JP2009219365A - Nitrogen-feeding device and culture system - Google Patents

Abstract

PROBLEM TO BE SOLVED: To maintain stable environmental conditions of a constant temperature chamber of a culture apparatus and enable stable culture.
A nitrogen supply device for supplying nitrogen gas to a culture device for culturing a sample in a culture vessel in a temperature-controlled room adjusted to a predetermined environmental condition, wherein the nitrogen gas generation unit, A supply unit that supplies the culture apparatus, an operation in which environmental conditions in the temperature-controlled room fluctuate or an environmental condition are determined in advance, an operation or imaging condition is detected, and an acquisition unit that obtains prediction information from the culture apparatus to predict the fluctuation, and prediction And a control unit that controls the supply amount of nitrogen gas by the supply unit according to the information.
[Selection] Figure 1

Description

  The present invention relates to a nitrogen supply device for supplying nitrogen gas to a culture apparatus for culturing a sample of a culture container in a constant temperature room adjusted to a predetermined environmental condition, and a culture container in a constant temperature room adjusted to a predetermined environmental condition The present invention relates to a culture system comprising a culture apparatus for culturing the sample and a nitrogen supply apparatus for supplying nitrogen gas to the culture apparatus.

  Conventionally, a culture apparatus provided with a temperature-controlled room for culturing various microorganisms and cells is generally known (see, for example, Patent Document 1). In such a culture device, the environmental conditions (for example, temperature, humidity, carbon dioxide concentration, oxygen concentration, nitrogen concentration, etc.) of the temperature-controlled room are monitored, and the above-mentioned parameters are adjusted according to changes, thereby controlling the temperature-controlled room. The interior of is maintained at predetermined environmental conditions.

With respect to the culture using such a culture apparatus, in recent years, when culturing a biological sample such as a fertilized egg or ES cell, so-called “low oxygen culture” is carried out by maintaining the oxygen concentration at a concentration below the normal atmosphere. It is thriving.
JP 2004-16194 A

  In the above-described hypoxic culture, it is known that maintenance of the environmental conditions of the constant temperature chamber is particularly important because changes in the environmental conditions of the constant temperature chamber of the culture apparatus may affect the culture of biological samples. ing. Various approaches for maintaining the inside of the temperature-controlled room at a predetermined environmental condition have been conventionally performed as in the above-described known technology. However, in low-oxygen culture, the environmental condition of the temperature-controlled room must be maintained more strictly. It has been demanded.

  An object of the nitrogen supply device and the culture device of the present invention is to maintain the environmental conditions of the temperature-controlled room of the culture device and enable stable culture.

  The nitrogen supply device of the present invention is a nitrogen supply device for supplying nitrogen gas to a culture device for culturing a sample in a culture vessel in a temperature-controlled room adjusted to a predetermined environmental condition, wherein the generating unit generates the nitrogen gas. A supply unit that supplies the nitrogen gas to the culture apparatus; an operation or environmental condition in which the environmental conditions in the temperature-controlled room fluctuate are determined in advance; the operation or imaging conditions are detected; An acquisition unit acquired from the apparatus, and a control unit that controls the supply amount of the nitrogen gas by the supply unit according to the prediction information.

  Preferably, the supply unit includes a buffer tank that stores the nitrogen gas generated by the generation unit, and the control unit supplies the nitrogen gas from the buffer tank according to the prediction information. The amount of nitrogen gas supplied by the supply unit may be controlled by controlling.

  Preferably, the control unit may control the supply amount of the nitrogen gas by the supply unit by controlling the generation amount of the nitrogen gas by the generation unit according to the prediction information.

  The culture system of the present invention is a culture system comprising any one of the above-described nitrogen supply devices and a culture device for culturing a sample of a culture container in a temperature-controlled room adjusted to a predetermined environmental condition. An opening / closing unit capable of taking in and out the culture container, and an information output unit for outputting the opening / closing information of the opening / closing unit to the acquisition unit as the prediction information.

  Another culturing system of the present invention is a culturing system comprising any one of the above-described nitrogen supply devices and a culturing device for culturing a sample of a culturing vessel in a constant temperature room adjusted to a predetermined environmental condition. An apparatus is provided with the information output part which outputs the setting change information of the environmental condition of the temperature-controlled room to the acquisition part as the prediction information.

  Another culturing system of the present invention is a culturing system comprising any one of the above-described nitrogen supply devices and a culturing device for culturing a sample of a culturing vessel in a constant temperature room adjusted to a predetermined environmental condition. An apparatus is provided with the evaluation part which evaluates the environmental condition of the said constant temperature room, and the information output part which outputs the evaluation information by the said evaluation part to the said acquisition part as said prediction information.

