JP2003113040A - Antibacterial cosmetic composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an effective and epoch-making antibacterial cosmetic composition friendly to human beings and the environment offering the bactericidal potency of the antibiotic iturin-based peptide for stabilizing cosmetics and making use of the action of the iturin on cell membrane, and having improved percutaneous penetrability of the liposoluble vitamin or the derivative thereof contained in the cosmetics. SOLUTION: This antibacterial cosmetic composition includes the iturin-based peptide as the active ingredient, and the liposoluble vitamin or the derivative thereof.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an antibacterial cosmetic composition containing a highly safe iturin peptide that can be used in cosmetics.

[0002]

2. Description of the Related Art Conventionally, cosmetics come into direct contact with a part of the human body such as hands each time they are used, and further, when the cosmetics container is opened, the cosmetics also come into contact with the external environment via air, etc. There is an inherent risk that so-called bacteria will be mixed into the product and its quality will be significantly reduced.
Therefore, in order to maintain quality, all cosmetics are blended with low toxicity antibacterial and antifungal agents such as phenols and hydroxybenzoic acid esters. In addition to these, mercury may be used for special purposes.

[0003] Of these, for example, phenols have weak antibacterial and fungicidal activity, and therefore, in order to obtain a sufficient effect, it is required to use a higher concentration, but a high concentration of phenols is a coexisting protein. Since it degenerates, it cannot be practically used.

On the other hand, hydroxybenzoic acid esters, so-called parabens, have a bacteriostatic action against fungi, but have no effect on Gram-negative bacteria. In addition, parabens have low solubility in water, for example, when used at low temperatures, they cannot be dissolved in a necessary and sufficient amount, so that a satisfactory effect cannot be expected. Furthermore, with regard to parabens, recently, there is some suspicion that they are so-called endocrine disrupters, and it cannot be said that they are necessarily safe.

[0005] Further, for example, even if parabens are added to cosmetics, microorganisms acquire resistance to them, so that the control effect is rapidly reduced, and finally the efficacy is lost. Problem.

In recent years, there has been a strong demand for cosmetics that are hypoallergenic, and non-colored, fragrance-free and non-alcoholic cosmetics have been developed and dominated the market. However, regarding skin irritation, irritation by preservatives such as parabens is a major factor, and cosmetics that do not use preservatives are also being investigated, but in this case, in order to suppress the propagation of various bacteria, cosmetics should be used. It requires a treatment such as storage at low temperature, which makes it extremely inconvenient to use.

Under these circumstances, iturin peptides used in the present invention are known to have a strong antibacterial action against fungi, and they are used for controlling microbial contamination. How to do is reported. For example, MA Klichi (Mycopathologia 127: 123-127,1994)
Etiulin has a strong antibacterial action against fungi, and at the same time shows high safety against animals,
The control effect of iturin on microbial contamination by molds generated during storage of various grains is measured. As a result, it is reported that the use of iturin at a concentration of 50 to 100 ppm can significantly suppress the generation of mold.

A method in which a technique utilizing the bactericidal activity of iturin is applied to, for example, suppressing the growth of phytopathogenic fungi in the field of agriculture and horticulture is disclosed in JP-A-59-212.
Nos. 416, 61-289005, and 61-289898. Further, Japanese Patent Application Laid-Open No. 7-143897 discloses a method of using iturin for the prevention or treatment of deep-seated mycosis. According to this, various fungal infections are treated by oral administration of iturin or parenteral administration of subcutaneous, intramuscular or intravenous injection.

Regarding the toxicity of iturin, which is a problem in this case, no cytotoxicity was observed at a concentration of 1 to 0.5 mg / mL or less in the cytotoxicity test, and the LD ₅₀ was 2,000 mg in the acute toxicity test. It is shown that the safety of iturin is high with / Kg or more.

[0010]

DISCLOSURE OF THE INVENTION The present inventors have conducted extensive studies on a natural antibacterial substance which has a broad antibacterial spectrum and a strong bactericidal activity, and is extremely safe for humans and can be repeatedly used with peace of mind. It was As a result, iturin-based peptides have excellent antibacterial properties, are easily biodegradable in the environment, and are harmless to humans and animals, and exhibit outstanding properties as cosmetic compositions. The present invention has been completed and the present invention has been completed.

[0011]

The present invention provides an antibacterial cosmetic composition capable of enhancing the antibacterial property of cosmetics and remarkably enhancing the storage stability thereof by incorporating iturin having antibacterial properties into cosmetics. Regarding

That is, the present invention relates to the following items. (1) An antibacterial cosmetic composition containing an iturin peptide as an active ingredient. (2) The antibacterial cosmetic composition according to (1) above, wherein the iturin peptide is a compound represented by the general formula (1).

[0013]

[Chemical 2] (In the formula (1), R represents a linear or branched alkyl group having 3 to 10 carbon chains.)

(3) The antibacterial cosmetic composition according to the above (1) or (2), which contains a fat-soluble vitamin or a derivative thereof. (4) A cosmetic containing the antibacterial cosmetic composition according to any one of (1) to (3). (5) A skin agent containing the antibacterial cosmetic composition according to any one of (1) to (3). (6) The skin preparation according to (5) above, which is a prophylactic or therapeutic agent for acne and atopic dermatitis.

[0015]

BEST MODE FOR CARRYING OUT THE INVENTION The iturin-based peptide used in the present invention is not particularly limited, but is represented by the following formula (1):

[0016]

[Chemical 3]

A compound represented by the formula (1) wherein R represents a linear or branched alkyl group having 3 to 10 carbon chains is preferable. Needless to say, those having a structural change or contraction can be used as long as the effects of the present invention are not impaired.

In the formula, R is a linear or branched alkyl group having 3 to 10 carbon chains, preferably (CH ₃ ) CHC
H ₂ , (CH ₃ ) CHCH ₂ CH ₂ , (CH ₃ ) CHCH ₂ C
H ₂ CH ₂ , CH ₃ CH ₂ CH ₂ , CH ₃ CH ₂ CH (C
_{H 3), CH 3 CH 2} CH 2 CH 2 CH 2, CH 3 CH (C
H ₃ ) CH ₂ CH ₂ , CH ₃ CH ₂ CH ₂ CH ₂ CH ₂ CH _{2 and the} like.

The mechanism of action of iturin is to penetrate into the cell membrane to form pores, in which iturin is known to associate hydrophobically with cell membrane components (R. Ma.
get-Dana et al (Toxicoloogy 87,151-174,1994), C.
Latoud et al (Can. J. Microbiol 36; 384-389, 1990)).

On the other hand, it goes without saying that a wide variety of hydrophobic molecules are also incorporated into cosmetics in accordance with their purposes, and they fulfill the functions required for cosmetics. Iturin can also organically associate with such hydrophobic components,
Inevitably, it has the property of assisting their access to and penetration of skin cells.

