JP2007271361A - Measuring apparatus and sensor unit holding method - Google Patents

Abstract

A sensor unit is prevented from being displaced when a pipette is inserted and removed.
A mounting table 43 on which a sensor unit 11 is mounted is provided with a positioning member 77 having a reference surface 77a and a pressing member 81 having a pressing surface 81a. The reference surface 77a is inclined at an angle of 45 degrees toward the placement surface 43a, and comes into contact with the convex portion 23b of the prism 23. The pressing surface 81a is inclined at an angle symmetric to the reference surface 77a. The pressing member 81 is provided so as to be movable up and down in the Z-axis direction perpendicular to the placement surface 43a. When the pressing member 81 is lowered, the pressing surface 81a and the convex portion 23b of the prism 23 come into contact with each other and press it. As a result, the other protrusion 23b of the prism 23 is pressed against the reference surface 77a. As a result, the prism 23 is sandwiched between the pressing surface 81a and the reference surface 77a, is positioned in the Y-axis direction, and is pressed against the placement surface 43a by a force acting in the Z-axis direction.
[Selection] Figure 9

Description

  The present invention relates to a measuring apparatus that optically measures a reaction of a sample using a sensor unit having an optical block and a method for holding the sensor unit.

  2. Description of the Related Art Measuring apparatuses that optically measure the reaction of a sample are known in order to measure interactions between biochemical substances such as proteins and DNA, and to screen for drugs.

  As one of such measuring apparatuses, there is a measuring apparatus (hereinafter referred to as an SPR measuring apparatus) using a surface plasmon resonance phenomenon. The surface plasmon is generated by the collective vibration of free electrons in the metal, and is a density wave of free electrons traveling along the surface of the metal.

  For example, in an SPR measuring apparatus employing a Kretschmann arrangement known from Patent Document 1 or the like, a transparent dielectric block (hereinafter referred to as a prism) is used as an optical block, and the surface of a metal film formed on the prism is used. As the sensor surface, while reacting the sample on the sensor surface, the metal film is irradiated from the back surface side of the sensor surface through the prism so as to satisfy the total reflection condition, and the reflected light is measured.

  Of the light irradiated to the metal film so as to satisfy the total reflection condition, a small amount of light called an evanescent wave passes through the metal film without being reflected and oozes out to the sensor surface side. At this time, if the frequency of the evanescent wave coincides with the frequency of the surface plasmon, SPR is generated and the intensity of the reflected light is greatly attenuated. In addition, the incident angle (resonance angle) of light at which this attenuation occurs varies depending on the refractive index on the metal film. That is, the SPR measurement device measures the reaction state of the sample on the sensor surface by capturing the reflected light from the metal film and detecting the resonance angle.

  By the way, biological samples such as proteins and DNA are often handled as sample solutions dissolved in a solvent such as physiological saline, pure water, or various buffer solutions in order to prevent denaturation and inactivation due to drying. The SPR measurement device described in Patent Document 1 is for examining such interaction between biological samples, and a flow path for feeding a sample solution is provided on the sensor surface. The flow path and the prism are arranged on a measurement stage provided in the apparatus main body, and the above-described measurement can be performed by mounting a chip-type sensor unit having a metal film formed on a glass substrate on the measurement stage. Done.

  In Patent Document 1, the sample solution is directly fed into the flow path from a container for storing the sample solution via a pipe (tube) connected to a pump, a valve, or the like. There is a problem that so-called contamination is likely to occur, in which the sample is mixed into a sample solution to be injected later.

  In order to solve this problem, the present applicant uses a pipette consisting of a substantially conical cylindrical pipette tip with a small hole formed at the tip and a head portion that detachably holds the pipette tip, and attaches it to the container. We are investigating the development of an SPR measurement device that dispenses liquids such as stored sample solutions into the flow path. In this SPR measurement device, contamination occurring when liquid is fed into the flow path can be prevented by exchanging the pipette tip for each liquid to be dispensed.

Further, in this SPR measurement device, the flow path member in which the flow path is formed, the prism having the metal film formed on the upper surface, and the bottom surface of the flow path member and the upper surface of the prism are joined (the flow path and the metal A sensor unit including a holding member that holds the film in a state of facing the film is used. The flow path is a liquid feed pipe formed by penetrating the flow path member in a substantially U shape, and both ends thereof are exposed on the upper surface of the flow path member. Further, the bottom of the flow path is open, and this bottom is closed with a metal film. Thereby, a liquid can be made to contact the metal film located in the flow path by sending the liquid into the flow path. When the liquid is fed into the flow path with a pipette, the tip of the pipette is inserted into the end of the exposed flow path, and the sucked liquid is discharged.
Japanese Patent No. 3294605

  However, when inserting the pipette into the flow path, or when pulling out the inserted pipette from the flow path, there is a problem that force is applied from the pipette to the sensor unit and the sensor unit is moved to cause the posture change or positional deviation. there were. Since the detection signal by SPR is very fine, a slight attitude change or positional deviation of the sensor unit also causes a measurement error.

  Such a problem is that when a sensor unit is provided with a plurality of sensor surfaces and corresponding flow paths, and the reaction of the samples on the plurality of sensor faces is to be measured simultaneously, the pipette is naturally pipetted according to the number of flow paths. This is particularly serious because the force acting on the sensor unit becomes larger when the pipette is inserted and removed.

  The present invention has been made in view of the above problems, and an object of the present invention is to provide a measuring apparatus and a sensor unit holding method capable of preventing a change in posture and a positional deviation of the sensor unit that occur when a pipette is inserted and removed. It is.

  The measuring apparatus according to the present invention includes an optical block having a sensor surface formed on the upper surface for detecting a reaction of the sample, and a flow path that is attached to the upper surface and feeds the sample solution containing the sample to the sensor surface. In the measurement apparatus that optically measures the reaction of the sample on the sensor surface by irradiating the optical block with light and receiving the reflected light using a sensor unit comprising a flow path member, the optical block A measuring stage on which the sensor unit is placed in contact with the lower surface of the optical unit, and the sensor unit is detachably set; and the mounting surface formed on the lower ends of both side surfaces of the optical block. And an optical block holding mechanism for holding the sensor unit in a predetermined posture by sandwiching the first and second protrusions protruding in the Y-axis direction substantially parallel to each other from both sides. The optical block holding mechanism has a reference surface that is fixed to a side end of the mounting surface and contacts the first convex portion to position the optical block in the Y-axis direction. A positioning portion that is inclined toward the placement surface, and a pressing surface that contacts and presses the second convex portion, and a pressing position that presses the first convex portion against the reference surface by the pressing surface; And a pressing portion that is displaceable between a release position for retreating from the pressing position and releasing the pressing.

  The measuring device has a pipette that accesses the flow path member along the Z-axis direction from above the sensor unit and has a tip inserted into and removed from the flow path, and the sample solution is inserted into the flow path by the pipette. It is equipped with a dispenser to inject. In the sensor unit, a plurality of sensor surfaces and a plurality of flow paths corresponding to the sensor surfaces are arranged along the longitudinal direction, and a plurality of the sensor units are provided in the dispenser with respect to these flow paths. The pipettes are accessed simultaneously. In such a case, since the force applied to the sensor unit when the pipette is inserted and removed is large, the sensor unit can be reliably held in a stable posture by being held like the optical block holding mechanism.

  The inclination angle of the reference plane is preferably in the range of 20 degrees to 70 degrees. Moreover, it is preferable that the said press surface inclines at the angle which makes the said reference plane symmetrical about the said mounting surface.

