JP2008238157A - Filter for water cleaner containing organic germanium, and method of manufacturing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a filter for a water cleaner containing an organic germanium, and a method of manufacturing the same. <P>SOLUTION: The filter for the water cleaner containing the organic germanium is characterized in that the organic germanium is adsorbed to a porous adsorbent, and the resultant adsorbent is coated with an insoluble polymer. The method of manufacturing the filter for the water cleaner containing the organic germanium includes the step of adsorbing the organic germanium to the porous adsorbent by heating the porous adsorbent, dissolving the organic germanium in distilled water, and mixing the adsorbent with the organic germanium, and the step of coating the porous adsorbent adsorbed with the organic germanium with ethyl cellulose. Therefore, a water containing the organic germanium can be drunk since the organic germanium is continuously dissolved out when cleaning water using the filter for the water cleaner prepared by heating the porous adsorbent to enlarging pores, adsorbing the organic germanium to the enlarged pores, and coating the porous adsorbent adsorbed with the organic germanium with the insoluble polymer. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a water purifier filter containing organic germanium and a method for producing the filter.

  Recently, as the industrial development speed increases rapidly, industrial wastewater generated from various factory facilities and the like flows into a drinking water source, so that the water quality is lowered. Normally, the tap water supplied to each household is taken from the water system of each river through a water intake, and the water contaminated in the water storage is supplied through a water supply pipe through a pollution and water purification process. As a result of using a large amount of disinfecting chemicals in the water purification process, problems caused by foul odors occur when drinking without a separate treatment. Therefore, there is now a general trend to make fresh or purified water edible. However, raw water or purified water is likely to be contaminated with microorganisms and generates an odor.

  Therefore, in order to solve the above problem, a water purifier is used in which a water tank is formed on a synthetic resin material and purified by a fine filter. The water purifier as described above has a problem that it does not further activate components that are good for the human body, such as minerals, and has a bad effect on health due to a very poor gonococcal removing action, bactericidal action and deodorizing action.

  As invented in order to solve the various problems as described above, registered patent publication No. 10-0360283 includes bamboo activated carbon filtration layer, firewood activated carbon filtration layer, pine activated carbon filtration layer, barley stone filtration layer and An active water purifier using a filter produced by laminating germanium ore filtration layers in multiple stages or mixing the above materials at a certain ratio is started.

  Although the water purifier as described above has the advantage of removing the harmful substances in the water and preventing the second pollution of the river water, the filtration filter is formed in multiple stages and the process of treating each material with the filtration layer is required. However, there is a problem that beneficial minerals contained in water are also filtered together through the plurality of filtration layers formed in multiple stages as described above.

  The invention has been made to solve the conventional problems. The purpose of the invention is to adsorb organic germanium to a porous adsorbent to elute organic germanium at the time of water purification with a water purifier to form an insoluble polymer. An object of the present invention is to provide a filter for water purifier containing organic germanium characterized by being coated and a method for producing the filter for water purifier.

  In order to achieve the above object, the present invention relates to a water purifier filter containing organic germanium and a method for producing the water purifier filter.

  More specifically, the present invention is characterized by a filter for water purifier containing organic germanium characterized in that organic germanium is adsorbed on a porous adsorbent and coated with an insoluble polymer.

  The present invention also includes (1) a step of heating the porous adsorbent at 70 to 150 ° C. for 1 to 2 hours, (2) a step of dissolving organic germanium in distilled water, and (3) step (1). (2) mixing the organic germanium-dissolved water in step (2) with the porous adsorbent heated in step (4) and (3) mixing the organic germanium-dissolved water in the temperature range of 25 to 100 ° C. An adsorption step of adsorbing organic germanium on a porous adsorbent consisting of drying for 12 to 24 hours; and (5) an insoluble polymer coating solution by adding distilled water after dissolving the insoluble polymer in ethyl alcohol. A step of coating, a porous adsorbent adsorbed with organic germanium in steps (6) and (4) on an insoluble polymer coating solution, and a porous adsorbent coated in steps (7) and (6) 12 at 70-80 ° C Wherein the manufacturing method of the water purifier filters containing organic germanium which comprises a coating step comprises the step of drying for 24 hours.

  The present invention is characterized in that any one of activated carbon, silica, activated alumina, zeolite, and pillared clay is selected as the porous adsorbent.

  The present invention is characterized in that any one of ethyl cellulose, cellulose nitrate, cellulose acetate, and cellulose propionate is selected as the insoluble polymer.

  Further, the present invention is characterized in that the addition amount of the insoluble polymer is 15 to 25 g.

  Moreover, this invention is characterized by the addition amount of the said porous adsorbent being 5-10 kg.

  Moreover, this invention is characterized by the addition amount of the said organic germanium being 5-10g.

  Moreover, this invention is characterized by the addition amount of the said ethyl alcohol being 5-10L.

  According to the present invention, the porous adsorbent is heated to expand the pores, the organic germanium is adsorbed to the expanded pores, and the organic germanium adsorbed adsorbent is coated with ethyl cellulose and used for the filter of the water purifier. Thus, the organic germanium is eluted in a continuous manner during water purification, and water containing the organic germanium can be drunk daily.

