JP2009013160A - Succinoyl trehalose lipid composition, solution and emulsion composition thereof, and method for producing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a solid STL (succinoyl trehalose lipid) composition which is white, readily soluble in an aqueous solvent and capable of affording a colorless transparent aqueous solution. <P>SOLUTION: The solid STL composition can be obtained by culturing a microorganism in a culture medium containing a carbon source and purifying the culture medium according to an original method for purification. The composition is white, readily soluble in the aqueous solvent and capable of affording the colorless transparent aqueous solution and forming a stable emulsion with an aqueous ingredient and an oily ingredient. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a biosurfactant, and more particularly, to a succinoyl trehalose lipid (STL) composition.

  A biosurfactant, which is a functional substance produced by a microorganism, is an amphiphilic substance having a hydrophobic part and a hydrophilic part. In particular, glycolipid-type biosurfactants are attracting attention as natural surfactants exhibiting excellent biodegradability, and are also used as additives for cosmetics, foods, etc. because they exhibit a higher moisturizing effect and emulsifying action. It is known to obtain (Non-Patent Document 1).

  One of the glycolipid type biosurfactants is succinoyl trehalose lipid. The succinoyl trehalose lipid is obtained by the following method.

  In Patent Document 1 and Non-Patent Document 2, Rhodococcus erythropolis strain SD-74 is aerobically cultured in a medium containing fatty acid or vegetable oil to produce a succinoyl trehalose lipid mixture. ing. Further, in Patent Document 2, Rhodococcus sp. Strain TB-42 is aerobically cultured in a medium containing unsaturated hydrocarbons, halogen hydrocarbons, etc., so that a succinoyl trehalose lipid mixture is obtained. It has produced.

  Thus, since succinoyl trehalose lipid is obtained by culturing microorganisms in a medium containing a carbon source, it is produced as a mixture in which impurities are mixed. When used in cosmetics, foods, etc., it is preferable to purify the above mixture. In particular, it is preferable to perform dehydration and deoiling to obtain a solid succinoyl trehalose lipid composition from the viewpoint of storage, transportation, use, and the like.

For example, in Non-Patent Document 3, succinoyl trehalose lipid is precipitated by removing cells, residual substrates, and the like from a culture solution containing a succinoyl trehalose lipid mixture and then acidifying the culture solution. The acid precipitate is washed twice with water and then dissolved in methanol. The solution is washed three times with hexane, and the methanol is distilled off to recover the solid succinoyl trehalose lipid composition. .
JP 62-83896 (April 17, 1987) JP 10-72478 A (published March 17, 1998) Y. Ishigami et al. Jpn. Oil Chem. Soc. 36 (11): 847-851 (1987). Y. Uchida et al., Agric. Biol. Chem. 53 (3): 765-769 (1989) Y. Uchida et al., Agric. Biol. Chem. 53 (3): 757-763 (1989).

  However, the solid succinoyl trehalose lipid composition obtained by the conventional method was light brown. When the colored succinoyl trehalose lipid composition is used as an additive for cosmetics, foods, etc., these products are unnecessarily colored, which is not preferable.

  Further, as a result of investigations by the present inventors, it has been found that the solid succinoyl trehalose lipid composition obtained by the conventional method is hardly dissolved in an aqueous solvent, and the resulting solution is also cloudy. That is, the conventional solid succinoyl trehalose lipid composition has been difficult to use as an aqueous solution. Therefore, it is also difficult to obtain an emulsified composition by a technique such as mechanical stirring.

  As described above, the solid succinoyl trehalose lipid composition obtained by the conventional method is (1) colored, (2) hardly dissolved in an aqueous solvent, and (3) a solution containing the composition. Had the problem of turbidity.

  The present invention has been made in view of the above problems, and an object thereof is to provide a high-quality succinoyl trehalose lipid composition in which at least one of the above problems is solved.

  The present inventors have found that the above problems have been solved in a solid succinoyl trehalose lipid composition obtained by a unique purification method, and have completed the invention.

  That is, the solid succinoyl trehalose lipid composition according to the present invention is characterized by any one or more of the following (A) to (E): (A) culturing a microorganism in a medium containing a carbon source. Obtained and can be dissolved in water by at least 1% by mass; (B) obtained by culturing a microorganism in a medium containing a carbon source and white; (C) of a solution when dissolved by 5% by mass in methanol. Absorbance is 0.05 or less over the wavelength range of 400 nm to 700 nm; (D) at least 5% by weight dissolved in water; or (E) light at a wavelength of 660 nm of an aqueous solution when dissolved at 1% by weight in water. The transmittance is 95% or more.

  The solid succinoyl trehalose lipid composition according to the present invention has the characteristics as described above, and is at least white, easily dissolved in an aqueous solvent, or dissolved in an aqueous solvent. A clear solution. Therefore, it can be handled as an aqueous solution, and even when used as an additive for an arbitrary product, the product is not unnecessarily colored, and thus can be suitably used for various applications.

  The succinoyl trehalose lipid solution according to the present invention is characterized in that the solid succinoyl trehalose lipid composition according to the present invention is dissolved in a solvent. The solvent may be an aqueous solvent.

  The succinoyl trehalose lipid solution according to the present invention may also be one in which succinoyl trehalose lipid obtained by culturing a microorganism in a medium containing a carbon source is dissolved in an aqueous solvent in an amount of 1% by mass or more.

  The succinoyl trehalose lipid solution may be one in which the succinoyl trehalose lipid is dissolved in the aqueous solvent in an amount of 5% by mass or more.

  The succinoyl trehalose lipid solution may have a light transmittance of 95% or more at a wavelength of 660 nm.

  As described above, the succinoyl trehalose lipid solution according to the present invention is transparent because the succinoyl trehalose lipid is dissolved in an aqueous solvent. Therefore, even if it is an aqueous solution and used as an additive for an arbitrary product, the product is not unnecessarily colored, and thus can be suitably used for various applications.

  The succinoyl trehalose lipid solution preferably further contains alkali metal ions or alkaline earth metal ions.

  According to said structure, a succinoyl trehalose lipid can be suitably dissolved in an aqueous solvent.

  The emulsified composition according to the present invention includes the solid succinoyl trehalose lipid according to the present invention, an aqueous component, and an oil component.

  According to said structure, the milky white stable emulsion composition can be provided.

  The method for producing an emulsified composition according to the present invention comprises preparing a succinoyl trehalose lipid aqueous solution by dissolving the solid succinoyl trehalose lipid according to the present invention in an aqueous solvent, and the succinoyl trehalose lipid aqueous solution, It includes the step of emulsifying the ingredients.