  The pressing portion is biased toward the pressing position by a spring, for example. According to this, the holding force can be easily adjusted by adjusting the biasing force of the spring. Further, it is preferable that a plurality of the pressing portions are arranged along the longitudinal direction of the optical block, and at least one is arranged near both ends in the longitudinal direction of the optical block. Furthermore, it is preferable that each said holding | suppressing part can be displaced independently, respectively. Thereby, even when the engagement surface of the optical block is not flat, the holding force can be dispersed. For example, the pressing portion moves up and down along the Z-axis direction and displaces between the pressing position and the release position.

  It is preferable that a cam follower is provided in the pressing portion, and the pressing portion is driven by a cam member having a cam surface serving as an original node with respect to the cam follower. As the cam member, for example, a cam shaft having the cam surface formed on the peripheral surface of the shaft is used, and the cam shaft is rotated to displace the pressing portion. In the camshaft, for example, the cam surface is formed by cutting a part of the peripheral surface of the shaft so that the shaft has a substantially D-shaped cross section.

  Further, as the cam member, a cam plate in which the cam surface is formed by forming a convex portion on a plane is used, and the cam plate is slid to displace the Z-axis direction pressing portion. It may be. The convex portion has, for example, a substantially wedge shape in cross section.

  It is preferable that the cam surface and the cam follower are arranged so that a clearance is secured between the cam surface and the cam follower when the pressing portion reaches the pressing position. Thereby, when the pressing portion is biased by a spring, the biasing force can be converted into a holding force without loss.

  The cam follower is preferably a ball bearing. Thereby, friction with a cam surface is reduced.

  The sensor unit is, for example, transported to the measurement stage along the X-axis direction by combining the X-axis direction orthogonal to the Y-axis direction and the Z-axis direction and the longitudinal direction thereof.

  It is preferable that a stopper for positioning the position in the X-axis direction by contacting the sensor unit that has entered the measurement stage is provided on the mounting surface.

  The reference surface functions as a guide surface that guides the path of the sensor unit in the X-axis direction within the measurement stage.

  It is preferable to provide a flow path pressing mechanism that presses the flow path member against the optical block by accessing the flow path member from above the sensor unit on the mounting surface and pressing the upper surface thereof.

  It is preferable that the flow path pressing mechanism includes, for example, a pressing portion that is movable between a pressing position that presses the flow path member and a retreat position that moves up and retreats from the pressing position. The pressing portion is a pressing plate having a size covering the entire upper surface of the flow path member, and it is preferable that an insertion hole through which the pipette is inserted is formed in the pressing plate.

  It is preferable that the pressing plate is provided with a plurality of plungers on the pressing surface facing the upper surface of the flow path member, and applies a pressing force to the flow path member via these plungers. Moreover, it is preferable that the plurality of plungers are arranged at substantially equal intervals along the longitudinal direction of the pressing plate. The plunger is preferably a ball plunger or a spring plunger.

  The pressing plate preferably presses the flow path member with a thin plate interposed between the plunger and the flow path member. It is preferable that the thin plate is suspended from the pressing plate and moves up and down together with the pressing plate.

  The holding by the optical block holding mechanism and the pressing by the flow path pressing mechanism are preferably started after the conveyance of the sensor unit to the measurement stage is stopped.

  It is preferable that pressing by the flow path pressing mechanism is started after the optical block holding mechanism starts holding. It is preferable to release the pressing by the flow path pressing mechanism after releasing the holding by the optical block holding mechanism.

  The sensor unit holding method of the present invention includes an optical block that is detachably set in a measuring device that optically measures a sample reaction, and has a sensor surface formed on the upper surface to detect the sample reaction, and is attached to the upper surface. In the sensor unit holding method for holding the sensor unit on the measurement stage of the measuring apparatus, a sensor unit comprising a channel member formed with a channel for feeding the sample solution containing the sample on the sensor surface is provided on the optical block. Is provided with first and second convex portions respectively formed at the lower ends of both side surfaces thereof and projecting in the Y-axis direction substantially parallel to the mounting surface, and the sensor unit with the optical block facing downward. A setting step for setting on the mounting surface of the measurement stage, and fixing the Y-axis direction of the optical block by contacting the first convex portion fixed to the side edge of the mounting surface. Having a reference surface to be positioned, and pressing the second convex portion against the positioning portion, the reference surface being inclined toward the placement surface, and pressing the first convex portion against the reference surface And a sandwiching step for sandwiching each convex portion from both sides.

  It is preferable that the second convex portion is pressed by a pressing portion having a pressing surface inclined at an angle symmetrical to the reference surface with the placement surface interposed therebetween.

  According to the present invention, an optical block having a sensor surface for detecting a reaction of a sample and a flow path member formed on the upper surface and having a flow path for feeding a sample solution containing the sample to the sensor surface. A sensor unit having first and second convex portions respectively formed at the lower ends of both side surfaces of the optical block, the sensor unit being placed on the mounting surface of the measurement stage with the optical block facing down. Set, having a reference surface that is fixed to the side edge of the mounting surface and positions the Y-axis direction of the optical block, and the reference surface is inclined toward the mounting surface, By pressing the second convex portion and pressing the first convex portion against the reference surface, the convex portions are sandwiched from both sides. It is possible to prevent.

  As shown in FIG. 1, a sensor unit 11 for detecting the reaction of an analyte analyte is detachably set in the SPR measurement device 10. The SPR device 10 includes a measurement stage 14 on which the sensor unit 11 is set, a measurement unit that measures the reaction of the sample by irradiating the sensor unit 11 with light and receiving the reflected light, and a liquid to the sensor unit 11. A dispensing head 17 having a plurality of pipettes 16 for performing injection and discharge is provided. The measurement unit includes an illumination unit 18 that emits light, and a detector 19 that receives reflected light reflected by the sensor unit 11 and outputs a measurement signal (SPR signal).

  The sensor unit 11 is provided with a flow path member 22 in which a flow path 21 for feeding a liquid is formed, and an optical block that is irradiated with light from the illumination unit 18, reflects the light, and emits reflected light to the detector 19. And a prism 23. The bottom surface of the flow path member 22 is pressed against the top surface of the prism 23. The flow path 21 is a substantially U-shaped liquid feeding pipe, and a facing portion 21a that flows the liquid injected facing the upper surface of the prism 23 along the upper surface of the prism 23, and a flow from both ends of the facing portion 21a. A through portion 21b that penetrates the flow path member 22 in the vertical direction toward the upper surface of the path member 22 is formed. At the upper end of each penetrating portion 21b, an inlet / outlet 21c is formed by inserting the tip of the pipette 16 and serving as a liquid inlet / outlet.

  The two pipettes 16 are interlocked with each other, such that when one is performing an injection operation, the other is performing a suction operation. The liquid injected from the pipette 16 inserted into the one inlet / outlet 21c is discharged from the pipette 16 inserted into the other inlet / outlet 21c.

  The tube diameter of the flow path 21 is, for example, about 1 mm, and the interval between the entrances 21c is, for example, about 10 mm. The facing portion 21 a is a groove formed on the bottom surface of the flow path member 22, and the open portion is covered by the top surface of the prism 23 that is pressed against the bottom surface. The flow path member 22 is formed of an elastic member, and is elastically deformed by pressure contact so that the open portion is sealed.

  On the upper surface of the prism 23, a metal film 24 whose surface becomes the sensor surface 24a is formed by vapor deposition. The metal film 24 has a strip shape and is disposed at a position facing the flow path 21 formed in the flow path member 22. A linker film 26 is formed on the sensor surface 24a. The linker film 26 is a ligand fixing film that fixes a ligand to be brought into contact with the analyte injected into the flow channel 21, and is formed on the metal film 24 when the sensor unit 11 is manufactured. A sensor cell 27 is configured by the sensor surface 24 a including the linker film 26 and the flow path 21.

