JP2005168331A - Method for identifying vehicle with paint mixed with dna - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for quickly and accurately deducing the vehicle of an assailant caused a traffic accident on the basis of a fine paint trace left in the accident vehicle at the accident site, even there is not the eyewitness of the vehicle accident. <P>SOLUTION: This method for identifying the vehicle is characterized by coating the surface of the vehicle with a paint containing a specific DNA fragment having a preliminarily clarified base sequence and then specifying the vehicle with the base sequence of the DNA gathered from the paint released from the vehicle. The method for identifying the vehicle is characterized by managing to specify a vehicle number, an owner, a dealer, a sold date, a maker, a made date, a kind, and a manufacture factory with the specific DNA fragment having the preliminarily clarified base sequence. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a vehicle identification method using a paint containing a DNA fragment, and more specifically, a paint containing a DNA fragment is used for painting a vehicle, and a part of the painted paint is peeled off and collected. The present invention relates to a vehicle identification method for identifying a vehicle using DNA in the paint as an index.

  With the recent development of the car society, the means of transportation in human life has become convenient, while the frequent occurrence of traffic accidents has become a major problem. Once a traffic accident occurs, victims must be remedied uniquely and promptly. In recent years, however, there are some cases where victims are left unattended and the perpetrators escape.

  In such a case, the owner of the offending vehicle is specified by comparing the vehicle registration number of the offending vehicle with the vehicle registration number registered with the Land Transport Bureau. However, with these methods, it is impossible to identify the accident witness if there is no witness, and even if there is a witness, the witness must be relied on and the witness must be relied on. Since the memory is often ambiguous or the memory is wrong, it is uncertain to identify the car. Therefore, even if there is no witness, the vehicle is determined from the physical evidence (damaged pieces of vehicle, peeling paint, tire marks, etc.) left by the perpetuating vehicle itself. The labor of judicial police officers has become enormous, and there is a need for a simpler vehicle identification method.

  By the way, an identification method using a DNA fragment is known as an identification method using a very small amount of substance. For example, a method of adding DNA to a liquid and marking it and adding it to the DNA, and detecting the DNA using a PCR method (Patent Document 1), a method of using a nucleic acid as a method for marking a valuable valuable (Patent Document 2) A method for identifying different substances using nucleic acids is known (Patent Document 3), but a method for utilizing DNA itself for vehicle identification is not known.

US Pat. No. 5,643,728 JP-A-3-58800 International Publication No. WO 90/14441

  Therefore, the present inventors, etc., in a social mission to quickly and accurately identify the perpetrators of particularly malicious drowning accidents among the frequent traffic accidents, and to contribute to the reduction of traffic accidents, We will make a swift study on how to quickly and accurately determine how to identify the vehicle by adding DNA to the paint to be painted on the vehicle and using a trace amount of paint trace collected from the accident site. This is the issue.

  As a result of intensive investigations by the present inventors in order to solve such problems, the base sequence is known in advance in the paint used for coating the surface of the vehicle, and a DNA fragment having a unique distinguishability is blended, and the accident vehicle The DNA contained in the paint is detected by removing the paint from the surface, or by collecting the trace paint trace left by the accident vehicle at the accident site. By comparing the registered vehicle DNA with the detected DNA, the injurious vehicle is identified, and a method for easily determining its owner has been found, and the present invention has been completed.

  That is, according to the first aspect of the present invention, which is a basic aspect of the present invention, the surface of the vehicle is painted using a paint containing a specific DNA fragment whose base sequence is known in advance, and then peeled off from the vehicle. A vehicle identification method is characterized in that a vehicle is identified using a DNA base sequence collected from paint as an index.

  More specifically, the present invention described in claim 2 is based on the specific DNA fragment whose base sequence is known in advance, and the vehicle number, owner, distributor, date of sale, manufacturer, date of production. The vehicle identification method according to claim 1, wherein management is performed such that a type and a production factory are specified.