Examples of such hydrophobic components include L-ascorbic acid palmitate and L-ascorbic acid palmitate.
Ascorbic acid stearates, vitamins with a long hydrocarbon chain can be mentioned, but these are affected by the enzyme (esterase) existing in the cell membrane and cytoplasm, and after the hydrocarbon chain is modified and digested, Since it is converted into ascorbic acid to exert a physiologically active action, it can be used in cosmetics to supplement human essential vitamins and to bring about useful functions such as its antioxidant action. Furthermore, not only ascorbic acid derivatives but also fat-soluble vitamins, water-soluble vitamins, and stabilized vitamins having a long hydrocarbon chain like the above-mentioned ascorbic acid derivatives can be expected to bring similar effects. .

As described above, according to the present invention, by using a derivative of an organic compound having a physiological action, in addition to the antibacterial action as a stabilizer for cosmetics, it is possible to bring about a beneficial function for humans. Is. Further, not only these derivatives, but also beneficial compounds can be wrapped in a micelle structure formed by iturin hydrophobically associated with each other, and the same effect can be expected.

As described above, iturin cell membrane permeation action and micelle formation result in accelerated skin permeation, and compounds expected to have physiological effects include, for example, vitamin A, provitamin A, vitamin D, Fat-soluble vitamins such as provitamin D, vitamin E, and vitamin K, thiamine, riboflavin, niacin, vitamin B ₆ group, pantothenic acid, biotin, myo-inositol,
Water-soluble vitamins such as choline, holacin, vitamin B ₁₂ , vitamin C and their derivatives, ubiquinone, lipoic acid,
Vitamin-like acting factors such as vitamin F, vitamin B ₁₃ , vitamin B _T , p-aminobenzoic acid, vitamin P and vitamin U and derivatives thereof can be mentioned.

The cosmetic of the present invention contains iturin as an essential component, but in addition, within a range not impairing the effects of the present invention,
Ingredients used in cosmetics, for example, 2nd edition commentary on cosmetic raw material standards, edited by The Church of Japan Official Book, 1984 (Yakuji Nipposha),
Non-standard ingredients for cosmetics, supervised by Ministry of Health, Labor and Welfare Pharmaceutical Affairs Bureau Examination Division,
1993 (Pharmaceutical Daily), supplement of non-standard ingredients for cosmetics, supervision by the Ministry of Health, Labor and Welfare Pharmaceutical Affairs Bureau examination section, 1993 (Pharmaceutical Affairs Daily), approval criteria by makeup type, Ministry of Health, Labor and Welfare Administration Division examination section,
Ingredients described in 1993 (Yakuji Nippo Co., Ltd.), Cosmetic Raw Materials Dictionary, 1991 (Nikko Chemicals), etc. can be added.

For example, oil, higher alcohol, fatty acid,
Examples of materials that can be added, such as ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances and preservatives, are listed below.
Of course, the invention is not limited to these examples.

(1) Example of Oil Ester-based oil phase components: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate ,
Isocetyl isostearate, butyl stearate, butyl myristate, ethyl linoleate,

Isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diisopropyl adipate, isoarachial neopentanoate, tri (capryl. Capric acid) glyceryl, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate. , Cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate, myristin Isotridecyl, isocetyl myristate, isostearyl myristate, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexane Acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol di (capryl, caprate) propylene glycol dicaprylate, neopentyl glycol dicaprate , Neopentyl glycol dioctanoate, glyceryl tricaprylate, triundecyl acrylate Seryl, triisopalmitate, glyceryl triisostearate, glyceryl octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, neodecanoic acid hexyl decyl, neodecanoate octyldodecyl,
Isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, dipropyl carbonate, dialkyl carbonate (C
12-18), triisocetyl citrate, triisoaralkyl citrate, triisooctyl citrate, lauryl lactate,
Myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, adipic acid Diisobutyl, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, 12-stearoyl isosetyl hydroxystearate, 12- Stearyl stearoyl hydroxystearate, 12-stearoyl hydroxystearate Sosuteariru, and the like.

Hydrocarbon-based oil phase components: squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum and the like.

Animal and vegetable oils and hydrogenated oils thereof and naturally occurring waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated lard, horse oil, hydrogenated horse oil, mink oil, orange laffy oil, fish oil, hydrogenated fish oil,
Animal oils such as egg yolk oil and hydrogenated oils thereof, avocado oil, almond oil, olive oil, cacao oil, apricot kernel oil, coconut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, Evening primrose oil, camellia oil, corn oil, rapeseed oil, hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil, sunflower oil, grapes Seed oil, jojoba oil, hardened jojoba oil, macadamia nut oil, medhome oil, vegetable oil such as cottonseed oil, hardened cottonseed oil, coconut oil, hardened coconut oil and its hardened oil, beeswax, high acid value beeswax, lanolin, reduced lanolin, liquid lanolin, Examples thereof include wax such as carnauba wax and montan wax.

Silicone-based oil phase component: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopoly Siloxane, dimethylsiloxane
Methylcetyloxysiloxane copolymer, dimethylsiloxane / methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, dimethiconol, silicone gel, acrylic silicone, trimethylsiloxysilicic acid, silicone RTV rubber, etc. To be

Fluorine-based oil phase components: perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, fluoroalkyl / polyoxyalkylene co-modified organopolysiloxane, and the like.

(2) Examples of higher alcohols Lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2
-Ethylhexanol, hexadecyl alcohol, octyldodecanol and the like can be mentioned.

(3) Examples of fatty acids Caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid,
Examples thereof include stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid, erucic acid and 2-ethylhexanoic acid.

(4) Examples of UV absorbers Paraaminobenzoic acid, amyl paraaminobenzoate, ethyl dihydroxypropyl paraaminobenzoate, glyceryl paraaminobenzoate, ethyl paraaminobenzoate, octyl paraaminobenzoate, octyldimethyl paraaminobenzoate, ethylene salicylate. Glycol, octyl salicylate, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, octyl paramethoxycinnamate, 2-ethylhexyl paramethoxycinnamate,

Diparamethoxycinnamic acid mono-2-ethylhexanoate glyceryl, isopropyl paramethoxycinnamate, paramethoxyhydrocinnamic acid diethanolamine salt, diisopropyl / diisopropylcinnamic acid ester mixture, urocanic acid, ethyl urocanate, hydroxy Methoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, butylmethoxydibenzoylmethane, 2,
4,6-trianilino-p- (carbo-2-ethylhexyl-1-oxy) -1,3,5-triazine, 2-
(2-hydroxy-5-methylphenyl) benzotriazole, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenylacrylate, phenylbenzimidazole sulfate, 3- (4-methylbenzylidene) camphor, Isopropyldibenzoylmethane, 4- (3,4-dimethoxyphenylmethylene)
Examples include 2-ethylhexyl-2,5-dioxo-1-imidazolidinepropionate and the like, and polymer derivatives and silane derivatives thereof.

