JP2010043036A - Saccharometabolism promoter - Google Patents

Abstract

[Problem] To provide a sugar metabolism promoter that is derived from natural products, is safe even when taken for a long time, has few side effects, and can efficiently prevent visceral fat accumulation and prevent metabolic syndrome.
A sugar metabolism promoter comprising a ground or extract of a plant of the genus Salacia. In more detail, the plant belonging to the genus Salacia is at least one selected from Salacia oblonga , S. reticulata , S. chinensis , S. prinoides. is there.
[Selection figure] None

Description

  The present invention relates to a sugar metabolism promoter containing a powder or extract of a plant belonging to the genus Salacia, and a food or pharmaceutical comprising the component.

  The roots and trunks of Salacia plants have been used as natural drugs in traditional medicine Ayurveda in India and Sri Lanka. In Sri Lanka, the root bark of Salacia reticulata is said to be effective for the treatment of rheumatism, gonorrhea and dermatoses, and for the treatment of early diabetes. In India, the roots of Salacia oblonga are used for the same treatment, and Salacia chinensis is also used for the treatment of diabetes. Has been reported (Non-patent Document 1).

  Furthermore, there is a report that the component contained in Salacia suppresses gluconeogenesis in the liver and has a hypoglycemic hypoglycemic effect (Patent Document 1). It was not obtained.

In recent years, it has been a problem that excessively ingested sugars and lipids are converted into visceral fat, which causes a metabolic syndrome. In order to save this visceral fat, it is more efficient to burn it before it is converted into a lipid that is less metabolized.
However, there have been no reports on materials that have the effect of selectively promoting sugar combustion and sugar metabolism.
Therefore, at present, countermeasures against metabolic syndrome are becoming a social problem, and there is a demand for the development of a safe material having a glucose metabolism promoting action.
JP 2006-290860 A FOOD Style 21, Vol. 6, No. 5, pp. 72-78

  The problem to be solved by the present invention is a sugar metabolism promoter that promotes the metabolism of sugar, effectively prevents the accumulation of visceral fat and prevents metabolic syndrome, which is safe from natural products even with long-term use and has few side effects Is to provide.

In the present invention, as a result of intensive studies to solve the above problems, it has been found that sugar metabolism can be selectively increased by ingesting a powder or extract of a plant of the genus Salacia.
The present invention has the following configuration.
<1>
A sugar metabolism promoter comprising a ground product or extract of a plant of the genus Salacia.
<2>
Salacia plant, Salacia Oburon moth (Salacia oblonga), Salacia retinyl queue alerter (S. reticulata), Salacia chinensis (S. chinensis), is at least one selected from the Salacia Purinoidesu (S. prinoides) The sugar metabolism promoter according to <1>, which is characterized.
<3>
A food containing the sugar metabolism promoter according to <1> or <2>.
<4>
A food material containing the sugar metabolism promoter according to <1> or <2>.
<5>
A pharmaceutical comprising the sugar metabolism promoter according to <1> or <2>.

This time, we investigated the respiratory quotient when ingesting Salacia and found that Salacia intake significantly increased glucose metabolism.
Therefore, according to the present invention, sugar metabolism can be increased and visceral fat accumulation can be prevented. This provides a sugar metabolism promoter that prevents metabolic syndrome and is derived from natural products that are safe even after long-term use and has few side effects.

<Salacia>
The sugar metabolism promoter of the present invention contains a ground product or extract of a plant of the genus Salacia.
As plants of the genus Salacia, plants of the genus Salacia are used, such as Salacia reticulata , S. chinensis , S. prinoides , S. oblonga, etc. be able to. In particular, it can be suitably used Salacia reticulata (S. reticulata), also called Salacia reticulata.

In the present invention, an extract or pulverized product from at least one selected from the group consisting of a stem, root bark and leaves of a plant belonging to the genus Salacia is preferably used.
The leaves are preferably crushed and used as strips or powder. It can also be taken as is.
The root bark and trunk can be used as a powder. It can also be used for extraction of extracts. The extract here refers to an extract of a plant of the genus Salacia. When used for extraction of the extract, it may be used as it is, or the extract can be extracted after being pulverized into fine pieces or powder.

