JP2010008191A - Reaction vessel plate and reaction treatment method - Google Patents

Abstract

An object of the present invention is to prevent entry of foreign matter from the outside of a reaction vessel plate and environmental pollution to the outside.
A base plate 3 includes a reaction container 5, each flow path 13, 15, 17, 19 connected to the reaction container 5, and a sample container flow path 35a connected to a sample container 35. One end of each flow path is provided. Is pulled out to the bottom. A channel drawing plate 55 is attached to the lower surface of the base plate 3 via a seal plate 54. Each flow path port connected to each flow path 13, 15, 17, 19, 35 a is provided on the lower surface of the flow path drawing plate 55. The reaction container 5, the flow paths 13, 15, 17, and 19, the sample container 35, and the sample container flow path 35a form a closed system. A rotary switching valve 87 is attached to the reaction vessel plate 1. The rotary switching valve 87 includes a syringe for sucking and discharging liquid or gas.
[Selection] Figure 1

Description

  INDUSTRIAL APPLICABILITY The present invention relates to a reaction vessel plate suitable for performing various analyzes and analyzes in the field of medical analysis and chemistry in the field of biological analysis, biochemical analysis, or chemical analysis in general, and a method for processing the reaction vessel plate. The present invention relates to a reaction processing method.

A micro multi-chamber apparatus is used as a small reaction apparatus used for biochemical analysis or normal chemical analysis. As such an apparatus, for example, a microwell reaction vessel plate such as a microtiter base plate in which a plurality of wells are formed on a flat substrate surface is used (see, for example, Patent Document 1).
Moreover, as a trace liquid weighing structure capable of quantitatively handling a trace amount of liquid, a first channel and a second channel, a third channel opening in the channel wall of the first channel, A structure having a fourth channel having a property of opening the channel wall of the two channels and connecting one end of the third channel and the second channel so that capillary attraction is relatively difficult to work than the third channel. Some are provided (see, for example, Patent Documents 2 and 3). According to the trace liquid weighing structure, after the liquid introduced into the first flow path is drawn into the third flow path, the liquid remaining in the first flow path is removed, and the volume of the third flow path is increased. A corresponding volume of liquid can be weighed into the second flow path.
JP-A-2005-177749 JP 2004-163104 A JP 2005-114430 A Japanese Patent No. 3454717

When the conventional microwell reaction container plate is used, the upper surface of the reaction container plate is open to the atmosphere. Therefore, foreign matter may enter the sample from the outside, and conversely, the reaction product may contaminate the external environment.
Further, in the trace liquid weighing structure disclosed in Patent Documents 2 and 3, liquid introduction ports are formed at both ends of the first flow path and at both ends of the second flow path. It is possible that the reaction products are open and pollute the external environment through these ports.
Therefore, the present invention has an object to provide a reaction vessel plate that can prevent entry of foreign matter from the outside of the reaction vessel plate and environmental pollution to the outside, and a reaction processing method using the reaction vessel plate. It is.

  A reaction container plate according to the present invention includes a reaction container, a reaction container channel connected to the reaction container, a sealing container provided separately from the reaction container, and a sealing container channel connected to the sealing container. A base plate and a pull-out channel attached to the base plate and with one end connected to the reaction vessel channel and the sealing vessel channel to pull out the reaction vessel channel and the sealing vessel channel, respectively, and opposite to the base plate A flow path port arrangement portion on the side surface, and a flow path port in which the other end of each drawer flow channel is connected to the reaction vessel flow channel and the sealing vessel flow channel, respectively. Syringe port connected to a syringe with a drawer plate and a syringe that is rotatably attached to the channel drawer plate and that slides a plunger to suck and discharge liquid or gas. The syringe port placement part where the fluid is placed is provided at a position facing the flow path port placement part, and the syringe port placement part is in contact with the flow path port placement part so that the syringe port can be switched and connected to each flow path port. A rotary switching valve, and a seal plate made of an elastic material provided between the base plate and the flow channel extraction plate, and the reaction vessel, the reaction vessel flow channel, the sealing vessel, and the sealing vessel flow channel are hermetically sealed The edge of the channel on the surface of the base plate in contact with the seal plate or the edge of the channel on the surface of the channel drawing plate is raised.

  According to the above reaction container plate, each flow connected to the reaction container flow path and the sealing container flow path on the reaction container forming the closed system, the reaction container flow path, the sealing container, and the base plate provided with the sealing container flow path. A flow path drawing plate equipped with a flow path port arrangement part where a path port is arranged is mounted, and furthermore, a syringe that sucks and discharges liquid or gas is provided, and a syringe port connected to the syringe is selected as each flow path port Since the rotary type switching valve that can be connected in a rotating manner is rotatably mounted, the reaction vessel channel and the sealed vessel flow are not drawn out to the outside of the reaction vessel plate. The liquid can be sent through the channel, preventing foreign substances from entering the reaction container plate, and environmental pollution caused by liquid scattering from the reaction container plate to the outside. It can be prevented.

  A seal plate is sandwiched between the base plate and the drawer plate to improve the liquid tightness of the reaction vessel channel and the sealing vessel channel of the base plate and the drawer channel of the channel drawer plate. Furthermore, the edge of the flow path on the surface of the base plate in contact with the seal plate or the edge of the flow path on the surface of the flow path extraction plate in contact with the seal plate is raised, and the surface pressure of the edge of the flow path to be sealed by the seal plate is further increased. It is increasing. Thereby, the liquid tightness of the flow path between the base plate and the seal plate or the flow path between the flow path extraction plate and the seal plate is further improved.

  In addition, the sealing performance at the contact portion between the syringe port placement portion and the flow path port placement portion is also important. If the sealability of this portion is poor when trying to circulate the liquid using a syringe, liquid leakage occurs and accurate liquid feeding cannot be performed. In order to solve this problem, a member for pressing the syringe port placement portion of the rotary switching valve against the flow path port placement portion may be provided.

  For example, a fixed member engaging portion protruding from the surface of the flow channel drawer plate is provided around the flow channel port arrangement portion of the flow channel drawer plate, and the syringe port arranging portion of the rotary switching valve is provided inside the fixed member engaging portion. A ring-shaped lower surface side fixing member that contacts the peripheral edge of the opposite surface and engages with the fixing member engaging portion and presses the syringe port arrangement portion against the flow path port arrangement portion may be attached. If it does so, the sealing performance of the contact surface of a flow-path port arrangement | positioning part and a syringe port arrangement | positioning part will improve.

  In the following description, the surface opposite to the syringe port arrangement surface of the rotary switching valve is referred to as a “lower surface”, and the opposite surface, that is, the surface on the syringe port arrangement surface side is referred to as an “upper surface”. Similarly, for the base plate and other members, the surface on the same side as the “lower surface” of the rotary switching valve is defined as the “lower surface”, and the surface on the opposite side is defined as the “upper surface”.

  As a method for attaching the lower surface side fixing member, a method in which the lower surface side fixing member is pushed into the inside of the fixing member engaging portion from the lower surface side of the rotary switching valve can be considered. In such a case, the lower surface side fixing member is made of resin and the outer diameter thereof is substantially the same as the inner diameter of the fixing member engaging portion, and the outer peripheral surface of the lower surface side fixing member and the inner periphery of the fixing member engaging portion. First engaging structure portions that engage with each other at corresponding positions on the surface are provided, and the first engaging structure portion is an outer peripheral surface side surface of the lower surface side fixing member or an inner peripheral surface side surface of the fixing member engaging portion. It is preferable that any of these is inclined in the direction of closing to the base plate side. Then, when the lower surface side fixing member is pushed into the inside of the fixing member engaging portion, the fixing member engaging portion is arbitrarily elastically deformed along the inclination of the first engaging structure portion. Easy to install. “The outer diameter of the lower surface side fixing member is substantially the same as the inner diameter of the fixing member engaging portion” means that the outer diameter of the lower surface side fixing member is the same as the inner diameter of the fixing member engaging portion. The case where the outer diameter of the lower surface side fixing member is slightly smaller than the inner diameter of the fixing member engaging portion is also included. In addition, “inclination” here includes not only a linear inclination but also a case of inclination while curving, and the following “inclination” is also agreed.

  Further, the base plate and the flow path drawing plate have a through hole for inserting a part of the rotary type switching valve, and the rotary type switching valve has an insertion portion in which a part is inserted into the through hole from the flow path drawing plate side. And a non-insertion portion that is not inserted into the through hole with a diameter larger than that of the insertion portion and is in contact with the surface of the flow passage extraction plate around the through hole. The periphery of the through hole is the flow path port arrangement part, the position facing the flow port arrangement part of the non-insertion part of the rotary type switching valve is the syringe port arrangement part, and the insertion part tip of the rotary type switching valve is at the base plate It is a protrusion that passes through the through hole and protrudes to the opposite side of the base plate. On the outer peripheral surface of the protrusion, on the surface of the base plate around the through hole Touch while also engage the overhang outer peripheral surface may be tip-side fixing member for pulling the insertion portion in the penetrating direction is fitted with locking the rotary switching valve. Then, since the insertion portion of the rotary switching valve is pulled in the penetration direction by the distal end side fixing member, the syringe port arrangement portion provided around the insertion portion base end portion on the upper surface of the non-insertion portion is accordingly accompanied. It is pressed against the channel port arrangement part side, and the sealing performance is enhanced.

  The difference between the case where the distal end side fixing member is attached and the case where the lower surface side fixing member is attached is that when the lower surface side fixing member is attached, the peripheral edge side of the syringe port arrangement portion is pressed against the flow path port arrangement portion side. On the other hand, when the distal end side fixing member is attached, the center side of the syringe port arrangement portion is pressed against the flow path port arrangement portion side. The rotary type switching valve can be fixed by only one of the lower surface side fixing member and the front end side fixing member, but by attaching both of these fixing members, the peripheral side and the central side of the syringe port arrangement portion Since the force applied to the syringe port arrangement part becomes more uniform in the surface, the sealing performance can be further improved.

