JP2008279195A - Blood separation filter device - Google Patents

Abstract

A blood separation filter device capable of easily providing not only plasma or serum but also a whole blood sample and capable of providing both plasma or serum and whole blood with a single blood separation filter device is provided.
SOLUTION: A blood cell is introduced into a part of a flow path of a flow path forming member 3 having a first opening 3a and a second opening 3b, and a flow path through which blood flows is formed. A blood separation filter device 1 comprising a blood separation filter member 5 that separates components into plasma or serum, and a whole blood storage member 6 for storing the derived whole blood.
[Selection] Figure 1

Description

  The present invention relates to a blood separation filter device provided with a blood separation filter member for separating blood into blood cells and plasma or serum, and more particularly, only plasma or serum can be separated from blood in a single time. In addition, the present invention relates to a blood separation filter device that makes it possible to collect whole blood necessary for, for example, a blood cell test.

Conventionally, blood separation filter devices for separating blood into blood cell components and plasma or serum have been widely used in clinical examinations and the like. For example, Patent Document 1 below discloses an example of this type of blood separation filter device. Here, a flow path forming member having a flow path through which blood flows is provided. A blood separation filter member that separates blood into blood cell components and plasma or serum is disposed in the flow path of the flow path forming member. Further, in the flow path, a blood cell stop filter member for preventing the mixing of blood cell components is disposed on the downstream side of the blood separation filter member. In addition, a flow path closing member made of a water-swellable polymer that swells when contacting with plasma or serum and closes the flow path is disposed downstream of the blood separation filter member.
Japanese Patent No. 3809457

  When the blood separation filter device described in Patent Document 1 is used, plasma and serum can be reliably separated from various bloods having different hematocrit values and viscosities without requiring a complicated centrifugation operation. it can. Even if the erythrocytes captured by the blood separation filter member or the blood cell stop filter member are destroyed and hemolysis occurs after the separation is completed, the flow path is blocked by the water-swellable polymer. Ingredients in red blood cells generated by this do not get mixed into plasma or serum.

  However, in the blood separation filter device described in Patent Document 1, as described above, plasma or serum can be separated from blood and collected as a specimen, but a whole blood sample could not be taken out simultaneously. . Therefore, when a conventional blood separation filter device as described in Patent Document 1 is used, in order to obtain a whole blood sample necessary for clinical examination, in addition to the blood separation filter device, blood for obtaining a separate whole blood sample A sampling container had to be prepared and the operation had to be complicated.

  The object of the present invention is to provide not only plasma or serum, but also a whole blood sample necessary for blood glucose level and blood cell inspection, etc. It is an object of the present invention to provide a blood separation filter device that can provide both plasma or serum and a whole blood sample.

  A blood separation filter device according to the present invention includes a flow path forming member having a first opening that is an inlet of blood and a second opening that is an outlet of blood, and in which a flow path through which blood flows is formed A blood separation filter member that is disposed in a part of the flow path of the flow path forming member and separates the introduced blood into a blood cell component and plasma or serum, and one of the flow paths of the flow path forming member And a whole blood storage member for storing the guided whole blood.

  In the blood separation filter device according to the present invention, preferably, the whole blood storage member is disposed on the upstream side of the blood separation filter member. In this case, a part of the blood guided to the flow path of the flow path forming member is first stored by the whole blood storage member, and the remaining blood is guided to the blood separation filter member side on the downstream side. Serum will be separated. Therefore, the clean whole blood before contacting the blood separation filter member can be accommodated in the whole blood accommodating member.

  In the blood separation filter device according to the present invention, the blood storage member may be disposed on the side of the blood separation filter member so that the blood separation filter member and the whole blood storage member satisfy the transverse section of the flow path. . Also in this case, clean whole blood can be collected in the whole blood storage member, apart from the blood led to the blood separation filter member, among the blood led to the flow path. Then, plasma or serum can be separated from clean whole blood that has not passed or reached the blood separation filter member.

  In the blood separation filter device according to the present invention, preferably, the whole blood storage member is detachably disposed in the flow path of the flow path forming member. Therefore, the whole blood as a specimen can be easily provided by taking out the whole blood storage member in which the whole blood is stored in the flow path of the flow path forming member.

