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JP2008054540A - Mayonnaise-style seasoning that does not contain refined fat and egg yolk - Google Patents

Mayonnaise-style seasoning that does not contain refined fat and egg yolk Download PDF

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JP2008054540A
JP2008054540A JP2006233007A JP2006233007A JP2008054540A JP 2008054540 A JP2008054540 A JP 2008054540A JP 2006233007 A JP2006233007 A JP 2006233007A JP 2006233007 A JP2006233007 A JP 2006233007A JP 2008054540 A JP2008054540 A JP 2008054540A
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seasoning
mayonnaise
soymilk
fermented
viscosity
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JP4646072B2 (en
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Toshinori Igarashi
俊教 五十嵐
Yasutaka Hashioka
靖隆 橋岡
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Kikkoman Corp
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Abstract

【課題】特殊な水溶性ヘミセルロースを添加したり、また豆乳を予めプロテアーゼで加水分解したりすることなく、長期保存しても均質安定性の優れた、精製油脂及び卵黄を含まないマヨネーズ様調味料を提供する。
【解決手段】粘度が6.0Pa・s以上の豆乳発酵物を食塩、食酢などを含有する水性調味液と混和し、均質化して、精製油脂及び卵黄を含まないpH3.0〜4.7のマヨネーズ風調味料を得る。このマヨネーズ風調味料全体に対して20〜70%(w/w)の豆乳発酵物と水性調味液を配合する場合には、特に均質安定性に優れ食感が良好なマヨネーズ風調味料が得られる。
【選択図】なし
The present invention relates to a mayonnaise-like seasoning that does not contain refined oils and egg yolks and has excellent homogeneous stability even when stored for a long time without adding special water-soluble hemicellulose or hydrolyzing soy milk with protease in advance. I will provide a.
[Solution] A fermented soybean milk having a viscosity of 6.0 Pa · s or more is mixed with an aqueous seasoning liquid containing salt, vinegar and the like, homogenized, and has a pH of 3.0 to 4.7 free of purified fat and egg yolk. Get a mayonnaise-like seasoning. When blending 20-70% (w / w) soymilk fermented product and aqueous seasoning liquid with respect to the whole mayonnaise-like seasoning, a mayonnaise-like seasoning having particularly excellent homogeneity and good texture is obtained. It is done.
[Selection figure] None

Description

本発明は、発酵豆乳を利用したマヨネーズ風調味料の改良に関し、精製油脂及び卵黄を含まず、長期保存しても均質安定性に優れたマヨネーズ風調味料に関する。   The present invention relates to an improvement of a mayonnaise-like seasoning using fermented soymilk, and relates to a mayonnaise-like seasoning that does not contain refined fats and egg yolks and is excellent in homogeneous stability even after long-term storage.

近年、中高年齢層の増加に伴い、健康に対する意識が非常に高まりをみせている。特に油脂はカロリーが高いため肥満の原因になり、また卵黄はコレステロールを多量に含んでいるため動脈硬化を引き起こす一因となる等の理由から、食生活においてこれらの摂取を低減することが望ましいとされている。
一方、豆乳を乳酸発酵して得られる豆乳発酵物を利用してマヨネーズ風調味料を得ることが従来知られている。またそのような調味料が酸性下で凝集やざらつき、分離などを生じやすいという欠点を解消するために、特殊な水溶性ヘミセルロースを添加する方法(例えば、特許文献1参照)、あるいは豆乳を予めプロテアーゼで酵素分解し、次いで乳酸菌による乳酸発酵を行って得られた豆乳発酵物を利用する方法(例えば、特許文献2参照)が知られている。しかし、豆乳発酵物を利用したマヨネーズ風調味料であって、前記のような工程を必要としない、長期保存しても均質安定性に優れた精製油脂及び卵黄を含まないマヨネーズ風調味料は知られていない。
特開2001−29038号公報 特開昭61−63260号公報
In recent years, awareness of health has increased greatly with the increase of middle-aged and elderly. In particular, fats and oils cause obesity due to high calories, and egg yolks contain a large amount of cholesterol, which may cause arteriosclerosis. Has been.
On the other hand, it is conventionally known to obtain a mayonnaise-like seasoning using a fermented soymilk obtained by lactic fermentation of soymilk. In addition, in order to eliminate the disadvantage that such a seasoning tends to cause aggregation, roughness, separation, etc. under acidic conditions, a method of adding a special water-soluble hemicellulose (see, for example, Patent Document 1) or soy milk in advance with protease There is known a method (for example, see Patent Document 2) using a soymilk fermented product obtained by enzymatic degradation with lactic acid bacteria followed by lactic acid fermentation with lactic acid bacteria. However, it is a mayonnaise-style seasoning that uses fermented soymilk and does not require the above-mentioned process. It is not done.
JP 2001-29038 A JP 61-63260 A

本発明は、特殊な水溶性ヘミセルロースを添加したり、また豆乳をプロテアーゼで加水分解したりすることなく、長期保存しても均質安定性の優れた、精製油脂及び卵黄を含まないマヨネーズ風調味料を得ることを課題とする。なお、本発明でいう精製油脂とは、動物組織又は油糧種子(大豆、ナタネ、ヒマワリ、ゴマ、ピーナッツ等)から搾油又は抽出などにより分離した油脂を意味する。   The present invention is a mayonnaise-like seasoning that does not contain purified oil and egg yolk and has excellent homogeneity and stability even if stored for a long time without adding special water-soluble hemicellulose or hydrolyzing soymilk with protease. It is a problem to obtain. In addition, the refined fats and oils referred to in the present invention means fats and oils separated from animal tissues or oil seeds (soybean, rapeseed, sunflower, sesame, peanuts, etc.) by extraction or extraction.