  In addition, as a front end side fixing member, the ring-shaped or cap-shaped member of the system inserted from the insertion part front-end | tip of a rotary type switching valve can be considered. In such a case, the distal-end-side fixing member is made of resin and the inner diameter thereof is substantially the same as the outer shape of the protruding portion. A second engaging structure portion is provided, and the second engaging structure portion is inclined in a direction in which the inner peripheral surface side surface of the distal end side fixing member or the outer peripheral surface side surface of the projecting portion is closed toward the distal end side of the projecting portion. Preferably it is. Then, the tip side fixing member in the form of a ring or cap is fitted to the tip of the insertion portion of the rotary switching valve and pushed toward the base end side, and the tip side is moved along the inclination of the second engagement structure portion. Since the fixing members are arbitrarily elastically deformed and engaged with each other at a predetermined position, the distal end side fixing member can be easily attached. Note that “the inner diameter of the tip-side fixing member is substantially the same as the outer diameter of the protruding portion” means that the inner diameter of the tip-side fixing member is the same as the outer diameter of the protruding portion, This includes the case where the inner diameter is slightly larger than the outer diameter of the protrusion.

  By the way, when a liquid such as a reagent or dilution water or a powdered solid such as a reagent is sealed in advance, if the sealed container is connected to the sealed container flow path, the reaction container can be used even when it is not used. There is a risk that liquid or solid may enter the sealed container flow path. Therefore, in the reaction container plate of the present invention, the sealed container flow path can be connected to the sealed container only at the time of use. In one example of such a structure, the bottom surface of the sealing container is provided with a penetrating portion that can be pressed through from the outside, and one end of the sealing container channel protrudes upward, and is sealed when used. The sealed container flow path is connected to the sealed container by one end of the stop container flow path passing through the penetrating portion. With this structure, the reaction container plate can be stored in a state where the sealed container and the sealed container flow path are separated. Therefore, even if the liquid is sealed in the sealed container in advance, the sealed container flow path is stored at the time of storage. It is possible to prevent evaporation of the liquid in the sealed container through the. And when the liquid previously enclosed with the sealing container is a reagent, concentration of the reagent can be prevented.

  In the above case, the sealing container is held so that the first penetration part faces one end of the sealing container channel when not in use, and one end of the sealing container channel penetrates the first penetration part when used. If it keeps in that state, it can be maintained and used in a state where the sealing container and the sealing container flow path are connected only by lowering the sealing container.

  Furthermore, a second penetrating portion that can be pressed from the outside and penetrated is provided at a position where the sealed container does not touch the liquid contained therein, and one end of the base plate body projects upward, You may further provide the sealing container air vent flow path which one end presses and penetrates the 2nd penetration part at the time of use of a sealing container. If it does so, in the case of the injection | pouring of the liquid to a sealing container, and the suction | inhalation of the liquid from a sealing container, gas can be distribute | circulated between a sealing container and a sealing container air vent flow path. Thereby, the injection | pouring of the liquid to a sealing container and the suction | inhalation of the liquid from a sealing container can be performed smoothly.

  The said sealing container can be used as a sample container for accommodating a sample liquid. This eliminates the need to prepare a separate container for containing the sample.

  When a sealed container is used as a sample container, one surface of the sample container can be penetrated by a member having a pointed portion, and a seal made of an elastic member that can repair a hole formed there after the drawing of the penetrated member by an elastic force It is preferably sealed with a member. If it does so, a sample liquid can be inject | poured in a sample container via a sealing member with the injection device with a pointed part, and it can prevent that a sample liquid leaks from the sample container after extraction of an injection device.

  Moreover, the sample pretreatment liquid or the reagent may be previously stored in the sealing container. This eliminates the need to dispense the sample pretreatment liquid or reagent into the sample container.

  The sealed container can also be used as a gene amplification container for performing a gene amplification reaction. Then, even in a sample solution containing only a very small amount of the gene to be measured, the analysis accuracy can be increased by amplifying the gene on the reaction vessel plate by a gene amplification reaction such as the PCR method or the LAMP method. In this case, the gene amplification container preferably has a shape suitable for temperature control at a predetermined temperature cycle. Note that the reaction vessel may be a gene amplification unit.

As a specific flow path configuration example of the reaction container plate of the present invention, the reaction container plate further includes a reaction container air vent flow path connected to the reaction container, and the reaction container flow path is formed on the bonding surface of two bonded members. A groove formed or a through-hole formed in the groove and the substrate and connected to the syringe; a metering channel having a predetermined capacity branched from the main channel; and one end of the metering channel An injection channel connected to the channel and connected at the other end to the reaction vessel, the main channel and the reaction vessel air vent channel can be sealed; In the liquid introduction pressure state when the liquid is introduced into the main flow path and the metering flow path and the purge pressure state when the liquid in the main flow path is purged, the liquid is not passed. Than under pressure Mention may be made of those passing through the serial liquid.
Here, “the injection channel is formed thinner than the metering channel” means that when the injection channel is composed of a plurality of channels, the plurality of channels constituting the injection channel are It means that each is formed narrower than the measuring channel.

  A reaction processing method using the reaction container plate according to the present invention is a reaction processing method using the reaction container plate of the present invention in the above-described flow path configuration example, and is applied to the main flow path and the measurement flow path with the introduction pressure. The liquid is filled, gas is allowed to flow in the main channel, and the liquid in the main channel is discharged while the liquid remains in the metering channel, and the main channel is set to a positive pressure greater than the introduction pressure. Alternatively, the liquid in the metering channel is injected into the reaction vessel through the injection channel by setting the inside of the reaction vessel to a negative pressure or both the positive pressure and the negative pressure.

In the flow channel configuration example, a contact angle of the injection flow channel with respect to water droplets is 90 degrees or more, and an area of a boundary between the injection flow channel and the measurement flow channel is 1 to 10000000 μm ² (square micrometer). preferable. Then, when the liquid is introduced into the main channel and the metering channel, the liquid is less likely to enter the injection channel, and the introduction pressure when introducing the liquid into the main channel and the metering channel can be increased. .
Here, when the injection flow path is constituted by a plurality of flow paths, the area means an area of a boundary between each of the plurality of flow paths constituting the injection flow path and the measurement flow path.

  A plurality of the reaction vessels may be provided, each of the reaction vessels may be provided with the metering channel and the injection channel, and the plurality of metering channels may be connected to the main channel. If it does so, a liquid can be sequentially introduce | transduced into a some measurement flow path, and a liquid can be simultaneously inject | poured into a several reaction container via an injection | pouring flow path after that.

  The other end of the injection channel is disposed at the tip of a convex portion that protrudes from the inner upper surface of the reaction vessel, and the tip of the convex portion is thinner than the base end portion. preferable. If it does so, the liquid inject | poured into a reaction container through an injection | throwing flow path will become easy to dripping at a reaction container.

When the reaction vessel plate of the present invention is used as a reaction vessel plate for measuring a sample containing a gene, a sample subjected to a gene amplification reaction in advance may be introduced into this reaction vessel plate, or this reaction The gene amplification reagent can be stored in advance or dispensed so that the reaction vessel on the container plate can perform the gene amplification reaction.
The gene amplification reaction includes a PCR method and a LAMP method. Focusing on the PCR method for amplifying DNA, a method of directly performing a PCR reaction from a sample such as blood without pretreatment has also been proposed. In the nucleic acid synthesis method for amplifying a target gene in a sample containing a gene, the gene inclusion body in the sample containing the gene or the sample containing the gene itself is added to the gene amplification reaction solution, The target gene in the sample containing the gene is amplified when the pH of the reaction solution is 8.5 to 9.5 (25 ° C.) (see Patent Document 4).

  The reaction vessel may be made of a light-transmitting material so that optical measurement can be performed from the bottom or above. Then, the liquid in the reaction vessel can be measured optically without moving to another vessel.

  In the reaction container plate of the present invention, a reaction container, reaction container flow path, a sealing container, and a drawer channel for drawing out the reaction container flow path and the sealing container flow path to a base plate having a sealing container flow path are formed. And a syringe for performing suction or discharge of liquid or gas, and selectively connecting the reaction vessel channel and the sealing vessel channel to the syringe. Since the rotary switching valve constituting the rotary switching valve is rotatably mounted, the reaction vessel channel and the sealing vessel flow are not drawn out to the outside of the reaction vessel plate. The liquid can be fed through the channel, preventing foreign substances from entering the reaction vessel plate from the outside of the flow channel, and the liquid from the reaction vessel plate to the outside. Environmental pollution can be prevented by eliminating the scattering.

1A and 1B are diagrams showing an embodiment of a reaction vessel plate. FIG. 1A is a schematic plan view, and FIG. 1B is a sectional view taken along a line AA in FIG. FIG. 2 is a schematic cross-sectional view of the reaction vessel air vent channels 19 and 21, the liquid drain space 29, the air drain space 31, and the bellows 53, and FIG. 2A is a sectional view showing a rotary switching valve 87 of the reaction vessel plate of the same embodiment, FIG. 2B is a perspective view of a rotor cap 89, and FIG. 2C is a perspective view of a backup ring 91.
FIG. 3 is a schematic view showing the vicinity of one reaction vessel of this embodiment, where (A) is a plan view, (B) is a perspective view, and (C) is a cross-sectional view. 4A and 4B are enlarged views of the sample container accommodating portion and the sample container. FIG. 4A is a plan view of the sample container accommodating portion, FIG. 4B is a cross-sectional view at the BB position in FIG. ) Is a plan view of the sample container, (D) is a cross-sectional view at the CC position in (C), (E) is a cross-sectional view in which the sample container is disposed in the sample container housing portion at the first holding position, and (F). FIG. 5 is a cross-sectional view in which a sample container is disposed in a sample container housing portion at a second holding position. 5A and 5B are enlarged views of the reagent container housing part and the reagent container. FIG. 5A is a plan view of the reagent container housing part, FIG. 5B is a cross-sectional view taken along the DD line in FIG. ) Is a plan view of the reagent container, (D) is a cross-sectional view at the EE position of (C), (E) is a cross-sectional view in which the reagent container is arranged in the reagent container housing portion at the first holding position, (F) FIG. 6 is a cross-sectional view in which the reagent container is disposed in the reagent container housing portion at the second holding position. 6A and 6B are enlarged views of the air suction container housing portion and the air suction container. FIG. 6A is a plan view of the air suction container housing portion, and FIG. 6B is the FF position in FIG. (C) is a plan view of the air suction container, (D) is a cross-sectional view at the GG position of (C), and (E) is a diagram showing the air suction container in the air suction container housing portion. FIG. 6F is a cross-sectional view in which the air suction container is disposed in the air suction container housing portion in the second holding position.
An embodiment of the reaction vessel plate will be described with reference to FIGS.

  The reaction vessel plate 1 includes a plurality of reaction vessels 5 having openings on one surface of the base plate 3. In this embodiment, 6 × 6 reaction vessels 5 are arranged in a staggered manner. A reagent 7 and wax 9 are accommodated in the reaction vessel 5.

