JP2009120521A - Cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic having a high safety and also having excellent improving effects in wrinkle inhibition and bleaching. <P>SOLUTION: This cosmetic includes methylated catechins as active ingredients. Thereby, it is possible to provide the cosmetic having the high safety and also having the excellent improving effects in the wrinkle inhibition and bleaching. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a cosmetic containing catechins, particularly methylated catechins as an active ingredient.

In recent years, improving wrinkles and whitening and maintaining beautiful skin has become a major concern for both young and old.
Wrinkles are thought to occur due to aging and skin aging. Although the mechanism of skin aging has not yet been clarified, adverse effects due to environmental factors, particularly ultraviolet rays, are considered. Active oxygen produced by ultraviolet rays degrades cell function and causes skin aging and wrinkles.

  On the other hand, whitening can also be adversely affected by ultraviolet rays. Ultraviolet rays oxidize tyrosine present in the skin by the action of the tyrosinase enzyme to produce a melanin pigment. If excessively produced, it causes skin problems such as spots, buckwheat, and darkness.

  For this reason, extracts of natural products such as herbal medicines have been used as cosmetics in order to suppress wrinkles or improve whitening. Apart from this, compounds such as ascorbic acid derivatives and glutathione are also used as cosmetics.

On the other hand, it has been reported that catechins have various physiological activities. For example, Patent Document 1 contains an 8-OHdG (8-hydroxydeoxyguanosine) production inhibitor composed of catechins and / or glycosides thereof, and suppresses 8-OHdG production in DNA by ultraviolet rays in cells. Cosmetics for preventing or improving spots and wrinkles are disclosed.
Patent Document 2 discloses (−) catechin, (+) catechin, resveratrol, a plant containing the same, and one or two or more types selected from the extract thereof. Disclosed is an inhibitor that is useful for preventing wrinkle formation and skin softness loss, preventing aging of vascular tissue with age, preventing aging of skin tissue, vascular tissue and lens with diabetes It is said that.
JP 2003-2819 A JP 2002-293736 A

  An object of the present invention is to provide a cosmetic that is highly safe and has an excellent effect of suppressing wrinkles and improving whitening.

  The present invention provides a cosmetic comprising methylated catechins as active ingredients.

  ADVANTAGE OF THE INVENTION According to this invention, the cosmetics which have high safety | security, and have the further excellent wrinkle suppression and whitening improvement effect can be provided.

Hereinafter, the present invention will be described in more detail.
The methylated catechin used in the present invention can be represented by the following general formula (1).

(In general formula (1), R ₁ , R ₂ , R ₃ and R ₄ each independently represents a hydrogen atom or a methyl group, and X ₁ and X ₂ each independently represent a hydrogen atom or a hydroxyl group)

  Among them, methylated catechins useful in the present invention are represented by the above general formula (1), and epigallocatechin-3-O- (3-O-methyl) gallate, epicatechin-3-O- (3-O -Methyl) gallate, epicatechin-3-O- (4-O-methyl) gallate, epigallocatechin-3-O- (4-O-methyl) gallate, gallocatechin-3-O- (3-O-methyl) ) Galate, catechin-3-O- (3-O-methyl) gallate, catechin-3-O- (4-O-methyl) gallate, gallocatechin-3-O- (4-O-methyl) gallate, and these Is an isomer.

  Methylated catechins are available in “Benifuuki”, “Benifuji”, “Benihorome”, “Yaeho”, “Surugasuse”, “Yutaka Midori”, “Kana Midori”, “Okumusashi”, “ `` Blue heart large bread '', `` Blue heart dragon '', `` Ooba dragon '', `` Safflower '', `` Benihikari '', `` Yamakai '', `` Yamamidori '', `` Karabeni '', `` Kou '', “Sofu” and “Okumidori”, or a mixture of these can be derived from tea leaves.

Methylated catechin can be easily extracted by drying, pulverizing, and immersing the tea leaves in warm water (for example, 10 ° C. to 100 ° C.).
The obtained extract may be purified by column chromatography.

