JP2005320281A - Promotor of insulin secretion and food and drink - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a promotor of insulin secretion, and to provide food and drink having little adverse effects, when continuously and perorally administrated/ingested for a long time and effectively improving/treating diabetes mellitus. <P>SOLUTION: The promotor of insulin secretion contains a lower alcohol extract of Butea superba of the family peguminosae as an effective ingredient. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to an insulin secretion promoter and a food or drink containing a specific plant extract as an active ingredient.

  It is estimated that the number of patients with diabetes, a lifestyle-related disease, exceeds 10 million when combined with the reserve army. Diabetes is broadly divided into type I diabetes, in which insulin secretion is impaired due to destruction of β-cells in the pancreas, and type II diabetes, which is mainly caused by insufficient insulin secretion and decreased insulin sensitivity. It is said that there are many type II diabetes. Type II diabetes is a so-called multifactorial disease that occurs when onset factors such as high-fat diet intake, lack of exercise, stress, obesity, etc. have a genetic predisposition and are difficult to treat. .

Dietary therapy, exercise therapy, and pharmacotherapy are used to treat type II diabetes. As therapeutic drugs for diabetes, sulfonylurea (SU agent) that promotes insulin secretion and biguanide, an insulin resistance improving drug. An agent (BG agent) or the like is used (for example, see Non-Patent Document 1).
However, these drugs may cause side effects such as hypoglycemia, vomiting, and abdominal pain, and a therapeutic method having a certain effect has not yet been established.

  It is desirable that the insulin secretagogue is an oral agent that has no side effects and can be taken continuously over a long period of time. From such a viewpoint, a search for a substance having insulin secretion promoting activity from natural products has been advanced, and for example, a white sweet potato extract has been proposed (see, for example, Patent Document 1).

On the other hand, flavonoids extracted from legume butea superba and glycosides thereof are known to suppress the activity of intracellular AMP degrading enzyme phosphodiesterase and have cardiotonic action. However, it is not known about the insulin secretion promoting action.
JP 2002-330726 PR "Diabetes pharmacotherapy", [online], diabetes class, [searched April 6, 2004], Internet <URL: HYPERLINK "http://www.nagara.com/prevent/diabetes/therapy/medication.html "http://www.nagara.com/prevent/diabetes/therapy/medication.html> Roengsumran, S. and 7 others, “Flavonoid and flavonoid glycoside from Butea superba Roxb. And their cAMP phosphodiesterase inhibitory activity” , Journal of the Scientific Research of Chulalongkorn University, (Thailand), 2000, 25, p.169-176

  The present invention has been made in view of such circumstances, and promotes insulin secretion that has few side effects, can be continuously taken or ingested over a long period of time, and can effectively improve diabetes It is an object to provide an agent and food and drink.

  In order to solve the above-mentioned problems, the present inventor has eagerly searched for a substance having an insulin secretion-promoting action in natural products. The present inventors have found that a lower alcohol extract has an excellent insulin secretion promoting activity and completed the present invention.

That is, this invention solves the said subject by the insulin secretion promoter which uses the lower alcohol extract of leguminous plant butea superba (Butea superba) as an active ingredient.
Moreover, this invention solves the said subject by the insulin secretion promoter which uses the ketone solvent soluble fraction in the lower alcohol extract of the leguminous plant Butea superba (Butea superba) as an active ingredient.
Furthermore, this invention solves the said subject by the food-drinks characterized by containing these insulin secretion promoters and having attached | subjected the indication that it was used for insulin secretion promotion.

  The insulin promoter of the present invention has an excellent insulin secretion promoting action, has few side effects, and is excellent in safety. Can also be taken.

  Butea superba used in the present invention is a kind of tropical plant inhabiting the Mekong River basin in Thailand.

  Butea sperba extract is obtained by subjecting the root of butea sperva to pretreatment such as drying, cutting, pulverization, and pulverization as necessary, and then extracting with a lower alcohol. In that case, it is preferable to use dry root powder. The extraction conditions are not particularly limited, but it is preferable to mix 0.5 to 5 kg of butea sperba root with 1 to 15 L of lower alcohol and extract under reflux for 1 to 24 hours. It is efficient to repeat the operation of fractionating the lower alcohol after the completion of the first extraction and further adding and extracting the lower alcohol a plurality of times.

  Examples of the lower alcohol include alcohols having 1 to 5 carbon atoms, specifically, methanol, ethanol, propanol, isopropanol, butanol and the like, and ethanol is most preferable.

  The lower alcohol extract of Butea sperva is used as an extract extracted by concentrating or distilling off the lower alcohol as necessary.

Furthermore, when the extract of the lower alcohol of Butea sperba is subjected to silica gel column chromatography and the fractions eluted and fractionated with various solvents are used, the ketone solvent-soluble fractions can be used to enhance insulin secretion. Is particularly preferable.
Examples of the ketone solvent include acetone, methyl ethyl ketone, methyl propyl ketone, isopropyl methyl ketone and the like, and acetone is particularly preferable.