  On the linker film 26, a ligand is fixed and a measurement region (act region) 26a in which a binding reaction between the analyte and the ligand occurs, and a reference signal for signal measurement in the measurement region is obtained without the ligand being fixed. A reference region (ref region) 26b is formed. The ref region 26b is formed when the linker film 26 is formed. As a formation method, for example, the linker film 26 is subjected to a surface treatment to deactivate a binding group that binds to a ligand in a region about half of the linker film 26. As a result, half of the linker film 26 becomes the act region 26a and the other half becomes the ref region 26b.

  First, a ligand solution containing a ligand is fed to the linker film 26 through the flow path 21, and the ligand is fixed to the act region 26a. Such a fixing process is performed before the sensor unit 11 is set on the measurement stage 14. In the binding reaction between the analyte and the ligand, after the ligand is immobilized, the analyte solution containing the analyte is sent to the linker film 26 through the flow path 21 so that the analyte comes into contact with the immobilized ligand. By detecting the measurement signal at that time, the binding reaction between the ligand and the analyte is measured.

  In the measurement process of the binding reaction between the analyte and the ligand, first, a measurement buffer solution is injected into the channel 21. Thereafter, an analyte solution in which the analyte is dissolved in a solvent is injected, and then the measurement buffer solution is injected again. Note that the flow path 16 may be once cleaned before the measurement buffer solution is first injected. The detection of the SPR signal is started immediately after the measurement buffer solution is first injected in order to detect the reference signal level. After the injection of the analyte solution, the measurement buffer solution is injected again. Done. This makes it possible to detect the reference level (baseline) of the SPR signal, the reaction state of the analyte and the ligand (binding state), and the SPR signal until the desorption of the combined analyte and ligand by injection of the buffer solution for measurement. can do. Injection and discharge of the measurement buffer and the analyte solution are performed by repeatedly inserting and removing the pipette 16 from and into the flow path 21.

  The illumination unit 18 irradiates light through the prism 23 toward the interface between the prism 23 and the metal film 24. As described above, since the reaction state between the ligand and the analyte appears as a change in the resonance angle, the illumination unit 26 causes light beams having various incident angles that satisfy the total reflection condition to enter the interface. The illumination unit 26 includes a light source and an optical system. As the light source, for example, a light emitting element such as an LED (Light Emitting Diode), an LD (Laser Diode), or an SLD (Super Luminescent Diode) is used.

  The optical system includes a collimator lens that collimates light emitted from the light source, an optical fiber that emits parallel light input from the collimator lens as divergent light, and a condensing lens that converges the divergent light emitted from the optical fiber to a specific position on the interface. Etc. Thereby, the light beam of various incident angles is irradiated to the interface. The act region 26a and the ref region 26b are irradiated with light beams (act light and ref light). The act light and the ref light are obtained by, for example, spectrally dividing light from one light source. Generated. Of course, a dedicated light source for each light beam may be provided.

  The detector 19 is composed of, for example, a CCD area sensor or a photodiode array, receives reflected light reflected at the interface, and outputs an electrical signal at a level corresponding to the light intensity of the reflected light as a measurement signal. Since light beams incident at various incident angles are reflected at the interface, the detector 19 receives light beams having various reflection angles corresponding to the light beams. The measurement signal output from the detector 19 is transmitted to a data analysis device (not shown). When the data analyzer performs data processing based on the measurement signal, the light attenuation state is analyzed, and the binding reaction is examined.

  As shown in FIG. 2, the sensor unit 11 has a long shape and is provided with six sensor cells 27. Thus, the processing efficiency of the measurement process is improved by providing a plurality of sensor cells 27 in one unit. This is because if a plurality of sensor cells 27 are provided in one unit, measurement processing for the plurality of sensor cells 27 can be performed with one unit set on the measurement stage 14. For this reason, compared with the case where one sensor cell 27 is provided in one unit, the operation of attaching / detaching the unit to / from the measurement stage 14 is reduced, so that the efficiency of measurement processing is improved. In this example, the number of sensor cells 27 is six, but the number may be other than six, or less or more.

  The SPR measurement device 10 performs measurement processing on the six sensor cells 27 simultaneously with the sensor unit 11 set on the measurement stage 14. The illuminating unit 18 inputs measurement light beams (act light and ref light) simultaneously to the sensor cells 27. The detector 19 simultaneously receives the reflected light from each sensor cell 27 and outputs an SPR signal for each sensor cell 27.

The flow path member 22 has a long rectangular column shape with a square cross section, and is formed of an elastic member. The flow path member 22 includes a main body part 31 in which the flow path 21 is formed and a cover part 32 integrally formed with the main body part 31. Six flow paths 21 are formed in the main body 31, and they are arranged at substantially equal intervals along the longitudinal direction of the main body 31. The bottom surface of the main body 31 is in pressure contact with the top surface of the prism 23.

  As shown in FIG. 3, the cover portion 32 is formed in a substantially U shape so as to cover the upper surface and both side surfaces of the main body portion 31. On the upper surface of the cover portion 32, an entrance / exit 21c of the flow path 21 is formed, and a substantially cylindrical boss 33 protruding upward is formed. Since one sensor cell 27 has two entrances 21c, a total of 12 bosses 33 are arranged on the upper surface of the cover portion 32 along the longitudinal direction. The dispensing head 17 is provided with six sets of two pipettes 16 according to the number of sensor cells 27, for a total of twelve pipettes 16 (see FIG. 4). The dispensing head 17 accesses these bosses 33 from above the sensor unit 11, and its tip is inserted into the inlet / outlet 21 c of the flow path 21.

  A skirt 34 extending downward is formed at both end portions of the cover portion 32. The skirt 34 covers the upper part of both side surfaces of the prism 23. Since the upper part of both side surfaces of the prism 23 is an optical path, if scratches, dust, fingerprints, etc. are attached thereto, irregular reflection of light occurs, leading to deterioration in measurement accuracy. The skirt 34 protects such scratches, dust, fingerprints, and the like from sticking to the prism 23. In the skirt 34, a light transmission window 34 a is formed at a position serving as an optical path for each sensor cell 27, and an engagement hole 34 b for engaging with the prism 23 is formed therebetween.

  For example, the prism 23 has a trapezoidal cross section. Examples of the material of the prism 23 include optical glass such as borosilicate crown (BK7) and barium crown (Bak4), polymethyl methacrylate (PMMA), polycarbonate (PC), and amorphous polyolefin (APO). Representative optical plastics are used. The metal film 24 is formed to be elongated along the longitudinal direction on the upper surface of the prism 23. The linker film 26 is formed corresponding to the position of each flow path 21.

  Engagement protrusions 23 a that engage with the engagement holes 34 b of the flow path member 22 are formed on both side surfaces of the prism 23. The engagement protrusion 23a is disposed at a position corresponding to each engagement hole 34b. The engagement protrusion 23 a and the engagement hole 34 b are engaged, and the flow path member 22 is attached to the prism 23. Further, the prism 23 is formed with a convex portion 23b that protrudes in a substantially horizontal direction from the lower ends of both side surfaces thereof. Each convex portion 23 b has a long plate shape extending in the longitudinal direction of the prism 23. As will be described later, the SPR measurement device 10 is provided with a sensor unit holding mechanism that holds the sensor unit 11 at a predetermined position of the measurement stage 14, and the convex portion 23b is engaged with the sensor unit holding mechanism. Functions as a joint. Further, a groove extending in the longitudinal direction is formed in the center of the width direction on the bottom surface of the prism 23. When the sensor unit 11 is transported to the measurement stage 14, the groove engages with a transport rail (not shown) provided in the SPR measurement device 10 to guide the traveling direction of the sensor unit 11.

