JP2005278728A - Simple follicle liquid sucking device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To enable the recovery of a follicle liquid passed through a syringe needle mounting tube to its inside by letting the liquid run along the inner wall face of a syringe outer cylinder, prevent bubbling in the follicle liquid in the syringe outer cylinder since new follicle liquid is always supplied on the liquid surface of the follicle liquid, and to secure a normal growth by preventing oxidation/modification of the follicle liquid by this method. <P>SOLUTION: In the simple follicle liquid sucking device, the syringe needle mounting tube for attachably and detachably fitting a syringe needle is integrally formed on the distal end face of the syringe outer cylinder, and the rear end face of this syringe needle mounting tube is flush with the inner wall face of the syringe outer cylinder. A stopper is attachably and detachably fitted to the inside of the rear end opening of the syringe outer cylinder. A through hole is formed in this stopper and a catheter needle pierces through this through hole from outside of the syringe outer cylinder and reaches its inside. Decompression of the inside of the syringe outer cylinder can be executed by a decompression means via this catheter needle. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a simple follicular fluid suction device, and is mainly used when preparing a mature medium for immature ova in mammals.

When in vitro maturation of immature eggs is performed, a large amount of follicular fluid is required to prepare the maturation medium.
For this reason, conventionally, a method has been employed in which follicular fluid is collected into a centrifuge tube by puncturing and sucking an injection needle into the follicle.

Japanese Patent Laid-Open No. 11-206792 Maturation medium NCSU37 `` Petters RM, Wells KD. Culture of pig embryo .J prod Fertil 1993; 48 (suppl): 61-73 ''

  However, in such a conventional follicular fluid suction device, since the suction tube from the injection needle protrudes into the centrifuge tube, the liquid surface of the follicular fluid accumulated in the centrifuge tube reaches this suction tube. When this occurs, bubbling occurs in the follicular fluid, and as a result, the follicular fluid is oxidized and denatured, which has the disadvantage of easily affecting the development.

  An object of the present invention is to eliminate such inconvenience.

  In order to achieve the above object, a simple follicular fluid suction device according to the present invention comprises a syringe outer cylinder, a plug, a catalan needle, an injection needle, and a decompression means, and the injection needle is provided on a distal end surface of the syringe outer cylinder. An injection needle attachment tube for removably fitting the syringe needle, and a rear end surface of the injection needle attachment tube is flush with an inner wall surface of the syringe outer tube, and the syringe outer tube The plug body is detachably fitted in the rear end opening, and a through hole is formed in the plug body, and the catalan needle penetrates from the outside of the syringe outer cylinder into the through hole. The inside of the syringe outer cylinder can be depressurized by the depressurizing means via the cattellan needle.

  The through hole of the stopper can be formed at a position opposite to the injection needle attachment tube with respect to the center of the syringe outer cylinder.

  Note that the tip of the cattellan needle may be directed toward the inner wall surface of the syringe outer cylinder and reach the vicinity thereof.

  Further, a syringe filtration filter may be interposed between the cattellan needle and the pressure reducing means.

  In addition, a syringe inner cylinder can be fitted in place of the stopper.

  Further, an overhang portion can be provided on the root portion of the syringe outer cylinder.

A simple follicular fluid suction device according to the present invention comprises a syringe outer cylinder, a plug, a cattellan needle, an injection needle, and a decompression means, and the injection needle is detachably fitted to the distal end surface of the syringe outer cylinder. And the rear end surface of the injection needle mounting tube are flush with the inner wall surface of the syringe outer tube, and the rear end opening of the syringe outer tube A stopper is removably fitted inside, and a hole is formed in the stopper, and the catalan needle penetrates from the outside of the syringe outer cylinder to reach the inside. And because the inside of the syringe outer cylinder can be depressurized by the depressurizing means through the cattellan needle,
Reducing the inside of the syringe outer cylinder with the injection needle punctured into the follicle, thereby collecting the follicular fluid that has passed through the syringe needle attachment tube along the inner wall surface of the syringe outer cylinder. Can do.