In the present invention, the extract of the plant belonging to the genus Salacia may be in the form of a filtrate after extraction, in a concentrated or diluted state, in the form of a dry powder thereof, or in the form of a mixture thereof.
The dry extract powder of the extract can be used as it is or after being dissolved in a suitable solvent. The solvent may be any solvent that can be used at the time of extraction as long as it does not adversely affect the human body when it remains in the drug or food after preparation. Usually, water, alcohol, hydrous alcohol is preferably used. Use. More preferably, hot water, ethanol or hydrous ethanol is used. The alcohol concentration of the hydrous alcohol may be 30 to 90% by mass, preferably 40 to 70% by mass. Examples of the drying method include spray drying and freeze drying, but are not limited thereto.
The ground powder of the root bark and trunk and the powdered extract powder are preferably 10% or less and more preferably 8% or less in weight loss according to the 15th revised Japanese Pharmacopoeia. More preferred.

Further, the extract or pulverized product of the genus Salacia can be concentrated and dried to be used as a paste or powder. When the extract is concentrated and dried to form a paste or powder, a freeze-drying method, a spray-drying method, or the like is used, but is not limited thereto. Pasta and powdery extracts of Salacia plants can be taken as they are, and drinks such as water, tea, coffee, juice and alcohol, and general foods such as cakes, etc. It is preferable to add 0.01% to 90% by mass of the dry extract powder of the extract of the plant belonging to the genus Salacia, and mix to make a food, more preferably 1.0% to 80% by mass. Hereinafter, it is more preferably added in an amount of 3.0 to 60% by mass. It can also be used for external purposes.
In particular, the drink is preferably a packaged beverage. When the appearance of a packaged beverage is stored for a long time, the color tone changes greatly, which makes it unsuitable as a product. The beverage is gradually colored, and the color changes over time.
In order to maintain the color tone, further effects can be exhibited by adding an antioxidant such as ascorbic acid or sodium ascorbate. The blending amount of the antioxidant is about 0.03 to 1.2% by mass of the content composition, preferably about 0.04 to 1.0% by mass, more preferably about 0.05 to 0.8% by mass. is there.
More preferably, (small an IC ₅₀ value) high sucrase inhibitory activity genus Salacia powder or extracts of the tablet calcium carbonate or silicon dioxide containing plant that allowed to contain 1% or more of the total weight, with time Provide a method to reduce the burden on the ingestor by preventing the discoloration caused by odors and reducing the required intake. In addition, since the amount used is small, the range of uses in manufacturing and processing is widened, and the cost of products can be suppressed.

  Moreover, it is preferable that 0.0001 mass% or more and 3 mass% or less of mangiferin is contained with respect to the total amount of the sugar metabolism promoter.

The content of mangiferin can be measured as follows.
Measurement of Mandiferin Content Mandiferin content is measured by the following method using HPLC.
<HPLC conditions>
Column: Capcellpack C18 UG120 φ4.6 × 250mm (Shiseido)
Column temperature: 40 ° C
Detection: UV360
Flow rate: 1.0mL / min
Solvent A: 1.0% Acetic acid Solvent B: Methanol Linear gradient (% B): 15% (0min) → 25% (20min)
Samples are prepared by dissolving in 50% methanol and removing insolubles with a syringe filter.
The content is calculated from the detected peak area of mangiferin using a standard curve of the standard.

The sugar metabolism promoter of the present invention preferably has a sucrase 50% inhibitory concentration (IC ₅₀ value) of 50 μg / ml or more and 800 μg / ml or less. When the inhibitory activity is smaller than this range, the action of suppressing the absorption of glucose from the digestive tract is weakened, the predetermined effect is slightly weakened, and when the inhibitory activity is increased, abdominal fullness and gas generation are slightly strengthened. The sucrase 50% inhibitory concentration is preferably 80 μg / ml or more and 600 μg / ml or less, more preferably 100 μg / ml or more and 450 μg / ml or less.
The sucrase 50% inhibitory concentration (IC ₅₀ value) is measured by the following method.

