JP2010095474A - Calcium absorption-promoting composition and calcium absorption-promoting food and drink - Google Patents

Abstract

The present invention provides a new calcium absorption promoting composition capable of promoting calcium absorption by ingestion.
The present invention proposes a calcium absorption promoting composition comprising catechins, particularly catechins mainly composed of ester-type catechins, as active ingredients. By orally ingesting catechins, the absorption efficiency of calcium into the blood can be increased.
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Description

  The present invention relates to a calcium absorption promoting composition capable of promoting absorption of calcium by ingestion orally and a calcium absorption promoting food and drink comprising the same.

  Calcium is the most abundant mineral contained in the body and occupies 1-2% of body weight. 99% of them are present in teeth and bones, and the remaining 1% is present in body fluids such as blood and tissues such as muscles. And 1% of this is involved in hemostasis, nerve and muscle functions, and plays an important role in the body.

The living body is provided with a regulation mechanism for efficiently absorbing calcium from the intestinal tract in order to maintain the blood calcium concentration, and two different mechanisms are provided for calcium absorption from the intestinal tract. One is active transport performed from the duodenum to the upper small intestine, and the other is diffusive transport performed from the lower small intestine.
The former involves active vitamin D and calcium binding protein, and the substance is transported against the concentration gradient. Therefore, although the amount of absorption increases with an increase in calcium intake, the time and distance to pass may be short, and when it reaches a certain amount, it reaches a peak.
On the other hand, in diffusion transport, in addition to various factors related to absorption, since the transit time and distance are long, the absorption amount tends to increase as the calcium intake increases.

  The results of recent national health and nutrition surveys have pointed out that the current Japanese diet does not provide sufficient calcium supplementation, and improvements in diet are desired. However, it is actually very difficult to replenish enough calcium by improving and continuing diet. Therefore, if the ingested calcium can be absorbed more efficiently than before, it becomes possible to establish a dietary habit that is easy to continue. From such a viewpoint, development of the substance and composition which accelerates | stimulates calcium absorption is calculated | required.

  Conventionally, vitamin D and lactose are known as ingredients that promote calcium absorption, and the following substances and compositions have been disclosed.

  For example, Patent Document 1 discloses a health food and drink that has a calcium absorption promoting effect mainly composed of polyγ-glutamic acid (molecular weight of about 1.25 million) or a salt thereof, which is known as a representative example of polyamino acids produced by Bacillus natto. It is disclosed.

  Patent Document 2 discloses a calcium absorption promoting composition containing water-soluble chitosan as an active ingredient, and Patent Document 3 discloses a calcium absorption promoting pharmaceutical composition containing soy milk and casein. 4 discloses a calcium absorption-promoting composition containing, as an active ingredient, a calcium absorption-promoting water-soluble fraction obtained by pepsin treatment of walleye pollack or krill-derived protein.

  Patent Document 5 discloses a calcium absorption promoter characterized by comprising a peptide comprising a degradation product of royal jelly by protease as an active ingredient, and Patent Document 6 discloses κ-casein glycomacropeptide and / or A calcium absorption promoter containing an enzymatic degradation product of κ-casein glycomacropeptide as an active ingredient is disclosed, and Patent Document 7 discloses an additive for promoting mineral absorption containing high molecular weight chitosan as an active ingredient.

  Further, Patent Document 8 discloses a calcium absorption promoting composition containing a hardly absorbable L-type aldonic acid, a salt thereof, or a hardly absorbable L-type aldonic acid lactone, and Patent Document 9 discloses a mouse rat or an extract thereof. Is disclosed as an active ingredient, and Patent Document 10 discloses a calcium absorption enhancer containing Ansokuko or its extract as an active ingredient.

  In addition, galactooligosaccharide (Patent Document 11), fructooligosaccharide (Patent Document 12), α-glucosidase inhibitor (Patent Document 13), and the like are also disclosed as substances that promote absorption of calcium in the digestive tract.

Patent 3112724 claim 1 and [0002] Claim 1 of Japanese Patent No. 3393560 Claim 1 of Japanese Patent No. 3280420 Claim 2 of Japanese Patent No. 3397258 Claim 1 of Japanese Patent No. 34083939 Claim 1 of Japanese Patent No. 3575724 Claim 1 of Japanese Patent No. 4062650 Claim 1 of JP-A-2006-70028 Claim 1 of JP2008-74818 Claim 1 of JP-A-2008-74819 Japanese Patent No. 3179090 JP 7-252156 A Japanese Patent No. 2531273

  The present invention is intended to provide a new calcium absorption promoting composition which can promote absorption of calcium by ingestion.

  The present invention proposes a calcium absorption promoting composition comprising catechins, particularly catechins mainly composed of ester-type catechins, as active ingredients.

