin adsorbent is preferably 100m ² / g~1200m ² / g, more preferably 250m ² / g~900m ² / g approximately.
The pore volume, particle size distribution, and most frequent radius of the synthetic resin adsorbent are 0.9 mL / g to 1.6 mL / g, respectively, and 250 μm or more is preferably 90% or more and 30 to 260 μm.

Examples of the synthetic resin adsorbent include a hydrophilic synthetic resin adsorbent and a hydrophobic synthetic resin adsorbent, and a hydrophobic synthetic resin adsorbent is preferable.
Examples of the resin matrix of the hydrophilic synthetic resin adsorbent include styrene-based macroporous, silica, and methacrylic acid ester polymers.
Specific examples of the hydrophilic synthetic resin adsorbent include Diaion (registered trademark; Mitsubishi Chemical Corporation), Muromac (registered trademark; Muromachi Technos Co., Ltd.) and the like.
Examples of the resin matrix of the hydrophobic synthetic resin adsorbent include styrene polymer, styrene-divinylbenzene copolymer, styrene-acrylic acid amide copolymer, phenol resin, and the like.
As the hydrophobic synthetic resin adsorbent, for example, a porous modified polystyrene synthetic adsorbent obtained by chemically modifying and bonding a polar group such as bromine to an aromatic resin matrix such as styrene is suitable.
Specific examples of the hydrophobic synthetic resin adsorbent include Sepabeads (registered trademark; Mitsubishi Chemical Corporation), among which Sepabeads SP70, SP700, SP850, SP207, and more preferably SP207 (specific surface area 590 m). ² / g, pore volume 1.1 mL / g, particle size distribution 250 μm or more is 90% or more, and the most frequent radius is 120 mm.

The synthetic resin adsorbent may be pretreated prior to the adsorption treatment.
The pretreatment can be performed, for example, by washing the synthetic resin adsorbent with a solvent such as methanol or ethanol to remove impurities, and further washing with water to remove the solvent such as methanol or ethanol.
For washing the synthetic resin adsorbent, ethanol is preferably used.

  The ratio between the synthetic resin adsorbent and the extract such as the moroheia enzyme-treated extract solution can be selected according to the type of adsorbent used.

As the solvent used for the elution treatment, an elution solvent suitable for the synthetic resin adsorbent to be used can be appropriately selected.
For example, when an aromatic synthetic resin adsorbent is used, a large amount of water or warm water may be used as an elution solvent. In order to increase elution efficiency, the concentration is preferably 50 to 70%, more preferably Ethanol diluted with water to a concentration of 55 to 65% may be used.

More specifically, after passing the Morohaya extract solution or Morohaya enzyme-treated extract solution through a synthetic resin adsorbent-filled column, the column discharge liquid is Brix 1% or less, preferably Brix 0.5% or less as measured by Brix. The adsorbed fraction may be recovered by washing with pure water and eluting with an elution buffer such as ethanol at an appropriate concentration.
For example, in the case of a 60% ethanol solution, the adsorption fraction is collected by passing 1 to 10 times the resin volume, more preferably 1 to 5 times, and even more preferably 1 to 2.5 times. Adsorbed material can be collected efficiently. Further, when water is used, the adsorbate can be recovered by washing the morohea extract remaining in the resin with pure water and then passing it through at least three times the resin volume.

In the synthetic resin adsorption treatment, a filtration operation may be performed as necessary. For example, after filtration through a sieve having an opening of 850 μm, for example, suction filtration can be performed using No. 2 filter paper. .
The moroheia synthetic resin adsorbed fraction composition obtained by the synthetic resin adsorption treatment may be further purified. For example, the composition may be further purified by cation exchange column chromatography or gel filtration chromatography.
The synthetic resin adsorbed fraction recovered by the synthetic resin adsorption treatment may be concentrated under reduced pressure to an appropriate concentration, or may be freeze-dried into a powder.

In the present invention, when the morohea extract obtained by the enzyme treatment is obtained as a solution, it can be used as it is as a beverage or in other fruit juices, vegetable juices and mixed juices.
The moroheiya extract can be made into a concentrated liquid composition by concentrating under reduced pressure to an appropriate concentration.
The moroheiya extract obtained by the enzyme treatment may be obtained by drying a solution-like product, and is excellent in storage stability by making a dry product. The dried product can be used as it is as a food or drink, or in other fruit juices, vegetable juices and mixed juices.

