JP2007031291A - Body weight increase-inhibiting agent and body weight increase-inhibiting drink - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a body weight increase-inhibiting agent and a body weight increase-inhibiting drink that exhibit excellent body increase-inhibiting effects, have no concern about side effects and are excellently safe. <P>SOLUTION: The body weight increase-inhibiting agent and the body weight increase-inhibiting drink contain a Clostridium butyricum culture solution or a Clostridium butyricum culture extract, exhibit excellent body increase-inhibiting effects, have no concern about side effects and are excellently safe. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a weight gain inhibitor and a weight gain inhibitor beverage that are excellent in the effect of suppressing weight gain, have no fear of side effects, and are excellent in safety.

  One of the problems caused by high-calorie diets in modern society is an increase in body weight due to excessive intake of calories, so-called obesity. Obesity resulting from an increase in body weight is not only cosmetically undesirable, but also represents a major health risk that leads to the development of lifestyle-related diseases such as diabetes, hypertension and hyperlipidemia. In addition, weight gain in patients suffering from lifestyle-related diseases such as diabetes, hypertension and hyperlipidemia may induce exacerbation of such diseases. As described above, lifestyle-related diseases and weight gain are closely related, and it is considered that suppressing weight gain is important for suppressing the occurrence of lifestyle-related diseases.

  In order to suppress the increase in weight, it is important to have sufficient exercise and a balanced diet. However, dietary therapy is complicated to calculate calories, and it is difficult to obtain a good effect due to the difficulty of management. On the other hand, suppression of weight gain due to exercise may not provide a sufficient effect. In addition, weight gain is not necessarily caused by environmental factors such as lack of exercise or eating habits, but may be caused by genetic factors. Therefore, drugs that have an action of suppressing weight gain have been developed, which cannot be adequately dealt with only by diet and exercise without bias.

  Patent Document 1 discloses a body weight gain inhibitor comprising a compound having an angiotensin II antagonistic activity, a prodrug thereof, or a salt thereof.

  Patent Document 2 discloses a polypeptide containing a specific amino acid sequence (23 amino acids), an amide or ester thereof, or a salt thereof, a weight gain inhibitor, a weight loss agent, a fat mass increase agent, and a feeding inhibitor. Agents are disclosed.

  On the other hand, in recent years, viable bacteria additives that have beneficial effects on animals by improving the balance of enteric bacteria have attracted attention. These are called probiotics, which increase useful bacteria in the gut and reduce harmful bacteria that make harmful substances. It is only from the viewpoint of preventive medicine, leaving good things for the body and excluding things that are bad for the body, and is different from conventional antibiotic treatment agents that remove good things and bad things for the body. For example, yogurt is mentioned as a probiotic food. Yogurt is a food obtained by fermenting milk with lactic acid bacteria. In addition to nutritional functions, yogurt is known to have various effects such as immunostimulatory action and cancer prevention effect by lactic acid bacteria.

Butyric acid bacterium (Clostridium butyricum) is an obligately anaerobic spore-forming bacterium that produces organic acid and vitamin B group when butyric acid bacteria as probiotics grow in the intestine. It is known to promote the growth of useful bacteria in the intestine such as, and to suppress the growth of harmful bacteria that cause rot and abnormal fermentation to normalize the function of the intestines. It is known that the butyric acid bacterium culture solution or the butyric acid bacterium culture extract remaining after collecting the viable bacteria by filtering the culture solution also has an intestinal flora action (see Patent Document 3). Butyric acid bacteria form spores that can withstand even 100 ° C., and can stop growing under strong acid, strong alkali, or dry conditions, and can start growing again when the environment improves. It can reach the intestines alive. Such a butyric acid bacterium has been used as a medicine or feed for many years, and its safety has been established.
JP 2004-217648 A JP 2004-2295 A JP-A-6-92862

  As described above, various weight gain inhibitors have been developed, but these have the effect of suppressing weight gain, but the effect may not be sufficient. Moreover, since the conventional weight gain inhibitor is a chemically synthesized product, it may not be satisfactory in terms of side effects. For this reason, there is a need for a weight gain inhibitor that is superior in safety and has no fear of side effects.