  In FIG. 4, the SPR measurement device 10 includes a controller 40 that controls each part of the device. The controller 40 controls the operation and timing of the dispensing head 17, a handling mechanism, a sensor unit holding mechanism described later, and the like.

  The handling mechanism is a transport mechanism that transports the sensor unit 11 to the measurement stage 14. The handling mechanism conveys the sensor unit 11 with its longitudinal direction aligned with the X-axis direction perpendicular to the Z-axis direction parallel to the access direction to the flow path 21 of the pipette 16. The handling mechanism includes a handling head 41 having a pair of arms 41 a that sandwich and hold both ends of the sensor unit 11 in the longitudinal direction. The sensor unit 11 is conveyed and set to the measurement stage 14 while being held by the handling head 41, and measurement processing is performed in that state. Then, after the measurement process is completed, it is transported by the handling head 41 and retracted from the measurement stage 14.

  The measurement stage 14 is provided with a base 42 serving as a stage body, and a mounting table 43 provided on the base 42 and having a long mounting surface 43a on which the sensor unit 11 is mounted. The placement surface 43 a is provided with a stopper 44 that abuts the end of the sensor unit 11 in the longitudinal direction and regulates the amount of entry in the X-axis direction. The stopper 44 positions the sensor unit 11 in the X-axis direction. In addition, the base 42 is provided with a sensor unit holding mechanism that generates a holding force for holding the sensor unit 11 on the mounting surface 43a in a predetermined posture and position to prevent a positional shift or posture change of the sensor unit 11. It has been.

  Since the dispensing head 17 moves up and down along the Z-axis direction and inserts and removes the pipette 16 with respect to the flow path 21, an external force in the Z-axis direction acts on the sensor unit 11 during this insertion and removal. When the positional deviation or posture change of the sensor unit 11 occurs due to such external force in the Z-axis direction, not only the measurement accuracy is deteriorated, but also the handling when the sensor unit 11 is retracted from the measurement stage 14 after the measurement is finished is affected. Effect. Since the SPR measuring device 10 of this example simultaneously performs the measurement of the plurality of sensor cells 27, the pipette 16 is also inserted into and extracted from the plurality of sensor cells 27 at the same time. Compared to the case of measurement, it becomes very large. Therefore, a sufficient holding force to resist the external force is required.

  The sensor unit holding mechanism engages with and holds the prism 23 to hold the sensor unit 11 in a predetermined posture and position, and presses the flow path member 22 from the Z-axis direction. And a flow path pressing mechanism 47 that presses the prism 23 against the prism 23. The prism holding mechanism 46 generates a holding force in the Z-axis direction by engaging with the convex portion 23b of the prism 23, presses the bottom surface of the prism 23 against the mounting surface 43a, and each of the X-axis and the Z-axis. A holding force in the Y-axis direction (the width direction of the prism 23) orthogonal to is generated to position the prism 23 in the width direction.

  After the sensor unit 11 is transported to the measurement stage 14, the controller 40 operates the prism holding mechanism 46 to hold the prism 23. After the prism 23 is held, the controller 40 operates the channel pressing mechanism 47. Then, the flow path member 22 is pressed. When the sensor unit 11 is sent to the measurement stage 14, there is a possibility that the posture of the sensor unit 11 is not properly maintained, for example, the sensor unit 11 is tilted around the X axis. If the flow path member 22 is pressed in a state where the bottom surface of the prism 23 is inclined with respect to the mounting surface 43a, the sensor unit 11 may be further inclined or may fall over if it is severe. For this reason, the prism 23 is held first to correct the posture of the sensor unit 11 to an appropriate posture, and then the pressing by the flow path pressing mechanism 47 is performed.

  Further, after the measurement is completed, the controller 40 releases the holding by the prism holding mechanism 46 and then releases the pressure of the flow path pressing mechanism 47. As will be described later, when releasing the holding by the prism holding mechanism 46, a force acts on the prism 23 in a direction in which the sensor unit 11 is tilted around the X axis. While this force is applied, the pressure by the flow path pressing mechanism 47 is continued to prevent the posture change of the sensor unit 11.

  The prism holding mechanism 46 is driven by a drive mechanism including a motor unit 51 and a camshaft 52. The motor unit 51 transmits the rotational force to the camshaft 52 to drive the prism holding mechanism 46. The camshaft 52 is rotatably attached to a bearing portion formed in a lower portion of the mounting table 43.

  The channel pressing mechanism 47 includes a pressing plate 56 that presses the bottom surface of the channel member 22 against the top surface of the prism 23 by pressing the channel member 22 in the Z-axis direction. The pressing plate 56 is provided so as to be movable up and down in the Z-axis direction, and moves between a pressing position that presses the flow path member 22 against the prism 23 and a retreating position that retreats from the pressing position. The pressing plate 56 has a size that covers the entire upper surface of the flow path member 22. Therefore, the pressing plate 56 is formed with an insertion hole 56a through which the pipette 16 is inserted, and the pipette 16 can access the flow path 21 through the insertion hole 56a.

The pressing plate 56 is driven by a driving mechanism including a motor unit 58, a transmission shaft 59, and a driving member 61. The transmission shaft 59 transmits the rotational force of the motor unit 58 to the drive member 61 via a gear train (not shown). The drive member 61 has a cam follower 62 that engages with a cam surface 64 to be described later attached to the front surface of the drive member 61. When a rotational force is transmitted from the transmission shaft 59, the drive member 61 moves in the horizontal direction (X-axis direction). Slide to. The cam follower 62 is rotatably attached to the drive member 61 in order to reduce frictional resistance with the cam surface 64.

  The pressing plate 56 is held by a holding member 63. The holding member 63 is attached to the base 42 so as to be movable up and down, and moves up and down together with the pressing plate 56. The holding member 63 and the pressing plate 56 are biased upward, that is, in a direction away from the flow path member 22 by tension springs 66 attached to both ends of the pressing plate 56. The pressing plate 56 is maintained in the retracted position in the initial state by this urging.

  As shown in FIGS. 5 and 6, the holding member 63 is provided with a cam surface 64 that engages with the cam follower 62. The cam surface 64 is pressed against the upper cam follower 62 by the urging force of the tension spring 66. The cam surface 64 is an inclined surface, and when the cam follower 62 slides in the horizontal direction, the holding member 63 moves up and down by the inclination of the cam surface 64. Since the cam surface 64 becomes higher from the front right direction toward the left direction, as shown in FIG. 6, when the cam follower 62 slides to the left, the holding member resists the urging force of the tension spring 66. 63 is pushed down along the Z-axis direction. As the holding member 63 is pushed down, the pressing plate 56 moves to the pressing position. On the other hand, when the cam follower 62 slides to the right, the holding member 63 rises along the Z-axis direction by the biasing force of the tension spring 66, and the pressing plate 56 returns to the initial position (retracted position) shown in FIG. To do.

  In the pressing plate 56, a plurality of spring plungers 68 are provided on the lower surface 56 b facing the upper surface of the flow path member 22, and a thin plate 69 is sandwiched between the spring plunger 68 and the flow path member 22, and the flow path member 22. Press. The plurality of spring plungers 68 are fitted into attachment holes 56c formed in the pressing plate 56, and attached so that the tips protrude from the lower surface 56b. The mounting holes 56c are arranged in two rows on both sides along the arrangement direction of the insertion holes 56a. Thereby, the plurality of spring plungers 68 attached to each attachment hole 56c opposes both end portions of the flow path member 22 in the width direction. In addition, the arrangement intervals thereof are substantially equal so that the pressing forces of the plurality of spring plungers 68 are evenly applied along the longitudinal direction.

