JP2006124350A - Nmf production promoter - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an NMF (natural moisturizing factor) production promoter promoting the NMF production in an epidermal cell, raising the moisture retention functions of a horny layer and ameliorating drying or pruritus of skin. <P>SOLUTION: This NMF production promoter comprises an extract from one or two or more species selected from Leonurus sibiricus L., Prunus armeniaca L. or Prunus armeniaca L. var. ansu Maximowicz, Lonicera japonica Thinberg, Asiasarum heterotropoides F. or Asiasarum sieboldi F., Angelica sinensis Diels or Angelica acutioba Kitagawa, Coix lacryma-jobi Linne var. ma-yuen Stapf, Prumus persica Batsch or Prumus persica Batsch var. davidana Maximowicz, Rubus suavissimus Shugan Lee and Tremella fuciformis B. The NMF production promoter raises the moisture retention functions of the horny layer and exhibits effectiveness in ameliorating the drying or pruritus of the skin by formulating thereof in a food, a cosmetic, quasi-drug or a medicine, and the like, and promoting the NMF production in the epidermal cell. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a novel NMF production promoter, and in particular, improves the moisturizing function of the stratum corneum by promoting the production of NMF in epidermal cells present in the epidermal granule layer, resulting in dryness-causing, wrinkles, rough skin, etc. The present invention relates to an external preparation for skin.

It has long been known that NMF plays an important role in the moisture retention of the stratum corneum, and so far the NMF component has been applied to the development of moisturizers. The decrease in NMF in the stratum corneum reduces its moisture retention and leads to drying. As a result, dry Kayumi is caused. In recent years, it has been clarified that the amino acid that forms the main component of NMF is produced by the degradation of filaggrin, which is the main component of keratohyalin granules (Non-patent Document 1). That is, profilagrin synthesized in the epidermal granule layer is accumulated in keratohyalin granules and then decomposed into filaggrin through dephosphorylation and hydrolysis in the process from the upper layer of the granule layer to the stratum corneum. Furthermore, filaggrin is decomposed into amino acids in the process of reaching the upper stratum corneum and becomes the main component of NMF. On the other hand, studies on pathophysiology and filaggrin that exhibit dry skin are progressing, and it is known that amino acids are decreased in stratum corneum such as senile xerosis and atopic dermatitis. It has been clarified that the expression of is reduced (Non-Patent Documents 2 and 3). Therefore, in order to increase the production of NMF for the purpose of maintaining the moisturizing property of the stratum corneum, it is considered that the promotion of production of filaggrin or profilagrin in keratinocytes is important, and plant components such as patent documents 1 to 3 are used. Filaggrin or profilagrin production promoters have been reported. On the other hand, NMF production promoting effect, stratum corneum moisturizing function improving effect, skin water retaining ability improving effect and kaymi improving effect of extracts of swordfish, apricot, honeysuckle, saishin, touki, pearl barley, peach, tencha and white jellyfish are known. Absent.
Scott I.D. R. , Biochim. Biophys. Acta. , 719, 110-117 (1982) Tezuka T. Dermatology, 188, 21-24 (1994). Seguchi T. , Arch. Dermatol. Res. , 288, 442-446 (1996). JP 2001-261568 A JP 2002-201125 A JP 2002-363054 A

  As described above, plant components that promote the production of filaggrin or profilagrin, increase the amount of NMF in the stratum corneum, and enhance the moisture retention ability have been proposed, but maintaining a healthy skin state by promoting the production of NMF It is still desirable to provide other substances or compounds that have the effect of

  In view of the above circumstances, the present inventors conducted an sincere study to find an NMF production promoter that promotes NMF production of epidermal keratinocytes. As a result, the present inventors have found that Japanese pheasant, apricot, honeysuckle, saishin, touki, pearl barley, peach, tencha, and white jellyfish. The present invention has been completed by finding that the extract of No. 1 meets the purpose, and further finding out the effect of improving the moisturizing function of the stratum corneum, the effect of improving the water retaining ability of the skin, and the effect of improving the skin.