  Preferably, the nitrogen supply device includes a determination unit that determines whether the control unit can control the supply amount of the nitrogen gas, and an information output unit that outputs determination information from the determination unit to the culture apparatus. The culture apparatus may include an acquisition unit that acquires the determination information, and a control unit that controls whether the opening / closing unit can be opened or closed according to the determination information.

  Preferably, the nitrogen supply device is installed separately from the culture device, and the culture device includes a first housing and a second housing installed separately from the first housing. The microscope has a sample stage and the temperature-controlled room in the first casing, and the sample placed on the sample stage drive unit and the sample stage in the second casing. A microscope objective lens drive unit for observing the image.

  According to the nitrogen supply device and the culture device of the present invention, it is possible to maintain the environmental conditions of the temperature-controlled room of the culture device and enable stable culture.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

  FIG. 1 is a schematic front view showing the overall configuration of the culture system of the present embodiment. The culture system includes a culture device 1 and a nitrogen supply device 2. The nitrogen supply device 2 is installed separately from the culture device 1. The culture apparatus 1 includes a first housing 10 and a second housing 11. FIG. 2 is a schematic front view showing the overall configuration of the culture apparatus 1.

  The first housing 10 is disposed on the upper side of the second housing 11. A temperature-controlled room 12 covered with a heat insulating material is formed inside the first housing 10. The front side of the first housing 10 forms an opening 10a. And this opening part 10a is obstruct | occluded by the double door front door 13 so that opening and closing is possible. Further, at the position below the right side surface of the first housing 10, a carry-in / out port 15 through which a culture container (well plate, flask, dish, etc.) can pass is formed. The carry-in / out port 15 is closed by an automatic door 17 that is slid by a drive mechanism 16 so as to be opened and closed. Furthermore, an opening (not shown) for arranging a microscope unit 26 described later is formed on the bottom surface of the first housing 10 at a position on the left side when viewed from the front.

  A plurality of temperature control devices using Peltier elements or the like are built in each wall surface inside the temperature-controlled room 12. Since the configuration of the temperature adjusting device is the same as that of the known technology, the description thereof is omitted. Further, inside the temperature-controlled room 12, a humidity adjusting device including a spraying device or the like is provided. Since the configuration of the humidity adjusting device is the same as that of the known technology, the description thereof is omitted. Furthermore, a gas introduction part 21 is disposed on the wall surface inside the temperature-controlled room 12. The gas introduction unit 21 is connected to the nitrogen supply device 2 and an outside air supply device (not shown). And the gas introduction part 21 introduce | transduces gas into the temperature-controlled room 12 from each supply apparatus, and adjusts the carbon dioxide concentration in the temperature-controlled room 12, oxygen concentration, and nitrogen concentration. Furthermore, an internal sensor 22 for detecting environmental conditions inside the temperature-controlled room 12 is disposed on the upper surface inside the temperature-controlled room 12.

  In the temperature-controlled room 12 of the first housing 10, a storage unit 23, a container transport mechanism 24, a container carry-in / out mechanism 25, and a microscope unit 26 are stored.

  The storage unit 23 includes a stocker and the like, and is disposed on the right side in the temperature-controlled room 12 when viewed from the front of the first housing 10. For example, the interior of the storage unit 23 is partitioned vertically by a plurality of shelves. The storage unit 23 can store the culture container horizontally. Further, the lowermost stage of the storage unit 23 is a space for arranging the container carry-in / out mechanism 25.

  In order to facilitate transportation by the container transport mechanism 24, the culture container is placed on a tray-shaped holder or the like and handled, for example. The container transport mechanism 24 is disposed in the center of the temperature-controlled room 12 when viewed from the front of the first housing 10. The container transport mechanism 24 includes a transport arm, a motor, and the like (not shown), and can move the holder on which the culture container is placed in the left-right direction, the up-down direction, and the front-back direction. Therefore, the container transport mechanism 24 can move the holder on which the culture container is placed among the storage unit 23, the container carry-in / out mechanism 25, and the microscope unit 26.

  The container carry-in / out mechanism 25 is installed in the vicinity of the carry-in / out entrance 15 at the lowermost stage of the storage unit 23. The container carry-in / out mechanism 25 includes a motor unit that reciprocates a transfer table on which a holder on which a culture vessel is placed can be placed, to the outside of the carry-in / out entrance 15.