  Conventionally, catechins are known to lower the iron ion concentration in the blood, and catechins have been considered unsuitable for absorption of minerals such as calcium. However, the present inventor has found an effect capable of enhancing the absorption efficiency of calcium into blood by ingesting catechins orally, and has conceived the present invention.

  The calcium absorption promoting composition of the present invention can be supplemented and provided, but it is provided as a food or drink having a calcium absorption promoting effect by adding it to foods and beverages (collectively referred to as “food and drink”). You can also In addition, it can be provided as a food or drink having a more preferable calcium absorption promoting effect by blending with calcium or a food or drink containing calcium, and it is lost even to a growing child or an elderly suffering from osteoporosis or lost by exercise. It is possible to provide a product suitable for calcium supplementation as a mineral.

BEST MODE FOR CARRYING OUT THE INVENTION

  Hereinafter, although embodiment of this invention is described, this invention is not limited to the following embodiment.

  The calcium absorption promoting composition according to this embodiment (hereinafter referred to as “the present Ca absorption promoting composition”) contains catechins as an active ingredient.

  The catechins which are the active ingredients of the present Ca absorption promoting composition are (-) catechin (C), (-) catechin gallate (Cg), (-) gallocatechin (GC), (-) gallocatechin gallate (GCg), One or more selected from the group consisting of (-) epicatechin (EC), (-) epicatechin gallate (ECg), (-) epigallocatechin (EGC), (-) epigallocatechin gallate (EGCg) It is a mixture consisting of

  Among them, from the viewpoint of the calcium absorption promoting effect, ester-type catechins, that is, (−) catechin gallate (Cg), (−) gallocatechin gallate (GCg), (−) epicatechin gallate (ECg), (−) epigallo It is preferable that the main component is a mixture composed of one or a combination of two or more selected from the group consisting of catechin gallate (EGCg). In particular, it is preferable to use, as an active ingredient, catechins in which the ester type catechins account for 20 to 100% by mass, particularly 40 to 100% by mass, particularly 70 to 100% by mass of the total amount of catechins.

Among these, since (−) epigallocatechin gallate (EGCg) is excellent in calcium absorption promoting action, those containing EGCg as an active ingredient are preferable.
In addition, since the derivative and polymer of each catechin mentioned above can anticipate the same effect, it can be considered that these can also be used like each catechin. Therefore, unless otherwise specified, the expression “catechins” encompasses both the intention to include derivatives and polymers, and more preferably the intention to include catechins that do not include derivatives and polymers.

The catechins as active ingredients of the present Ca absorption promoting composition are preferably catechins isolated and purified from tea extracts and the like, but tea extracts containing catechins in high concentrations are also useful in the present Ca absorption promoting composition. It can be used as an active ingredient. However, there are research reports that people who are deficient in calcium intake bone mass when large amounts of caffeine are consumed (Harris SS et al., Am J Clin Nutr 60: 573-578,1994). Considering the effect on caffeine hypersensitivity and symptoms such as inability to sleep at night, caffeine content is preferably low, preferably 0.5% by mass or less. .
From such a point of view, for example, an extract obtained by hot water extraction treatment of green tea is separated and dried in an adsorption column using water and low / high concentration alcohol, and the tea polyphenol concentration is about 85 to 99.99. A green tea extract prepared to 5% can be preferably exemplified, and among these, “Tearfuran 90S (trade name; manufactured by ITO EN)” is a preferred example. In this theafuran 90S, the amount of ester catechins is 50 to 90% by mass relative to the total amount of catechins, the amount of EGCg is 40 to 90% by mass of the total amount of catechins, and the caffeine content is also the same as the total amount of catechins. 0 to 2% by mass.

This Ca absorption promotion composition can be provided as a pharmaceutical, a quasi-drug, or a dietary supplement (supplement) as an oral administration agent.
At this time, the dosage form can be prepared, for example, in the form of a liquid, tablet, powder, granule, sugar-coated tablet, capsule, suspension, emulsion, pill and the like.
Regarding formulation (formulation), commonly used excipients, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, dispersants, buffers, preservatives, solubilizers, It can be produced by a conventional method using a preservative, a flavoring agent, a soothing agent, a stabilizer and the like. Also, for example, lactose, fructose, glucose, starch, gelatin, magnesium carbonate, synthetic magnesium silicate, talc, magnesium stearate, methylcellulose, carboxymethylcellulose or salts thereof, gum arabic, polyethylene glycol, syrup, petrolatum, glycerin, ethanol, propylene Non-toxic additives such as glycol, citric acid, sodium chloride, sodium sulfite, and sodium phosphate can be blended.
In addition, when preparing as a quasi-drug, it can make it easy to ingest, for example by setting it as drinking forms, such as a bottle drink, or forms, such as a tablet, a capsule, and a granule.