  That is, the present invention comprises an NMF production promoter, stratum corneum moisturizing agent, characterized by containing one or more extracts selected from swordfish, apricot, honeysuckle, saishin, touki, pearl barley, peach, tencha and white jellyfish The present invention relates to a function improving agent, a skin external preparation for improving water retention ability, and a skin external preparation for improving hair stain.

  The pheasant used in the present invention (scientific name: Leonurus sibiricus L.) is a herb belonging to the genus Lamiaceae, and grows naturally in sunny places in Yamano from Honshu, Shikoku, Kyushu, Korea and China to Southeast Asia. The whole grass of Mejijiki can be used because it is distributed under the name of Yakumosou, and the mature fruit is distributed under the name of Jujushi.

  The apricot used in the present invention is a horn apricot (Prunus armenica L), apricot (Prunus armenica L. var. Ansu Maximowicz) or other related plant belonging to the Rosaceae family, and is widely distributed in China and Japan. A dried seed (herbal medicine name: Anjin) can be used.

  The honeysuckle used in the present invention is a honeysuckle (scientific name: Lonicera japonica Thinberg) belonging to the family Lonicera or other genus plants, and is a creeping plant that grows naturally in Japan, China, and the like. It is possible to use things that are picked and chopped with branches, or leaves that are picked and dried (herbal medicine name: Shinobu) or dried flower parts (herbal medicine name: gold and silver flowers).

  Saisin used in the present invention is Keirinsaicin (scientific name: Asiasarum heterotropoids F.) or Usbasaicin (scientific name: Asiasarum sieboldi F.) belonging to the family Umanosaceae, which is obtained by drying rhizomes and roots, or rooted whole plants ( Can be used.

  The touki used in the present invention is a tomato (scientific name: Angelica sinensis Diels), Toki (scientific name: Angelica acutiba Kitagawa) or other same genus plant belonging to the celery family, dried roots (name of herbal medicine: Toki), etc. Can be used.

  The barley used in the present invention (scientific name: Coix lacryma-jobi Linne var. Ma-yuen Stapf) is a herb belonging to the family Gramineae, and is obtained by drying mature seeds excluding seed coats (herbal medicine name: Yokuinin) can do.

  The peach used in the present invention is a peach (Scientific name: Prumus persica Batsch), a peach (Scientific name: Prumus persica Batsch var davidana Maximovic), or other plants belonging to the same genus. Dry seeds from matured seeds (namely, herbal medicine: peach seed) can be used.

  Tencha (scientific name: Rubus suavissimus Shugan Lee) used in the present invention belongs to the family Rosaceae, is produced only in the mountainous area of southwestern China and is also called open stomach tea and is used as an appetite booster, expectorant, cough . The dried leaves (herbal medicine name: tea) can be used.

  The white jellyfish used in the present invention (scientific name: Tremella fuciformis B.) is a mushroom belonging to the family Asteraceae, and can use fruit bodies, fungus bodies, and the like.

  In addition, for example, the extract of swordfish, apricot, honeysuckle, saishin, touki, pearl barley, peach, tencha, and white jellyfish used in the present invention is soaked or heated with an extraction solvent, filtered, and concentrated if necessary. Obtained. Examples of the extraction solvent include water, lower monohydric alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene). Glycol, etc.), hydrocarbons (hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.), acetonitrile and the like. These solvents may be used alone or in combination of two or more. Preferably, one or two or more of water or a water-soluble solvent (solvent that can be mixed with water in an arbitrary ratio. For example, ethanol, 1,3-butylene glycol, propylene glycol, etc.) may be used. . The extract may be used as it is, or a part or all of the solvent may be distilled off.