(5) Examples of powder / pigment Pigments such as red No. 104, red No. 201, yellow No. 4, blue No. 1, black No. 401, yellow No. 4 AL lake, yellow 203
No. BA lake and other rake pigments, nylon powder, silk powder, urethane powder, Teflon (registered trademark)
Polymers such as powder, silicone powder, polymethylmethacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polyethylene powder,
Colored pigments such as yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, ultramarine blue and navy blue, white pigments such as zinc oxide, titanium oxide and cerium oxide, talc, mica,
Body pigments such as sericite, kaolin, and plate-shaped barium sulfate, pearl pigments such as mica titanium, metal salts such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, magnesium silicate, inorganic powders such as silica and alumina, stearin Examples thereof include metal soaps such as aluminum acid salt, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, and zinc undecylenate, bentonite, smectite, and boron nitride. The shape of these powders (spherical,
There is no particular limitation on the particle size (rod shape, needle shape, plate shape, irregular shape, scale shape, spindle shape, etc.).

These powders are conventionally known surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil agent treatment, N-acylation. It may or may not be surface-treated in advance by lysine treatment, polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment and the like.

(6) Examples of surfactants Anionic surfactants: fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkylallyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl. Ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl amide phosphate, alkyloyl alkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, Examples thereof include sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester and the like.

Cationic surfactants: alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium bromide, chloride Examples thereof include benzalkonium, amidopropyldimethylhydroxypropylammonium behenate chloride, diethylaminoethylamide stearate, dimethylaminoethylamide stearate, dimethylaminopropylamide stearate, and lanolin derivative quaternary ammonium salt.

Amphoteric surfactant: carboxybetaine type,
Amidobetaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylic acid salt type, imidazoline derivative type,
Examples thereof include amidoamine type.

Nonionic surfactant: propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE sorbit fatty acid ester, POE glycerin fatty acid ester,
POE alkyl ether, POE fatty acid ester, PO
E hydrogenated castor oil, POE castor oil, POE / POP copolymer, POE / POP alkyl ether, polyether modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid and the like can be mentioned. Natural surfactants: lecithin, saponin, sugar-based surfactants and the like.

(7) Examples of polyhydric alcohol and sugar Ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin,
Diglycerin, polyglycerin, 3-methyl-1,3-
Butanediol, 1,3-butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan. Etc. Further, these chemically modified products and the like can also be used.

(8) Examples of polymer compounds Acrylic acid ester / methacrylic acid ester copolymer (plus size, manufactured by Kyo Kagaku Co.), vinyl acetate / crotonic acid copolymer (resin 28-1310, manufactured by NSC), Vinyl acetate / crotonic acid / vinyl neodecanoate copolymer (28
-2930, manufactured by NSC), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP),
T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (Rubimer, manufactured by BASF), vinylpyrrolidone / vinyl acetate / vinyl propionate copolymer (Rubiscol VAP, manufactured by BASF), vinyl acetate / crotonic acid Copolymer (Rubiset CA, BA
SF company), vinyl acetate / crotonic acid / vinyl pyrrolidone copolymer (Rubiset CAP, BASF company), vinyl pyrrolidone / acrylate copolymer (Rubiflex, BASF company), acrylate / acrylamide copolymer (Ultrahold) Manufactured by BASF),
Vinyl acetate / butyl maleate / isobonyl acrylate copolymer (Advantage, ISP), carboxyvinyl polymer (Carbopol, BFgoodrich), acrylic acid / alkyl methacrylate copolymer (Pamullen, BFgoodrich) Anionic polymer compounds such as acetic acid amphoteric compound of dialkylaminoethyl methacrylate polymer (Yukaformer, manufactured by Mitsubishi Chemical Corporation), octyl acrylamide acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, NSC) Polymer), vinylpyrrolidone / dimethylaminoethylmethacrylate quaternary compound (GAFQUAT, ISP), methylvinylimidazolium chloride / vinylpyrrolidone copolymer (Rubicoat, BASF) Down polymer compound,
Polyvinylpyrrolidone / vinyl acetate copolymer (Rubiscor VA, manufactured by BASF), vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (Copolymer V
C713, manufactured by ISP) and the like. Further, cellulose or a derivative thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharide, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum,
Gum arabic, crystalline cellulose, arabinogalactan,
Naturally-derived polymer compounds such as karaya gum, tragacanth gum, alginic acid, albumin, casein, curdlan, gellan gum and dextran can also be preferably used.

(9) Examples of physiologically active ingredients Examples of the physiologically active ingredients include substances that give some kind of physiological activity to the skin when applied to the skin. For example, whitening ingredients, anti-inflammatory agents, anti-aging agents, UV protection agents, slimming agents, tightening agents, antioxidants, hair growth agents, hair growth agents, moisturizers, blood circulation promoters, antibacterial agents, bactericides, drying agents, Cooling agents, warming agents, vitamins, amino acids, wound healing promoters, stimulants, pain relievers, cell activating agents, enzyme components and the like can be mentioned.

Examples of these suitable compounding ingredients include, for example, ashitaba extract, avocado extract, armature extract, altea extract, arnica extract, aloe extract,
Apricot extract, apricot kernel extract, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract, ages extract, gardenia leaf extract, sardine extract, psyllium extract, laurel extract, barley extract, St. John's wort extract, scabbard extract, Dutch mustard extract, orange extract. , Dried seawater, seaweed extract, hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk,
Chamomile extract,

Carrot extract, sagebrush extract, licorice extract, karkade extract, oyster extract, kiwi extract, kina extract, cucumber extract, guanosine, gardenia extract, kumazasa extract, clara extract,
Walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, black tea extract, yeast extract, burdock extract, rice bran extract, rice germ oil,

Comfrey Extract, Collagen, Cowberry Extract, Saishin Extract, Psychoextract, Saitai Extract, Salvia Extract, Soapless Extract, Sasa Extract,
Hawthorn extract, hawthorn extract, shiitake extract,
Geombu extract, shikon extract, perilla extract, linden extract, spirea extract, peony extract, ginger root extract, birch extract, horse mackerel extract, honeysuckle extract, hawthorn extract, horse mackerel extract, millet extract, mint extract, sage extract, mallow extract. , Senkyu extract, senburi extract, soybean extract, tern extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, touki extract, daffodil extract, tonin extract, spruce extract, dokudami extract, tomato extract, natto extract, carrot extract, Garlic extract, Novara extract, Hibiscus extract, Bakmondou extract, Parsley extract, Honey, Hamamelis extract, Parier Riaekisu, cause extract,
Bisabolol, loquat extract, coltsfoot extract, butterbur extract, butterbur extract, butcher bloom extract, grape extract,

[0048] Propolis, loofah extract, safflower extract, peppermint extract, bodige extract, button extract, hop extract, pine extract, horse chestnut extract, sphagnum extract, sucrose extract, melissa extract,
Peach extract, cornflower extract, eucalyptus extract, Yukinoshita extract, Yuzu extract, Yukuinin extract, Artemisia extract, Lavender extract, Apple extract, Lettuce extract, Lemon extract, Astragalus extract, Rose extract, Rosemary extract, Roman chamomile extract, Royal jelly extract, etc. be able to.