· Preparation of Measurement Sample solution sucrase _{an IC 50} value: Weigh a sample of 2mg the tube (glucose uptake-promoting agent), and suspended well by the addition of water 2 mL, to create a sample solution of 1 mg / mL concentration. This is diluted with water to 0, 50, 100, 250, and 500 μg / mL, respectively.
Preparation of substrate solution: Sucrose is dissolved in 0.2 M maleate buffer (pH 6.0) to a sucrose concentration of 100 mM, and this is used as a substrate solution.
Preparation of the crude enzyme solution: 1 g of intestine acetone powder rat (manufactured by SIGMA) is suspended in 10 mL of physiological saline, followed by centrifugation (3,000 rpm, 4 ° C., 5 min). The obtained supernatant is separated to obtain a crude enzyme solution.
400 μL of the substrate solution is added to 500 μL of the sample solution of each concentration described above, and pre-warmed for 5 minutes at 37 ° C. in a water bath. 100 μL of the crude enzyme solution is added thereto, and the mixture is reacted at 37 ° C. for 60 minutes. After completion of the reaction, the enzyme is deactivated by heating at 95 ° C. for 2 minutes to stop the reaction. The produced glucose concentration is quantified using a commercially available kit, mutarotase / glucose oxidase method (glucose CII test Wako, Wako Pure Chemical Industries, Ltd.).
Blank preparation: 200 μL of substrate solution and 50 μL of crude enzyme solution are added to 250 μL of the sample solution of each concentration described above, and the enzyme is heat-inactivated by immediately heating at 95 ° C. for 2 minutes to obtain blank data. .
A calibration curve is prepared from the obtained values, and the concentration at which the enzyme activity is inhibited by 50% (IC ₅₀ value) is determined.

-Sugar metabolism promotion By taking the sugar metabolism promoter of the present invention, the metabolism of sugar can be promoted in the body.
In animals including humans, metabolism as an energy generation source mainly includes sugar metabolism by glycolysis and lipid metabolism by β oxidation of fatty acids. The balance between sugar metabolism and lipid metabolism can be determined, for example, by obtaining a respiratory quotient (RQ). Specifically, RQ approaches 1.0 when sugar metabolism is mainly used, and RQ approaches 0.7 when lipid metabolism is mainly performed.

<Other inclusions>
The sugar metabolism promoter of the present invention preferably contains a flavonoid in addition to the extract or pulverized product of the genus Salacia.
Flavonoids are a general term for pigment components present in all organs of plants, and are mainly contained in fruits and vegetables, and are particularly present in the form of glycosides in the leaves of green leaves, white vegetables and citrus fruits.
In the present invention, the flavonoid is a general term for pigment components widely contained in plants, and particularly means a flavan derivative contained in a large amount in vegetables and fruits.

Flavonoids are preferably flavonols, isoflavones and catechins. Flavonols are known as polyphenols.
Flavonoids are substances ingested by the body, but are generally difficult to absorb. However, since flavonoids are effective even in a small amount and are strong antioxidants, it is known that they inhibit the activity of carcinogens, have blood circulation promoting action and antithrombotic action.

In the present invention, flavonoids can be obtained from various sources such as tea, grapes and onions. Here, the term “derived” means a substance extracted from at least a part of the living body. For the extraction, for example, a method for preparing the extract of the above-mentioned Salacia plant is applied, and the form of the extract can be the same as described above, for example, with the filtrate after extraction, or concentrated or diluted. It may be in the form of a dried powder or a dry powder thereof, or a mixture thereof.
A tea extract containing catechins is prepared from a tea tree, which is an evergreen tree of the camelliaceae family. The tea tree used is either Assamika cultivated in India, Sri Lanka or Southeast Asia, or Camellia sinensis cultivated in China or Japan. In the extraction, usually, water, alcohol or hydrous alcohol is preferably used. More preferably, hot water, ethanol or hydrous ethanol is used as the extraction solvent. The alcohol concentration of the hydrous alcohol may be 30 to 90% by mass, preferably 40 to 70% by mass. Examples of the drying method include spray drying and freeze drying, but are not limited thereto.

The tea extract contains antioxidants such as polyphenols and catechins. Catechin, epicatechin, gallocatechin, epigallocatechin, catechin gallate, epicatechin gallate, gallocatechin gallate or epigallocatechin gallate is preferably contained, and epigallocatechin gallate is particularly preferred.
The sugar metabolism promoter of the present invention preferably contains 0.1 to 40% by mass of the tea extract, more preferably 0.5 to 35% by mass, and 1.0 to 30% by mass. It is particularly preferable to do this.