  Accordingly, an object of the present invention is to provide a weight gain inhibitor that is excellent in the effect of suppressing weight gain, has no fear of side effects, and is excellent in safety.

  As a result of intensive studies in view of the above problems, the present inventors have found a new effect of suppressing weight gain with respect to a butyric acid culture medium or a butyric acid culture extract. Butyric acid bacteria culture solution or butyric acid bacteria culture extract has no fear of side effects and has been known to be excellent in safety. Butyric acid bacteria culture solution or butyric acid bacteria culture extract is excellent in suppressing body weight gain In addition, the present inventors have found that the present invention can be a weight gain inhibitor that is excellent in safety and has no fear of side effects.

  The weight gain inhibitor of claim 1 of the present invention is characterized by containing a culture solution of butyric acid bacteria (Clostridium butyricum) or a butyric acid bacteria culture extract.

  The weight gain inhibitor of Claim 2 of the present invention is the body weight increase inhibitor according to Claim 1, wherein the butyric acid bacterium (Clostridium butyricum) culture solution or butyric acid bacterium culture extract is added to the living bacteria and / or dead cells of the butyric acid bacterium (Clostridium butyricum). It is characterized by adding.

  The weight gain-suppressing beverage according to claim 3 of the present invention is characterized by containing a culture solution of butyric acid bacteria (Clostridium butyricum) or a butyric acid bacteria culture extract.

  According to claim 4 of the present invention, there is provided a beverage for suppressing weight gain according to claim 3, wherein the butyric acid bacterium (Clostridium butyricum) culture solution or butyric acid bacterium culture extract is filled with living and / or dead cells of butyric acid bacteria (Clostridium butyricum). It is characterized by adding.

  According to claim 1 of the present invention, it is effective for suppressing weight gain having a close relationship with lifestyle-related diseases. In addition, butyric acid bacteria as probiotics bring about beneficial effects such as intestinal regulation, antibacterial activity, and digestive aids. Butyric acid bacteria decompose organic substances and produce vitamins and the like, which helps to eliminate the nutritional bias that contributes to obesity and lifestyle-related diseases. Furthermore, since the safety of butyric acid bacteria by oral administration has already been confirmed, it is possible to provide a weight gain inhibitor that is free from side effects.

  According to claim 2 of the present invention, it is further effective in suppressing weight gain having a close relationship with lifestyle-related diseases. In addition, butyric acid bacteria as probiotics bring about beneficial effects such as intestinal regulation, antibacterial activity, and digestive aids. Butyric acid bacteria decompose organic substances and produce vitamins and the like, which helps to eliminate the nutritional bias that contributes to obesity and lifestyle-related diseases. Furthermore, since the safety of butyric acid bacteria by oral administration has already been confirmed, it is possible to provide a weight gain inhibitor that is free from side effects.

  According to claim 3 of the present invention, a butyric acid bacterium (Clostridium butyricum) culture solution or a butyric acid bacterium culture extract that is excellent in the effect of suppressing weight gain, has no fear of side effects, and is excellent in safety is used as a beverage. It can be taken easily. Moreover, this weight gain suppression drink can expect the effect of butyric acid bacteria as probiotics.

  According to claim 4 of the present invention, a culture solution or butyric acid bacterium culture extract that is further excellent in the effect of suppressing weight gain, has no fear of side effects, and is excellent in safety is obtained. Can be taken easily. Moreover, this weight gain suppression drink can expect the effect of butyric acid bacteria as probiotics.

  Hereinafter, embodiments of the present invention will be described in detail.

  The butyric acid bacterium (Clostridium butyricum) used in the present invention is a Gram-positive gonococcus that forms spores with obligate anaerobes. Due to the formation of spores, it has heat resistance and acid resistance, and is highly stable as a living microbe.

  In the present invention, a butyric acid bacterium culture solution is a culture solution obtained by culturing a butyric acid bacterium in a medium containing normal butyric acid bacteria, for example, a medium containing amino acids, potato starch, water, etc. at 20 to 42 ° C. for 20 to 72 hours. I mean.

