JP2006321791A - Protein-carrying article, protein-carrying filter and method for producing them - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an article carrying a protein such as an enzyme or an antibody which can adsorb and efficiently inactivate various bacteria, molds, viruses, allergen substances and the like. <P>SOLUTION: A surface-treating agent having a composition (alkylene glycol component/hydrocarbon component) mass ratio of 50/50 to 100/0 is used as a substrate to make an enzyme or antibody to effectively act on a filter. A polyester substrate comprising a polyester or amorphous copolyester component is used as a component constituting a single sheet. The protein-carrying article of the present invention can be realized by a simple method in which the substrate is dipped in a protein solution. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

The present invention relates to a carrier and a filter that carry proteins such as enzymes and antibodies that can adsorb and inactivate various bacteria, molds, viruses, allergen substances, and the like. More specifically, a biosensor having a protein supported on a carrier, characterized by specifically stabilizing the activity expression of a protein such as an enzyme or antibody supported by a fiber surface treatment agent and a component of the fiber, The present invention relates to a carrier that can be used for building materials such as wallpaper and curtains that decompose compounds, clothing that decomposes odor components and allergens, and a filter that can be suitably used for air purifier filters and mask filter media.

  Various filter media for removing harmful substances such as various bacteria, molds, viruses, and allergen substances have been proposed so far. However, proteins such as enzymes and antibodies are supported on the filter in a simple manner, and the protein surface treatment agent and the constituent components of the base material supply the water necessary for the expression of the protein activity, making the protein activity expression specific. There has been no proposal of a protein carrier and a filter that can be stabilized stably.

Patent Document 1 discloses a virus removal filter in which at least one of sialic acid, sialic acid derivatives, saccharides containing these, glycoproteins, and glycolipids is a virus trap. Patent Document 2 discloses a fiber material having a high degree of microfibrosis, in which at least one fiber is attached with a natural receptor for virus or a part thereof or an analog thereof for the purpose of capturing the virus. For the purpose of derivatization with cyanogen bromide. However, the method of removing harmful substances in the air by filtration using a filter or physical adsorption using an adsorbent is non-specific and has low accuracy. In addition, in order to prevent the removed harmful substances from re-floating or the growth of harmful substances on the filter to become a new source of contamination, it is necessary to combine techniques for sterilization and inactivation of harmful substances.

Patent Document 3 discloses an antiviral mask composed of a string that holds a non-woven fabric to which tea extract components are attached, and Patent Document 4 discloses a water-insoluble polymer anti-allergen agent having a phenolic hydroxyl group and hygroscopicity. A filter carrying the material is disclosed. Tea extraction components and water-insoluble polymers with phenolic hydroxyl groups are known to have antibacterial activity, but they can inactivate specific fungi and viruses like proteins such as enzymes and antibodies. It has no specific reactivity. Proteins such as enzymes and antibodies are preferred because they can be prepared according to the target harmful substance, and a more reliable effect can be obtained.

In order to sterilize / inactivate harmful substances on the filter, it is effective to support the filter with proteins such as enzymes and antibodies having the ability to sterilize / inactivate harmful substances. However, a filter in which various enzymes are supported on a substrate by a method such as a covalent bond, an ionic bond, a cross-linking method, and a comprehensive method disclosed in Patent Document 5, Patent Document 6, Patent Document 7, and Patent Document 8 is supported. There is a problem that the method is complicated and expensive, and it is desired to carry a protein by a simple method and to express its activity.

Furthermore, Patent Document 4 discloses a method in which a hygroscopic material with a filter surface adsorbs moisture necessary for the expression of the activity of the antiallergen agent from the ambient atmosphere. There is a problem that the amount of protein activity is not always sufficient.

As described above, the protein has superior characteristics compared to the anti-allergen agents in Patent Document 3 and Patent Document 4, that is, the tea extract component and the water-insoluble polymer having a phenolic hydroxyl group. On the other hand, protein expression may be inhibited depending on the hygroscopic material in which the protein coexists. There have been no proposals on base materials and surface treatment agents optimized for protein expression.
JP-A-9-234317 JP 2001-527166 A JP-A-8-333271 JP 2004-290922 A WO98 / 04334 JP 60-49795 A JP-A-2-41166 JP 2003-210919A

The present invention provides a carrier and a filter carrying proteins such as enzymes and antibodies that can adsorb various bacteria, molds, viruses, allergen substances, etc., and inactivate them efficiently with the carrier and filter. It is an object to do.