In addition, flavonols, which are one of flavonoids, remove active oxygen and exhibit antioxidant effects such as suppression of arteriosclerosis and improvement of blood flow. Among the flavonols, resveratrol, one of polyphenols, has attracted attention as an antioxidant. Resveratrol is composed of a stilbene skeleton, and is contained in grape skins, and is therefore also contained in red wine made from grapes.
It is preferable that this invention contains the grape extract or wine concentrate which contains this flavonol as a component.
The sugar metabolism promoter of the present invention preferably contains 0.1 to 30% by mass of grape extract, and more preferably 0.1 to 10% by mass.

  Resveratrol has the function of burning fat, preventing arteriosclerosis, which is a disease of the vascular system, preventing cancer, shortening due to DNA cell division, and prolonging cell life similar to that of calorie restriction. It is effective and is known to have excellent effects as a lifestyle-related disease prevention material.

  The content of resveratrol in the sugar metabolism promoter of the present invention is preferably 0.0001 to 5.00% by mass, and more preferably 0.001 to 2.00% by mass.

  Among flavonols, quercetin, which is a polyphenol, has attracted attention as an antioxidant. Quercetin has a flavan structure and is abundant in the onion skin.

  Quercetin has been reported to have physiological effects such as vitamin C absorption support, antioxidant action, immune action, etc., and it has been shown to be effective in suppressing fat absorption, and has excellent effects as a lifestyle-related disease prevention material. I know I have

  The content of quercetin in the sugar metabolism promoter of the present invention is preferably 0.001 to 15% by mass, more preferably 0.05 to 10% by mass, and further 0.1 to 5.0% by mass. preferable.

The sugar metabolism promoter of the present invention preferably contains 1 to 50% by mass of catechin. As catechins, those derived from green tea are particularly preferred.
Moreover, it is preferable that the sugar metabolism promoter of this invention contains 2-80 mass% of polyphenols which have a lipase activity inhibitory effect. As polyphenols having a lipase activity inhibitory effect, those derived from oolong tea, grapes, apples, lychees, pine bark, kanka and the like are particularly preferred.

  The sugar metabolism promoter of the present invention preferably contains 20 μg to 200 μg of trivalent chromium as a daily intake. Trivalent chromium can be blended as chromium picolinate or chromium yeast.

<Form>
The sugar metabolism promoter of the present invention can also be used as food (for example, beverages, confectionery, so-called health supplements), food raw materials (such as additives added to foods), and pharmaceuticals. In addition, the sugar metabolism promoter of the present invention can take various forms such as powder preparations, tablets, liquid preparations, capsule preparations and the like.

In the present invention, in order to improve discoloration of the extract extract powder of Salacia plant over time, it contains 1% or more and 95% or less of the mass when calcium carbonate or silicon dioxide is made into a tablet or capsule as a desiccant. Is preferred.
Furthermore, low moisture-absorbing raw materials, hygroscopic agents, antioxidants and the like that can be used as foods or food additives can be used. Preferably, cellulose, crystalline cellulose, powdered cellulose, microcrystalline cellulose, lactose, oligosaccharide, sugar alcohol, trehalose, magnesium stearate, calcium stearate and the like are used as the low hygroscopic raw material. As the hygroscopic agent, silicates, magnesium carbonate, ferrocyanide, polysaccharides and the like are used. More preferably, crystalline cellulose, microcrystalline cellulose, or lactose is used as the low hygroscopic raw material. As the antioxidant, ascorbic acid or sodium ascorbate is used.

  A compound necessary for molding into the powder, solid agent or liquid agent of the present invention may be appropriately included. Examples of such compounds include erythritol, maltitol, hydroxypropylcellulose, kaolin, talc and the like.

In the present invention, known means and known materials can be applied to preparations for preparing powder preparations, tablets or liquids, granulation of capsule inclusions for forming capsule preparations, encapsulation, capsule materials, and the like.
The capsule preparation of the present invention may be in the form of hard capsule, soft capsule, seamless capsule microcapsule, etc., and the capsule film is selected from pork skin gelatin, pork bone gelatin, fish gelatin or natural hydrophilic polymer It is comprised by at least 1 type, or 2 or more types. A capsule skin of pig skin gelatin or fish gelatin is particularly preferred.
These capsule films can be produced by a known and conventional method. Here, pork skin gelatin, pork bone gelatin, fish gelatin or natural hydrophilic polymer means that pork skin gelatin, pork bone gelatin, fish gelatin or natural hydrophilic polymer with respect to the total mass of the capsule film. The total amount is 30% by mass or more, preferably 40% by mass or more, more preferably 50% by mass or more, and particularly preferably 60% by mass or more.
The sugar metabolism promoter is preferably filled in a packaging bag or a packaging container in order to avoid contact with air from the viewpoint of oxidation prevention.