  The microscope unit 26 is arranged on the left side in the temperature-controlled room 12 when viewed from the front of the first housing 10. The microscope unit 26 has a sample stage on which a culture vessel and the like are placed. The microscope unit 26 is disposed by being fitted into the opening on the bottom surface of the first housing 10. The sample stage is arranged in the temperature-controlled room 12 of the first casing 10, but the motor or the like which is a driving mechanism of the sample stage and the Z direction driving mechanism of the objective lens of the microscope unit 26 are on the second casing 11 side. The first housing 10 and the second housing 11 are isolated from each other. The light source of the lighting device is installed in the second housing 11. An optical system that guides light from the light source to the sample stage in the first housing 10 extends from the second housing 11 to the first housing 10. This optical system is configured to be isolated from the first casing 10.

  On the other hand, the main body of the microscope unit 26 and the culture device control unit 40 are stored in the second housing 11. The culture apparatus 1 shown in FIGS. 1 and 2 is an example, and the configuration is not limited to this example.

  Inside the nitrogen supply device 2, a nitrogen supply unit 50 that supplies nitrogen gas to the culture device 1, a nitrogen generation unit 51 that generates nitrogen gas, a buffer tank 52 that stores nitrogen gas, and a supply device control unit that controls each unit 60 is stored.

  The nitrogen supply unit 50 supplies the culture apparatus 1 with nitrogen gas supplied from at least one of the nitrogen generation unit 51 and the buffer tank 52. Note that the nitrogen gas output by the nitrogen supply unit 50 is supplied to the gas introduction unit 21 of the culture apparatus 1. The nitrogen generator 51 generates nitrogen gas in response to an instruction from the supply device control unit 60. The nitrogen gas generated by the nitrogen generating unit 51 is directly supplied to the nitrogen supply unit 50, or is supplied to the nitrogen supply unit 50 after being supplied to the buffer tank 52 (via the buffer tank 52). The The method for supplying nitrogen gas from the nitrogen generator 51 is controlled in accordance with an instruction from the supply device control unit 60. Further, the buffer tank 52 includes a remaining amount sensor (not shown), detects the remaining amount (= stored amount) of nitrogen gas stored therein, and outputs the detected amount to the supply device control unit 60.

  FIG. 3 is a block diagram showing the relationship between the culture device 1 and the nitrogen supply device 2 in the culture system.

  The culture device control unit 40 of the culture device 1 includes a culture device CPU 41, an operation instruction unit 42, and a display panel 43. The culture apparatus CPU 41 is connected to the drive mechanism 16, the gas introduction unit 21, the internal sensor 22, the container transport mechanism 24, the container carry-in / out mechanism 25, and the microscope unit 26. And culture device CPU41 controls the above-mentioned each part according to a predetermined program.

  The operation instruction unit 42 has input means such as a keyboard, and operates each unit of the culture apparatus 1 via the culture apparatus CPU41. That is, the culture apparatus CPU 41 adjusts the environmental conditions in the temperature-controlled room 12 based on the input from the operation instruction unit 42, takes the culture container into and out of the temperature-controlled room 12, observes the sample of the culture container, Perform operations such as transporting the culture vessel at Here, the instruction of the operation instruction unit 42 includes both an instruction by a user's direct input and an instruction set in advance by a program. The display panel 43 outputs and displays the environmental conditions of the temperature-controlled room 12 output from the culture apparatus CPU41.

  The supply device control unit 60 of the nitrogen supply device 2 has a supply device CPU 61. The supply device CPU 61 is connected to the nitrogen supply unit 50, the nitrogen generation unit 51, and the buffer tank 52. The supply device CPU 61 controls each of the above parts according to a predetermined program. The culture device CPU41 and the supply device CPU61 are connected.

  Hereinafter, the operation of the culture system of the present embodiment will be described. First, a general operation of each part of the culture system will be briefly described.

  During the operation of the culture system, the culture apparatus CPU 41 monitors the environmental conditions in the temperature-controlled room 12 using the internal sensor 22. When there is a change in the environmental conditions, the culture apparatus CPU 41 operates any one of the temperature adjustment device, the humidity adjustment device, and the gas introduction unit 21 (not shown) to adjust the environmental conditions in the temperature-controlled room 12 to be constant. In addition, when operating the gas introduction part 21, culture | cultivation apparatus CPU41 gives instruction | indication to supply apparatus CPU61, and operates the nitrogen supply apparatus 2 (it mentions later for details).