  Further, in the present invention, the butyric acid bacteria culture extract refers to a liquid part obtained by solid-liquid separation of the above-mentioned butyric acid bacteria culture solution by centrifugation or the like.

  In addition, in the butyric acid bacteria culture solution or butyric acid bacteria culture extract, in addition to live and / or dead cells of butyric acid bacteria, metabolites such as butyric acid, acetic acid, propionic acid and other organic acids, amylase, various amino acids Vitamin B2, Vitamin B6, Vitamin B12, Vitamin C, and self-melting cell components. Moreover, since the butyric acid bacteria culture solution or the butyric acid bacteria culture extract contains butyric acid, acetic acid, or the like as an organic acid, there is a drawback of having a specific odor, but this odor may be masked with a fragrance, for example. The fragrance is not particularly limited as long as it is effective for masking the butyric acid odor, but is preferably used as a food additive.

  Furthermore, the butyric acid bacteria culture solution or butyric acid bacteria culture extract obtained as described above may be used as it is, but can also be used as an extract powder by an operation such as spray drying or freeze drying.

  The viable cell of butyric acid bacterium used in the present invention is not limited at the spore formation stage or the vegetative stage, but is preferably a vegetative stage containing a large amount of peptidoglycan. Viable cells containing abundant vegetative phase are cultured in a PYG medium containing, for example, peptone, yeast extract, glucose, water, etc., and cultured at 20 to 42 ° C. for 20 to 72 hours, and centrifuged or the like. It can be obtained by collecting bacteria.

  Moreover, the dead cell of the butyric acid bacterium used for this invention is obtained by processing a living cell by a normal method, for example using air (oxygen) exposure and heat processing. The viable bacteria for producing dead cells are not limited to those in the spore formation stage or in the vegetative stage, but those in the vegetative stage containing a large amount of peptidoglycan are preferable.

The weight gain inhibitor and weight gain inhibitor beverage of the present invention contain butyric acid bacteria culture solution or butyric acid bacteria culture extract as an active ingredient. Further, it is preferable that the butyric acid bacteria culture solution or butyric acid bacteria culture extract contains 10 ³ / ml or more of butyric acid live cells and / or dead cells.

  In order to formulate the weight gain inhibitor of the present invention, depending on the purpose, administration form, administration target, final form, etc., butyric acid bacteria culture solution or butyric acid bacteria culture extract obtained as described above, or butyric acid bacteria A butyric acid bacterium culture solution or a butyric acid bacterium culture extract containing live cells and / or dead cells is blended with additives such as carriers, excipients, integrating agents, stabilizers, fragrances, etc. as optional components, for example, tablets, Powders, granules, capsules, pills, troches, liquids, extracts and the like can be prepared. These can be prepared according to the usual methods of one skilled in the art. Moreover, you may mix | blend the weight increase inhibitor of this embodiment as it is, or what included it in one part as it is.

In addition, the above-mentioned butyric acid bacteria culture solution or butyric acid bacteria culture extract, or butyric acid bacteria culture solution or butyric acid bacteria culture extract containing live and / or dead cells of butyric acid bacteria is added to the beverage, The weight gain-suppressing beverage of the present invention can be processed and manufactured. Examples of drinks include carbonated drinks, soft drinks, milk drinks, coffee drinks, mineral water, alcoholic drinks, fruit juice drinks, teas, and nutritional drinks. In addition, the above-mentioned butyric acid bacteria culture solution or butyric acid bacteria culture extract, butyric acid bacteria culture solution or butyric acid bacteria culture extract containing live cells and / or dead cells of butyric acid bacteria are added to foods and drinks other than the above beverages, According to this production method, foods and drinks such as candy, rice crackers, cookies, seasonings, rice grains, bread, and noodles can be processed and produced. In addition, it is preferable that the body weight increase inhibitor in the present invention contains 10 ³ cells / ml or more butyric bacteria and / or dead cells.

  The weight gain inhibitor and weight-suppressing beverage of the present invention are not limited to use for beauty, but prevent the occurrence of lifestyle-related diseases such as diabetes, hypertension and hyperlipidemia, and diabetes, hypertension and hyperlipidemia. Can be used to assist in the treatment of lifestyle-related diseases such as illness.

