JP2008019170A - Food and drink having ameliorating and prophylactic action on gastrointestinal injury - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a food and drink available from an inexpensive material, administrable over a long period and effectively exhibiting ameliorating and/or prophylactic actions on gastrointestinal injury without adverse effects. <P>SOLUTION: The food and drink comprises a composition obtained from an asparagus, preferably an extract from a young stem of the asparagus and one or two or more substances selected from the group consisting of methylmethionine and/or a derivative thereof, niacin and/or a derivative thereof, vitamin B2, an aloe, mucin and a lactic acid bacterium and has the ameliorating and/or prophylactic actions. The composition obtained from the asparagus contains 1-40 mass% of γ-aminobutyric acid. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a food or drink having a gastrointestinal disorder improving action and / or a gastrointestinal disorder preventing action.

  Gastrointestinal disorders such as gastric ulcer, duodenal ulcer, gastric cancer, and colon cancer are increasing due to the recent high stress society and westernization of eating habits. In general, gastric ulcer is said to be caused by gastric fluid stimulation when the function of the gastric mucosa is weakened, and duodenal ulcer is said to be caused by gastric juice becoming too strong. If these ulcers are left untreated, they will not only continue to be painful but also develop into serious situations such as the appearance of malignant tumors, so it is necessary to improve them early and prevent them on a daily basis. Among these, antacids such as sodium hydrogen carbonate, calcium carbonate, magnesium oxide, anticholinergic agents such as propantheline bromide, methylbenactidium bromide, butylscopolamine bromide, butropium bromide, isopropamide iodide, Various drugs for improving gastrointestinal disorders have been developed and used, such as anti-gastrin agents such as proglumide and oxesasein, H2 blockers such as cimetidine, ranitidine, famotidine, and roxatidine, and proton pump inhibitors such as omeprazole and lansoprazole.

  In addition, from the viewpoint that it is preferable to improve and / or prevent gastrointestinal disorders by paying attention to daily dietary habits, gastrointestinal disorders are also improved and / or prevented from among ingredients contained in food materials and food ingredients. Things to be found have been found. Examples thereof include methylmethionine and / or its derivatives (vitamin U), niacin and / or its derivatives, vitamin B2, aloe, mucin, lactic acid bacteria, and the like, which are abundant in cabbage and asparagus.

On the other hand, asparagus is a vegetable that is rich in fiber and low in calories, and about 28,000 tons are harvested annually throughout the country, and imports from overseas are also popular. Prefectures with high yields in Japan include Nagano Prefecture, Hokkaido, Saga Prefecture, Fukushima Prefecture, Kagawa Prefecture, Nagasaki Prefecture, and Akita Prefecture. Until now, it has been known that asparagus contains a lot of useful components such as aspartic acid, vitamin U (methylmethionine), rutin, folic acid, saponins, glutathione in addition to dietary fiber. (See Patent Document 1) and the like have been reported. It has also been reported that γ-aminobutyric acid (hereinafter abbreviated as GABA) is contained in a relatively large amount.
Japanese Patent Laid-Open No. 5-271405

  However, in the above-mentioned pharmaceuticals for the purpose of improving and / or preventing gastrointestinal disorders, antacids are only pharmaceuticals that neutralize gastric acid, and other pharmaceuticals have problems in which side effects are unavoidable. Taking was not preferred. In addition, various food materials such as those described above that have no side effects and have an action to improve gastrointestinal disorders and prevent gastrointestinal disorders have been developed, but none of them are necessarily satisfactory.

  An object of the present invention is to provide a food and drink that is obtained from an inexpensive material, has no side effects, can be taken for a long time, and can effectively exhibit an action for improving and / or preventing gastrointestinal disorders.

  As a result of intensive studies to solve the above problems, the present inventors have added a composition obtained from asparagus to a specific substance that has been found to have an effect of improving and / or preventing gastrointestinal disorders. As a result, it was found that a strong gastrointestinal disorder improving action and / or gastrointestinal disorder preventing action was obtained, and the present invention was achieved.

