JP2008063282A - Composition for oral cavity - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition for oral cavity, consisting of an aqueous solution of platinum nano colloid and without agglomerating the platinum nano colloid during its preservation. <P>SOLUTION: This composition for oral cavity contains the platinum nano colloid having 1 to 5 nm mean particle diameter, a hyaluronic acid salt and water. The composition can be used as a liquid dental paste material, mouth wash, tooth surface-applying agent, etc. These compositions can be blended with, for example a thickening agent, a surfactant, a sweetener, a preservative, various active ingredients, a coloring matter, an aromatizing agent or the like as necessary. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to an oral composition having antioxidant ability.

  The surface of the tooth has plaque mainly consisting of bacteria and polysaccharides produced by the bacteria, and the acid stored in the tooth decalcifies the enamel of the tooth to generate caries, and then a specific bacteria It is said that the toxin produced by this causes gingivitis, periodontitis, and alveolar pyorrhea.

  On the other hand, the living body has an immune mechanism that mobilizes factors that induce biological defense reactions such as polymorphonuclear leukocytes and macrophages that are one of blood cell components against infection and invasion of these bacteria to eliminate bacteria. It is known that when the immune mechanism works, active oxygen is released from immune cells as a biological defense reaction and kills bacteria and the like, while active oxygen also destroys surrounding tissue cells. High concentrations of active oxygen not only damage mucosal tissue but also pain, healing failure after surgery, periodontal tissue destruction due to periodontal disease, pain and delayed healing of denture pressure ulcers, and even cellular DNA Therefore, the possibility of a high risk factor for oral cancer has been pointed out.

  As a composition for removing active oxygen in the oral cavity, there is an oral composition containing a salt of hyaluronic acid and an antioxidant (see Patent Document 1), but ascorbic acid, tocophenol acetate, carotenoids, etc. Antioxidants such as vitamins, catechins, and polyphenols can only remove one or two or three of them among the 11 types of active oxygen that are supposed to exist in the body. For example, ascorbic acid removes only superoxide and changes itself to dehydroascorbic acid which does not have an antioxidant action. As described above, in the combination of the conventional antioxidant and the salt of hyaluronic acid, although the specific active oxygen can be removed, it changes to a substance having no antioxidant action after the removal, so that the effect is limited. there were.

On the other hand, it is conventionally known that platinum nanocolloids have the ability to remove active oxygen (see, for example, Patent Document 2). Since platinum nanocolloid removes active oxygen using the catalytic action of platinum, it has an advantage that it can remove active oxygen as many times as it exists. Platinum nanocolloid has the ability to remove various types of active oxygen such as superoxide anion, superoxide anion radical, hydrogen peroxide, hydroxy radical, singlet oxygen, lipid peroxide radical, alcohol peroxide radical, and nitric oxide. have. However, when a conventional platinum nanocolloid is used as an aqueous solution, there is a problem in that the platinum nanocolloid aggregates during the storage period in the composition and the effect of removing active oxygen is reduced.
Japanese Patent Laid-Open No. 10-182390 International Publication No. 2005/023467

  An object of the present invention is to provide an oral composition comprising a platinum nanocolloid aqueous solution and having a high storage stability in which the platinum nanocolloid does not aggregate during the storage period.

  As a result of intensive studies to solve the above problems, the present inventors have found that the above problems can be solved by adding a predetermined amount of hyaluronic acid salt to an oral composition which is an aqueous solution of platinum nanocolloid. Was completed.

  That is, the present invention is an oral composition containing platinum nanocolloid having an average particle diameter of 1 to 5 nm, a predetermined amount of a salt of hyaluronic acid, and water.

  The composition for oral cavity according to the present invention is an excellent composition for oral cavity with high storage stability in which platinum nanocolloids do not aggregate during the storage period.

  The platinum nanocolloid according to the present invention is conventionally known as a substance having a singlet oxygen removing ability and may be present as an aqueous solution. For example, what is described in International Publication No. 2005/023467 can be used. The platinum nanocolloid concentration in the composition is preferably 0.1 to 10 μmol / L. If it is less than 0.1 μmol / L, the effect of removing active oxygen is low, and if it exceeds 10 μmol / L, there is a problem that the production cost is too high.

  The hyaluronic acid salt used in the oral composition according to the present invention has an effect of preventing the platinum nanocolloid from aggregating in an aqueous solution during storage. As the salt, a monovalent metal salt such as sodium or potassium can be used, and can be appropriately selected from substances that are safe for living bodies. The blending amount of the hyaluronic acid salt is preferably 0.1 to 2% by weight of the total composition, and if it is less than 0.1% by weight, it is difficult to obtain the effect, and even if it exceeds 2% by weight, the effect is not improved.

  The composition for oral cavity according to the present invention can be applied to dentifrices, mouthwashes, tooth surface coating agents, artificial saliva and the like. These applied product groups can contain, for example, thickeners, surfactants, sweeteners, preservatives, various active ingredients, pigments, fragrances and the like as necessary.

  Examples of the thickener include glycerin, sorbit, propylene glycol, polyethylene glycol, sodium carboxymethyl cellulose, hydroxyethyl cellulose, carrageenan, sodium alginate, xanthan gum, carbopol, guar gum, montmorillonite, gelatin, and fumed silica. It is preferable that 0.05 to 15% by weight of the viscous agent is blended in the composition for oral cavity.

  As the surfactant, an anionic surfactant, a cationic surfactant, and a nonionic surfactant are blended. Specifically, sodium lauryl sulfate, sodium α-olefin sulfonate, N-acyl sarcosinate, N- Examples thereof include acyl glutamate, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, N-acyl taurate, sucrose fatty acid ester, alkylol amide, polyoxyethylene sorbitan monostearate, and pluronic. These surfactants are preferably blended in the oral composition in an amount of 0.1 to 5% by weight.