Deoxyribonucleic acid, mucopolysaccharide, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, biopolymer such as hydrolyzed eggshell membrane, amino acid, hydrolyzed peptide, sodium lactate, urea, pyrrolidone Moisturizing ingredients such as sodium carboxylate, betaine, whey, trimethylglycine, sphingolipids, ceramides, phytosphingosine, cholesterol, cholesterol derivatives, oily ingredients such as phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride ,
Guay Azulene,

Anti-inflammatory agents such as hydrocortisone, vitamins A such as vitamin A, vitamin B ₂ , vitamin B ₆ , vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinic acid amide and vitamin C ester, Allantoin, diisopropylamine dichloroacetate, active ingredients such as 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherols, carotenoids, flavonoids, tannins, lignans and saponins,
Cell activating agents such as α-hydroxy acid and β-hydroxy acid, blood circulation promoting agents such as γ-oryzanol and vitamin E derivatives, wound healing agents such as retinol and retinol derivatives,
Whitening agents such as arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, glutathione, cepharanthin, licorice extract, capsicum tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, Acetic acid DL
-Α-tocopherol, nicotinic acid, nicotinic acid derivative, calcium pantothenate, D-pantothenyl alcohol, acetylpantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol, ethinylestradiol, capronium chloride, benzalkonium chloride, hydrochloric acid Diphenhydramine, tacanal, camphor, salicylic acid, nonyl vanillyl amide, nonanoic vanillyl amide, piroctone olamine, glyceryl pentadecanoate, L-menthol,
Mononitroguaiacol, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, cantharitin tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil, polyoxyethylene sorbitan monostearate, peppermint oil, Sadashiki extract And the like.

(10) Examples of antioxidants Sodium hydrogen sulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolylbiguanide, nordihydroguaiaretinic acid, parahydroxyanisole, butylhydroxyanisole. , Dibutylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoids, flavonoids, tannins, lignans, saponins, apple extracts and plant extracts that have antioxidant effects such as clove extract. .

(11) Examples of solvents Purified water, ethanol, lower alcohols, ethers, L
Examples thereof include PG, fluorocarbon, N-methylpyrrolidone, fluoroalcohol, volatile linear silicone, and next-generation CFC.

Further, existing emulsifiers and the like can be added to the cosmetic composition of the present invention at a general concentration. For example, commentary on the Second Edition of Cosmetic Raw Material Standard, edited by the Japanese Official Book Church, 1
984 (Pharmaceutical Daily), non-standard ingredients for cosmetics, supervision by the Ministry of Health, Labor and Welfare Pharmaceutical Affairs Bureau examination section, 1993 (Yakuji Nippo), supplements for non-standard ingredients for cosmetics, supervision by Ministry of Health, Labor and Welfare Examination Division,
All the drugs listed in 1993 (Yakuji Nippo), permission criteria for each cosmetic type, supervision of the Ministry of Health, Labor and Welfare Pharmaceutical Affairs Bureau Examination Division, 1993 (Yakuji Nippo), and Cosmetic Raw Materials Dictionary, 1991 (Nikko Chemicals), etc. Can be used.

As the cosmetics to which the cosmetic composition of the present invention can be applied, for example, makeup cosmetics such as foundation, white powder, eye shadow, eyeliner, eyebrow, cheek, lipstick, nail color; milky lotion, cream, lotion, Basic cosmetics such as calamine lotion, sunscreen, suntan, aftershave lotion, preshave lotion, pack, acne-preventing cosmetic, essence, etc .; shampoo, conditioner, conditioner, hair color, hair tonic, setting agent, hair restorer, permanent Hair cosmetics such as agents; body powder, deodorant, depilatory, soap, body shampoo, bath salt, hand soap, perfume and the like.

The dosage form of the cosmetic composition of the present invention is not particularly limited, and it may be in the form of two layers, water-in-oil emulsion, oil-in-water emulsion, gel, spray, mousse, oil, solid, sheet or powder. A conventionally known dosage form such as a shape can be used.

The present invention aims to remarkably enhance the storage stability of an iturin-based peptide, which is an environmentally friendly and highly safe bactericidal component, by incorporating it into cosmetics and the like.

Since these can associate with fat-soluble components contained in functional cosmetics, for example, vitamin C derivatives and vitamin E, which are added to enhance antioxidant activity, their physicochemical stability is enhanced. In addition to being able to do so, they can help their cell membrane permeation.

In the present invention, the dermatological agents include, for example, skin milk, skin cream, foundation cream, massage cream, cleansing cream, shaving cream, cleansing foam, lotion, lotion, pack, shampoo, conditioner, hair restorer and hair nourishing agent. , Hair dyes, hair dressings, toothpastes, mouthwashes, permanent wave agents, ointments, bath salts, body soaps, and other cosmetics, to which ingredients effective as quasi-drugs and pharmaceuticals have been added Say. Any type may be used as long as it makes contact with the skin during use. Also, it does not matter the sex or age of the user.

For example, the dermatological agents of the present invention include anti-inflammatory agents salicylic acid derivative type anti-inflammatory agents, aniline derivative type anti-inflammatory agents,
An antispasmodic, a pyrazolone derivative-type anti-inflammatory agent, an indomethacin-based anti-inflammatory agent, a mefenamic acid-based anti-inflammatory agent, an antihistamine, an antiallergic agent, an anti-inflammatory enzyme agent, a steroid agent, glycyrrhizin, azulene, allantoin and the like can be blended. The combined use of these anti-inflammatory agents promotes the anti-inflammatory effect on wounds. The amount used is generally 0.001-1
It may be about 0 mol / L (skin preparation).

Further, the components described in the following raw materials (1) to (6) may be contained. (1) Medical drug Japan Pharmaceutical Collection 21st Edition 1997 (Yakuji Nipposha, 1-210
(0), (2) General Medicines Japan Pharmaceutical Collection 1998-99 (Yakuji Nipposha, issued November 10, 1997, paragraphs 1 to 1100), (3) 13th Amendment Japanese Pharmacopoeia First Supplement (Pharmaceutical Industry) Jikhosha, issued January 31, 1998, 58-19
(0), (4) OTC Japanese Pharmaceuticals Collection 1998-99 (Yakuji Nipposha, issued November 10, 1997, paragraphs 1 to 1100), (5) Laws related to existing additive list due to revision of Food Sanitation Law Notifications (Ministry of Health, Health and Welfare Bureau, Food Chemistry Section, published July 10, 1996, Social Insurance Publisher, 5
-221), (6) List of constituents of feed additives, etc., 8th edition (Japan Chemical Feed Association, published November 18, 1996, paragraphs 7-827).