  That is, the present invention relates to a composition obtained from asparagus, and one or more substances selected from the group consisting of methylmethionine and / or its derivatives, niacin and / or its derivatives, vitamin B2, aloe, mucin and lactic acid bacteria. And a food and drink having an action for improving / preventing gastrointestinal disorders, wherein the composition obtained from asparagus is preferably an extract from asparagus young shoots More preferably, the composition obtained from asparagus is a composition containing 1% by mass to 40% by mass of γ-aminobutyric acid.

  According to the present invention, it is possible to obtain a gastrointestinal disorder improving action and / or a gastrointestinal disorder preventing action safely and effectively, and synergistically enhancing the degree of the conventionally known gastrointestinal disorder improving action and / or gastrointestinal disorder preventing action. It was possible to make it.

  The asparagus used in the present invention may be any as long as it does not impair the effects of the present invention. Asparagus is green asparagus grown under sunlight, white asparagus cultivated while shading with soil, etc., mini asparagus harvested while being thin and short, purple asparagus that is a different species from green asparagus and purple. Among them, green asparagus and murasakia paragas rich in nutrient components such as amino acids and rutin are preferable, and green asparagus is more preferable because of its low cost.

  The production area of asparagus is not particularly limited, and it may be domestically produced or imported from overseas. The part to be used is not particularly limited, and young stems, above-ground stems, and storage roots can be used. Among these, young stems are preferable. The young stem may be the root portion or the tip portion, but the root portion cut when aligning the length of the asparagus as the product is most preferable because it can be obtained at low cost. Asparagus may be used as it is, or may be used after processing such as crushing, cutting, freeze-drying and dehydration.

  The composition obtained from asparagus used in the present invention is a processed product obtained by subjecting asparagus to the known treatment as described below, and any composition may be used as long as the effects of the present invention are not impaired. . Such treatment includes, for example, treatment of any one or a combination of two or more known techniques such as pulverization, cutting, heating, cooling, freezing, dehydration, freeze drying, vacuum drying, spray drying, and water extraction. , Solvent extraction, pressing, enzymatic degradation, supercritical extraction, concentration, dilution, solid-liquid separation, purification, and other known techniques can be used alone or in combination. Some of these are described below.

  Crushing / chopping is a method of physically crushing asparagus finely, crushing by impact, and crushing by cutting. Grinding and shredding may be performed manually using a mortar, kitchen knife, cutter knife, scissors, or the like, but an apparatus is used when a large amount of asparagus is to be processed in a short time. Examples of such an apparatus include a mill, a hammer type pulverizer, a mixer, a blender, and the like, and a vegetable shredding machine may be used. The size of the crushed and shredded asparagus is not particularly limited, but is preferably 2 cm or less, and more preferably 5 mm or less.

  Dehydration is an operation for removing moisture in asparagus, and examples thereof include squeezing, filtration, separation of a precipitate by standing, centrifugation, heat evaporation, freeze drying, vacuum drying, spray drying, and the like. Of these, freeze-drying is preferred in that the possibility of component loss, decomposition, and alteration is low.

  Freeze drying and vacuum drying are methods in which the atmospheric pressure is lowered to lower the boiling point and vaporize water. Spray drying is an operation that enables drying in a short time by atomizing the solution and bringing it into contact with hot air.

  As a preferable example of the combination of the above treatments, it is preferable to heat-treat asparagus, freeze-dry, and then pulverize with a mill or the like.

  Water extraction is a method in which water is added to elute components. The amount of water to be added is not particularly limited, but is preferably 0.01 to 100 times, more preferably 0.5 to 5 times the amount of asparagus. When the amount of water is less than 0.01 times, the extraction efficiency is lowered, and when it is more than 100 times, only a thin extract can be obtained and a concentration operation may be required later. Moreover, 0 to 100 degreeC is preferable and the temperature of the water to be used has more preferable 10 to 80 degreeC. When the temperature of water is lower than 0 ° C., the extraction efficiency tends to decrease, and when the extraction temperature is higher than 100 ° C., active ingredients other than GABA may be decomposed.