Moreover, by adding this Ca absorption promotion composition to various foods and drinks, health foods, health drinks, foods for specific health use, functional foods, and the like having a Ca absorption promotion effect can be prepared.
For example, carbon dioxide, excipients (including granulating agents), diluents, or sweeteners, flavors, flours, starches, sugars, oils and other various proteins, carbohydrate raw materials, vitamins, minerals and other food materials Mixed with one or more selected, or currently known foods and beverages such as sports drinks, fruit drinks, milk drinks, tea drinks, vegetable juices, dairy drinks, alcoholic drinks, jelly, jelly drinks, carbonic acid It can be produced by adding to beverages, chewing gum, chocolate, candy, biscuits, snacks, bread, dairy products, fish paste products, livestock meat products, frozen desserts, dried foods, supplements and the like.

Since the present Ca absorption promoting composition is considered to exhibit the effect even more when it is taken together with Ca, it is possible to further enhance the calcium absorption promoting effect by mixing with calcium and foods and drinks containing calcium. It can be provided as a food or drink.
In this case, calcium may be one that can be supplied in a solubilized state, such as citrate, sulfate, gluconate, lactate, bis-glycinate, amino acid chelate, carbonate, oxide, Examples thereof include hydroxide, chloride, phosphate, pyrophosphate, acetate, fumarate, malate, and the like. Among these, calcium citrate malate, calcium gluconate, calcium lactate, calcium lactate gluconate, calcium chloride and the like are preferable from the viewpoint of Ca absorption promoting effect. Among them, those containing lactic acid such as calcium lactate and calcium lactate gluconate are preferable from the viewpoint of Ca absorption promoting effect. At this time, the amount of catechins relative to 100 parts by mass of Ca is preferably 300 to 1100 parts by mass, more preferably 400 to 900 parts by mass, and particularly preferably 500 to 700 parts by mass.

(Required intake and content)
About the active ingredient density | concentration in this Ca absorption promotion composition, when an active ingredient is catechin, it is preferable that it is 10-100 mass%. Among these, 70 to 100% by mass is more preferable.
Moreover, when an active ingredient is ester type catechin, it is preferable that it is 15-100 mass%. Especially, it is preferable that it is 60-100 mass%.
Furthermore, when an active ingredient is EGCg, it is preferable that it is 10-100 mass%. Among these, the content is more preferably 50 to 100% by mass.

Regarding the amount of active ingredient relative to the amount of Ca to be ingested, when the active ingredient is catechins, it is preferably 300 to 3000% by mass of Ca, particularly 300 to 2000% by mass, especially 350 to 1000% by mass. More preferably.
Moreover, when an active ingredient is ester type catechin, it is preferable that it is 300-1500 mass% of Ca, It is 450-1050 mass% especially, It is more preferable that it is especially 650-850 mass% especially.
Moreover, when an active ingredient is EGCg, it is preferable that it is 300-1100 mass% of Ca, 400-900 mass% is especially preferable, and it is still more preferable that it is especially 500-700 mass% especially.

  Regarding the safety of the present Ca absorption promoting composition, the catechins that are active ingredients are ingredients extracted from tea that have been orally ingested by humans for many years, so safety is guaranteed. It can be said.

(Explanation of terms)
In the present specification, when expressed as “X to Y” (X and Y are arbitrary numbers), unless otherwise specified, “preferably greater than X” and “preferably Y” together with the meaning of “X to Y”. It means “smaller”. Further, X and Y at that time are numerical values considering rounding.
Further, when expressed as “X or more” or “Y or less” (X and Y are arbitrary numbers), it means “preferably larger than X” or “preferably smaller than Y” unless otherwise specified. To do. Further, X and Y at that time are numerical values considering rounding.

  Next, although this invention is further demonstrated based on a test example, this invention is not limited to the Example shown below.

<Calcium Absorption Test of Green Tea Catechin Using Rats>
Using rats, the effect of simultaneous intake of green tea catechin and calcium on calcium absorption was examined.

  In this test, rats (SPF) Slc: SD male 10-week-old (body weight 311 to 341 g) were fasted from 17:00 on the day before administration of the test substance, and the test was performed.

  As green tea catechins, Tea Flan 90S (hereinafter referred to as “TF90S” manufactured by ITO EN) and epigallocatechin gallate (EGCG) were used. As the calcium source, calcium lactate pentahydrate (molecular weight: 308.30) was used. Table 1 shows the composition of theafuran 90S used.