  As is well known, the spring plunger 68 has a plunger main body, a spring that urges the plunger main body, and a bottomed cylindrical shape that accommodates these and holds the plunger main body so as to be displaceable parallel to the urging direction of the spring. It consists of a case. The plunger body is biased in the initial position so as to be pulled out from the case by a spring bias, and its tip projects from the case. The plunger main body is displaced within a predetermined stroke range from the initial position in the retracting direction sinking into the case. The plunger body has, for example, a substantially bullet shape. Among the spring plungers, there is a ball plunger whose shape of the plunger body is spherical, and such a ball plunger may be used.

  The upper surface of the flow path member 22 has low flatness due to molding accuracy, and moderate distortion occurs. For this reason, if the lower surface 56b of the pressing plate 56 is directly brought into contact with the upper surface of the flow path member 22 and pressed, the pressing force is concentrated on a part according to the strain. I can't apply pressure. Therefore, by pressing the flow path member 22 via each spring plunger 68, the distortion of the upper surface of the flow path member 22 is absorbed by the stroke of the plunger body. Thereby, concentration of the pressing force due to distortion is prevented, and both end portions in the width direction (short direction) of the flow path member 22 are pressed on average along the longitudinal direction.

  However, when the plurality of spring plungers 68 are brought into direct contact with the flow path member 22, the spring plungers 68 and the flow path member 22 are brought into point contact, so that the concentration of the pressing force at each pressing point is concentrated. Inevitable. Therefore, the thin plate 69 is sandwiched and pressed between the spring plunger 68 and the flow path member 22.

  The thin plate 69 is, for example, a metal plate having elasticity. Since the lower surface of the thin plate 69 is brought into contact with the upper surface of the flow channel member 22 and the flow channel member 22 is pressed by the plunger 69 from the upper surface of the thin plate 69, the pressing force of each plunger 69 can be dispersed. By doing so, the pressure applied to the pressing points of the spring plungers 68 can be dispersed while absorbing the distortion of the upper surface of the flow path member 22, and even pressure can be applied along the longitudinal direction of the flow path member 22. Become.

  As shown in FIG. 7, the thin plate 69 has an elongated opening 69a along the longitudinal direction between both end portions facing each spring plunger 68, whereby an access path to the flow path member 22 of the pipette 16 is obtained. Is secured.

  Further, both end portions in the longitudinal direction of the thin plate 69 are bent toward both end portions of the pressing plate 56, and rectangular engagement holes 69b are formed in the bent portions. When the pressing plate 56 is in the retracted position, the thin plate 69 is hooked by the bolts 72 attached to both ends of the pressing plate 56 and suspended from the pressing plate 56. Thereby, the thin plate 69 moves up and down together with the pressing plate 56.

  Further, the engagement hole 69b is formed as a long and elongated hole whose diameter is larger than the diameter of the bolt 72 so that the thin plate 69 can be displaced in the vertical direction even when the bolt 72 is inserted into the engagement hole 69b. Yes. Thereby, the distance between the thin plate 69 and the lower surface 56b of the pressing plate 56 changes between when the pressing plate 56 is in the retracted position (see FIG. 5) and when the pressing plate 56 is in the pressing position (see FIG. 6). Therefore, the expansion and contraction of the spring plunger 68 is not hindered by the thin plate 69.

  That is, as shown in FIG. 5, in a state where the pressing plate 56 is in the retracted position, the thin plate 69 is suspended by being engaged with the bolt 72, so that the distance from the lower surface 56 b of the pressing plate 56 is widened. As a result, a displacement space at the tip of the spring plunger 68 is secured, so that the tip is displaced in the feeding direction without being obstructed by the thin plate 69, and a stroke in the retracted direction is secured. When the pressing plate 56 starts to descend from the retracted position toward the pressing position, first, the thin plate 69 comes into contact with the flow path member 22. At this point, the lowering of the thin plate 69 is finished, but since the size of the engagement hole 69b is larger than the diameter of the bolt 72, the pressing plate 56 reduces the distance from the thin plate 69 even after the displacement of the thin plate 69 is finished. While continuing to descend. In response, the flow path member 22 is pressed through the thin plate 69 while the tip of the spring plunger 68 sinks into the case.

  The prism holding mechanism 46 includes a holding unit 76 and a positioning member 77 arranged on both sides of the mounting surface 43a. The positioning member 77 is fixed to one side end of the placement surface 43 a so as to face the side surface of the prism 23. The positioning member 77 is formed with a reference surface 77 a that contacts the one convex portion 23 b of the prism 23 and positions the prism 23 in the Y-axis direction. The positioning member 77 has an elongated shape along the longitudinal direction of the placement surface 43a, and the reference surface 77a contacts the convex portion 23b of the prism 23 when the sensor unit 11 is conveyed in the X-axis direction. It contacts and functions as a guide rail that guides the traveling direction of the sensor unit 11.

  The holding unit 76 is disposed at a position facing the positioning member 77. The holding unit 76 presses the prism 23 against the positioning member 77 by contacting and pressing the other convex portion 23 b of the prism 23. Thus, the prism 23 is sandwiched from both sides, whereby the sensor unit 11 is positioned in the Y-axis direction and is held on the placement surface 43a in a predetermined posture.

  There are a plurality of holding units 76 and they are arranged at substantially equal intervals along the longitudinal direction of the mounting surface 43a. These holding units 76 are attached to the side surface of the mounting table 43 via an attachment plate 78. As described above, since the sensor unit 11 is provided with the six sensor cells 27, in order to secure the optical path for each sensor cell 27, each holding unit 76 is connected to each other so as to avoid each light transmission window 34 a. It is arranged at a position that does not face. In this example, seven holding units 76 are provided, and five holding units 76 are arranged between the light transmission windows 34a and one each beside the light transmission windows 34a at both ends.

  Each holding unit 76 is provided to be independently displaceable. As a result, even when the protrusion 23 b of the prism 23 is distorted in the longitudinal direction, the distortion is absorbed by the stroke of each holding unit 76, similar to the spring plunger 68 described above. The number of each holding unit 76 does not have to be seven, and it is sufficient if there are two in total, one at each end. The number is appropriately determined in consideration of the number of light transmission windows 34a, the length of the sensor unit 11, and the like.

  The holding unit 76 is fixed by being attached to and fixed to a pressing member 81 having a pressing surface 81a formed in contact with the convex portion 23b and attached to an attachment plate 78, and can be moved up and down in the Z-axis direction. A holding member 82 to be held and a spring 83 that urges the pressing member 81 downward in the Z-axis direction are provided. The pressing member 81 moves up and down between a pressing position and a release position that is lifted from the pressing position and releases the pressing.

  A cam follower 84 that comes into contact with the camshaft 52 is attached to the lower portion of the pressing member 81 with a bolt 85. For example, a ball bearing is used for the cam follower 84, and the cam follower 84 is attached in a direction in which the rotation direction of the cam follower 84 matches the rotation direction of the cam shaft 52. The pressing member 81 is not rigidly fixed to the holding member 82, and is slidably attached. Therefore, the posture is easily changed by an external force. By using a ball bearing for the cam follower 84, the frictional force between the cam shaft 52 and the cam follower 84 is reduced, so that the posture of the pressing member 81 can be suppressed from changing as the cam shaft 52 rotates. Accordingly, an appropriate relative positional relationship between the pressing surface 81a of the pressing member 81 and the convex portion 23b of the prism 23 is maintained. Moreover, the driving torque of the camshaft 52 can be reduced by reducing the frictional force.