本発明者らは、前記課題解決のために鋭意研究を重ねた結果、豆乳発酵物を利用してマヨネーズ風調味料を得るにあたり、粘度が6.0Pa・s以上の豆乳発酵物を食塩、食酢などを含有する水性調味液と均質に混和するときは、特殊な水溶性ヘミセルロースなどを添加したり、また予め豆乳をプロテアーゼで加水分解したりすることなく、長期保存しても均質安定性に優れた、精製油脂及び卵黄を含まないマヨネーズ風調味料が得られることを知り、これらの知見に基づいて本発明を完成した。   As a result of intensive studies for solving the above problems, the present inventors have obtained a soymilk fermented product having a viscosity of 6.0 Pa · s or more when using a fermented soymilk product with salt, vinegar Homogeneous stability with long-term storage without adding special water-soluble hemicellulose or pre-hydrolyzed soymilk with protease. In addition, it was found that a mayonnaise-like seasoning containing no refined fat and egg yolk was obtained, and the present invention was completed based on these findings.

すなわち本発明は以下に関する。
(1)粘度が6.0Pa・s以上の豆乳発酵物と水性調味液とを含有し、精製油脂及び卵黄を含まないマヨネーズ風調味料。
(2)粘度が6.0Pa・s以上の豆乳発酵物をマヨネーズ風調味料全体に対し20〜70%(w/w)含有する上記(1)に記載のマヨネーズ風調味料。
That is, the present invention relates to the following.
(1) A mayonnaise-style seasoning containing a fermented soymilk having a viscosity of 6.0 Pa · s or more and an aqueous seasoning liquid, and not containing refined fats and egg yolks.
(2) The mayonnaise-like seasoning according to (1) above, wherein the fermented soybean milk having a viscosity of 6.0 Pa · s or more is contained in an amount of 20 to 70% (w / w) of the whole mayonnaise-like seasoning.

本発明によれば、マヨネーズの原料でありながらその摂取量の低減化が望まれている精製油脂及び卵黄を添加することなく、マヨネーズ同様の均質安定性や食感を有するマヨネーズ風調味料を提供することが可能になり、このような調味料は、油脂やコレステロールの過剰摂取が引き起こす疾病の予防の一助となることが期待される。   According to the present invention, it is possible to provide a mayonnaise-like seasoning that has the same homogenous stability and texture as mayonnaise without adding refined fats and egg yolks that are raw materials of mayonnaise but are desired to reduce their intake. Such a seasoning is expected to help prevent diseases caused by excessive intake of fats and fats and cholesterol.

以下、本発明の実施の形態について詳細に説明する。   Hereinafter, embodiments of the present invention will be described in detail.

(豆乳)
本発明のマヨネーズ風調味料に使用する、粘度が6.0Pa・s以上の豆乳発酵物を得るために使用する豆乳としては任意の豆乳が使用できるが、3%(w/w)以上、特に4〜16%(w/w)の大豆固形分及びシュークロース、マルトース、スタキオースまたはラフィノースから選択される1種以上の糖を3%(w/w)以上、特に5〜15%(w/w)含有する豆乳が好ましい。
(Soy milk)
As the soymilk used for obtaining the soymilk fermented product having a viscosity of 6.0 Pa · s or more, which is used in the mayonnaise-like seasoning of the present invention, any soymilk can be used, but 3% (w / w) or more, particularly 4-16% (w / w) of soy solids and one or more sugars selected from sucrose, maltose, stachyose or raffinose at least 3% (w / w), in particular 5-15% (w / w) ) The soymilk contained is preferred.

(乳酸菌)
本発明のマヨネーズ風調味料に使用する、粘度が6.0Pa・s以上の豆乳発酵物は、例えば豆乳に乳酸菌を添加して乳酸発酵することにより得ることができる。使用する乳酸菌としては、発酵によって、多糖等の粘性に寄与し得る物質を多く生産できる菌種が好ましい。そのような乳酸菌としては、例えば、Leuconostoc mesenteroides、Leuconostoc paramesenteroides、Leuconostoc pseudomesenteroides、Leuconostoc citreum、Leuconostoc dextranicum等が挙げられる。より具体的には、例えば、Leuconostoc pseudomesenteroides(ATCC12291)、Leuconostoc citreum(NRIC 1579)、Leuconostoc paramesenteroides(NISL 7218)が挙げられる。なお、上記菌種名に関し、「NRIC」とは東京農業大学応用生物科学部菌株保存室が保存する菌株を意味し、「NISL」とは、財団法人野田産業科学研究所が保存する菌株を意味する。
これらの乳酸菌は、豆乳の発酵工程において多糖を生産し、粘度6.0Pa・s以上の滑らかなクリーム状の物性を有する豆乳発酵物を安定的に製造することができるので好ましい。
(Lactic acid bacteria)
The soymilk fermented product having a viscosity of 6.0 Pa · s or more used in the mayonnaise-like seasoning of the present invention can be obtained, for example, by adding lactic acid bacteria to soymilk and performing lactic acid fermentation. As a lactic acid bacterium to be used, a microbial species capable of producing a large amount of substances that can contribute to viscosity such as polysaccharides by fermentation is preferable. Examples of such lactic acid bacteria include Leuconostoc mesenteroides, Leuconostoc paramesenteroides, Leuconostoc pseudomesenteroides, Leuconostoc citreum, Leuconostoc dextranicum, and the like. More specifically, examples include Leuconostoc pseudomesenteroides (ATCC12291), Leuconostoc citreum (NRIC 1579), and Leuconostoc paramesenteroides (NISL 7218). In addition, regarding the above bacterial species names, “NRIC” means a strain preserved by the Tokyo University of Agriculture, Department of Applied Biological Sciences, and the “NISL” means a strain preserved by the Noda Institute of Industrial Science. To do.
These lactic acid bacteria are preferable because they produce polysaccharides in the soymilk fermentation process and can stably produce fermented soymilk having a smooth creamy physical property with a viscosity of 6.0 Pa · s or more.

