JP2007145753A - Lipase inhibitor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a lipase inhibitor useful for a food and beverage, a cosmetic or a therapeutic agent for dermatosis. <P>SOLUTION: The lipase inhibitor comprises one or more kinds selected from an extract of Ludwigia octovalvis, an extract of a plant of the genus Pandanus, fruit of Vaccinium membranaceum, fruit of Vaccinium ovatum, fruit of Vaccinium myrtillus and bittern. The decomposition of oils and fats contained in a food and beverage and a cosmetic is prevented through lipase inhibitory activity so as to control an offensive smell, to prevent and treat dermatitis, etc., and to prevent and treat obesity, hyperlipemia, arteriosclerosis, etc. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a lipase inhibitor useful as a food / beverage product, cosmetic product or skin disease therapeutic agent.

  Lipases produced by microorganisms, when mixed in foods and drinks, cosmetics, etc., degrade the contained lipids into glycerin and fatty acids, causing deterioration such as deterioration odor and reducing the value as a product. In addition, lipase produced by microorganisms resident in human skin breaks down the lipids on the skin into glycerin and free fatty acids, and some of these free fatty acids have adverse effects on the skin, such as acne. It is known that it causes skin diseases and free fatty acids are further decomposed to cause body odor. On the other hand, in the human body, lipase is a digestive enzyme secreted from the pancreas, and lipids taken orally are hydrolyzed into glycerin and fatty acids by the action of lipase to promote digestion and absorption in the body. Lipids are particularly high in energy, and fat intake by Japanese in recent years tends to increase, contributing to lifestyle-related diseases such as obesity and hyperlipidemia. In order to solve the problems caused by such lipases, lipase inhibitors that inhibit lipases have been studied. So far, a lipase inhibitor containing alfalfa extract and the like (see Patent Document 1), a lipase inhibitor containing a dihydrochalcone compound (see Patent Document 2), and a lipase inhibitor containing curcumin (see Patent Document 3) Lipase inhibitors containing a specific plant extract (see Patent Document 4) and the like are disclosed.

JP 2003-26585 A Japanese Patent Laid-Open No. 2003-321351 JP 2004-137190 A JP 2005-8572 A

  None of the above known methods are satisfactory in terms of effectiveness and safety, and development of better active ingredients has been demanded. Therefore, this invention is made | formed in view of the said situation, The subject is providing the lipase inhibitor useful for food-drinks, cosmetics, or a skin disease therapeutic agent.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that lipase inhibitory action has been found on the extract of the beetle chicken extract, the extract of the plant of the genus Octopus, the mountain blueberry fruit, the evergreen blueberry fruit, the bilberry fruit, and the bittern. As a result, the present invention has been completed.

  That is, the present invention is characterized in that it contains one or more selected from the extract of the yellowfin bean extract, the extract of the genus Taconoki, the mountain blueberry fruit, the evergreen blueberry fruit, the bilberry fruit, and the bittern. To lipase inhibitors.

  ADVANTAGE OF THE INVENTION According to this invention, the lipase inhibitor useful for food-drinks, cosmetics, or a skin disease therapeutic agent can be provided.

Kidney chicken ( Ludwigia octovalvis var. Sessiliflora ) used as a raw material of the present invention is a perennial plant that grows in a wetland in a warm region and is shrub-like, and has a height of 1 meter. When using this kidney chicken, it is preferable to use an extract. For extraction, any part of plant trunks, branches, fruits, leaves, flowers, seeds, bark, sap, roots, buds, etc. can be used. Use it. In the extraction, the raw material may be used as it is. However, considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably from about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days. Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. And solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia. Extracts from the above-mentioned solvent of Kidney Chicken can be used as they are, but they can be decolorized, deodorized and deodorized as long as they are concentrated and dried, and then dissolved again in water or a polar solvent, or the physiological effects thereof are not impaired. You may use, after refine | purifying a salt etc. or performing the fractionation process by column chromatography etc. The above-mentioned extract of Kidney chicken bait and its processed products and fractions can be lyophilized after each treatment and fractionation and dissolved in a solvent at the time of use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