  Solvent extraction is a method of extraction using an organic solvent such as alcohols, hydrocarbons and lipids, and the organic solvent used is not particularly limited as long as the effects of the present invention are not impaired, and may be used alone. In addition, it may be used by mixing with other solvents, or may be used by mixing with water. Examples of preferred organic solvents include ethanol, methanol, propanol, butanol, hexane, acetone, glycerin, propylene glycol, ethylene glycol, polyethylene glycol, propylene glycol fatty acid ester, polyethylene glycol fatty acid ester, ethyl ether, methyl ethyl ketone, dimethyl sulfoxide (DMSO ), N, N-dimethylformamide (DMF), N, N-dimethylacetamide (DMAC), and more preferably ethanol, hexane, acetone, DMSO, and glycerin. The amount of the organic solvent to be added is not particularly limited by the type of the organic solvent, but is preferably 0.01 to 100 times, more preferably 0.5 to 5 times the amount of asparagus. When the amount of the organic solvent is less than 0.01 times, the extraction efficiency is lowered, and when it is more than 100 times, only a thin extract can be obtained and a concentration operation may be required later. Moreover, the temperature of the organic solvent to be used is preferably -20 ° C to 200 ° C, more preferably 0 ° C to 120 ° C. When the temperature of the organic solvent is lower than −20 ° C., the extraction efficiency tends to decrease, and when the extraction temperature is higher than 200 ° C., the active ingredient may be decomposed.

  Squeezing is a method in which physical pressure is applied to asparagus to squeeze the liquid and transfer GABA to the juice. The pressure may be applied only in one direction, may be applied from two or more directions, and may be accompanied by a shearing force. Although the operation of pressing is easy if a commercially available pressing machine is used, it may be performed by a method that does not use a machine such as hand pressing or stepping. At this time, water or hot water may be added to asparagus and squeezed.

  Enzymatic decomposition is a method in which an enzyme is allowed to act on asparagus and then separated into solid and liquid to transfer GABA to a filtrate. Here, the enzyme is not particularly limited as long as the effect of the present invention is not impaired, but an enzyme that can be used for food is preferable in consideration of use for food. The type of enzyme is not particularly limited, but cellulase, hemicellulase, pectinase, xylanase, arabinase, arabanase, arabanase, amylase, glucanase, etc., in order to decompose asparagus fiber, pectin, polysaccharide, etc. In order for dextranase to degrade proteins and recover many free amino acids, protease, peptidase, etc. to convert glutamic acid to GABA, glutamic acid decarboxylase, etc. to convert glutamine to glutamic acid Glutaminase and the like can be used. In the enzymatic degradation in the present invention, only one kind of the above-described enzymes may be used, or two or more kinds may be used simultaneously or successively.

  In supercritical extraction, carbon dioxide or water is brought to a pressure and temperature above the gas-liquid critical point to make it a supercritical fluid with active molecular motion, and this is used as an extraction solvent. In the present invention, the supercritical fluid is preferably carbon dioxide. The temperature during supercritical extraction is preferably 31 ° C to 150 ° C, more preferably 31 ° C to 100 ° C. When the temperature is higher than this range, there is a possibility that useful components are decomposed. When the temperature is lower than this range, there is a problem that the extraction efficiency is lowered. Moreover, the pressure at the time of performing supercritical extraction is preferably 7 MPa to 50 MPa, and more preferably 7 MPa to 30 MPa. When the pressure is higher than this range, there is a problem in decomposition of useful components, high cost, and safety, and when the pressure is lower than this range, the extraction efficiency tends to decrease.

  Concentration is an operation to reduce the amount of water without reducing other components, and may be performed by any method such as vacuum concentration, heat concentration, concentration using a filtration membrane, but in the range of 20 ° C to 60 ° C. It is preferable to carry out concentration under reduced pressure.

  Solid-liquid separation is a method that separates the solvent and components dissolved in it from insoluble solids. For example, any method such as filter filtration, squeeze filtration, centrifugation, or decantation can be used. it can. In order to obtain a clear composition, it is preferable to perform filter filtration using a filter aid such as diatomaceous earth. Moreover, when obtaining a clearer liquid or removing microorganisms, it is preferable to further filter this with a membrane filter having a pore diameter of less than 1 μm.

  Purification is an operation that separates the target component from other components. Methods include electrophoretic separation, density gradient centrifugation, membrane separation such as reverse osmosis, ultrafiltration and dialysis, gel filtration, ion exchange, Examples include affinity chromatography. These purification methods may be any method as long as the effects of the present invention are not impaired, but chromatography using a strongly acidic cation exchange resin is desirable from the viewpoint of high yield and simplicity.

