JP2010241738A - Pest attractant and pest catcher having the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an insect pest attractant containing a substance derived from a genuine Myxomycetes belonging to the genus Physarum as an active ingredient, and an insect pest-capturing tool having the same. <P>SOLUTION: This insect pest attractant can exhibit a potent insect pest-attracting effect by using the substance originated from the genuine Myxomycetes belonging to the genus Physarum, and also can widely attract a plurality of kinds of insect pests even by using it as a single agent. In the case that the genuine Myxomycetes belonging to the genus Physarum is Physarum polycephalum, it is possible to exhibit a further potent insect pest-attracting effect, and also further widely attract the plurality of kinds of insect pests. Also, the handling of the same such as transportation becomes easier since its life cycle is in a plasmodium or sclerotium state. Further, by blending an insecticidal component to the active ingredient, the attracted insect pest can be controlled by the insecticidal component, and the efficient attraction/control of the insect pest becomes possible. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a pest attractant containing as an active ingredient a substance derived from the genus Physalum spp. And a pest trap having the pest, and more specifically, (A) a Psalm spp. And (C) the body of Phisalum true slime mold or mixture of crushed material and solvent, and a pest attractant containing as an active ingredient a substance selected from the group of (A) to (C) It relates to pest traps.

  Pests refer to insects that have harmful effects on humans, livestock, agricultural products, and the like. For example, sanitary pests such as cockroaches and flies mechanically carry pathogenic bacteria, and agricultural pests such as slugs eat and eat various types of plant leaves and fruits.

  Conventionally, in order to capture and control these pests, a method for attracting and capturing the pests using a pest attractant has been provided. The method for capturing and extinguishing pests using this pest attractant is simple and easy because there is no need to track pests, and when pesticides are added to pest attractants, it is not necessary to administer pesticides directly to pests. It is known as an effective method.

  Today, various types of pest attractants are known. As such pest attracting substances, for example, potatoes, onions, okaras, rice bran and various fatty acids are known for cockroaches, etc., and for crickets such as crickets, grasshoppers, praying mantis, mantis Vanillin, piperol, etc. are known.

  However, the conventional pest attractant may be (a) the pest-inducing effect may be insufficient, or (b) a single type of pest attractant may not be able to attract a wide variety of pests. There is an inconvenience. Further, in recent years, techniques for separating and extracting a pest attracting substance and specifying the chemical substance to some extent are known (for example, Japanese Patent Laid-Open Nos. 6-24923, 6-172104, and 9). No. 169611, etc.), (c) It is difficult to identify, separate and extract chemical substances having an attracting action, and there is a disadvantage that the burden of production cost is large.

JP-A-6-24923 JP-A-6-172104 JP-A-9-169611

  The present invention has been made in view of these disadvantages, has a strong pest attracting action, can attract a wide variety of pests, and can be produced at low cost, and a pest having the pest The purpose is to provide a trap.

The invention made in order to solve the said subject is the insect pest attractant which uses the substance derived from a Physalum genus slime mold as an active ingredient. In particular,
(A) Phisalum true slime mold body (B) Phisalum true slime mold crushed material (C) Phisalum true slime mold body or mixture of crushed material and solvent selected from groups (A) to (C) It is a pest attractant containing the substance to be used as an active ingredient.

  Thus, by using a substance derived from the genus physalum true slime mold as an active ingredient, it is possible to exert a powerful pest attracting effect, and even if the pest attractant is used as a single agent, a plurality of kinds of pests Can be widely attracted. Moreover, this physalum genus slime mold is good in it being a physalum polycephalum (Physarum Polycephalum). Thereby, a more powerful pest-inducing effect can be exhibited, and a plurality of types of pests can be attracted more widely.

  In addition, since the above-mentioned physalum genus slime mold is used in a deformed form or sclerotia in the life cycle, handling of the physalum genus slime mold is easy, such as transportation, crushing work, and storage. Furthermore, by incorporating an insecticidal component into the active ingredient, the attracted pest can be controlled by the insecticidal component, and it is possible to efficiently attract and control the pest.

  Moreover, another invention made | formed in order to solve the said subject is a pest capturing tool which has the said pest attractant. Like the pest attractant, this pest trap does not need to identify, separate and extract chemical substances that have a pest attracting effect, exerts a powerful pest-inducing effect, and attracts multiple types of pests widely.・ Can be captured.