In the present invention, the dermatological agent can be effectively used for the prevention and treatment of skin diseases such as acne and atopic dermatitis. The dermatological agent of the present invention is generally used as another active ingredient in the agent, such as an anti-acne agent, an anti-androgen, an antibacterial agent, an anti-inflammatory agent, an antioxidant, a radical scavenging agent, and a whitening agent. What is used can be added arbitrarily and can be used together. Anti-androgen active ingredients used in combination are cyproterone acetate, spironolactone, estrogen, glucocorticoid, etc.Antibacterial active ingredients used in combination are erythromycin, clindamycin, gentamicin, penicillin, chloramphenicol, tetracycline. Antibiotics such as benzoyl peroxide, nadifloxacin, ethanol, benzalkonium chloride, sulfur, parahydroxybenzoate esters, salicylic acid, hinokitiol, triclosan, homosulfamine, etc. Examples include ibuprofen piconol, glycyrrhizin, camphor, indomethacin and the like.

Other anti-acne active ingredients to be used in combination include tretinoin, resorcin, isopropylmethylphenol, tocopherol, ascorbic acid and the like. Further examples of the whitening agent active ingredient used in combination include placenta extract, kojic acid, ellagic acid, arbutin, tranexamic acid ester and the like. In addition to this, chamomile extract, kumazasa extract, rose extract, melissa extract, gentian extract, licorice extract, jojoba extract, rosemary extract, sage extract, thyme extract, lavender extract, button extract, carrot extract, aloe extract, soybean extract, perilla extract, Mugwort extract, turmeric extract, hiba extract, cypress extract, rhubarb extract, pearl oyster extract, laurel extract, ginkgo extract, mulberry extract, tea extract, grape skin extract, eleuthero extract, sea chamomile extract and various seaweed extracts, plant-derived antibacterial, Antioxidant and anti-inflammatory components can also be used in combination.

The active ingredients used in combination are added in the range of 0.01% to 50%, depending on the type and use. The dermatological agent in the present invention can be used in any formulation form suitable for the purpose by a known formulation technique together with a formulation carrier. These are, for example, patches,
For plasters, oily ointments, aqueous ointments, plasters, liniments, gels, creams, serums, lotions, emulsions, lotions, packs, plasters, soaps, facial cleansers, body soaps, hair treatments, rinses, etc. is there. As a component to be added for preparing these agents, a component generally generally used can be arbitrarily used. These include, for example, surfactants, oils, alcohols, humectants,
Examples include thickeners, preservatives, antioxidants, chelating agents, pH adjusters, fragrances, dyes, ultraviolet absorbers, ultraviolet scattering agents, amino acids and the like.

The use of the dermatological agent of the present invention is not limited to acne, but is effective for all applicable uses of tretinoin. Its uses include effects on dermatitis and promotion of hair growth. For example, it can be used in a mixed formulation of a hair growth component such as minoxidil and tretinoin. Tretinoin is usually used in the range of 0.05 to 0.1 in Europe and America, but depending on the person, even 0.01% of tretinoin may cause skin irritation and irritation such as eczema.
In such cases, tretinoin 0.1% to 0.0
It is effective to use 01% and the skin preparation of the present invention in an amount of 0.1 to 10% by mass.

Regarding iturin which constitutes the present invention, for example, Bacillus amyloliquefaciens strain disclosed in JP-A-7-143897 and JP-A-2-
The Bacillus subtilis strains disclosed in JP-A-209803 and JP-A-5-51305 can be mentioned. Thus, as a microorganism capable of producing iturin, the Bacillus strain is the most well known, and among them, Bacillus amyloliquefaciens and Bacillus subtilis are famous. Similarly, regarding Surfactin, US Pat.
It is known that Bacillus strains are produced as described in No. 957728, but it is needless to say that iturin that completes the present invention is not limited to these Bacillus strains.

[0066]

EXAMPLES The antibacterial cosmetic composition of the present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

Example 1 Correlation between Iturin Concentration and Antibacterial Effect Iturin was added to the previously prepared culture medium of yeast cells (Saccharomyces cerevisiae) in an amount of 0 to 8 as shown in Table 1.
After mixing in a concentration range of 0 μM and incubating at room temperature (25 ° C.) for 5 hours, the culture solution was filtered with a membrane filter (diameter: 0.22 μm) to absorb the cytoplasmic components contained in the filtrate by ultraviolet absorption (260 nm and 280 nm). ) Was measured. The results are shown in Table 1.

This test is to observe the extent to which the cell membrane of yeast cells was destroyed by the action of iturin, and the numerical values in Table 1 are relative to the maximum value (100%) when the yeast cells were completely destroyed. It means the ratio (%).
Furthermore, the case of adding vitamin E (20 μM) to iturin was also examined. From this result, it is shown that the destruction of yeast cells proceeds depending on the concentration of iturin. Further, since a slight enhancing effect is observed even when vitamin E is added, it is considered that fat-soluble vitamin E is also involved in the micelle structure formation when iturin acts on the cell membrane.

[0069]

[Table 1]

Example 2 Mold Resistance Test Using commercially available iturin (Iturin A: Sigma),
The antifungal performance (mold resistance test; based on JIS Z 2911) was tested. The molds used in the test are as follows.
All of these molds are typical indicator bacteria used in the evaluation of antiseptic systems.

Aspergillus niger ATCC 9642 ・ Penicillium luteum ATCC 9644 ・ Cladosporium herbarum IAM. F517 ・ Trichoderma T-1 ATCC 9645 ・ Chaetomium globosum ATCC 6205

A feed (suspension station containing 100 μM of iturin) was immersed in No. 5 filter paper and dried to prepare a test piece having a diameter of 30 mm. Next, according to JIS Z2911, spray the mixed bacterial suspension of the above 5 species and then
Cultivation was carried out for 3 weeks in the incubator, and the growth of mold on the surface of the test piece was examined (FIG. 1).

In the test, ethyl paraoxybenzoate (0.05%), which is generally used in cosmetics, was used as a positive control and compared with iturin test pieces. It is shown in Table 2.

[0074]

[Table 2]

1 ... Mold growth on the sample surface exceeds 1/3 2 ... Mold growth on the sample surface does not exceed 1/3 3 ... Mold growth is not observed on the sample surface () "○: There is a stop band, ×: There is no stop band"
Indicates.