  Hereinafter, the present invention will be described in more detail.

  In the present invention, the porous adsorbent is heated at 70 to 150 ° C. for 1 to 2 hours to expand the pores of the activated carbon to facilitate the adsorption of organic germanium. When the organic germanium is adsorbed by heating, it is preferable to reduce the initial organic germanium elution amount and to allow the organic germanium to be continuously eluted at the time of water purification.

  In order to adsorb organic germanium to the porous adsorbent, solid organic germanium is dissolved in 5 to 15 L of distilled water to produce a liquid.

  In the present invention, the mixed liquid of the porous adsorbent and the organic germanium is dried at 25 to 100 ° C. for 12 to 24 hours. However, such a stage stabilizes the organic germanium adsorbed on the porous adsorbent and moisture. To remove. Preferably, drying is performed at 70 ° C., which is preferable for shortening the drying time and heating the porous adsorbent at 110 ° C. to reduce the pores of the expanded adsorbent.

  In the present invention, ethyl alcohol, ethyl cellulose, and distilled water are added during the production of the insoluble polymer coating solution. At this time, the addition amount of ethyl alcohol is 5 to 10 L, and the addition amount of the insoluble polymer is 15 to 25 g. Yes, the amount of distilled water added is 1-8 L.

  In the present invention, heating is performed at 70 to 80 ° C. for 12 to 24 hours in order to remove alcohol with a mixed liquid of a porous adsorbent adsorbed with organic germanium and ethyl cellulose. Preferably, heating is performed at 60 ° C., which is an alcohol that is used to maintain an excessive temperature in the stage of complete drying to use a porous adsorbent on which organic germanium coated with ethyl cellulose is adsorbed. It has been confirmed that heating and drying at 60 ° C. is preferable because there is a shortcoming in the process in which the drying time becomes longer when drying by heating at room temperature.

  Hereinafter, examples of the present invention will be described in more detail.

  7 kg of activated carbon was heated at 110 ° C. for 1 hour and 30 minutes to expand the pores of the activated carbon, and 7 g of organic germanium was dissolved in 11 L of distilled water. 11 L of water in which organic germanium was dissolved was mixed with heated activated carbon. The mixed organic germanium-dissolved water was dried at 70 ° C. for 24 hours and adsorbed in the expanded pores of activated carbon.

(Comparative Example 1)
In the said Example, it carried out like Example 1 except not having heated activated carbon at 110 degreeC, but adsorbing organic germanium at normal temperature.

(Experimental example 1)
1L, 20L, 100L, 300L, 500L, 1000L, and 1500L of distilled water after filling 70 g of activated carbon adsorbed with organic germanium, which was implemented in Example 1 and Comparative Example 1 above, into a water purifier filter for household use, respectively. The elution amount of organic germanium was compared by passing through.

  The organic germanium elution amount according to Experimental Example 1 is shown in Table 1 below.

  As shown in Table 1 above, in Example 1 according to the present invention, when purified water was passed through a filter in which 1 L of distilled water was heated at 110 ° C. and adsorbed organic germanium was passed at room temperature. Although the amount of organic germanium eluted was less than when organic germanium was adsorbed, the amount of organic germanium eluted increased as the amount of filter passing through increased.

  The above results indicate that the organic germanium is not a porous adsorbent by heating the porous adsorbent at 110 ° C. to expand the pores, rather than adsorbing the organic germanium on the porous adsorbent at room temperature. It can be inferred that it is adsorbed in the enlarged pores.

(Example 2)
7 kg of activated carbon was heated at 110 ° C. for 1 hour and 30 minutes to expand the pores of the activated carbon, and 7 g of organic germanium was dissolved in 11 L of distilled water. Further, 11 L of water in which organic germanium was dissolved was mixed with heated activated carbon. The mixed organic germanium-dissolved water was dried at 70 ° C. for 24 hours and adsorbed in the expanded pores of activated carbon. After dissolving 21 g of ethyl cellulose in 8.5 L of ethyl alcohol, 4.5 L of distilled water was added to produce 13 L of an ethyl cellulose coating solution. The activated carbon on which the organic germanium was adsorbed was coated with 11 L of an ethylcellulose coating solution and dried at 75 ° C. for 20 hours.

(Comparative Example 2)
In Example 2, the same procedure as in Example was performed except that 7 g of ethylcellulose was used.

(Comparative Example 3)
In Example 2, the same procedure as in Example 2 was performed except that 35 g of ethylcellulose was used.

(Experimental example 2)
Elution amount of organic germanium when 15 g of activated carbon adsorbed with organic germanium produced in Example 2, Comparative Example 2 and Comparative Example 3 was filled in the filter and then passed through 1 L, 20 L and 1000 L of distilled water through the filter. Was measured.

  The organic germanium elution amount according to Experimental Example 2 is shown in Table 2 below.