The cosmetic of the present invention is more preferably in a form containing methylated catechin and coenzyme Q10. Coenzyme Q10 is known as ubidecarenone or coenzyme Q10 and is a lipophilic solid with a low melting point.
A commercially available dispersion of Coenzyme Q10 can also be used, for example, a dispersion manufactured by Nisshin Pharma.
Further, coenzyme Q10 is preferable because the reduced form suppresses oxidation of methylated catechins and increases the effect of suppressing wrinkles and enhancing whitening. The method for obtaining reduced coenzyme Q10 is not particularly limited. For example, after obtaining coenzyme Q10 by a conventionally known method such as synthesis, fermentation, extraction from natural products, etc., reduced coenzyme in the effluent is obtained by chromatography. A method of concentrating the Q10 section can be adopted. In this case, a general reducing agent such as sodium borohydride or sodium dithionite (hydrosulfite sodium) is added to the coenzyme Q10 as necessary, and the coenzyme Q10 is added to the coenzyme Q10 by a conventional method. The oxidized coenzyme Q10 contained may be reduced to form reduced coenzyme Q10, followed by concentration by chromatography. It can also be obtained by a method in which the above reducing agent is allowed to act on existing high-purity coenzyme Q10.

  The blending amount of the methylated catechin of the cosmetic of the present invention is preferably about 0.00001 to 30% by mass as a dry weight, but depending on various conditions such as a dosage form to be used, a target to be used, etc. The blending amount can be appropriately set in a wide range.

  The cosmetic of the present invention is usually used as an external preparation, but may be administered orally depending on the case.

  The cosmetics of the present invention include fats and oils such as vegetable oils, higher fatty acids, higher alcohols, silicones, anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, preservatives, sugars, metal ions A blocking agent, a polymer such as a water-soluble polymer, a thickener, a powder component, an ultraviolet absorber, an ultraviolet blocking agent, a moisturizing agent such as hyaluronic acid, a fragrance, a pH adjuster, and the like can be contained. In addition, other medicinal components such as vitamins, skin activators, blood circulation promoters, resident bacteria control agents, active oxygen scavengers, anti-inflammatory agents, bactericides, and physiologically active components can be contained.

  Examples of the fats and oils include camellia oil, evening primrose oil, macadamia nut oil, olive oil, rapeseed oil, corn oil, sesame oil, jojoba oil, germ oil, wheat germ oil, triglycerin, trioctanoic acid glycerin, etc. , Palm oil, hydrogenated coconut oil, palm oil, palm kernel oil, owl, owl kernel oil, hydrogenated oil, hardened castor oil and other solid fats, beeswax, candelilla wax, cotton wax, nuka wax, lanolin, lanolin acetate, liquid lanolin And waxes such as sugarcane wax, liquid paraffin, squalene, squalane, and microcrystalline wax.

  Examples of higher fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and the like.

  Examples of the higher alcohol include linear alcohols such as lauryl alcohol, stearyl alcohol, cetyl alcohol, and cetostearyl alcohol, and branched chain alcohols such as monostearyl glycerol ether, lanolin alcohol, cholesterol, phytosterol, and octyldodecanol.

  Examples of the silicone include linear polysiloxanes such as dimethylpolysiloxane and methylphenylpolysiloxane, and cyclic polysiloxanes such as decamethylpolysiloxane.

  Examples of the anionic surfactant include fatty acid salts such as sodium laurate, higher alkyl sulfates such as sodium lauryl sulfate, alkyl ether sulfates such as POE lauryl sulfate triethanolamine, N-acyl sarcosine acid, sulfosuccinate , N-acylamino acid salts and the like.

  Examples of the cationic surfactant include alkyltrimethylammonium salts such as stearyltrimethylammonium chloride, benzalkonium chloride, and benzethonium chloride.

  Examples of amphoteric surfactants include betaine surfactants such as alkyl betaines and amide betaines.