As a result of intensive studies to improve the performance of protein carriers and protein-carrying filters, the present inventors have found that enzymes, antibodies, and the like can be obtained by coexisting a specific substrate and a surface treatment agent together with proteins such as enzymes and antibodies. The inventors have found that the expression of protein activity is specifically stabilized, and finally completed the present invention. That is, the present invention provides (1) a protein carrier comprising a carrier and a support containing a hydrophilic material supported on a substrate, and (2) the hydrophilic material having a polyalkylene in the molecule. (1) The protein carrier according to (1), characterized by having a glycol component, (3) The amount of the hydrophilic material supported is 0.01% by mass or more based on the base material ( The protein carrier according to 1) or (2), (4) the composition of the hydrophilic material having a polyalkylene glycol component in the molecule is (mass ratio of (alkylene glycol component / hydrocarbon component)) (50/50) To (100/0), the protein carrier according to (2) or (3), and (5) the substrate contains polyester fibers (1) to ( 4) the protein carrier according to any one of ( (2) The protein carrier according to (5), wherein the polyester fiber is a fiber containing an amorphous copolyester component, and (7) the substrate contains silica gel ( The protein carrier according to any one of 1) to (6), (8) the protein carrier according to any one of (1) to (7), wherein the substrate contains activated carbon, (9) A protein-carrying filter comprising the protein-carrying member according to any one of (1) to (8), and the hydrophilic material according to any one of (10), (1) to (3) (1) , (5) to (8) A method for producing a protein carrier, wherein a protein is supported by impregnating a protein solution with a base material.

  The protein carrier and filter of the present invention adsorb various bacteria, molds, viruses, allergen substances, etc. by specifically stabilizing the activity expression of proteins such as enzymes and antibody substances. Can be inactivated efficiently.

  The filter according to the present invention preferably carries a protein. Proteins can be selectively removed by optimizing their types, so there is little influence from the presence of other dust outside the removal object, and the target bacteria etc. can be removed with high accuracy. Because you can.

In addition, tea-extracted components and water-insoluble polymers having phenolic hydroxyl groups have low affinity with water, and when they are attached to the support, they do not mix well, resulting in poor processability. Even if it has an excellent inactivation action such as bacteria, it has been inevitably lowered in performance as a carrier, but proteins such as enzymes and antibodies are of the kind Although there is a difference depending on the type, it generally dissolves well in water, has excellent processability when attached to a substrate, and can be uniformly dispersed throughout the support, improving the ability to remove harmful substances as a support. It also has the effect of stabilizing.

Examples of the protein applicable to the protein carrier of the present invention, such as an antibody, include a lytic enzyme (lysozyme), a proteolytic enzyme (protease), an enzyme that oxidizes and reduces various allergens (oxidoreductase), a bacterium, a mold, Examples include, but are not limited to, antibodies that inactivate viruses, environmental allergens, and the like.

In addition, the enzyme here is “a protein biocatalyst produced in living cells”, and the antibody is “a protein that specifically binds to an antigen produced in vivo by stimulation of an antigen in an immune reaction”. "Protein is a group of high-molecular nitrogen-containing organic compounds contained in cells of animals, plants, microorganisms, and so on that are roughly called living organisms" (Iwanami Biochemical Dictionary 3rd edition Iwanami) Bookstore).

  The carrier according to the present invention preferably carries a hydrophilic material. This is because protein expression can be promoted by supporting the hydrophilic material.

Here, the hydrophilic material used in the present invention refers to a material that retains moisture and can provide moisture and a reaction field necessary for the expression of protein activity, such as polyacrylic acid polymers and polyvinyl alcohol polymers. Examples include molecules and hyaluronic acid, but there is no particular limitation as long as the material has hydrophilicity. A preferable surface treating agent having a polyalkylene glycol component in the molecule is preferable in terms of performance, cost, and processability. That is, the hydrophilic material having a polyalkylene glycol component in the molecule has hydrophilicity and enhances the activity of the protein, and at the same time has excellent affinity with a polymer material such as polyester, A film can be formed uniformly, and the expression of activity can be promoted throughout the protein carried on the substrate.