  As shown in Table 2 above, in Example 2 according to the present invention, Comparative Example 2 using 7 g of ethyl cellulose as a coating agent for coating the porous adsorbent adsorbed with organic germanium allowed 1 L of distilled water to pass through. Occasionally, a large amount of organic germanium was eluted at an organic germanium elution amount of 1229 ppb, and when 1000 L was passed, the organic germanium elution amount was rapidly reduced. On the contrary, in Comparative Example 3 using 35 g of ethyl cellulose, the amount of elution of organic germanium was as small as 234 ppb when 1 L of distilled water was passed, but the amount of elution of organic germanium was 1 when 1000 L of distilled water was passed. It can be inferred that if the coating layer is thick, the organic germanium is less likely to be eluted in a continuous manner, showing that it is eluted very little at .80 ppb.

  Therefore, the elution amount of organic germanium of Example 2 according to the present invention showed that the elution amount of organic germanium was maintained more continuously than in Comparative Examples 2 and 3 even when 1000 L of distilled water was passed.

  The present inventor determines whether or not the water that has passed through a domestic water purifier using a filter coated with ethyl cellulose on a porous adsorbent adsorbed with organic germanium conforms to the water quality inspection of edible water prescribed in Korea. Therefore, it is currently prescribed in Korea, and the respective general bacteria (Total colony counts), total coliforms (Falcal Streptococcus), Pseudomonas aeruginosa, Salmonella pH, Salmonella pH It is the following Experimental Example 1 whether it is suitable for the number of Shigella, Spore Forming Sulfite Reducing Anaerobes, and Yersinia. And it tries to prove through 10.

(Experimental Example 1: General bacteria counts)
1. Standard agar medium After adding 5.0 g of tryptone, 2.5 g of yeast extract, 1.0 g of sucrose, and 15.0 g of agar to 1.0 L of water and dissolving them by heating, the pH is adjusted to 7.0 ± 0.2. After adjusting to 10-10 ml each, it was placed in a test tube and autoclaved at 121 ° C. for 15 minutes.

2. Phosphate buffer diluent 34 g of potassium dihydrogen phosphate dissolved in 500 ml of water, adjusted to pH 7.2 ± 0.2 at room temperature with sodium hydroxide solution (0.5 N), then water added to 1 L And make this a stock solution for storage. Phosphate buffer diluted solution containing 1.25 ml of this stock solution for storage and 5.0 ml of magnesium chloride solution prepared by dissolving 81.1 g of magnesium chloride (hexahydrate) in 1 L of water and then making up to 1 L with water make. The phosphate buffer diluted solution was divided into dilution bottles and test tubes with a spiral stopper so that the amount was 99 ± 2 ml, and autoclaved at 121 ° C. for 15 minutes.

3. Instruments and equipment Pipettes or automatic pipettes (using sterilized to 1-5 ml measuring pipettes and automatic pipettes (including plastic tips)), Petri dishes (about 9 cm in diameter and about 1.5 cm in height glassware (65 cm ² ) or a single-use plastic product (57 cm ² sterilized), a constant temperature water bath (use that water temperature can be maintained at 45 ± 0.5 ° C.), an incubator (culture temperature 21 Can be maintained at ± 1.0 ° C), colony counter (using a magnifying glass and a lighting device attached to counter the colonies and easily partitioning the Petri dish into 1 cm ² )

4). Test method a. Preparation and test of test solution The water purified from the water purifier using the filter according to the present invention (hereinafter referred to as “test water”) must be refrigerated so as not to freeze at 4 ° C. or less, and in this case, 12 hours. The analysis must be started within. If the analysis cannot be started within 12 hours, the general bacteria of the purified water will not be tested.

  I. Dilute the test water to a suitable concentration (concentration that can be estimated to be 30-300 bacteria per ml) using a phosphate buffer dilution or peptone dilution and dilute 1 ml of each step dilution. Place in two or more sterilized petri dishes.

  C. 10 ~ 12ml of R2A medium sterilized in advance and maintained at 45 ± 0.5 ° C is aseptically divided into petri dishes containing test solutions, and rotated left and right so that the culture medium and test water mix well. To do.

  D. Once the medium has solidified, calculate the number of colonies formed by culturing at 21 ± 1.0 ° C. for 72 ± 3 hours.

  E. The diluted solution sterilized in the control group test is experimented in the same manner as described above to make a control group.

  F. The operation time from diluting the test water to putting the plate medium into the Petri dish should not exceed 20 minutes.

5. Calculation of the number of bacterial colonies a. As a general rule, a colony counter is used immediately after culturing to select a plate having 30 to 300 colonies per plate and count the number of colonies.

I. When 300 or more colonies are formed for each flat plate, the most representative flat plate is selected and the number of colonies is calculated by the dense plate measurement method. When using a glass petri dish with an internal diameter of 9 cm, count the number of colonies in 1 cm ² to 13 places, multiply the average number of colonies by 65, and use a plastic petri dish for one time. Multiply 57.

  C. When 30 or less colonies are formed on each plate, the plate colonies inoculated with the stock solution are counted and averaged, and the number is always called CFU (Colony Forming Unit) in ml.

  D. When there is no flat plate having 30 to 300 colonies and one or more flat plates having 300 or more colonies are present, the plate colonies closest to 300 are counted.