(乳酸発酵)
豆乳の発酵には公知の方法を用いればよく、例えば、豆乳に糖類を添加し、そこに前述の乳酸菌を加えて発酵させ、豆乳の粘性を高めるために好適な培養時間と培養条件下、例えば、30〜35℃で4時間以上の発酵を行わせ、適宜発酵物の粘度を測定しながら発酵させる方法が挙げられる。このようにして豆乳の粘度が6.0Pa・s以上、特に6.0〜20Pa・sである豆乳発酵物が得られる。
(Lactic acid fermentation)
A well-known method may be used for fermentation of soy milk.For example, saccharides are added to soy milk, and the above-mentioned lactic acid bacteria are added thereto for fermentation, and culture time and conditions suitable for increasing the viscosity of soy milk, for example, The method of making it ferment for 4 hours or more at 30-35 degreeC, and making it ferment while measuring the viscosity of a fermented material suitably is mentioned. In this way, a soymilk fermented product having a soymilk viscosity of 6.0 Pa · s or higher, particularly 6.0 to 20 Pa · s is obtained.

本発明のマヨネーズ風調味料において、粘度が6.0Pa・s以上である豆乳発酵物を使用することは重要である。すなわち、粘度が6.0Pa・s未満の豆乳発酵物を使用するときは、該豆乳発酵物と水性調味液とを混合した場合に、pH4.7以下の低pH条件下においては組成物(調味液)が凝集、分離を起こし、または起こし易くなる問題点を有するので好ましくない。これに対し粘度が6.0Pa・s以上である豆乳発酵物を使用するときは、特殊な水溶性ヘミセルロースなどを添加したり、また豆乳をプロテアーゼで加水分解したりすることなく、pH4.7以下の低pH条件下となる場合でも組成物が凝集、分離しなくなり、均質安定性に優れたマヨネーズ風調味料が得られる。
なお、粘度の測定は公知の方法、例えば、B型粘度計を用いて測定することができる。
In the mayonnaise-like seasoning of the present invention, it is important to use a fermented soymilk having a viscosity of 6.0 Pa · s or more. That is, when a soymilk fermented product having a viscosity of less than 6.0 Pa · s is used, when the soymilk fermented product and an aqueous seasoning liquid are mixed, the composition (seasoning) is performed under a low pH condition of pH 4.7 or less. (Liquid) is not preferred because it has the problem of causing aggregation or separation, or causing it to easily occur. On the other hand, when using a fermented soymilk having a viscosity of 6.0 Pa · s or more, pH 4.7 or less without adding special water-soluble hemicellulose or hydrolyzing soymilk with protease. Even under low pH conditions, the composition does not agglomerate and separate, and a mayonnaise-like seasoning excellent in homogeneous stability can be obtained.
The viscosity can be measured using a known method, for example, a B-type viscometer.

前記の豆乳発酵物は、例えば、調味料として摂食するのに好ましいクリーミーな(滑らかな)食感及びさわやかな酸味と豆乳由来のコクのある旨味を有するので、本発明のマヨネーズ風調味料の原料として好ましいものである。このような前記豆乳発酵物の特性が本発明のマヨネーズ風調味料における濃厚な味とクリーミーな(滑らかな)食感、及び長期保存に耐え得る高い均質安定性に寄与している。本発明におけるマヨネーズ風調味料とは、マヨネーズに類似の食感・風味・物性を有する調味料、マヨネーズを主原料とする調味料に類似の食感・風味・物性を有する各種調味料をいう。   The soymilk fermented product has, for example, a creamy (smooth) texture preferable for eating as a seasoning and a refreshing sourness and a rich umami derived from soymilk. It is preferable as a raw material. Such characteristics of the fermented soymilk contribute to the rich taste and creamy (smooth) texture of the mayonnaise-like seasoning of the present invention, and high homogeneity stability that can withstand long-term storage. The mayonnaise-like seasonings in the present invention refer to seasonings having a texture, flavor and physical properties similar to mayonnaise, and various seasonings having a texture, flavor and physical properties similar to seasonings mainly made from mayonnaise.

(水性調味液)
本発明で使用する水性調味液とは、任意の水性の調味液を意味し、例えば、米酢、穀物酢、果実酢、ワインビネガー等の醸造酢;濃口醤油、淡口醤油、白醤油等の醤油類;味醂、みりん風調味料等の甘味調味料;ワインや日本酒等の酒類;酢酸、乳酸、クエン酸、リンゴ酸、酒石酸等の有機酸溶液;食塩;グルタミン酸ナトリウム、イノシン酸ナトリウム、コハク酸ナトリウム等の化学調味料溶液;砂糖、水飴、蜂蜜等の甘味料溶液;清澄なだし汁や動物又は植物蛋白質加水分解物等のアミノ酸調味料;発酵調味料、酵母エキス、魚介類エキス、野菜抽出物、海草エキス等のエキス類等の1種又は2種以上を含有する水性調味液が挙げられる。
(Aqueous seasoning liquid)
The aqueous seasoning liquid used in the present invention means any aqueous seasoning liquid, for example, brewed vinegar such as rice vinegar, grain vinegar, fruit vinegar, wine vinegar; soy sauce such as dark soy sauce, light soy sauce, white soy sauce, etc. Sweet taste seasonings such as miso and mirin-style seasonings; Liquors such as wine and sake; Organic acid solutions such as acetic acid, lactic acid, citric acid, malic acid and tartaric acid; Salt; Sodium glutamate, sodium inosinate, sodium succinate Chemical seasoning solutions such as sugar; sweetener solutions such as sugar, syrup and honey; amino acid seasonings such as clear broth and animal or plant protein hydrolysates; fermented seasonings, yeast extracts, seafood extracts, vegetable extracts, An aqueous seasoning liquid containing one or more kinds of extracts such as seaweed extract can be mentioned.