Next, the genus Pandanus L. f. Used in the present invention is a plant belonging to the family Taconaceae and is widely distributed in tropical and subtropical regions. In Japan, it is found in the Nansei Islands and the like. It grows near the coast, radiates air roots that do not diverge from the woody stem, and has fan-shaped and long leaves. Many species of leaves are used as knitting materials, and there are also species where fruits and seeds are edible. Examples of the genus Pandanus L. f. Plants include Adan ( Pandanus tectorius Soland. Ex Parkins.), Taconoki ( Pandanus boninensis Weber), and Japanese cypress ( Pandanus utilis Bory). In addition to these, Pandanusu Anda imitate Nshi Umm distributed in the Andaman and Nikoman island (Pandanus andamanensium Kurz), distributed in New Guinea Pandanusu Konoidea (Pandanus conoidea Lamck.), Distributed in Madagascar Pandanusu Ejurisu (Pandanus edulis Thouars), distribution in Malaysia Pandanus odorus Ridl. Is known. These extracts of the genus Taconoki can be obtained by a conventional method. For extraction, any part of the trunk, branch, fruit, leaf, flower, seed, bark, sap, root, bud, etc. of the genus Taconoki can be used. Use seeds, whole plants of young trees. In the extraction, the raw material may be used as it is. However, considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably from about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days. Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. And solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia. The above extract of the genus Taconoki can be used as it is, but it can be used again as it is, and it can be dissolved again in water or a polar solvent, or decolorized, deodorized as long as these physiological functions are not impaired. You may use after performing refinement | purification processes, such as desalting, or performing the fractionation process by column chromatography etc. The said extract of a genus Taconoki plant, its processed material, and a fraction can be freeze-dried after each process and fractionation, and it can also be melt | dissolved and used for a solvent at the time of use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

Then, the mountain blueberries (Vaccinium membranaceum) to be used in the present invention, Evergreen blueberries (Vaccinium ovatum), bilberry (Vaccinium myrtillus) is a dicotyledonous plant belonging to the family Ericaceae genus Vaccinium, the fruit is blueberry, huckleberry, bilberry of name It is cultivated for food. Mountain Blueberry and Evergreen Blueberry fruits (collectively abbreviated as “huckleberries” below) and bilberry fruits can be used as they are usually on the market, with fruits that are out of specification due to size, worm-eaten, etc. Also good. Moreover, the fruit juice which squeezed the fruit, its squeezed rice cake, a fruit, fruit juice, and the dried product of the squeezed rice cake can also be used. Furthermore, the extract obtained by extracting with them with a solvent can also be used. The case where it uses as an extract is demonstrated in detail. In the extraction, the raw material may be used as it is. However, considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably from about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days. Extraction solvents for obtaining the extract include water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethyl ether and propyl. Solvents such as ethers such as ether, esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia. Extracts from the above solvents can be used as they are, but the concentrated and dried solids can be dissolved again in water or polar solvents, or decolorized, deodorized, desalted, etc., as long as these physiological functions are not impaired. You may use after performing the refinement | purification process of this, or performing the fractionation process by column chromatography etc. The extract, processed product and fraction thereof can be lyophilized after each treatment and fractionation and dissolved in a solvent before use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

  The bittern used in the present invention is a solution obtained by crystallization of salt from seawater, a dried product thereof, or an artificially mixed mineral. The composition of bittern varies depending on the production area, production method (ion exchange membrane method, sun method, salt field method or evaporation method), temperature at the time of salt crystallization, salt concentration and pressure at the time of salt crystallization, and other conditions. However, bittern prepared using seawater collected in the sea area around the Tokara Islands or in the sea area around Minamidaito Island is particularly preferred because it does not contain contaminants such as organic phosphate compounds contained in the seawater.

  Kidney chicken extract, Taconoki plant extract, mountain blueberry fruit, evergreen blueberry fruit, bilberry fruit, and bitter melon used in the present invention are highly safe lipase inhibitors, food and drink products and skin external preparations And can exert a desired lipase inhibitory action.

  For this reason, it is expected to prevent obesity and hyperlipidemia by daily ingestion of the substance as a food and drink, thereby suppressing the absorption of lipids after ingestion of lipids.