  In order to use the NMF production promoter according to the present invention for the above purpose, it is usually administered systemically or locally. Examples of the administration method include oral administration and transdermal administration. The dose varies depending on age, body weight, symptoms, therapeutic effect, administration method, treatment time, etc., but is usually 1 mg to 1 g per adult, preferably 20 mg to 200 mg once to several times a day. Is done. Of course, as described above, since the dosage varies depending on various conditions, an amount smaller than the above-mentioned administration range may be sufficient, or it may be necessary to administer beyond the range.

  In the NMF production promoter of the present invention, the above extract may be used as it is, and a diluent may be used within a range not impairing the effect of the extract. The diluent may be a solid, liquid, or semi-solid, and examples include the following. That is, excipients, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, dispersants, buffers, fragrances, preservatives, solubilizers, solvents, and the like. Specifically, lactose, sucrose, sorbit, mannitol, starch, precipitated calcium carbonate, heavy magnesium oxide, talc, calcium stearate, magnesium stearate, cellulose or derivatives thereof, amylopectin, polyvinyl alcohol, gelatin, surface activity Agents, water, physiological saline, ethanol, glycerin, propylene glycol, cacao butter, laurin butter, petrolatum, paraffin, higher alcohol and the like.

  The NMF production promoter used in the present invention can be used in any of foods, cosmetics, quasi-drugs, and pharmaceuticals. Examples of the dosage form include tablet confectionery, beverages, lotions, creams, emulsions, gels. Agent, aerosol, ointment, poultice, paste, plaster, essence, pack, cleaning agent, bath preparation, foundation, powder, lipstick, powder, pill, tablet, injection, suppository, emulsion, capsule, Granules, liquids (including tinctures, fluid extracts, spirits, suspensions, limonades, etc.).

  The blending amount of the extract of swordfish, apricot, honeysuckle, saishin, touki, pearl barley, peach, tencha and white jellyfish used in the present invention is not particularly limited, but is preferably in the range of 0.0001 to 75% by weight as a dry product. Is 0.001 to 30% by weight. If it is 0.0001% by weight or less, the effect is low, and even if it exceeds 75% by weight, the effect is hardly increased and it is not efficient. The addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.

  The extract of swordfish, apricot, honeysuckle, saishin, pearl oyster, pearl barley, peach, tencha, and white jellyfish of the present invention was excellent in the NMF production promoting effect. Moreover, the external preparation for skin containing one or more of these extracts was safe and excellent in the effect of improving skin dryness and itchiness.

  Next, in order to describe the present invention in detail, examples of production of the extract used in the present invention, formulation examples and experimental examples will be given as examples, but the present invention is not limited thereto. The compounding amount shown in the examples indicates% by weight.

Production Example 1 Mehojiki Ethanol Extract 900 ml of 30% ethanol was added to 100 g of beneficial herb, and extracted at room temperature for 7 days, followed by filtration. The filtrate was concentrated to dryness to obtain 7.5 g of Mejijiki ethanol extract. .

Production Example 2 Apricot ethanol extract 900 mL of 30% ethanol was added to 100 g of apricot kernel, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 5.3 g of apricot ethanol extract. .

Production Example 3 Honeysuckle Ethanol Extract 900 g of gold and silver flowers were added with 900 mL of 30% ethanol, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 5.6 g of honeysuckle ethanol extract. It was.

Production Example 4 Ethanol extract of saicin 900 mL of 30% ethanol was added to 100 g of fine spices, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 4.9 g of saicin ethanol extract. It was.

Production Example 5 Toki Ethanol Extract 900 g of Toki was added with 900 mL of 30% ethanol, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 5.5 g of Toki ethanol extract. It was.

Production Example 6 Ethanol extract of pearl barley 900 mL of 30% ethanol was added to 100 g of Yokuinin, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 5.7 g of pearl barley ethanol extract. .

Production Example 7 Peach ethanol extract 900 mL of 30% ethanol was added to 100 g of peach seed, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 6.5 g of peach ethanol extract. .

Production Example 8 Tencha ethanol extract 900 mL of 30% ethanol was added to 100 g of green tea, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 4.5 g of tencha ethanol extract. .

