JP2005281159A - Composition having hypotensive activity obtained from barley shochu distillation liquid residues - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition having significant hypotensive activity using barley Shochu (white distilled liquor) distillation liquid residues as a byproduct from Shochu production process using barley as feedstock. <P>SOLUTION: This composition having the significant hypotensive activity comprises a desorption fraction that is obtained by the following process: Barley Shochu distillation liquid residues as a byproduct from Shochu production process using barley as the feedstock are subjected to solid/liquid separation to obtain a liquid fraction, which is then subjected to adsorption treatment using a synthetic adsorbent to obtain a synthetic adsorbent adsorption fraction, which, in turn, is eluted with an alkali or ethanol. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  In the present invention, a barley shochu distillation residue obtained as a by-product in the production of shochu using barley as a raw material is subjected to solid-liquid separation to obtain a liquid component. It is related with the composition which has a blood pressure lowering effect which consists of a desorption fraction obtained by obtaining a fraction and eluting this synthetic adsorbent adsorption fraction using an alkali or ethanol.

It is known that the barley shochu distillation residue produced as a by-product when producing barley shochu has the following pharmacological action. That is, it has been reported that the barley shochu distillation residue suppresses lipid accumulation in the rat liver by administration of orotic acid (for example, Non-Patent Document 1, Japanese Society of Nutrition and Food Science, Abstracts of the Annual Meeting, Vol. .53, 53 (1999)).
The barley shochu distillation residue has the above fatty liver inhibitory action, which is stronger than that of wine lees and beer lees, and this action is not observed at all in shochu distillate. Because of its small size, it is reported that it is unique only to the barley shochu distillation residue (see, for example, Journal of Japan Brewing Association, Vol. 94, No. 9, 768 (1999) as Non-Patent Document 2). ).
In addition, the barley shochu distillation residue has an onset-inhibiting action against D-galactosamine-induced liver damage, which is known to exhibit the same symptoms as viral hepatopathy, and the onset-inhibiting action is the barley shochu distillation residue. It has been reported that the liquid content obtained by subjecting the liquid to centrifugation (see, for example, Journal of the Japan Brewing Society, Vol. 95, No. 9, 706 (2000) as Non-Patent Document 3). .
Furthermore, it has been reported that a fiber component composed of barley groove contained in the barley shochu distillation residue exhibits a blood pressure lowering effect (for example, Japan Food Science, Vol. 42, No. 1 as Non-Patent Document 4). , 34 (2003)).
Abstracts of Annual Meeting of Japanese Society of Nutrition and Food Science, Vol.53, 53 (1999) Journal of Japan Brewing Association, Vol.94, No.9, 768 (1999) Journal of Japan Brewing Association, Vol.95, No.9, 706 (2000) Japan Food Science, Vol.42, No.1, 34 (2003)

In JP-A-2001-145472 as Patent Document 1, a barley shochu distillation residue obtained as a by-product in the production of shochu using barley as a raw material is subjected to solid-liquid separation to obtain a liquid component, and an alkali is added to the liquid component. An alkali-soluble fraction, neutralized the alkali-soluble fraction with an acid to obtain a neutral soluble fraction, and an organic acid fractionated by adding ethanol to the neutral soluble fraction, It is described that a composition comprising an ethanol-insoluble fraction containing protein and hemicellulose has an inhibitory effect on orotic acid-induced liver injury in an experiment using rats.
Japanese Patent Application Laid-Open No. 2003-38158 as Patent Document 2 discloses that a barley shochu distillation residue obtained as a by-product in the production of shochu using barley as a raw material is subjected to solid-liquid separation to obtain a liquid, and the liquid is used as a synthetic adsorbent. An orotic acid-induced fatty liver and / or D-galactosamine-induced hepatitis comprising an adsorbed fraction obtained by subjecting to an adsorption treatment to be used, and a desorbed fraction obtained by eluting the adsorbed fraction with an alkali or ethanol A purified concentrate having the action of inhibiting the development of is described.
Japanese Patent Application Laid-Open No. 2003-73294 as Patent Document 3 discloses that a barley shochu distillation residue obtained as a by-product in the production of shochu using barley as a raw material is subjected to solid-liquid separation to obtain a liquid, and an organic solvent is added to the liquid A leukemia cell growth inhibitor comprising an organic solvent-insoluble fraction fractionated by this method is described.
Japanese Patent Application Laid-Open No. 2003-73295 as Patent Document 4 discloses that a barley shochu distillation residue obtained as a by-product in the production of shochu using barley as a raw material is subjected to solid-liquid separation to obtain a liquid, and an organic solvent is added to the liquid A natural killer cell activator consisting of an organic solvent-insoluble fraction fractionated by doing so is described.
Japanese Patent Laid-Open No. 2001-145472 Japanese Patent Laid-Open No. 2003-38158 JP 2003-73294 A JP 2003-73295 A