(その他の原材料)
その他の原材料としては、一般的な乳化調味料、例えば、マヨネーズや各種乳化液状ドレッシング類に使用されている、例えば、食塩、糖類、味噌、辛子、胡椒、胡麻ペーストやにんにくペースト、ハーブ粉末、ナツメグ、セージ、タイム、シナモン、これらの抽出物等の香辛料、核酸系調味料、果汁、野菜や果実、種実類等、例えばトマト、にんじん、タマネギ、にんにく、ゴマ、リンゴ、ゆず、オレンジ、モモ、イチゴ、パイナップル、ミカン、ブドウ等の加工処理物(ピューレ、ペースト、練状物、細断粒状物、磨砕物、粉末等)が挙げられる。なお必要により、加工澱粉や寒天、キサンタンガム、コーンスターチ、ペクチン等の各種安定剤や増粘剤を適量添加することができる。
(Other raw materials)
Other raw materials include general emulsified seasonings such as mayonnaise and various emulsified liquid dressings, such as salt, sugar, miso, hot pepper, pepper, sesame paste and garlic paste, herb powder, nutmeg Sage, thyme, cinnamon, spices such as these extracts, nucleic acid seasonings, fruit juice, vegetables and fruits, seeds, etc., such as tomatoes, carrots, onions, garlic, sesame, apples, citrons, oranges, peaches, strawberries , Processed products such as pineapples, mandarin oranges and grapes (purees, pastes, pastes, shredded granules, ground products, powders, etc.). If necessary, an appropriate amount of various stabilizers and thickeners such as processed starch, agar, xanthan gum, corn starch, and pectin can be added.

(本発明のマヨネーズ風調味料の調製)
前記豆乳発酵物、前記水性調味液、及び必要に応じその他の原材料を混合して均質化し、有機酸又は食酢を用いてpHを3.0〜4.7に調整して本発明のマヨネーズ風調味料を調製する。本発明のマヨネーズ風調味料においてpHを4.7以下となるように調整することは重要であり、pHが4.7を超える場合、得られるマヨネーズ風調味料は酸味が不足し、嗜好性に劣るものとなる。
(Preparation of mayonnaise-like seasoning of the present invention)
The fermented soymilk, the aqueous seasoning liquid, and other ingredients as necessary are mixed and homogenized, and the pH is adjusted to 3.0 to 4.7 using an organic acid or vinegar, and the mayonnaise-like seasoning of the present invention is used. Prepare the ingredients. In the mayonnaise-like seasoning of the present invention, it is important to adjust the pH to be 4.7 or less, and when the pH exceeds 4.7, the resulting mayonnaise-like seasoning lacks sourness and is palatable. It will be inferior.

なお、このようなマヨネーズ風調味料の原料として、従来はサラダオイル、コーンオイル、サフラワーオイル等の各種精製油脂の1種又は2種以上を、例えば1〜70%使用するが、本発明のマヨネーズ風調味料はそのような精製油脂を添加しないことを特徴とする。すなわち本発明は、豆乳発酵物及び/またはその他の原料に由来するわずかな油脂分のみを利用して、良好な食感および風味を有する均質安定性に優れたマヨネーズ風調味料の製造を可能にし、精製油脂の添加による高カロリー化、高コレステロール化を抑制できる精製油脂無添加のマヨネーズ風調味料の提供を可能にする。   In addition, as a raw material of such a mayonnaise-like seasoning, 1 type or 2 types or more of various refined fats and oils, such as salad oil, corn oil, safflower oil, etc. are used conventionally, for example, 1-70%. The mayonnaise-like seasoning is characterized by not adding such refined fats and oils. That is, the present invention makes it possible to produce a mayonnaise-like seasoning that has a good texture and flavor and is excellent in homogeneous stability by using only a small amount of fat and oil derived from fermented soybean milk and / or other raw materials. The addition of refined fats and oils makes it possible to provide a mayonnaise-like seasoning free of refined fats and oils that can suppress the increase in calories and cholesterol.

(マヨネーズ風調味料の含有量の調整)
本発明のマヨネーズ風調味料においては、粘度が6.0Pa・s以上である豆乳発酵物を該マヨネーズ風調味料全量に対して5〜75%(w/w)の範囲で使用可能である。豆乳発酵物の使用量が非常に少ない場合、例えば、5%未満の場合は、精製油脂及び卵黄を使用せずにマヨネーズ同様の均質安定性を確保することが困難となる傾向がある。また、反対に豆乳発酵物の使用量が75%を越える場合には、水性調味液に対する豆乳の風味が濃厚となり、マヨネーズ風調味料としては風味がややかけ離れたものとなる。これに対し、豆乳発酵物の使用量が5〜75%(w/w)の範囲にあるときには、マヨネーズ風調味料として良好な風味を有しつつ、精製油脂及び卵黄を使用せずにマヨネーズ同様の均質安定性を確保することが可能である。より好ましくは豆乳発酵物の使用量は20〜70%(w/w)である。その場合には、pH4.7以下での均質安定性をより良好なものとすることができ、低pH条件下でも凝集、分離が起こりにくく、しかもその他の原材料との配合バランスがとれ良好な風味を有するマヨネーズ風調味料を得ることが可能となる。さらに、豆乳発酵物の使用量を40〜70%(w/w)とする場合においては、本発明のマヨネーズ風調味料は特に良好な均質安定性を有し、例えば澱粉等のいわゆる糊料の添加を低減あるいは省略してもマヨネーズ同様の粘度を有する。
(Adjustment of mayonnaise-style seasoning content)
In the mayonnaise-like seasoning of the present invention, fermented soymilk having a viscosity of 6.0 Pa · s or more can be used in a range of 5 to 75% (w / w) with respect to the total amount of the mayonnaise-like seasoning. When the amount of fermented soymilk used is very small, for example, less than 5%, it tends to be difficult to ensure homogeneous stability similar to mayonnaise without using refined fats and egg yolks. On the other hand, when the amount of fermented soymilk used exceeds 75%, the flavor of soymilk with respect to the aqueous seasoning liquid becomes thick, and the flavor of the mayonnaise-like seasoning is slightly different. On the other hand, when the amount of fermented soymilk used is in the range of 5 to 75% (w / w), it has a good flavor as a mayonnaise-like seasoning and is similar to mayonnaise without using refined oil and egg yolk. It is possible to ensure the homogeneous stability. More preferably, the amount of fermented soymilk used is 20 to 70% (w / w). In that case, the homogeneity stability at pH 4.7 or less can be improved, aggregation and separation are less likely to occur even under low pH conditions, and the blending balance with other raw materials is balanced, and the flavor is good. It is possible to obtain a mayonnaise-like seasoning having Furthermore, in the case where the amount of fermented soymilk used is 40 to 70% (w / w), the mayonnaise-like seasoning of the present invention has particularly good homogeneous stability, for example, a so-called paste such as starch. Even if the addition is reduced or omitted, it has the same viscosity as mayonnaise.