  The material of the base plate 3 including the reaction vessel 5 is not particularly limited. However, when the reaction vessel plate 1 is used as disposable, it is preferable that there is a material available at a low cost. As such a material, for example, a resin material such as polypropylene and polycarbonate is preferable. When the substance in the reaction vessel 5 is detected by absorbance, fluorescence, chemiluminescence, bioluminescence or the like, it must be formed of a light transmissive resin so that optical detection can be performed from the bottom side. Is preferred. In particular, when performing fluorescence detection, the base plate 3 may be made of a material such as a resin having a low autofluorescence property (a property of generating less fluorescence from itself) and a light transmitting resin, such as polycarbonate. preferable. The base plate 3 has a thickness of 0.2 to 4.0 mm (millimeters), preferably 1.0 to 2.0 mm. From the viewpoint of low autofluorescence for fluorescence detection, the base plate 3 is preferably thinner.

  Referring to FIGS. 1 and 3, the flow path base 11 is disposed on the base plate 3 so as to cover the arrangement region of the reaction vessels 5. The channel base 11 is made of, for example, PDMS (polydimethylsiloxane) or silicone rubber. The thickness of the channel base 11 is, for example, 1.0 to 5.0 mm. The flow path base 11 has a groove on the joint surface with the base plate 3. A main channel 13, a metering channel 15, an injection channel 17, reaction vessel air vent channels 19 and 21, and drain space air vent channels 23 and 25 are formed by the groove and the surface of the base plate 3. The main channel 13, the metering channel 15 and the injection channel 17 constitute a reaction vessel channel. A concave portion 27 disposed on the reaction vessel 5 is also formed on the joint surface of the flow path base 11 with the base plate 3. In FIG. 1A and FIGS. 3A and 3B, only the groove and the concave portion of the flow path base 11 are illustrated.

  The main flow path 13 is composed of a single flow path and is formed to be bent so as to pass through the vicinity of all the reaction vessels 5. One end of the main flow path 13 is connected to a flow path 13 a formed of a through hole provided in the base plate 3. The flow path 13a is connected to a port of a rotary switching valve 87 described later. The other end of the main flow path 13 is connected to a liquid drain space 29 formed in the base plate 3. The dimensions of the grooves constituting the main flow path 13 are, for example, a depth of 400 μm (micrometer) and a width of 500 μm. In addition, the main flow path 13 has a predetermined length downstream of the position where the measurement flow path 15 is connected, for example, a 250 μm portion is formed to be narrower than other portions, for example, the width is 250 μm. It is.

  The measuring channel 15 is branched from the main channel 13 and provided for each reaction vessel 5. The end of the measuring channel 15 opposite to the main channel 13 is disposed in the vicinity of the reaction vessel 5. The depth of the groove constituting the measuring channel 15 is 400 μm, for example. The measuring channel 15 has an internal capacity of a predetermined capacity, for example, 2.5 μL (microliter). The width dimension of the part connected to the main flow path 13 of the measurement flow path 15 is larger than the narrow part of the main flow path 13 described above, for example, 500 μm. Thereby, the flow resistance of the main flow path 13 is larger than that of the measurement flow path 15 in the portion where the measurement flow path 15 is branched with respect to the liquid flowing from one end of the main flow path 13. The liquid flowing from one end of the main channel 13 first flows into the metering channel 15, and after the metering channel 15 is filled with the liquid, it flows downstream through the narrowed portion of the main channel 13. It has become.

An injection channel 17 is also provided for each reaction vessel 5. One end of the injection channel 17 is connected to the metering channel 15. The other end of the injection channel 17 is connected to a recess 27 disposed on the reaction vessel 5 and led to the reaction vessel 5. The injection channel 17 is formed with a dimension that maintains liquid tightness in the reaction vessel 5 in a state where there is no pressure difference between the reaction vessel 5 and the injection channel 17. In this embodiment, the injection channel 17 is composed of a plurality of grooves, and the dimensions of the grooves are, for example, a depth of 10 μm, a width of 20 μm, a pitch of 20 μm, and 13 grooves in a width region of 500 μm. Is formed. Here, the area of the boundary between the groove constituting the injection channel 17 and the metering channel 15, that is, the cross-sectional area of the groove constituting the injection channel 17 is 200 μm ² . The recess 27 has a depth of, for example, 400 μm, and the planar shape is a circle smaller than the reaction vessel 5.

  A reaction vessel air vent channel 19 is provided for each reaction vessel 5. One end of the reaction container air vent channel 19 is connected to a recess 27 disposed on the reaction container 5 at a position different from the injection channel 17 and disposed on the reaction container 5. The reaction container air vent channel 19 is formed with a dimension that maintains liquid tightness in the reaction container 5 in a state where there is no pressure difference between the reaction container 5 and the reaction container air vent channel 19. The other end of the reaction vessel air vent channel 19 is connected to the reaction vessel air vent channel 21. In this embodiment, the reaction vessel air vent channel 19 is composed of a plurality of grooves, and the dimensions of the grooves are, for example, a depth of 10 μm, a width of 20 μm, a pitch of 20 μm, and 13 in a 500 μm width region. Grooves are formed.

  In this embodiment, a plurality of reaction vessel air vent channels 21 are provided. A plurality of reaction vessel air vent channels 19 are connected to each reaction vessel air vent channel 21. The reaction container air vent channel 21 is for connecting the reaction container air vent channel 19 to an air drain space 31 formed in the base plate 3. The dimensions of the grooves constituting the reaction vessel air vent channel 21 are, for example, a depth of 400 μm and a width of 500 μm.

  The drain space air vent channel 23 is for connecting the liquid drain space 29 to a port of a rotary switching valve 87 described later. One end of the drain space air vent channel 23 is disposed on the liquid drain space 29. The other end of the drain space air vent channel 23 is connected to a channel 23 a formed of a through hole provided in the base plate 3. The flow path 23a is connected to a port of a rotary switching valve 87 described later. The dimensions of the grooves constituting the drain space air vent channel 23 are, for example, a depth of 400 μm and a width of 500 μm.

  The drain space air vent channel 25 is for connecting the air drain space 31 to a port of a rotary switching valve 87 described later. One end of the drain space air vent channel 25 is disposed on the air drain space 31. The other end of the drain space air vent channel 25 is connected to a channel 25 a formed of a through hole provided in the base plate 3. The flow path 25a is connected to a port of a rotary switching valve 87 described later. The dimensions of the grooves constituting the drain space air vent channel 25 are, for example, a depth of 400 μm and a width of 500 μm.

  A flow path cover 33 (not shown in FIG. 1A) is disposed on the flow path base 11. The flow path cover 33 is for fixing the flow path base 11 to the base plate 3. A through hole is formed in the flow path cover 33 at a position on the reaction vessel 5.

  Referring to FIGS. 1 and 4 to 6, the sample container storage unit 36, the reagent container storage unit 38, and the air suction unit are placed on the base plate 3 at a position different from the arrangement region of the reaction container 5 and the drain spaces 29 and 31. A container housing portion 40 is formed. A sample container 35 is accommodated in the sample container accommodating portion 36, a reagent container 37 is accommodated in the reagent container accommodating portion 38, and an air aspirating 39 is accommodated in the air aspirating container accommodating portion 40. The sample container 35, the reagent container 37, and the air suction container 39 constitute a sealed container for the reaction container plate of the present invention.

  As shown in FIG. 4, a sample channel 35 a penetrating from the bottom to the back surface of the sample container housing portion 36 and a sample container air vent flowing from the front surface to the back surface are formed on the base plate 3 in the vicinity of the sample container housing portion 36. A path 35b is formed. Three locking claws 35 c for holding the sample container 35 are arranged on the surface of the base plate 3 around the opening of the sample container housing portion 36.

  At the bottom of the sample container housing part 36, a projecting projection channel 35d is provided which projects toward the opening side of the sample container housing part 36. The base end side end of the projection channel 35d is connected to the sample channel 35a. The front end surface of the protruding channel 35d is inclined with respect to the protruding direction of the protruding channel 35d.

  On the surface of the base plate 3 in the vicinity of the sample container housing portion 36, a projecting second projecting channel 35e that projects upward is formed. The end portion on the proximal end side of the second protruding flow path 35e is connected to the sample container air vent flow path 35b. The tip surface of the second protrusion channel 35e is inclined with respect to the protruding direction of the second protrusion channel 35e.

  An annular packing 35f is provided on the outer peripheral side surface of the base end portion of the projection flow path 35d and the outer peripheral side surface of the base end portion of the second projection flow path 35e. For example, it is formed of an elastic material such as silicone rubber or PDMS.

  The sample container 35 disposed in the sample container housing portion 36 includes a sample container main space 35g, a sample container air vent channel 35h, and a sample container air vent space 35i.

  The sample container main space 35g is provided so as to penetrate from the upper surface to the lower surface of the sample container main body formed of a resin material such as polypropylene or polycarbonate. The opening on the lower surface side of the sample container main space 35g is sealed with a film 35j (penetrable portion) made of, for example, aluminum and attached to the lower surface of the sample container main body. The opening on the upper surface side of the sample container main space 35g is sealed with a film 35k made of, for example, aluminum attached to the upper surface of the sample container main body.

  The sample container air vent channel 35h is formed by covering a groove formed on the upper surface of the sample container body with a film 35k. The groove for forming the sample container air vent channel 35h is formed by, for example, one or a plurality of pores having a width of 5 to 200 μm and a depth of 5 to 200 μm. This is for keeping the liquid tightness of the sample container main space 35g in a state where there is no pressure difference in the container air vent space 35i.

  The sample container air vent space 35i is formed in an insertion portion provided on the upper side surface of the sample container main body, and is provided so as to penetrate from the upper surface to the lower surface of the insertion portion. The opening on the lower surface side of the sample container air vent space 35i is sealed with a film 35l (second penetrable portion) made of, for example, aluminum, which is attached to the lower surface of the insertion portion. The opening on the upper surface side of the sample container air vent space 35i is sealed with a film 35k.

  A septum 41, which is an elastic member, is formed on the film 35k. The septum 41 is made of, for example, an elastic material such as silicone rubber or PDMS. The septum 41 can be penetrated by a dispensing device having a sharp tip, and the through-hole can be closed by elasticity when the dispensing device is pulled out after penetration. A septum stopper 43 for fixing the septum 41 is disposed on the septum 41. The septum stopper 43 has an opening on the sample container 35. In this embodiment, the reagent 45 is stored in advance in the sample container main space 35g. In FIG. 4, the septum stopper 43 is fixed to the sample container main body by a locking claw provided on the septum stopper 43, but the number of locking claw provided on the septum stopper 43 is arbitrary. The septum stopper 43 may be fixed to the sample container main body by any method. For example, the septum stopper 43 may be fixed to the sample container main body by an adhesive.