  Moreover, when microorganisms are mixed in foods and drinks and cosmetics containing lipids, it is expected to suppress degradation of lipids by lipase produced by microorganisms and to suppress degradation of lipids such as deteriorated odor.

  Furthermore, on the skin, it is expected to suppress the decomposition of sebum by lipase produced by skin resident bacteria, and to suppress body odor and skin diseases caused by the released fatty acids.

  Lipase inhibitors of the present invention include, for example, fats and oils, margarine, butter, lard, seasonings, creams, dairy products, meat, seafood, eggs, cakes, chocolate, ice cream, coffee, cocoa, tea, milk Can be added to various foods and beverages such as tea, flavor tea, soft drink, carbonated drink, lactic acid bacteria drink, green tea, energy drink, jam, yogurt, manju, jelly, candy, tablet, health food, processed food . In order to finish such a food form, various ingredients commonly used in food according to each purpose, for example, other food raw materials such as sugar, flour, shortening, salt, glucose, chicken egg, chickenpox, calcium, It is possible to use food additives such as nutrient components, emulsifiers, preservatives, etc., but it is particularly preferable to use an amino acid in combination because the lipase inhibitory effect is increased synergistically. The addition amount of the lipase inhibitor at this time is not particularly limited as long as the effect expected by the substance is effectively exhibited, and is 0.00001 to 50% by weight of the total amount, preferably 0.0001 to 10% by weight, more preferably 0.001 to 1% by weight. Further, the lipase inhibitor of the present invention can be ingested in the form of tablets, capsules, granules, liquids and the like.

  Further, the lipase inhibitor of the present invention includes, for example, moisturizing cosmetics, skin roughening cosmetics, makeup cosmetics, anti-dandruff cosmetics, hair growth cosmetics, itching cosmetics, cleaning cosmetics, sunscreen cosmetics, body odor cosmetics, fragrance cosmetics, etc. Can be added to cosmetics. The amount added at this time is not particularly limited as long as the effect expected by the substance is effectively exhibited, and is 0.00001 to 50% by weight of the total amount, preferably 0.0001 to It is 10% by weight, more preferably 0.001 to 1% by weight. In addition, when adding the lipase inhibitor of this invention to cosmetics, another active ingredient, an amino acid, a pharmaceutically acceptable excipient | filler, a pigment | dye, a fragrance | flavor, etc. can also be used in combination as appropriate. Further, the form of the product is also arbitrary, and for example, any of liquid, powder, cream and the like is possible.

  Moreover, the lipase inhibitor of this invention can be added to skin disease therapeutic agents, such as an acne therapeutic agent and a dermatitis therapeutic agent. The amount added at this time is not particularly limited as long as the effect expected by the substance is effectively exhibited, and is 0.00001 to 50% by weight of the total amount, preferably 0.0001 to It is 10% by weight, more preferably 0.001 to 1% by weight. In addition, when adding the lipase inhibitor of this invention to a skin disease therapeutic agent, another active ingredient, an amino acid, a pharmaceutically acceptable excipient | filler, a pigment | dye, a fragrance | flavor, etc. can also be used in combination as appropriate. Further, the form of the product is also arbitrary, and for example, any of liquid, powder, cream and the like is possible.

  Further, the features of the present invention will be described in detail by way of examples. First, it shows about the preparation method of the Kidney chicken extract of this invention.

[Preparation Method 1]
10 kg of 50% by weight ethanol aqueous solution was added to 1 kg of dried pulverized leaves of yellowfin bean and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, a Kidney Chicken extract was obtained.

[Preparation Method 2]
Nine liters of water was added to 1 kg of dried pulverized leaves of yellowfin bean, and the mixture was extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, a Kidney Chicken extract was obtained.

[Preparation Method 3]
Nine liters of methanol was added to 1 kg of dried pulverized leaves of yellowfin bean and soaked at room temperature for 7 days. The extract was collected by filtration, and after removing the solvent, a Kidney Chicken extract was obtained.

[Preparation Method 4]
Kidney chicken leaf was put into a supercritical extraction apparatus and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected to obtain a kidney chicken extract.

  Next, a method for preparing an extract of the genus Taconoki is shown.