  In the blood separation filter device according to the present invention, preferably, an anticoagulant is accommodated in the whole blood accommodating member, and in this case, blood coagulation in the whole blood accommodating member can be reliably prevented.

  In the blood separation filter device according to the present invention, preferably, a blood cell stop filter member is provided downstream of the blood separation filter member, and the blood cell stop filter member prevents passage of blood cell components. Therefore, contamination of blood cells into the separated plasma or serum can be prevented.

  The blood separation filter device according to the present invention preferably further includes a bottomed tubular container having an opening at one end and a bottom at the other end, and the first opening of the flow path forming member is tubular. The flow path forming member is accommodated in the tubular container so as to be on the opening side of the container, the first opening of the flow path forming member and the opening of the tubular container are hermetically sealed by a stopper, and The inside is depressurized. In this case, it is possible to quickly guide blood from the outside of the blood separation filter device to the flow path of the flow path forming member using the internal pressure reduction, and to perform separation in the blood separation filter member. The plasma or serum easily separated into the blood separation filter member is collected at the bottom of the bottomed tubular container, and the whole blood is stored in the whole blood storage member. Therefore, after separation, by removing the flow path forming member from the tubular container, the whole blood stored in the whole blood storing member can be easily provided as a specimen, and the plasma collected at the bottom of the tubular container Alternatively, serum can be easily provided as a specimen as well.

  In the blood separation filter device according to the present invention, not only the blood separation filter member but also the whole blood storage member is disposed in the flow path of the flow path forming member, so that the blood separation filter member is separated by the action of the blood separation filter member It is possible to provide not only plasma or serum but also whole blood contained in the whole blood containing member as a specimen. Therefore, it is possible to provide not only plasma or serum but also whole blood as a specimen only by preparing one blood separation filter device. Therefore, when it is necessary to prepare plasma or serum and whole blood as samples, the number of instruments to be prepared can be reduced, and the work of preparing plasma or serum as whole samples as samples is simplified. can do. Therefore, it is possible to significantly reduce the cost of blood tests and reduce the burden on the work of the test staff.

  Hereinafter, the present invention will be clarified by describing specific embodiments of the present invention with reference to the drawings.

  FIG. 1 is a front sectional view showing a blood separation filter device according to a first embodiment of the present invention.

  The blood separation filter device 1 has a bottomed tubular container 2. The tubular container 2 is made of a material having shape retaining property such as an appropriate synthetic resin or glass described later, and is preferably made of a transparent material so that the inside can be observed.

  The tubular container 2 has an opening 2a at one end, and the other end is closed. The closed part at the other end, that is, the bottom part, constitutes a specimen storage part for storing plasma or serum.

  A flow path forming member 3 is inserted into the tubular container 2. In this embodiment, the flow path forming member 3 has a cylindrical shape, and has a first opening 3a on the upper side. The first opening 3a constitutes an inlet on the side through which blood is introduced. A second opening 3b is provided at the other end. The opening 3b is an outlet for the separated plasma or serum. In the present embodiment, the second opening 3b is a hollow flow that penetrates in the vertical direction in the protruding portion 3d that protrudes downward from the center of the bottom plate portion 3c provided in the lower portion of the flow path forming member 3. It is formed by the lower end of the path. That is, the portion where the hollow channel is open at the lower end is the second opening 3b.

  Similarly to the tubular container 2, the flow path forming member 3 is made of a material having shape retention such as an appropriate synthetic resin or glass. In this embodiment, it consists of a synthetic resin molded product. However, the flow path forming member 3 is not limited to a synthetic resin molded product, and may be formed by joining a plurality of members.

  In the present embodiment, the flow path forming member 3 has a circular cross section. And the plug body 4 is attached so that the 1st opening 3a and the opening 2a of the tubular container 2 may be airtightly sealed. The plug 4 is made of an elastic material such as rubber or elastomer. The plug 4 includes a large diameter portion 4a, a medium diameter portion 4b having a smaller diameter than the large diameter portion 4a, a small diameter portion 4c having a smaller diameter than the medium diameter portion 4b, and a tip having a smaller diameter than the small diameter portion 4c. Part 4d. The medium diameter part 4b is press-fitted from the opening 2a side of the tubular container 2, the inside of the tubular container 2 is hermetically sealed, and the small diameter part 4c is press-fitted from the opening 3a side of the flow path forming member 3, so that the flow path forming member 3 opening 3a is hermetically sealed.