  Locking grooves 35m and 35n are formed on the side surface of the sample container main body. The locking grooves 35m and 35n are for holding the sample container 35 in the sample container housing portion 36 at the first holding position or the second holding position by the locking claw 35c. The locking groove 35m is formed below the locking groove 35n, and is for holding the sample container 35 at the first holding position (see (E)). The locking groove 35n is for holding the sample container 35 at the second holding position (see (F)). The locking claw 35c and the locking grooves 35m and 35n constitute a sealed container holding mechanism for the reaction container plate of the present invention. However, the sealing container holding mechanism is not limited to the one constituted by the locking claw 35c and the locking grooves 35m and 35n, and the sample container 35 (sealing container) is held at the first holding position and the second holding position. Any configuration may be used as long as it can be held.

  In the first holding position, as shown in (E), the film 35j and the protruding flow path 35d are arranged to face each other, and the film 35l and the second protruding flow path 35e are arranged to face each other. By pushing the sample container 35 toward the base plate 3 from the state of the first holding position, the sample container 35 can be moved to the second holding position.

  At the second holding position, as shown in (F), the tip of the projection channel 35d penetrates the film 35j and is inserted into the sample container main space 35g, and the tip of the second projection channel 35e is the film 35l. And is inserted into the sample container air vent space 35i. In the state of the second holding position, the sample container main space 35g and the sample container channel 35a are connected via the projection channel 35d, and the sample container air vent space 35i and the sample container air vent channel 35b pass through the projection channel 35e. Connected through. At this time, the lower surface of the sample container main body of the sample container 35 is pressed against the packings 35f and 35f. Thereby, the sample container main space 35g and the sample container channel 35a, and the sample container air vent space 35i and the sample container air vent channel 35b are connected with high airtightness. Thereby, liquid leakage and air leakage can be prevented. However, the method for preventing liquid leakage and air leakage is not limited to the provision of the packings 35f and 35f, as long as the method can connect the sample container 35 and the flow paths 35a and 35b with airtightness. Any method may be used.

  If the sample container 35 is arranged at the first holding position, the reaction container plate can be stored in a state where the sample container 35 and the sample container flow path 35a are separated, so that the reagent 45 and the dilution 45 are diluted in the sample container main space 35g. Even if a liquid solid such as water or a powdered solid such as a reagent is enclosed and stored in advance, the liquid or solid does not enter the sample container channel 35a during storage.

  Furthermore, in the first holding position, the sample container main space 35g and the sample container air vent space 35i are sealed, so that liquid such as the reagent 45 and dilution water may be sealed in the sample container main space 35g in advance. The liquid can be prevented from evaporating.

Next, the reagent container 37 and the reagent container storage unit 38 will be described with reference to FIG.
The reagent container housing portion 38 has the same structure as the sample container housing portion 36 described with reference to FIG. That is, a reagent channel 37a, a reagent container air vent channel 37b, a locking claw 37c, a projection channel 37d, a second projection channel 37e, and packings 37f and 37f are provided.

  The reagent container 37 has the same structure as the sample container 35 described with reference to FIG. However, the reagent container 37 does not include the septum 41 as compared with the sample container 35, and includes a cover 47 instead of the septum stopper 43. That is, the reagent container 37 includes a reagent container main space 37g, a reagent container air vent channel 37h, a reagent container air vent space 37i, films 37j, 37k, and 37l, locking grooves 37m and 37n, and a cover 47. . The cover 47 is for preventing the film 37k affixed on the upper surface of the reagent container main body from being damaged. Dilution water 49 is accommodated in the reagent container main space 37g. In FIG. 5, the cover 47 is fixed to the reagent container main body by a locking claw provided on the cover 47, but the number of the locking claw provided on the cover 47 is arbitrary. Further, any method may be used for fixing the cover 47 to the reagent container main body. For example, the cover 47 may be fixed to the reagent container main body with an adhesive.

  Similarly to the sample container 35, the reagent container 37 is also arranged in the reagent container housing portion 38 at the first holding position (see (E)) and the second holding position (see (F)). The connection between the reagent container 37 and the reagent container channel 37a and the reagent container air vent channel 37b is the same as the connection between the sample container 35, the sample container channel 35a and the reagent container air vent channel 35b described with reference to FIG. It is.

  If the reagent container 37 is arranged at the first holding position, the reaction container plate can be stored in a state where the reagent container 37 and the reagent container flow path 37a are separated, so that the reagent or dilution water is stored in the reagent container main space 37g. Even if a liquid such as 49 or a powdery solid such as a reagent is enclosed and stored in advance, the liquid or solid does not enter the reagent container channel 37a during storage.

  Furthermore, in the first holding position, the reagent container main space 37g and the reagent container air vent space 37i are sealed, so that a liquid such as a reagent or dilution water 49 may be sealed in the reagent container main space 37g in advance. The liquid can be prevented from evaporating.

Next, the air suction container 39 and the air suction container housing 40 will be described with reference to FIG.
The air suction container container 40 has the same structure as the reagent container container 38 described with reference to FIG. That is, an air suction channel 39a, an air suction container air vent channel 39b, a locking claw 39c, a projection channel 39d, a second projection channel 39e, and packings 39f and 39f are provided.

  The air suction container 39 has the same structure as the reagent container 37 described with reference to FIG. That is, the air suction container 39 includes an air suction container main space 39g, an air suction container air vent channel 39h, an air suction container air vent space 39i, films 39j, 39k, 39l, and locking grooves 39m, 39n. , And a cover 47. Liquid and solid are not accommodated in the air suction container main space 39g and are filled with air.

  Similarly to the sample container 35 and the reagent container 37, the air suction container 39 is also arranged in the air suction container housing portion 40 at the first holding position (see (E)) and the second holding position (see (F)). The The connection between the air suction container 39, the air suction container channel 39a, and the air suction container air vent channel 39b is the same as that of the sample container 35, the sample container channel 35a, and the reagent container air vent flow described with reference to FIG. This is the same as the connection of the path 35b.

  The description will be continued with reference to FIGS. 1 and 2. The reaction container plate 1 is provided with a syringe inside, and the syringe and each flow path provided in the reaction container plate 1 are selectively connected. A rotary type switching valve 87 that can be used is mounted. The rotary switching valve 87 is mounted at a position different from the arrangement region of the reaction vessel 5, the drain spaces 29 and 31, and the container housing portions 36, 38, and 40 so as to be rotatable in the in-plane direction of the base plate 3.

  The rotary switching valve 87 includes a syringe part and a flange part. The syringe part is positioned above and at the center of rotation of the rotary switching valve 87, and includes a cylinder 87a having an open upper end surface, a plunger 87c accommodated in the cylinder 87a, and a cover body 87d to form a syringe. The plunger 87c is supported by a cover body 87d so that it can slide in the cylinder 87a in a direction perpendicular to the rotational direction of the rotary switching valve 87. The flange part has a larger diameter than the syringe part and is provided at the lower end part of the syringe part. The syringe part and the flange part are integrated. The “syringe part” corresponds to the “insertion part” in the claims, and the “flange part” corresponds to the “non-insertion part” in the claims. In FIG. 1B, illustration of the plunger 87c, the cover body 87d, and the syringe air vent channel 87h provided in the cylinder 87a is omitted, and in FIG. 2A, the rotary switching valve 87 is easily seen. For convenience, the sample container 35, the sample container channel 35a, and the sample container air vent channel 35b are not shown. Details of the syringe will be described later.

  A flow path drawing plate 55 is attached to the lower surface of the base plate 3 at a position different from the arrangement region of the reaction vessels 5 with a seal plate 54 interposed therebetween. The base plate 3 and the flow path drawing plate 55 are provided with through holes 92 and 93 having an inner diameter substantially the same as that of the syringe portion of the rotary switching valve 87. The syringe portion of the rotary switching valve 87 is inserted into the through holes 92 and 93 from the lower surface side of the flow path drawing plate 55, and the tip portion protrudes from the upper surface side of the base plate 3. The distal end portion of the syringe portion that protrudes toward the upper surface side of the base plate 3 is called a “projection portion” in the claims. A syringe channel 87 b connected to the syringe is provided inside the flange portion of the rotary switching valve 87. A syringe port 87j connected to the syringe flow path 87 and an arcuate flow path port 87i connected to an air vent flow path 87h described later are provided on the upper surface of the flange portion. The upper surface of the flange portion constitutes the syringe port arrangement portion in the claims. The seal plate 54 is made of an elastic material such as silicone resin. The seal plate 54 is not shown in the drawings after FIG.

  A plurality of flow path ports connected to the flow paths 13a, 23a, 25a, 35a, 35b, 37a, 37b, 39a, 39b are arranged in a region facing the upper surface of the flange portion on the lower surface of the flow path drawing plate 55. The flow path drawing plate 55 includes a drawing flow path for connecting the flow paths 13a, 23a, and 25a of the base plate 3 to the flow path ports, and part or all of 35a, 35b, 37a, 37b, 39a, and 39b. Grooves or through-holes are formed to be configured and connected to the respective flow path ports, and further, an air vent flow path 53b of a bellows 53 described later is formed. Portions of these flow channels facing the upper surface of the flow channel extraction plate 55 are surrounded by a protruding line portion 55c protruding from the surface of the flow channel extraction plate 55, and the edges are raised. Thereby, the surface pressure between the upper surface of the flow channel drawing plate 55 and the lower surface of the seal plate 54 is concentrated on the edge portions of these flow channels, and the convex portions 55c are provided for the sealing performance of these flow channels. Higher than if not. Although not shown, the edges of the flow paths 13a, 23a, 25a, 35a, 35b, 37a, 37b, 39a, 39b facing the lower surface of the base plate 3 may also rise. Then, the surface pressure between the base plate 3 and the seal plate is concentrated on the edges of the flow paths, and the liquid tightness can be further improved.

In addition, the area | region where the flow path port of the flow path drawer plate 55 lower surface is provided comprises the flow path port arrangement | positioning part in a claim.
With the above configuration, the rotary switching valve 87 can be rotated to connect the arcuate flow path port 87 i or the syringe port 87 j to any flow path port on the lower surface of the flow path drawing plate 55.