  Here, “pests” refer to organisms that have harmful effects on humans, livestock, agricultural products, and the like. Specifically, it refers to agricultural pests, stored grain pests, sanitary pests, food pests, property pests, livestock pests, and unpleasant pests.

  As described above, the pest attractant of the present invention and the pest trapping tool having the pest attractant include (A) Physalum spp., (B) Psalm spp., (C) Psalm spp. By using a substance selected from the three groups of the fungus body or a mixture of crushed material and a solvent as an active ingredient, it is not necessary to specifically identify, separate and extract chemical substances having an attracting action. The burden can be reduced. In addition, even if this physalum genus slime mold is physalum polycephalum, the same cost reduction can be realized.

  Moreover, handling such as transportation, crushing work, and storage of the active ingredient is facilitated by using the physalum genus slime mold in a deformed form in the life cycle or in the state of mycorrhiza. Furthermore, by blending the insecticidal component, the attracted pest can be controlled by the insecticidal component, and it is possible to efficiently attract and control the pest.

  As a result, according to the pest attractant and the pest catcher having the pest attractant, there are cases where (a) the pest inducement effect is insufficient in the conventional pest attractant, (b) against one kind of pest attractant In some cases, it is not possible to attract a wide variety of pests. (C) It is difficult to identify, separate and extract chemical substances having an attracting action, and the disadvantage of high production cost is eliminated. it can.

The figure showing the result of the experiment 1 in an Example The figure showing the result of the experiment 2 in an Example

  Hereinafter, embodiments of the present invention will be described in detail.

  The pest attractant of the present invention and the pest trapping tool having the pest attractant contain a substance derived from a physalum genus slime mold as an active ingredient. Specifically, it is selected from the following three groups: (A) Phisalum true slime mold body, (B) Phisalum true slime mold crushed material, (C) Phisalum true slime mold body or mixture of crushed material and solvent It is a substance to be used.

  Phisalum true slime mold has a unique life cycle. Specifically, spores released from fruiting bodies germinate at a suitable place such as a rotting tree and are released as monolayer (n) amoeba cells. This amoeba-like cell supplements microorganisms and proliferates by dividing into two, while also functioning as a gamete. It joins with cells of different sex to form a multilayer (2n) amoeba. This multi-layered amoeba feeds on microorganisms, repeats fission without cell division and cell growth, and grows into a multinucleated variant with countless nuclei.

  This deformed body grows by phagocytosis of microorganisms, but even if the nucleus divides, the deformed body itself does not divide and gradually becomes one large cell mass containing many nuclei. Such a deformed body often hides in a decayed tree or soil, and forms a mycorrhizal diapause during drying. And when it comes to an appropriate time, it comes out on the surface of the earth and is divided into parts of about several millimeters, and each becomes a small child entity. During the formation of this fruiting body, the nucleus of the deformed body undergoes meiosis, and the protoplasm is divided around this nucleus to produce a monolayer of spores.

  It is known that the physalum genus slime mold has a unique fragrance in the entire cycle of the life cycle described above. Such a unique fragrance has the property of attracting strong insects such as cockroaches and flies, and has the property of attracting a wide variety of insect pests. That is, the pest attractant is not specifically specified, but utilizes a unique component possessed by such a physalum slime mold.

  Here, the pests include, for example, sanitary pests such as flies, mosquitoes, cockroaches, ticks, fleas; agricultural pests such as aphids, slugs, white fowl mites, thrips, etc .; bakuga, weevil, horned moth, Stored grain pests; clothing pests such as cutworms, moths, and moths.

  The physalum slime mold body has the above-mentioned effects sufficiently not only in a alive state but also in a dry or frozen state. Moreover, this crushed material can be efficiently obtained by a homogenizer, a mixer, or the like, and even a mixture obtained by dissolving the crushed material in a solvent has the above-described effects sufficiently.

  Examples of the solvent include distilled water, physiological saline, phosphate buffer, methanol, ethanol, propanol, isopropanol, 1,3-butylene glycol, propylene glycol, dipropylene glycol, glycerin, ethyl ether, propyl ether, and acetic acid. Examples include ethyl, butyl acetate, acetone, and ethyl methyl ketone.

  It is preferable that the physalum slime mold is physalum polycephalum. This physalum polycephalum has a fragrance similar to spermine or a plant-derived phytoncide, can exert a strong pest-inducing action, and can attract a wide variety of pests. Moreover, since it is widely used in various cell biological experiments and can be easily obtained, cost reduction can be realized.