  Regarding the pharmacological action of the barley shochu distillation residue, as described above, Non-Patent Document 4 describes that the fiber component composed of barley groove contained in the barley shochu distillation residue exhibits a blood pressure lowering action. . On the other hand, in Non-Patent Document 1 to Non-Patent Document 3, a liquid component obtained by subjecting barley shochu distillation residue or barley shochu distillation residue to solid-liquid separation to remove the fiber component comprising the barley groove is described. (Hereinafter referred to as the liquid content of the barley shochu distillation residue) has been described to have a suppressive action against orotic acid-induced liver injury and D-galactosamine-induced liver injury. However, the Non-Patent Document 1 to Non-Patent Document 4 do not suggest whether the liquid content of the barley shochu distillation residual liquid has a blood pressure lowering effect. Moreover, there is no suggestion as to whether or not each fraction described in Patent Documents 1 to 4 has the blood pressure lowering action. That is, no example has been known so far in which a fraction having a blood pressure lowering action is separated from the liquid content of the barley shochu distillation residue. And the above-mentioned action which each fraction indicated in patent documents 1 thru / or patent documents 4 has clearly different from a blood pressure lowering action, and this blood pressure lowering action is based on the action given in these patent documents. It cannot be easily predicted.

In view of the above-described prior art, the present inventors aim to obtain a substance exhibiting an excellent blood pressure lowering effect by using a barley shochu distillation residue produced as a by-product when producing barley shochu. It was examined through experiments. As a result, the barley shochu distillation residue is solid-liquid separated to obtain a liquid component, and the liquid component is subjected to an adsorption treatment using a synthetic adsorbent to obtain a synthetic adsorbent adsorbed fraction. The desorption fraction fractionated by eluting the fraction with alkali or ethanol exhibited an excellent blood pressure lowering action in the blood pressure lowering action test using rats. This discovery of the barley shochu distillation residue is an unprecedented new fact. The present invention is based on this discovery, and an object of the present invention is to provide a composition exhibiting a blood pressure lowering action taken from a barley shochu distillation residue.
The barley shochu distillation residue is solid-liquid separated to obtain a liquid, and the liquid is subjected to an adsorption treatment using a synthetic adsorbent to obtain a synthetic adsorbent adsorbed fraction. The synthetic adsorbent adsorbed fraction A desorbed fraction fractionated by elution with an alkali or ethanol is disclosed in Patent Document 2. However, the pharmacological action of the desorption fraction disclosed in Patent Document 2 is an action that suppresses the onset of orotic acid-induced fatty liver and / or D-galactosamine-induced hepatitis, and lowers blood pressure using rats. It is clearly different from the blood pressure lowering effect exhibited in the action test, and this blood pressure lowering action can be easily predicted from the action of suppressing the onset of the orotic acid-induced fatty liver and / or D-galactosamine-induced hepatitis. It is not possible. Thus, this invention provides a useful and novel use about the fraction fractionated from the barley shochu distillation residue.

  The present invention has been completed based on the knowledge obtained through experiments, as described in paragraph 0005 above. The experiments conducted by the present inventors will be described in detail below. That is, the present inventors conducted the following experiment in view of the pharmacological action of barley shochu distillation residual liquid, considering that barley shochu distillation residual liquid may have other useful uses.

First, barley shochu was produced for the purpose of the following experiment. Barley (70% refined) was used as a raw material.
[Manufacture of firewood]
40% barley water absorbed, steamed for 40 minutes, allowed to cool to 40 ° C, inoculated with 1 kg of seed meal (birch) per ton of barley, 38 ° C, RH95% for 24 hours, 32 ° C, RH92% The barley koji was manufactured by holding for 20 hours.
[Manufacture of steamed barley]
Steamed barley was produced by absorbing 40% by weight of barley, steaming for 40 minutes, and allowing to cool to 40 ° C.