  A seal plate 88 is sandwiched between the lower surface of the flow channel drawer plate 55 and the upper surface of the rotary type switching valve 87, and the flow port port arrangement portion on the lower surface of the flow channel extraction plate 55 and the syringe port arrangement portion on the upper surface of the rotary type switching valve 87. The sealing performance between the two has been improved. The seal plate 88 is fixed to the flow path drawing plate 55 side so as not to rotate together with the rotary type switching valve 87. The sealing plate 88 is made of a material such as silicone resin, for example, having a certain elasticity and a contact surface with the rotating rotary switching valve 87 having a certain sliding property.

  A bellows 53 is provided at a position different from the arrangement region of the reaction vessel 5 of the base plate 3, the drain spaces 29 and 31, and the vessels 35, 37 and 39. The bellows 53 has an internal space sealed, and the internal capacity is passively variable by expanding and contracting. For example, the bellows 53 is disposed in a through hole 53 a provided in the base plate 3. The channel drawing plate 55 includes an air vent channel 53 b that communicates with the bellows 53. The air vent channel 53 b is connected to a channel port on the lower surface of the channel drawer plate 55 that can be connected to the arc-shaped channel port 87 i of the rotary switching valve 87.

  A fixing member engaging portion 55a that protrudes vertically downward so as to surround the flange portion of the rotary switching valve 87 is provided on the lower surface of the flow path drawing plate 55. The fixed member engaging portion 55a holds the rotary switching valve 87 rotatably inside thereof, and has an engagement hole 55b that engages with the claw portion 91a of the backup ring 91, and is provided from the lower surface side of the rotary switching valve 87. The backup ring 91 inserted is held. The backup ring 91 is a ring-shaped member having substantially the same diameter as the outer diameter of the flange portion of the rotary switching valve 87, and the fixing member engaging portions 55a are engaged at a plurality of equal positions, for example, four locations on the outer peripheral surface thereof. A claw portion 91a that engages with the hole 55b is provided. The lower peripheral edge of the flange portion of the rotary switching valve 87 is recessed for fitting the backup ring 91, the backup ring 91 is fitted into the recess, and the claw portion 91a is an engagement hole of the fixing member engaging portion 55a. The backup ring 91 is fixed by engaging with 55b. At this time, the upper surface 91 b of the backup ring 91 fixed to the fixing member engaging portion 55 a comes into contact with the bottom surface of the recess on the lower surface side peripheral portion of the rotary switching valve 87 to support and fix the rotary switching valve 87. The depth of the recess at the base end portion of the rotary switching valve 87, the position of the claw portion 91a of the backup ring 91, and the position of the engagement hole 55b of the fixing member engaging portion 55a are fixed to the fixing member engaging portion 55a. Further, the upper surface 91b of the backup ring 91 is set so as to further press the bottom surface of the recess on the lower surface side of the rotary type switching valve 87 toward the channel drawing plate 55 side. As a result, the peripheral edge of the upper surface of the flange portion of the rotary switching valve 87 is pressed against the flow channel drawing plate 55 side, and the arc-shaped flow port 87i and syringe port 87j provided on the upper surface of the flange portion are connected to each flow port. The sealability of the part is improved.

  The engagement hole 55a and the claw portion 91a constitute a “first engagement structure portion” in the claims, and the engagement hole 55a is a structure on the fixing member engagement portion 55 side of the first engagement structure portion. The claw portion 91a constitutes a structure on the backup ring 91 side of the first engagement structure portion. The outer surface of the claw portion 91 a is inclined so as to converge toward the upper surface 91 b of the backup ring 91. Thus, when the backup ring 91 is fitted into the lower surface side of the rotary type switching valve 87, when the backup ring 91 is pushed inward, the fixing member engaging portion 55a is integrally formed with the flow path drawer plate 55 and the resin. Since it is formed, it elastically deforms and spreads along the inclination of the outer surface of the claw portion 91a, and when the claw portion 91a reaches the position of the engagement hole 55b, it returns to its original state, thereby engaging with the claw portion 91a. The mating hole 55b is engaged. Accordingly, the claw portion 91a and the engagement hole 55b are automatically engaged only by pushing the backup ring 91 to a predetermined position, so that the backup ring 91 can be easily attached. In addition, the structure of this 1st engagement structure part is an example. In the first engagement structure portion, at least one of the inner surface of the structure on the fixing member engagement portion 55a side or the outer surface of the structure on the backup ring 91 side is on the upper surface 91b side of the backup ring 91 (on the channel drawing plate 55 side). It only needs to be tilted in the direction of closing.

  Next, the rotor cap 89 is fitted and attached to the distal end side of the syringe portion of the rotary switching valve 87 protruding from the upper surface of the base plate 3. The rotor cap 89 is formed by integral molding with resin. As shown in FIG. 2B, the rotor cap 89 includes a through hole 89a at the center of the upper surface and a claw portion 89b protruding inward from the periphery. The through hole 89a is for guiding a mechanism for driving the plunger 87c from the upper end surface of the syringe part into the cylinder 87a. A hooking structure comprising a convex portion 87e and a concave portion 87f is provided on the peripheral surface of the syringe portion of the rotary switching valve 87, and at the same time the claw portion 89b of the rotor cap 89 enters the concave portion 87f and engages with the convex portion 87e. When the lower surface of the rotor cap 89 is in contact with the upper surface of the base plate 3, the rotary switching valve 87 is prevented from falling off from the through holes 92 and 93. The positions and dimensions of the convex portions 87e and concave portions 87f on the peripheral surface of the syringe portion of the rotary switching valve 87 and the claw portion 89b of the rotor cap 87 are such that the upper surface of the flange portion of the rotary switching valve 87 is pulled upward by the rotor cap 89. Is set to As a result, the center portion of the upper surface of the flange portion of the rotary switching valve 87 is pulled upward and pressed against the flow channel drawing plate 55 side, and the syringe port 87j and the arc-shaped flow port 87i on the upper surface of the flange portion and the flow channel drawing plate. The sealability of connection with each flow path port on the lower surface of 55 is improved.

  The convex portion 87e, the concave portion 87f, and the claw portion 89b constitute the “second engagement structure portion” in the claims. In this embodiment, in particular, the convex portion 87e constitutes a structure on the syringe portion side of the second engagement structure portion, and the claw portion 89b constitutes a structure on the rotor cap 87 side of the second engagement structure portion. The outer surface of the convex portion 87e is inclined so as to converge toward the distal end side of the syringe portion, and the inner surface of the claw portion 89b is inclined so as to converge toward the lower surface side of the rotor cap 87. Thereby, when the rotor cap 87 is fitted from the tip of the syringe part, the inner surface of the claw part 89b elastically spreads the rotor cap 87 made of resin along the outer surface of the convex part 87e, and the claw part 89b When the position of the concave portion 89b is reached, the inner diameter of the rotor cap 89 returns to the original state, the claw portion 89b is inserted into the concave portion 87f, and the convex portion 87e and the claw portion 87e are engaged. Therefore, the convex portion 87e and the claw portion 87e are automatically engaged simply by fitting the rotor cap 87 to the tip of the syringe portion and pushing it to a predetermined position, so that the rotor cap 87 can be easily attached. In addition, the structure of this 2nd engagement structure part is an example. The second engagement structure portion may be inclined in a direction in which at least one of the inner surface of the structure on the rotor cap 87 side or the outer surface of the structure on the syringe portion side closes toward the tip of the syringe portion.

  The rotary switching valve 87 can be fixed to the main body of the reaction vessel plate 1 with only one of the rotor cap 89 and the backup ring 91. However, most of the rotary switching valve 87 is formed by integral molding with resin for the purpose of reducing the number of parts and reducing the cost. Therefore, when only one of the central part and the peripheral part of the flange part is pressed against the flow path drawing plate 55 side, the force that presses the upper surface of the flange part toward the flow path drawing plate 55 side due to elastic deformation of the flange part is uneven. Therefore, there is a risk of a seal failure. If both the rotor cap 89 and the backup ring 91 are attached, the upper surface of the flange portion of the rotary switching valve 87 can be pressed against the flow path extraction plate 55 side at both the central portion and the peripheral portion. The pressing force toward the road drawer plate 55 becomes uniform, and high sealing performance can be obtained.

  As will be described later, in this embodiment, the rotor cap 89 also plays a role of sandwiching and supporting the upper end portion of the cover body 87d constituting the syringe with the upper end surface of the syringe portion of the rotary switching valve 87. The front end side fixing member that simultaneously engages the syringe portion of the rotary switching valve 87 and the upper surface of the base plate 3 to fix the rotary switching valve 87 does not need to be a cap-like member such as the rotor cap 89. It may be a ring-shaped member.

  By the way, the syringe in the rotary switching valve 87 includes the cylinder 87a, the plunger 87c, and the cover body 87d as described above. One end of the cover body 87d is connected to the upper end of the cylinder 87a, the other end is connected to the plunger 87c, and the cover body 87d is flexible in the sliding direction of the plunger 87c. The cover body 87d is for blocking the portion of the inner wall of the cylinder 87a that the plunger 87c contacts with the atmosphere outside the cylinder 87a while maintaining airtightness. Accordingly, the space 87g surrounded by the cylinder 87a, the plunger 87c, and the cover body 87d is sealed. One end of the cover body 87d is fixed by a rotor cap 89, and the other end is bonded to the upper surface of the plunger 87c with an adhesive while ensuring airtightness. However, the method and position for connecting the cover body 87d to the cylinder 87a and the plunger 87c are not limited to this.

  As described above, the cover body 87d is connected to the cylinder 87a and the plunger 87c to form a sealed space 87g surrounded by the cylinder 87a, the plunger 87c, and the cover body 87d, so that the cover body 87d is interposed between the cylinder 87a and the plunger 87c. In this way, it is possible to prevent foreign matter from entering from the outside and environmental contamination of the liquid to the outside. Since the cover body 87d has flexibility in the sliding direction of the plunger 87c, the sliding operation of the plunger 87c is possible.

  Inside the rotary switching valve 87, there is also provided a syringe air vent channel 87h having one end connected to the sealed space 87g and the other end connected to the arcuate channel port 87i on the upper surface of the flange portion. By providing the syringe air vent channel 87h, the sealed space 87g is made independent from the outside, and the pressure change in the sealed space 87g due to the change in the internal capacity of the sealed space 87g when the plunger 87c slides is reduced. The plunger 87c can be smoothly slid by being relaxed. In FIG. 1A, illustration of the syringe air vent channel 87h is omitted.