  Here, the pest attracting action can be enhanced by a synergistic effect by blending an arbitrary pest attracting substance with the pest attractant. This optional pest attractant includes animal proteins, vegetable proteins, carbohydrates, vegetable oils, animal oils, fatty acid methyl or ethyl esters, saturated or unsaturated alcohols, non-reducing starch hydrolysates, cyclohexyl groups. Carboxylic acid ester, aromatic fatty acid ketone, alcohol, aldehyde, lactone, ester, aloe or eucalyptus plant, C 7-9 ketone, vanillin, ethyl vanillin, isovanillin, piperonal, piperonal acetone, 2,6- Nonadiether and the like can be mentioned.

  Examples of the animal protein include actin, albumin, casein, fibrin, fibrinogen, keratin, globulin (α, β, γ), hemoglobin, myosin, etc., locust, grasshopper, mantis, cricket, bee, aphid , Cockroaches, butterflies, moths, flies, eggs, fish meal, willows, krill, shrimp, milk, cheese, feed yeast, beef, horse meat and the like.

  Examples of the plant protein include edestine, zein, gliadin, allatin, zein, gluten, and the like, and starch flour, wheat flour, rice flour, bran, soybean, cottonseed, rapeseed, sesame seeds, rice bran, millet, potato, Sweet potatoes, corn, onions, bananas, peanuts, pumpkin seeds, broad beans and the like.

  Examples of the carbohydrates include fruit juice, honey, molasses, sugar cane, palatinose, α, α-trehalose, β, β-trehalose, α, β-trehalose, sophorose, tullanose, laminaribiose, nigerose, cellobiose, xylobiose, Leucrose, gentiobiose, melbiose, lutinose, primbelose, vicyanose, robinose, N-acetylglucosamine, galacturonic acid, mannose, xylose, arabinose, glucuronic acid, glucosamine, sucrose, glucose, fructose, lactose, brown sugar, red sugar, tri-warm Examples include sugar, granulated sugar, arabinose, galactose, sorbitol, glycerin and the like.

  Examples of the vegetable oil include tree wax, coconut oil, cacao oil, castor oil, olive oil, peanut oil, linseed oil, tung oil, hemp seed oil, and eno oil sugar. Examples of animal oils include body fat, butter fat, leg fat, whale oil, dolphin oil, sardine oil, herring oil, cod liver oil, shark liver oil, squid oil, clam oil, and egg oil sugar.

  It is good to use a physalum-genus slime mold in the state of the deformed body in a life cycle, or a sclerotia. This deformation body generally has a size of several centimeters to several tens of centimeters and a thickness of about several millimeters, and has a mesh-like flat structure. In addition, the sclerotia can survive for several years or more in a dry state, and returns to the deformed body when moisture is applied. Therefore, handling such as transportation, crushing work and storage becomes easy.

  The active ingredient may be further mixed with an insecticidal ingredient. As a result, the pests are attracted by the powerful attracting action of the genus Plasmodium spp., And the pests attracted by the insecticidal ingredients blended with the above active ingredients are also extinguished. Can be done automatically. Examples of such insecticidal components include pyrethroid insecticides, organophosphorus insecticides, carbamate insecticides, 5-methoxy-3- (O-methoxyphenyl) -1,3,4-oxydiazole-2 (3H ) -One, 1- (6-chloro-3-pyridylmethyl) -N-nitroimidazolidin-2-ylideneamine, and the like.