  On the other hand, the flow path forming member 3 forms a flow path through which blood flows between the first opening 3a and the second opening 3b. In this flow path, the blood separation filter member 5 is disposed so as to occupy the entire area of the cross section in a part of the flow path. A whole blood storage member 6 is disposed on the upstream side of the blood separation filter member 5. Further, a blood cell stop filter member 7 is disposed on the downstream side of the blood separation filter member.

  The blood separation filter member 5 is made of an appropriate filter material that acts to separate the introduced blood into a blood cell component and plasma or serum due to a difference in moving speed. The material constituting the blood separation filter member 5 will be described later.

  In the present embodiment, the whole blood storage member 6 is disposed on the upstream side of the blood separation filter member 5 with a predetermined gap from the upstream end portion 5a of the blood separation filter member 5. In the present embodiment, the whole blood containing member 6 has a cylindrical portion 6b having an opening 6a opened upward. The cylindrical portion 6 has a substantially cylindrical shape, and the distal end portion 4d of the plug body 4 is press-fitted into the opening 6a, and the opening 6a is hermetically sealed.

  Accordingly, the whole blood storage member 6 is fixed and fixed to the plug body 4 by press-fitting the distal end portion 4d of the plug body 4 from the opening 6a side.

  A bottom plate portion 6c is provided at the lower end of the cylindrical portion 6b. At the center of the bottom plate portion 6c, a cylindrical protruding portion 6d is formed so as to protrude toward the inside of the cylindrical portion 6b, that is, upward. The cylindrical protrusion 6d has a hollow through channel 6e. The hollow through channel 6e penetrates the cylindrical protrusion 6d.

  The cylindrical projecting portion 6d is provided so as to project upward at the center of the bottom plate portion 6c. Therefore, a donut-shaped space is formed between the cylindrical protruding portion 6d and the inner wall of the cylindrical portion 6b. This donut-shaped space is the whole blood accommodating part 6f.

  The whole blood containing member 6 is formed of an appropriate synthetic resin or glass described later.

  In the present embodiment, the lower surface of the bottom plate portion 6 c of the whole blood containing member 6 is disposed with a gap from the upper end 5 a of the blood separation filter member 5, but is in contact with the upstream end portion 5 a of the blood separation filter member 5. May be. However, in order to guide the guided blood over the entire region of the end portion 5a at the end portion 5a of the blood separation filter member 5, the end portion 5a is spaced from the lower surface of the bottom plate portion 6c as in the present embodiment. It is desirable that

  Moreover, although the whole blood accommodating member 6 has the one cylindrical protrusion part 6d, it is good also as a plurality of cylindrical protrusion parts 6d, and making the space of each cylindrical protrusion part the whole blood accommodating part.

  Similarly to the blood cell stop filter member described in Patent Document 1, the blood cell stop filter member 7 is made of an appropriate filter material that prevents passage of blood cell components such as red blood cells. In this embodiment, the blood cell stop filter member 7 has a flat sheet shape and is disposed in contact with the lower surface of the blood separation filter member 5. The blood separation filter member 5 and the blood cell stop filter 7 are not necessarily in contact with each other, and a gap may be provided. Further, the lower surface of the blood cell stop filter 7 is in contact with the bottom plate portion 3c. That is, the blood cell stop filter member 7 and the blood separation filter member 5 are inserted into the flow path forming member 3 in this order and arranged on the bottom plate portion 3c. However, if the lower surface of the blood cell stop filter 7 is completely in close contact with the bottom plate portion 3c, the flowability of serum or plasma is deteriorated. Therefore, it is preferable to provide an appropriate gap such as providing irregularities on the bottom plate portion.

  Next, the material which comprises each member of the said blood separation filter apparatus 1 is demonstrated.

  As described above, the tubular container 2, the flow path forming member 3, and the whole blood containing member 6 can be formed of an appropriate synthetic resin or glass. Examples of such materials include polyethylene, polypropylene, polystyrene, polyethylene terephthalate, polymethyl methacrylate, polyacrylonitrile, polyamide, acrylonitrile-styrene copolymer, ethylene-vinyl alcohol copolymer, and other thermoplastic resins, and unsaturated polyester. Thermosetting resins such as resins, epoxy resins or epoxy-acrylate resins, modified natural resins such as cellulose acetate, cellulose propionate, ethyl cellulose, ethyl chitin, and silicate glasses such as soda lime glass, phosphosilicate glass or borosilicate glass Alternatively, conventionally known materials such as glass such as quartz glass and those containing one of these as a main component, or a combination of two or more of these may be used.