  In this embodiment, the plunger 87c and the cover body 87d are formed by separate members, but the plunger and the cover body may be integrally formed. An example of the integrally formed plunger and cover material is silicone rubber.

  The rotation of the rotary switching valve 87 causes the cylinder 87a to be connected to one of the flow paths 13a, 35a, 37a, 39a via the syringe flow path 87b and the syringe port 87j, and at the same time, the air vent flow path 53b flows. Connected to at least one of the paths 23a, 25a, 35b, 37b, 39b.

  In the reaction vessel plate 1, the injection channel 17 is formed so as to maintain the liquid tightness of the reaction vessel 5 with no pressure difference between the reaction vessel 5 and the injection channel 17. The reaction vessel air vent channel 19 is also formed so as to maintain the liquid tightness of the reaction vessel 5 in the state where there is no pressure difference between the reaction vessel 5 and the reaction vessel air vent channel 19. The main flow path 13 of the reaction vessel flow path, the liquid drain space 29 to which the main flow path 13 is connected, and the drain space air vent flow path 23 can be sealed by rotation of the rotary switching valve 87. The containers 35, 37, and 39 are sealed with a septum 41 or a film 47. The flow paths 35 a, 35 b, 37 a, 37 b, 39 a, 39 b connected to the containers 35, 37, 39 can be sealed by switching the rotary type switching valve 87. One end of the air vent channel 53b is connected to the bellows 53 and sealed. Thus, the container and flow path inside the reaction container plate 1 are formed in a closed system. Even when the air vent channel 53b is connected to the atmosphere outside the reaction vessel plate 1 without the bellows 53, the air vent channel 53b is changed by switching the rotary switching valve 87. Since it can block | block from the flow paths other than the container inside the reaction container plate 1 and the air vent flow path 53b, the container and flow path in which a liquid is accommodated or a liquid flows can be made into a closed system.

FIG. 7 is a cross-sectional view showing a reaction processing apparatus for processing the reaction container plate 1 shown in FIG. Since the structure of the reaction vessel plate 1 is the same as that shown in FIG.
The reaction processing apparatus includes a temperature adjustment mechanism 67 for adjusting the temperature of the reaction vessel 5, a syringe drive unit 69 for driving the syringe, and a switching valve drive unit 71 for switching the rotary switching valve 87. .

  8 to 14 are plan views for explaining the operation of introducing the sample liquid from the sample container 35 into the reaction container 5. This operation will be described with reference to FIGS. 1, 2 and 8 to 14.

  In a state where the sample container 35, the reagent container 37, and the air suction container 39 are held at the first holding position, a dispensing device having a sharp point (not shown) is used to penetrate the septum 41 on the sample container 35, for example. Dispense 5 μL of the sample solution into the sample container 35. After dispensing the sample solution, pull out the dispensing device. The through hole of the septum 41 when the dispensing instrument is pulled out is closed by the elasticity of the septum 41.

  The sample container 35, the reagent container 37, and the air suction container 39 held at the first holding position are pushed into the base plate 3 side and moved to the second holding position (see FIGS. 4 to 6).

The syringe drive unit 69 is connected to the plunger 87 c of the syringe, and the switching valve drive unit 71 is connected to the rotary switching valve 87.
As shown in FIG. 8, the rotary switching valve 87 is rotated from the state of FIG. 1A to connect the sample channel 35a and the syringe port 87j, and the sample container air vent channel 35b is changed to the air vent channel 53b. Connecting. At this time, the air vent channels 37b and 39b are also connected to the air vent channel 53b. For example, 45 μL of the reagent 45 is accommodated in the sample container 35.

  The plunger 87c of the syringe is slid to mix the sample liquid and the reagent 45 in the sample container 35. Thereafter, the mixed liquid in the sample container 35 is sucked, for example, by 10 μL. At this time, since the sample container 35 is connected to the bellows 53 via the air vent channel 35b, the arc-shaped channel port 87i, and the air vent channel 53b, the bellows is changed with the change in the gas capacity in the sample container 35. 53 expands and contracts. Further, the cover 87d is deformed by the sliding of the plunger 87c, and the internal capacity of the sealed space 87g (see FIG. 1C) is changed. Since the sealed space 87g is connected to the bellows 53 via the syringe air vent channel 87h, the arc-shaped channel port 87≡ and the air vent channel 53b, the bellows 53 expands and contracts even when the internal capacity of the sealed space 87g changes. To do. Also in the operation process described below, the bellows 53 expands and contracts with the change in the internal capacity of the sealed space 87g due to the sliding of the plunger 87c.

  As shown in FIG. 9, the rotary switching valve 87 is rotated to connect the reagent channel 37a and the syringe channel 87b, and the reagent container air vent channel 37b is connected to the air vent channel 53b. For example, 190 μL of dilution water 49 is accommodated in the reagent container 37. The mixed liquid sucked into the syringe in the step of FIG. 8 is injected into the reagent container 37, and the plunger 87c is reciprocated up and down to mix with the mixed liquid and the dilution water 49. For example, 200 μL of the diluted mixture is sucked with a syringe. At this time, since the reagent container 37 is connected to the bellows 53 via the air vent channel 37b, the arc-shaped channel port 87i, and the air vent channel 53b, the bellows is changed with the change in the gas capacity in the reagent container 37. 53 expands and contracts.

  As shown in FIG. 10, the rotary switching valve 87 is rotated to connect the syringe port 87j to the flow path port connected to the one end 13a of the main flow path 13, and the liquid drain space 29 and the air drain space 31 are connected to the arc-shaped flow path. The bellows 53 is connected via the port 87i. By driving the plunger 87c in the pushing direction, the diluted mixed solution sucked using the syringe in the step of FIG. The diluted mixed liquid injected from the flow path 13a side into the main flow path 13 fills the metering flow path 15 in order from the flow path 13a side and reaches the liquid drain space 29 as indicated by the embossments and arrows. In the introduction pressure state when the diluted mixture is introduced into the main channel 13 and the metering channel 15, the injection channel 17 allows gas to pass but does not allow the diluted mixture to pass. The gas in the metering channel 15 moves into the reaction vessel 5 through the injection channel 17 as the metering channel 15 is filled with the diluted mixed solution. As this gas moves, a part of the gas in the reaction vessel 5 moves to the reaction vessel air vent channels 19 and 21. Furthermore, the gas in the flow path from the reaction container air vent flow path 19 to the bellows 53 sequentially moves to the bellows 53 side (see white arrow). Further, when the diluted mixed liquid is injected into the liquid drain space 29, the gas in the flow path from the liquid drain space 29 to the bellows 53 sequentially moves toward the bellows 53 (see the white arrow). As a result, the bellows 53 expands.

  As shown in FIG. 11, the rotary switching valve 87 is rotated to connect the syringe port 87j to the flow path port connected to the air suction flow path 39a, and the air suction container air vent flow path 39b is connected to the arc-shaped flow path port. Connected to bellows 53 through 87i. By driving the plunger 87c to the suction side, the gas in the air suction container 39 is sucked. At this time, the bellows 53 contracts as the pressure in the air suction container 39 is reduced (see white arrow).

  As shown in FIG. 12, the rotary switching valve 87 is rotated to connect the syringe port 87j to the same state as in FIG. 10, that is, the flow path port connected to the one end 13a of the main flow path 13, and the liquid drain space 29, air drain The space 31 is connected to the bellows 53 via the arc-shaped flow path port 87i. By driving the plunger 87c in the pushing direction, the gas sucked in the step of FIG. 11 is sent to the main channel 13 to purge the diluted mixed solution in the main channel 13 (see the white arrow). In the purge pressure state at this time, the dilute mixed liquid does not pass through the injection flow path 17, and therefore the dilute mixed liquid remains in the measuring flow path 15 (see embossing). The purged diluted liquid mixture is accommodated in the liquid drain space 29. Further, when the diluted liquid mixture is injected into the liquid drain space 29, the gas in the flow path from the liquid drain space 29 to the bellows 53 sequentially moves toward the bellows 53, and the bellows 53 expands (see the white arrow). ).

  As shown in FIG. 13, the rotary switching valve 87 is rotated to connect the syringe port 87j to the port connected to the air suction flow path 39a in the same state as in FIG. 39b is connected to the bellows 53 via the arc-shaped channel port 87i. By driving the plunger 87c to the suction side, the gas in the air suction container 39 is sucked.

  As shown in FIG. 14, the rotary switching valve 87 is rotated to connect the syringe port 87j to the port connected to the one end 13a of the main flow path 13, and the air drain space 31 is connected to the bellows 53 via the arc-shaped flow path port 87i. However, unlike the case of FIGS. 10 and 12, the liquid drain space 29 is not connected to the arcuate flow path port 87i. In this state, the plunger 87c is driven in the pushing direction. Since the downstream end of the main flow path 13 is not connected to the bellows 53, the inside of the main flow path 13 is pressurized larger than the liquid introduction pressure and the purge introduction pressure. As a result, the diluted mixed solution in the metering channel 15 is injected into the reaction vessel 5 through the injection channel 17. After the diluted mixed solution is injected into the reaction vessel 5, a part of the gas in the main channel 13 flows into the reaction vessel 5 through the metering channel 15 and the injection channel 17. At this time, the gas between the reaction vessel 5 and the bellows 53 sequentially moves toward the bellows 53, and the bellows 53 expands (see the white arrow).

After the rotary switching valve 87 is connected as shown in FIG. 1 and the container, flow path and drain space inside the reaction container plate 1 are sealed, the reaction container 5 is heated by the temperature control mechanism 67 to melt the wax 9. Thereby, the diluted mixed solution injected into the reaction vessel 5 enters under the wax 9, and the diluted mixed solution and the reagent 7 are mixed and reacted. Thus, according to the reaction container plate 1, the reaction process can be performed in a closed system.
Before injecting the diluted mixed solution into the reaction vessel 5, the reaction vessel 5 is heated by the temperature control mechanism 67 to melt the wax 9, and the wax 9 is injected when the diluted mixed solution is injected into the reaction vessel 5. May be melted. In this case, the diluted mixed solution injected into the reaction vessel 5 immediately enters under the wax 9, and the diluted mixed solution and the reagent 7 are mixed and reacted. Even if the connection state of the rotary switching valve 87 is the state shown in FIG. 14, the sealed system is secured by the bellows 53. If the rotary switching valve 87 is brought into the connected state shown in FIG. 1 after the diluted mixed solution is injected, the container, flow path and drain space inside the reaction vessel plate 1 can be sealed. Here, the timing of switching the rotary type switching valve 87 to the connected state of FIG. 1 may be any timing from immediately after the injection of the diluted mixed solution to the end of the reaction of the diluted mixed solution and the reagent 7, It may be after the reaction of the reagent 7 is completed.
Thus, according to the reaction vessel plate 1, the reaction process can be performed in a closed system, and a closed system can be formed before and after the reaction process.