Production Example 9 Ethanol extract of white jellyfish 900 mL of 30% ethanol was added to 100 g of dry matter of white jellyfish, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain ethanol extract of white jellyfish. 3.4 g was obtained.

Manufacture example 10 Hot water extract of Japanese marlin A 400 ml of purified water is added to 20 g of beneficial mother grass, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate is concentrated and freeze-dried to obtain a hot water extract of Japanese marlin. 2.2 g was obtained.

Production Example 11 Hot water extract of apricot 400 mL of purified water was added to 20 g of apricot kernel, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized to obtain a hot water extract of apricot. 2.5 g was obtained.

Production Example 12 Hot Water Extract of Honeysuckle 400 mL of purified water was added to 20 g of gold and silver flowers, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried, and hot water extract of Honeysuckle. 2.0 g was obtained.

Production Example 13 Hot water extract of saicin 400 mL of purified water was added to 20 g of fine spices, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried, and hot water extract of saicin. 1.5g was obtained.

Production Example 14 Toki Hot Water Extract To 400 g of purified water was added 400 mL of purified water, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried, and hot water extract of Toki. Of 1.8 g was obtained.

Production Example 15 Hot water extract of pearl barley 400 mL of purified water was added to 20 g of Yokuinin, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized to obtain a hot water extract of pearl barley. 2.3 g was obtained.

Production Example 16 Hot water extract of peach 400 mL of purified water was added to 20 g of peach seed and extracted at 95-100 ° C. for 2 hours, followed by filtration. The filtrate was concentrated and freeze-dried to obtain a hot water extract of peach. 1.9 g was obtained.

Production Example 17 Hot water extract of tencha 400 ml of purified water was added to 20 g of tea, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried to obtain hot water extract of tencha. 2.1 g was obtained.

Manufacture example 18 Hot water extract of white jellyfish 2 L of purified water was added to 20 g of dried fruit body of white jellyfish, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated, lyophilized and dried. 1.5 g of a hot water extract was obtained.

Production Example 19 1,3-butyleneglycol aqueous solution extract of Japanese pheasant leek 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of dried whole pheasant grass, extracted at room temperature for 7 days, filtered, 1.9 kg of a 1,3-butylene glycol aqueous extract of pheasantfish was obtained.

Production Example 20 Apricot 1,3-butylene glycol aqueous extract 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of apricot kernel, extracted at room temperature for 7 days, filtered, and apricot 1,3-butylene glycol. 1.9 kg of aqueous butylene glycol extract was obtained.

Production Example 21 1,3-butylene glycol aqueous extract of honeysuckle Add 1 kg of purified water and 1 kg of 1,3-butyleneglycol to 100 g of Shinobifu, extract for 7 days at room temperature, filter, 1.9 kg of aqueous butylene glycol extract was obtained.

Production Example 22 1,3-Butyleneglycol aqueous solution extract of saicin 1 kg of purified water and 1 kg of 1,3-butyleneglycol were added to 100 g of fine spicy, extracted at room temperature for 7 days, filtered, and filtered. -1.9 kg of butylene glycol aqueous solution extract was obtained.

Production Example 23 1,3-butylene glycol aqueous extract of touki 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of Toki, extracted at room temperature for 7 days, filtered, and filtered. -1.9 kg of butylene glycol aqueous solution extract was obtained.

Production Example 24 1,3-butylene glycol aqueous extract of pearl barley 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of yokuinin, extracted at room temperature for 7 days, filtered, and filtered. 1.9 kg of aqueous butylene glycol extract was obtained.

Production Example 25 Extract of 1,3-butylene glycol aqueous solution of peach 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of peach seed, extracted at room temperature for 7 days, filtered, filtered, 1.9 kg of aqueous butylene glycol extract was obtained.

Production Example 26 1,3-Butylene Glycol Aqueous Extract of Tencha 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of green tea, extracted for 7 days at room temperature, filtered, and filtered. 1.9 kg of aqueous butylene glycol extract was obtained.