  The elastic material constituting the plug body 4 is not particularly limited as long as the inside can be hermetically sealed, and examples thereof include various rubbers such as natural rubber and synthetic rubber, and thermoplastic elastomers. The outer surface of the elastic material may be covered with a metal foil, such as an aluminum foil, or may be covered with an aluminum vapor deposition sheet or the like.

  As described above, the blood separation filter member 5 is made of an appropriate material that separates blood cell components and plasma or serum from blood due to a difference in moving speed. Examples of the material constituting the blood separation filter member 5 include polypropylene, polyethylene, polyethylene terephthalate, polybutylene terephthalate, polystyrene, polyvinyl alcohol, urethane, acrylic, rayon, and glass.

  Examples of the blood separation filter member 5 include, for example, a fiber in which the above-described material is formed and accumulated in a nonwoven fabric, a molded body such as an open-cell foam in which continuous pores are formed using the above-described material, and an approximately molded material. One in which spherical fine particles are accumulated so as to have a finely packed structure, or one in which this accumulated material is integrally molded by sintering, one in which a through hole is formed in a film formed from the above material, or one side of the film In addition, there may be mentioned a laminate in which a large number of sheets processed with corona discharge or pressing are laminated on both sides.

  The average pore diameter of the blood separation filter member 5 is preferably in the range of 2 to 10 μm in order to enable separation of blood cell components from plasma or serum due to a difference in moving speed and to prevent damage to blood cell components, that is, destruction of red blood cells. More preferably, it is the range of 3-8 micrometers. If the average pore size is smaller than 2 μm, blood cells are clogged in the blood separation filter member 5 and filtration is hindered, or resistance to blood cell components is increased during filtration and red blood cells are easily destroyed. When the average pore diameter exceeds 10 μm, the difference in moving speed between the blood cell component and plasma or serum becomes small, and the blood cell component easily reaches the flow path blocking member in a short time. The average pore diameter is measured by a bubble point test method (JIS K 3832), an actual measurement method using an enlarged image by an electron microscope, or the like.

  The porosity of the blood separation filter member 5 is preferably in the range of 20 to 97%, more preferably in the range of 30 to 95%, in order to ensure a practical filtration time. If the porosity is lower than 20%, it takes a long time for filtration and the destruction of red blood cells tends to occur. When the porosity is higher than 97%, the shape retention of the blood separation filter member 5 is lowered, and the blood separation filter member 5 may be deformed by the pressure during filtration and the pore diameter may be changed, so that blood separation is stable. May not be done.

  As a material constituting the blood cell stop filter member 7, an appropriate filter material capable of passing red blood cells can be used. Such a material is not particularly limited. For example, polyvinylidene difluoride, polytetrafluoroethylene, cellulose acetate, nitrocellulose, polycarbonate, polyethylene terephthalate, polyethylene, polypropylene, glass fiber, borosilicate, vinyl chloride or silver. Etc.

  When the blood cell stop filter member 5 is made of a porous material, plasma or serum can pass through. The porous substance constituting the blood cell stop filter member 5 is not particularly limited as long as it has a pore diameter within a range that can prevent passage of red blood cells. In order to prevent passage of red blood cells, the pore diameter is preferably 2 μm or less. If the pore size is small, clogging may occur due to protein components in the blood. Therefore, the pore size is preferably 0.05 μm or more. In order to effectively prevent passage of red blood cells, the pore diameter is more preferably in the range of 0.1 to 1.5 μm.

  In order to increase the flow rate of filtration, the surface of the blood cell stop filter member 5 may be subjected to a hydrophilic treatment. Examples of the hydrophilic treatment method include plasma treatment and coating with a hydrophilic polymer, but are not limited to these methods, and other methods may be used.