  E. The calculation method is to calculate the number of colonies in each plate used for the corresponding dilution factor, divide it into the number of plates used after combining and calculate the average number of colonies per plate, and multiply this by the corresponding dilution factor The numerical value is the number of low-temperature general bacteria, and the number of CFU in ml is described. In all the counting processes, when the number of low-temperature general bacteria is 100 or more, the number of 3 or less from the high unit number is rounded off, the effective number is cut into 2 stages, and the value obtained by subtracting 0 from that is the number of general bacteria in 1 ml. When the number of general bacteria is less than 100, the number discarded after the decimal point is described as the number of general bacteria in 1 ml.

(Experimental example 2: Total E. coli)
1.2 Lactose medium after drainage (estimated test medium)
After adding 6.0 g of beef extract, 10.0 g of peptone, and 10.0 g of lactose to 1 L of water and dissolving it by heating, the pH is adjusted to 6.9 ± 0.2, and then a Durham test tube is added. 10 ml each was placed in a test tube and autoclaved at 121 ° C. for 15 minutes.

2. BGLB (Brilliant Green Lactose Bile) medium (determined test medium)
After 10.0 g of bile bile (20.0 g), peptone (10.0 g), lactose (10.0 g) and brilliant grin (0.0133 g) are dissolved by heating and adjusted to a pH of 7.2 ± 0.2. Place 10 ml each in a small test tube containing a Durham test tube and autoclav it at 121 ° C for 15 minutes.

3. Instruments and equipment Pipettes (use sterilized as 5-10 ml measuring pipettes and automatic pipettes), test tubes (small test tubes (inner diameter 16 mm, height 150 mm) and medium test tubes (inner diameter 18 mm, height 180 mm) 2 types of test tubes can be plugged with cotton (plastic and metal plugs can also be used) and can be sterilized with high-pressure steam.) Durham test tube (Durham test tube (inner diameter 6 mm, height 30 mm) And a platinum loop (using a platinum loop with an inner diameter of about 3 mm) and an incubator (use that the culture temperature can be maintained at 35 ± 0.5 ° C.).

4). test
(1) Estimation test a. Estimated test for drinking water is to inoculate 10 medium test tubes each containing 10 ml of double concentrated lactose medium or lauryl tributos medium (test tubes containing Durham test tubes) with 35 ml of test water. After incubating at 0.5 ° C for 24 ± 2 hours, if gas is generated, perform a deterministic test by judging from a positive estimated test (in 5 large test tubes containing 10 ml of 3 times concentrated medium). 20 ml of water can be inoculated).

  I. If no gas is generated in any of the test tubes after 24 ± 2 hours of culturing, if no gas is generated even if the cells are cultured up to 48 ± 3 hours under the same conditions, the estimated test is judged as negative. When gas generation is observed from the above test tubes, a definite test is performed.

(2) Final test a. When gas is generated from the estimated test, inoculate each test tube containing 10 ml of BGLB medium (test tube containing the Durham test tube) with one platinum loop from each of the test tubes where the gas is generated. Incubate at 35 ± 0.5 ° C. for 48 ± 3 hours.

  I. At this time, if gas is not generated, the definite test of the total coliform group is determined to be negative, and if gas generation is observed, the total coliform group is determined to be positive.

(Experimental example 3: Fecal Streptococcus group)
1.3 times concentrated azido sucrose medium (estimated test medium)
After 13.5 g of beef extract, 15.0 g of beef extract, 45.0 g of tryptone, 22.5 g of sucrose, 22.5 g of sodium chloride and 0.6 g of sodium azide were dissolved by heating, the pH was 7.2 ± 0.2 at room temperature. After adjusting so that it becomes, it divides into 25 ml at a time in a large test tube, and sterilizes by autoclave for 15 minutes at 121 degreeC.

2. Pfizer enterococcus selective agar medium (confirmation test medium)
1 L of water, 17.0 g of peptone C, 3.0 g of penton B, 5.0 g of yeast extract, 10.0 g of bacteriological bile powder, 5.0 g of sodium chloride, 1.0 g of sodium citrate, 1.0 g of esculin, ammonium citrate Add 0.5 g of iron, 0.25 g of sodium azide and 15.0 g of agar and heat to dissolve and adjust the pH to 7.1 ± 0.2 at room temperature. After steam sterilization, after cooling to 50 to 55 ° C., aseptically divide into approximately 15 ml each into a sterilized petri dish and solidify to prepare a plate medium.

3. Appliances and equipment Use the same appliances and equipment as those used for the gonococcus group test.

4). test
(1) Estimated test After inoculating 50 ml of test water into a large test tube containing 5 ml of 3 times concentrated azide sucrose medium each and then culturing at 35 ± 0.5 ° C. for 24 ± 2 hours and becoming turbid If this is not observed, extend the culture to 48 ± 3 hours. After culturing, the medium is observed for turbidity. If any of the tubes is not thin, the estimated test is negative, and if one or more tubes are confirmed to be thin, the estimated test is positive regardless of bacterial growth or sample contamination. Judge and perform the next definitive test.

(2) Confirmation test Take 1 platinum loop from all test tubes that were confirmed to be thin in the estimation test and streak them to Pfizer enterococcus selective agar medium at 35 ± 0.5 ° C for 24 ± 2 hours. Incubate. After culturing, colonies are observed, but if a blackish brown colony with brown frames is formed, the definitive test is positive, that is, if the colony is determined to be positive for stool streptococci and the definitive test is not observed Is negative.