  The spring 83 is attached with one end hooked to the clasp 87 and the other end hooked to the clasp 88. The clasp 87 is fixed to the upper surface of the pressing member 81 with bolts 89. The clasp 88 is inserted into the bolt of the mounting plate 78 and fastened with a nut 90 to be fixed.

  The camshaft 52 has a plurality of cam surfaces 91 formed on the peripheral surface of the shaft, and each cam surface 91 is disposed at a position corresponding to each cam follower 84 of the plurality of holding units 76. The cam surface 91 serves as an original node for the cam follower 84, and when the cam shaft 52 rotates, the plurality of holding units 76 are driven by the cam surfaces 91.

  As shown in FIGS. 8 and 9, the cam surface 91 is formed by cutting a part of the peripheral surface of the shaft so that the shaft has a substantially D-shaped cross section, and is called a D-cut cam or the like. . In this example, the D-cut cam is used. However, the D-cut cam may not be used, and a cam having another shape such as a rod-shaped cross section may be used. The cam surface 91 includes a curved surface portion 91a and a flat surface portion 91b. The cam surface 91 is in sliding contact with the cam follower 84 to displace each pressing member 81 in the Z-axis direction. As shown in FIG. 8, while the curved surface portion 91 a and the cam follower 84 face each other, the cam surface 91 keeps the pressing members 81 in the release position against the urging force of the spring 83.

  When the cam shaft 52 rotates clockwise and one end of the flat surface portion (cut portion) 91 b of the cam surface 91 starts to face the cam follower 84, the pressing member 81 is moved by the biasing force of the spring 83. The descent starts along the Z-axis direction. And when the plane part 91b will be in a substantially horizontal state, the pressing member 81 will reach a lower limit (pressing position). Further, when the cam shaft 52 rotates in the clockwise direction, the cam follower 84 and the curved surface portion 91a face each other, and the pressing member 81 returns to the release position shown in FIG.

  In the pressing position, a clearance C is secured between the cam follower 84 and the flat surface portion 91b so as not to contact each other. As a result, the pressing member 81 is suspended from the spring 83 at the pressing position, so that the urging force of the spring 83 can be converted into a pressing force without loss. The biasing force of one spring 83 is, for example, about 500 gf. The stroke amount (distance between the release position and the pressing position) S in which the pressing member 81 is displaced by the urging force of the spring 83 is, for example, about 1.5 mm. The clearance C is, for example, about 0.2 mm to 0.3 mm.

  The reference surface 77a of the positioning member 77 is inclined toward the placement surface 43a. A pressing surface 81a of the pressing member 81 is disposed at a position facing the reference surface 77a with the mounting surface 43a interposed therebetween. The pressing surface 81 and the reference surface 77a face each other in a C shape. In this way, it is inclined at an angle symmetric to the reference plane 77a. For this reason, when the pressing member 81 descends and comes into contact with one convex portion 23b of the prism 23, a force acting on the prism 23 in both the Y-axis direction and the Z-axis direction is generated. Then, the other convex portion 23b of the prism 23 is pressed against the reference surface 77a by the force acting in the Y-axis direction, and positioning in the Y-axis direction is performed. Further, due to the reaction force of the pressing, a force in the Z-axis direction acts on the prism 23 from the reference surface 77a, and the lower surface thereof is pressed against the mounting surface 43a. In this way, the prism 23 is sandwiched between the pair of inclined surfaces (reference surface 77a and pressing surface 81a) facing each other in a C shape, and the holding in the Y-axis direction and the Z-axis direction is performed.

  The sensor unit 11 is transported to the placement surface 43a by the handling head 41, but the posture may be tilted immediately after the transport. Even in such a case, since the holding force in both the Y-axis direction and the Z-axis direction acts on the prism 23 by being sandwiched between the reference surface 77a and the pressing surface 81a, the sensor unit 11 The posture is corrected and held in a proper posture.

  The inclination angle between the reference surface 77a and the pressing surface 81a (the sandwich angle with the mounting surface) is 45 degrees. As a result, the force is evenly distributed in the Y-axis direction and the Z-axis direction. Of course, this inclination angle is an example, and may not be 45 degrees, and may be in the range of 20 degrees to 70 degrees. A preferred range is 30 ° to 60 °, and a more preferred range is 40 ° to 50 °. Further, the inclination angles of the reference surface 77a and the pressing surface 81a are not necessarily the same.

  As described above, the sensor unit holding mechanism holds the sensor unit 11 by the flow path pressing mechanism 47 and the prism holding mechanism 46. Since the flow path pressing mechanism 47 is elastic and presses through the flow path member 22 that is softer than the prism 23, if the flow path pressing mechanism 47 alone has a large force applied to the sensor unit 11 due to insertion and removal of the pipette 16, The deformation of the road member 22 may cause a change in the attitude of the sensor unit 11 or a positional shift. Since the sensor unit 11 is held by the prism holding mechanism 46 that is directly engaged with the prism 23 that is harder than the flow path member 22 in addition to the flow path pressing mechanism 47, the sensor unit 11 can be securely held.

  Hereinafter, the operation of the above configuration will be described with reference to the flowchart shown in FIG. The unprocessed sensor unit 11 is transported in the X direction by the handling head 41 and set on the measurement stage 14. The sensor unit 11 that has entered the measurement stage 14 is guided in the advancing direction by the convex portion 23b on one side contacting the reference surface 77a. When the sensor unit 11 is transported, the prism holding mechanism 46 and the flow path pressing mechanism 47 are in the initial positions shown in FIG. 8, so there is no friction with the pressing surface 81a, and the sensor unit 11 is transported smoothly. When the sensor unit 11 is sent to a position where the end of the sensor unit 11 contacts the stopper 44, the conveyance in the X direction stops.

  After the sensor unit 11 is set on the placement surface 43a, the controller 40 operates the prism holding mechanism 46 and the flow path pressing mechanism 47 at the initial positions shown in FIG. 8 to place the sensor unit 11 at a predetermined position. And hold in posture.

  First, the controller 40 starts the rotation of the camshaft 52 through the motor unit 51 and starts driving the pressing member 81. When the cam surface 91 rotates and contact between the cam follower 84 and the curved surface portion 91 a ends and contact between the cam follower 84 and the flat surface portion 91 b starts, the pressing member 81 moves toward the pressing position by the biasing force of the spring 83. Start to descend. Since a ball bearing is used for the cam follower 84, the frictional resistance between the cam follower 84 and the cam surface 91 is small, and the pressing member 81 can be lowered in a stable posture.

  When the pressing member 81 starts to descend in the Z-axis direction, the pressing surface 81a comes into contact with the corner of the convex portion 23b of the prism 23, and a pressing force in the Y-axis direction is generated by the inclination of the inclined surface. This pressing corrects the posture of the prism 23 while pressing the convex portion 23b on the opposite side of the prism 23 against the reference surface 77a, thereby positioning in the Y-axis direction. The prism 23 is held in both the Y-axis direction and the Z-axis direction by being sandwiched from both sides by a pair of inclined surfaces of the pressing surface 81a and the reference surface 77a.

  Thus, after the prism holding mechanism 46 holds the prism 23, the controller 40 rotates the motor unit 58 to lower the pressing plate 56 toward the pressing position. When the pressing plate 56 is lowered, the thin plate 69 comes into contact with the flow path member 22 and presses the flow path member 22 against the upper surface of the prism 23. Since the pressing plate 56 presses the flow path member 22 through the plurality of plungers 68 and the thin plate 69, the upper surface of the flow path member 22 can be pressed evenly. Further, the pressing by the flow path pressing mechanism 47 is performed after the holding by the prism holding mechanism 46, so that the sensor unit 11 is not tilted or falls by the pressing.