  Examples of the form of the composition obtained from the asparagus in the present invention obtained by the treatment as described above include aqueous solutions, creams, suspensions, gels, powders, tablets, capsules and the like. Among these, preferable examples include those obtained by concentrating or pulverizing an extract obtained by performing water extraction after cutting.

  Further, the composition obtained from asparagus used in the present invention includes those having an increased GABA content. The method for increasing the content of GABA is not particularly limited as long as it does not impair the effects of the present invention. GABA can be used by adding GABA that can be used in foods and drinks, or by using an endogenous enzyme of asparagus from glutamic acid and / or glutamate. And a method of converting glutamic acid and / or glutamate into GABA by a microorganism. Among these, a method of converting glutamic acid and / or glutamate which is a highly safe food additive into GABA by a microorganism is preferable. As such microorganisms, some such as lactic acid bacteria, yeast, and tempe bacteria are known as GABA-producing microorganisms, and lactic acid bacteria are particularly preferable. Specifically, glutamic acid and / or glutamic acid sodium salt is added to an aqueous extract obtained from asparagus in an amount of 0.1 to 20% by mass, lactic acid bacteria capable of producing GABA are added, and 25 to 35 ° C., The method of fermenting for 2-70 hours by maintaining pH 4.0-6.0 and enriching GABA is mentioned.

  The content of GABA contained in the composition obtained from asparagus used in the present invention is preferably 1 to 40% by mass, more preferably 1 to 20% by mass, and still more preferably 5 to 20% by mass. When GABA is less than 1% by mass, it is necessary to use a large amount of a composition obtained from asparagus in order to exert the effects of the present invention. When GABA exceeds 40% by mass, gastrointestinal disorder improving / preventing action is required. This is not preferable because the content of components other than GABA contained in the composition obtained from asparagus that brings about a synergistic additive effect is low.

  The food and drink having the gastrointestinal disorder improving / preventing action of the present invention needs to contain other components together with the composition obtained from asparagus. The other component is one or more substances selected from the group consisting of methylmethionine and / or derivatives thereof, niacin and / or derivatives thereof, vitamin B2, aloe, mucin and lactic acid bacteria.

  Methyl methionine and / or its derivatives may be methyl methionine, methyl methionine sulfonium chloride, or the like, or cabbage extract containing a high amount of methyl methionine, or cabbin series sold by Kowa Co., Ltd. may be used. Of these, methylmethionine sulfonium chloride is particularly preferred. These methylmethionine and / or derivatives thereof may be used by dissolving in the composition obtained from asparagus if it is liquid, and if the composition obtained from asparagus is powder, it is mixed therewith. Alternatively, it may be dissolved in water and mixed for use.

  Niacin and / or its derivative may be nicotinic acid, nicotinamide, or the like, and tryptophan, which is a precursor of niacin, may be added. If the composition obtained from asparagus is a liquid, it may be dissolved and used. If the composition obtained from asparagus is a powder, it may be mixed therewith or dissolved in water. You may mix and use.

  Vitamin B2 can be blended with riboflavin, but can also be blended as flavin nucleotides and flavin adenine nucleotides which are coenzyme types. If the composition obtained from asparagus is a liquid, it may be dissolved and used. If the composition obtained from asparagus is a powder, it may be mixed therewith or dissolved in water. You may mix and use.

  Aloe is a succulent plant belonging to the genus Aloe, and about 300 types are known, but any type may be used as long as it is edible. Of these, preferred are Kidachi aloe, Aloe vera, Aloe phoenix, Non-Night Castle, Aloe mubilis, and soap aloe, and particularly preferred are Kidachi aloe and Aloe vera. The form which mix | blends an aloe is not limited, A leaf may be used as it is, and you may use it after boiling and removing an accumulator. Moreover, an extract, dry powder, etc. can also be mix | blended. If the composition obtained from asparagus is a liquid, it may be dissolved or suspended in the composition. If the composition obtained from asparagus is a powder, it may be mixed as it is or water. They may be dissolved or suspended in and mixed for use.