Surface treatment agents having a polyalkylene glycol component in the molecule include polyalkylene glycol, polyalkylene glycol / alkyl ether, polyalkylene glycol / alkyl phenyl ether, polyalkylene glycol / alkyl ester, polyalkylene glycol / alkyl ether sulfate ester Salt, polyalkylene glycol / alkyl ether phosphate ester salt, polyalkylene glycol / alkyl tertiary amine, polyether / polyester block copolymer containing polyalkylene glycol component, etc. Glycol / alkyl ether is preferable because it has good processability when uniformly applied to the substrate surface. In order to stably express the activity of proteins such as enzymes and antibodies carried on the substrate surface, it is important that the surface treatment agent is uniformly applied to the substrate surface.

Here, the alkylene glycol component refers to ethylene glycol, propylene glycol, butylene glycol, and the like, and ethylene glycol is particularly preferable for use for this purpose because of its high hydrophilicity. The average molecular weight of the polyalkylene glycol is preferably in the range of 100 to 5,000 because of good workability.

Polyalkylene glycol / alkyl ether, polyalkylene glycol / alkyl phenyl ether, polyalkylene glycol / alkyl ester, polyalkylene glycol / alkyl ether sulfate, polyalkylene glycol / alkyl ether phosphate, polyalkylene glycol / alkyl The alkyl group contained in the tertiary amine or the like has a carbon number in the range of 10 to 18, and 12 (lauryl) and 18 (stearyl) are particularly preferable because they are easily available. The amount of this component is defined as the amount of the hydrocarbon component.

The polyether / polyester block copolymer containing a polyalkylene glycol component is a polyether / polyester block copolymer comprising terephthalic acid and / or isophthalic acid, lower alkylene glycol, polyalkylene glycol and / or its monoether. Specifically, terephthalic acid / alkylene glycol / polyalkylene glycol, terephthalic acid / isophthalic acid / alkylene glycol / polyalkylene glycol, terephthalic acid / alkylene glycol / polyalkylene glycol monoether, terephthalic acid / isophthalic acid / alkylene glycol / Examples include polyalkylene glycol monoether. Here, the molar ratio of terephthalic acid to isophthalic acid is preferably in the range of (terephthalic acid / isophthalic acid) = (40/60) to (100/0). The average molecular weight of the block copolymer is usually 2000 to 20000, preferably in the range of 1500 to 8000, and 50% by weight or more is occupied by the polyalkylene glycol component, although it depends on the molecular weight of the alkylene glycol component contained. It is preferable. When the polyalkylene glycol component is less than 50% by weight, the ability to provide moisture and a reaction field necessary for the expression of protein activity is lowered, which is not preferable.

In addition to the surface treatment agent having a polyalkylene glycol component in the molecule, other treatment agents can be used in combination with the substrate of the present invention. However, the hydrocarbon component of the oil agent is hydrophobic and adsorbs to the hydrophobic group of the protein to inhibit its activity, so that the sum of the alkyl glycol components contained in the molecules of all surface treatment agents used The ratio of the sum of the hydrocarbon components is preferably a mass ratio (alkylene glycol component / hydrocarbon component) = (50/50) to (100/0).

However, the preferred range of the mass ratio is (60/40) to (90/10) from the viewpoint of the fiber wetting performance and antistatic performance.

In order to obtain a sufficient effect, the amount of the hydrophilic material supported is preferably 0.01% by weight or more based on the base material. In addition, if it exceeds 50% by weight, even if the amount of adhesion is increased further, the effect of stabilizing and improving the expression of protein activity cannot be expected. On the contrary, the adhesion of the substrate increases, and when processed into a substrate, Hard spots etc. occur, which is not preferable. More preferably, it is 0.05-30 weight%, More preferably, it is 0.1 weight%-10 weight%.

The hydrophilic material may be applied to the carrier either before or after the protein such as an enzyme or an antibody is supported. Specifically, when manufacturing a sheet constituting the substrate, a method of applying to a fiber using a processing roller or the like, or a predetermined surface treatment agent is mixed in a protein solution, and the protein and the surface treatment agent are simultaneously supported. And the like.

The base material of the protein carrier of the present invention preferably contains a polyester fiber, and more preferably the polyester fiber contains an amorphous copolymerized polyester component. Polyester containing an amorphous copolymerized polyester component has good wettability with water, can uniformly carry a hydrophilic material over the entire fiber surface, and has good processability when carrying proteins such as enzymes and antibodies. Because it is good. Furthermore, the polyester containing the amorphous copolymerized polyester component has an appropriate humidity control property. For this reason, the surface treatment agent having a hydrophilic material from the polyester substrate containing the amorphous copolymerized polyester component and the surrounding atmosphere. Absorbs and retains moisture, thereby providing a reaction field for the protein and stabilizing the activity expression, thereby providing a protein-carrying filter that highly stabilizes the protein activity expression. The moderate humidity control here is a performance capable of providing water necessary for the expression of the activity of the protein supported on the surface of the substrate. If the amount of water provided to the protein is too large, the protein will be prematurely deteriorated, and if it is too small, the activity will not be expressed. In addition, the amount of water provided to the protein present on the substrate surface does not have a direct correlation with the water content representing the amount of water present inside the fiber.