  The blood separation filter device 1 is preferably depressurized, and the internal pressure is about 5 to 90 kPa. Therefore, as will be apparent from the usage method described later, blood can be quickly guided into the blood separation filter device 1 by the vacuum blood collection method and filtered by the blood separation filter member 5. If the internal pressure is lower than 5 kPa, the pressure difference becomes too large, the red blood cells may be destroyed, and the guided blood may be hemolyzed. If the internal pressure exceeds 90 kPa, filtration by the pressure difference may not be performed quickly. is there.

  It is preferable that an anticoagulant is stored in the whole blood storage portion 6f of the whole blood storage member 6. Although it does not specifically limit as an anticoagulant, Well-known anticoagulants, such as a heparin salt and ethylenediaminetetraacetate, can be used. There is no particular limitation on the form in which such an anticoagulant is accommodated, and the anticoagulant is sprayed on the whole blood accommodating part of the whole blood accommodating member 6 or attached to the surface of the whole blood accommodating member 6. It is possible to use a method of leaving them. When a whole blood sample required for blood cell examination is required, it is desirable to contain ethylenediaminetetraacetate as an anticoagulant, but an appropriate anticoagulant should be used depending on the test content using the whole blood sample. Use it.

  In the present embodiment, the whole blood containing member 6 is fixed to the plug body 4 and integrated as described above, but is detachably disposed with respect to the flow path forming member 3. Therefore, according to the blood separation filter device 1 of the present embodiment, plasma or serum and whole blood can be easily collected from blood as samples. This will be described with reference to the usage method of FIG.

  As shown in FIG. 2A, the hollow needle 8 is pierced through the plug body 4 to guide the blood into the flow path forming member 3 of the blood separation filter device 1. The hollow needle 8 may be a hollow needle positioned on the side opposite to the hollow needle inserted into the blood vessel of the vacuum blood collection needle, or may be a hollow needle attached to a syringe or the like. That is, the blood collected in the syringe may be guided from the hollow needle 8 to the blood separation filter device 1, or the blood may be directly guided into the flow path forming member 3 by a vacuum blood sampling method. The blood is guided from the side of the opening 3a that is the blood inlet of the flow path forming member 3. In this case, as shown in FIG. 2B, when the hollow needle 8 is positioned above the through channel of the cylindrical protrusion 6d, blood first flows downward through the through channel 6e. The blood separation filter member 5 is guided onto the end portion 5a.

  As shown in FIG. 2 (b), when more blood is introduced from the hollow needle 8, the blood is also stored in the whole blood storage portion 6 f of the whole blood storage member 6. After the necessary amount of blood has flowed in, the hollow needle 8 is once removed, and another hollow needle 8 is pierced to bring the space between the plug 4 and the upstream end 5a to atmospheric pressure. . By this operation, a pressure difference is generated between the upstream side and the downstream side of the blood separation filter 5, and filtration proceeds.

  As a result, as shown in FIG. 2C, plasma or serum A passes through the blood separation filter member 5 and the blood cell stop filter member 7 and is collected downward. On the other hand, the whole blood sample is accommodated in the whole blood accommodating member 6. When the filtration is completed as shown in FIG. 3A, plasma or serum A is stored in the specimen storage portion of the lower tubular container 2, and whole blood B is stored in the whole blood storage portion 6f of the upper whole blood storage member 6. Will be housed.

  Thereafter, the hollow needle 8 is pulled out, and then the middle diameter portion 4b of the plug body 4 is pulled out from the opening 2a of the tubular container 2 as shown in FIG. 3B, and the flow path forming member 3 is removed together with the plug body 4. Thereafter, as shown in FIG. 4A, the small diameter portion 4 c of the plug body 4 is pulled out from the first opening 3 a of the flow path forming member 3. As a result, the whole blood containing member 6 integrated with the plug 4 is separated in a state integrated with the plug 4. Therefore, by removing the plug 4 from the opening 6a of the whole blood storage member 6, the whole blood storage member 6 in which the whole blood B is stored in the whole blood storage portion 6f is removed as shown in FIG. Obtainable. Therefore, whole blood can be easily taken out from the opening 6a of the whole blood containing member 6 and can be provided for blood cell examinations and the like. On the other hand, since plasma or serum A is stored in the specimen storage section below the tubular container 2 as shown in FIG. 3B, plasma or serum A can also be easily taken out as an analysis sample. it can.