Production Example 27 1,3-butylene glycol aqueous extract of white jellyfish 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of dried fruit body of white jellyfish, extracted at room temperature for 7 days, filtered, 1.9 kg of a 1,3-butylene glycol aqueous extract of white jellyfish was obtained.

  Next, the prescription of the Example which concerns on this invention is shown.

Formulation Example 1 Cream 1
Formulation amount (% by weight)
1. Shrimp ethanol extract (Production Example 1) 0.5
2. Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5). Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11.1,3-butylene glycol 8.5
12 Ethyl paraoxybenzoate 0.05
13. Methyl paraoxybenzoate 0.2
14 Purified water 67.65
[Production Method] Components 2 to 9 are heated and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Formulation Example 2 Cream 2
In Formulation Example 1, cream 2 was obtained by replacing the peafish ethanol extract with apricot ethanol extract (Production Example 2).

Formulation Example 3 Cream 3
In Formulation Example 1, cream 3 was obtained by substituting the Japanese extract of honeyfish with an extract of honeysuckle (Production Example 3).

Formulation Example 4 Cream 4
In Formulation Example 1, cream 4 was obtained by replacing the ethanol extract of swordfish with the ethanol extract of Saicin (Production Example 4).

Formulation Example 5 Cream 5
In Formulation Example 1, cream 5 was obtained by substituting the ethanol extract of Mejijiki with the ethanol extract of Toki (Production Example 5).

Formulation Example 6 Cream 6
In Formulation Example 1, cream 6 was obtained by replacing the ethanol extract of swordfish with the ethanol extract of pearl barley (Production Example 6).

Formulation Example 7 Cream 7
In Formulation Example 1, cream 7 was obtained by replacing the ethanol extract of eelfish with an extract of peach ethanol (Production Example 7).

Formulation Example 8 Cream 8
In Formulation Example 1, cream 8 was obtained by replacing the ethanol extract of Japanese cypress with the ethanol extract of tencha (Production Example 8).

Formulation Example 9 Cream 9
In Formulation Example 1, cream 9 was obtained by replacing the ethanol extract of swordfish with an ethanol extract of white jellyfish (Production Example 9).

Comparative Example 1 Conventional Cream In Formulation Example 1, a conventional cream was obtained by substituting an ethanol extract of a swordfish with purified water.

Formulation Example 10 Lotion Formulation Amount (% by weight)
1. 1,3-butylene glycol aqueous solution extract of mehajiki (Production Example 19) 0.1
2. 1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5). Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Fragrance 0.1
11. Purified water 84.47
[Production method] Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved, and both are mixed and filtered to obtain a product.

Formulation Example 11 Emulsion Formulation Formulation (wt%)
1. Apricot hot water extract (Production Example 11) 0.05
2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5). Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. Purified water 73.15
[Manufacturing method] Components 2 to 8 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 9 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Formulation Example 12 Ointment Formulation Amount (% by weight)
1. Honeysuckle 1,3-butylene
Glycol aqueous solution extract (Production Example 21) 1.0
2. Polyoxyethylene cetyl ether (30E.O.) 2.0
3. Glycerol monostearate 10.0
4). Liquid paraffin 5.0
5). Cetanol 6.0
6). Methyl paraoxybenzoate 0.1
7). Propylene glycol 10.0
8). Purified water 65.9
[Manufacturing method] Components 2 to 5 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 6 to 8 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled to 30 ° C. with stirring to obtain a product.