  Hereinafter, the present invention will be described using examples, but the present invention is not limited to the following examples.

(Test Example 1)
Five liters of water was added to 30 g of brown powder obtained by pulverizing the trunk of S. reticulata, and the mixture was boiled and extracted. The liquid obtained through this hot water extraction step was filtered and then dried to obtain a sugar metabolism promoter KTED. The 50% sucrase inhibitory concentration of KTED was 90 μg / ml.
The effect of this sugar metabolism promoter was verified using C57BL / 6J mice (male 4 weeks old) as experimental animals. Henceforth, the value of each group is represented by the average of three mice.
After one week of preliminary breeding, mice in the group corresponding to the test examples (hereinafter referred to as the first group) were mixed with a solid feed for experimental animals (trade name MF manufactured by Oriental Yeast Co., Ltd.) with the above-mentioned glucose metabolism promoter KTED 0.1 % Feed was ad libitum (N + KTED in FIG. 1). Comparative mice (hereinafter N group) were fed the same feed as the first group of mice not mixed with KTED (N in FIG. 1).
The following experiment was performed after raising each for 4 weeks.
Oxygen consumption (VO ₂ ) and respiratory quotient (RQ: carbon dioxide concentration ÷ oxygen concentration) were determined by a small animal metabolism measurement system (Muromachi Kikai Co., Ltd.) every hour for 24 hours.
FIG. 1 shows the oxygen consumption (VO ₂ ) of the 1st and N districts. FIG. 2 shows the respiratory quotient (RQ) of the first county and N county.
From the results of FIG. 1, it was found that VO ₂ was significantly increased by ingesting the sugar metabolism promoter KTED. Further, from the results of FIG. 2, it was clarified that RQ averaged close to 1.0 by intake of this product, and sugar metabolism was further advanced.

(Test Example 2)
As in Test Example 1, C57BL / 6J mice (male 4 weeks old) were used as experimental animals, and after one week of preliminary breeding, mice in the group corresponding to the Examples (hereinafter referred to as Group 2) had a high fat diet. (Oriental Yeast Co., Ltd. product name: F2HFD2) was mixed with 0.1% of the above sugar metabolism promoter KTED. Comparative mice (hereinafter H group) received the same diet as the second group of mice without KTED contamination.
In the same manner as in Test Example 1, the respiratory quotient (RQ) was obtained using a small animal metabolic measurement system.
The results are shown in FIG.
From the results shown in FIG. 3, the effect of ingestion of this product was not seen in the high fat diet intake system, and it was confirmed that the intake of Salacia was related to the burning of sugar.

(Test Example 3)
(Tablets using Salacia extract powder)
Tablets were prepared according to the formulation shown in Table 1, and supplements with shellac coating were prepared.
Red wine polyphenol: Seda herb onion skin extract powder: Taiho Green tea extract: Taiyo Kagaku Hematococcus alga pigment: Fuji Chemical Chrome yeast: Miwa Pharmaceutical Carnitine: Ito Life Sciences

  From subjects taking tablets of this formulation, they reported that their stomachs were clean and their bodies lightened. There were also reports that it was surprisingly difficult to get hangovers.

It is a diagram showing the time course of oxygen consumption of the first gun and the N gun in Test Example 1 (VO _2). It is a figure which shows the time-dependent change of the respiratory quotient (RQ) of the 1st county and N county in the test example 1. FIG. It is a figure which shows the time-dependent change of the respiratory quotient (RQ) of the 2nd county and N county in the test example 2. FIG.

Claims (5)

  A sugar metabolism promoter comprising a ground product or extract of a plant of the genus Salacia. The plant belonging to the genus Salacia is at least one selected from Salacia oblonga , S. reticulata , S. chinensis and S. prinoides. The sugar metabolism promoter according to claim 1, wherein   A food containing the sugar metabolism promoter according to claim 1 or 2.   A food material containing the sugar metabolism promoter according to claim 1 or 2.   A pharmaceutical comprising the sugar metabolism promoter according to claim 1 or 2.

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