[Production of barley shochu and barley shochu distillation residue]
In the first preparation, barley koji (3 tons as barley) produced by the above method was added with 3.6 kiloliters of water and 1 kg (wet weight) of cultured cells of shochu yeast as yeast to obtain primary mash. The first moromi was subjected to fermentation for 5 days (first stage fermentation). Next, in the secondary charging, 11.4 kiloliters of water and steamed barley (7 tons as barley) produced by the method described above were added to the primary mash after the first stage fermentation, and fermentation for 11 days (2 (Stage fermentation). The fermentation temperature was set to 25 ° C. for both the primary charge and the secondary charge. The secondary mash after the second stage fermentation was subjected to simple distillation by a conventional method to obtain 10 kiloliters of barley shochu and 15 kiloliters of barley shochu distillation residue. The obtained barley shochu distillation residue was used in the following experiment.

  That is, the barley shochu distillation residue is centrifuged at 8000 rpm for 10 minutes to obtain a liquid content (a) of the barley shochu distillation residue, and the resulting liquid content (a) is freeze-dried. The lyophilized product (a ′) of the part (a) was obtained, and the lyophilized product (a ′) was used in the following experiment.

[Measurement of blood pressure lowering effect]
The blood pressure lowering action of the lyophilized product (a ′) of the liquid content (a) of the barley shochu distillation residual liquid was measured by the same blood pressure lowering action evaluation test described in Test Example 1 described later.

  As a result, the following facts were found from the measurement results of the blood pressure lowering effect of the lyophilized product (a ′) of the liquid (a) of the barley shochu distillation residue. That is, the lyophilized product (a ′) of the liquid (a) of the barley shochu distillation residue may not exhibit a substantial blood pressure lowering action in the same blood pressure lowering action evaluation test as described in Test Example 1. found.

  Therefore, the inventors of the present invention described in Patent Document 2 “a barley shochu distillation residue obtained as a by-product in the production of shochu using barley as a raw material is subjected to solid-liquid separation to obtain a liquid component. The desorption fraction obtained by subjecting the adsorption treatment to be used to obtain an adsorbed fraction and eluting the adsorbed fraction with an alkali or ethanol ”is the aforementioned orotic acid-induced fatty liver and / or D-galactosamine. In order to clarify whether or not it has a blood pressure lowering action as another pharmacological action different from the action to suppress the onset of induced hepatitis, the following experiment was conducted.

[Acquisition of synthetic adsorbent adsorbed fraction from barley shochu distillation residue liquid]
For the purpose of subjecting to the following experiment, a synthetic adsorbent adsorbed fraction was fractionated from the liquid content of the barley shochu distillation residual liquid according to the following method.
That is, the barley shochu distillation residue is centrifuged at 8000 rpm for 10 minutes to obtain a liquid content (a) of the barley shochu distillation residue, and the obtained liquid content (a) 25 L and deionized water 10 L in this order. After contacting with a column (resin capacity: 10 L) packed with Organo synthetic adsorbent Amberlite XAD-16, the column was charged with 10 L of 1 (wt / vol)% sodium hydroxide solution and 10 L of deionized water. By contacting in order, 20 L of an eluate (b) containing an adsorbed fraction exhibiting adsorptivity to the column was obtained. Further, 20 L of the eluate (b) was brought into contact with a column (resin capacity 10 L) packed with a strongly acidic cation exchange resin IR-120B manufactured by Organo Corporation, and then subjected to freeze-drying, whereby the adsorption fraction from which sodium ions had been removed Lyophilized product (b ′) of 270 g was obtained, and the lyophilized product (b ′) was used in the following experiment.

[Measurement of blood pressure lowering effect]
The blood pressure lowering action of the freeze-dried product (b ′) of the adsorbed fraction (b) was measured by the same blood pressure lowering action evaluation test described in Test Example 1 described later.

  The following facts were found from the measurement results of the blood pressure lowering effect of the freeze-dried product (b ′) of the adsorbed fraction (b) obtained from the barley shochu distillation residue. That is, it was found that the lyophilized product (b ′) of the adsorbed fraction has an excellent and powerful blood pressure lowering effect.