(Experimental example 4: Pseudomonas aeruginosa)
1.3 times concentrated asparagine medium (estimated test medium)
Add 19.0 grams of asparagine DL, 3.0 grams of potassium monohydrogen phosphate, and 1.5 grams of magnesium sulfate (7-hydrate) to 1 liter of water and dissolve it with heating to adjust the pH to 7.0 ± 0.2 at room temperature. After that, 25 ml each is put into a large test tube and autoclaved at 121 ° C. for 15 minutes.

2. Acetamide medium (medium for confirmation test)
In 1 L of water, put 10.0 g of acetamide, 5.0 g of sodium chloride, 1.39 g of potassium monohydrogen phosphate, 0.73 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate (7-hydrate) and 0.012 g of phenol red After heating and dissolving to adjust the pH to 7.0 ± 0.2 at room temperature, aliquot 10 ml each into a small test tube and autoclaving at 121 ° C. for 15 minutes.

3. Tryptic Soy Enrichment Medium Add 17.0g of casein pancreatic juice degradation product, 3.0g soybean powder papain degradation product, 5.0g sodium chloride, 2.5g dipotassium phosphate and 2.5g sucrose to 1L water Dissolve. Sterilize by autoclaving at 121 ° C. for 15 minutes, and after aseptically separating about 15 ml each into a sterilized petri dish, solidify.

4). Appliances and equipment Using the same test as that of the gonococcal group test, an ultraviolet lamp (irradiation with a long wavelength of 365 nm) and UV protection glasses are required.

5. Test method
(1) Estimated test Each 50 ml of test water is inoculated into 5 test tubes each containing 25 ml of 3 times concentrated asparagine medium. Asparagine medium inoculated with test water is cultivated at 35 ± 0.5 ℃ for 24 ± 2 hours, then put on UV protective glasses in the dark room and irradiate with long-wave ultraviolet light (365 nm neighborhood) with an ultraviolet lamp. The presence or absence of fluorescence is observed, and if fluorescence occurs, the estimation test is made positive. However, when clear fluorescence is not confirmed, culture is further continued and observed for 48 ± 3 hours. At this time, the absence of green fluorescence in the test tube is confirmed negative for Pseudomonas aeruginosa. If green fluorescence is recognized in the test tube, the following definitive test is subsequently performed to confirm the presence or absence of Pseudomonas aeruginosa as a positive estimation test.

(2) Confirmation test In all the test tubes that confirmed greenish fluorescence from the estimation test, immediately take 3 times (about 0.1 ml) from the upper layer of the positive medium using a platinum loop and place on the slope of each acetamide medium. The cells are transplanted and cultured at 35 ± 0.5 ° C. for 24-36 hours. If there is no significant color change in the medium after culturing, it is negative for Pseudomonas aeruginosa. However, if a single test tube shows magenta, it is determined that the definite test is positive.

(3) Confirmation test After confirming positive colonies, the cells are cultured in a Tryptic Soy enrichment medium or a similar medium at 41.5 ± 0.5 ° C. for 48 hours and observed for growth. Colonies that do not grow are determined to be negative for Pseudomonas aeruginosa, and grown colonies are confirmed to be Gram-negative bacilli by microscopic observation after Gram staining, and then identified using a standardized identification test Kit such as API20NE. Or, check the biochemical characteristics described below. If the biochemical confirmation test identifies Pseudomonas aeruginosa, it is determined to be positive for Pseudomonas aeruginosa.

(Experimental Example 5: Spore Forming Sulfite Reducing Analyzes)
1.2 times concentrated DRCM medium 6.0 g yeast extract, 20.0 g beef extract, 20.0 g tribute case, 10.0 g sucrose, 10.0 g sodium chloride, 2.0 g soluble starch, cysteine (hydrochloride) 1 0.0 g, 6.0 g of sodium acetate and 0.5 g of agar are added and dissolved by heating to adjust the pH to 6.8 ± 0.2 at room temperature, and then 10 ml each in a medium test tube with a helical stopper. Separate and autoclaving at 121 ° C for 15 minutes. At the time of the experiment, 0.4 ml each of a mixture of 4% ferric citrate and 7% sodium phosphite sterilized by filtration in the same amount is put in each test tube.

2. Appliances and devices The appliances and devices are the same as those of the gonococcal group test, and additionally require a constant temperature water bath (use that the water temperature can be maintained at 75 ± 1.0 ° C.).

3. Test method
(1) Apo formation test After 10 ml of test water is aseptically put into a test tube with 5 spiral stoppers, it is placed in a constant temperature water bath maintained at 75 ± 1.0 ° C. for 10 minutes. At this time, all the bacteria that cannot form spores are killed, and only bacteria that can form spores can live.