[Preparation Method 5]
10 kg of a 50 wt% aqueous ethanol solution was added to 1 kg of a dried pulverized product of the genus Taconoki and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of the genus Taconoki was obtained.

[Preparation Method 6]
Nine liters of water was added to 1 kg of the dried pulverized plant of the genus Taconoki, and the mixture was extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of the genus Taconoki was obtained.

[Preparation Method 7]
Nine liters of methanol was added to 1 kg of the dried pulverized plant of the genus Taconoki and immersed at room temperature for 7 days. The extract was collected by filtration, and after removing the solvent, an extract of the genus Taconoki was obtained.

[Preparation Method 8]
The plant of the genus Taconium was put into a supercritical extraction apparatus and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected to obtain an extract of the genus Octopus.

  Next, methods for preparing mountain blueberry fruits, evergreen blueberry fruits, and bilberry fruits will be described.

[Preparation Method 9]
Mountain blueberry fruit was freeze-dried and ground to obtain a mountain blueberry fruit ground product.

[Preparation Method 10]
Nine liters of water was added to 1 kg of dried crushed fruit of Evergreen blueberry fruit, and the mixture was extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an evergreen blueberry fruit extract was obtained.

[Preparation Method 11]
10 kg of a 50 wt% aqueous ethanol solution was added to 1 kg of dried pulverized bilberry fruit, and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of bilberry fruit was obtained.

  Next, a method for preparing bittern will be described.

[Preparation Method 12] Brine was prepared from seawater collected in the sea area around the Tokara Nigari Tokara Islands using a polyamide composite reverse osmosis membrane. The resulting brine was heated at 110 ° C. to separate it into bittern and salt, thereby obtaining Tokaranigari.

[Preparation Method 13] Brine was prepared from a seawater sampled in the sea area around Minamidaito Island, Nigali Daito Island, using a polyamide composite reverse osmosis membrane. The obtained brackish water was heated at 113 ° C. to separate into bittern and salt, thereby obtaining Minami Daitojima bittern.

[Preparation Method 14] Brine was prepared from seawater collected in the area around the Nigali Doper Strait using the polyamide composite reverse osmosis membrane. The resulting brine was heated at 112 ° C. and separated into bittern and salt to obtain bitter gourd bittern.

[Preparation Method 15] Synthetic bittern sodium chloride (reagent special grade) 5.0 g, potassium chloride (reagent special grade) 3.0 g, magnesium chloride hexahydrate (reagent special grade) 15.0 g, calcium chloride (reagent special grade), 5. Synthetic bittern was obtained by mixing 0 g.

  Next, the evaluation method of the lipase activity inhibitory effect of this invention is shown.

<Evaluation method of lipase activity inhibitory effect>
About Examples 1-6 shown in Table 1, the lipase activity inhibitory effect was evaluated. As a comparative example, a similar test was performed on tetracycline hydrochloride, which has already been reported to have a lipase activity inhibitory effect. Evaluation was made by reacting 4-methylumbelliferyl oleate, which emits fluorescence by deesterification, with lipase (derived from Phycomyces nitens, derived from Propionibacterium acnes ), and producing the generated 4-methylumbelliferone having fluorescence intensity. This was done by quantifying with a meter. Specifically, a lipase solution was prepared to a final concentration of 1 mg / mL in a 96-well microplate, each Example and Comparative Example were added to 0.1 mg / mL, and 4-methylumbellite was further added. Ferryl oleate was added to 100 μM. 4-methylumbelliferone obtained by reacting in the dark at 37 ° C. for 20 minutes and decomposing was measured with a fluorescence intensity meter under conditions of excitation wavelength: 355 nm and fluorescence wavelength: 460 nm. The lipase activity inhibitory effect was determined with the amount of 4-methylumbelliferone produced in the case of reacting only with 4-methylumbelliferyl oleate and lipase (derived from Phycomyces nitens, derived from Propionibacterium acnes ) (control) as 100. It was represented by the amount of 4-methylumbelliferone produced when the example was added. The results are shown in Tables 2 and 3.