Therefore, the component composition of the synthetic adsorbent adsorbed fraction of the liquid portion of the barley shochu distillation residual liquid that was found to exhibit a blood pressure lowering action was measured by the following method.
[Analysis of component composition of synthetic adsorbent adsorbed fraction]
The component composition of the synthetic adsorbent adsorbed fraction was analyzed. That is, the method of “manufacture of barley shochu and barley shochu distillation residue” described in the paragraph No. 0008 was performed a plurality of times to prepare a plurality of barley shochu distillation residue with different lots. Each barley shochu distillation residue is centrifuged in the same manner as employed in “Acquisition of synthetic adsorbent-adsorbed fraction from barley shochu distillation residue liquid” described in paragraph 0013 above, and barley shochu distillation residue is obtained. The liquid content of the liquid was obtained, and the obtained liquid content of 25 L and deionized water of 10 L were brought into contact with a column (resin capacity: 10 L) filled with the synthetic adsorbent Amberlite XAD-16 manufactured by Organo in this order. By eluting the adsorbed synthetic adsorbent adsorbed fraction, an analytical sample comprising the synthetic adsorbent adsorbed fraction was obtained. In this way, a plurality of types of analytical samples were produced. The protein, sugar composition, polyphenol and organic acid composition of the analytical sample comprising each synthetic adsorbent adsorbed fraction were measured. Crude protein content was measured by Kjeldahl method, sugar composition was measured by HPLC method using hydrochloric acid hydrolysis, polyphenol was measured by Folin-Ciocalteu method, and organic acid composition was measured by HPLC method. Table 1 shows the analysis results of the component composition (based on dry weight) of the synthetic adsorbent adsorbed fraction. As is apparent from the results shown in Table 1, the synthetic adsorbent adsorbed fraction was composed of 40 to 60 wt% crude protein, 7 to 12 wt% polyphenol, 5 to 10 wt% polysaccharide (sugar composition: glucose 0 to 2% by weight, 3-5% by weight xylose, and 2-5% by weight arabinose), 4-10% by weight organic acid (1-3% by weight malic acid, 2-4% by weight citric acid, 0-1% by weight succinic acid) %, Lactic acid 0 to 6% by weight, and acetic acid 0 to 1% by weight) and free sugars 0 to 2% by weight (maltose 0 to 1% by weight, xylose 0 to 1% by weight, arabinose 0 to 1% by weight, and glucose 0 To 1% by weight).
The method for preparing the analytical sample was performed using a synthetic adsorbent other than the synthetic adsorbent Amberlite XAD-16, and each of the liquid components of the barley shochu distillation residue was used as a synthetic adsorbent adsorption image. When an analysis sample consisting of minutes was obtained and the analysis sample thus obtained was analyzed in the same manner as described above, a result substantially equivalent to that shown in Table 1 was obtained.

[Measurement of molecular weight distribution]
Furthermore, in order to clarify the molecular weight of the synthetic adsorbent adsorption fraction of the liquid portion of the barley shochu distillation residue, the molecular weight distribution of the synthetic adsorbent adsorption fraction was measured.
That is, 0.05 W obtained by dissolving separately a molecular weight standard product consisting of Shodex standard P-82 (molecular weight 1300 to 1660,000) and maltotriose (molecular weight 504) manufactured by Showa Denko KK in 0.1 mol / L sodium nitrate solution. A standard solution of / V% concentration was obtained, and the standard solution was injected into a high performance liquid chromatograph to prepare a calibration curve. Next, 0.02 g of each lyophilized product obtained in Experiment 1 to Experiment 7 was prepared, 10 ml of 0.1 mol / L sodium nitrate solution was added thereto, and allowed to stand overnight at room temperature, and then a membrane filter having a pore size of 0.45 μm. Filtration was performed to obtain a filtrate, the filtrate was injected into a high performance liquid chromatograph, and a molecular weight distribution was determined using a 480 Data Station GPC program manufactured by System Instruments Co., Ltd. For high performance liquid chromatographic analysis, Shodex GPC SYSTEM-21 manufactured by Showa Denko Co., Ltd. is used, and a differential refractometer RI-71S manufactured by Showa Denko Co., Ltd. is used as a detector, and the column is TSKgel GMPWXL (φ7.8 mm manufactured by Tosoh Corporation 2 × 300 mm) were connected and used. The column temperature was 40 ° C., a 0.1 mol / L sodium nitrate solution was used for the mobile phase, the flow rate was 1.0 ml / min, and the sample injection volume was 100 μl.