  According to said structure, since the solid succinoyl trehalose lipid based on this invention can be easily melt | dissolved in an aqueous solvent, an emulsion composition can be manufactured easily.

  The solid succinoyl trehalose lipid composition according to the present invention is at least white, easily dissolved in an aqueous solvent, or becomes a transparent solution when dissolved in an aqueous solvent. Therefore, it can be handled as an aqueous solution or an emulsified composition, and even when it is used as an additive for an arbitrary product, the product is not undesirably colored, and thus can be suitably used for various applications.

[1: Solid succinoyl trehalose lipid composition]
The present invention provides a solid succinoyl trehalose lipid composition. As used herein, the term “composition” is intended to mean a form in which various components are contained in one substance, and “succinoyl trehalose lipid composition” means succinoyl trehalose lipid as one component. Is intended as a composition. In one embodiment, the succinoyl trehalose lipid may be contained in the succinoyl trehalose lipid composition in an amount of 50% by mass or more, preferably 80% by mass or more, and preferably 90% by mass or more. Is more preferable. In addition, the term “solid” is intended to indicate the properties of a substance having a certain shape including powder, granules, etc., and “solid succinoyl trehalose lipid composition” means succinoyl trehalose lipid. Are intended to have a certain shape.

  In one embodiment, the solid succinoyl trehalose lipid composition according to the present invention is obtained by culturing a microorganism in a medium containing a carbon source.

  As used herein, “microorganism” is intended to be a microorganism capable of producing succinoyl trehalose lipids. Such a microorganism is not particularly limited, but is preferably a microorganism belonging to the genus Rhodococcus, and is preferably Rhodococcus erythropolis SD-74 strain, Rhodococcus sp. TB-42 strain, or Rhodococcus baikonurensis NBRC 100611 strain. preferable.

  As used herein, the “carbon source” is a carbon compound that is absorbed and utilized by the microorganism during culture, and is preferably a natural fat, hydrocarbon, fatty acid, fatty acid ester, or higher alcohol. . Here, the carbon compound is intended to be a compound of carbon and hydrogen, nitrogen or the like. In one embodiment, the carbon source used in the production method according to the present invention may be natural fats and oils, may be animal fats or vegetable oils, but is vegetable oils and fats because it is easier to obtain. It is preferable. The vegetable oil used in the above production method is preferably, but not limited to, palm oil, coconut oil, soybean oil, olive oil, safflower oil, rapeseed oil, corn oil, cottonseed oil, tall oil, and the like.

  The hydrocarbon used as the carbon source includes normal alkanes such as n-decane, n-undecane, n-tridecane, n-tetradecane, n-pentadecane, n-hexadecane, n-heptadecane, n-octadecane, n-nonadecane, etc. 1-decene, 1-undecene, 1-dodecene, 1-tridecene, 1-tetradecene, 1-pentadecene, 1-hexadecene, 1-heptadecene, normal alkene such as 1-octadecene, and the like can be suitably used. It is.

  Fatty acids used as carbon sources include decanoic acid, undecanoic acid, dodecanoic acid (lauric acid), tridecanoic acid, tetradecanoic acid (myristic acid), pentadecanoic acid, hexadecanoic acid (palmitic acid), heptadecanoic acid, octadecanoic acid (stearic acid) It is possible to suitably use oleic acid or the like. Further, higher alcohols such as fatty acid esters, undecyl alcohol, and dodecyl alcohol (lauryl alcohol) may be used as the carbon source.

  In one aspect, a succinoyl trehalose lipid obtained by culturing a microorganism in a medium containing a carbon source has a sugar moiety of trehalose, and succinic acid and fatty acid are each linked by 1 to 2 moles of ester per mole of trehalose. It is a lipid. Different fatty acids are bound to the fatty acid portion of the glycolipid by changing the carbon source as a culture substrate.

  In one embodiment, the solid succinoyl trehalose lipid composition according to the present invention may be white. In one aspect, the solution in which 5% by mass of the solid succinoyl trehalose lipid composition according to the present invention is dissolved in methanol has an absorbance exceeding 0.15 over the visible light region (wavelength range of 400 nm to 700 nm). And preferably does not exceed 0.1 and more preferably does not exceed 0.05. In addition, the said light absorbency is the value measured with the spectrophotometer (UV-2550, Shimadzu Corporation).

  On the other hand, the solid succinoyl trehalose lipid composition according to the prior art is light brown (see Non-Patent Document 3) and not white. A solution in which 5% by mass of a solid succinoyl trehalose lipid composition according to the prior art is dissolved in methanol has an absorbance exceeding 0.05 in a wavelength range of 400 nm to 550 nm, and an absorbance in a wavelength range of 400 nm to 450 nm. Absorbance exceeded 0.15 in the region where the wavelength exceeded 0.1 and the wavelength ranged from 400 nm to 420 nm.

  Since the solid succinoyl trehalose lipid composition according to the present embodiment is white, it can be suitably used for various applications. For example, when the composition according to the present embodiment is used as an additive for foods, cosmetics and the like, foods, cosmetics and the like are not unnecessarily colored, and therefore can be suitably used.

  In one embodiment, the solid succinoyl trehalose lipid composition according to the present invention has good solubility in an aqueous solvent. In one aspect, the composition can be dissolved at least 1% by weight homogeneously in water, preferably 3%, 5%, 7%, or 7.5% by weight homogeneously dissolved. On the other hand, the solid succinoyl trehalose lipid composition according to the prior art does not dissolve 1% by mass or more in water, as will be described later. Since the solid succinoyl trehalose lipid composition according to the present embodiment is homogeneously dissolved in water, it can be handled as an aqueous solution and can be suitably used in foods or cosmetics based on water. The application is not limited to this, and any application that can be used as an aqueous solution can be used.

  Furthermore, in one embodiment, the solid succinoyl trehalose lipid composition according to the present invention becomes a colorless and transparent solution when dissolved in water, as described later. If the colorless and transparent is represented by the light transmittance at a wavelength of 660 nm, which is generally used as an index indicating the turbidity of an aqueous solution, the light transmittance can be 95% or more, preferably 97% or more, more preferably May be 99% or more. The light transmittance is obtained by dissolving 1% by mass of the succinoyl trehalose lipid composition in water having a purity of 95% or more and measuring the light transmittance at a wavelength of 660 nm using the spectrophotometer. It is done.