  Examples of the pyrethroid insecticide include dl-3-allyl-2-methyl-4-oxo-2-cyclopentenyl-dl-cis / trans-chrysanthemate, dl-3-allyl-2-methyl-4 -Oxo-2-cyclopentenyl-d-cis / trans-chrysanthemate, dl-3-allyl-2-methyl-4-oxo-2-cyclopentenyl-d-trans-chrysanthemate, d-3-allyl -2-methyl-4-oxo-2-cyclopentenyl-d-trans-chrysanthemate, (1,3,4,5,6,7-hexahydro-1,3-dioxo-2-indolyl) methyl-dl Cis / trans-chrysanthemate, (1,3,4,5,6,7-hexahydro-1,3-dioxo-2-indolyl) methyl-d-cis / trans-cry Nantemate, (5-benzyl-3-furyl) methyl-d-cis / trans-chrysantemate, 3-phenoxybenzyl-dl-cis / trans-3- (2,2-dichlorovinyl) -2,2-dimethyl -1-cyclopropanecarboxylate, 3-phenoxybenzyl-d-cis / trans-chrysantemate, α-cyano-3-phenoxybenzyl-2- (4-chlorophenyl) -3-methylbutyrate, α-cyano- 3-phenoxybenzyl-cis / trans-2,2,3,3-tetramethylcyclopropanecarboxylate, 1-ethynyl-2-methyl-2-pentenyl-dl-cis / trans-chrysanthemate, 2,3 4,5,6-pentafluorobenzyl-dl-cis / trans-3- (2,2-dichlorovinyl) -2,2- Methyl-1-cyclopropanecarboxylate, 1-ethynyl-2-methyl-2-pentenyl-dl-cis / trans-3- (2,2-dichlorovinyl) -2,2-dimethyl-1-cyclopropanecarboxylate 1-ethynyl-2-methyl-2-pentenyl-cis / trans-2,2,3,3-tetramethyl-1-cyclopropanecarboxylate, (+)-2-methyl-4-oxo-3- ( 2-propynyl) -2-cyclopentenyl (+)-cis / trans-chrysanthemate, d-trans-2,3,5,6-tetrafluorobenzyl-3- (2,2-dichlorovinyl) -2, 2-dimethyl-1-cyclopropanecarboxylate, 2,3,5,6-tetrafluoro-4-methylbenzyl-3- (2-chloro-3,3,3-trif Oro-1-propenyl) -2,2-dimethyl-1-cyclopropanecarboxylate, (±) α-cyano-3-phenoxybenzyl (+)-cis / trans-chrysanthemate, dl-3-allyl-2 -Methyl-4-oxo-2-cyclopentenyl-dl-cis / trans-2,2,3,3-tetramethylcyclopropanecarboxylate, 2- (4-ethoxyphenyl) -2-methylpropyl-3-phenoxy Examples thereof include benzyl ether.

  Examples of the organophosphorus insecticide include O, O-dimethyl-O- (2,2-dichloro) vinyl phosphate, O, O-dimethyl-O- (3-methyl-4-nitrophenyl) thiono. Phosphate, O, O-diethyl-O-2-isopropyl-4-methyl-pyrimidyl- (6) -thiophosphate, O, O-dimethyl-S- (1,2-dicarboethoxyethyl) -dithiophosphate , O, S-dimethylacetylphosphoroamidothioate and the like.

  Examples of the carbamate insecticide include O-isopropoxyphenyl methyl carbamate, 1-naphthyl-N-methyl carbamate, ethyl N- [2,3-dihydro-2,2-dimethylbenzofuran-7-yloxycarbonyl. (Methyl) aminothio] -N-isopropyl-β-alaninate, ethyl-N-benzyl-N-{[methylthio (1-ethylidene-amino-oxycarbonyl) amino] thio} -β-alaninate, ethyl-2- (4 -Phenoxyphenoxy) ethyl carbamate and the like.

  The dosage form of the pest attractant is not particularly limited, and for example, it can be used in dosage forms such as aerosols and smoke agents. In addition, it can be applied, sprayed, printed, etc. in a place considered to be the range of action of pests.

  In addition, the said pest attractant is not limited to the said embodiment, For example, the pest trap which has the said pest attractant can be provided. There is no particular limitation on the shape or the like of the pest capturing tool.

  EXAMPLES Hereinafter, although this invention is explained in full detail based on an Example, this invention is not interpreted limitedly based on description of this Example.

<Test pest>
As test insects, German cockroaches and black cockroaches (Fumakiller Co., Ltd.) were used. This German cockroach and black cockroach are not distinguished from each other.

[Examples 1-2]
<Culture of true slime mold and adjustment of active ingredient>
Glucose 5 g, yeast extract 0.5 g, bactopeptone 5 g, potassium dihydrogen phosphate 2.3 g, dipotassium hydrogen phosphate 1.5 g, magnesium sulfate heptahydrate 0.5 g, and agar 30 g were prepared with 1 liter of distilled water. After sterilization, it was dispensed into a petri dish to prepare an agar medium having a thickness of 0.7 cm. After inoculating a deformed form of Fisarum polycephalum on this agar medium, oatmeal was added, and the deformed body was grown by culturing in a 25 ° C. furan apparatus for 3 days.