The amorphous copolymerized polyester component is preferably obtained by adding a copolymerized component during polyester formation by polycondensation reaction of an aromatic dicarboxylic acid and a glycol component. More preferably, it is based on terephthalic acid and ethylene glycol, and examples of copolymer components include isophthalic acid, adipic acid, sebacic acid, azelaic acid, dodecanoic acid, diethylene glycol, propanediol, butanediol, pentanediol, hexanediol, and polyethylene glycol. And neopentyl glycol. Such an amorphous copolyester has a glass transition point in the range of Tg = 50 to 100 ° C. and does not exhibit a clear crystalline melting point.

In the present invention, it is preferable that the amorphous copolymer polyester component contains terephthalic acid, isophthalic acid, ethylene glycol, diethylene glycol in order to carry a protein such as an enzyme or an antibody substance and stabilize its activity expression. The copolymerization molar ratio is (terephthalic acid / isophthalic acid) = (40/60) to (80/20), and (ethylene glycol / diethylene glycol) = (70/30) to (97/3). More preferably.

The base material containing the amorphous copolymerized polyester component is preferably a composite of the above amorphous copolymerized polyester component and the polyalkylene terephthalate-based polyester component. Specific examples of the polyalkylene terephthalate polyester include polyethylene terephthalate and polybutylene terephthalate. When the substrate is composed of fibers, it is preferably a composite fiber, and forms such as a sheath / core type, an eccentric sheath / core type, and a side-by-side type are possible. Among them, a sheath-core type composite fiber having amorphous copolymer polyester as a sheath component and polyethylene terephthalate as a core component is preferable in terms of processability. The composite ratio of the amorphous copolyester component is 10 to 90%, and preferably 30 to 70% from the viewpoints of performance and workability.

In addition to the above materials, examples of the material for the base material of the protein-carrying filter of the present invention include acrylic, nylon, polyolefin, rayon, cellulose, and pulp, but are not particularly limited.

The fineness of the fiber is preferably adjusted in the range of 1 to 30 dtex from the viewpoints of processability and filter characteristics during sheet formation.

The form of the substrate is a nonwoven fabric such as a film, a spunbond nonwoven fabric, a spunlace nonwoven fabric, a needle punch nonwoven fabric, a melt blown nonwoven fabric, a flash spinning nonwoven fabric, a thermal bond nonwoven fabric, a chemical bond nonwoven fabric, a stitch bond nonwoven fabric, and a wet papermaking nonwoven fabric, or a woven fabric or paper. And the like in the form of a sheet. Moreover, when the form of a base material is a sheet form, the basis weight is not particularly limited, but a preferable range is 10 to 200 g / m ² . If it is less than 10 g / m ² , there is a possibility that inactivated objects such as various bacteria, molds, viruses, and allergen substances cannot be captured sufficiently. On the other hand, if it exceeds 200 g / m ^2, the pressure loss when used as a filter increases, which is not preferable.

As a method for loading a protein such as an enzyme or an antibody substance on a substrate, a method in which the substrate is impregnated with an aqueous protein solution of an appropriate concentration and dried at an appropriate temperature and time is a preferred example. The aqueous protein solution used here is preferably adjusted to a pH suitable for the expression of the activity of each protein. This is because proteins have different pHs suitable for activity expression depending on the type, and normally, sufficient activity expression cannot be obtained unless the pH is around. In addition, it is important that the drying temperature and time are such that the protein carried is not inactivated by denaturation. Proteins vary greatly in heat resistance depending on the type. Usually, drying at a high temperature results in good efficiency after a short time, but it is preferable to determine the drying conditions in consideration of the heat resistance of the protein.

In general, the activity of proteins such as enzymes and antibodies is not expressed in a dry state. Therefore, when a protein such as an enzyme or an antibody is supported on a substrate and used, it is necessary to supply moisture. The inventors of the present invention have developed the activity of the hydrophilic material contained in the surface treatment agent for the fibers constituting the sheet serving as the base material by retaining moisture in the surrounding atmosphere and providing a reaction field for the protein. Found to stabilize. In addition, the base material of the present invention is imparted with an appropriate humidity control action by adding a specific copolymerization component to the inherently hydrophobic polyester, and this stabilizes the protein expression over time. This is because water is also supplied from the polyester base material to the hydrophilic material, and a sufficient amount of water for expression of activity can be supplied to the protein.