  After the sensor unit 11 is held in this way, the measurement process is started. In the measurement process, a plurality of pipettes 16 access each flow path 21 simultaneously, and liquid is poured into and discharged from each flow path 21, and the SPR signals of the sensor cells 27 are detected simultaneously. Since a plurality of pipettes 16 are inserted and removed at the same time, a large force acts on the sensor unit 11, but the sensor unit 11 is held by the flow path pressing mechanism 47 and the prism holding mechanism 46. There is no change or misalignment.

  The acquisition of the SPR signal is started before the pipette 16 is inserted, and continues for a while after the pipette 16 is pulled out. During this time, since the sensor unit 11 is maintained in an appropriate posture, highly accurate measurement can be performed.

  After the measurement process is completed, the controller 40 first rotates the motor unit 51 to release the holding of the prism holding mechanism 46. At the time of the release, the pressure by the flow path pressing mechanism 47 is continued, so that the posture of the sensor unit 11 is not disturbed when the prism holding mechanism 46 is released. After the prism holding mechanism 46 is released, the pressing plate 56 is raised and the pressing by the flow path pressing mechanism 47 is released. Thereafter, the sensor unit 11 is transported by the handling head 41 and retracted from the measurement stage 14. Since the sensor unit 11 maintains an appropriate posture even after the holding is released, the state in which the sensor unit 11 is securely held by the handling head 41 is maintained. For this reason, a conveyance failure such as the sensor unit 11 falling off the handling head 41 during the conveyance does not occur.

  In the above embodiment, an example in which a camshaft is used as a driving member for driving the pressing member has been described. However, a cam plate 101 as shown in FIGS. 11 and 12 may be used instead of the camshaft. The pressing member 103 is arranged along the longitudinal direction on the side surface of the prism 23, and is disposed to face the positioning member 77. A tongue piece that engages with the convex portion 23 b is formed at the upper part of the pressing member 103, and a cam follower 106 that engages with the cam plate 101 is provided at the lower part.

  The cam plate 101 is arranged with its longitudinal direction aligned with the arrangement direction of the pressing members 103. By sliding in the X-axis direction parallel to the longitudinal direction, the cam plate 101 is driven up and down in the Z-axis direction. Let A rack gear 109 that meshes with the pinion gear 108 is formed at one end of the cam plate 101. When the pinion gear 108 rotates, the cam plate 101 slides along the X-axis direction. The pinion gear 108 is driven by a motor. The cam plate 101 is formed with a cam surface 111 that contacts each cam follower 106.

  As shown in FIG. 12, the cam plate 101 is formed with a plurality of convex portions having a substantially wedge-shaped cross section at a position corresponding to the cam follower 106, and the surface of the convex portions becomes the cam surface 111. The cam surface 111 is formed with an inclined slope portion and a flat flat portion. The pressing member 103 is urged downward by a spring 83 and moves up and down by sliding of the cam surface 111. As indicated by a two-dot chain line, when the flat portion of the cam surface 111 and the cam follower 106 are in contact with each other, the initial position (release position) is established. When the cam plate 101 slides to the right from this initial position, a slope is formed. According to the inclination of the part, the pressing member 103 descends toward the pressing position. When the cam plate 101 is slid in the right direction, the cam surface 111 pushes up the pressing member 103 against the bias of the spring 83 and returns from the pressing position to the initial position.

  In order to apply 100% of the biasing force of the spring 83 to the pressing member 103 at the pressing position, as described above, a clearance is ensured between the cam follower 106 and the cam surface 111. Each should be placed.

  For example, a ball bearing is used for the cam follower 106, and the rotation direction thereof is attached so as to match the sliding direction of the cam plate 101. As a result, the frictional resistance between the cam follower 106 and the cam plate 101 is reduced, which contributes to stabilizing the posture of the pressing member 103 and reducing the driving force of the cam plate 101.

  Compared to the case where the camshaft is used for driving, the case where the cam member is used to drive the pressing member has an effect of reducing the driving force in calculation. Specifically, under the condition that the pressing member biased by the spring with the biasing force of 500 gf is displaced by the stroke amount described above, the driving force when driving with the cam plate is the same as when driving with the camshaft. Calculation results show that only half of the driving force is required.

  In the above-described embodiment, the example in which the pressing member is moved up and down in the Z-axis direction and displaced between the pressing position and the release position has been described. However, the pressing member may not be in the Z-axis direction but may be displaced in the Y-axis direction. May be. In this case, the pressing member comes into contact with the convex portion of the prism from the lateral direction. Further, instead of sliding in the Z-axis direction or the Y-axis direction, the pressing surface may be brought into contact with the convex part by rotating the lower part of the pressing member as a base point.

  In the above embodiment, the pressing member that presses the prism against the inclined reference surface is formed with a pressing surface inclined so as to be symmetric with the reference surface, and the prism is sandwiched between the pair of inclined surfaces. However, for example, if the pressing member 121 is configured as shown in FIG. 13, it is not necessary to provide an inclined pressing surface on the pressing member. In addition, the same code | symbol is attached | subjected for convenience about the same member as the said embodiment.

  The holding unit 122 is provided with a pressing member 121 corresponding to the pressing member 81 of the above embodiment. The pressing member 121 has a tongue piece 121a that comes into contact with the upper surface of the convex portion 23 and presses it in the Z-axis direction, and a Y piece that presses the side surface of the convex portion 23b in the Y-axis direction below the tongue piece 121a. A ball plunger 123 is attached as an axial pressing member. By lowering the pressing member 121 in the Z-axis direction, a pressing force is applied to the convex portion 23a in two directions of the Z-axis direction and the Y-axis direction, and the convex portion 23b on the opposite side of the prism 23 is moved to the reference surface 77a. Can be pressed against.

  As described above, the ball plunger 123 includes a spherical plunger main body (ball), and includes a spring that urges the main body and a case that accommodates these springs. The plunger body is held rotatably about the center of the ball.

  The ball plunger 123 is attached in such a manner that the ball, which is a movable part, faces the side surface of the convex portion 23b so as to be embedded in an attachment hole formed below the tongue piece 121a. As shown in FIG. 13A, the ball plunger 123 is disposed so that the ball slightly protrudes above the convex portion 23b when the pressing member 121 is in the release position.

  Then, as shown in FIG. 13B, when the pressing member 121 is lowered toward the pressing position, the ball first comes into contact with the corner of the convex portion 23b and further descends along the shape of the convex portion 23b. It sinks into the case while being in sliding contact with the peripheral surface, and comes into contact with the side surface of the convex portion 23b. Thereby, pressing in the Y-axis direction is started by the biasing force of the spring of the ball plunger 123. Thus, if the pressing member 121 is configured, it is possible to generate a pressing force in the Z-axis direction and a pressing force in the Y-axis direction, so that the same effect as that of the inclined pressing surface can be obtained.

  Further, since the ball plunger 123 is used as the Y-axis direction pressing member, the ball plunger 123 and the convex portion 23b are prevented from being rubbed and the convex portion 23b is prevented from being scraped. This is because the ball is displaced while being in sliding contact with the convex portion 23b, but rotates at the time of the sliding contact, so that the frictional resistance between the convex portion 23b and the ball is small. For this reason, the convex part 23b is hard to be shaved and generation | occurrence | production of shavings etc. is also suppressed. Note that the use of a ball plunger as the Y-axis direction pressing member has such merits, but a spring plunger whose plunger body does not rotate can be used as the Y-axis direction pressing member instead of the ball plunger. Good.

