JP2008231429A - Antioxidants and external preparations for skin - Google Patents

Abstract

An antioxidant and an external preparation for skin having excellent antioxidant ability and whitening action are provided.
An anti-oxidant comprising an extract of crow ear grass or small scabbard and an external preparation for skin containing the extract.
[Selection figure] None

Description

  The present invention relates to an antioxidant and an external preparation for skin, and in particular, an excellent antioxidant capable of being added to pharmaceuticals, quasi drugs, cosmetics, foods, etc. The present invention relates to an external preparation for skin having antioxidant ability and whitening action.

  Conventionally, synthetic antioxidants such as BHT and BHA, various antioxidants such as α-tocopherol and ascorbic acid, and various plant extracts are used in cosmetics, pharmaceuticals, foods and the like. However, there were some concerns in terms of safety, and the effects were still not satisfactory.

  In view of such circumstances, the present inventors have conducted extensive research to improve the difficulties of the prior art, and as a result, the extract of crow ear grass or small husk has an excellent antioxidant effect, and further a whitening effect. As a result, the present invention has been completed.

  That is, an object of the present invention is to provide an antioxidant and a skin external preparation having high safety and excellent antioxidant ability.

  The present invention achieves the above-mentioned object by providing an antioxidant characterized by comprising an extract of crow ear grass or small scabbard and an external preparation for skin containing the extract.

  The antioxidant and the external preparation for skin according to the present invention have excellent antioxidative ability and whitening action, and prevent skin aging including blotches, buckwheat, dullness, wrinkles, If so, it is very useful.

  The crow ear grass used in the present invention is also referred to as a crow ear grass, a short tube crow ear plant (Lagotis brevituba Maxim.), A large crow ear plant (Lagotis clarkei Hook. F.), A large tube crow ear plant (Lagotis). maerosiphon Tsong et. HP Yang and Lagotis ramalana Batalin, both of which can be used alone or in combination, and the small husk is called Cortex Berberidis, It is obtained from Bernese Kansuensis Schneid. It has been used as a herbal medicine in Tibet. It has been said that crow ear grass has an antilipidemic action, and small scabbard has an effect on eyestrain.

Examples of the extraction solvent for obtaining the extract of crow ear grass or small husk are various alcohols such as water, lower alcohols such as ethanol, propanol and butanol, 1,3-butylene glycol, propylene glycol, glycerin and dipropylene glycol. And monohydric alcohols or mixtures thereof. Among these, water alone, a mixture of water and ethanol, a mixture of water and 1,3-butylene glycol is preferable, and the ratio of ethanol or 1,3-butylene glycol to water is 1: 1 to 1:99 ( The volume ratio) is more preferable. Extraction may be performed by heating at a low temperature and at a normal temperature. Extraction with bass is preferable from the aspect of appraisal. The obtained extract may be appropriately purified and filtered, or may be concentrated and dried by a conventional method.

  The blending amount of the extract of eel ear grass or small husk in the external preparation for skin varies widely depending on the purpose, target site, etc. and cannot be specified in general, but it is based on the total amount of external preparation for skin In general, the content is preferably 0.001 to 10% by mass (hereinafter simply referred to as%), more preferably 0.01 to 3%.

  The antioxidant according to the present invention can be used by dissolving or dispersing the extract in an oil or a solvent. In addition to appropriate use of excipients generally used for pharmaceuticals, foods and the like, granules and powders can be used, and liquids, emulsions, creams, pastes and the like can be obtained using appropriate solvents. Further, it is more preferable to contain a synergist such as ascorbic acid, citric acid, and succinic acid, which is known to exhibit a synergistic effect when used in combination with an antioxidant as necessary.

  Antioxidants of the present invention are not only cosmetics such as lipsticks, milky lotions, creams, pharmaceuticals, quasi drugs, but also animals and plants such as fish oil, lard, soybean oil, linseed oil, corn oil, cottonseed oil, palm oil, etc. It can be widely used in foods such as butter, margarine, mayonnaise, ham, potato chips, fried rice crackers, and fish meat without sacrificing the taste, aroma, touch and the like.