  In this case, the number of bases in the base sequence of the DNA fragment can be determined by the number of vehicles to be identified. Therefore, the present invention described in claim 3 is the vehicle identification method according to claim 1 or 2, wherein the number of bases in the base sequence of the DNA fragment is determined by the number of vehicles to be identified.

  According to a more specific aspect of the present invention, the content of the DNA fragment contained in the paint is about 1 ng per liter of the paint. The vehicle according to any one of claims 1 to 3 It is an identification method.

  According to the vehicle identification method provided by the present invention, it is possible to quickly and accurately determine the accident-damaging vehicle. In particular, even if there are no witnesses of the accident, it has the advantage that it can identify the offending vehicle from the trace traces of paint on the vehicle left on the accident site and easily determine its owner . Further, not only the accident vehicle, but also an accident vehicle, a stolen vehicle, a suspicious vehicle whose owner is unknown, and the like can be used to identify the owner.

  Therefore, the vehicle identification method provided by the present invention can quickly and accurately determine the vehicle and its owner, leading to early resolution and eradication of traffic accidents, leaving accidents, etc. Is.

In the method of the present invention, as described above, the base surface of the DNA collected from the paint peeled off from the vehicle is coated with a paint containing a specific DNA fragment whose base sequence is known in advance. The vehicle identification method is characterized in that the vehicle is identified by using as an index.
That is, the vehicle identification method provided by the present invention uses the unique identification of DNA to mix a specific DNA fragment whose base sequence is known in vehicle paint, thereby leaving the vehicle in which the accident occurred. The paint is extracted from the traces of the paint, the DNA contained in the paint is amplified by the PCR method, and the base sequence is determined, so that the DNA in the paint and the government and administrative supervisory authorities (the Land Transport Bureau) are registered in advance. By collating the identity with the previously registered vehicle DNA fragment, the accident vehicle can be identified and the perpetrator can be determined.

More specifically, the vehicle identification method of the present invention can be performed, for example, from the following steps.
Step 1: A step of preparing a DNA-containing paint by blending a specific DNA fragment whose base sequence is known in a paint to be applied to a vehicle.
Step 2: A paint is applied to the vehicle using the paint. In this painting process, it is recorded in a storage medium such as a computer so that the vehicle to be painted and the DNA fragments contained in the paint to be painted can be confirmed and verified. This is a process of managing the vehicle production number, vehicle manufacturer, production factory, production date, etc. from the DNA base sequence in the paint.
Step 3: A step of removing paint from a vehicle that needs to be identified or collecting traces of paint adhering to the accident site.
Step 4: Add or suspend organic solvent to the paint traces peeled off or collected, extract DNA with water, amplify the obtained DNA by PCR method, determine the nucleotide sequence, and identify the characteristics of the DNA Process.
Step 5: A step of comparing the identified DNA with the registered DNA of the vehicle to identify the vehicle, and to specify the owner or the vehicle manufacturer, the production factory, or the production date.

The vehicle identification method provided by the present invention is basically performed through the above steps, but it goes without saying that various modifications are possible as necessary.
Hereinafter, each step will be described in more detail.

  In step 1, the paint to be applied to the vehicle is a paint that is usually used as a vehicle paint. Specifically, the producer of each vehicle can arbitrarily select it. Generally, a vehicle paint is performed by sequentially coating a vehicle body outer plate portion with a white to gray colored base paint, a titanium oxide-coated mica powder-containing base paint, and a clear paint. Therefore, it is preferable to mix the DNA fragments into the clear paint in order to remove the paint, collect traces and reliably sample the DNA in the paint. It should also be chosen to do.