(2) Cultivation and nitrous acid reducing power test A test tube with five spiral stoppers placed in a constant temperature water bath at 75 ± 1.0 ° C. for 10 minutes is immediately cooled to about 20 ° C. and then prepared in advance. Add 10 ml each of the 2 × concentrated DRCM medium. Then, after mixing the test water and the medium so that no bubbles are generated, 2-3 ml of liquid paraffin sterilized in advance is aseptically placed on the surface of the medium and the stopper is tightly sealed. Incubate at 35 ± 0.5 ° C. for 48 ± 3 hours. After culture, when the medium turns black, it is determined that nitrous acid has been reduced, and it is determined that nitrous acid-reducing anaerobic spore-forming bacteria are positive. If one of the five test tubes is positive, it is detected. It is determined that

(Experimental example 6: Salmonella typi)
1.3 times concentrated selenite liquid enrichment solution 15.0 g of tryptone, 12.0 g of lactose, 12.0 g of selenite and 30.0 g of sodium phosphate were dissolved in 1 L of water and dissolved at 60-70 ° C. without boiling. Then, aseptically put 25 ml each into a sterilized large test tube and cool. The dispensed medium is stored at 2-8 ° C.

2. Bismuth sulfite selective medium-for presumed test 1 L of peptone 10.0 g, beef extract 5.0 g, sucrose 5.0 g, sodium monohydrogen phosphate 4.0 g, ferrous sulfate 0.3 g, bismuth sulfite 8.0 g, Bring 0.025 g of brilliant green and 20.0 g of agar and dissolve with heating, then cool to 50-55 ° C. and separate into solid Petri dishes to solidify. Do not keep boiling for more than 1-2 minutes during heating. The prepared medium is stored at 2-8 ° C. and is not stored for more than 4 days.

  3. The equipment and apparatus are the same as those used for the gonococcus group test, and additionally require a refrigerator (2 to 5 ° C.), an optical microscope, a gas burner, an alcohol lamp, and a slide glass.

4). test
(1) Estimated test 50 ml of test water is inoculated into 5 test tubes each containing 25 ml of 3 times concentrated selenite enrichment medium. The selenite medium inoculated with the test water is cultured at 37 ° C. for 18 to 24 hours. If the culture time is extended, other bacteria such as Oyster fungus grow, so strictly observe the culture time. Take the enrichment medium culture from all tubes using the platinum loop and streak into the Bizmouth agar selective medium. Colonies are observed after culturing at 35 ° C. for 24 hours, and cultivated again and observed again 48 hours later, and a black glittering colony with a white ring at the end is estimated to be positive for Salmonella. When there is no typical positive colony and a green colony is formed, the colony is picked up and tested.

(2) Confirmation test a. Biochemical confirmation test: Colonies positively determined in the estimation test are purely isolated and repopulated in a selective medium. After Gram staining, it is confirmed that it is Gram-negative and Neisseria gonorrhoeae by microscopic observation, and API20E Kit is used. If it is identified in Salmonella in the biochemical confirmation test, it is determined that Salmonella is positive. The experiment ends with a positive test, and if more information on pathogenicity and ease is obtained, more detailed species and serotype identification is performed through serological tests.

  I. Serological test: The bacteria identified in Salmonella in the biochemical test are determined by observing the antiserum reaction of the A, B, C, D, and E groups corresponding to the Salmonella 0 antiserum. Test with the single element antiserum to which it belongs. If the Vi antiserum has aggregation only, confirm that it is not Salmonella typhi, boil the bacterial suspension at 100 ° C. to destroy the Vi antigen, and then determine the group by looking at 0 antiserum aggregation. After testing flagellar antigens Phase I and II with H antiserum, 0 antigen and flagellar antigen are combined to determine Salmonella species. In addition, if the Salmonella species, ie, species or serotype, are to be confirmed in more detail here, the final identification will be confirmed by requesting the National Health Service Salmonella Center. In the present invention, it is determined only whether or not the Salmonella species is compatible with the number of drinking water bacteria in Korea.

(Experimental example 7: Shigella)
The 1.3 times concentrated selenite liquid enrichment medium is produced in the same manner as the Salmonella enrichment medium.

2. XLD (Xylose Lysine Deoxycholate)) agar selective medium (for estimation test)
Yeast extract 3.0 g, L-lysine 5.0 g, xylose 3.75 g, lactose 7.5 g, sucrose 7.5 g, sodium desoxycholate 2.5 g, citric acid diiron ammonium 0.8 g, Add 6.8 g sodium thiosulfate, 5.0 g sodium chloride, 15.0 g agar and 0.08 g phenol red and heat until completely dissolved. After cooling to 55-60 ° C., the cells are separated into a sterilized Petri dish and solidified. The dispensed medium is stored at 2-8 ° C.

  3. The same instrument and apparatus as those used in the Salmonella experiment method are used.

4). test
(1) The enrichment process of the estimation test is the same as that of Salmonella. Take the cultured enrichment medium with a platinum loop, streak it on the XLD agar selective medium in the same manner as Salmonella, and then incubate at 35 ° C for 24 hours to form a red colony. judge.

(2) Confirmation test a. Biochemical confirmation test: The same test as that for Salmonella.

  I. Serological test: If you want to know the species of strains identified in Shigella from the biochemical confirmation test, do the Shigella 0 antiserum agglutination test.