  The carrier of the present invention is preferably a filter for the following reasons. In other words, with conventional filters using physical adsorption, it was difficult to remove and decompose low-boiling components, but if a filter carrying a protein that decomposes the target substance is used, even low-boiling components can be removed and decomposed. Is possible. Furthermore, in the conventional filter, there is a problem that trapped bacteria and the like are grown in the filter using the trapped dust at the same time and generate odor or reach the downstream part of the filter to cause secondary contamination. However, this problem can be solved by using a filter carrying a protein that has the effect of killing bacteria.

EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited at all by this.

(Manufacture of base material 1)
As the amorphous copolymer polyester of the sheath component, the acid component is copolymerized at a molar ratio of terephthalic acid of 60%, isophthalic acid of 40%, the diol component of the molar ratio of ethylene glycol of 95% and diethylene glycol of 5%. In addition, amorphous copolyester having an intrinsic viscosity of 0.56 and Tg of 64 ° C., polyethylene terephthalate having an intrinsic viscosity of 0.64, Tg of 67 ° C., and Tm of 256 ° C. as a core component, After drying under reduced pressure, composite spinning with a sheath / core type composite spinning machine, and drawing and cutting with a drawing machine for short fibers, and a composite with a mass ratio (sheath / core) = (50/50) by a conventional method By the ratio, a sheath-core type composite fiber having a single yarn fineness of about 2.2 dtex was obtained. As the hydrophilic material, polyoxyethylene alkyl ether and C12 alkyl phosphate potassium salt are used. The hydrophilic material as a whole has a mass ratio (alkylene glycol component / hydrocarbon component) = (70/30), and the fiber is drawn. It was given when winding later. Here, the hydrophilic material was used in such an amount that 0.1% by mass finally remained with respect to the base material. Next, this fiber was cut into 50 mm, a web was formed by carding, and then a needle punch process was performed to obtain a substrate having a basis weight of 30 g / m ² .

(Manufacture of base material 2)
Polyethylene terephthalate pellets having an intrinsic viscosity of 0.64, Tg of 67 ° C., and Tm of 256 ° C. are dried under reduced pressure, then spun by a single fiber spinning machine, drawn by a single fiber drawing machine, and the single yarn fineness is About 2.2 dtex of polyethylene terephthalate fiber was obtained. As the hydrophilic material, polyoxyethylene alkyl ether and C12 alkyl phosphate potassium salt are used. The hydrophilic material as a whole has a mass ratio (alkylene glycol component / hydrocarbon component) = (70/30), and the fiber is drawn. It was given when winding later. Here, the hydrophilic component was used in such an amount that 0.1% by mass finally remained with respect to the substrate. Next, this fiber was cut into 50 mm, a web was formed by carding, and then a needle punch process was performed to obtain a substrate having a basis weight of 30 g / m ² .

(Manufacture of base material 3)
Polyethylene terephthalate pellets having an intrinsic viscosity of 0.64, Tg of 67 ° C., and Tm of 256 ° C. are dried under reduced pressure, then spun by a single fiber spinning machine, drawn by a single fiber drawing machine, and the single yarn fineness is About 2.2 dtex of polyethylene terephthalate fiber was obtained. The hydrophilic material is a C12 alkyl phosphate potassium salt, and is given when the entire hydrophilic material has a mass ratio (alkylene glycol component / hydrocarbon component) = (0/100) when winding the fiber after stretching. did. Here, the hydrophilic component was used in such an amount that 0.1% by mass finally remained with respect to the carrier sheet. Next, this fiber was cut into 50 mm, a web was formed by carding, and then needle punching was performed to obtain a carrier sheet having a basis weight of 30 g / m ² .

(Enzyme activity test: Test example 1)
Granules freeze-dried with Micrococcus luteus were sprinkled on the protein-carrying filters of Examples and Comparative Examples, and the granules were inverted to remove excess granules, and then allowed to stand in a desiccator maintained at a humidity of 90% for 24 hours. . Then, it observed with the low vacuum scanning electron microscope, and visually observed the grade of the lysis of Luteus bacteria. The case where the sphere shape of the bacterium collapsed was determined to have lytic activity, and the case where the sphere shape remained remained was determined to have no lytic activity.