  As is clear from Tables 2 and 3, Examples 1 to 6 of the present invention all show a high lipase activity inhibitory effect, and by daily intake of the substance as a food and drink, It is expected to prevent obesity and hyperlipidemia by suppressing the absorption of lipids in the body. Moreover, when microorganisms are mixed in foods and drinks and cosmetics containing lipids, it is expected to suppress degradation of lipids by lipase produced by microorganisms and to suppress degradation of lipids such as deteriorated odor. Furthermore, on the skin, it can be expected that the decomposition of sebum by the lipase produced by the skin resident bacteria is suppressed, and the body odor and skin diseases caused by the released fatty acids are suppressed.

  Next, another embodiment of the present invention will be described.

[Example 7] Lipase inhibiting lotion (1) Ethanol 15.0 (wt%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Lipase inhibitor of the present invention shown in Example 1 of Table 1 0.01
(5) Purified water 100 (6) Citric acid 0.02
(7) Sodium citrate 0.1
(8) Hydroxyethyl cellulose 0.1
Production method: (2) to (4) are dissolved in (1). After dissolution, (5) to (7) are sequentially added, and then sufficiently stirred, (8) is added and mixed uniformly.

[Example 8] Lipase inhibitor emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polystearic acid polyoxyethylene sorbitan (20EO) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxymethyl polymer 0.15
(10) The balance of purified water 100 (11) Arginine (1 wt% aqueous solution) 20.0
(12) Lipase inhibitor of the present invention shown in Example 2 of Table 1 0.5
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification, cooling is started, and (11) and (12) are sequentially added and mixed uniformly.

[Example 9] Lipase inhibiting cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Remainder as purified water 100 (11) Carboxyvinyl polymer (1 wt% aqueous solution) 15.0
(12) Lipase inhibitor of the present invention shown in Example 3 of Table 1 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (11) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, cooling is started, and (12) is added at 40 ° C. and mixed uniformly.

[Example 10] Lipase-inhibiting beverage (1) Lipase inhibitor of the present invention shown in Example 2 in Table 1 1.0 (% by weight)
(2) L-arginine 0.1
(3) Erythritol 1.0
(4) Citric acid 0.1
(5) Remainder manufacturing method to make purified water 100: (1) to (5) are uniformly dissolved.

[Example 11] Candy for lipase inhibition (1) Sucrose 60.5 (wt%)
(2) Remaining as Minamata 100 (3) Lipase inhibitor of the present invention shown in Example 5 of Table 1 0.5
(4) L-isoleucine 1.0
(5) Fragrance 0.1
Production method: (1) to (2) are heated and mixed and homogenized, the components (3) to (5) are added at 60 ° C., and the mixture is homogenized and then molded.

Example 12 Cornflakes for Lipase Inhibition (1) Lipase inhibitor of the present invention shown in Example 6 in Table 1 5.0 (parts by weight)
(2) L-valine 10.0
(3) Corn grits 100.0
(4) Sugar 7.4
(5) Salt 1.0
(6) Malt extract 0.074
(7) Water 30.0
Production method: (1) to (7) are stirred and mixed, then compressed and heated by an extruder, and the resulting dough is crushed and roasted to obtain flakes.

[Example 13] Cookie for inhibiting lipase (1) Fat and oil 170.0 (parts by weight)
(2) Flour 300.0
(3) Lipase inhibitor of the present invention shown in Example 4 of Table 1 2.0
(4) L-tryptophan 10.0
(5) Sugar 50.0
(6) Egg 30.0
(7) Salt 2.0
Production method: (1) to (7) were uniformly mixed to prepare a cookie batter, which was baked at 165 ° C. for 25 minutes to produce a cookie.

The present invention provides a novel lipase inhibitor. According to the lipase inhibitor of the present invention, through the lipase inhibitory action, the decomposition of fats and oils contained in foods and drinks and cosmetics can be prevented, and the malodor of foods and drinks and cosmetics can be suppressed, as well as the lipase inhibitory action. Prevention and treatment of acne, dermatitis, etc., obesity, hyperlipidemia, arteriosclerosis etc. through lipase inhibitory action are possible.

Claims (1)

A lipase inhibitor characterized by containing one or more selected from the extract of the beetle chicken beetle, the extract of the genus Taconoki, the fruit of mountain blueberry, the fruit of evergreen blueberry, the fruit of bilberry, and the bittern.