(Experimental example 8: Yersinia)
1. CIN agar medium The constituents of Elsiacia selective agar basic medium are 2 g yeast extract, 17 g peptone, 3 g protoauspeptone, 20 g mannitol, 0.5 g sodium deoxycholate, 0.5 g sodium chlorate, 1 g sodium chloride, pyruvin It consists of 2 g of sodium acid, 10 mg of magnesium sulfate (hexahydrate), 13.5 g of agar, 30 mg of neutral red, 1 mg of crystal violet, 4 mg of irgasan and 1 L of water.

  The components of the CIN Ercilia antimicrobial replenisher are 4 mg cefulodin, 2.5 mg novobiocin and 10 ml water. The Ercinia selective agar basal medium is autoclaved at 121 ° C. for 15 minutes, then cooled to 45-50 ° C., and a CN Elsia antimicrobial supplement is added.

2. MacConkey Agar Peptone 17g, Proteose Peptone 3g, Lactose 10g, Bile Salt No. 31.5 g, sodium chloride 5 g, agar 13.5 g, neutral red 0.03 g, crystal violet 0.001 g and water 1 L (final pH (25 ° C.) 7.1 ± 0.2)

3. mMC medium D-sorbitol 10 g / L, irgaic acid 4.0 mg / L and novobiocin 2.5 mg / L are added to the above McConkey agar base.

4.0.288% Potassium Hydroxide Solution 8.5 g of sodium chloride, 2.88 g of potassium hydroxide and 1 L of water are mixed.

5. Instruments and devices Filtration membranes (use a sterilized filter membrane with a pore size of 0.45 μm and a diameter of 47 mm, sterilized with a lattice drawn on one side of the membrane), filtration devices (with the filtration membrane in between A device capable of high-pressure steam sterilization is used as a device that allows filtration, and a circular absorbent pad (a circular pad having a diameter of 47 mm is sterilized and used as a thick room or sponge or glass fiber filtration membrane). ), Petri dish (use a sterilized small product (24 cm ² ) with a diameter of about 5.5 cm and a height of about 1.2 cm), tweezers (Use a flame sterilizable form that does not cause damage.)

6). Test a. Estimated test After collecting 2 L of test water using a sterile container, test immediately. If it is not possible to test immediately, bring it to a refrigerated storage condition and test it in a timely manner. 1 L of the collected sample water is passed through a sterile filter paper (0.22 μm pore size) using a sterilized filtration device, and then continuously from 0.288% potassium hydroxide solution 20 to 20 After 30 ml is completely filtered within 20 seconds, this filter paper is placed on CIN medium as it is and cultured at 25 to 28 ° C. for 48 hours. The remaining 1L is cultured in a MacConkey medium (or mMC medium) through the same process. In CIN medium, small colonies that are not mucous and have a deep red center, and in MacConkey medium (or mMC), avoid red or mucous colonies and are small (1 to 2 mm in diameter), flat colorless or light pink And transfer to a brain heart fusion medium (Brain Heart Infusion Agar).

I. Preliminary identification Preliminary experiments are performed by inoculating TSI medium, element medium, and motility medium. In preliminary experiments, colonies that do not have the characteristics of Ercilia are determined to be negative, and isolated colonies that have the characteristics of Ercilia are negative upon Gram staining. Perform a specific identification test. When culturing at 37 ° C. for 24 hours in TSI medium, enterocolitica decomposes sucrose and yellows the bottom and the slope. In pseudotuberculosis, the slope is yellowed, the bottom is not yellowed, and remains red. Gas and hydrogen sulfide are not generated. In the elemental medium, a positive red color appears, and in the motility test, two motility mediums were inoculated and cultured separately at 37 ° C and 25 ° C, respectively. Wow.

C. Confirmation Identification Test API 20E Kit is used for the biochemical identification test.

  In the above experimental examples 1 to 8, whether or not the water purifier water that has passed through the water purifier using the porous adsorbent adsorbed with organic germanium according to the present invention as an filter conforms to the Korean drinking standard is determined. It is shown in Table 3 below.

  As shown in Table 3 above, compared with the examples according to the present invention and the Korean standards, the water that passed through the filter according to the present invention appeared to meet the Korean standards. Therefore, it was proved that the organic germanium adsorbent and ethyl cellulose do not affect the pollution degree of the water purifier.

  In addition, the Korean standards further include gonococcal and fecal coliforms. However, the present invention was not tested on gonococcal and fecal coliforms in total E. coli, which is suitable for Korean standards.

  In addition, the present inventor, when water was passed using a water purifier including an adsorbent adsorbed with organic germanium and a filter containing ethyl cellulose, lead, fluorine stipulated as health hazardous substances from Korean drinking standards The contents of arsenic, selenium, mercury, cyanide, ammoniacal nitrogen, hexavalent chromium, acidic nitrogenous nitrogen, cadmium and boron were measured.

  The experimental methods of the following Experimental Examples 9 to 19 using Example 2 of the present invention are based on the drinking water quality process test method of Environmental Prefectural Notification No. 2002-91 (revised 2002.6.21).

Experimental Example 9: Lead (Pd; lead)
According to the drinking water quality process test method 2-2-2-1 of Environmental Prefectural Notification No. 2002-91.