  As shown in the Example mentioned later, in one Embodiment, the light transmittance in wavelength 660nm of the aqueous solution when 1 mass% of solid succinoyl trehalose lipid composition concerning this invention is dissolved in water by pH 7.0 is shown. Is 99.5%. On the other hand, the solid succinoyl trehalose lipid composition according to the prior art has a light transmittance of 4.8% at a wavelength of 660 nm of an aqueous solution mixed with 1% by mass of water, as shown in Examples described later. . As described above, the solid succinoyl trehalose lipid composition according to the present invention can form a highly transparent solution compared to the solid succinoyl trehalose lipid composition according to the prior art.

  As described above, when the solid succinoyl trehalose lipid composition according to the present embodiment is used, a colorless and transparent succinoyl trehalose lipid solution as described later can be prepared.

  In one embodiment, the solid succinoyl trehalose lipid composition according to the present invention includes a step of culturing a microorganism in a medium containing a carbon source, a precipitation step of precipitating a product obtained by the culture step, and It can be produced by a production method including an extraction step for extracting the STL composition from the precipitate obtained by the precipitation step and a fat-soluble substance removal step for removing the fat-soluble substance from the extract obtained by the extraction step. Hereinafter, the production method will be described in detail.

(Culture process)
The step of culturing microorganisms in a medium containing a carbon source is performed according to a conventional method, and nutrients such as a nitrogen source and inorganic salts may be added to the medium to which the carbon source has been added, as necessary. As the carbon source added to the medium, each of the carbon sources described above is preferably used, and the concentration of the carbon source in the medium is preferably 5 to 20% by mass, and more preferably 10% by mass. preferable. As the nitrogen source added to the medium, organic or inorganic substances containing nitrogen that are usually used for culturing microorganisms are used. For example, sodium nitrate, potassium nitrate, potassium hydrogen phosphate, potassium dihydrogen phosphate, etc. can be used. is there. In addition to the above, nutrients such as yeast extract and peptone may be added to the medium if necessary for the growth of microorganisms.

  The culture is performed under aerobic conditions by shaking and stirring, and the culture temperature is preferably 20 to 35 ° C, more preferably 30 ° C. The culture pH is preferably 5.5 to 9.5. In addition, the culture period is preferably cultured until a succinoyl trehalose lipid mixture having a concentration of 15 to 50 g / l is produced. In the examples described later, the succinoyl trehalose lipid concentration is 15 g / l after 5 days of culture. Therefore, it is preferable to culture for 5 to 12 days.

  In the step of culturing a microorganism in a medium containing a carbon source, seed culture may be performed before the microorganism is main-cultured. By seed-culturing the microorganism, it is possible to adjust the microorganism to optimum conditions, and as a result, succinoyl trehalose lipid can be produced efficiently.

(Precipitation process)
Next, the product (STL) produced by the microorganism in the culture step is deposited (precipitation step). That is, precipitation is performed on a medium or culture solution containing STL produced by microorganisms in the medium. At this time, the culture medium or the culture solution to be deposited may be obtained by centrifuging the culture solution in which the microorganism is cultured and removing the cells and the remaining substrate from the culture solution. Here, “precipitating” is intended to take out a substance dissolved in a medium as a solid. That is, in the precipitation step according to the present invention, the glycolipid produced in the medium by the microorganism in the culture step can be taken out from the medium as a solid.

  The precipitation step is not particularly limited as long as it is a method capable of separating STL as a solid from the culture solution, and a conventional method can be used. For example, STL, which is an acidic glycolipid, can be precipitated by acidifying the medium in which the microorganisms are cultured in the culturing step and precipitating an acidic substance in the medium (acid precipitation). Specifically, it is precipitated by lowering the pH of the culture solution (object). In order to lower the pH of the culture solution, an acidic substance such as HCl may be added. Thereafter, for example, a centrifugal treatment is performed to take out the precipitate. Hereinafter, the product obtained through the precipitation step is referred to as “deposition product”.

(Extraction process)
Next, an STL composition is extracted from the precipitated product (extraction process). In the extraction step, the STL contained in the precipitation product is dissolved in the solvent layer by adding a solvent that is incompatible with water and soluble in glycolipid to the precipitation product. Subsequently, the STL composition from which the water-soluble substance has been removed can be obtained by separating the solvent layer in which the STL is dissolved. The “water-soluble substance” is a substance that is soluble in water, and can be said to be a water-soluble impurity such as a salt contained in the solid precipitate precipitated from the medium together with water. . Here, the “salt” is a compound in which a hydrogen atom of an acid is replaced with a metal or other metallic group.

  The above-described precipitation product precipitated from the culture medium in which the microorganism is cultured contains water and contains a large amount of water-soluble impurities. Such a precipitation product further contains fat-soluble impurities and is in the form of a solid, so even if it is washed with water, it is difficult to sufficiently remove the water-soluble substance contained in the precipitate. However, an STL composition from which water-soluble substances have been removed can be obtained by an extraction process using a solvent that is incompatible with water and in which the glycolipid is soluble.

  In the extraction process, it is not necessary to completely remove the water-soluble substance from the precipitate, which is an object to be extracted by adding a solvent, and the amount of the water-soluble substance in the precipitate is reduced before and after the extraction process. That's fine. Thereby, it is possible to obtain an STL composition having a reduced impurity content.

  Here, as the solvent to be added to the precipitate, a solvent that is incompatible with another solvent (for example, water) that can dissolve the water-soluble substance and is soluble in glycolipid can be used. Examples of such solvents include ester solvents, alcohol solvents, and hydrocarbon solvents, and specific examples include ethyl acetate, 1-butanol, and xylene. The amount of the solvent added to the precipitate is preferably 0.1 to 10 times the mass of the precipitate, and more preferably the same as the amount of the precipitate.

  The extraction process will be described with reference to the case where ethyl acetate is used as a solvent. First, a culture product is precipitated from the culture solution, ethyl acetate is added to the deposited precipitate, and the mixture is sufficiently stirred and separated into two layers, an ethyl acetate layer and an aqueous layer. Next, the ethyl acetate layer formed in the upper layer is separated by a separatory funnel or the like. Since the water-soluble substance is not dissolved in the ethyl acetate layer and the STL is dissolved, it is possible to remove the water-soluble substance from the glycolipid by separating the ethyl acetate layer. Subsequently, the solid STL composition from which the water-soluble substance is removed can be obtained by removing ethyl acetate from the ethyl acetate layer using, for example, an evaporator.