Formulation Example 13 Foundation Formulation Amount (% by weight)
1. Saisin 1,3-butylene
Glycol aqueous solution extract (Production Example 22) 1.0
2. Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate 1.0
(20 EO)
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5). Cetanol 1.0
6). Liquid lanolin 2.0
7). Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Butyl paraoxybenzoate 0.1
10. Sodium carboxymethylcellulose 0.1
11. Bentonite 0.5
12 Propylene glycol 4.0
13. Triethanolamine 1.1
14 Methyl paraoxybenzoate 0.2
15. Titanium dioxide 8.0
16. Talc 4.0
17. Bengala 1.0
18. Yellow iron oxide 2.0
19. Fragrance 0.1
20. Purified water 60.0
[Manufacturing method] Components 2 to 9 are heated and dissolved and kept at 80 ° C to obtain an oil phase. Swell component 10 well with component 20, then add components 1 and 11-14 and mix uniformly. To this, components 15 to 18 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The oily phase is added to the aqueous phase with stirring, cooled, and component 19 is added at 45 ° C, and cooled to 30 ° C with stirring to give a product.

Formulation Example 14 Bath preparation formulation Formulation amount (% by weight)
1. Toki hot water extract (Production Example 14) 5.0
2. Sodium bicarbonate 50.0
3. Yellow No. 202 (1) 0.05
4). Fragrance 0.25
5). Anhydrous sodium sulfate 44.7
[Production method] Components 1 to 5 are uniformly mixed to obtain a product.

Formulation Example 15 Tablet Confectionery Formulation Amount (% by weight)
1. Hot water extract of pearl barley (Production Example 15) 2.0
2. Dried corn starch 50.0
3. Erythritol 40.0
4). Citric acid 5.0
5). Sucrose fatty acid ester 3.0
6). Perfume appropriate amount 7. Water Appropriate amount [Production method] Components 1 to 4 and 7 are mixed and granulated. Ingredients 5 and 6 are added to the formed granules and compressed. One tablet is 1.0 g.

Formulation Example 16 Beverage Formulation Amount (% by weight)
1. Peach hot water extract (Production Example 16) 1.0
2. Stevia 0.05
3. Malic acid 5.0
4). Fragrance 0.1
5). [Production method] Components 2 and 3 are dissolved in a small amount of water. Components 1 and 4, 5 are then added and mixed.

Formulation Example 17 Tablet formulation Formulation amount (% by weight)
1. White water jellyfish extract (Production Example 18) 10.0
2. Corn starch 10.0
3. Purified white sugar 20.0
4). Carboxymethylcellulose 10.0
5). Microcrystalline cellulose 35.0
6). Polyvinylpyrrolidone 5.0
7). Talc 10.0
[Production Method] Components 1 to 5 were mixed, and then an aqueous solution of Component 6 was added as a binder and granulated by a conventional method. This was mixed with ingredient 7 as a lubricant and then tableted into 100 mg tablets.

Formulation Example 18 Powder Formulation Amount (% by weight)
1. Tencha hot water extract (Production Example 17) 10.0
2. Corn starch 40.0
3. Microcrystalline cellulose 50.0
[Production method] The above components were mixed and powdered by a conventional method.

Formulation Example 19 Injection formulation Formulation amount (% by weight)
1. Hot water extract of Japanese pheasant (Production Example 10) 1.0
2. Polyoxyethylene (60) hydrogenated castor oil 3.7
3. Sesame oil 0.2
4). Sodium chloride 0.9
5). Propylene glycol 4.0
6). Phosphate buffer (0.1 M, pH 6.0) 10.0
7). Distilled water 80.2
[Production method] Components 2, 3 and half of component 5 are mixed and dissolved by heating at about 80 ° C. To this, component 7 in which components 1 and 4 to 6 are previously dissolved is heated to about 80 ° C and added. The total amount was 1000 mL of an aqueous solution. This aqueous solution was dispensed into 1 mL ampules, melted and then sterilized by heating.

  Next, experimental examples will be given to explain the effects of the present invention in detail.

Experimental Example 1 NMF Production Promotion Test of Keratinocytes Profilagrin mRNA expression level as an index of NMF production of cultured keratinocytes was measured under the following conditions.

