JP2003139761A - Chemical micro-device, and using method therefor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To restrain a sample from being sucked to remain in a portion contacting with the sample for reaction and/or analysis, to enhance reaction efficiency thereby, and to attain quick and highly sensitive analysis, in a chemical micro-device used for the reaction and the analysis using a very small amount, such as a micro-chip, a micro-array and a micro-reactor. SOLUTION: In this micro-device, a portion contacting with at least one sample is treated with a fluoro-polymer material.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a chemical microdevice such as a microchip, a microreactor or the like used for conducting an analysis or a reaction in a microreactor in a minute amount, and a method of using the same. It is characterized in that it suppresses the sample used for analysis and reaction etc. from contacting and remaining on this chemical microdevice, and enables stable and efficient analysis and reaction with a minute amount. It is a thing.

[0002]

2. Description of the Related Art In recent years, chemical microdevices such as microchips and microreactors have been used in order to improve reaction efficiency and perform rapid and highly sensitive analysis, etc., and efficiently perform a large number of reactions and analyzes. It has been attempted to carry out analysis and reaction with a minute amount by using it.

Here, when an analysis or reaction is performed in a minute amount using the above-described chemical microdevice, the sample is adsorbed to the portion of the chemical microdevice which is in contact with the sample to be reacted or analyzed. However, there are problems that the reaction efficiency is deteriorated and that rapid and highly sensitive analysis cannot be performed.

In recent years, the presence in the atmosphere has
Harmful substances, eg NO_xTo measure the amount of
The use of una chemical microdevices has been studied,
NO _xGases containing chemicals in chemical microdevices
NO through the porous plate_xIs contacted with a collecting solution for collecting
NO in this collection solution_xNO after collecting_xCollect
Reacting the collected solution with the fluorescent reagent solution to produce a fluorescent substance
Then, the fluorescence intensity of this fluorescent substance is detected by a fluorescence detector.
Put it out, NO_xIt has been considered to measure the amount of

However, the amount of the thus in the case of measuring the amount of the NO _x well, the porous plate in the chemical micro devices, collected in the collecting solution was NO _x may remain adsorbed, NO _x There was a problem that it could not be measured accurately.

[0006]

SUMMARY OF THE INVENTION The present invention is intended to solve the above problems in chemical microdevices used for conducting reactions and analyzes in minute amounts such as microchips, microarrays and microreactors. This is an issue.

That is, according to the present invention, when performing an analysis or a reaction in a minute amount, it is possible to prevent the sample from being adsorbed and remaining on the portion of the chemical microdevice which comes into contact with the sample to be reacted or analyzed, It is an object of the present invention to improve the reaction efficiency by allowing the sample to be efficiently used in the reaction and to perform the rapid and highly sensitive analysis.

[0008]

In the chemical microdevice of the present invention, in order to solve the above problems, at least one portion in contact with the sample is treated with a fluoropolymer material. is there.

Then, like the chemical microdevice of the present invention, when the portion contacting with at least one sample is treated with the fluorine-based polymer material, the sample is adsorbed to the portion contacted with this fluorine-based polymer material. Is prevented, the sample is efficiently used for the reaction, the reaction efficiency is improved, and rapid and highly sensitive analysis can be performed.

As the above-mentioned fluorine-based polymer material, it is preferable to use a fluorine ether-based oligomer silane coupling agent such as demnum silane.
In particular, when treated with Demunamushiran is collected in the collecting solution was NO _x is to be inhibited from remaining adsorbed.

[0011]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The chemical microdevice according to the embodiments of the present invention and the method of using the same will be specifically described below with reference to the accompanying drawings, and the chemical microdevices according to the embodiments of the present invention are excellent. Will be clarified by giving a comparative example. However, the chemical microdevice and the method of using the same according to the present invention are not particularly limited to those shown in the following examples, and can be appropriately modified and implemented within the scope of the invention. .

Now, in this embodiment, a chemical microdevice used for measuring the amount of NO ₂ will be described.