  When there is a culture container transport instruction from the operation instruction unit 42, the CPU drives the motor of the container transport mechanism 24 to transport the culture container and the holder on which the culture container is placed. At this time, the container transport mechanism 24 is (1) replacement of the culture container in the storage unit 23, (2) delivery of the culture container to the container carry-in / out mechanism 23, and (3) delivery of the culture container to the microscope unit 26. , Do one of the following.

  When there is an instruction to observe the culture vessel from the operation instruction unit 42, the culture device CPU41 operates the microscope unit 26 to observe the sample in the culture vessel. At this time, the culture device CPU 41 illuminates the sample with the illumination device of the microscope unit 26. Then, the culture apparatus CPU 41 moves the sample stage in the horizontal direction in accordance with an instruction from the operation instruction unit 42. Thereby, the sample can be observed at an arbitrary position of the culture container.

  When there is an instruction to carry out the culture vessel from the operation instruction unit 42, the culture apparatus CPU 41 operates the drive mechanism 16 to open the automatic door 17. Then, the culture apparatus CPU 41 drives the motor unit of the container carry-in / out mechanism 25 to carry out the culture container and holder of the transfer table to the outside of the temperature-controlled room 12. Similarly, when there is a culture container carry-in instruction from the operation instruction unit 42, the culture apparatus CPU 41 drives the motor unit of the container carry-in / out mechanism 25 to carry the culture container and holder of the transfer table into the temperature-controlled room 12. Then, the culture device CPU 41 operates the drive mechanism 16 to close the automatic door 17.

  Next, the operation of the supply device CPU 61 during the operation of the culture system will be described with reference to the flowchart of FIG. Note that a description will be given on the assumption that a constant amount (for example, 3 liters per minute) of nitrogen gas is constantly supplied from the nitrogen generating unit 51 to the gas introducing unit 21 during the operation of the culture system.

  In step S <b> 1, the supply device CPU 61 supplies nitrogen gas from the nitrogen generation unit 51 to the gas introduction unit 21. During normal operation, the supply device CPU 61 supplies nitrogen gas from the nitrogen generation unit 51 to the gas introduction unit 21 without going through the buffer tank 52. The supply amount at this time is controlled in accordance with an instruction from the culture apparatus CPU41.

  In step S <b> 2, the supply device CPU 61 determines whether or not the prediction information has been acquired from the culture device CPU 41. And if it determines with having acquired prediction information, supply apparatus CPU61 will progress to step S3.

  Prediction information is defined in advance as operations and environmental conditions in which the environmental conditions inside the temperature-controlled room 12 are likely to vary, and these operations and environmental conditions are detected to predict the variations, and the culture device CPU 41 outputs them to the supply device CPU 61. Information. When there is an instruction to carry out / carry in the culture container from the outside to the inside of the culture apparatus 1 or from the inside to the outside and the opening / closing of the automatic door 17 is predicted, the culture apparatus CPU 41 (2) an operation instruction When an active change of the environmental conditions inside the temperature-controlled room 12 is predicted based on a user instruction or a recipe to the unit 42, (3) When an abnormality in the environmental conditions inside the temperature-controlled room 12 is detected, In either case, the prediction information is output to the supply device CPU 61.

  In step S <b> 3, the supply device CPU 61 increases the amount of nitrogen gas generated in the nitrogen generator 51. There is a certain range in the amount of nitrogen gas that can be generated in the nitrogen generator 51 (for example, 0 to 20 liters per minute). Accordingly, the supply device CPU 61 increases the amount of nitrogen gas generated in the nitrogen generator 51 in preparation for storing nitrogen gas in the buffer tank 52 described later.

  In step S <b> 4, the supply device CPU 61 starts storing nitrogen gas in the buffer tank 52. The supply device CPU 61 starts storing nitrogen gas in the buffer tank 52 while continuing to supply nitrogen gas from the nitrogen generation unit 51 to the gas introduction unit 21. As a result, nitrogen gas is stored in the buffer tank 52.

  In step S <b> 5, the supply device CPU 61 determines whether a nitrogen supply amount increase instruction has been received from the culture device CPU 41. And if it determines with having received the nitrogen supply amount increase instruction | indication, supply apparatus CPU61 will progress to step S6.

  The nitrogen supply amount increase instruction is information that the culture apparatus CPU 41 outputs to the supply apparatus CPU 61 in order to adjust the environmental conditions inside the temperature-controlled room 12. The culture device CPU41 calculates a nitrogen supply amount necessary for adjusting the environmental conditions inside the temperature-controlled room 12 as an instruction to increase the nitrogen supply amount, and outputs numerical information indicating the calculated nitrogen supply amount to the supply device CPU61. To do.