  As a result of measuring the molecular weight distribution of the synthetic adsorbent adsorbed fraction by the above method, it was found that the molecular weight of the synthetic adsorbent adsorbed fraction was substantially 3,000 or less.

  From the above, from the synthetic adsorbent adsorption fraction obtained by subjecting the liquid content of the barley shochu distillation residue in the present invention, that is, the liquid obtained by solid-liquid separation of the barley shochu distillation residue, to the adsorbent treatment It has been found that the composition exhibiting blood pressure lowering action has a high crude protein content, further contains polyphenol, polysaccharide, organic acid, and free saccharide, and its molecular weight is substantially 3,000 or less.

  By the way, the said nonpatent literature 4 describes that the fiber component which consists of barley groove strip contained in the barley shochu distillation residual liquid exhibits a blood pressure lowering effect | action. However, the synthetic adsorbent-adsorbed fraction is obtained by subjecting the barley shochu distillation residue to a solid-liquid separation treatment, and removing the solid content containing the fiber component consisting of barley groove strips for the synthetic adsorbent treatment. Since it is obtained by attaching, it does not contain any fiber component consisting of barley groove. Therefore, the outstanding powerful blood pressure lowering action exhibited by the synthetic adsorbent adsorbed fraction is not derived from the fiber component composed of barley groove.

  Moreover, although the barley shochu distillation residual liquid contains about 0.2% of γ-aminobutyric acid having a blood pressure lowering action, it did not show a substantial blood pressure lowering action in the blood pressure lowering action evaluation test. On the other hand, the synthetic adsorbent adsorbed fraction was found to contain about 0.1% of γ-aminobutyric acid as a result of amino acid analysis using an amino acid analyzer. Therefore, it is clear that the excellent powerful blood pressure lowering action exhibited by the synthetic adsorbent-adsorbed fraction is not derived from γ-aminobutyric acid contained in the synthetic adsorbent-adsorbed fraction.

  Furthermore, the synthetic adsorbent adsorbed fraction contains 7 to 12% by weight of polyphenol in terms of (+)-catechin. On the other hand, as polyphenols contained in barley, proanthocyanidins such as procyanidin B3, procyanidin T4, prodelphinidin B3, prodelphinidin T1, prodelphinidin T2, prodelphinidin T3, and catechins are known. Although there is no knowledge that the proanthocyanidins and catechins isolated from barley exhibit blood pressure lowering effects, proanthocyanidins extracted from grape seeds or catechins extracted from tea leaves exhibit blood pressure lowering effects. It is known. For these reasons, there may be proanthocyanidins and / or catechins that are known to exhibit the above-described hypotensive action among the polyphenols contained in the composition exhibiting the hypotensive action of the present invention. it was thought.

  Therefore, the lyophilized product (b ′) obtained by the method described in paragraph 0013 was subjected to HPLC analysis as a composition exhibiting blood pressure lowering action of the present invention. The HPLC analysis was performed by UV-Vis detection method (280 nm), the column was C18-5B manufactured by Showa Denko KK, the column temperature was 34 ° C., and the eluent was water: acetic acid: methanol = 1: 2: 7 (volume ratio) was used, and the gradient conditions were added to water so that the eluent was 20% final in 60 minutes, and this composition was maintained for 30 minutes thereafter. At that time, the flow rate was 1.0 ml / min, and the sample solution injection amount was 20 μl. Further, the polyphenol contained in the barley described above, procyanidin B3, procyanidin T4, prodelphinidin B3, prodelphinidin T1, prodelphinidin T2, prodelphinidin T3 and catechins, and the blood pressure lowering action of the present invention. The HPLC peak patterns of the lyophilized product (b ′), which is the presenting composition, were compared. As a result, it was found that there was no coincident peak between the two. From this, it was found that the polyphenol contained in the composition exhibiting blood pressure lowering effect of the present invention is completely different from the above-mentioned polyphenol contained in barley.