  As sweeteners, those conventionally used in general oral compositions such as xylitol, sodium saccharin, stevioside, paramethoxycinnamic aldehyde, neohesperidyl dihydrochalcone, perilartine, xylit, maltite, lactit can be used. The sweetener is preferably blended in the oral composition in an amount of 0.1 to 20% by weight.

  As preservatives, benzoic acid esters such as butyl paraoxybenzoate, methyl paraoxybenzoate, ethyl paraoxybenzoate, sodium benzoate, benzoic acid, potassium sorbate, sodium dehydroate, salicylic acid, etc. can be used, and oral compositions It is preferable that 0.01 to 5 weight% is mix | blended in.

  Various active ingredients that have been used in conventional oral compositions can be used without particular limitation. For example, fluoride such as sodium fluoride, potassium fluoride, ammonium fluoride, stannous fluoride, sodium monofluorophosphate, potassium monofluorophosphate as an active ingredient for preventing caries. Water-soluble phosphate compounds such as orthophosphate potassium and sodium salts, sodium pyrophosphate, sodium polyphosphate, zeolite, and dextranase as active ingredients for preventing calculus deposition. As an active ingredient for preventing periodontal disease and bad breath, allantochlorohydroxyaluminum, hinokitiol, lysozyme chloride, glycyrrhizic acid and its salts, sodium chloride, ε-aminocaproic acid, isopropylmethylphenol, chlorhexidine salts, cetylpyridinium chloride, azulenesulfonic acid Sodium, copper chlorophyllin sodium, benzethonium chloride, dl-α-tocophenol acetate, triclosan. Other active ingredients include calcium chloride, sodium chloride, potassium phosphate, strontium chloride, potassium nitrate, potassium oxalate, aluminum lactate, hydroxamic acid and its derivatives, polyvinyl pyrrolidone, copper compounds such as copper gluconate, mutanase, amylase, dihydro There are extracts such as cholesterol, epidihydrocholesterin, trichlorocarbanilide, berberine, α-bisabolol, soft sugar beet extract, buckwheat extract, clove, rosemary, ugone, safflower. Furthermore, examples of the fragrance include l-menthol, carvone, anethole, limonene terpenes or derivatives thereof, and examples of the colorant include blue No. 1, yellow No. 4, titanium dioxide and the like. It is preferable that 0.01 to 10% by weight of various active ingredients is blended in the oral composition according to the present invention.

<Example>
"Examples 1 and 2, Comparative Examples 1 and 2"
According to the blending amounts shown in Table 1, Examples 1 and 2 in which a hyaluronic acid salt was added to a platinum nanocolloid aqueous solution (platinum nanocolloid: Concentration 1 mmol / L, manufactured by Seatec Co.) and Comparative Example 1 in which no hyaluronic acid salt was added Each oral composition of Comparative Example 2 in which sodium citrate was added without adding the hyaluronic acid salt (oral cleaning solution for supplying fluorine) was prepared. The presence or absence of aggregation of platinum nanocolloids and the ability to remove active oxygen were evaluated by the following evaluation methods. The results are shown in Table 1.

<Table 1> (parts by weight)

"Examples 3 and 4, Comparative Examples 3 and 4"
Examples 3 and 4 in which a salt of hyaluronic acid was added to a platinum nanocolloid aqueous solution according to the blending amount shown in Table 2, and Comparative Example 3 in which a salt of hyaluronic acid was not added. Sodium citrate was added without adding a salt of hyaluronic acid. Each composition for oral cavity of Comparative Example 4 (oral cleaning solution) was prepared.

<Table 2> (parts by weight)

  Each oral composition (artificial saliva) was prepared according to the blending amount shown in Table 3. The presence or absence of aggregation of platinum nanocolloid was evaluated by the following evaluation method. The results are shown in Table 3.

<Table 3> (Parts by weight)

<Aggregation evaluation method>
The prepared oral composition was stored for 7 days under the condition of 60 ° C., and the liquid level was visually confirmed from above. The case where one or more black spots, which are agglomerates of minute platinum nanocolloids, were confirmed was evaluated as “present” and the case where none was confirmed as “absent”.

<Removability of active oxygen>
Among the active oxygens that are said to be present in the body, the ability to remove active oxygen was evaluated using hydrogen peroxide, which is relatively stable and present in the body, as an index. Each 100 μL of 500 mmol / L of hydrogen peroxide was mixed with 200 μL of the oral composition of each example and comparative example and allowed to stand at 37 ° C. for 24 hours to prepare a sample. 150 μL of each sample diluted 1000 times with distilled water, 50 μL of oxalic acid diester (TDPO) and pyrene mixed solution are mixed and stirred, and the amount of luminescence for 40 seconds after mixing is measured with a luminescence sensor PSN (ATTO) to calculate the integrated value. (A). In addition, as a control, 50 μL of a TDPO / pyrene mixed solution was mixed and stirred in 150 μL of a 1000-fold diluted sample of distilled water added instead of the oral composition of each Example and Comparative Example, and the amount of light emitted for 40 seconds after mixing. Using the result (b) of calculating the integrated value, the ability to remove active oxygen was evaluated according to the following calculation formula. Since TDPO and hydrogen peroxide react to form 1,2-dioxetanedione, which reacts with pyrene to emit light, the more the amount of oxygen peroxide remaining in the measurement target, the greater the amount of light emitted.

Removal capacity of active oxygen (%) = (1-a / b) × 100

Claims (1)

  An oral composition comprising platinum nanocolloid having an average particle diameter of 1 to 5 nm, a hyaluronic acid salt, and water.