  In the above description, the case where the precipitation process and the extraction process are sequentially performed has been described, but the present invention is not necessarily limited thereto. For example, an STL composition from which a water-soluble substance has been removed is obtained by adding a solvent to a medium or culture solution in which the product is dissolved without precipitating the culture product from the medium or culture solution and separating the solvent layer. It is also possible. At this time, what remove | excluded the microbial cell and the residual substrate from the culture solution which culture | cultivated microorganisms by centrifugation etc. is good also as a target which removes a water-soluble substance using a solvent. That is, in the extraction step, a substance that is a target for removing a water-soluble substance using a solvent is a medium or culture solution containing STL produced by a microorganism in culture in the medium, or a solid state separated from a reaction system. STL mixtures of

  The product obtained through this extraction step is referred to as “extraction product”.

(Fat-soluble substance removal process)
Next, the fat-soluble substance is removed from the dehydrated product (fat-soluble substance removing step). Here, the “fat-soluble substance” is intended to be a substance that is soluble in fats and oils.

  The method for removing the fat-soluble substance from the solid STL composition from which the water-soluble substance has been removed is not particularly limited as long as it is a method capable of separating the STL and the fat-soluble substance, and a conventional method is used. Can be used. For example, the solvent is first distilled off from the extracted product, and then a solvent capable of separating the glycolipid and the fat-soluble substance is added to the solid obtained by distilling off the solvent, and the solvent layer is added. By removing, a glycolipid from which the fat-soluble substance has been removed can be obtained. Thereby, fat-soluble impurities can be efficiently removed from the glycolipid.

  Here, the solvent capable of separating the glycolipid and the fat-soluble substance is a solvent in which the glycolipid is hardly soluble or insoluble and the fat-soluble substance is soluble. For example, when hexane is used as such a solvent, water-soluble substances are removed from the product precipitated from the culture solution using the solvent, and then the solid obtained by distilling off the solvent is suspended in hexane. Filter, or centrifuge to remove hexane. Thereby, fat-soluble impurities can be efficiently removed from the STL composition.

[2. Succinoyl trehalose lipid solution)
The present invention also provides a succinoyl trehalose lipid solution. The term “succinoyl trehalose lipid solution” intends a solution comprising succinoyl trehalose lipid.

  In one embodiment, the succinoyl trehalose lipid solution according to the present invention is obtained by dissolving the solid succinoyl trehalose lipid composition according to the present invention in a solvent. The solvent may be any solvent that can dissolve the succinoyl trehalose lipid, and as such a solvent, as described above, an aqueous solvent or other solvent (such as an organic solvent) can be used. A person skilled in the art can appropriately select a solvent according to the use of the solution.

  In the present specification, the term “aqueous solvent” is intended to be a solvent mainly composed of water or a hydrophilic solvent. That is, as the component of the aqueous solvent, a substance that is compatible (miscible) with water can be used in addition to water. In particular, the aqueous solvent preferably contains no hydrophobic liquid. As a component of such an aqueous solvent, water, a C1-C4 lower alcohol, a polyhydric alcohol, a mixed solvent thereof, or the like can be preferably used.

  Examples of the lower alcohol having 1 to 4 carbon atoms include methanol, ethanol, propanol, isopropanol, and butanol. Polyhydric alcohols include propylene glycol, dipropylene glycol, glycerin, polypropylene glycol, diglycerin, polyglycerin, ethylene glycol, diethylene glycol, polyethylene glycol, 1,3-propanediol, 1,4-propanediol, 1, An example is 3-butanediol.

  In one embodiment, the succinoyl trehalose lipid solution according to the present invention comprises 1% by mass or more of succinoyl trehalose lipid obtained by culturing a microorganism in a medium containing a carbon source, and is preferably dissolved in an aqueous solvent. Is prepared by dissolving succinoyl trehalose lipid in an aqueous solvent in an amount of 3% by mass, 5% by mass, or 7% by mass or more. As will be described later, the solid succinoyl trehalose lipid composition according to the prior art cannot be dissolved in an aqueous solvent of 1% by mass or more, and thus conventionally obtained by culturing microorganisms in a medium containing a carbon source. There was no solution in which succinoyl trehalose lipid was dissolved in an aqueous solvent of 1% by mass or more. The succinoyl trehalose lipid solution according to this embodiment can be suitably used for storage, transportation, use, and the like because the succinoyl trehalose lipid is dissolved in an aqueous solvent at a high concentration.

  In one aspect, the succinoyl trehalose lipid solution according to this embodiment is colorless and transparent. If the colorless and transparent is represented by the light transmittance at a wavelength of 660 nm, which is generally used as an index indicating the turbidity of an aqueous solution, the light transmittance can be 95% or more, preferably 97% or more, more preferably May be 99% or more. The light transmittance is obtained by measuring the light transmittance at a wavelength of 660 nm using the spectrophotometer. As described above, since the solid succinoyl trehalose lipid composition according to the prior art makes the dissolved solution cloudy, conventionally, in the solution in which succinoyl trehalose lipid is dissolved in an aqueous solvent of 1% by mass or more, the wavelength There was no solution having a light transmittance at 660 nm of 5% or more. Since the succinoyl trehalose lipid solution according to the present embodiment is a colorless and transparent aqueous solution, for example, when used as an additive for foods, cosmetics, etc., it is preferable because it does not unnecessarily color foods, cosmetics, etc.

(Preparation method)
A method for preparing a succinoyl trehalose lipid solution according to the present invention will be described below. In one embodiment, the succinoyl trehalose lipid solution according to the present invention is prepared by dissolving a solid succinoyl trehalose lipid composition obtained by culturing a microorganism in a medium containing a carbon source in a solvent. When the solvent is an organic solvent, a succinoyl trehalose lipid solution can be easily prepared using a conventional method.

  When the solvent is an aqueous solvent, for example, a succinoyl trehalose lipid solution can be prepared by the following method.

  First, the solid succinoyl trehalose lipid composition and an aqueous solvent are mixed to obtain a mixed solution. At this time, since the succinoyl trehalose lipid which is the main component of the solid succinoyl trehalose lipid composition is an acidic substance having a carboxyl group in the molecular structure, the mixed solution is usually acidic.

  Here, in order to sufficiently dissolve the succinoyl trehalose lipid composition, it is preferable to neutralize the mixed solution. Neutralization of the mixed solution can be performed, for example, by adding an alkaline substance such as a sodium hydroxide solution to the mixed solution and further including alkali metal ions or alkaline earth metal ions in the mixed solution.