  Mucin is a kind of glycoprotein, and is known to be mucus covering the inner tract of gastrointestinal tract such as trachea and gastrointestinal tract and gonad. In the present invention, mucin recovered from animals such as pigs and cows may be used, or mucin recovered from okra, natto, yam, etc. may be used. The mucin may be purified or contained as a mixture. The form which mix | blends a mucin is not limited, It can also mix | blend with forms, such as an extract and dry powder. If the composition obtained from asparagus is a liquid, it may be dissolved or suspended in the composition. If the composition obtained from asparagus is a powder, it may be mixed as it is or water. They may be dissolved or suspended in and mixed for use.

  Lactic acid bacteria can be used as long as they are safe for use in foods, and examples thereof include Lactobacillus, Lactococcus, Streptococcus, Enterococcus, and Bifidobacterium. Examples of such lactic acid bacteria include Lactobacillus brevis, Lactobacillus hirugardi, Lactobacillus plantarum, Lactobacillus helveticas, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus bulgaricus, Lactobacillus bulgaricus , Lactobacillus acidophilus, Streptococcus lactis, Streptococcus thermophilus, Enterococcus faecalis, Enterococcus casseri flavus, Lactococcus cremoris, Lactococcus lactis, Pediococcus damnosis, Bifidobacterium bifidum, Bifidobacterium adrecentis etc. are mentioned, among these Lactobacillus brevis, Lactobacillus hirugardi, Cactobacillus plantarum, Lactobacillus helveticus, Lactobacillus casei, Lactobacillus paracasei, Streptococcus thermophilus, Enterococcus faecalis, Lactococcus cremiris, Lactococcus lactis, Bifidobacterium bifidum are preferred, GABA Lactobacillus brevis, Lactobacillus hirugardi, Lactobacillus plantarum, Lactobacillus plantarum, Lactobacillus casei, Lactobacillus paracasei having the ability to produce These lactic acid bacteria may be used in the state of a culture solution or may be processed into a powder form such as lyophilized powder. Further, killed bacteria sterilized by heat sterilization or the like may be added. However, when the effect as probiotics is expected in the intestine, it is preferable to use live bacteria. The form of blending lactic acid bacteria is not limited. If the composition obtained from asparagus is liquid, it can be suspended and used. If the composition obtained from asparagus is powder, it is mixed as it is. Alternatively, it may be suspended in water and mixed.

  In addition, those containing two or more of the above-mentioned substances, for example, cabbage, asparagus, turmeric, kale, moroheiya, celery, okra, broccoli, capsicum, broad bean, peanut, soybean, natto, tempeh, corn, lotus root, taro, enoki mushroom, Maitake, matsutake, sea cucumber, dried shiitake, buckwheat, black rice, blueberry, sea bream, sardine, yellowtail, mackerel, flying fish, puffer fish, thailand, oysters, clams, shijimi, salmon, beef liver, pork liver, yogurt, milk, egg, cheese Etc. can also be used.

  In the food and drink of the present invention, the content of the composition obtained from asparagus is such that the amount of GABA contained therein is 5 mg to 2000 mg / day / person, preferably 10 mg to 1000 mg / day / person, more preferably 10 mg to What is necessary is just to adjust so that it may become 100 mg / day / person. If it is in this range, a sufficient gastrointestinal disorder improving action and / or gastrointestinal disorder preventing action can be obtained.

  GABA is an inhibitory neurotransmitter present in the brain and spinal cord of mammals, and it has been confirmed in many mammals that blood pressure is lowered by ingestion. This blood pressure lowering effect is effective only for those who have developed hypertension, and does not lower blood pressure for healthy individuals and does not cause hypotension. GABA is also contained in plants such as germinated brown rice and tea leaves, fermented foods such as kimchi and takuan, in addition to the brain and spinal cord of mammals. However, there are no reports of side effects due to GABA intake.

  On the other hand, one or more substances selected from the group consisting of methylmethionine and / or a derivative thereof, niacin and / or a derivative thereof, vitamin B2, aloe, mucin and lactic acid bacteria, which are other components, impair the effects of the present invention. Unless otherwise specified, it may be contained in any ratio, but it is 0.01 to 1000 times, preferably 0.05 to 100 times, and more preferably, by weight with respect to the composition obtained from asparagus. The amount is 0.1 to 20 times. When the amount is less than this range, the effect of addition may not be sufficiently exhibited, and when the amount is more than this range, the effect of the present invention may no longer be obtained.