  When the extract according to the present invention is used in cosmetics, it can be blended into various cosmetics such as skin cosmetics, makeup cosmetics, hair cosmetics, bathing agents, etc., and as dosage forms, lotions, creams, ointments, emulsions, papps , Gels, powders, granules and the like. In particular, since the extract of the present invention has an antioxidant action, it not only prevents the oxidation of the contents but also suppresses the formation of peroxides due to ultraviolet rays, etc., and prevents the skin aging phenomenon including wrinkles. Not only can be obtained, but also has a melanin synthesis inhibitory effect, so that it can have a whitening effect on stains, freckles and dullness. Therefore, the form applied directly to the skin is preferable, and skin cosmetics are particularly preferable. still. In the present invention, the topical skin preparation includes all those applied to the skin on the body including the scalp, and includes, for example, bathing agents without distinction between cosmetics, pharmaceuticals, quasi drugs and the like. Is.

  Examples will be described below. Moreover,% shown in an Example means the mass%. In addition, this invention is not limited to a following example. Each extract was a lyophilized solid. In addition, a short-cylinder hook ear grass was used as the hook ear grass.

Test Example 1 (Antioxidant power test)
0.05 mol / L sodium carbonate buffer (pH 10.2) 2.4 mL, 3 mmol / L xanthine 0.1 mL, 3 mmol / L EDTA 0.1 mL, 0.15% bovine serum albumin 0.1 mL, 0.75 mmol / L nitro Add 0.1 mL of 1.5 mg / mL water or 50% ethanol solution to 0.1 mL of blue tetrazolium and heat at 25 ° C. for 10 minutes. Thereto is added 0.1 mL of 250-fold diluted xanthine oxidase (manufactured by Sigma) derived from buttermilk and reacted at 25 ° C. for 20 minutes. 0.1 mL of 6 mmol / L cupric chloride was added, the reaction was stopped, and the absorbance was measured at a wavelength of 560 nm using a spectrophotometer. Instead of the sample solution, the inhibition rate (%) was obtained with the absorbance of 0.1 mL of water or 50% ethanol added as 100.

  As a result, as shown in Table 1, it was confirmed that the extract used in the present invention has an excellent antioxidant power.

Table 1 Antioxidant test results Sample Concentration Inhibition rate (%)
--------------------------------
Ear ear grass water extract 0.005% 91.7
(Aqueous solution)
Panicle 50% ethanol extract 0.005% 90.4
(50% ethanol solution)
Small rind water extract 0.005% 73.1
(Aqueous solution)
Small husk 50% ethanol extract 0.005% 68.6
(50% ethanol solution)

Test example 2 (melanin synthesis inhibition test)
(1) Melanin production inhibition test B16 melanoma cells were seeded in a 12-well plate at 2 × 10 ⁴ cells / well, and after 24 hours, the medium was replaced with a theophylline (Theophylline) -containing medium containing 10 μg / mL or 30 μg / mL of each sample compound. did. The cells were cultured for 72 hours, and then the cells were treated with 10% TCA, ethanol / diethyl ether (= 1/1). Subsequently, the OD475 value after dissolution in a 1 mol / L sodium hydroxide aqueous solution containing 10% dimethyl sulfoxide was determined and used as the amount of melanin. Thereafter, the number of cells was measured, and the inhibition rate (%) of melanin production per cell was determined.

  As a result, as shown in Table 2, it was confirmed that the extract used in the present invention has an excellent inhibitory effect on melanin synthesis.

Table 2 Results of melanin synthesis inhibition test Sample Concentration Inhibition rate
--------------------------------
Ear ear grass water extract 10μg / ml 20.9%
Panax 50% ethanol extract 10μg / ml 84.6%
Small husk extract 10 μg / ml 55.2%
30 μg / ml 73.3%
Small husk 50% ethanol extract 10 μg / ml 50.6%
30 μg / ml 76.3%

  From the above results, it was found that the extract of the present invention has an excellent antioxidant ability and further has an inhibitory effect on melanin synthesis.

Examples 1-8 (skin cream)
In the composition of Table 3, the extract was blended as described in Table 4, and a skin cream was prepared according to the following preparation method.