  Examples of nonionic surfactants include sorbitan fatty acid esters such as sorbitan monooleate and hydrogenated castor oil derivatives.

  Examples of preservatives include methyl paraben and ethyl paraben.

  Examples of the sequestering agent include edetates such as disodium ethylenediaminetetraacetate, edetic acid, and sodium edetate.

  Examples of polymers include gum arabic, gum tragacanth, galactan, guar gum, carrageenan, pectin, agar, quince seed, dextran, pullulan, carboxymethyl starch, collagen, casein, gelatin, methylcellulose, methylhydroxypropylcellulose, hydroxyethylcellulose, carboxymethylcellulose Examples thereof include vinyl polymers such as sodium (CMC), sodium alginate, carboxyvinyl polymer (such as CARBOPOL), and bentonite.

  Examples of the thickener include carrageenan, gum tragacanth, quince seed, casein, dextrin, gelatin, CMC, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxyvinyl polymer, guar gum, xanthan gum and the like.

  Examples of the powder component include talc, kaolin, mica, silica, zeolite, polyethylene powder, polystyrene powder, cellulose powder, inorganic white pigment, inorganic red pigment, titanium oxide coated mica, titanium oxide coated talc, and colored titanium oxide coated mica. And organic pigments such as Red No. 201 and Red No. 202.

  Examples of the ultraviolet absorber include paraaminobenzoic acid, phenyl salicylate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, and 2,4-dihydroxybenzophenone.

  Examples of the ultraviolet blocking agent include titanium oxide, talc, carmine, bentonite, kaolin, and zinc oxide.

  Examples of humectants include polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, diglycerin, polyglycerin, xylitol, maltitol, maltose, sorbitol, glucose, fructose, sucrose, lactose, and chondroitin. Examples thereof include sodium sulfate, sodium hyaluronate, sodium lactate, pyrrolidone carboxylic acid, cyclodextrin and the like.

  Examples of medicinal ingredients include vitamin A oil, vitamin A such as retinol, vitamin B2 such as riboflavin, B6 such as pyridoxine hydrochloride, L-ascorbic acid, L-ascorbic acid phosphate, L-ascorbic acid Vitamin C such as monopalmitic acid ester, L-ascorbic acid dipalmitic acid ester, L-ascorbic acid-2-glucoside, pantothenic acid such as calcium pantothenate, vitamin D such as vitamin D2, cholecalciferol; α- Vitamins such as vitamin E such as tocopherol, tocopherol acetate and DL-α-tocopherol nicotinate can be mentioned.

  In addition, whitening agents such as placenta extract, glutathione, yukinoshita extract, skin activators such as royal jelly, beech tree extract, blood circulation promotion such as capsaicin, gingeron, cantalis tincture, ictamol, caffeine, tannic acid, γ-oryzanol Agents, glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, anti-inflammatory agents such as azulene, amino acids such as arginine, serine, leucine and tryptophan, maltose sucrose condensates of resident bacteria control agents, lysozyme chloride and the like.

  In addition, chamomile extract, parsley extract, wine yeast extract, grapefruit extract, honeysuckle extract, rice extract, grape extract, hop extract, rice bran extract, loquat extract, buckwheat extract, yakuinin extract, assembly extract, merirot extract, birch extract, elephant extract, peony extract, Soap extract, loofah extract, capsicum extract, lemon extract, gentian extract, perilla extract, aloe extract, rosemary extract, sage extract, thyme extract, tea extract, seaweed extract, cucumber extract, carrot extract, carrot extract, maronier extract, hamamelis You may mix | blend various extracts, such as an extract and a mulberry extract.

  The cosmetics of the present invention are, for example, skin cosmetics such as lotions, emulsions, creams, packs, makeup base lotions, makeup creams, emulsions, cream-like or ointment-type foundations, lipsticks, eye colors, cheek colors, etc. It can be used as a bath preparation such as makeup cosmetics, hand creams, leg creams, body cosmetics such as body lotions.