  Hereinafter, the present invention will be described in detail with reference to preparation examples, experimental examples and examples of the present invention, but the present invention is not limited to the following examples.

[Production example] Production method example of butyric acid bacterium culture extract: Butyric acid bacterium (Clostridium butyricum) was added to potato medium [(potato starch 1.0% by weight, amino acid 1.0% by weight, water 98.0% by weight) or (potato Starch 0.5% by weight, amino acid 0.5% by weight, sodium chloride 0.1% by weight, water 98.9% by weight)] and cultured at 20-42 ° C. for 20-72 hours, followed by solid-liquid separation by centrifugation. This liquid part was used as butyric acid bacteria culture extract 1.

  Example of a method for separating vegetative dead cells of a butyric acid bacterium: Clostridium butyricum in a glucose medium (glucose 1.0 wt%, amino acid 1.0 wt%, water 98.0 wt%) The cells were cultured at 42 ° C. for 20 to 72 hours. Next, solid-liquid separation was performed by centrifugation, and the solid was separated into a solid part containing a liquid part and bacterial cells, and the bacterial cells were collected. The cells were sterilized in an autoclave at 100 ° C. or higher for 15 minutes or more to obtain dead vegetative cells.

Further, 10 ³ cells / ml or more of the vegetative dead cells obtained as described above were added to the above-mentioned butyric acid bacteria culture extract 1 to obtain butyric acid bacteria culture extract 2.
[Test Method] Among the NC / Nga TndCrj male 35 day old mice (Nippon Charles River Co., Ltd.), mice judged to be healthy were used in the experiment. These mice were divided into 2 groups so that the body weight was uniform as 6 animals per group. In the extract group, mice were given basic feed for mice and butyric acid bacteria culture extract 2 and the changes in body weight over time were examined. As a control, tap water and basic feed for mice were given to the tap water group instead of butyric acid bacteria culture extract 2, and the change in body weight over time was examined. In addition, refined feed (AIN93G, manufactured by Oriental Yeast Co., Ltd.) was used as a basic feed for mice.

  FIG. 1 is a drawing showing changes in body weight. As shown in FIG. 1, although the same feed was ingested, the extract group had less weight than the tap water group. From this, it became clear that the butyric acid bacteria culture extract 2 has a body weight gain inhibitory effect.

  The example of the weight gain inhibitor of this invention is shown. The butyric acid bacteria culture extract 2 obtained in the production example of Example 1 was dried by irradiation with far-infrared rays under vacuum. The heating temperature is 28-40 ° C. Thereafter, the dried product was pulverized with a crusher, and 150 mg of butyric acid powder was mixed with 150 mg of purified starch powder and 700 mg of lactose to obtain tablets.

  The example of the weight gain suppression drink of this invention is shown. The butyric acid bacteria culture extract 2 obtained in the production example of Example 1 was instantaneously dried with hot air at 150 to 180 ° C. by pressure spraying with a pump. Furthermore, secondary drying was performed at 60 to 85 ° C., and the dried product was pulverized with a crusher to obtain a butyric acid powder. 0.1 g of this butyric acid powder was added to and mixed with 1 liter of 100% apple juice to obtain a juice.

It is a graph showing transition of the weight of each group during an experiment period. In the extract group, the butyric acid bacteria culture extract 2 and the basic feed for mice were given to the mice. The tap water group gave tap water instead of butyric acid bacteria culture extract 2.

Claims (4)

  A weight gain inhibitor comprising a culture solution of a butyric acid bacterium (Clostridium butyricum) or a butyric acid bacterium culture extract.   The body weight gain inhibitor according to claim 1, wherein living and / or dead cells of Clostridium butyricum are added to the culture solution or butyric acid culture extract of the butyric acid bacterium (Clostridium butyricum).   A weight gain-suppressing beverage comprising a culture solution of a butyric acid bacterium (Clostridium butyricum) or a butyric acid bacterium culture extract. 4. The body weight gain-suppressing beverage according to claim 3, wherein living and / or dead cells of butyric acid bacteria (Clostridium butyricum) are added to the culture solution or butyric acid bacteria culture extract of said butyric acid bacteria (Clostridium butyricum).

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