The lower alcohol extract of Butea sperva produced in this way has an excellent insulin secretion promoting action and few side effects, and is extremely useful as an agent for improving / treating diabetes.
In order to obtain an insulin secretagogue, a pharmaceutically acceptable carrier such as an excipient, a lubricant, a diluent, a binder, a disintegrant, an emulsifier, a stabilizer, a miso odorant, etc. is used as appropriate. Thus, it is preferable to prepare a preparation for oral administration such as tablets, capsules, granules, powders, syrups and the like.

  The dosage of the lower alcohol extract of Butea sperva of the present invention is preferably 1 mg to 3 g / day (adult), more preferably 2 mg to 1.5 g / day (adult) as the extract. It is preferable to administer this usually in 3-4 times a day.

The lower alcohol extract of Butea sperba of the present invention may be blended in foods and drinks, and is particularly preferably a health food and drink that promotes health.
For such foods and drinks, food additives such as preservatives, colorants, sweeteners, antioxidants, thickening stabilizers, emulsifiers, seasonings, preservatives, natural products, and the like can be used. The form is not particularly limited, and any of drinks, tablets, powders, granules, pastes, and the like may be used. In particular, a beverage is preferable.

  EXAMPLES Next, although an Example is given and this invention is demonstrated concretely, this invention is not limited to these Examples.

Example 1 Preparation of Extract Extract and Ketone Solvent-Soluble Fraction 5 kg of Thai leguminous plant Butea sperva root dry powder was put into 8 L of ethanol and extracted for 10 hours under ethanol reflux. Sorted. The operation of mixing and extracting the separated butea sperba root powder with 8 L of ethanol was repeated three times. In all ethanol parts, ethanol was distilled off to obtain 27 g of an extract from Butea sperva.
27 g of ethanol extract was subjected to silica gel column chromatography, 0.3 g of extract was extracted in 900 mL of hexane (F1), 2 g of extract was extracted in 1300 mL of chloroform (F2), and 800 mL of acetone was eluted (F3). 10 g of the extract and 10 g of extract in the fraction eluted with 600 mL of methanol (F4). Each elution fraction was dissolved in dimethyl sulfoxide (DMSO) after distilling off the solvent.

Test example 1
The insulin secretion promoting activity of the acetone-eluted fraction (F3) of butea sperva obtained in Example 1 was measured.

Action on isolated pancreatic cells
The method of Yamada et al. (Endocrinology, 143, 4203-4209 (2002)) was partially modified. The pancreas was removed from normal rats, digested with collagenase, filtered and washed repeatedly to obtain cell pellets. Acetone-eluted fraction (F3) in the amount shown in Table 1 was dissolved in 0.5% DMSO in 1 mL of a solution containing 10 ⁴ cells in Krebs-Ringer bicarbonate buffer (KRBG) containing 2.8 mM glucose. 5 μl of each sample was added, incubated for 60 minutes at 37 ° C., washed, and 1 mL of KRBG solution containing protease inhibitor cocktail (1 tablet / 50 mL), 11.1 mM glucose and 50 mM KCl was added to stimulate glucose. After incubation for 30 minutes, the mixture was centrifuged, and each insulin contained in the supernatant was quantified by enzyme immunoassay (EIA method) using a rat insulin measurement kit (Amersham Pharmacia Biotech). In addition, what added 5 microliters of purified water instead of the test substance was set as control. The results are shown in Table 1 as a percentage (%) relative to the control.
As apparent from Table 1, the acetone-eluting fraction (F3) of Butea sperva exhibited an excellent insulin secretion promoting action.

Action on cultured islets Measurement was performed using an assay kit (manufactured by Wako Pure Chemical Industries, Ltd.) in which only islets were isolated and cultured from normal rat pancreas. 0.5 mL of the sample was added to a solution containing 10 islets in 0.2 mL of basal media, incubated for 60 minutes, washed, and 0.5 mL of glucose-stimulating medium containing the sample was further added. After incubation for 60 minutes in the presence of 17 mM glucose, insulin contained in the supernatant was quantified by enzyme immunoassay (EIA method) using a rat insulin measurement kit (Amersham Pharmacia Biotech). In the same manner as described above, a sample to which 5 μl of purified water was added instead of the sample was used as a control. The results are shown in Table 2 as a percentage (%) relative to the control.
As apparent from Table 2, the acetone-eluted fraction (F3) of Butea sperva exhibited an excellent insulin secretion promoting action.

Example 2 Beverages The following processed milk was produced.
Milk 100 parts by weight Butea Superba ethanol extract 0.002 parts by weight

Example 3 Drinks The following drinks were produced.
mg / 100mL
Butea Superba ethanol extract 2
Oligosaccharide 80
Amount of citric acid pH 3.0-3.5 Aspartame 10
Vitamin C 20
Fructose 1700
100 mL of purified water

Claims (5)

  An insulin secretagogue comprising as an active ingredient a lower alcohol extract of the legume plant Butea superba.   The insulin secretion promoter according to claim 1, wherein the lower alcohol is ethanol.   An insulin secretagogue comprising as an active ingredient a ketone solvent-soluble fraction in a lower alcohol extract of the legume butea superba.   The insulin secretion promoter according to claim 3, wherein the lower alcohol is ethanol.   A food or drink comprising the insulin secretagogue according to any one of claims 1 to 4, and a label indicating that the insulin secretagogue is used for promoting insulin secretion.