  As alkali metal ions, sodium ions, potassium ions, lithium ions and the like can be used. As alkaline earth metal ions, magnesium ions, calcium ions, beryllium ions and the like can be used, but not limited thereto. Any ion that can appropriately adjust the pH of the solvent may be used.

  Further, when the solid succinoyl trehalose lipid composition having a high concentration is dissolved in an aqueous solvent, it is preferable to ultrasonically treat the mixed solution in order to completely dissolve the composition.

[3: Emulsified composition]
The present invention further provides an emulsified composition. The term “emulsified composition” intends a composition obtained by emulsification and is sometimes referred to as an emulsion.

  In one embodiment, the emulsified composition according to the present invention contains the solid succinoyl trehalose lipid of the present invention, an aqueous component, and an oily component. The emulsified composition according to this embodiment is milky white and stable over a long period of time. Moreover, even when the content of succinoyl trehalose lipid, which is an emulsifying component, is low, it can be favorably emulsified, so that a large amount of an emulsified composition can be produced at low cost.

  As said aqueous component, the aqueous solvent used for the succinoyl trehalose lipid solution based on this invention can be used conveniently.

  The oil component is preferably liquid or pasty at 1 atm. 25 ° C., and particularly preferably liquid oil.

  Examples of such liquid oils include hydrocarbons, higher alcohol esters, higher fatty acid esters, triglycerides, silicone oils, higher alcohols, higher fatty acids, animal and vegetable oils, cholesterol fatty acid esters, and sterols. Sterol esters, polyphenols, and preferred are mineral oil, liquid paraffin, squalane, isopropyl palmitate, isopropyl myristate, isooctyl myristate, isotridecyl myristate, octadecyl myristate, octyldodecyl myristate, Stearyl cholesteryl ester, 2-ethylhexanoic acid triglyceride, cetyl 2-ethylhexanoate, sunflower oil, olive oil, jojoba oil, camellia oil, grape seed oil, avocado oil Dimethyl silicone oils such as macadamia nut oil, almond oil, rice germ oil, clove oil, orange oil, spruce oil, octamethyltrisiloxane, decamethyltetrasiloxane, methylpolysiloxane, highly polymerized methylpolysiloxane; octamethylcyclotetrasiloxane , Cyclic silicone oils such as decamethylcyclopentasiloxane, dodecamethylcyclohexacyclosan, methylcyclopolysiloxane, and methylpolycyclosiloxane; polyether-modified silicone oils, methylphenyl silicone oils such as methylphenylpolysiloxane, and the like. These may be used alone or in combination of two or more.

  In the emulsified composition according to the present invention, the content of STL is preferably 0.01% by mass to 5% by mass, and more preferably 0.05% by mass to 1% by mass. When the STL content is less than 0.01% by mass relative to the emulsified composition, there is a problem that emulsification may be insufficient. There is a problem that it is more economical.

  In the emulsified composition according to the present invention, the aqueous component: oil component is preferably 95: 5 to 30:70, and the mass ratio of the aqueous component and the oil component contained is 95: 5 to 50: More preferably, it is 50. When the mass ratio is higher than 30:70, the oil may not be completely emulsified.

  The solid STL used in the emulsified composition according to the present invention may be an STL salt. In the present specification, the STL salt means that one or both of the carboxyl groups of the succinic acid carboxyl groups bonded to one or two molecules of the STL obtained by the above-described production method have a hydrogen atom. Refers to those replaced by other metal cations or metallic groups.

  The STL salt contained in the emulsified composition according to the present invention is preferably a salt of STL and an alkali metal or alkaline earth metal. In this case, STL can be dissolved well in an aqueous solvent.

  Examples of the alkali metal constituting the salt with STL include sodium, potassium, lithium and the like, and examples of the alkaline earth metal include calcium, magnesium, beryllium and the like. Sodium or potassium is preferable, and sodium is particularly preferable. The sodium salt of STL is highly water-soluble and has the advantage that the solubility in a solvent is further improved.

  The salt of STL can be obtained by neutralizing STL with an alkaline compound containing an alkali metal or alkaline earth metal such as sodium hydroxide. Specifically, an STL salt can be obtained by the following method. First, STL is mixed with water so as to be a 1% by mass to 5% by mass aqueous solution, and an arbitrary alkaline compound is added while stirring with a stirrer to neutralize the aqueous solution, thereby dissolving the STL. At this time, the amount of the alkaline compound added is controlled so that the final pH becomes 7.0 to 7.2. Subsequently, the resulting aqueous solution is freeze-dried to obtain an STL salt. In addition, in the manufacturing method of the emulsion composition which concerns on this invention mentioned later, the solid STL salt obtained as mentioned above may be used, and the STL solution (neutralized with alkali before freeze-drying ( An aqueous solution in which a salt of STL is dissolved may be used.

(Production method)
Next, the manufacturing method of the emulsion composition concerning this invention is demonstrated. The production method of the emulsion composition is not particularly limited as long as the intended emulsion composition can be obtained, and various methods can be adopted.

  In one embodiment, the method for producing an emulsified composition according to the present invention comprises preparing a succinoyl trehalose lipid aqueous solution by dissolving the solid succinoyl trehalose lipid according to the present invention in an aqueous solvent, and the succinoyl trehalose. A step of emulsifying the aqueous lipid solution and the oily component is included. As described above, since the solid succinoyl trehalose lipid according to the present invention can be easily dissolved in an aqueous solvent, the emulsion composition according to the present embodiment can be easily manufactured. can do.

  The succinoyl trehalose lipid aqueous solution can be prepared as described above by dissolving the STL according to the present invention in an aqueous solvent. The STL dissolved in the aqueous solvent may be a salt of STL.

  For emulsification of the succinoyl trehalose lipid aqueous solution and the oil component, for example, the oil component is added to the succinoyl trehalose aqueous solution so that the mass ratio is as described above, and a well-known conventional technique such as a homogenizer is used. And stirring.

  In addition, when the succinoyl trehalose lipid aqueous solution is prepared by dissolving a salt of STL in an aqueous solvent, an aqueous gel composition is prepared from the succinoyl trehalose lipid aqueous solution, and then the aqueous gel-like composition is prepared. The above emulsification can also be carried out by adding an oil component to the composition and stirring the composition.