  In this embodiment, the grooves for forming the flow paths 13, 15, 17, 19, 21, and 23 are formed in the flow path base 11, but the present invention is not limited to this, and the flow of these is not limited. A groove for forming all or part of the path may be formed on the surface of the base plate 3.

  FIG. 15 is an enlarged schematic cross-sectional view showing the vicinity of a reaction vessel in another embodiment of the reaction vessel plate. This embodiment is the same as the above-described embodiment described with reference to FIGS. 1 to 14 except that a flow path spacer is disposed between the base plate and the flow path base.

  A flow path spacer 73 is disposed on the base plate 3 so as to cover the arrangement region of the reaction vessels 5, and a flow path base 11 and a flow path cover 33 are further disposed in that order. The channel spacer 73 is made of, for example, PDMS or silicone rubber. The thickness of the channel spacer 73 is, for example, 0.5 to 5.0 mm. The flow path spacer 73 includes a convex portion 75 protruding into the reaction container 5 for each reaction container 5. The convex portion 75 has a substantially trapezoidal cross section. For example, the base end has a width of 1.0 to 2.8 mm, the tip has a width of 0.2 to 0.5 mm, and the tip has a base end. It is thinner than Further, the surface of the convex portion 75 is subjected to super water repellent treatment. However, the surface of the convex portion 75 may not necessarily be subjected to the water repellent treatment.

Further, the flow path spacer 73 is provided with an injection flow path 77 formed of a through hole penetrating from the tip end portion of the convex portion 75 to the opposite surface for each position where the convex portion 75 is formed. The inner diameter of the injection channel 77 is, for example, 500 μm. The opening on the flow channel base 11 side of the injection flow channel 77 is connected to the injection flow channel 17 of the flow channel base 11. In this embodiment, the channel base 11 is not provided with the recess 27 as compared with the above-described embodiment described with reference to FIGS.
Further, the flow path spacer 73 is also provided with a reaction container air vent flow path 79 including a through hole for communicating the reaction container air vent flow path 19 of the flow path base 11 and the reaction container 5.

  Although not shown, the channel spacer 73 includes both end portions of the main channel 13, end portions of the reaction vessel air vent channel 21 on the air drain space 31 side, and both ends of the drain space air vent channels 23 and 25. The part is provided with a through hole, and the flow paths 13, 21, 23, 25 are connected to the containers 29, 31 or the flow paths 23a, 25b provided in the base plate 3.

  In this embodiment, the end of the injection flow channel 77 opposite to the injection flow channel 15 (the other end of the injection flow channel) is disposed at the tip of a convex portion 75 formed to protrude from the inner upper surface of the reaction vessel 5. Therefore, the liquid injected into the reaction vessel 5 through the injection flow channels 15 and 77 can be easily dropped into the reaction vessel 5.

  Further, when the liquid is discharged from the tip of the convex portion 75 through the injection channel 77, the tip of the convex portion 75 is adjusted so that the liquid droplet formed at the tip of the convex portion 75 contacts the side wall of the reaction vessel 5. If it arrange | positions in the side wall vicinity of the reaction container 5, a liquid can be inject | poured in the reaction container 5 along the side wall of the reaction container 5, and a liquid can be inject | poured in the reaction container 5 more reliably. However, the formation position of the convex portion 75 may be a position where a droplet formed at the tip of the convex portion 75 does not contact the side wall of the reaction vessel 5.

FIG. 16 is a schematic cross-sectional view showing, in an enlarged manner, the vicinity of the reaction vessel of another embodiment of the reaction vessel plate.
Compared with the embodiment described with reference to FIG. 15, this embodiment further includes an insertion portion 81 inside the reaction vessel 5. The distal end of the insertion portion 81 is disposed below the distal end of the convex portion 75. Thereby, it becomes easy to guide the droplet formed at the tip of the convex portion 75 into the reaction vessel 5. In particular, it is particularly effective if a hydrophilic treatment is applied to at least the tip surface of the insertion portion 81.

FIG. 17 is an enlarged schematic cross-sectional view showing the vicinity of a reaction vessel of still another embodiment of the reaction vessel plate.
In this embodiment, compared to the embodiment described with reference to FIG. 16, the step portion 83 formed on the side wall of the reaction vessel 5 and the upper surface of the reaction vessel 5 are formed on the upper surface of the step portion 83 with a gap. Further provided is a convex ridge 85. The step part 83 and the protruding line part 85 are formed in an annular shape when viewed from above. The tip of the ridge 85 is disposed at a distance from the side wall of the reaction vessel 5.
Since the tip of the ridge 85 is spaced from the upper surface and side surface of the reaction vessel 5, the liquid contained in the reaction vessel 5 reaches the upper surface of the reaction vessel 5 along the side wall of the reaction vessel 5. Can be prevented. This effect is particularly effective when a water-repellent treatment is performed on at least the tip of the ridge 85.

The structure provided with the step part 83 and the protruding line part 85 shown in FIG. 17 can also be applied to the embodiment shown in FIG.
Moreover, in each Example demonstrated with reference to FIG.15, FIG16 or FIG.17, the groove | channel for forming the flow path 13,15,17,19,21,23 is formed in the flow path base 11. FIG. However, the present invention is not limited to this, and the grooves for forming all or part of the flow paths are the surface of the flow path spacer 73 on the flow path base 11 side, the flow path spacer 73 on the base plate 3 side. It may be formed on either the surface or the base plate 3 surface.

  The embodiments of the present invention have been described above, but the present invention is not limited to these, and the shape, material, arrangement, number, dimensions, flow path configuration, etc. are examples, and are described in the claims. Various modifications are possible within the scope of the present invention.

For example, the bellows 53 connected to the air vent channel 53b may have another structure as long as the capacity is a variable capacity member whose internal capacity is passively variable. Examples of such a structure include a bag-like material made of a flexible material and a syringe-like material.
Further, the capacity variable member such as the bellows 53 may not necessarily be provided.
In addition, if the containers 35, 37, and 39 do not contain a liquid such as a reagent in advance, it is not always necessary to provide the flow paths 35e, 37e, and 39e made of pores in a part of the air vent flow path.

Moreover, in the said Example, although the base plate 3 is formed with one component, the base plate may be formed with several components.
The reagent in the reaction vessel 5 may be a dry reagent.
In addition, the reagent may not be stored in advance in the sample container 35 or the reaction container 5.
In the above embodiment, the dilution water 49 is stored in the reagent container 37, but the reagent may be stored instead of the dilution water 49.

  Further, the base plate 3 may be provided with a gene amplification container for performing a gene amplification reaction. For example, if the reagent container 37 is emptied, it can be used as a gene amplification container.

In addition, if a reagent for performing a gene amplification reaction is accommodated in the reaction container 5, the gene amplification reaction can be performed in the reaction container 5.
Further, when a gene is contained in the liquid introduced into the main flow path 13, a probe that reacts with the gene may be provided in the reaction vessel 5.

  In the above embodiment, when the liquid filled in the metering channel 15 is injected into the reaction vessel 5 through the injection channel 17, the inside of the main channel 13 after air purge is pressurized to supply the liquid to the reaction vessel 5. However, the reaction treatment method of the present invention is not limited to this. For example, the flow channel configuration is changed so that the inside of the reaction vessel air vent channel 21 can be set to a negative pressure using a syringe, and measurement is performed by setting the inside of the reaction vessel air vent channel 21 and thus the reaction vessel 5 to a negative pressure. The liquid filled in the flow path 15 may be injected into the reaction vessel 5 through the injection flow path 17. Alternatively, a separate syringe may be prepared so that the liquid is injected into the reaction vessel 5 with a positive pressure in the main channel 13 and a negative pressure in the reaction vessel 5.

  In the above embodiment, the penetrable portion and the second penetrable portion of the sealed container are formed by, for example, films 35j, 35l, 37j, 37l, 39j, and 39l made of aluminum. The 2 penetrable portion is not limited to this, and may be a film of another material or may be formed of the same material as the container body. For example, when the container body is formed of a resin material such as polypropylene or polycarbonate, the thickness of the resin material in the penetrable portion and the second penetrable portion is set to a thickness that can penetrate through the protruding flow channel and the second protruding flow channel. Then, the penetrable part and the second penetrable part can be formed by integral molding with the container body. Here, the thicknesses of the penetrable part and the second penetrable part when the penetrable part and the second penetrable part are formed integrally with the container body are, for example, 0.01 to 0.5 mm.

  Moreover, in the said Example, although the one main flow path 13 was provided and all the measurement flow paths 15 were connected to the main flow path 13, a flow path structure is not limited to this. For example, a plurality of main channels may be provided, and one or a plurality of metering channels may be connected to each main channel.

  The present invention can be used for measurement of various chemical reactions and biochemical reactions.