  Therefore, if this simple follicular fluid suction device is used, new follicular fluid is always supplied to the liquid surface of the accumulated follicular fluid in the syringe outer tube, so that the follicular fluid in the syringe outer tube is supplied to the follicular fluid in the syringe outer tube. Bubbling does not occur, and as a result, oxidation and degeneration of the follicular fluid can be prevented and normal generation can be ensured.

  In addition, since the follicular fluid is directly collected in the syringe outer cylinder without interposing any other member via the injection needle mounting tube, the loss of follicular fluid can be minimized as compared with the conventional case. As well as the risk of contamination.

  Further, since the injection needle is located at the distal end surface of the syringe outer cylinder, the operator collects follicular fluid from the ovary while confirming the suction state by working while holding the syringe outer cylinder. As a result, it is possible to prevent erroneous suction and contamination other than the suction device.

  In addition, since the injection needle, the syringe outer tube, and the cattellan needle are arranged in a substantially straight line, it is difficult to apply a burden to the suction of the follicular fluid, and it is easy to find a failure.

  In addition, since the stopper is installed at the rear end of the syringe outer cylinder, the center of gravity of the simple follicular fluid suction device is located at the rear end, so that the suction device itself is stabilized and the follicular fluid is It is easy to collect in the part.

  In addition, if the through hole of the stopper is formed at a position opposite to the injection needle mounting tube with respect to the center of the syringe outer cylinder, the tip of the catalan needle is transmitted through the inner wall surface of the syringe outer cylinder. The follicular fluid is not accidentally aspirated because it does not face the fluid.

  In addition, if the tip of the catalan needle is directed toward the inner wall surface of the syringe outer cylinder and reaches the vicinity thereof, the tip of the catalan needle is disposed at a position farthest from the liquid surface of the follicular fluid. It is even less likely that the cattellan needle will accidentally aspirate follicular fluid.

  If a syringe filtration filter is interposed between the cattellan needle and the decompression means, contamination of the decompression means can be prevented even if the upstream side of the syringe filtration filter is contaminated.

  If the syringe inner cylinder can be fitted in place of the stopper, the syringe inner cylinder can be dispensed as it is after the follicular fluid is collected. In this case, since it is a syringe, it can be carried out while checking the scale, so that it can be dispensed accurately and efficiently.

  Moreover, since the overhang | projection part is provided in the root part of the said syringe outer cylinder, there is no danger of rotating when it puts on a desk, A follicular fluid collection | recovery operation | work is easy.

  In the present invention, an injection needle attachment tube for detachably fitting an injection needle is integrally formed on the distal end surface of the syringe outer cylinder, and the rear end surface of the injection needle attachment tube is an inner wall surface of the syringe outer cylinder The plug body is detachably fitted in the rear end opening of the syringe outer cylinder, and a through hole is formed in the plug body. The main feature is that the cattellan needle penetrates from the outside of the syringe outer cylinder to reach the inside thereof, and the inside of the syringe outer cylinder can be decompressed by the decompression means via the catalan needle. It is easy to apply when preparing a mature medium for immature ova in mammals.

  Examples of the present invention will be described below.

  FIG. 1 is a partial sectional front view of a simple follicular fluid suction device according to the present invention, FIG. 2 is a sectional view taken along the line II-II in FIG. 1, and FIG.

  In FIG.1 and FIG.2, A is a simple follicular fluid suction device, The syringe (it is disposable with a simple structure) which exists conventionally is used as the principal part.

  Reference numeral 10 denotes a syringe outer cylinder, which is formed of a transparent plastic. The syringe outer cylinder 10 has a bottomed cylindrical shape and has a rear end opening 11. Reference numeral 12 denotes an overhang portion for preventing rotation, which is integrally formed with the rear end portion of the syringe outer cylinder 10.

  Reference numeral 13 denotes an injection needle mounting tube which is integrally formed on the outer peripheral side of the distal end surface of the syringe outer cylinder 10. This injection needle attachment tube 13 is for detachably fitting the injection needle 20. The rear end surface of the injection needle attachment tube 13 is flush with the inner wall surface of the syringe outer cylinder 10.