As is clear from the above description, the composition exhibiting an excellent blood pressure lowering effect of the present invention is clearly different from any of the substances described in the above-mentioned patent documents and non-patent documents. Turned out to be.
The present invention has been completed based on the facts found through the above experiments.

  According to the present invention, barley shochu distillation residue (barley shochu distillation residue obtained as a by-product in the production of shochu using barley as a raw material) is a relatively simple method, that is, the barley shochu distillation residue is solid-liquid separated. An adsorbed fraction having a remarkable blood pressure lowering action can be obtained by subjecting the obtained liquid to an adsorption separation process using a synthetic adsorbent. This adsorbed fraction can also be used for food or medicine.

  The present invention achieves the above object and provides a composition having a remarkable blood pressure lowering action. That is, an adsorption image obtained by solid-liquid separation of a barley shochu distillation residue obtained as a by-product in the production of shochu using barley as a raw material, and subjecting the liquid to an adsorption separation process using a synthetic adsorbent A composition having a significant blood pressure lowering action is provided.

Although the preferable aspect of this invention is described below, this invention is not limited to these.
The composition exhibiting a significant blood pressure lowering effect of the present invention is produced as follows. That is, the method for producing the composition exhibiting a blood pressure lowering action is a first step of obtaining a liquid component by solid-liquid separation of a barley shochu distillation residue obtained as a by-product in the production of barley shochu using barley, A second step of obtaining a synthetic adsorbent adsorbed fraction by subjecting it to an adsorption treatment using a synthetic adsorbent, and a desorbing fraction obtained by eluting the synthetic adsorbent adsorbed fraction using alkali or ethanol; It consists of 3 steps.
Below, the barley shochu distillation residual liquid and each process which are by-produced in manufacture of the shochu using barley as a raw material used when manufacturing the composition which exhibits the blood pressure lowering effect | action of this invention are explained in full detail.

  The barley shochu distillation residue used in the present invention typically comprises barley koji and steamed barley with a yield of 60 to 70% of refined barley, and the starch contained in the resulting barley koji and steamed barley. Saccharified with the barley koji, subjected to alcohol fermentation with yeast to obtain shochu-ripened mash, and the obtained shochu-ripened mash is distilled using a single distillation apparatus such as vacuum distillation or atmospheric distillation Means a by-product as a distillation residue, that is, a distillation residue of barley shochu.

  In the present invention, when the barley shochu distillation residue is obtained, the barley koji used for the production of barley shochu may be produced under the koji-making conditions used in normal barley shochu production. In particular, Aspergillus kawachii used in the production of barley shochu is preferred. Alternatively, strains belonging to the genus Aspergillus such as Aspergillus awamori used in awamori production and Aspergillus oryzae used in sake production and the like can also be used. Moreover, the yeast used for manufacture of barley shochu can use various yeast for shochu brewing generally used in shochu manufacture.

  In the present invention, the first step of obtaining a liquid component by solid-liquid separation of the barley shochu distillation residue obtained in the distillation step in the production of barley shochu is a raw material barley or barley koji-derived from the barley shochu distillation residue. This is performed for the purpose of removing a SS component such as a water-insoluble fermentation residue and obtaining a clarified liquid. The solid-liquid separation in the first step can be performed by a solid-liquid separation method of a screw press method or a roller press method. In addition to this, pre-separation is performed using a filtration and compression type solid-liquid separator, and then this solid-liquid separation treatment is performed by a method using a centrifugal separator, a diatomaceous earth filtration device, a ceramic filtration device, or a filtration press. May be.

  The second step of obtaining the synthetic adsorbent adsorbed fraction by subjecting the liquid obtained in the first step to an adsorption treatment using a synthetic adsorbent is a component that exhibits a blood pressure lowering action contained in the liquid. The purpose is to perform adsorbing fractionation using the synthetic adsorbent. As the synthetic adsorbent used in the second step, an aromatic, aromatic modified or methacrylic synthetic adsorbent can be used. Specific examples of such a synthetic adsorbent include Amberlite XAD-16 manufactured by Organo Corporation, Sepabead SP850 manufactured by Mitsubishi Chemical Corporation, and Diaion HP20 manufactured by Mitsubishi Chemical Corporation.