  The DNA fragment used in the method of the present invention is a specific DNA fragment whose base sequence is known. Such a DNA fragment can be prepared using a general DNA synthesizer, for example, a commercially available DNA synthesizer. By the way, DNA is composed of four types of base components, adenine (A), thymine (T), guanine (G), and cytosine (C). When combined, 1000 trillion combinations are possible. Therefore, by using at least 30 base sequences, 1000 trillion vehicles can be identified, which is sufficient for identifying all vehicles in the world. Moreover, since the quantity of DNA can be increased / decreased by adjusting the long chain of a base sequence, the long chain of a base sequence can be selected according to the identification purpose.

  In the method of blending the DNA fragments into the paint, the DNA fragments are suspended in an organic solvent, and the suspension is added to the paint and sufficiently stirred so that the DNA fragments are almost uniform in the paint. In addition, you may add means, such as a heating, as needed. As the organic solvent to be used, any solvent can be used as long as it is used for coating, but it is generally preferable to use toluene.

  The amount of DNA fragments mixed in the paint amplifies the DNA by the PCR method, so at least one molecule of DNA is basically recovered from the peeling paint derived from the car body paint and the paint trace collected from the accident site. Is sufficient for identification. Therefore, if 1 ng (nanogram) of DNA fragment is blended per liter of paint, even if the release paint or trace paint derived from car body paint is about 1 ng, the DNA contained therein is collected and PCR is performed. The base sequence can be identified by amplification by the method. At this concentration, the DNA fragment was soluble in all paint solvents currently used.

  In step 2, the DNA fragment-containing paint prepared in step 1 is applied to the vehicle by a normal painting method. For the details of the painting, the painting means used by various vehicle manufacturers can be adopted.

  Here, the target vehicle is identified by collating the DNA contained in the paint painted on the vehicle with the base sequence of the DNA detected from the paint collected from the accident site. Needless to say, identification of the vehicle producer / factory, production date, production lot, etc. are performed. Therefore, the characteristics of the DNA fragments contained in the paint and the identity of the vehicle to be painted are recorded in a storage medium such as a computer. This is preferably performed by reporting to the government or administrative supervisory authority (land transportation station) in the same manner as vehicle number registration, and vehicle DNA registration is preferably performed simultaneously with vehicle number registration. In addition, when newly repainting the vehicle, the new paint DNA must be reported to the supervisory authority. Vehicles that have been painted with DNA that does not contain DNA or that contain unregistered DNA are not registered as vehicles. Can be treated as a suspicious car.

  The vehicle identification method provided by the present invention is to identify all vehicle owners by blending a specific DNA fragment whose base sequence is known in one vehicle and one vehicle body paint. Can do. However, if the number is too large, the number of types of DNA to be used can be reduced by grouping and identifying by category such as each vehicle manufacturer / production factory, production date, production lot (unit), etc. be able to. In this case, the vehicle owner cannot be immediately and uniquely identified, but can be narrowed down to a certain range, which can greatly contribute to the so-called investigation.

  Next, Step 3 to Step 5 are steps for identifying the vehicle by a vehicle accident or the like and determining the vehicle owner or the like.

  For example, in step 3, the paint is removed from a vehicle that needs to be identified due to a traffic accident or the like, or traces of the paint left at the accident site are collected. Here, the vehicles that need to be identified include accident victim vehicles, accident victims, stolen vehicles, suspicious vehicles with unknown owners, and the like.

  In step 4, the paint removed or collected in step 3 is suspended in an organic solvent, DNA is extracted with water, DNA in the aqueous layer is concentrated and purified by ethanol precipitation, and the resulting DNA is amplified by PCR. It is a process to do.

  As the organic solvent for suspending the collected paint, a solvent that dissolves the paint is good. Specifically, it is preferable to use toluene. The DNA is extracted from the aqueous layer by a two-phase extraction method or by adding a large amount of water and transferring the DNA to the aqueous layer by centrifugation and extracting the DNA from the aqueous layer. The obtained DNA-containing aqueous solution is dried under reduced pressure or freeze-dried, the residue is subjected to the PCR method, the DNA is amplified, the base sequence is determined, and the owner vehicle is identified.