  Next, reference numeral 30 denotes a silicon stopper, which is detachably fitted in the rear end opening 11 of the syringe outer cylinder 10. This silicon plug 30 corresponds to the “plug” of the present invention. Reference numeral 31 denotes a through hole, which is formed at a position slightly shifted from the center O of the silicon plug 30. The through holes 31 gradually approach the outer peripheral side toward the inside. As shown in FIG. 2, the silicone stopper 30 is attached to the syringe outer cylinder 10 so that the through hole 31 is located on the opposite side of the injection needle attachment tube 13 with respect to the center O of the silicone stopper 30. It is fitted.

  Reference numeral 50 denotes a catalan needle which penetrates the through hole 31 of the silicon plug 30 in an airtight state. The catalan needle 50 is directed toward the inner wall surface of the syringe outer cylinder 10 and reaches the vicinity thereof.

  Reference numeral 60 denotes a suction device (corresponding to the “decompression unit” of the present invention), which is connected to the rear end of the catalan needle 50 through a syringe filtration filter 70. Through the suction device 60, the inside of the syringe outer cylinder 10 can be depressurized. Further, the syringe filtration filter 70 is for preventing contamination in the downstream portion.

  A method of using the simple follicular fluid suction device A configured as described above will be described with reference to FIG.

  First, the suction device 60 is turned on to decompress the inside of the syringe outer cylinder 10. The operator grips the syringe outer cylinder 10 so that the cattellan needle 50 faces upward, that is, with the injection needle attachment tube 13 facing down. Then, the injection needle 20 is punctured into the follicle B and the follicular fluid E is sucked. When the follicular fluid E is stored to some extent in the syringe outer cylinder 10, the suction device 60 is turned off, the silicon stopper 30 is removed, and instead, the syringe inner cylinder 80 is internally fitted and transferred to another tube 91. Repeat this process until the required amount is collected.

It can be used for in vitro maturation of immature eggs as a device for collecting follicular fluid when preparing a maturation medium.

FIG. 1 is a partial sectional front view of a simple follicular fluid suction device according to the present invention. 2 is a sectional view taken along line II-II in FIG. FIG. 3 is a use state diagram of the simple follicular fluid suction device according to the present invention.

Explanation of symbols

A ... Simple follicular fluid suction device
10… Syringe barrel
11… Rear end opening
12… Overhang
13… Injection needle mounting tube
20… Needle
30… Plug body
50… Catelan needle
51… Through hole
60… Suction device (pressure reduction means)
70… Filtration filter for syringe
80… Syringe inner cylinder

Claims (6)

A syringe outer cylinder, a stopper, a catalan needle, an injection needle, and a decompression means are provided, and an injection needle mounting tube for detachably fitting the injection needle is integrally formed on the distal end surface of the syringe outer cylinder. And the rear end surface of this injection needle mounting tube is flush with the inner wall surface of the syringe outer cylinder, and the stopper is removably fitted in the rear end opening of the syringe outer cylinder, and The plug body has a through hole, and the catanelan needle penetrates from the outside of the syringe outer cylinder to reach the inside of the stopper body. A simple follicular fluid suction device characterized in that the inside of the syringe outer cylinder can be depressurized by means. 2. The simple follicular fluid suction device according to claim 1, wherein the through hole of the stopper is formed at a position opposite to the injection needle attachment tube with respect to the center of the syringe outer cylinder. Suction device. The simple follicular fluid suction device according to claim 1 or 2, wherein the tip of the catellan needle is directed toward the inner wall surface of the syringe outer cylinder and reaches the vicinity thereof. 2. The simple follicular fluid suction device according to claim 1, wherein a syringe filtration filter is interposed between the cattellan needle and the decompression means. 2. The simple follicular fluid suction device according to claim 1, wherein a syringe inner tube can be fitted in place of the stopper. The simple follicular fluid suction device according to claim 1, wherein an overhang portion is provided on a root portion of the syringe outer cylinder.

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