本発明のマヨネーズ風調味料全量に対する各素材の好適な配合比(%)を以下に示す。
豆乳発酵物 :20〜70%(w/w)
水飴 :15〜35%(w/w)
食酢(酢酸5%(w/w)) :5〜10%(w/w)
食塩 :2〜5%(w/w)
澱粉 :0〜5%(w/w)
香辛料 :0.1〜5%(w/w)
精製油脂 :含まず
卵黄 :含まず
pH :4.7〜3.0
The suitable compounding ratio (%) of each raw material with respect to the total amount of the mayonnaise-like seasoning of the present invention is shown below.
Fermented soybean milk: 20-70% (w / w)
Minamata: 15-35% (w / w)
Vinegar (acetic acid 5% (w / w)): 5-10% (w / w)
Salt: 2-5% (w / w)
Starch: 0-5% (w / w)
Spice: 0.1-5% (w / w)
Purified oil and fat: not included Egg yolk: not included pH: 4.7-3.0

以下、実験例及び実施例により、本発明をさらに具体的に説明する。但し、本発明の技術的範囲は、それらの例により何ら限定されるものではない。   Hereinafter, the present invention will be described more specifically with reference to experimental examples and examples. However, the technical scope of the present invention is not limited by these examples.

実験例1
1.粘度6.15Pa・sの豆乳発酵物の調製
1)豆乳の調製
フィルターろ過済みの20%シュークロース溶液を等量加えることで、終濃度において10%シュークロースを含有する大豆固形分7%の豆乳(以下、「発酵前の豆乳」と称する)を調製した。調製した上記豆乳を滅菌済みのカップ等の容器に充填した。
2)乳酸菌の調製
乳酸菌Leuconostoc paramesenteroides(NISL 7218)を乳酸菌用調製培地(1.0%大豆ペプトン、0.5%酵母エキス、1.0%グルコース、0.5%酢酸ナトリウム)にて30℃、20時間培養した。培養液10mlを遠心分離(3,000g×5min)して菌体を回収した。
3)発酵
上記で回収した菌体を、調製した豆乳に全量添加し、30℃、24時間恒温状態にて発酵を行い、本発明のマヨネーズ風調味料の製造に用いる豆乳発酵物を得た。
2.粘度0.4Pa・sの豆乳発酵物(比較例)の調製
豆乳中で多糖生成が低い菌種である、Leuconostoc mesenteroides(NISL 7201)を用いて、上記と同様に糖を含有する豆乳にて発酵を行い、比較例の豆乳発酵物を得た。
3.本発明のマヨネーズ風調味料に用いる豆乳発酵物及び比較例のマヨネーズ風調味料に用いる豆乳発酵物の評価
上記により得られた豆乳発酵物の粘度を、B型粘度計を用いて測定した(測定条件:No.3ローター、25℃、12−60回転)。表1に示すように、Leuconostoc paramesenteroides(NISL 7218)を用いて発酵させた場合は、6.15Pa・sという高い粘度を有する豆乳発酵物が得られた。一方、Leuconostoc mesenteroides(NISL 7201)を用いて発酵させた場合には、0.4Pa・sの粘度を有する豆乳発酵物が得られたが、本発明に用いる豆乳発酵物のように高粘度の豆乳発酵物を得ることができなかった。
Experimental example 1
1. Preparation of soymilk fermented product with a viscosity of 6.15 Pa · s 1) Preparation of soymilk Soymilk containing 7% soybean solids containing 10% sucrose at a final concentration by adding an equal amount of 20% sucrose solution that has been filtered and filtered (Hereinafter referred to as “soy milk before fermentation”). The prepared soymilk was filled in a container such as a sterilized cup.
2) Preparation of lactic acid bacterium Lactic acid bacterium Leuconostoc paramesenteroides (NISL 7218) was prepared at 30 ° C. in a lactic acid bacterium preparation medium (1.0% soybean peptone, 0.5% yeast extract, 1.0% glucose, 0.5% sodium acetate). Cultured for 20 hours. 10 ml of the culture solution was centrifuged (3,000 g × 5 min) to recover the cells.
3) Fermentation The whole amount of the microbial cells collected above was added to the prepared soymilk and fermented in a constant temperature state at 30 ° C. for 24 hours to obtain a fermented soymilk product used in the production of the mayonnaise-like seasoning of the present invention.
2. Preparation of fermented soybean milk (comparative example) with a viscosity of 0.4 Pa · s Fermented with soy milk containing sugar in the same manner as above using Leuconostoc mesenteroides (NISL 7201), a bacterial species with low polysaccharide production in soy milk The soymilk fermented product of the comparative example was obtained.
3. Evaluation of soymilk fermented product used in the mayonnaise-like seasoning of the present invention and soymilk fermented product used in the mayonnaise-like seasoning of the comparative example The viscosity of the soymilk fermented product obtained as described above was measured using a B-type viscometer (measurement) Conditions: No. 3 rotor, 25 ° C., 12-60 rotations). As shown in Table 1, when fermented using Leuconostoc paramesenteroides (NISL 7218), fermented soymilk having a high viscosity of 6.15 Pa · s was obtained. On the other hand, when fermented using Leuconostoc mesenteroides (NISL 7201), a soymilk fermented product having a viscosity of 0.4 Pa · s was obtained, but a highly viscous soymilk like the fermented soymilk used in the present invention was obtained. A fermented product could not be obtained.