The test substance was forcibly orally administered (10 mL / kg body weight / single administration) using an oral sonde and a syringe.
Each test substance was prepared by the following method and used as a sample. See Table 2 below for details.
The TF90S + calcium lactate administration liquid was prepared by weighing TF90S1740 mg and calcium lactate 1170 mg, respectively, and dissolving each with water for injection using a pestle and mortar to make a total volume of 15 mL. This was TF90S1160 mg / kg + calcium lactate 780 mg / kg As adjusted.
EGCG + calcium lactate administration solution was prepared by weighing EGCG 879 mg and calcium lactate 1170 mg, respectively, using pestle and mortar to dissolve in water for injection to a total volume of 15 mL, EGCG 580 mg / kg + calcium lactate 780 mg / kg .
For the calcium lactate administration solution, 1170 mg of calcium lactate was weighed and dissolved in water for injection using a pestle and mortar to make a total volume of 15 mL, and this solution was prepared as a calcium lactate 780 mg / kg administration solution.

In the test, the test substance is orally administered to rats, blood is collected before administration, at 0.5, 1, 2 and 4 hours after administration, and the Ca concentration in the blood is measured. evaluated.
In the measurement of Ca in the blood, 0.5 mL blood was collected from the jugular vein at 0.5 hours, 1 hour, 2 hours, and 4 hours after administration of each test substance, and the collected blood was collected at 4 ° C. and 1700 × g. The resulting plasma was measured by the MXB method.
Each measured value was expressed as an average value ± standard error. For each measurement item, the Paired t test was performed using the pre-dose value as a control.
About Ca concentration, the test was implemented between two groups of A1 group and A4 group, A4 group and A2 group, and A4 group and A3. The statistical method showed the uniformity of variance by F-test, so Student's t-test was performed. Tukey Type multiple comparison test was performed among the three groups of A4, A2 and A3. Significance levels are expressed as 5% and 1%.

  Table 2 shows the test conditions of each test group, Table 3 shows the administration components of each test group, and Table 4 shows the administration component composition of each test group (molar ratio with Ca, molar ratio with lactic acid). showed that. Table 5 shows the Ca concentration in the blood for each elapsed time for each test group.

(result)
In all cases, there were no deaths or abnormal general conditions during the study period. The influence on the calcium concentration was as follows (Table 5).
The Ca concentration in the blood of the group administered with water for injection was 9.4 mg / dL before administration, whereas 8.9, 8 and 8 hours after administration, respectively, 8.9, 8 0.6, 8.4 and 8.6 mg / dL. Significant decreases were observed at all 0.5 hours, 1 hour, 2 hours and 4 hours after administration, compared to the pre-dose values.
The Ca concentration in the blood of the TF90S + calcium lactate administration group was 9.5 mg / dL before administration, whereas it was 10.0, 10 at 0.5 hours, 1 hour, 2 hours and 4 hours after administration, respectively. 9.9, 9.7 and 8.5 mg / dL. Compared to the pre-dose value, a significant decrease was observed 4 hours after administration. Significantly higher values were observed at 1 and 2 hours after administration compared to the calcium lactate administration group.
The Ca concentration in the blood of the EGCG + calcium lactate administration group was 9.4 mg / dL before administration, whereas 9.3, 10 at 0.5 hours, 1 hour, 2 hours and 4 hours after administration, respectively. 9.9, 9.8 and 8.4 mg / dL. Compared to the pre-dose value, a significant increase was observed 1 hour after administration, and a significant decrease was observed 4 hours after administration. Significantly higher values were observed at 1 and 2 hours after administration compared to the calcium lactate administration group.
In the calcium lactate administration group, the Ca concentration in the blood was 9.3 mg / dL before administration, whereas 9.4 and 9.4 hours after administration, respectively 0.5 hours, 1 hour, 2 hours and 4 hours. 5, 8.7 and 8.3 mg / dL. A significant decrease was observed 4 hours after administration compared to the pre-dose value. Significantly higher values were observed 1 hour after administration compared to the water-administered group.

  From the above, the TF90S + calcium lactate administration group and the EGCG + calcium lactate administration group showed significantly higher Ca concentrations at 1 hour and 2 hours after administration than the calcium lactate alone administration group, and the green tea extract Theafuran 90S and It was revealed that epigallocatechin gallate promotes the absorption of Ca and significantly increases the Ca concentration in the blood.

Claims (5)

  A calcium absorption promoting composition containing catechins as an active ingredient.   The catechin is one or a mixture of two or more selected from the group consisting of catechin, catechin gallate, gallocatechin, gallocatechin gallate, epicatechin, epicatechin gallate, epigallocatechin, epigallocatechin gallate The calcium absorption promoting composition according to claim 1.   The calcium absorption promoting composition according to claim 2, wherein the catechins occupy 50 to 100% by mass of the total amount of the catechins with ester-type catechins.   Caffeine content is 0.5 mass% or less, The calcium absorption promotion composition in any one of Claims 1-3 characterized by the above-mentioned. A calcium absorption promoting food or drink comprising the calcium absorption promoting composition according to any one of claims 1 to 4.