  Preparation of the aqueous gel composition from the succinoyl trehalose lipid aqueous solution can be performed by the following procedure. First, the solution containing the STL salt is acidified. This treatment can be performed, for example, by adding an acidic substance such as hydrochloric acid to a solution containing an STL salt. The solution containing the STL salt is neutral, and an acidic substance is added to the solution to make the solution acidic, preferably pH 6.0 to 6.9. Thereby, the solution containing the salt of STL can be easily gelled.

  In addition, in order to stabilize the state of a solution, it is preferable to leave a solution for a fixed time. Further, in the succinoyl trehalose lipid solution for preparing an aqueous gel-like composition, it is preferable that a salt of STL is dissolved at a concentration of 2% by mass to 30% by mass with respect to the total amount of the solution. Furthermore, the content of the aqueous component in the aqueous solution is preferably 70% by mass to 98% by mass, and more preferably 90% by mass to 97% by mass, based on the total amount of the aqueous gel composition.

[Others]
The solid succinoyl trehalose lipid composition, the succinoyl trehalose lipid solution, and the emulsified composition according to the present invention are blended with an ultraviolet absorber, an antioxidant, and a fragrance as long as the effects of the present invention are not impaired. Also good.

  The ultraviolet absorber is a substance that is usually used in sunscreen cosmetics and the like, and can reduce ultraviolet A wave, ultraviolet B wave, or both, and reduce harmful effects of ultraviolet rays on the skin.

  Such ultraviolet absorbers include p-aminobenzoic acid, glyceryl-p-aminobenzoic acid, amyl-p-N, N-dimethylaminobenzoic acid, 2-ethylhexyl-pN, N-dimethylaminobenzoic acid. P-aminobenzoic acid derivatives such as methyl 2,4-diisopropylcinnamate, ethyl 2,4-diisopropylcinnamate, potassium p-methoxycinnamate, sodium p-methoxycinnamate, p-methoxycinnamate Cinnamic acid derivatives such as isopropyl acid, 2-ethylhexyl p-methoxycinnamate, 2-ethoxyethyl p-methoxycinnamate, ethyl p-ethoxycinnamate; 2,4-dihydroxybenzophenone, 2,2 ′, 4 , 4'-tetrahydroxybenzophenone, 2-hydroxy-4-methoxy-5-sulfobenzophenone sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone, 2,2′-dihydroxy-4,4′-dimethoxybenzophenone, 2,2′-dihydroxy-4,4′- Benzophenone derivatives such as dimethoxy-5-sulfobenzophenone sodium; salicylic acid derivatives such as 2-ethylhexyl salicylate, phenyl salicylate, salicylic acid-3,3,5-trimethylcyclohexyl; 2- (2′-hydroxy-5′-methoxyphenyl) Examples include benzotriazole and 4-tert-butyl-4′-methoxybenzoylmethane.

  Among these, those that are solid at room temperature can be used by dissolving or dispersing in liquid oil. The liquid or paste-like material at normal temperature can be used as a liquid oil itself, or can be used by mixing with other liquid oils. Among those that can themselves be used as liquid oils, 2-ethylhexyl p-methoxycinnamate or 2-ethylhexyl p-methoxycinnamate in 4-tert-butyl-4′-methoxybenzoylmethane is preferred. is there.

  Antioxidants and fragrances that are solid at room temperature can be used by dissolving or dispersing in liquid oil. The liquid or paste-like material at normal temperature can be used as a liquid oil itself, or can be used by mixing with other liquid oils. Antioxidants that can be used include, for example, tocopherol, tocopherol acetate, and vitamins A (for example, retinoic acid, retinoic acid ester, retinol, retinoid, etc.).

  Applications of the solid succinoyl trehalose lipid composition, succinoyl trehalose lipid solution, and emulsified composition of the present invention preferably include cosmetics, or additives thereof, such as creams, lotions, cleansing gels, cleansing creams, etc. Makeup cosmetics such as foundation, eye shadow, lip color, lip gloss; hair cosmetics such as hair cream, styling gel, hair wax; shampoo, rinse, hand soap, body soap, facial cleansing foam, etc. It can be suitably used as a cleaning material.

  When the solid succinoyl trehalose lipid composition, succinoyl trehalose lipid solution, and emulsified composition of the present invention are used for cosmetics or additives, any ingredients commonly used in cosmetics are added. It can mix | blend in the range which does not impair the effect of invention.

  Examples of such components include hydrocarbons such as petrolatum and microcrystalline wax; esters such as octyldodecyl myristate and isopropyl myristate, glyceryl triisooctanoate and triglycerides such as olive oil; methylphenylpolysiloxane, methyl Silicone oils such as polysiloxane; higher alcohols such as cetanol and behenyl alcohol; fatty acids such as stearic acid and oleic acid; polyhydric alcohols such as glycerin, 1,3-butanediol, and propylene glycol; ethanol, isopropyl alcohol, etc. Lower alcohols: nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, thickeners, UV absorbers, antioxidants, emollients, emulsifiers, Solubilizing agents, anti-inflammatory agents, humectants, preservatives, pH adjusting agents, dyes, perfumes, powders such, and water. Of these, stearic acid and behenyl alcohol are preferred. These preferable contents are 0.01 mass%-10 mass% with respect to cosmetics whole quantity, More preferably, they are 0.1 mass%-5 mass%. The cosmetics thus obtained have little skin irritation and are extremely excellent as cleansing agents, moisturizers, creams and lotions.

  The present invention is not limited to the above-described embodiments, and various modifications are possible within the scope shown in the claims, and embodiments obtained by appropriately combining technical means disclosed in different embodiments. Is also included in the technical scope of the present invention.

  Moreover, all the academic literatures and patent literatures described in this specification are incorporated herein by reference.

  EXAMPLES Hereinafter, although an Example demonstrates this invention more concretely, this invention is not limited at all by these Examples.

[Example 1: Production of STL]
According to the method described in Non-Patent Document 2, Rhodococcus erythropolis SD-74 strain was seed-cultured under the following conditions. The Rhodococcus erythropolis SD-74 strain used in this example was isolated as a vegetable oil-fat assimilating bacterium and was registered with the Patent Organism Depositary at the National Institute of Advanced Industrial Science and Technology as “Accession Number: FERM P-21299” It has been deposited.

100 ml FPY medium in a 500 ml Sakaguchi flask (fructose 2%, polypeptone 0.5%, yeast extract 0.5%, NaNO ₃ 0.1%, KH ₂ PO ₄ 0.1%, MgSO ₄ -7H ₂ O 0. 02%) was inoculated with the bacterial colonies formed on the plate, and cultured with shaking at 30 ° C. for 38 hours.