Figure 2008054540
Figure 2008054540

(本発明1)
実験例1で得られた粘度6.15Pa・sの豆乳発酵物を用いて、以下に示す配合割合で各原材料を混合し、マヨネーズ風調味料(本発明1)を得た。
<配合割合>
成分 配合1 配合2 配合3 配合4 配合5
豆乳発酵物 40 70 100 120 140
食酢 20 20 20 20 20
水 140 110 80 60 40
注1:食酢(水性調味液)は、5%(w/w)酢酸濃度のリンゴ酢を使用。
注2:配合量はgで記載。
(Invention 1)
Using the fermented soybean milk having a viscosity of 6.15 Pa · s obtained in Experimental Example 1, raw materials were mixed at the following blending ratio to obtain a mayonnaise-like seasoning (present invention 1).
<Combination ratio>
Ingredients Formula 1 Formula 2 Formula 3 Formula 4 Formula 5
Fermented soymilk 40 70 100 120 140
Vinegar 20 20 20 20 20
Water 140 110 80 60 40
Note 1: Apple vinegar with 5% (w / w) acetic acid concentration is used as vinegar (aqueous seasoning liquid).
Note 2: The compounding amount is described in g.

(比較例1)
また、比較のため上記実施例1の方法において、「粘度6.15Pa・sの豆乳発酵物」を用いる代わりに、実験例1の比較例で得られた「粘度0.4Pa・sの豆乳発酵物」を用いる以外は全く同様にして、マヨネーズ風調味料(比較例1)を得た。
(Comparative Example 1)
For comparison, in the method of Example 1 above, instead of using “fermented soymilk with a viscosity of 6.15 Pa · s”, the “fermented soymilk with a viscosity of 0.4 Pa · s” obtained in the comparative example of Experimental Example 1 was used. A mayonnaise-like seasoning (Comparative Example 1) was obtained in exactly the same manner except that the product was used.

(対照1)
また、対照として、上記実施例1の方法において「粘度6.15Pa・sの豆乳発酵物」を用いる代わりに、実験例1で用いた「発酵前の豆乳」を用いる以外は全く同様にして、マヨネーズ風調味料(対照1)を得た。
(Control 1)
In addition, as a control, instead of using the “fermented soymilk with a viscosity of 6.15 Pa · s” in the method of Example 1, the same procedure as in Example 1 was used except that the “pre-fermented soymilk” was used. A mayonnaise-like seasoning (Control 1) was obtained.

上記の本発明1、比較例1及び対照1のマヨネーズ風調味料を各10ml分取し、15ml容遠心分離用試験管に入れ、5℃、20分間、1,000rpmにて遠心後、分離状態を目視観察すると共に、分離された透明な上層の液量を測定した。
また、上層以外の不透明部分(沈殿、ふわふわとした中間層、および遠心分離前の乳化状態と同様の液状部分を含む)を下層として、その量を測定した。
マヨネーズ風調味料全体に対する上層の割合(x%)、及び下層の割合((100−x)%)を算出し、分離状態(乳化安定性)の尺度とした。
以上の遠心分離及び静置保存による乳化安定性評価結果を表2〜表6に示す。
10 ml each of the above-mentioned present invention 1, comparative example 1 and control 1 mayonnaise-like seasonings were collected, put into a 15 ml test tube for centrifugation, centrifuged at 1,000 rpm at 5 ° C. for 20 minutes, and then separated. Was visually observed, and the liquid amount of the separated transparent upper layer was measured.
Further, the amount of the non-transparent portion other than the upper layer (including a precipitate, a fluffy intermediate layer, and a liquid portion similar to the emulsified state before centrifugation) was measured as the lower layer.
The ratio of the upper layer (x%) and the ratio of the lower layer ((100-x)%) to the whole mayonnaise-like seasoning were calculated and used as a measure of the separation state (emulsification stability).
Tables 2 to 6 show the results of evaluation of emulsion stability by centrifugation and stationary storage.