  The moroheiya extract of this invention can be used as a pharmaceutical or food-drinks as an anti-fatigue composition or a physical strength improvement composition.

The morohea extract of the present invention can be used as food and drink, as food for specified insurance, nutritional functional food, health food, functional food, health supplement, etc. Can do.
The food / beverage product may be a food / beverage product with an indication that it has an anti-fatigue or physical strength improving effect.
Preferred forms as food and drink are foods and beverages such as candy, jelly, tablet confectionery, beverages, soup, noodles, rice crackers, Japanese confectionery, frozen confectionery, baked confectionery, etc., preferably fruit juice beverages, vegetable juices, fruit vegetable juices, teas It is a packaged beverage such as a beverage, a coffee beverage or a sports drink.

  Morohaya extract can be ingested by oral administration in liquid or solid form due to its low taste specific to taste and smell. , Granules, tablets, capsules and the like.

  When the morohea extract is contained in the beverage, it is preferably 50 mg to 2500 mg, more preferably 100 mg to 800 mg, in terms of dry matter per 500 mL. Moreover, it can be set as the formulation suitably mixed with the other additive as a foodstuff or a pharmaceutical so that the following intake or dosage can be achieved.

The intake of Morohaya extract can be appropriately adjusted according to the use, but in terms of dry matter, it is preferably 10 mg to 2000 mg at a time, more preferably 50 mg to 1000 mg at a time, more preferably 100 mg at a time. ~ 500 mg.
The number of intakes is not particularly limited, but is preferably 1 to 3 times a day, and the number of intakes may be increased or decreased as necessary.

The moroheiya extract of the present invention can be used as a pharmaceutical preparation by adding a pharmaceutically acceptable excipient as a pharmaceutical.
As a pharmaceutical preparation, it can be formulated into a known dosage form such as powder, granule or tablet, and can also be used as a liquid preparation such as liquid or paste.

  Pharmaceutical preparations include tablets, capsules, granules, fine granules, powders, solutions, syrups, chews, lozenges and other oral preparations, ointments, gels, creams, patches, external preparations, injections , Sublinguals, inhalants, eye drops, suppositories and the like. The dosage form of the pharmaceutical preparation is preferably a tablet, capsule, powder, granule, or injection.

The pharmaceutical preparation is useful for an anti-fatigue action and a physical strength improving action in animals, especially mammals, and can be administered to animals, especially mammals.
Examples of mammals include humans, dogs, cats, cows, horses and the like, with humans being preferred.
The dose of Morohaya extract can be appropriately adjusted according to various conditions such as the activity of individual drugs, patient symptoms, age, body weight, etc., for example, in terms of dry matter, preferably 10 mg to 2000 mg at a time. Yes, more preferably 50 mg to 1000 mg at a time, and even more preferably 100 mg to 500 mg at a time.
The number of administrations is not particularly limited, but is preferably 1 to 3 times a day, and the number of administrations may be increased or decreased as necessary.

Since the medicinal products and foods and drinks containing the moroheiya extract of the present invention have an anti-fatigue effect and a physical strength improving effect, they can be used as compositions for fatigue recovery, physical strength enhancement, nutrition tonicity, and endurance improvement.
In the present invention, the anti-fatigue action and the physical strength improving action can be confirmed by extending the limit travel time by a limit travel time confirmation test.
It is a matter known to those skilled in the art that when the limit travel time is extended by a limit travel time test using a mouse, it can be evaluated that it has an anti-fatigue action and a physical strength improvement action.

In the present invention, “fatigue” refers to a phenomenon in which the function of a living body is reduced as a result of exerting a function.
Examples of fatigue include "physical fatigue after swimming", "mental fatigue after long hours of intellectual labor", and "physical and mental combined fatigue that accumulates in everyday life". Can be mentioned.
“Anti-fatigue action” refers to an action for reducing the fatigue state and an action for promoting recovery from the fatigue state, or an action for preventing fatigue and making it less fatigued.
In the present invention, “physical strength” refers to the ability to continuously perform a certain exercise state.
“Physical strength improving action” refers to an action or the like that improves the ability to continue exercise.
In the present invention, when Morohaya extract has anti-fatigue action and physical strength improvement action, when administered or ingested to animals, anti-fatigue action, physical strength improvement action, fatigue recovery action, physical strength enhancement action, endurance improvement action , Can exert nourishing tonic effect.