  The gastrointestinal disorder in the present invention is not particularly limited and includes stomach pain, abdominal pain, heartburn, nausea, hyperacidity, burping, stomach sag, stomach discomfort, abdominal bloating, diarrhea, constipation, gastric ulcer, small intestine ulcer , Including duodenal ulcer, large intestine ulcer, stomach cancer, small intestine cancer, duodenal cancer, colon cancer and the like.

  The form of the food or drink having the gastrointestinal disorder improving / preventing action of the present invention is not particularly limited, and is suitable for edible use such as granules, granules, capsules, gels, pastes, milks, suspensions, liquids, beverages, etc. What is necessary is just to shape | mold into a form. In addition, sugars, sugar alcohols, salts, fats and oils, amino acids, organic acids, fruit juices, vegetable juices, fragrances, alcohols, glycerin, and the like are added to improve the quality of the taste as long as the effects of the present invention are not impaired. be able to.

  Moreover, it can also be set as the food / beverage products of this invention by adding a required amount of an active ingredient to normal food / beverage products. Preferable examples of such foods and beverages include, for example, processed noodles such as udon and pasta, processed meat products such as ham and sausage, processed fishery products such as kamaboko and chikuwa, processed milk products such as butter, powdered milk and fermented milk, jelly Examples include desserts such as ice cream, processed foods such as breads, confectionery, and seasonings, and beverages such as soft drinks, alcohols, fruit juice beverages, vegetable juice beverages, milk beverages, carbonated beverages, and coffee beverages. It is done.

  EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples.

In the present invention, the GABA and amino acid contents are values determined by the following method. That is, it measured on the following conditions by the high performance liquid chromatography method (HPLC method), and detected using the fluorescence detector.
HPLC: Shimadzu Corporation LC-9A
Column: Shim-pack ISC-07 / S1504
Mobile phase: 0.2 N sodium citrate buffer (pH 2.2)
Flow rate: 0.3 ml / min Temperature: 55 ° C
Reaction solution: ortho-phthalaldehyde Detection wavelength: excitation wavelength 348 nm, fluorescence wavelength 450 nm

Production Example 1
1 L of water was added to 1 kg of green asparagus stalks, crushed with a mixer, and heat-treated at 100 ° C. for 1 hour in an autoclave. The resulting treatment liquid is squeezed using a nonwoven fabric to collect the asparagus pressurizing liquid, and further, diatomaceous earth is used as a filter aid, and suction filtration is performed using a filter paper (ADVANTEC Toyo No. 5C). An extract was obtained. The obtained extract was concentrated under reduced pressure 10 times by a desktop evaporator (manufactured by EYELA). By this operation, 180 ml of GABA-containing composition was obtained. The GABA and amino acid contents were as shown in Table 1.

Production Example 2
1 L of water was added to 1 kg of green asparagus young stalks, crushed with a mixer, and squeezed using a nonwoven fabric to obtain an asparagus pressing solution. This was further subjected to suction filtration using filter paper (ADVANTEC Toyo No. 5C) using diatomaceous earth as a filter aid to obtain a light brown extract. 1% by mass of yeast extract (manufactured by Asahi Food and Healthcare) was added to this extract and sterilized at 121 ° C. for 15 minutes in an autoclave. After allowing to cool, 2% by mass of sodium glutamate was added and dissolved by stirring well. A total amount of lactic acid bacteria (FERM P-20710) pre-cultured for 1 day in 15 ml of MRS medium (Difco) was added thereto, and static culture was performed at 30 ° C. for 24 hours. After culturing, the cells were collected with a centrifuge, and the supernatant filtered through a 0.45 μm membrane filter was concentrated 10 times with a desktop evaporator to obtain 164 ml of brown GABA-enriched asparagus extract. . The GABA and amino acid contents were as shown in Table 1.

Example 1
In Production Example 2, lactic acid bacteria were not collected and lyophilized as they were. As a result, 57.0 g of a brownish powdery gastrointestinal disorder ameliorating agent having a GABA content of 35% by mass was obtained.