(Influenza virus inactivation test: Test example 2)
After the protein-carrying filters of Examples and Comparative Examples cut into 33 mm squares were used as test pieces, they were put in sealed containers, and each was sprayed with an aqueous solution containing 0.5 mg of influenza virus per ml into a 0.5 ml container. For 15 hours at room temperature. Thereafter, an aqueous solution containing 1 mg of inactivated influenza virus per 1 ml is sprayed into a 0.5 ml container to block the antibody, and the treated test piece is washed out with 9 ml of a phosphate buffer solution. After inoculation into Japanese eggs and culturing at 37 ° C. for 48 hours, a CAM solution was collected, HA test was performed, and virus infection titer EID ₅₀ (50% egg-infective doses) was calculated by the Karber method.

Example 1
Substrate 1 was dipped in an aqueous solution in which lysozyme lysozyme was suspended in 1% by weight in 100 mM Tris-HCl buffer (pH 8.0) for 1 hour, and then removed to remove excess adhering moisture. The material was dried for 10 minutes in a drier maintained at a temperature of 60 ° C. to prepare a protein-carrying filter.

(Example 2)
A protein-carrying filter was prepared using the substrate 2 in the same process as in Example 1.

(Comparative Example 1)
A protein-carrying filter was prepared using the substrate 3 in the same process as in Example 1.

(Example 3)
An aqueous solution having an influenza virus antibody concentration of 5 mg / 1 ml was prepared using a 100 mM phosphate buffer (pH 7.0), and the substrate 1 was immersed in the aqueous solution for 1 hour, and then removed to remove excess adhering moisture. The substrate was dried for 10 minutes in a drier maintained at a temperature of 60 ° C. to prepare a protein-carrying filter.

Example 4
A protein-carrying filter was prepared using the substrate 2 in the same step as in Example 3.

(Comparative Example 2)
A protein-carrying filter was prepared using the substrate 3 in the same process as in Example 3.

The enzyme activity test shown in Test Example 1 was performed on the protein-carrying filters of Example 1, Example 2, and Comparative Example 1. As a result, in Example 1 and Example 2, the decay of Luteus by the lysing action of lysozyme was confirmed, and in Example 1, the effect was particularly high. In Comparative Example 1, the decay of Luteus was not confirmed. In addition, the influenza virus inactivation test shown in Test Example 2 was performed on the protein-carrying filters of Example 3, Example 4, and Comparative Example 2. As a result, the virus infectivity of Example 3 and Example 4 was EID ₅₀ = 0.5 × 10 ^4.5 , and the virus infectivity of Comparative Example 2 was EID ₅₀ = 0.5 × 10 ^8.5. It was confirmed that the amount of activated virus by the protein-carrying filter of Example 4 was reduced by 4 orders of magnitude, that is, 99.99% inactivation. Table 1 shows the outline of the examples and comparative examples and the comprehensive evaluation results for Test Example 1 and Table 2 for Test Example 2.

  The protein carrier of the present invention realizes high performance because the specific composition of the base material and the surface treatment agent contributes to the expression of the activity of the protein such as the enzyme and antibody supported, Since protein can be loaded by a simple method, it can be easily industrially used.

Claims (10)

A protein carrier comprising a substrate and a carrier containing a protein and a hydrophilic material. The protein carrier according to claim 1, wherein the hydrophilic material has a polyalkylene glycol component in the molecule. The protein carrier according to claim 1 or 2, wherein the amount of the hydrophilic material supported is 0.01% by mass or more based on the base material. The composition of the hydrophilic material having a polyalkylene glycol component in the molecule is (50/50) to (100/0) in mass ratio of (alkylene glycol component / hydrocarbon component). 2. The protein carrier according to 2 or 3. The protein carrier according to any one of claims 1 to 4, wherein the base material contains polyester fibers. 6. The protein carrier according to claim 5, wherein the polyester fiber is a fiber containing an amorphous copolymerized polyester component. The protein carrier according to any one of claims 1 to 6, wherein the substrate contains silica gel. The protein carrier according to any one of claims 1 to 7, wherein the substrate contains activated carbon. A protein-carrying filter comprising the protein-carrying member according to any one of claims 1 to 8. A protein support, wherein the protein is supported by impregnating a protein solution with the base material according to any one of claims 1 to 5 containing the hydrophilic material according to any one of claims 1 to 3. Body manufacturing method.