Experimental Example 10: Fluoride (F)
According to the drinking water quality process test method 2-2-1-1 of Environmental Notification No. 2002-91.

Experimental Example 11: Arsenic (As; Arsenic)
According to Environmental Water Notification 2002-91, drinking water quality process test method 2-2-2-2.

Experimental Example 12: Selenium (Se; Selenium)
According to Environmental Water Quality Notification 2002-91, drinking water quality process test method 2-2-2-3.

Experimental Example 13: Mercury (Hg; Mercury)
According to Environmental Water Notification 2002-91, Drinking Water Quality Process Test Method 2-2-2-4.

Experimental Example 14: Cyanide (CN)
According to the drinking water quality process test method 2-2-1-2 in the environmental notification No. 2002-91.

Experimental Example 15: ammonia nitrogen _{(NH 3 -N, Ammonium-Nitrogen} )
According to the drinking water quality process test method 2-2-1-3 in Environmental Notification No. 2002-91.

Experimental Example 16: Hexavalent chromium (Cr ⁶⁺ ; Hexachrome)
According to Environmental Water Quality Notification 2002-91, drinking water quality process test method 2-2-2-5.

Experimental Example 17: Nitrogen acid nitrogen (NO ₃ -N; Nitrite Nitrogen)
According to Environmental Water Quality Notification 2002-91, drinking water quality process test method 2-2-1-4.

Experimental Example 18: Cadmium (Cd; Cadmium)
According to Environmental Water Quality Notification 2002-91, drinking water quality process test method 2-2-2-6.

Experimental Example 19: Boron (B)
Drinking water quality process test method of Environmental Prefectural Notification No. 2002-91 2-2-2-12 Judo coupled plasma-atom emission method.

  Table 4 below shows whether or not the experimental results for Experimental Examples 9 to 19 are compatible with the Korean drinking standards.

  As shown in Table 4 above, the present invention does not affect health harmful inorganic substances even when coated with a porous adsorbent adsorbed with organic germanium and used in a filter, and safely contains water containing organic germanium. It was proved that it can be drunk.

As described above, the present invention is not limited to the above-described specific preferred embodiments, and based on the basic concept of the present invention claimed from the claims, those who have ordinary knowledge in the technical field, Various implementation variations are possible, and such variations are within the scope of the claims of the present invention.

The figure which showed the manufacturing-process figure of the filter for water purifiers containing the organic germanium by this invention.

Claims (13)

  A filter for water purifier containing organic germanium, wherein organic germanium is adsorbed on a porous adsorbent and coated with an insoluble polymer.   2. The water purifier filter according to claim 1, wherein the porous adsorbent is selected from activated carbon, zeolite, silica, activated alumina, and pillared clay.   3. The water purifier filter containing organic germanium according to claim 1, wherein any one of ethyl cellulose, cellulose nitrate, cellulose acetate, and cellulose propionate is selected as the insoluble polymer.   The water purifier filter containing organic germanium according to claim 1, wherein the amount of the porous adsorbent added is 5 to 10 kg.   The water purifier filter containing organic germanium according to claim 1, wherein the amount of the insoluble polymer added is 15 to 25 g.   The filter for a water purifier containing organic germanium according to claim 1, wherein the amount of the organic germanium added is 5 to 10 g. (1) heating the porous adsorbent at 70 to 150 ° C. for 1 to 2 hours;
(2) dissolving organic germanium in distilled water;
(3) mixing the porous adsorbent heated in step (1) with water in which organic germanium is dissolved in step (2);
(4) an adsorption step for adsorbing organic germanium on a porous adsorbent comprising drying the water in which organic germanium mixed in step (3) is dissolved at 25 to 100 ° C. for 12 to 24 hours;
(5) dissolving an insoluble polymer in ethyl alcohol and then adding distilled water to produce an insoluble polymer coating solution;
(6) coating an insoluble polymer coating solution with a porous adsorbent adsorbed with organic germanium in step (4); and
(7) The production of a filter for water purifier containing organic germanium, comprising a coating step comprising a step of drying the porous adsorbent coated in step (6) at 70-80 ° C. for 12-24 hours Method.   The method for producing a water purifier filter containing organic germanium according to claim 7, wherein the porous adsorbent is selected from activated carbon, zeolite, silica, activated alumina, and pillared clay. The method for producing a water purifier filter containing organic germanium according to claim 7, wherein the insoluble polymer is selected from ethyl cellulose, cellulose nitrate, cellulose acetate, and cellulose propionate. The method for producing a filter for a water purifier containing organic germanium according to any one of claims 7 to 9, wherein the addition amount of the porous adsorbent is 5 to 10 kg.   The method for producing a water purifier filter containing organic germanium according to any one of claims 7 to 9, wherein the amount of the insoluble polymer added is 15 to 25 g.   The method for producing a water purifier filter containing organic germanium according to any one of claims 7 to 9, wherein the amount of the organic germanium added is 5 to 10 g.   The method for producing a water purifier filter containing organic germanium according to any one of claims 7 to 9, wherein the amount of ethyl alcohol added is 5 to 10 L.

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