The processing conditions are as follows. Untreated ··· No neutralization + hot water immersion Neutralization ··· “Non-treated” is neutralized in a high-humidity carbon dioxide atmosphere Hot water immersion ··· “Untreated” is neutralized after dipping in 40 ° C hot water From this result, iturin was found to have excellent antifungal performance.

(Example 3) Antibacterial property test As in Example 2, commercially available iturin was used to evaluate the antibacterial property. Antibacterial activity was evaluated by the minimum inhibitory concentration (MIC) against the microorganisms tested. The results are shown in Table 3. The indicator bacteria used in the test are representative bacterial strains used in the evaluation of the antiseptic system of cosmetics, as well as representative bacterial strains that may cause contamination in daily life.

[0078]

[Table 3]

As a result, it became clear that iturin exerts a broad antibacterial activity at low concentrations.

Example 4 Effect of Vitamin to Increase Skin Permeability TESTS commercially available as a model for reconstructing human skin
Using KIN (Toyobo), L-ascorbyl palmitate (AAP) was administered from the horny layer side, and in the presence or absence of iturin (80 μM), the amount and conversion of ascorbic acid converted after penetrating the skin The L-ascorbic acid palmitate permeated without the treatment was quantified and evaluated.

The test method was as follows: L-ascorbyl palmitate (manufactured by Sigma) 100 and 200 mM solutions were placed on a third model of human skin, allowed to stand at 37 ° C. in the presence of 5% CO ₂ for 2 hours, and then calcium. It was washed with an ion-free phosphate buffer (pH 7.2) and stored frozen. This was homogenized with an acetonitrile solution containing 0.2% phosphoric acid and centrifuged (12,000 rpm × 5 min).
After filtration, the ascorbic acid (nmol / mg protein) contained in the filtrate was quantified by HPLC. The results are shown in Table 4.
Shown in.

[0082]

[Table 4]

As a result, it became clear for the first time that iturin has an effect of assisting skin penetration of L-ascorbyl palmitate. It is considered that this is because the lipid-soluble site of L-ascorbyl palmitate ester interacts with the micelle formed by iturin to protect its oxidative modification.

Example 5 Preparation of Antibacterial Cosmetic (Cream) A cream was prepared according to the following formulation. That is, (1) to (6),
(10) was melted by heating and kept at 70 ° C (oil phase). To this (7)
Add while stirring to dissolve. Next, change (8) to (9) to (11)
While stirring, add the oil phase to the one that was heated and dissolved.
After treatment with a homomixer, it was rapidly cooled to obtain a cream.

Hereinafter, the compounding composition values represent% by mass. (1) Vaseline 8.0 (2) Lanolin 2.0 (3) Squalane 20.0 (4) Cetanol 5.0 (5) Glycerol monostearate 2.0 (6) Polyoxyethylene sorbitan monolaurate (20EO) 2.0 (7) Aitulin 0.7 (8) Glycerol (86%) 5.0 (9) 1,3-butylene glycol 5.0 (10) Perfume 0.1 (11) Purified water 50.2

Example 6 Preparation of Antibacterial Cosmetic (Lotion) Lotion was prepared according to the following formulation. That is, the components (1) to (6) are sequentially added to (7) and mixed uniformly to adjust.

[0087] (1) Ethanol 0.5 (2) 1,3-butylene glycol 10.0 (3) Glycerin 2.0 (4) Polyoxyethylene hydrogenated castor oil 0.6 (5) Iturin 1.0 (6) Fragrance 0.06 (7) Purified water 85.84

Example 7 Preparation of Antibacterial Cosmetic (Emulsion) An emulsion was prepared according to the following formulation. That is, the oil phases (1) to (6) are mixed, heated and melted and kept at 75 ° C. On the other hand, (7) ~ (1
The aqueous phase (0) is mixed, dissolved by heating to 75 ° C., and the above oil phase is added thereto with stirring to emulsify. 40 ℃ after cooling
At (11) and (12) are added and mixed.

[0089] (1) Squalane 4.0 (2) Glyceryl tri-2-ethylhexanoate 2.0 (3) Cetyl 2-ethylhexanoate 3.0 (4) Cetanol 0.6 (5) Stearyl alcohol 0.4 (6) Sorbitan monostearate 1.2 (7) 1,3-butylene glycol 6.0 (8) Dipropylene glycol 4.0 (9) Polyoxyethylene (20EO) sorbitan monostearate ester 8 (10) Purified water 76.4 (11) Iturin 1.5 (12) Perfume 0.1

Example 8 Preparation of Antibacterial Cosmetic (Makeup Base Cream) A makeup base cream was prepared according to the following formulation. That is, (10) ~ (12) is kneaded in (4), this (5) ~ (7)
Is added to the aqueous phase, mixed and heated to 70 ° C. On the other hand, (1)
The oil phase components (3) to (3) are mixed and heated to 70 ° C., and this is added to the aqueous phase with stirring to emulsify. After emulsification, the mixture is cooled and (8) to (9) are added at 40 ° C.

[0091] (1) Stearic acid 12.0 (2) Cetanol 2.0 (3) Self-emulsifying glyceryl monostearate 2.0 (4) Propylene glycol 10.0 (5) Glycerin 3.0 (6) Potassium hydroxide 0.3 (7) Purified water 67.6 (8) Iturin 1.5 (9) Perfume 0.1 (10) Titanium dioxide 1.0 (11) Bengala 0.1 (12) Yellow iron oxide 0.4

(Example 9) Preparation of antibacterial cosmetic (milky liquid foundation) A milky liquid foundation was prepared by the following formulation. That is, the pigments (15) to (19) are mixed and then pulverized by a pulverizer. (12) is heated to 70 ° C., (9) is added to swell well, (8) is dispersed in (10) in advance, and (11) is further added and dissolved. The oil phases (1) to (7) are mixed, heated and melted to 80 ° C. The pigment is added to the aqueous phase with stirring, and the mixture is passed through a colloid mill to 75 ° C., the oil phase is added with stirring to emulsify, and after cooling 40
(13) to (14) are added at ℃.

[0093] (1) Stearic acid 2.4 (2) Propylene glycol monostearate 2.0 (3) Cetostearyl alcohol 0.2 (4) Liquid lanolin 2.0 (5) Liquid paraffin 3.0 (6) Isopropyl myristate 8.5 (7) Glyceryl monostearyl ether 3.5 (8) Carboxymethyl cellulose sodium 0.2 (9) Bentonite 0.5 (10) Isoprene glycol 4.0 (11) Triethanolamine 1.1 (12) Purified water 52.5 (13) Aitulin 2.0 (14) Perfume 0.1 (15) Titanium oxide 8.0 (16) Talc 4.0 (17) Bengala 3.0 (18) Yellow iron oxide 2.5 (19) Black iron oxide 0.5

Example 10 Preparation of Antibacterial Cosmetic (Cream Foundation) A cream foundation was prepared according to the following formulation. That is, the pigments (13) to (19) are mixed and then pulverized by a pulverizer. Mix (7) to (10), dissolve and heat. (1) ~
The oil phase of (6) is mixed, heated and dissolved to 80 ° C. The pigment is added to the aqueous phase with stirring, the temperature is raised to 75 ° C. through a colloid mill, the oil phase is added with stirring to emulsify, and after cooling, (11) to (12) are added at 40 ° C.