  For the operation of this PCR method, for example, DNA having a common sequence of 25 bases is combined around 30 bases having different sequences. Therefore, the total DNA used is 80 bases in length. The number of PCRs is amplified until the DNA base sequence becomes possible, but the purpose can be achieved in 30 to 50 cycles, but it is generally preferable to perform amplification for about 40 cycles.

  In step 5, the base sequence of the DNA identified in step 4 and the base sequence information of the vehicle DNA registered in the supervisory authority are collated to identify the vehicle and determine its owner. Also, if the DNA registration form is for the purpose of specifying the vehicle manufacturer, its production factory, production date, production lot, etc., the vehicle manufacturer, its production factory or production date, Production lots can be identified, from which vehicle owners can be narrowed down.

  EXAMPLES Next, the present invention will be described with reference to examples, but the present invention is not limited thereto.

Example 1
1 ng of a DNA fragment consisting of 80 bases per liter of paint was mixed with an organic clear paint containing toluene as a main component, mixed and stirred at room temperature, the DNA fragment was dissolved, and this organic solution was fixed by drying. As the DNA fragment of 80 bases, a DNA fragment whose base sequence was previously known was used. Next, 1 ng of the dried fixed material was peeled and collected, and mixed in a 1: 1 mixture of toluene and water for 10 minutes. Thereafter, the aqueous layer and the toluene layer were separated by centrifugation, the DNA in the aqueous layer was concentrated and purified by the ethanol precipitation method, and then the PCR method (40 cycles) was performed. As a result, DNA necessary for determining the base sequence of the DNA was obtained. The base sequence was actually determined, and the same base sequence as the 80-base DNA mixed in the paint could be confirmed.
As the DNA fragment comprising 80 bases, a DNA fragment having 25 bases having a common sequence around 30 bases arbitrarily selected was used.

As described above, the present invention incorporates DNA registered in advance in the paint to be applied to the vehicle, and is based on the exfoliation and paint traces of the paint collected from the vehicle that requires identification of the vehicle due to an accident. This is a method for identifying a vehicle that needs to be identified, such as an accident vehicle, by collecting DNA in the paint, determining its base sequence, and collating it with the registered DNA. Accordingly, the present invention contributes to the early resolution of traffic accidents, the elimination of accidents, and the public benefit.

Claims (5)

  The vehicle surface is painted using a paint containing a specific DNA fragment whose base sequence is known in advance, and the vehicle is identified using the DNA base sequence collected from the paint peeled off from the vehicle as an index. A vehicle identification method characterized by the above.   It is managed so that the vehicle number, owner, distributor, sales date, manufacturer, date of production, type, and production factory are specified by a specific DNA fragment whose base sequence is known in advance. The vehicle identification method according to claim 1.   The vehicle identification method according to claim 1 or 2, wherein the number of bases in the base sequence of the DNA fragment is determined by the number of vehicles to be identified.   The vehicle identification method according to any one of claims 1 to 3, wherein the content of the DNA fragment is about 1 ng per liter of paint. A vehicle identification method comprising the following steps.
Step 1: A step of preparing a DNA-containing paint by blending predetermined DNA into a paint to be applied to a vehicle.
Process 2: The said coating material is painted on a vehicle. Here, it is recorded on a storage medium such as a computer so that the DNA type can be compared with the painted vehicle, and the owner of the vehicle, the vehicle manufacturer, its production factory or production date is immediately determined from the DNA in the paint. Process to manage so that it can be determined.
Step 3: A step of removing paint from a vehicle that needs to be identified or collecting traces of adhering paint.
Step 4: Organic solvent is added to the exfoliation or collected traces, suspended, DNA is extracted with water, the obtained DNA is amplified by PCR, and the nucleotide sequence is determined to identify the characteristics of the DNA. Process.
Step 5: A step of collating the identified DNA with the registered vehicle DNA to identify the vehicle owner or the vehicle manufacturer, its production factory, or the production date.