It is a figure which shows one Example of reaction container plate, (A) is a schematic top view, (B) is a cross section in the AA position of (A), a bellows, drain space, a measurement flow path, an injection flow path FIG. 4 is a schematic cross-sectional view including a cross section of a sample container air vent channel, and (C) is a cross-sectional view of a channel drawer plate. (A) is sectional drawing which shows the rotary type switching valve 87 of the reaction container plate of the Example, (B) is a perspective view of the rotor cap 89, (C) is a perspective view of the backup ring 91. FIG. It is the schematic which shows the one reaction container vicinity of the Example, (A) is a top view, (B) is a perspective view, (C) is sectional drawing. It is the figure which expanded and showed the sample container accommodating part and sample container of the Example, (A) is a top view of a sample container accommodating part, (B) is sectional drawing in the BB position of (A), (C) is a plan view of the sample container, (D) is a cross-sectional view at the C-C position in (C), (E) is a cross-sectional view in which the sample container is arranged in the sample container accommodating portion at the first holding position, (F) ) Is a cross-sectional view in which the sample container is disposed in the sample container housing portion at the second holding position. It is the figure which expanded and showed the reagent container accommodating part and reagent container of the Example, (A) is a top view of a reagent container accommodating part, (B) is sectional drawing in the DD position of (A), (C) is a plan view of the reagent container, (D) is a cross-sectional view at the EE position of (C), (E) is a cross-sectional view in which the reagent container is arranged in the reagent container housing portion at the first holding position, (F) ) Is a cross-sectional view in which the reagent container is arranged in the reagent container housing portion at the second holding position. It is the figure which expanded and showed the container part for air suction and the container for air suction of the Example, (A) is a top view of the container part for air suction, (B) is FF position of (A) (C) is a plan view of the air suction container, (D) is a cross-sectional view at the GG position of (C), and (E) is the air suction container accommodating portion. Sectional drawing arrange | positioned in the 1st holding position, (F) is sectional drawing which has arrange | positioned the air suction container in the air suction container accommodating part in the 2nd holding position. It is the schematic sectional drawing which showed the reaction processing apparatus for processing a reaction container plate with the reaction container plate. It is a top view for demonstrating the operation | movement which introduce | transduces a sample liquid into a reaction container from a sample container. It is a top view for demonstrating the operation | movement following FIG. FIG. 10 is a plan view for explaining the operation following FIG. 9. It is a top view for demonstrating the operation | movement following FIG. It is a top view for demonstrating the operation | movement following FIG. It is a top view for demonstrating the operation | movement following FIG. It is a top view for demonstrating the operation | movement following FIG. It is a schematic sectional drawing which expands and shows the reaction container vicinity of the other Example of the reaction container plate. It is a schematic sectional drawing which expands and shows the reaction container vicinity of the further another Example of the reaction container plate. It is a schematic sectional drawing which expands and shows the reaction container vicinity of the further another Example of the reaction container plate.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Reaction container plate 3 Base plate 5 Reaction container 11 Flow path base 13 Main flow path 15 Metering flow path 17 Injection flow path 19, 21 Reaction container air vent flow path 35 Sample container (sealing container)
35a Sample container flow path (sealing container flow path)
35b Sample container air vent channel 35c Locking claw (sealing container holding mechanism)
35d Protrusion channel 35e Second projection channel 35j Film (penetrable part)
35l film (second penetrable part)
35m, 35n Locking groove (sealing container holding mechanism)
37 Reagent containers (sealing containers)
37a Reagent container flow path (sealing container flow path)
37b Reagent container air vent channel 37c Locking claw (sealing container holding mechanism)
37d Protrusion channel 37e Second projection channel 37j Film (penetrable part)
37l film (second penetrable part)
37m, 37n Locking groove (sealing container holding mechanism)
39 Air suction container (sealing container)
39a Air suction container channel (sealing container channel)
39b Air suction container air vent channel 39c Locking claw (sealing container holding mechanism)
39d Projection channel 39e Second projection channel 39j Film (penetrable part)
39l film (second penetrable part)
39m, 39n Locking groove (sealing container holding mechanism)
41 Septum (elastic member)
53 Bellows (Capacity variable part)
54 Seal plate 55 Flow path drawing plate 55a Fixing member engaging part 73 Flow path spacer 75 Protruding part 77 Injection flow path 79 Reaction vessel air vent flow path 87 Rotary switching valve 87a Cylinder 87b Syringe flow path 87c Plunger 87d Cover body 87g Sealed Space 87h Syringe air vent channel 87i Arc-shaped channel port 87j Syringe port 88 Seal plate 89 Rotor cap 91 Backup ring

Claims (18)

A reaction vessel, a reaction vessel channel connected to the reaction vessel, a sealing vessel provided separately from the reaction vessel, and a base plate provided with a sealing vessel channel connected to the sealing vessel;
The base plate is provided with each withdrawal channel connected to the reaction vessel channel and the sealing vessel channel, and attached to the base plate, and one end connected to the reaction vessel channel and the sealing vessel channel, respectively. The opposite surface is provided with a channel port arrangement part, and the other end of each of the drawer channels is arranged as a channel port connected to the reaction vessel channel and the sealing vessel channel in the channel port arrangement unit, respectively. A flow path drawer plate,
A syringe port placement section is provided that includes a syringe that is rotatably attached to the flow path drawing plate and that sucks and discharges a liquid or a gas by sliding a plunger therein, and a syringe port connected to the syringe. A rotary type switching valve that is provided at a position facing the road port arrangement part, and that the syringe port arrangement part is in contact with the flow path port arrangement part and can be connected by switching the syringe port to each flow path port;
A seal plate made of an elastic material provided between the base plate and the flow path drawer plate;
The reaction vessel, the reaction vessel channel, the sealing vessel and the sealing vessel channel are closed systems,
A reaction vessel plate in which an edge of a channel on the surface of the base plate in contact with the seal plate or an edge of a channel on the surface of the channel extraction plate in contact with the seal plate is raised. A fixing member engaging portion protruding from the surface of the flow channel drawing plate is provided around the flow channel port arrangement portion of the flow channel drawing plate,
The inside of the fixing member engaging portion is in contact with the peripheral portion of the surface opposite to the syringe port arrangement portion of the rotary switching valve and engages with the fixing member engaging portion, and the syringe port arrangement portion The reaction vessel plate according to claim 1, wherein a ring-shaped lower surface side fixing member that presses the channel port arrangement portion on the channel port arrangement portion is attached. The lower surface side fixing member is made of resin and its outer diameter is substantially the same as the inner diameter of the fixing member engaging portion,
A first engaging structure portion that engages with each other at corresponding positions on the outer peripheral surface of the lower surface side fixing member and the inner peripheral surface of the fixing member engaging portion;
3. The reaction according to claim 2, wherein the first engaging structure portion is inclined in a direction in which an outer peripheral surface side surface of the lower surface side fixing member or an inner peripheral surface side surface of the fixing member engaging portion closes to the base plate side. Container plate. The base plate and the flow path drawing plate have a through hole for inserting a part of the rotary type switching valve,
The rotary type switching valve has an insertion portion that is partially inserted into the through hole from the flow path drawing plate side, and has a diameter larger than the insertion portion at the proximal end portion of the insertion portion. A non-insertion portion that is not inserted into the through hole and is in contact with the surface of the flow path drawing plate around the through hole;
The periphery of the through hole of the flow path drawing plate is the flow path port arrangement portion, and the position facing the flow path port arrangement portion of the non-insertion portion of the rotary switching valve is the syringe port arrangement portion. ,
The distal end of the insertion portion of the rotary switching valve is a protruding portion that penetrates the through hole of the base plate and further protrudes to the opposite side of the base plate,
The outer peripheral surface of the protruding portion engages with the outer peripheral surface of the protruding portion while contacting the surface of the base plate around the through hole, and locks the rotary switching valve and pulls the insertion portion in the penetrating direction. The reaction container plate according to any one of claims 1 to 3, wherein a distal end side fixing member is fitted. The tip side fixing member is made of resin and the inner diameter thereof is substantially the same as the outer shape of the protruding portion,
A second engaging structure portion that engages with each other at corresponding positions on the inner peripheral surface of the distal-end-side fixing member and the outer peripheral surface of the protruding portion;
5. The reaction according to claim 4, wherein the second engaging structure portion is inclined in a direction in which an inner peripheral surface side surface of the distal end side fixing member or an outer peripheral surface side surface of the protruding portion closes toward the protruding portion distal end side. Container plate. A first penetrating portion is provided on the bottom surface of the sealing container that can be pressed from the outside and penetrated;
One end of the sealed container channel protrudes upward, and the sealed container channel is connected to the sealed container when the one end of the sealed container channel penetrates the first through portion during use. The reaction container plate according to any one of claims 1 to 5.   When the sealing container is not used, the first penetrating portion is held so as to face the one end of the sealing container flow path, and when used, the one end of the sealing container flow path is connected to the first penetrating section. The reaction container plate according to claim 6, which is held in a penetrating state. A second penetrating portion that can be pressed from outside and penetrated at a position where the sealed container does not touch the liquid contained therein, is provided.
The base plate main body further includes a sealed container air vent channel, one end of which protrudes upward, and one end of which presses and penetrates the second through portion when the sealed container is used. The reaction vessel plate as described.   The reaction container plate according to any one of claims 1 to 8, wherein the sealing container includes a sample container for containing a sample liquid.   The one surface of the sample container is sealed with a sealing member made of an elastic member that can be penetrated by a member having a pointed end and can repair a hole due to penetration by elastic force after the member is pulled out. Reaction vessel plate.   The reaction container plate according to any one of claims 1 to 10, wherein a sample pretreatment liquid or a reagent is previously stored in the sealing container.   The reaction container plate according to any one of claims 1 to 11, wherein the sealing container also includes a gene amplification container for performing a gene amplification reaction. A reaction vessel air vent channel connected to the reaction vessel;
The reaction vessel flow path is a groove formed on a bonding surface of two bonded members, or a main flow path that is formed of a through hole formed in the groove and the substrate, and connected to the syringe; A metering channel having a predetermined capacity branched from the main channel, and an injection channel having one end connected to the metering channel and the other end connected to the reaction vessel,
The main channel and the reaction vessel air vent channel can be sealed,
The injection channel is formed narrower than the metering channel, and the liquid introduction pressure state when the liquid is introduced into the main channel and the metering channel, and the purge when the liquid in the main channel is purged The reaction container plate according to any one of claims 1 to 12, wherein the liquid is not passed in a pressure state and the liquid is passed in a pressurized state. The reaction container plate according to claim 13, wherein a contact angle of the injection channel with respect to the water droplet is 90 degrees or more, and an area of a boundary between the injection channel and the metering channel is 1 to 10000000 μm ² .   The reaction according to claim 13 or 14, comprising a plurality of the reaction vessels, each of the reaction vessels comprising the metering channel and the injection channel, and a plurality of the metering channels connected to the main channel. Container plate.   The other end of the injection channel is disposed at the tip of a convex portion formed to protrude from the inner upper surface of the reaction vessel, and the tip of the convex portion is thinner than the base end portion. The reaction container plate according to any one of 13 to 15.   The reaction container plate according to any one of claims 1 to 16, wherein the reaction container is made of a light-transmitting material so that optical measurement can be performed from the bottom or from above. A reaction treatment method using the reaction container plate according to any one of claims 13 to 17,
Filling the main channel and the metering channel with the introduction pressure,
Discharging the liquid in the main channel while allowing the gas to flow in the main channel and leaving the liquid in the metering channel;
By setting the inside of the main channel to a positive pressure greater than the introduction pressure, the inside of the reaction vessel to a negative pressure, or both the positive pressure and the negative pressure, Injecting the liquid into the reaction vessel.

Images