(Table 3)
Blending ingredients Blending amount (%)
(A) Oil phase Stearic acid 7.0
Oleic acid 3.0
Stearyl alcohol 4.0
Stearic acid monoglycerin ester 8.0
Extract (listed in Table 4)
Ethylparaben 0.1
Butylparaben 0.1
Propylparaben 0.1

(B) Water phase Propylene glycol 8.0
Glycerin 2.0
Potassium hydroxide 0.4
Edetate disodium 0.05
Purified water Remaining amount of 100

(Table 4)
Example 1 Example 2 Example 3 Example 4
Ear ear grass water extract 0.5% − − −
Ear ear grass water extract-0.1%--
Panicle grass 50% ethanol extract − − 0.5% −
Panacea 50% ethanol extract---0.1%

Example 5 Example 6 Example 7 Example 8
Small rind water extract 0.5% − − −
Small persimmon skin extract-0.1%--
Small husk 50% ethanol extract--0.5%-
Small husk 50% ethanol extract--0.1%

(Preparation method)
The oil phase part of A and the aqueous phase part of B were each heated to 70 ° C. and completely dissolved. Next, B phase was added to A phase, and it emulsified with the emulsifier. The emulsion was cooled and filled into a container to obtain a skin cream according to the present invention.

  The skin creams according to the present invention of Examples 1 to 8 did not show skin irritation and were confirmed to be excellent in stability over time.

Examples 9 to 20 (skin lotion)
In the composition of Table 5, the extract was blended as described in Table 6, and skin lotion was prepared according to the following preparation method.

(Table 5)
Blending ingredients Blending amount (%)
(A)
Ethanol 10.0
Polyoxyethylene monolaurate (6) sorbitan 5.0
Fragrance 0.05

(B)
Extract (listed in Table 6)

(C)
Glycerin 5.0
Xanthan gum 0.1
Hydroxyethyl cellulose 0.1
Purified water Remaining amount of 100

(Table 6)
Example 9 Example 10 Example 11
Ear ear grass water extract 0.1% − −
Ear ear grass water extract-1.0%-
Ear ear grass water extract − − 3.0%

Example 12 Example 13 Example 14
Ear plant grass 50% ethanol extract 0.1% − −
Panicle grass 50% ethanol extract-1.0%-
Panicle grass 50% ethanol extract--3.0%

Example 15 Example 16 Example 17
Small persimmon skin water extract 0.1% − −
Small persimmon water extract-1.0%-
Small persimmon skin water extract − − 3.0%

Example 18 Example 19 Example 20
Small husk 50% ethanol extract 0.1% − −
Small husk 50% ethanol extract-1.0%-
Small husk 50% ethanol extract--3.0%

(Preparation method)
After the B component was uniformly dissolved in the C component, the A component and the C component were mixed and stirred and dispersed uniformly, and then filled into a container to obtain a skin lotion according to the present invention.

  It was confirmed that the skin lotions according to the present invention of Examples 9 to 20 did not show skin irritation and were excellent in stability over time.

Examples 21-32 (lipstick)
In the composition of Table 7, the extract was blended as described in Table 8, and lipstick was prepared according to the following preparation method.

(Table 7)
Blending ingredients Blending amount (%)
(A)
Paraffin 16.0
Polybutene 10.0
Vaseline 20.0
Octyldodecanol 10.0
Liquid paraffin

(B)
Red No. 201 1.0
Red No. 202 0.5
Yellow No. 4 aluminum lake 0.3
Black iron oxide 0.1

(C)
Extract (described in Table 8)

(Table 8)
Example 21 Example 22 Example 23
Ear ear grass water extract 0.01% − −
Ear ear grass water extract-0.3%-
Ear ear grass water extract − − 3.0%

Example 24 Example 25 Example 26
兎 ear grass 50% ethanol extract 0.01% − −
兎 eargrass 50% ethanol extract − 0.3% −
Panicle grass 50% ethanol extract--3.0%

Example 27 Example 28 Example 29
Small persimmon skin extract 0.01% − −
Small persimmon skin extract-0.3%-
Small persimmon skin water extract − − 3.0%

Example 30 Example 31 Example 32
Small husk 50% ethanol extract 0.01% − −
Small husk 50% ethanol extract-0.3%-
Small husk 50% ethanol extract--3.0%

(Preparation method)
The A component was uniformly dissolved at 90 ° C., and the B component was added and kneaded. It melt | dissolved again at 90 degreeC, and deaerated, Then, C component was added, it poured into the metal mold | die, it cooled and formed, and it put in the container, and obtained the lipstick based on this invention.

  It was confirmed that the lipstick according to the present invention of Examples 21 to 32 did not show skin irritation and was excellent in stability over time.

Claims (2)

An anti-oxidant comprising an extract of eel ear grass or small scabbard. A skin external preparation characterized by containing an extract of eel ear grass or small scabbard.