  Thereafter, the proliferated deformed body was scraped, and 1 ml and 2 g (all wet weights) of each were added with 1 ml of distilled water and crushed as an active ingredient. And the thing of the deformation body 1g was made into Example 1, and the thing of the deformation body 2g was made into Example 2. FIG.

[Comparative Example 1]
As a comparative example, a cockroach attractant (earth pharmaceutical) comprising beef, shrimp, potato and the like was prepared.

[Characteristic evaluation]
<Experimental system settings>
In this attraction experiment, a plastic clothing box (44 cm long, 66 cm wide, 32 cm high) with Japanese paper spread on the bottom and powder talc applied to the inside of the wall was set as the experimental system. A paper towel containing water and a wooden shelter were installed near the inner center of the costume box. On the day before the attraction experiment, 10 German cockroaches (Experiment 1) or 10 black cockroaches (Experiment 2) were released in the costume box and left overnight in a starved state.

  Next, a transparent cylindrical polycup (diameter 13 cm, height 9 cm) was prepared, and the entrance / exit of the test insect pest (height 5 cm, width 8 cm) was cut out. Powder talc is applied to the outer and inner walls of the polycup. Thereafter, the following treatments (i) to (iii) were performed on the polycup.

(I) A paper towel infiltrated with the active ingredient of Example 1 (deformation body 1g) was attached to the center of the inner upper surface of the polycup with an adhesive tape.
(Ii) A paper towel infiltrated with the active ingredient of Example 2 (deformation body 2g) was attached to the center of the inner upper surface of the polycup with an adhesive tape.
(Iii) The cockroach attractant of Comparative Example 1 was attached to the center of the inner upper surface of the polycup with an adhesive tape.

  The pest attracting activity in this attracting experiment was evaluated by installing the polycup in a costume box.

<Evaluation method>
The pest attracting activity was evaluated by placing the polycups (i) to (iii) in a costume box and counting the number of times the cockroach entered each polycup.

  Regarding the counting method, the time when the polycup was placed was set to 0 minute, and the number of cockroaches that entered each polycup was counted every minute for 15 minutes. When the same individual invaded the same polycup several times during the same 1 minute interval, the number of invading individuals was counted as one. For example, if the same individual invades the same polycup twice during 3 to 4 minutes, the count is one.

  However, when the same individual invaded the same polycup multiple times at different intervals, it was counted as multiple intrusions. For example, when an individual that has entered a polycup in an interval of 3 to 4 minutes enters the same polycup again in an interval of 4 to 5 minutes, the count is two.

<Experiment 1>
The polycups (i) and (iii) were placed in a costume box from which 10 German cockroaches were released, and the pest attracting activity was evaluated. All external light was blocked, and all the experimental steps were performed under the condition of 24 ° C. under red light irradiation.

<Experiment 2>
The polycups (ii) and (iii) were placed in a costume box from which 10 black cockroaches were released, and the pest attracting activity was evaluated. All external light was blocked, and all the experimental steps were performed under the condition of 24 ° C. under red light irradiation.

<Evaluation of attracting action>
The result of Experiment 1 is shown in FIG. 1, and the result of Experiment 2 is shown in FIG. From FIG. 1 and FIG. 2, it was found that the pest attractant of Example 1 and Example 2 showed a stronger pest inducer effect than Comparative Example 1 regardless of the type of black cockroach and German cockroach.

  As described above, the pest attractant of the present invention and the pest trapping tool having the pest attractant include a substance derived from a physalum genus slime mold as an active ingredient, exhibiting a strong pest attracting effect, Can attract pests extensively. Furthermore, it is not necessary to identify, separate and extract chemical substances that have an attracting action, and it is possible to reduce costs by facilitating propagation using waste grains, etc. Widely used in

Claims (6)

  A pest attractant containing as an active ingredient a substance derived from the genus Physarum true slime mold. A substance derived from the above genus slime mold,
(A) Phisalum true slime mold body (B) Phisalum true slime mold crushed material (C) Phisalum true slime mold body or mixture of crushed material and solvent selected from the group of (A) to (C) The pest attractant according to claim 1.   The insect pest attractant according to claim 1 or 2, wherein the true genus slime mold is Physalum polycephalum.   The insect pest attractant according to claim 1, wherein the physalum genus slime mold is used in a deformed state or a mycorrhizal state in a life cycle.   The pest attractant according to any one of claims 1 to 4, wherein an insecticidal component is further added to the active ingredient.   A pest capturing tool comprising the pest attractant according to any one of claims 1 to 5.

Images