  EXAMPLES Hereinafter, although an Example and a comparative example demonstrate this invention concretely, this invention is not limited to these Examples at all.

[Production Example 1]
(Morohaya extract: without enzyme treatment)
A synthetic adsorbent SP207 (manufactured by Mitsubishi Chemical Corporation) in a wet state in pure water was packed into a glass column, and sufficient pure water was passed through to form an SP207-filled column.
Morohaya leaves from which hard stems had been removed were steam-heated and then crushed and dried with hot air to obtain dried Morohaya leaves. Water of about 60 ° C distilled water of about 20 times weight is added to 50 g of the dried moroheiya leaves, heated to 95 ° C or higher, sterilized, cooled to 60 ° C, and then heated to 60 ° C in a water bath. Stirring was carried out every 10 minutes while maintaining. After 1 hour, the temperature was maintained at 95 ° C. for 5 minutes, filtered through a sieve having an opening of 850 μm, Using the filter paper of No. 2, suction filtration was performed to obtain a Morohella extract solution.
After 300 ml of the obtained morohea extract solution (Brix 0.9, pH 6.0) was passed through the glass column at a rate of SV = 4, the morohea extract solution was sufficiently washed out with 10 bed volumes of pure water. The column effluent was collected immediately after the beginning of the passage of the moroheiya extract solution, and used as a flow-through fraction. Next, 5 bed volumes of ethanol adjusted to 60% concentration were passed through, and after ½ bed volume of column effluent was discarded from the start of passage, collection of column effluent was started and collected until the end of ethanol passage. This was taken as an adsorption fraction. The flow-through fraction and the adsorbed fraction were each concentrated under reduced pressure and then lyophilized to obtain 1.9 g of a powdery flow-through extract (Ae1) and 0.29 g of an adsorbed fraction extract (Ae2). Note that “bed” indicates the filling volume (= bulk) of the adsorbent.

[Production Example 2]
(Morohaya extract: with enzyme treatment)
A synthetic adsorbent SP207 (manufactured by Mitsubishi Chemical Corporation) in a wet state in pure water was packed into a glass column, and sufficient pure water was passed through to form an SP207-filled column.
Morohaya leaves from which hard stems had been removed were steam-heated and then crushed and dried with hot air to obtain dried Morohaya leaves. Water of about 60 ° C distilled water of about 20 times weight is added to 50 g of the dried moroheiya leaves, heated to 95 ° C or higher, sterilized, cooled to 60 ° C, and then heated to 60 ° C in a water bath. Then, 0.7% by weight of protease N Amano G (manufactured by Amano Enzyme Co., Ltd.) was dissolved in a small amount of water and added to the raw material Morohaya dried leaves. Thereafter, stirring was performed every 10 minutes while maintaining the liquid temperature at 60 ° C. One hour after the addition of the enzyme, the mixture was maintained at 96 ° C. for 5 minutes, filtered through a sieve having an opening of 850 μm, Using the filter paper of No. 2, suction filtration was performed to obtain a Morohaya enzyme-treated extract solution.
After 300 ml of the resulting moroheia enzyme-treated extract solution (Brix1.2, pH 6.0) was passed through the glass column at a rate of SV = 4, the moroheia extract was sufficiently washed out with 10 bed volumes of pure water. The column effluent was collected immediately after the beginning of the passage of the morohaya enzyme-treated extract solution, and used as a passing fraction. Next, 5 bed volumes of ethanol adjusted to 60% concentration were passed through, and after ½ bed volume of column effluent was discarded from the start of passage, collection of column effluent was started and collected until the end of ethanol passage. This was taken as an adsorption fraction. The flow-through fraction and the adsorbed fraction were each concentrated under reduced pressure and then lyophilized to obtain 2.1 g of a powdery flow-through fraction extract (Be1) and 0.8 g of an adsorbed fraction extract (Be2). Note that “bed” indicates the filling volume (= bulk) of the adsorbent.

For the measurement of the limit running time with the following treadmill, mice (CDF1, male, 6 weeks old; Nippon SLC Co., Ltd.) were used after preliminary breeding for 1 week.
During the experiment, mice were allowed to freely take water and feed (CE-2; Nippon Claire Co., Ltd.).