  FIG. 5A is a front sectional view of the blood separation filter device 11 according to the second embodiment of the present invention, and FIG. 5B is a sectional view taken along line XX in FIG. The blood separation filter device 11 has a tubular container 12 having the same structure as the tubular container 2 prepared in the first embodiment. A plug 14 is press-fitted into the opening 12 a of the tubular container 12. The plug body 14 is made of an elastic material similar to that of the plug body 4 of the first embodiment. The plug 14 includes a large diameter portion 14a, a medium diameter portion 14b having a smaller diameter than the large diameter portion 14a, and a small diameter portion 14c having a smaller diameter than the medium diameter portion 14b.

  The medium diameter portion 14b is press-fitted into the opening 12a of the tubular container 12, and the opening 12a side of the tubular container 12 is hermetically sealed. In addition, the flow path forming member 13 has a first opening 13a on the upper side, similarly to the flow path forming member 3 of the first embodiment. The small diameter portion 14c of the plug 14 is press-fitted into the opening 13a, and the first opening 13a side is hermetically sealed.

  The flow path forming member 13 is formed of a cylindrical member similarly to the flow path forming member 3 used in the first embodiment, has the first opening 13a above, and has a bottom plate portion 13c at the lower end. . A protruding portion 13d is provided so as to protrude downward from the center of the bottom plate portion 13c. The protruding portion 13d is provided with a hollow channel penetrating in the vertical direction, and a lower end opening of the hollow channel is provided. Is the second opening 13b. Up to this point, the flow path forming member 13 is formed in the same manner as the flow path forming member 3. The difference is that in the flow path forming member 13, a partition wall 13e is formed so as to extend upstream from the bottom plate portion 13c. The partition wall 13e extends from the upper surface of the bottom plate portion 13c to the upstream side, but is formed so as not to reach the lower surface of the small diameter portion 14c of the plug body 14. The partition wall 13e divides the flow path into a space on one side and a space on the other side of the partition wall 13e. And the blood separation filter member 15 is arrange | positioned in the space of one side, and the whole blood accommodating member 16 is arrange | positioned in the space of the other side. That is, the whole blood storage member 16 is arranged on the side of the blood separation filter member. In the flow path portion where the partition wall 13e is provided, the blood separation filter member 15 and the whole blood storage member 16 are formed so as to occupy the entire region viewed in cross section. That is, in the space on one side of the partition wall 13e, all of the guided blood is guided to the upstream end 15a side of the blood separation filter member 15, and in the other side space, the blood is guided into the whole blood containing member 16. It is configured to be. In other words, the blood is configured not to flow downward without passing through the whole blood storage member 16 and without passing through the blood separation filter member 15.

  The whole blood storage member 16 is detachably attached to the flow path forming member 13.

  The whole blood containing member 16 is made of a synthetic resin molded product, but is made of an appropriate material such as synthetic resin or glass.

  On the lower surface of the blood separation filter member 15, a blood cell stop filter member 17 is disposed.

  The material constituting the blood separation filter member 15 and the blood cell stop filter member 17 is the same as the material constituting the blood separation filter member 5 and the blood cell stop filter member 7 of the above-described embodiment. The material constituting the whole blood containing member 16 can also be configured in the same manner as the material constituting the whole blood containing member 6 of the above-described embodiment. In the present embodiment, the whole blood containing member 16 is formed of a bottomed cylindrical body having an opening 16a on the upper side. Accordingly, whole blood is guided from the opening 16a side, and the whole blood is accommodated therein.

  The usage method of the blood separation filter apparatus 11 of this embodiment is demonstrated with reference to FIGS. First, as shown in FIG. 6A, similarly to the case of FIG. 2A, the hollow needle 8 is pierced through the plug 14 to introduce blood into the interior. A part of the blood is immediately introduced into the whole blood containing member 16 from the opening 16a of the whole blood containing member 16 and collected.

  The inside of the blood separation filter device 11 is decompressed to about 5 to 90 kPa. Therefore, the blood guided to the upper surface of the blood separation filter member 15 is filtered by the pressure difference. As a result, as shown in FIGS. 6 (b) and 6 (c), the filtration proceeds, and plasma or serum A is collected in the specimen storage section at the bottom of the tubular container 2.