[0095] (1) Stearic acid 5.0 (2) Lipophilic glyceryl monostearate 2.5 (3) Propylene glycol monolaurate 3.0 (4) cetostearyl alcohol 1.0 (5) Liquid paraffin 7.0 (6) Isopropyl myristate 8.0 (7) Diglycerin 3.0 (8) Triethylene glycol 2.0 (9) Triethanolamine 1.2 (10) Purified water 44.2 (11) Iturin 2.0 (12) Perfume 0.1 (13) Titanium oxide 8.0 (14) Kaolin 5.0 (15) Talc 2.0 (16) Bentonite 1.0 (17) Bengalla 2.6 (18) Yellow iron oxide 2.1 (19) Black iron oxide 0.3

Example 11 Preparation of Antibacterial Cosmetic (Emulsion Eye Color) An emulsion eye color was prepared according to the following formulation. That is,
The aqueous phases (5) to (8) were mixed, dissolved and heated, and (12) and (13) which had been mixed and pulverized in advance were added to and dispersed in the aqueous phase, and the temperature was 75 ° C.
Heat to. Premixed and heated to make it uniform (1) to (4) with stirring to emulsify and after cooling (9)
~ (11) are added and mixed.

[0097] (1) Stearic acid 8.0 (2) White petrolatum 15.0 (3) Isopropyl palmitate 5.0 (4) Lanolin 5.0 (5) 1,3-butylene glycol 5.0 (6) Hexylene glycol 5.0 (7) Triethanolamine 2.0 (8) Purified water 51.08 (9) Iturin 1.0 (10) Lavender ethanol extract 1.25 (11) Perfume 0.15 (12) Red 221 0.02 (13) Ultramarine 1.5

Example 12 Preparation of Antibacterial Cosmetic (Emulsified Cheek Color) An emulsified cheek color was prepared according to the following formulation. That is,
The aqueous phases (11) to (13) are mixed, dissolved and heated, and (16) and (17) previously mixed and pulverized are added to and dispersed in the mixture.
Heat to ℃. Premixed and heated to make uniform (1) to (10) with stirring to emulsify and after cooling
(14) to (15) are added and mixed.

[0099] (1) Beeswax 3.0 (2) Stearic acid 2.0 (3) Cetanol 3.0 (4) Lanolin 3.0 (5) Liquid paraffin 15.0 (6) Isopropyl myristate 7.0 (7) Polyoxyethylene (20EO) sorbitan monostearate 4.2 (8) Sorbitan monostearate 1.8 (9) Glyceryl monostearate 2.0 (10) Glyceryl monopalmityl ether 2.0 (11) Propylene glycol 5.0 (12) Triethanolamine 0.6 (13) Purified water 46.95 (14) Iturin 2.0 (15) Perfume 0.15 (16) Red No. 202 0.05 (17) Yellow iron oxide 2.25

(Example 13) Preparation of antibacterial cosmetics (emulsion type eyeliner) An emulsion type eyeliner was prepared by the following formulation. That is,
The oil phase components (1) to (4) are mixed and heated to dissolve them. The aqueous phases (5) to (8) are mixed with this, heated, and added with stirring to emulsify. Next, add (11) to (13) to this emulsion, disperse through a colloid mill, cool, and then at 40 ° C.
Add (9) to (10).

[0101] (1) Stearic acid 3.5 (2) Beeswax 2.0 (3) Carnavalou 0.5 (4) Microcrystalline wax 5.0 (5) 1,3-butylene glycol 7.0 (6) Ethylene glycol monobutyl ether 2.5 (7) Triethanolamine 1.5 (8) Purified water 45.9 (9) Iturin 2.0 (10) Perfume 0.1 (11) 3.0% by mass bentonite extract 20.0 (12) Titanium oxide 8.0 (13) Carbon black 2.0

Example 14 Preparation of Antibacterial Cosmetic (Aqueous Suspension Mascara) An aqueous suspension mascara was prepared according to the following formulation. That is,
(2) to (6) are added to and dissolved in (9), and then (7) to (8) are added and dispersed through a colloid mill. To this (1)
Add and disperse evenly.

[0103] (1) 50% by mass vinyl acetate emulsion 37.0 (2) Sodium carboxymethyl cellulose 1.0 (3) 1,3-butylene glycol 3.5 (4) Ethylene glycol monomethyl ether 3.5 (5) Iturin 1.0 (6) Titanium oxide 8.0 (7) Carbon black 1.6 (8) Bengala 0.4 (9) Purified water 44.0

Example 15 Preparation of Antibacterial Cosmetic (Cleansing Gel) A cleansing gel was prepared according to the following formulation. That is, after adding (3) and (7) to (11) to make them uniform, they are heated to 70 ° C. and uniformly dissolved. Then, after cooling, (9) to (10) are added at 40 ° C., and finally (8) is added to neutralize.

[0105] (1) Glycerin 15.0 (2) 1,3-butylene glycol 10.0 (3) Silicic anhydride 7.0 (4) Polyoxyethylene (20EO) lauryl ether 5.0 (5) Polyoxyethylene (20EO) hydrogenated castor oil 2.5 (6) Diethylene glycol monoethyl ether 5.0 (7) Carboxyvinyl polymer 0.5 (8) Potassium hydroxide 0.45 (9) Iturin 2.0 (10) Perfume 0.1 (11) Purified water 52.45

(Example 16) Preparation of antibacterial cosmetics (hair rinse) Hair rinse was prepared according to the following formulation. That is, in (9)
Add (5) and (7) and heat to 70 ° C. On the other hand, (1) to (4) are mixed, dissolved and heated to 70 ° C. While stirring this oil phase, gradually add it to the previously prepared aqueous phase to pre-emulsify it, add a homomixer to homogenize it, and then cool at 40 ° C.
Add (6) and (8).

[0107] (1) Cetanol 3.0 (2) Stearyl trimethyl ammonium chloride 0.7 (3) Silicone oil 3.0 (4) Polyoxyethylene (10EO) oleyl ether 1.0 (5) Glycerin 5.0 (6) Iturin 1.5 (7) Green No. 3 1% by mass aqueous solution 0.2 (8) Perfume 0.1 (9) Purified water 85.5

Example 17 Preparation of Antibacterial Cosmetic (Hair Treatment) Hair treatment was prepared according to the following formulation. That is,
The oil phase components (1) to (7) are mixed and heated to 80 ° C. On the other hand, the water phase components (8) to (10) are mixed and heated to 85 ° C., the oil phase is added to this to emulsify, and after cooling, 40 ° C.
(11) to (12) are added at.