Evaluation Method of Effects on Profilaggrin mRNA Expression Mouse keratinocyte-derived Pam212 cells were cultured in Eagle's MEM medium containing 10% FCS under conditions of 37 ° C. and 5% CO ₂ . After becoming confluent, the cells were further cultured for 24 hours in Eagle's MEM medium supplemented with a 0.01 mg / ml sample, and then total RNA was extracted (n = 3). ISOGEN (Nippon Gene) was used for extraction of total RNA. Based on the total RNA extracted from Pam212 cells, the profilagrin mRNA expression level was measured by RT-PCR. The RT-PCR method includes TaKaRa RNA PCR Kit (AMV) Ver. 2.1 and 5′GAATCCATTTTACAGCAAAGCACCTG 3 ′ and 5 ′ GGTATGTCCAAATGTATTGCACGATGTG3 ′ were used as the primers for profilaggrin. GAPDH was used as an internal standard. Other operations were carried out in accordance with a predetermined method, and the PCR reaction solution was subjected to agarose gel electrophoresis, and profilagrin and GAPDH mRNA expression was confirmed as a band. These bands were photographed with a polaroid camera and quantified using a densitometer, and the expression level of profilagrin mRNA was determined as a ratio with respect to the expression level of GAPDH mRNA as an internal standard.

NMF production rate (%) = B / A × 100

A: Profilagrin / GAPDH mRNA expression level when no extract is added B: Profilagrin / GAPDH mRNA expression level when an extract is added

  The test results are shown in Table 1. As a result, excellent NMF production-promoting action was observed in the extracts of swordfish, apricot, honeysuckle, saicin, touki, pearl barley, peach, tencha, and white jellyfish. Moreover, when the extract of the manufacture examples 10-27 was tested, all showed the outstanding NMF production promotion effect | action.

Experimental example 2 Use test Two months use test for 30 women (30-45 years old) suffering from dryness of skin and Kayumi using the creams of prescription examples 1-9 and the conventional cream of comparative example 1, respectively. Went. After use, a questionnaire survey was conducted on the effects of dry skin and improvement of Kayumi. The evaluation criteria of the questionnaire were evaluated as “excellent” for valid, “good” for slightly effective, “good” for slightly effective, and “impossible” for invalid.

  These results are shown in Table 2. The external preparation for skin containing the NMF production promoter of Formulation Examples 1 to 9 showed excellent skin dryness and amelioration effects on Kayumi. During the test period, there was no skin problem and there was no problem with safety.

  When the usage tests of lotions, emulsions, ointments, foundations, bath preparations, tablet confectionery, beverages, tablets and powders of Formulation Examples 10 to 18 were conducted, all showed safe and excellent skin dryness and amelioration effects It was.

As an application example of the present invention, it can be used for any of foods, cosmetics, quasi drugs, and pharmaceuticals. The dosage forms include tablet confectionery, beverages, lotions, creams, emulsions, gels, aerosols, ointments, poultices, pastes, plasters, essences, packs, cleaning agents, bath preparations, foundations, powders, lipsticks. , Powders, pills, tablets, injections, suppositories, emulsions, capsules, granules, liquids (including tinctures, fluid extracts, spirits, suspensions, limonades, etc.) By promoting NMF production in cells, the moisturizing function of the stratum corneum is enhanced, and the effect of improving skin dryness and kayumi is obtained.

Claims (4)

An NMF production promoter characterized by containing one or more extracts selected from mejijiki, apricot, honeysuckle, saishin, touki, pearl barley, peach, tencha and white jellyfish. A stratum corneum moisturizing function-improving agent comprising one or more extracts selected from mejijiki, apricot, honeysuckle, saishin, touki, pearl barley, peach, tencha and white jellyfish. A skin external preparation for improving the water retention ability, comprising one or more extracts selected from mejijiki, apricot, honeysuckle, saishin, touki, pearl barley, peach, tencha and white jellyfish. A skin external preparation for improving oysters, characterized by containing one or more extracts selected from mejijiki, apricot, honeysuckle, saishin, touki, pearl barley, peach, tencha and white jellyfish.