  In the third step, a fraction exhibiting a blood pressure lowering effect is obtained by eluting the synthetic adsorbent adsorbed fraction obtained in the second step with ethanol or alkali. In particular, when elution is performed using an alkali, preferred specific examples of the alkali include sodium hydroxide and potassium hydroxide. Since the synthetic adsorbent adsorbed fraction eluted from the synthetic adsorbent using such alkali contains cations such as sodium ions and potassium ions, it can be further subjected to ion exchange treatment. Such ion exchange treatment can be performed using a cation exchange resin or the like. Specific examples suitable as the cation exchange resin include the strongly acidic cation exchange resin IR120 and the weak acid cation exchange resins IRC50 and IRC76 manufactured by Organo, and the strong acid cation exchange resin Diaion SK1B manufactured by Mitsubishi Chemical Corporation. SK104, PK208, weakly acidic cation exchange resins WK10, WK40 and the like can be mentioned. In addition, the synthetic adsorbent adsorbed fraction eluted from the synthetic adsorbent using the alkali can be subjected to neutralization treatment using inorganic acid or organic acid such as hydrochloric acid, acetic acid, citric acid, etc. The synthetic adsorbent adsorbed fraction after the sum treatment can be subjected to a desalting treatment using the cation exchange resin.

  The adsorbed fraction which is the composition of the present invention thus obtained is in a liquid state as it is or a dry powder obtained by subjecting it to freeze-drying or the like, and has a remarkable blood pressure lowering effect. Can be used as

  EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples.

Barley shochu was produced for the purpose of providing the following examples. Barley (70% refined) was used as a raw material.
[Manufacture of firewood]
40% (w / w) of barley was absorbed, steamed for 40 minutes, allowed to cool to 40 ° C, inoculated with 1 kg of seed meal (birch) per ton of barley, 38 ° C, RH 95% for 24 hours, 32 Barley koji was produced by maintaining at ℃ and RH 92% for 20 hours.
[Manufacture of steamed barley]
Barley was absorbed by 40% (w / w), steamed for 40 minutes, and then allowed to cool to 40 ° C. to produce steamed barley.

[Production of barley shochu and barley shochu distillation residue]
In the first preparation, barley koji (3 tons as barley) produced by the above method was added with 3.6 kiloliters of water and 1 kg (wet weight) of cultured cells of shochu yeast as yeast to obtain primary mash. The first moromi was subjected to fermentation for 5 days (first stage fermentation). Next, in the secondary charge, 11.4 kiloliters of water and steamed barley (7 tons as barley) produced by the method described above were added to the primary mash after the first stage fermentation for 11 days (two stages). (Fermentation of the eyes). The fermentation temperature was set to 25 ° C. for both the primary charge and the secondary charge. The secondary mash after the second stage fermentation was subjected to simple distillation by a conventional method to obtain 10 kiloliters of barley shochu and 15 kiloliters of barley shochu distillation residue. The barley shochu distillation residue was used in the following examples.

  The barley shochu distillation residue obtained in the distillation process of barley shochu production is centrifuged at 8000 rpm for 10 min to obtain a liquid content of the barley shochu distillation residue, and 25 L of the resulting liquid is synthesized by Organo. Contact with a column (resin capacity: 10 L) packed with adsorbent Amberlite XAD-16 to obtain a synthetic adsorbent adsorbed fraction adsorbed on the column, and further deionize on the column adsorbed the synthetic adsorbent adsorbed fraction After removing the eluate obtained by bringing 10 L of water into contact, 10 L of 1 (wt / vol)% sodium hydroxide solution and 10 L of deionized water are brought into contact with the column in this order from the synthetic adsorbent adsorbed fraction. 20 L of the resulting eluate was collected. When 20 L of the eluate was brought into contact with a column (resin capacity: 10 L) packed with a strongly acidic cation exchange resin IR-120B manufactured by Organo Corporation, freeze-dried and 270 g of the resulting freeze-dried product was pulverized. The composition which exhibits was obtained.

Comparative Example 1

  The barley shochu distillation residue obtained in the distillation process of barley shochu production was centrifuged at 8000 rpm for 10 minutes to obtain 10 L of barley shochu distillation residue liquid, and the obtained barley shochu distillation residue liquid was frozen. By subjecting to drying, 765 g of a freeze-dried product was obtained. The lyophilized product was pulverized to obtain a brown composition.