[Example 1]
(Measurement of limit travel time by treadmill)
The following experiment was conducted with the group to which Be2 obtained by the method of Production Example 2 was administered as the Morohaya extract MP group and the group to which distilled water was administered as the control group.
The mice were forcibly run for 30 minutes a day and trained for 5 days according to the running speed time program shown in Table 1 below.
Using a treadmill (Muromachi Kikai MK-680), the gradient was set to 5 degrees only on the 5th day, but on the 1st to 4th days, the vehicle was forced to run with a gradient of 0 degrees.

  After the training, using a treadmill (Muromachi Kikai MK-680), the gradient was 5 degrees and the running time was 3 hours, and the limit running time was measured with the running speed time program described in Table 2 below. The groups were divided so that the average value of the critical travel time between the groups was almost the same. The number of n in one group was 6.

From the 7th day after the start of the test, Be2 was orally administered once a day with a sonde at a weight (kg) of 250 mg / mouse. Be2 used was dissolved in distilled water of 5 mL / mouse body weight (kg).
During the administration period, training was performed every 1-2 days for 30 minutes according to the running speed time program on the second day of Table 1 above.

  After oral administration of Be2 with a sonde 16 days after the third week (17th day), a treadmill (Muromachi Kikai MK-680) was used, the gradient was 5 degrees and the running time was 3 hours. The limit running time (seconds) was measured using the running speed time program described in 1. As a measurement result, the average value of each group is shown in Table 3 and FIG. Table 4 and FIG. 2 show the extension time (seconds) of the limit travel time.

  The limit running time (seconds) and the extension time (seconds) of the limit running time were measured by using only distilled water orally administered with a sonde at 5 mL / mouse body weight (kg) as a control group.

  From the results of FIG. 1 and FIG. 2, the effect of extending the limit travel time was observed in the group administered with the morohea extract of Production Example 2.

[Example 2]
(Measurement of limit travel time by treadmill)
A group to which Ae2 obtained by the method of Production Example 1 was administered was a Morohaya extract AMP group, a group to which Be2 obtained by the method of Production Example 2 was administered was a Morohaya extract MP group, and a group to which distilled water was administered As a control group, the following experiment was conducted.
In the same manner as in Example 1, after training, the limit travel time was measured, and the groups were divided so that the average value of the limit travel time between the groups was substantially the same. For the control group and the MP group, the n number of one group was 7, and for the AMP group, the n number was 8.

From the seventh day after the start of the test, Ae2 and Be2 were orally administered once a day with a sonde at a weight of 250 mg / mouse (kg).
Ae2 and Be2 were dissolved in 5 mL / mouse body weight (kg) of distilled water.
During the administration period, training was performed every 1-2 days for 30 minutes according to the running speed time program on the second day of Table 1 above.

  After 16 days after the third week (17th day), Ae2 and Be2 were orally administered by a sonde, respectively, and then the limit running time (seconds) was measured with the running speed time program shown in Table 2 above. As a measurement result, the average value of each group is shown in Table 5 and FIG. Table 6 and FIG. 4 show the extension time (seconds) of the limit travel time.

  The limit running time (seconds) and the extension time (seconds) of the limit running time were measured by using only distilled water orally administered with a sonde at 5 mL / mouse body weight (kg) as a control group.

  From the results of FIGS. 3 and 4, in the group administered with the morohea extract of Production Example 2, an effect of extending the limit travel time was observed, but in the morohea extract not subjected to the enzyme treatment of Production Example 1, The effect of extending the limit travel time was not observed.

  The morohea extract of the present invention has an anti-fatigue effect and a physical strength improving effect. The moroheiya extract of the present invention has industrial applicability as an anti-fatigue agent and a physical strength improver for pharmaceuticals and foods.

Claims (5)

  An anti-fatigue agent or a physical strength improver containing a moroheiya extract obtained by enzyme treatment.   The anti-fatigue agent or physical fitness improver according to claim 1, wherein the Morohaya extract contains a Morohaya synthetic resin adsorption fraction.   The anti-fatigue agent or physical fitness improver according to claim 1 or 2, wherein the enzyme is a protease.   The composition for anti-fatigue or a physical strength improvement containing the anti-fatigue agent or physical strength improver of any one of Claims 1-3.   The anti-fatigue or the physical fitness improvement food-drinks containing the anti-fatigue agent or physical strength improvement agent of any one of Claims 1-3.