Per modified MedD medium _{(1L, KH 2 PO 4 5.44g} , K 2 HPO 4 10.45g, KNO 3 3g, MgSO 4 -7H 2 O0.1g, K 2 SO 4 35g, yeast extract 3g, palm oil 100ml The total amount of the culture solution after seed culture was inoculated into 6000 ml, and the main culture was started under the following conditions. Using a 10 L jar culture tank, culture was performed at a culture temperature of 30 ° C. while stirring at 500 rpm. The pH of the medium was set to 7.0, and the pH of the medium was maintained by automatically adding 5N KOH.

[Example 2: Determination of STL]
Sampling was performed about once every two days from the medium during the main culture, and the STL concentration in the culture was quantified as follows.

  The sampled culture solution was centrifuged at 15000 rpm for 10 minutes, and the aqueous layer was extracted with care so that the upper oil component was not mixed. After the extracted liquid was appropriately diluted, the trehalose concentration was quantified by the following anthrone sulfate method. First, 5 ml of anthrone reagent (prepared at the time of measurement by dissolving anthrone in 75% sulfuric acid at a concentration of 0.2%) was added to 1 ml of the diluted sample and stirred. The stirred solution was reacted in boiling water for 10 minutes, quenched with ice for 5 minutes, and allowed to stand at room temperature for 20 minutes.

  The absorbance at a wavelength of 620 nm was measured for the obtained reaction solution. A standard obtained by diluting 4 mM trehalose 20 times was used. Since STL contains one molecule of trehalose, the STL concentration was quantified as the trehalose concentration. The mass concentration of STL was calculated by setting the molecular weight of STL to 840. The results are shown in FIG. After 120 hours from the start of cultivation, 600 ml of palm oil was added, and after 200 hours, 300 ml of palm oil was further added. As shown in the time course of STL production in FIG. 1, the STL concentration of the culture solution reached 37 g / L after 285 hours of culture.

[Example 3: Separation and purification of STL]
860 ml of the culture solution obtained after 285 hours of culture obtained in Example 1 was centrifuged at 6000 rpm for 30 minutes to remove cells and residual substrate in the solution, and then 40 ml of 6N HCl was added to adjust the pH of the solution to 2 .98. A white gel-like precipitate was deposited in the solution. As a result of removing the liquid layer by centrifuging this solution at 6000 rpm for 30 minutes, a precipitate having a wet weight of 182 g was obtained.

  186 g of ethyl acetate was added to the resulting precipitate and stirred thoroughly. The solution separated into the aqueous layer and the ethyl acetate layer was separated using a separatory funnel, and the upper ethyl acetate layer was recovered. Ethyl acetate was removed from the collected ethyl acetate solution using an evaporator. After suspending the solid substance obtained by removing ethyl acetate with an equal amount of hexane, the process of centrifuging the suspension to remove hexane was repeated three times. The liquid obtained by removing hexane was dried with an evaporator to obtain 12.6 g of a white solid. When this white solid was added to distilled water at a concentration of 50 g / l and neutralized by adding NaOH, the white solid began to dissolve in distilled water. When the solution was further sonicated, the white solid was completely dissolved in distilled water after about 5 minutes.

  Further examination revealed that the white solid obtained by the above-described method could be uniformly dissolved in the aqueous solution even at a concentration of 7.5%.

[Comparative Example 1: Separation and purification of STL according to prior art]
The culture solution obtained in Example 1 was centrifuged at 6000 rpm for 30 minutes to remove cells and residual substrate in the solution, and then 6N HCl was added to adjust the pH of the solution to 3 or less. To the precipitated acid precipitate (271.5 g), sterilized water (500 mL) was added and suspended well, followed by centrifugation, and the supernatant was discarded to wash the acid precipitate. The wet weight of the acid deposit after washing was 125.9 g.

  Next, 500 mL of methanol was added to completely dissolve the acid precipitate, and 500 mL of hexane was added and stirred well, and then the methanol layer was separated. This operation using hexane was repeated three times. Finally, methanol was distilled off to obtain a light brown solid (17.1 g). The STL solid (light brown) produced by the conventional method described above did not dissolve in the aqueous solution even at a concentration of 1%. Hereinafter, the STL solid material produced by the above-described method (hereinafter referred to as the new method STL) and the STL solid material produced by the conventional method (hereinafter referred to as the conventional method STL) will be compared in more detail. .

[Example 4: Measurement of absorption spectrum of STL solution]
As described above, the STL solid produced by the above method was white, unlike the STL solid produced by the conventional method. In order to observe this quantitatively, the absorption spectrum in the visible light region of the STL solution was measured.

  The new method STL and the conventional method STL were each dissolved in methanol to prepare an STL solution having a concentration of 5% by mass. Although the new method STL was completely dissolved in methanol, the conventional method STL was not completely dissolved in methanol, and some components remained undissolved. Therefore, the conventional STL solution was passed through a filter (0.45 μm) to obtain a transparent liquid.

  The absorption spectrum in the visible light region of the STL solution prepared as described above was measured using a spectrophotometer (UV-2550, Shimadzu Corporation). The results are shown in FIG. As shown in FIG. 2, the absorbance of the new method STL solution did not exceed 0.05 in all regions. On the other hand, in the conventional method STL, the absorbance was high (greater than 0.05) in the wavelength region of 550 nm or less. That is, the new method STL was white, and it was quantitatively shown that the conventional method STL was colored.

[Example 5: Measurement of light transmittance of STL aqueous solution]
An STL aqueous solution having a pH of 7.0 and a concentration of 1% (w / v) was prepared using the new method STL. Similarly, the conventional method STL was also mixed with pure water in an amount of 1% (w / v) at pH 7.0. However, the conventional method STL was not completely dissolved in water at a concentration of 1% (w / v).

  Next, each light transmittance in wavelength 660nm was measured using the said spectrophotometer. As a result, the light transmittance at a wavelength of 660 nm was 99.5% for the aqueous solution of the new method STL and 4.8% for the mixed solution of the conventional method STL. It has been shown that the new method STL can form an aqueous solution having a very high transparency compared to the conventional method STL.

[Example 6: Measurement of water retention capacity of STL]
The water holding capacity of the STL obtained in Example 3 was measured by the following method. First, 5 mass% aqueous solution of STL (sodium salt) was prepared by dissolving STL in distilled water at a concentration of 5 mass% and adjusting the pH to 7.0 with NaOH. As a comparative control, a 5% by mass aqueous solution of glycerin was prepared.