  The freeze-dried powders obtained in Example 1 and Comparative Example 1 were subjected to the following Test Example 1, and their blood pressure lowering effects were evaluated.

Test example 1

  In order to clarify the blood pressure lowering action of the composition of the present invention, the following test was conducted. That is, 24 male SHR rats of 4 weeks old (Japan SLC) were placed in individual stainless gauges, the temperature in the breeding room was adjusted to 23 ± 1 ° C., and the lyophilized product obtained in Example 1 and Comparative Example 1 After freely ingesting a standard chow diet and water containing none at all for 60 days, each group was divided into 3 groups consisting of a control group, a test group and a comparative group as 8 animals. At that time, the 24 rats were distributed so that there was no statistically significant difference in the variance relating to the average body weight of the rats in each group. For the rats in the control group, 10 ml of water per kg body weight was forcibly administered orally, and for the rats in the test group, 10 ml of the prepared aqueous solution A (the prepared aqueous solution A was obtained in Example 1). 10 mg of prepared aqueous solution B per kg body weight (this prepared aqueous solution B is the lyophilized product obtained in Comparative Example 1). Containing 50% by weight of powder) by oral gavage. The heart rate and blood pressure are measured at the time of the forced oral administration, at 3 hours after the forced oral administration and at 6 hours after the forced oral administration. Was performed under anesthesia.

Evaluation 1

Table 2 shows heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean blood pressure (MBP) in the rats of the control group, the test group, and the comparison group. The following facts were found from the results shown in Table 2.
That is, in comparison between 6 hours after gavage and 6 hours after gavage, the values of systolic blood pressure (SBP) and diastolic blood pressure (DBP) in the comparison group did not decrease significantly, whereas in the test group The value of systolic blood pressure (SBP) decreased significantly. The value of systolic blood pressure (SBP) in the test group 6 hours after gavage was significantly lower than the value of systolic blood pressure (SBP) in the control group 6 hours after the oral administration. found. From the above experimental results, the lyophilized product powder obtained in Comparative Example 1 did not exhibit a substantial blood pressure lowering action, whereas the lyophilized product powder obtained in Example 1 had a significant blood pressure lowering. It was found to have an effect.

  From the above results, it was found that the freeze-dried powder of the present invention obtained in Example 1 showed a significant blood pressure lowering effect on SHR rats, that is, spontaneously hypertensive rats. From this, it was revealed that the composition of the present invention has a remarkable blood pressure lowering effect.

  As can be seen from the results described in Test Example 1, the composition of the present invention was found to have an outstanding blood pressure lowering effect.

  Since the composition of the present invention has a remarkable blood pressure lowering action, it can be suitably used not only for pharmaceutical use but also for food use.

Claims (6)

The barley shochu distillation residue obtained as a by-product in the production of shochu using barley as a raw material is subjected to solid-liquid separation to obtain a liquid, and the liquid is subjected to an adsorption treatment using a synthetic adsorbent to obtain a synthetic adsorbent-adsorbed fraction. A composition having a blood pressure lowering action, comprising a desorption fraction obtained by eluting the synthetic adsorbent adsorption fraction with an alkali or ethanol. 2. The composition according to claim 1, wherein the desorption fraction is in the form of a lyophilized powder. The desorbed fraction is 40 to 60% by weight of crude protein, 7 to 12% by weight of polyphenol, 5 to 10% by weight of polysaccharide (sugar composition: 0 to 2% by weight of glucose, 3 to 5% by weight of xylose) Arabinose 2 to 5 wt%), organic acid 4 to 10 wt% (malic acid 1 to 3 wt%, citric acid 2 to 4 wt%, succinic acid 0 to 1 wt%, lactic acid 0 to 6 wt%, and Acetic acid 0 to 1% by weight) and free sugars 0 to 2% by weight (maltose 0 to 1% by weight, xylose 0 to 1% by weight, arabinose 0 to 1% by weight, and glucose 0 to 1% by weight) The composition according to claim 1, having a molecular weight of substantially 3,000 or less. The composition according to claim 1, wherein the synthetic adsorbent is an aromatic synthetic adsorbent or a methacrylic synthetic adsorbent. The composition according to any one of claims 1 to 4, which is used as a medicine. The composition according to any one of claims 1 to 4, which is used as food.