  In addition, the culture apparatus CPU41 (1) when it is necessary to supply nitrogen gas to the temperature-controlled room 12 by opening and closing the automatic door 17, (2) in order to change the environmental conditions inside the temperature-controlled room 12, When nitrogen gas needs to be supplied to 12, (3) any of the cases where nitrogen gas needs to be supplied to temperature-controlled room 12 in order to normalize abnormal environmental conditions inside temperature-controlled room 12 In this case, a nitrogen supply amount increase instruction is output to the supply device CPU 61.

  In step S <b> 6, the supply device CPU 61 determines whether or not the remaining amount of nitrogen gas is sufficient based on the output of the remaining amount sensor of the buffer tank 52. If it is determined that the remaining amount of nitrogen gas is sufficient, the supply device CPU 61 proceeds to step S7. On the other hand, when determining that the remaining amount of nitrogen gas is not sufficient, the supply device CPU 61 proceeds to step S9 described later.

  The supply device CPU 61 determines whether or not the remaining amount is sufficient based on the numerical value indicating the nitrogen supply amount in the nitrogen supply amount increase instruction received in step S5 and the output of the remaining amount sensor of the buffer tank 52. Do.

  In step S <b> 7, the supply device CPU 61 supplies nitrogen gas to the gas introduction unit 21 from both the nitrogen generation unit 51 and the buffer tank 52. The supply device CPU 61 adjusts each gas pressure and flow rate with respect to the supply amount of nitrogen gas from the buffer tank 52 and the supply amount of nitrogen gas from the nitrogen generation unit 51, so that the nitrogen gas in the buffer tank 52 is supplied. Based on the remaining amount and the amount of nitrogen gas generated in the nitrogen generator 51, the respective supply sharing is appropriately controlled.

  In step S <b> 8, the supply device CPU 61 determines whether a nitrogen supply amount normalization instruction has been received from the culture device CPU 41. And if it determines with having received nitrogen supply amount normalization instruction | indication, supply apparatus CPU61 will return to step S1.

  The nitrogen supply amount normalization instruction is information that the culture apparatus CPU 41 outputs to the supply apparatus CPU 61 in order to return the nitrogen supply amount to the normal state after a predetermined time from the nitrogen supply amount increase instruction described in step S5. When the time necessary for adjusting the environmental conditions inside the temperature-controlled room 12 has elapsed as the predetermined time, the culture device CPU 41 outputs a nitrogen supply amount normalization instruction to the supply device CPU 61.

  In step S9, the supply device CPU 61 transmits error information to the culture device CPU 41. When the error information is received from the supply device CPU 61, the culture device CPU 41 performs at least one of (1) prohibiting the opening and closing of the automatic door 17 and (2) issuing a warning / notification through the display panel 43. Even if the nitrogen gas remaining amount in the buffer tank 52 is insufficient by performing the processing as in (1) and the environmental conditions inside the temperature-controlled room 12 cannot be adjusted due to the opening and closing of the automatic door 17, Further changes in environmental conditions can be prevented. Moreover, the situation regarding the supply of nitrogen gas by the supply device 2 can be notified to the user by performing the process as in (2).

  As described above, according to this embodiment, by controlling the culture apparatus 1 and the nitrogen supply apparatus 2 in conjunction with each other, it is possible to maintain the environmental conditions of the temperature-controlled room of the culture apparatus and to perform stable culture. can do. In particular, it is possible to cope with a situation where more strict maintenance of the environmental conditions of the temperature-controlled room is required, such as low oxygen culture.

  In the present embodiment, the example in which the buffer tank 52 is provided and the nitrogen gas is stored has been described. However, instead of providing the buffer tank 52, a configuration may be provided in which the nitrogen generating unit 51 having a large generation amount is provided. . In this case, instead of supplying nitrogen gas from both the nitrogen generation part 51 and the buffer tank 52 to the gas introduction part 21, the amount of nitrogen gas generated in the nitrogen generation part 51 is greatly increased, thereby achieving the present embodiment. Similar effects can be obtained.

  Moreover, although the automatic door 17 gave the example which opens and closes automatically by the drive mechanism 16 in this embodiment, this invention is applicable similarly regarding a manual door. In this case, an opening / closing sensor may be provided in the door opening / closing section, and a lock mechanism capable of remotely operating the door by the culture apparatus CPU 41 may be provided.