[0109] (1) Polyoxyethylene (30EO) behenyl ether 4.0 (2) Self-emulsifying glyceryl monostearate 6.0 (3) Isopropyl myristate 5.0 (4) Hexyldecanol 5.0 (5) Squalane 3.0 (6) Purified lanolin 3.0 (7) Stearic acid 5.0 (8) Lauryl dimethylamino acetic acid betaine 5.0 (9) Glycerin 10.0 (10) Purified water 52.8 (11) Perfume 0.2 (12) Iturin 1.0

(Example 18) Preparation of antibacterial cosmetic (facial cleanser) A facial cleanser was prepared according to the following formulation. That is, the oil phase of (1) to (6) and the water phase of (7) and (8) are mixed and melted at 65 ° C., respectively, and then melted, and then the water phase is added to the oil phase to emulsify. After cooling, 40 ℃
Then, (9) to (10) are added and mixed.

[0111] (1) Liquid paraffin 25.0 (2) Cetanol 2.0 (3) Lanolin 2.0 (4) Glyceryl monostearate 5.0 (5) Polyoxyethylene stearyl ether 5.0 (6) Polyoxyethylene (20EO) sorbitan monostearate 1.0 (7) 1,3-butylene glycol 10.0 (8) Purified water 48.4 (9) Perfume 0.1 (10) Iturin 1.5

Example 19 Preparation of Antibacterial Cosmetic (Body Shampoo) A body shampoo was prepared according to the following formulation. That is,
The components (1) to (8) are sequentially added to (9) and mixed uniformly.

[0113] (1) Sodium lauryl ether sulfate 20.0 (2) Myristyl ether sodium sulfate 10.0 (3) Capric acid diethanolamide 3.0 (4) Polyoxypropylene (10PO) Polyoxyethylene (20EO) cetyl ether                                                            3.5 (5) Sodium chloride 2.5 (6) Glycerin 10.0 (7) Aitulin 0.5 (8) Perfume 0.3 (9) Purified water 50.2

Next, the above Examples 5 to 19 were evaluated for antibacterial activity, skin irritation and discomfort during use.

(1) Evaluation of antibacterial activity As bacteria, Escherichia coli, Staphylococcus aureus and Pseudomonas
aeruginosa) as a fungus, Candida albica
ns) and black mold (Asperugillus niger) were used to inoculate 10 ⁶ bacteria and 10 ⁵ fungi per 1 g of the sample, and 3
After culturing at 7 ° C and 25 ° C respectively, two weeks later, when the bacteria were killed, the viable count was 1/1 for the fungi.
When it was 000 or less, it was judged as a pass. In addition, the antibacterial activity test results are those that passed in the table are "○",
Those that failed were indicated as “x”.

[0116]

[Table 5]

As is clear from Table 5, in Examples 5 to 19 of the present invention, sufficient antibacterial activity against both bacteria and fungi was observed.

(2) Evaluation of skin irritation For Examples 5 to 19, a 35-hour male patcher was used for a 48-hour occlusion patch test, and the skin irritation index of 35 people was evaluated by the criteria shown in Table 6. Was calculated. In addition, about Examples 15-19, 1.0 mass%
The aqueous solution was used for the test.

[0119]

[Table 6]

(3) Evaluation of discomfort during use 20 female panelists were set as one group, and each group was treated with Examples 5 to 19
Each of them was used and evaluated for discomfort such as a tingling sensation, tingling sensation, and tingling sensation during 30 seconds to 1 minute after application.

The evaluation result is "I feel very strong" = 5 points "Slightly strong" = 4 points "Feel" = 3 points "Feel a little" = 2 points "I feel delicate" = 1 point "I don't feel" = 0 points Was evaluated and shown as the average value of 20 people.

At this time, for Examples 15 to 19, 1.
A 0 mass% aqueous solution was used for the test. The results are summarized in the table below.

[Table 7]

From Table 7, in any of Examples 5 to 19 of the present invention, no irritation to the skin disease preventive / therapeutic agent was observed, and discomfort during use was slightly felt. Thus, according to the present invention, it is possible to obtain an antibacterial hypoallergenic cosmetic composition which has an enhanced antibacterial effect and is not only irritating to the skin, but also hardly causes discomfort during use.

[0124]

EFFECTS OF THE INVENTION The antibacterial cosmetic composition of the present invention dramatically improves the stability of cosmetics against microbial contamination by the iturin-based peptide, which is extremely safe for humans and the environment. It is possible to enhance the skin permeability of the fat-soluble functional ingredient of and enhance its effect.

[0125]

[Brief description of drawings]

FIG. 1 is an apparatus diagram showing an example of a mold growth test using the antibacterial cosmetic composition of the present invention.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 7/032 A61K 7/032 7/035 7/035 7/06 7/06 7/08 7/08 7 / 50 7/50 (72) Inventor Satoshi Tsugi, 1-1 1-1 Onodai, Midori-ku, Chiba, Chiba Prefecture, Showa Denko K.K. (72) Eiji Ogata 1-1, Onodai, Midori-ku, Chiba, Chiba Prefecture No. 1 Showa Denko Co., Ltd. Research Institute F-term (reference) 4C083 AA082 AA112 AB032 AB232 AB242 AB332 AB432 AB442 AC012 AC022 AC072 AC102 AC122 AC172 AC242 AC342 AC392 AC422 AC432 AC442 AC542 AC642 AC692 AC792 AD092 AD112 AD152 AD272 AD411 AD412 AD611 CC02 CC04 CC04 CC04 CC04 CC04 CC04 CC04 CC04 CC11 CC12 CC14 CC23 CC33 DD27 DD32 DD33 DD41 EE14

Claims (6)

[Claims] 1. An antibacterial cosmetic composition comprising an iturin peptide as an active ingredient. 2. The antibacterial cosmetic composition according to claim 1, wherein the iturin peptide is a compound represented by the general formula (1). [Chemical 1] (In the formula (1), R represents a linear or branched alkyl group having 3 to 10 carbon chains.) 3. The antibacterial cosmetic composition according to claim 1 or 2, which contains a fat-soluble vitamin or a derivative thereof. 4. A cosmetic containing the antibacterial cosmetic composition according to any one of claims 1 to 3. 5. A skin preparation containing the antibacterial cosmetic composition according to any one of claims 1 to 3. 6. The skin preparation according to claim 5, which is a prophylactic or therapeutic agent for acne and atopic dermatitis.