In the chemical microdevice of this embodiment,
In addition, as shown in FIG.
50 mm, 26 mm in height, and 1 mm in thickness of the chip substrate 10
Si is deposited on one surface to suppress the influence of scattered light.
After that, chemical etching is performed on one surface of the chip substrate 10 on which Si is deposited.
Microchannels required for reaction of substances
Build NO, NO₂Triethanolamine that collects gas
A first flow path 11 for guiding the collecting solution containing the nitrogen
Along with this, NO ₂Gas
While providing the collecting unit 12 for collecting the above-mentioned collecting solution,
NO₂Reacts with the collection solution that collects the gas to produce a fluorescent substance.
The second channel 13 for guiding the fluorescent reagent solution to be formed is trapped as described above.
Merges with the first flow passage 11 on the downstream side of the collecting portion 12.
And the merging flow path 14 merged in this way.
Form long, NO₂Gas-collecting solution and fluorescence
It is designed so that it can sufficiently react with the reagent solution.

Further, in the chemical microdevice of this embodiment, as shown in FIG.
The porous plate 15 made of glass is arranged on the collecting section 12 on one side of No. 0 so that the NO ₂ gas is brought into contact with the collecting solution in the collecting section 12, and the other parts than the collecting section 12 are arranged. In the above, the chip substrate 10 is covered with the transparent covering plate 16 so that NO ₂ gas does not come into contact therewith.

Here, in the chemical microdevice of this example, a 0.1 wt% solution of demnum silane represented by the following chemical formula 1 diluted with perfluorohexane was dropped on the porous glass plate 15 described above. The porous plate 15 which has been air-dried and treated with demnum silane is used.

[0016]

[Chemical 1]

On the other hand, in the coating plate 16 described above, the first supply port 16a for supplying the above-mentioned trapping solution to the first flow path 11 is provided.
And a second supplying the above fluorescent reagent solution to the second flow path 13.
A supply port 16b and a recovery port 16c for recovering the liquid in the final part of the confluent channel 14 are provided.

When measuring the amount of NO ₂ using the chemical microdevice of this embodiment, NO
₂ A collection solution for collecting gas is supplied to the first flow path 11 through the first supply port 16a provided in the above-mentioned coating plate 16, and in the collection section 12 provided in the middle of the first flow path 11. ,
Contacting the NO ₂ gas introduced through the porous plate 15 above the collecting solution is collected NO ₂ gas.

On the other hand, the fluorescent reagent solution that reacts with the trapping solution that traps NO ₂ gas to produce a fluorescent substance passes through the second supply port 16b provided in the above-mentioned covering plate 16 and the second flow path 13
And guides the fluorescent reagent solution through the second flow path 13 to the merging flow path 14 on the downstream side of the collection unit 12, and collects the NO ₂ gas in the merging flow path 14. Combine the collecting solution and the fluorescent reagent solution.

Then, in this confluence channel 14, NO
_{2 The} collection solution obtained by collecting the gas and the fluorescent reagent solution are reacted with each other to generate a fluorescent substance, and the excitation light is emitted from the excitation light source 20 at an appropriate position of the confluent channel 14 as shown in FIG. The fluorescent substance is irradiated, and the intensity of the fluorescence emitted from this fluorescent substance is measured by the sensor 21 to detect the amount of NO ₂ gas. An LED (manufactured by Nichia Corporation) that emits ultraviolet light having a wavelength of 370 nm is used as the excitation light source 20, and the fluorescence intensity at the center wavelength of 420 nm is obtained.

After the amount of NO ₂ gas is detected as described above, the solution is recovered in the final portion of the confluent channel 14 through the recovery port 16c provided in the covering plate 16. There is.