  In the present embodiment, an example in which the culture device CPU 41 of the culture device 1 is the main CPU has been described. However, a CPU of a computer that comprehensively controls the culture device 1 and the nitrogen supply device 2 may be the main CPU. . The supply device CPU 61 of the nitrogen supply device 2 may be the main CPU.

It is a general | schematic front view which shows the whole structure of a culture system. 1 is a schematic front view showing an overall configuration of a culture apparatus 1. FIG. It is a block diagram which shows the relationship between the culture apparatus 1 and the nitrogen supply apparatus 2 in a culture system. It is a flowchart which shows operation | movement of supply apparatus CPU61 at the time of operation | movement of a culture system.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Culture apparatus, 2 ... Nitrogen supply apparatus, 12 ... Constant temperature chamber, 15 ... Carrying in / out port, 16 ... Drive mechanism, 17 ... Automatic door, 21 ... Gas introduction part, 22 ... Internal sensor, 24 ... Container conveyance mechanism, 25 ... Container loading / unloading mechanism, 26 ... microscope unit, 40 ... culture apparatus control unit, 41 ... culture apparatus CPU, 42 ... operation instruction section, 43 ... display section, 50 ... nitrogen supply section, 51 ... nitrogen generation section, 52 ... buffer tank 60 ... Supply device control unit, 61 ... Supply device CPU

Claims (8)

A nitrogen supply device for supplying nitrogen gas to a culture device for culturing a sample of a culture vessel in a thermostatic chamber adjusted to a predetermined environmental condition,
A generating section for generating the nitrogen gas;
A supply unit for supplying the nitrogen gas to the culture apparatus;
Predetermining an operation or environmental condition in which the environmental conditions in the temperature-controlled room fluctuate, detecting the operation or imaging condition, and obtaining prediction information for predicting the fluctuation from the culture device;
A nitrogen supply device comprising: a control unit that controls a supply amount of the nitrogen gas by the supply unit according to the prediction information. The nitrogen supply device according to claim 1,
The supply unit includes a buffer tank that stores the nitrogen gas generated by the generation unit,
The control unit controls the supply amount of the nitrogen gas by the supply unit by controlling the supply amount of the nitrogen gas from the buffer tank according to the prediction information. . The nitrogen supply device according to claim 1,
The said control part controls the supply amount of the said nitrogen gas by the said supply part by controlling the generation amount of the said nitrogen gas by the said generation part according to the said prediction information. The nitrogen supply apparatus characterized by the above-mentioned. A culture system comprising the nitrogen supply device according to any one of claims 1 to 3 and a culture device for culturing a sample of a culture vessel in a thermostatic chamber adjusted to a predetermined environmental condition,
The culture apparatus comprises:
An openable / closable part capable of withdrawing and inserting the culture vessel;
A culture system comprising: an information output unit that outputs the opening / closing information of the opening / closing unit to the acquisition unit as the prediction information. A culture system comprising the nitrogen supply device according to any one of claims 1 to 3 and a culture device for culturing a sample of a culture vessel in a thermostatic chamber adjusted to a predetermined environmental condition,
The culture apparatus comprises:
A culture system comprising: an information output unit that outputs setting change information of environmental conditions of the temperature-controlled room as the prediction information to the acquisition unit. A culture system comprising the nitrogen supply device according to any one of claims 1 to 3 and a culture device for culturing a sample of a culture vessel in a thermostatic chamber adjusted to a predetermined environmental condition,
The culture apparatus comprises:
An evaluation unit for evaluating the environmental conditions of the temperature-controlled room;
A culture system comprising: an information output unit that outputs evaluation information from the evaluation unit to the acquisition unit as the prediction information. The culture system according to claim 4,
The nitrogen supply device includes:
A determination unit that determines whether or not the control unit can control the supply amount of the nitrogen gas;
An information output unit that outputs the determination information by the determination unit to the culture apparatus;
The culture apparatus comprises:
An acquisition unit for acquiring the determination information;
A culture system comprising: a control unit that controls whether the open / close unit can be opened or closed according to the determination information. In the culture system according to any one of claims 4 to 7,
The nitrogen supply device includes:
Installed separately from the culture apparatus,
The culture apparatus comprises:
It consists of a first housing and a second housing that is installed separately from the first housing, and comprises a microscope,
The microscope is
The first housing has a sample stage and the temperature-controlled room, and the second stage has a driving unit for the sample stage and a driving unit for an objective lens of a microscope for observing the sample placed on the sample stage. A culture system characterized by comprising:

Images