Example 2
2.5 g of methylmethioninesulfonium chloride (manufactured by Nacalai Tesque) was dissolved in 100 ml of the GABA-enriched asparagus extract obtained in Production Example 2 to prepare a gastrointestinal disorder improving agent containing methylmethionine derivative.

Example 3
In 100 ml of the GABA-enriched asparagus extract obtained in Production Example 2, 2.5 g of nicotinamide (manufactured by Nacalai Tesque) was dissolved to prepare a niacin-containing gastrointestinal disorder improving agent.

Example 4
2.5 g of vitamin B2 (manufactured by Nacalai Tesque) was dissolved in 100 ml of GABA-enriched asparagus extract obtained in Production Example 2 to prepare a vitamin B2-containing gastrointestinal disorder improving agent.

Example 5
The aloe juice is prepared by dissolving 2.5 g of a 10-fold concentrated filtrate obtained by crushing aloe mesophyll into 100 ml of GABA-enriched asparagus extract obtained in Production Example 2 using a mixer and filtering through diatomaceous earth. A gastrointestinal disorder improving agent was prepared.

Example 6
2.5 g of porcine-derived mucin (manufactured by Wako Pure Chemical Industries, Ltd.) was dissolved in 100 ml of GABA-enriched asparagus extract obtained in Production Example 2 to prepare a mucin-containing gastrointestinal disorder improving agent.

Comparative Example 1
FYP medium (D-fructose 1%, peptone 0.5%, yeast extract 1%, sodium acetate trihydrate 0.2%, twin 80 0.05%, magnesium sulfate heptahydrate 0.02%, sulfuric acid Manganese tetrahydrate 10ppm, iron sulfate heptahydrate 10ppm, sodium chloride 10ppm) 1.5L was autoclaved, and the whole amount of lactic acid bacteria (FERM P-20710) pre-cultured in 15ml MRS medium (manufactured by DIFCO) was added. The culture was stationary at 30 ° C. for 24 hours. The culture solution was lyophilized as it was to obtain 65.3 g of lactic acid bacteria powder.

Comparative Example 2
In Example 2, 100 ml of water was added instead of 100 ml of GABA-enriched asparagus extract to produce a methylmethionine derivative-containing composition in the same manner.

Example 7
To 100 ml of the gastrointestinal disorder improving agent obtained in Example 2, 10 g of glycodextrin dextrin was added and mixed, freeze-dried and pulverized to obtain 24.4 g of a light brown powder.

Test example 1
Six-week-old male male Donryu (Nippon Charles River) was purchased and divided into 11 groups of 5 animals, each of which was produced once or twice for 6 consecutive days in Production Examples 1 and 2, Examples 1 to 6, The compositions described in Comparative Examples 1 and 2 and physiological saline were orally administered in the amounts shown in Table 1. After fasting overnight, 50 μl of 20% acetic acid aqueous solution was orally administered to form an acetic acid ulcer, and each sample was orally administered again for 7 days from the next day. The stomach was excised from the rat the day after the final administration, and the area of the ulcer was measured. The results are shown in Table 2.

In the said test, in each group of Examples 1-6, the area of the ulcer part became a value lower than the group which gave only the physiological saline, and showed the ulcer suppression rate of 25%-52%. The ulcer inhibiting effect was obtained even in the case of containing only the asparagus extract of the present invention as in Production Examples 1 and 2, but one selected from methylmethionine, niacin, vitamin B2, aloe, mucin, and lactic acid bacteria. A greater effect was obtained when the asparagus extract was used in combination. This is considered that the synergistic effect of the conventionally known gastrointestinal disorder improving agent and the asparagus-derived gastrointestinal disorder improving agent was observed.

Claims (3)

Containing a composition obtained from asparagus and one or more substances selected from the group consisting of methylmethionine and / or its derivatives, niacin and / or its derivatives, vitamin B2, aloe, mucin and lactic acid bacteria A food / beverage product that has the characteristics of improving and preventing gastrointestinal disorders. The food / beverage product which has a gastrointestinal disorder improvement / prevention effect | action of Claim 1 whose composition obtained from asparagus is an extract from a young asparagus stem. The food / beverage product which has a gastrointestinal disorder improvement / prevention effect | action of Claim 1 or 2 whose composition obtained from asparagus is a composition which contains 1 mass%-40 mass% of (gamma) -aminobutyric acid.