Next, 10 μl of each 5% by mass aqueous solution prepared as described above was infiltrated into the filter paper cut to 5 × 5 cm, and the mass change of the filter paper was measured up to 8 minutes per minute. The change in mass over time was graphed, and the water evaporation rate was calculated from the slope of the graph. Furthermore, the mass change of the filter paper in which only water was infiltrated was measured, and the calculated inclination was set to 100, and the relative water evaporation rate was calculated. From the relative water evaporation rate, the relative water retention capacity was calculated by the following equation. The results are shown in Table 1.
Relative water retention capacity (%) = 100-relative water evaporation rate

  As shown in Table 1, the STL obtained according to the present invention showed higher water retention capacity than glycerin, which is generally known to have water retention capacity. That is, since the STL manufactured by the manufacturing method according to the present invention has a high water retention capacity, it can be suitably used as a moisturizing agent, for example, for cosmetic use.

[Example 7: Hard water resistance test of STL]
The STL hard water resistance test obtained in Example 3 was conducted by the following method. First, a 0.1 mass% STL aqueous solution (pH 7.0) was prepared. As a comparative control, a 0.1% by mass aqueous solution of sodium linear alkylbenzene sulfonate (LAS) was prepared.

Next, an aqueous solution having various hardnesses is obtained by adding CaCl ₂ aqueous solution (10% by mass in terms of calcium carbonate) to 80 g of each 0.1% by mass aqueous solution prepared as described above and stirring for 5 minutes. Prepared. About the obtained aqueous solution, the light transmittance in wavelength 650nm was measured. The measurement result of the light transmittance is shown in FIG.

  As shown in FIG. 3, the STL aqueous solution did not form an insoluble salt even at a hardness of 300 ppm, and showed very good hard water resistance compared to LAS. That is, since the STL according to the present invention has excellent hard water resistance, for example, it does not cause turbidity or precipitation even in cosmetics in which various components are blended, and thus is suitable as a cosmetic additive or the like. Can be used.

[Example 8: Measurement of foaming force of STL]
The foaming force of the STL obtained in Example 3 was measured by the following method. First, a 0.1 mass% STL aqueous solution (pH 7.0) was prepared. As a comparative control, a 0.1% by mass aqueous solution of sodium linear alkylbenzene sulfonate (LAS) was prepared.

  Next, 50 g of each 0.1% by mass aqueous solution prepared as described above was filled in different mixers. Each aqueous solution was stirred at a rotational speed of 3000 rpm for 15 seconds at a time. FIG. 4 shows the height of the liquid level immediately after each stirring.

  As shown in FIG. 4, the aqueous STL solution hardly generated bubbles even when stirred, indicating that it is a low foaming surfactant compared to LAS. That is, the STL according to the present invention, which is a low-foaming surfactant, can be suitably used as a cosmetic additive or the like because, for example, when used as a component of cosmetics, the product does not foam.

[Example 9: Preparation of emulsion composition]
The STL obtained in Example 3 was dissolved in distilled water while neutralizing with NaOH to prepare an STL aqueous solution containing a sodium salt of STL at a concentration of 0.1% by mass.

  Then, 5 ml of the prepared STL aqueous solution was added to 5 ml of each of the 4 types of oily components described below, and the mixture was treated for 1 minute with a homogenizer (Hiscotron manufactured by Nithion Corporation). As the oil component, squalane (special grade manufactured by Kanto Chemical Co., Inc.), liquid paraffin (first grade manufactured by Wako Pure Chemical Industries), rapeseed oil (MP Biomedicals), and silicone oil (KF-968, Shin-Etsu Silicone) were used. The emulsion compositions thus prepared were all milky white and were stable emulsions that did not separate even after 10 minutes.

  By using the present invention, succinoyl trehalose lipid can be handled as an aqueous solution, and even when used as an additive for any product, the product is not unnecessarily colored. It can be suitably used in the field of manufacturing cosmetics, foods, paints, etc., such as cosmetic moisturizers, emulsifiers, cosmetic water dispersants, food emulsifiers and paint emulsifiers.

FIG. 1 is a diagram showing a time course of STL production. FIG. 2 is a diagram showing an absorption spectrum of the STL solution. FIG. 3 is a diagram showing the results of a hard water resistance test of an STL solution. FIG. 4 is a diagram showing the measurement results of the foaming power of the STL solution.

Claims (13)

  A solid succinoyl trehalose lipid composition obtained by culturing a microorganism in a medium containing a carbon source and capable of dissolving at least 1% by mass in water.   A solid succinoyl trehalose lipid composition obtained by culturing a microorganism in a medium containing a carbon source and white.   The solid succinoyl trehalose lipid composition according to claim 1 or 2, wherein the absorbance of the solution when dissolved in methanol by 5 mass% is 0.05 or less over a wavelength range of 400 nm to 700 nm.   The solid succinoyl trehalose lipid composition according to any one of claims 1 to 3, which is soluble in water by at least 5% by mass.   The solid succinoyl trehalose lipid composition according to any one of claims 1 to 4, wherein the light transmittance at a wavelength of 660 nm of the aqueous solution when dissolved in water by 1% by mass is 95% or more. object.   A succinoyl trehalose lipid solution comprising the solid succinoyl trehalose lipid composition according to any one of claims 1 to 5 dissolved in a solvent.   The succinoyl trehalose lipid solution according to claim 6, wherein the solvent is an aqueous solvent.   A succinoyl trehalose lipid solution, wherein 1% by mass or more of succinoyl trehalose lipid obtained by culturing a microorganism in a medium containing a carbon source is dissolved in an aqueous solvent.   The succinoyl trehalose lipid solution according to claim 8, wherein 5% by mass or more of the succinoyl trehalose lipid is dissolved in the solvent.   The succinoyl trehalose lipid solution according to claim 8 or 9, wherein the light transmittance at a wavelength of 660 nm is 95% or more.   The succinoyl trehalose lipid solution according to any one of claims 7 to 10, further comprising an alkali metal ion or an alkaline earth metal ion.   An emulsified composition comprising the solid succinoyl trehalose lipid composition according to any one of claims 1 to 5, an aqueous component, and an oily component. A step of preparing a succinoyl trehalose lipid aqueous solution by dissolving the solid succinoyl trehalose lipid composition according to any one of claims 1 to 5 in an aqueous solvent, and the succinoyl trehalose lipid aqueous solution and an oil component And a process for emulsifying the emulsion composition.

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