Next, as described above, the NO ₂ gas collecting section 1 is used.
Porous plate 1 made of glass to be brought into contact with the collecting solution in 2
No. ₂ gas was used as described above, using the chemical microdevices of Examples using the ones treated with demnum silane and the chemical microdevices of Comparative Examples using the ones not treated with demnamsilane. An experiment was conducted to detect the amount of

Here, in an experiment for detecting the amount of NO ₂ gas using each chemical microdevice of the examples and comparative examples, each of the above chemical microdevices was set in the wind tunnel, Supply NO ₂ gas to NO ₂
After changing the concentration from 10 ppb to 200 ppb, N
The supply of O ₂ gas is stopped, the NO ₂ concentration is changed to 10 ppb, the excitation light is emitted from the excitation light source 20 to the fluorescent substance at predetermined time intervals, and the fluorescence intensity emitted from this fluorescent substance is measured by the sensor 21. Measured by

The result of measurement using the chemical microdevice of the example is shown in FIG. 4, and the result of measurement using the chemical microdevice of the comparative example is shown in FIG.

As a result, the chemical microdevice of Comparative Example
If you use ₂Gas is supplied
NO₂Fluorescence intensity increased with increasing concentration
But no₂Gas supply is stopped and NO₂The concentration decreases
The fluorescence intensity does not decrease for a while even when
won. This is the NO collected in the collection solution.₂Is above
It remains in the holes of the glass porous plate 15 and this effect remains.
It is believed that this is because

On the other hand, the chemical microdevice of the embodiment is used.
When using a vise, NO in the wind tunnel₂Gas supplied
Is NO₂Fluorescence intensity increases with increasing concentration
And NO₂Gas supply is stopped and NO₂Concentration
As the fluorescence intensity decreases, it decreases in the wind tunnel.
NO in₂The fluorescence intensity changes depending on the concentration, and NO ₂
The concentration can be measured properly.

In the chemical microdevice of the above embodiment, the trapping solution in the trap 12 and the NO ₂
The glass porous plate 15 that is brought into contact with gas is only treated with demnum silane, but the same treatment can be performed for each flow path and the like, if necessary.

Further, in this embodiment, a chemical microdevice is used for detecting NO ₂ .
It is possible to use not only for gas components but also for detection and reaction of substances in all other states.

[0029]

As described above in detail, in the chemical microdevice according to the present invention, at least one portion which comes into contact with the sample is treated with the fluorine-based polymer material. The sample was prevented from being adsorbed to the contacted portion, the sample was efficiently used in the reaction, the reaction efficiency was improved, and the rapid and highly sensitive analysis became possible.

[Brief description of drawings]

FIG. 1 is a schematic plan view of a chip in a chemical microdevice according to an embodiment of the present invention.

FIG. 2 is a schematic plan view of a chemical microdevice according to the example.

FIG. 3 is a schematic plan view showing a state in which NO ₂ gas is detected using the chemical microdevice according to the example.

FIG. 4 is a diagram showing the results of measurement of change in fluorescence intensity with changes in NO ₂ concentration in the wind tunnel using the chemical microdevice of the same example.

FIG. 5 is a diagram showing the results of measurement of change in fluorescence intensity with changes in NO ₂ concentration in the wind tunnel using a chemical microdevice of a comparative example.

[Explanation of symbols]

10 chip substrate 11 First flow path 12 Collection Department 13 Second channel 14 Confluence channel 15 Porous plate 16 Cover plate 20 Excitation light source 21 sensor

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Masa Shimoma             4-23-7 Omiya, Asahi-ku, Osaka-shi, Osaka             Within Turlite Industry Co., Ltd. F term (reference) 2G042 AA01 BB07 CA01 CB01 DA09                       FA11 FA20 HA02                 2G054 AA01 CA06 CE08 EA03 FA06

Claims (4)

[Claims] 1. A chemical microdevice, characterized in that at least one portion in contact with a sample is treated with a fluoropolymer material. 2. The chemical microdevice according to claim 1, wherein the fluoropolymer material is a fluoroether oligomer silane coupling agent. 3. The chemical microdevice according to claim 1, wherein the fluorine-based polymer material is demnum silane. 4. A method of using a chemical microdevice, wherein the chemical microdevice according to claim 1 is used for measuring NO _x .