  Thereafter, as shown in FIG. 7, the flow path forming member 13 can be removed together with the plug body 4 by pulling out the medium diameter portion 14 b of the plug body 14 from the tubular container 12. Therefore, plasma or serum A in the tubular container 2 can be immediately provided as a specimen.

  After that, the plug body 14 is pulled out from the flow path forming member. As a result, the opening 16a of the flow path forming member 16 is opened as shown in FIG. Thereafter, the whole whole blood accommodating member 16 may be pulled out. As a result, as shown in FIG. 9, the whole blood B accommodated in the whole blood accommodating member 16 can be provided as a specimen.

It is front sectional drawing of the blood separation filter apparatus which concerns on the 1st Embodiment of this invention. (A)-(c) is each front sectional drawing for demonstrating the usage method of the blood separation filter apparatus of 1st Embodiment. (A), (b) is each front sectional drawing for demonstrating the usage method of the blood separation filter apparatus of 1st Embodiment. (A), (b) is each front sectional drawing for demonstrating the usage method of the blood separation filter apparatus of 1st Embodiment. It is front sectional drawing of the blood separation filter apparatus which concerns on 2nd Embodiment of (a), (b), and sectional drawing which follows the XX line in (a). (A)-(c) is each front sectional drawing for demonstrating the usage method of the blood separation filter apparatus of 2nd Embodiment. It is front sectional drawing for demonstrating the usage method of the blood separation filter apparatus of 2nd Embodiment. It is front sectional drawing for demonstrating the usage method of the blood separation filter apparatus of 2nd Embodiment. It is front sectional drawing for demonstrating the usage method of the blood separation filter apparatus of 2nd Embodiment.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Blood separation filter apparatus 2 ... Tubular container 2a ... Opening 3 ... Channel formation member 3a, 3b ... 1st, 2nd opening 3c ... Bottom plate part 3d ... Projection part 4 ... Plug body 4a ... Large diameter part 4b ... Medium diameter Part 4c: Small diameter part 4d ... Tip part 5 ... Blood separation filter member 5a ... Upstream end part 6 ... Whole blood containing member 6a ... Opening 6b ... Cylindrical part 6c ... Bottom plate part 6d ... Cylindrical protrusion 6e ... Hollow through flow Path 6f ... Whole blood storage member 7 ... Blood cell stop filter member 8 ... Hollow needle 11 ... Blood separation filter device 12 ... Tubular container 12a ... Opening 13 ... Flow path forming member 13a ... First opening 13b ... Second opening 13c ... Bottom plate part 13d ... Projection part 13e ... Partition wall 14 ... Plug 14a ... Large diameter part 14b ... Medium diameter part 14c ... Small diameter part 15 ... Blood separation filter member 15a ... End part 16 ... Whole blood containing member 16a ... Opening 17 ... Blood cell Stop filter member ... plasma or serum B ... whole blood

Claims (7)

A flow path forming member having a first opening that is an inlet of blood and a second opening that is an outlet of blood, and in which a flow path through which blood flows is formed;
A blood separation filter member that is disposed in a part of the flow path of the flow path forming member and separates the guided blood into a blood cell component and plasma or serum;
A blood separation filter device, comprising: a whole blood storage member that is disposed in a part of the flow path of the flow path forming member and stores the guided whole blood.   The blood separation filter device according to claim 1, wherein the whole blood containing member is disposed upstream of the blood separation filter device in the flow path.   In the flow path of the flow path forming member, the whole blood storage member is disposed on the side of the blood separation filter member so that the blood separation filter member and the whole blood storage member fill a cross section of the flow path. The blood separation filter device according to claim 1.   The blood separation filter device according to any one of claims 1 to 3, wherein the whole blood storage member is disposed in the flow path so as to be removable from the flow path forming member.   The blood separation filter device according to any one of claims 1 to 4, wherein an anticoagulant is contained in the whole blood containing member.   The blood separation filter device according to any one of claims 1 to 5, further comprising a blood cell stop filter member that is disposed downstream of the blood separation filter member and prevents passage of blood cell components. It further includes a bottomed tubular container having an opening at one end and a bottom closed at the other end, and the flow path forming member is configured such that the first opening is on the opening side of the tubular container. A path forming member is accommodated in the tubular container;
The blood separation filter device further comprising a stopper for hermetically sealing the first opening of the flow path forming member and the opening of the tubular container, and the inside is decompressed.

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