Figure 2008054540
Figure 2008054540

Figure 2008054540
Figure 2008054540

Figure 2008054540
Figure 2008054540

Figure 2008054540
Figure 2008054540

Figure 2008054540
Figure 2008054540

表2〜表5の結果から、粘度が0.4Pa・sの豆乳発酵物を用いたマヨネーズ風調味料(比較例1の区分)及び乳酸発酵処理しない無処理の「豆乳」を用いたマヨネーズ風調味料(対照1の区分)においては固液分離が認められる欠点を有することがわかる。これに対し、粘度が6.0Pa・s以上の豆乳発酵物を用いたマヨネーズ風調味料は、配合1〜配合5いずれにおいても固液分離は全く起こらず、いずれも均質安定性が非常に高いことがわかる。
さらに、この遠心分離後の試験管を垂直に立てて室温にて7日間静置保存後に、分離状態を確認したところ、比較例1ではさらに経時的な分離が進行していたのに比べ、本発明では分離の進行がほとんど確認されず、良好な均質安定性が維持されていた。なお、対照においては最初の遠心分離段階で十分に沈殿を形成していたため、その後の保存による分離の進行はほとんど見られなかった。
以上の結果から、6.0Pa・s以上である豆乳発酵物を水性調味液と混合してマヨネーズ風調味料を調製する時には、pH4.7以下の低pH条件下となる場合でも凝集、分離が起こらず均質安定性に優れたマヨネーズ風調味料が得られることがわかる。
From the results in Tables 2 to 5, the mayonnaise-style seasoning (category of Comparative Example 1) using a soymilk fermented product having a viscosity of 0.4 Pa · s and the untreated “soymilk” not treated with lactic acid fermentation It can be seen that the seasonings (control 1 category) have the disadvantage that solid-liquid separation is observed. On the other hand, the mayonnaise-style seasoning using a soymilk fermented product having a viscosity of 6.0 Pa · s or higher does not cause any solid-liquid separation in any of Formulations 1 to 5, and the homogeneity stability is very high in all cases. I understand that.
Furthermore, when the separated test state was confirmed after standing this test tube vertically after standing at room temperature for 7 days, the separation state was confirmed. In the invention, almost no progress of separation was confirmed, and good homogeneous stability was maintained. In the control, since a precipitate was sufficiently formed at the first centrifugation stage, the progress of separation due to subsequent storage was hardly observed.
From the above results, when preparing a mayonnaise-like seasoning by mixing a soymilk fermented product of 6.0 Pa · s or more with an aqueous seasoning liquid, aggregation and separation may occur even under low pH conditions of pH 4.7 or lower. It can be seen that a mayonnaise-like seasoning that does not occur and has excellent homogeneous stability can be obtained.

(本発明2)
配合割合
粘度6.15Pa・sの豆乳発酵物:300g
水飴 :230g
食塩 :34g
リンゴ酢(酢酸5%(w/w)) :80ml
加工澱粉 :28g
小麦発酵調味料(粉末) :10g
香辛料(マスタード) :2g
黄色素(着色料) :0.5g
水 :合計容量が1000mlとなるように添加
合計値 :1000ml
上記配合割合にて各原料を混和して均質化させ、pH4.3のマヨネーズ風調味料(本発明2)を製造した。
得られたマヨネーズ風調味料は良好な均質安定性を示し、食感は滑らかでマヨネーズの風味も良好であった。また、このマヨネーズ風調味料を200ml容ガラス製密封容器に入れ、室温にて4ヶ月間静置した時も分離は観察されず、均質性が維持されていた。
(Invention 2)
Blending ratio Soymilk fermented product with a viscosity of 6.15 Pa · s: 300 g
Minamata: 230g
Salt: 34g
Apple cider vinegar (acetic acid 5% (w / w)): 80ml
Processed starch: 28g
Fermented wheat seasoning (powder): 10g
Spice (Mustard): 2g
Yellow element (coloring agent): 0.5 g
Water: Added so that the total volume is 1000 ml. Total value: 1000 ml
Each raw material was mixed and homogenized at the above blending ratio to produce a mayonnaise-style seasoning (Invention 2) having a pH of 4.3.
The obtained mayonnaise-like seasoning showed good homogeneous stability, the texture was smooth and the mayonnaise flavor was also good. Further, even when this mayonnaise-like seasoning was put in a 200 ml glass sealed container and allowed to stand at room temperature for 4 months, no separation was observed and the homogeneity was maintained.

(比較例2)
比較のため、実施例2のマヨネーズ風調味料の調製において、「粘度6.15Pa・sの豆乳発酵物300g」を使用する代わりに、上記実験例1で得られた「粘度0.4Pa・sの豆乳発酵物300g」を用いる以外は、全く同様にして比較例2のマヨネーズ風調味料を得た。得られたマヨネーズ風調味料は、本発明2のものと比べて、均質でなく、舌触りがざらついた感じであり、マヨネーズ風調味料としての風味もやや劣るものであった。
(Comparative Example 2)
For comparison, in the preparation of the mayonnaise-style seasoning of Example 2, instead of using “300 g of fermented soymilk with a viscosity of 6.15 Pa · s”, the “viscosity of 0.4 Pa · s obtained in Experimental Example 1 above” was used. The mayonnaise-like seasoning of Comparative Example 2 was obtained in exactly the same manner except that 300 g of the soymilk fermented product was used. The obtained mayonnaise-like seasoning was not homogeneous and had a rough texture as compared with the present invention 2, and the flavor as a mayonnaise-like seasoning was slightly inferior.

(本発明3)
配合割合
粘度6.15Pa・sの豆乳発酵物:500g
水飴 :230g
食塩 :34g
リンゴ酢(酢酸5%(w/w)) :80ml
小麦発酵調味料(粉末) :10g
香辛料(マスタード) :2g
黄色素(着色料) :0.5g
水 :合計容量が1000mlとなるように添加
合計値 :1000ml
上記配合割合にて各原料を混和して均質化させ、pH4.3のマヨネーズ風調味料(本発明3)を製造した。
得られたマヨネーズ風調味料は良好な均質安定性を示し、食感は滑らかでマヨネーズの風味も良好であった。また、このマヨネーズ風調味料を200ml容ガラス製密封容器に入れ、室温にて4ヶ月間静置した時も分離は観察されず、均質性が維持されていた。
(Invention 3)
Blending ratio Soymilk fermented product with a viscosity of 6.15 Pa · s: 500 g
Minamata: 230g
Salt: 34g
Apple cider vinegar (acetic acid 5% (w / w)): 80ml
Fermented wheat seasoning (powder): 10g
Spice (Mustard): 2g
Yellow element (coloring agent): 0.5 g
Water: Added so that the total volume is 1000 ml. Total value: 1000 ml
Each raw material was mixed and homogenized at the above blending ratio to produce a mayonnaise-like seasoning (Invention 3) having a pH of 4.3.
The obtained mayonnaise-like seasoning showed good homogeneous stability, the texture was smooth and the mayonnaise flavor was also good. Further, even when this mayonnaise-like seasoning was put in a 200 ml glass sealed container and allowed to stand at room temperature for 4 months, no separation was observed and the homogeneity was maintained.