  As a compounding quantity of the methylated catechin of the cosmetics of this invention, although 0.00001-30 mass% is preferable as a dry weight, it is not necessarily restrict | limited to this.

  Since the methylated catechin of the present invention is a natural product, its toxicity is low, and it is safe and excellent in price.

  EXAMPLES Hereinafter, although an Example and a comparative example further demonstrate this invention, this invention is not restrict | limited to the following example.

Example 1
Extraction was obtained from Benifuuki tea leaves at 90 ° C. using 30 parts by mass of pure water.
Melanin suppression activity was measured by the following test using the said extract.

As cells producing melanin, cultured B16 melanoma cells derived from mice were cultured in Eagle's MEM medium supplemented with fetal bovine serum to a final concentration of 10%, and the cells were cultured at a concentration of 3 × 10 ³ cells / ml. Inoculate 6 ml of each well of a 6-well plate, and after culturing in a CO ₂ incubator for 5 days, replace the medium with the test sample added, and further culture under the same conditions for 3 days. After washing the cells, the cells are peeled off by a scraper treatment, solubilized with sodium dodecyl sulfate (SDS), and the absorbance at 475 nm and 260 nm is measured to obtain S ₄₇₅ and S ₂₆₀ . The melanin inhibition rate was calculated by Equation 1 with the absorbance at 475 nm and 260 nm of cells cultured in a medium not added with the test sample as C ₄₇₅ and C ₂₆₀ . As a positive control, kojic acid was used. The results are shown in Table 1.

  From Table 1, it became clear that methylated catechin has a whitening effect.

Comparative Example 1
In Example 1, Example 1 was repeated except that epicatechin gallate was used instead of the extract. The concentration of epicatechin gallate is 500 μg / ml as in Example 1.
As a result, the melanin suppression rate was 23.2%.

Comparative Example 2
In Example 1, Example 1 was repeated except that epigallocatechin gallate was used instead of the extract. The epigallocatechin gallate concentration is 500 μg / ml as in Example 1.
As a result, the melanin suppression rate was 20.9%.

Example 2
In Example 1, in addition to methylated catechin, Example 1 was repeated except that Nisshin Pharma Coenzyme Q10 emulsifier (10% solution) was further used. The addition concentration of coenzyme Q10 is 500 μg / ml, similar to methylated catechin.
As a result, the melanin suppression rate was 60.1%.

Example 3
Lotions were prepared as cosmetics as shown in the table below.

Example 4
As shown in the table below, creams were prepared as cosmetics.

Example 5
Extraction was obtained by adjusting the concentration of methylated catechins by performing extraction at 90 ° C. using 30 parts by mass of pure water with respect to Benifuuki tea leaves.
A wrinkle formation suppression test was performed by the following test using the above extract.

Wrinkle formation inhibition test using hairless mice: The above extract (300 μg / ml as methylated catechin) or ethanol as a control was applied to 80 μl on the back of 6 hairless mice (HR / ICR, 6 weeks old at the start of the experiment). . After 2 hours, the extract on the skin surface was wiped off with an ethanol solution and irradiated with UV-B light. This operation was performed 5 times a week for 10 weeks. Irradiation is measured with a UV-Radiometer (manufactured by TOKYO OPTICAL, UVR-305 / 365D), and the total amount of irradiation is 0.28 mW / cm ² so that one dose is 1 MED or less. The amount was 100 mJ / cm ² . After completion of the test, the degree of wrinkle formed was divided into the following four stages, a standard photo was set for each stage, and the degree of wrinkle of each mouse was evaluated as a wrinkle index while comparing with the standard picture. .
Wrinkle index 1: Wrinkle is faintly formed 2: Wrinkle is lowly formed 3: Wrinkle is moderately formed 4: Wrinkle is highly formed As a result, the wrinkle index is 1.6 ± 0.2 in the present invention group. The control was 3.5 ± 0.3.

Claims (1)

  Cosmetics containing methylated catechin as an active ingredient.