  In the above-described embodiment, the portion to be engaged with the pressing member is a convex portion protruding from the side surface of the prism, but the convex portion includes irregularities formed by forming a groove in the prism. In the above embodiment, the convex portion of the prism has a substantially rectangular cross section so as to have a substantially horizontal upper surface and a side surface orthogonal thereto, but the shape of the convex portion is not limited to this, for example, The top surface may be inclined or the tip may be dome-shaped.

  In the above embodiment, the SPR measurement device using total reflection attenuation is described as an example of the measurement device. However, the measurement device is not limited to the SPR measurement device, and may be applied to other measurement devices. As a measuring device using total reflection attenuation, for example, a leak mode sensor is known in addition to the SPR measuring device. The leakage mode sensor is composed of a dielectric, and a thin film composed of a clad layer and an optical waveguide layer that are sequentially layered thereon. One surface of the thin film serves as a sensor surface, and the other surface receives light. It becomes a surface. When light is incident on the light incident surface so as to satisfy the total reflection condition, a part of the light is transmitted through the cladding layer and taken into the optical waveguide layer. In this optical waveguide layer, when the waveguide mode is excited, the reflected light at the light incident surface is greatly attenuated. The incident angle at which the waveguide mode is excited changes according to the refractive index of the medium on the sensor surface, similar to the resonance angle of SPR. By detecting the attenuation of the reflection angle, the chemical reaction on the sensor surface is measured.

  In addition, the present invention is not limited to a measurement apparatus that uses total reflection attenuation, but can be applied to other measurement apparatuses that optically measure the reaction of the sensor surface. The shape and the like of the optical block are appropriately changed according to the type of measuring device. For example, although the prism has been described as an example of the optical block, the optical block may not be a prism but may be a plate. The material of the optical block is not limited to plastic but may be glass.

It is explanatory drawing of a SPR measuring method. It is a disassembled perspective view which shows schematic structure of a sensor unit. It is a side view of a sensor unit. It is a block diagram of a measurement stage. It is explanatory drawing of the flow-path pressing mechanism in a retracted position. It is explanatory drawing of the flow-path pressing mechanism in a pressing position. It is a block diagram of a prism holding mechanism. It is explanatory drawing of the prism holding mechanism in a cancellation | release position. It is explanatory drawing of the prism holding mechanism in a pressing position. It is a flow chart which shows a maintenance and release procedure. It is explanatory drawing of the drive mechanism of the pressing member which uses a cam plate. It is a side view of the drive mechanism of FIG. It is explanatory drawing of the example which provided the Y-axis direction pressing member in the pressing member.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 SPR measuring apparatus 11 Sensor unit 14 Measurement stage 16 Pipette 21 Flow path 22 Flow path member 23 Prism 23 Convex part 43 Mounting base 43a Mounting surface 46 Prism holding mechanism 47 Channel pressing mechanism 51 Motor unit 52 Cam shaft 56 Pressing plate 68 Spring plunger 69 Thin plate 76 Holding unit 77 Positioning member 77a Reference surface 78 Mounting plate 81 Holding member 81a Pressing surface 83 Spring 84, 106 Cam follower 91 Cam surface 101 Cam plate

Claims (16)

A sensor comprising an optical block in which a sensor surface for detecting a reaction of a sample is formed on an upper surface, and a flow path member attached to the upper surface and formed with a flow path for feeding a sample solution containing the sample to the sensor surface. In the measuring apparatus that optically measures the reaction of the sample on the sensor surface by irradiating the optical block with light and receiving the reflected light using the unit,
A measurement stage on which the sensor unit is placed in contact with the lower surface of the optical block, and the sensor unit is detachably set;
The sensor unit is held in a predetermined posture by sandwiching first and second convex portions formed at the lower ends of both side surfaces of the optical block and projecting in the Y-axis direction substantially parallel to the mounting surface. An optical block holding mechanism is provided,
The optical block holding mechanism has a reference surface that is fixed to a side end of the mounting surface and contacts the first convex portion to position the Y-axis direction of the optical block. A positioning part inclined toward the surface;
There is a pressing surface that contacts and presses the second convex portion, and a pressing position that presses the first convex portion against the reference surface by the pressing surface, and a release that retreats from the pressing position and releases the pressing A measuring apparatus comprising a pressing portion that is freely displaceable between positions.   The measuring apparatus according to claim 1, wherein an inclination angle of the reference plane is in a range of 20 degrees to 70 degrees. The measuring apparatus according to claim 1, wherein the pressing surface is inclined at an angle symmetric to the reference surface with the placement surface interposed therebetween.

  Dispensing pipette that has access to the flow path member along the Z-axis direction from above the sensor unit and that has a tip inserted into and removed from the flow path, and injects the sample solution into the flow path by the pipette The measuring apparatus according to claim 1, further comprising a measuring device.   In the sensor unit, a plurality of sensor surfaces and a plurality of flow paths corresponding to the sensor surfaces are arranged along the longitudinal direction, and a plurality of pipettes provided in the dispenser with respect to these flow paths. 5. The measuring apparatus according to claim 4, wherein the two are simultaneously accessed.   The measuring device according to claim 1, wherein the pressing portion is urged toward the pressing position by a spring.   A plurality of the pressing portions are arranged along the longitudinal direction of the optical block, and at least one pressing portion is disposed near both ends in the longitudinal direction of the optical block. Or a measuring device.   The measuring device according to claim 7, wherein each of the pressing portions can be independently displaced.   The measuring device according to claim 1, wherein the pressing portion moves up and down along the Z-axis direction and displaces between the pressing position and the release position.   The follower portion is provided with a cam follower, and the retainer portion is driven by a cam member having a cam surface as an original joint with respect to the cam follower. The measuring device described.   The sensor unit is transported to the measurement stage along the X-axis direction by combining the X-axis direction orthogonal to each of the Y-axis direction and the Z-axis direction and the longitudinal direction thereof. The measuring apparatus according to claim 1.   The measurement apparatus according to claim 1, wherein the reference surface functions as a guide surface that guides a path in the X-axis direction of the sensor unit within the measurement stage.   A flow path pressing mechanism for pressing the flow path member against the optical block by accessing the flow path member from above the sensor unit on the mounting surface and pressing the upper surface thereof is provided. The measuring apparatus according to claim 1.   The measurement apparatus according to claim 13, wherein the holding by the optical block holding mechanism and the pressing by the flow path pressing mechanism are started after conveyance of the sensor unit to the measurement stage is stopped. An optical block that is detachably set in a measuring device that optically measures the reaction of the sample and has a sensor surface that detects the reaction of the sample on the upper surface, and a sample that is attached to the upper surface and includes the sample on the sensor surface In a sensor unit holding method for holding a sensor unit including a channel member in which a channel for feeding a solution is formed, on a measurement stage of a measurement device,
The optical block is provided with first and second convex portions respectively formed at the lower ends of both side surfaces thereof and projecting in the Y-axis direction substantially parallel to the mounting surface described above. A set step of setting the sensor unit on the mounting surface of the measurement stage;
It has a reference surface that is fixed to the side edge of the mounting surface and contacts the first convex portion to position the Y-axis direction of the optical block, and this reference surface is inclined toward the mounting surface. A sensor unit comprising: a sandwiching step for sandwiching each convex portion from both sides by pressing the second convex portion against the positioning portion and pressing the first convex portion against the reference surface. Retention method.   16. The method of holding a sensor unit according to claim 15, wherein the second convex portion is pressed by a pressing portion having a pressing surface inclined at an angle symmetrical to the reference surface with the placement surface interposed therebetween. .

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