(比較例3)
比較のため、実施例3のマヨネーズ風調味料の調製において、「粘度6.15Pa・sの豆乳発酵物500g」を使用する代わりに、上記実験例1で得られた「粘度0.4Pa・sの豆乳発酵物500g」を用いる以外は、全く同様にして比較例2のマヨネーズ風調味料を得た。得られたマヨネーズ風調味料は、本発明3のものと比べて、均質でなく、舌触りがざらついた感じであり、マヨネーズ風調味料としての風味もやや劣るものであった。
(Comparative Example 3)
For comparison, in the preparation of the mayonnaise-like seasoning of Example 3, instead of using “500 g of soymilk fermented product with a viscosity of 6.15 Pa · s”, the “viscosity of 0.4 Pa · s obtained in Experimental Example 1 above” was used. The mayonnaise-like seasoning of Comparative Example 2 was obtained in the same manner except that 500 g of soymilk fermented product was used. The obtained mayonnaise-like seasoning was not homogeneous and rough to the touch as compared with the invention 3, and the flavor as a mayonnaise-like seasoning was slightly inferior.

Claims (2)

粘度が6.0Pa・s以上の豆乳発酵物と水性調味液とを含有し、精製油脂及び卵黄を含まないマヨネーズ風調味料。   A mayonnaise-like seasoning containing a fermented soymilk having a viscosity of 6.0 Pa · s or more and an aqueous seasoning liquid, and containing no refined fat or egg yolk. 粘度が6.0Pa・s以上の豆乳発酵物をマヨネーズ風調味料全体に対し20〜70%(w/w)含有する請求項1に記載のマヨネーズ風調味料。
The mayonnaise-like seasoning according to claim 1, comprising 20 to 70% (w / w) of a soymilk fermented product having a viscosity of 6.0 Pa · s or more with respect to the whole mayonnaise-like seasoning.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD73Z (en) * 2008-03-28 2010-04-30 Технический университет Молдовы Mayonnaise

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JPS56148258A (en) * 1980-04-18 1981-11-17 Kimie Miyazaki Preparation of pasty salad dressing
JPS5955166A (en) * 1982-09-21 1984-03-30 Kibun Kk Preparation of mayonnaise-like vegetable dressing
JPS6163260A (en) * 1984-09-04 1986-04-01 Marukin Shoyu Kk Preparation of emulsion having excellent emulsion stability
JP2001029038A (en) * 1999-07-21 2001-02-06 Fuji Oil Co Ltd Dressings and their production method
JP2003102424A (en) * 2001-09-28 2003-04-08 Nippon Maruten Shoyu Kk Soybean milk seasoning of oil-free type and method for producing the same
JP2007014303A (en) * 2005-07-11 2007-01-25 Kikkoman Corp Soymilk fermented food and method for producing the same
JP2007319129A (en) * 2006-06-05 2007-12-13 Kikkoman Corp Seasoned liquid containing fermented soymilk
JP2007319130A (en) * 2006-06-05 2007-12-13 Kikkoman Corp Seasoned liquid containing fermented soymilk
JP2007325544A (en) * 2006-06-08 2007-12-20 Kikkoman Corp Homogeneous composition containing fermented soymilk
JP4540071B2 (en) * 2006-08-04 2010-09-08 キッコーマン株式会社 Mayonnaise-style seasoning that does not contain refined fat and egg yolk

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Publication number Priority date Publication date Assignee Title
JPS56148258A (en) * 1980-04-18 1981-11-17 Kimie Miyazaki Preparation of pasty salad dressing
JPS5955166A (en) * 1982-09-21 1984-03-30 Kibun Kk Preparation of mayonnaise-like vegetable dressing
JPS6163260A (en) * 1984-09-04 1986-04-01 Marukin Shoyu Kk Preparation of emulsion having excellent emulsion stability
JP2001029038A (en) * 1999-07-21 2001-02-06 Fuji Oil Co Ltd Dressings and their production method
JP2003102424A (en) * 2001-09-28 2003-04-08 Nippon Maruten Shoyu Kk Soybean milk seasoning of oil-free type and method for producing the same
JP2007014303A (en) * 2005-07-11 2007-01-25 Kikkoman Corp Soymilk fermented food and method for producing the same
JP2007319129A (en) * 2006-06-05 2007-12-13 Kikkoman Corp Seasoned liquid containing fermented soymilk
JP2007319130A (en) * 2006-06-05 2007-12-13 Kikkoman Corp Seasoned liquid containing fermented soymilk
JP2007325544A (en) * 2006-06-08 2007-12-20 Kikkoman Corp Homogeneous composition containing fermented soymilk
JP4570097B2 (en) * 2006-06-08 2010-10-27 キッコーマン株式会社 Homogeneous composition containing fermented soymilk
JP4540071B2 (en) * 2006-08-04 2010-09-08 キッコーマン株式会社 Mayonnaise-style seasoning that does not contain refined fat and egg yolk

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD73Z (en) * 2008-03-28 2010-04-30 Технический университет Молдовы Mayonnaise

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