JP2003102300A - Medium for plant, plant culture apparatus and method for plant culturing - Google Patents
Medium for plant, plant culture apparatus and method for plant culturingInfo
- Publication number
- JP2003102300A JP2003102300A JP2001303572A JP2001303572A JP2003102300A JP 2003102300 A JP2003102300 A JP 2003102300A JP 2001303572 A JP2001303572 A JP 2001303572A JP 2001303572 A JP2001303572 A JP 2001303572A JP 2003102300 A JP2003102300 A JP 2003102300A
- Authority
- JP
- Japan
- Prior art keywords
- plant
- medium
- culture
- cell tray
- horticultural soil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012258 culturing Methods 0.000 title claims description 9
- 238000000034 method Methods 0.000 title claims description 8
- 239000002609 medium Substances 0.000 claims abstract description 31
- 239000002689 soil Substances 0.000 claims abstract description 21
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000006870 ms-medium Substances 0.000 abstract description 5
- 241000196324 Embryophyta Species 0.000 description 43
- 230000012010 growth Effects 0.000 description 19
- 239000003415 peat Substances 0.000 description 15
- 239000010455 vermiculite Substances 0.000 description 13
- 235000019354 vermiculite Nutrition 0.000 description 13
- 229910052902 vermiculite Inorganic materials 0.000 description 13
- 229920001817 Agar Polymers 0.000 description 10
- 239000008272 agar Substances 0.000 description 10
- SHFGJEQAOUMGJM-UHFFFAOYSA-N dialuminum dipotassium disodium dioxosilane iron(3+) oxocalcium oxomagnesium oxygen(2-) Chemical compound [O--].[O--].[O--].[O--].[O--].[O--].[O--].[O--].[Na+].[Na+].[Al+3].[Al+3].[K+].[K+].[Fe+3].[Fe+3].O=[Mg].O=[Ca].O=[Si]=O SHFGJEQAOUMGJM-UHFFFAOYSA-N 0.000 description 9
- 239000010451 perlite Substances 0.000 description 8
- 235000019362 perlite Nutrition 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 6
- 238000011109 contamination Methods 0.000 description 6
- 241000207748 Petunia Species 0.000 description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
- 229930006000 Sucrose Natural products 0.000 description 5
- 239000005720 sucrose Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 238000012136 culture method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 206010021033 Hypomenorrhoea Diseases 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000003595 mist Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 230000002786 root growth Effects 0.000 description 2
- 102100022749 Aminopeptidase N Human genes 0.000 description 1
- 102100032768 Complement receptor type 2 Human genes 0.000 description 1
- 101000757160 Homo sapiens Aminopeptidase N Proteins 0.000 description 1
- 101000941929 Homo sapiens Complement receptor type 2 Proteins 0.000 description 1
- 101000935040 Homo sapiens Integrin beta-2 Proteins 0.000 description 1
- 101000914514 Homo sapiens T-cell-specific surface glycoprotein CD28 Proteins 0.000 description 1
- 102100025390 Integrin beta-2 Human genes 0.000 description 1
- 102000003729 Neprilysin Human genes 0.000 description 1
- 108090000028 Neprilysin Proteins 0.000 description 1
- 102100027213 T-cell-specific surface glycoprotein CD28 Human genes 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000004388 gamma ray sterilization Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、植物用培地及び植
物用培養装置並びに植物用培養方法に関する。TECHNICAL FIELD The present invention relates to a plant culture medium, a plant culture device, and a plant culture method.
【０００２】[0002]
【発明が解決しようとする課題】従来より、培養室で一
定量（大きさ）に増殖させた植物体の切片を植物用培
地、例えば寒天培地に挿して培養し、発根させることに
より組織培養苗を生産することが行われている。発根し
た培養苗は、温室内の調整ピートモスやバーミキュライ
トなどの培地で順化させた後、露地或いは温室内の土壌
に移植される。Conventionally, tissue culture is performed by inserting a slice of a plant body grown in a constant amount (size) in a culture room into a plant medium, for example, an agar medium, and culturing and rooting. Producing seedlings is taking place. The rooted cultured seedlings are acclimated in a medium such as conditioned peat moss or vermiculite in a greenhouse and then transplanted to the open field or soil in the greenhouse.
【０００３】ところで、上記した植物組織培養では、雑
菌による汚染が培地に発生して生育率が低下したり枯死
することを防止するため、増殖及び発根のステージ（工
程）における植え付け作業は無菌室（例えばクリーンベ
ンチ）内で行われる。従って、培養苗の生産性を上げる
ためには無菌室を増設する必要がある。By the way, in the above-mentioned plant tissue culture, in order to prevent the growth of the medium from being contaminated by miscellaneous bacteria and thus the growth rate is reduced or the plant is killed, the planting work in the stage of growth and rooting is carried out in a sterile room. (Eg clean bench). Therefore, it is necessary to add a sterile room to increase the productivity of the cultured seedlings.
【０００４】特に、発根のステージは増殖のステージに
比べて無菌室の使用時間が長く、しかも広い作業スペー
スが必要となる。例えば、図５はペチュニアの培養苗を
１００００本生産する場合（増殖率５倍）の増殖及び発
根の各ステージにおける苗数とクリーンベンチの使用時
間を示す図である。この図５に示すように、発根のステ
ージにおけるクリーンベンチの使用時間は全体の約７５
％を占めている。In particular, the rooting stage requires a longer period of use of the sterile room than the growth stage, and requires a large working space. For example, FIG. 5 is a diagram showing the number of seedlings at each stage of growth and rooting and the use time of the clean bench when 10,000 cultured seedlings of petunia are produced (growth rate 5 times). As shown in Fig. 5, the usage time of the clean bench at the rooting stage is about 75% of the total.
Account for%.
【０００５】そこで、発根のステージを短縮することに
より無菌室の増設を伴わずに生産性の向上を図ることが
考えられる。しかし、この場合は、培養苗の順化時の枯
死率が高くなるという問題がある。Therefore, it is conceivable to improve the productivity by shortening the rooting stage without adding a sterile room. However, in this case, there is a problem that the mortality rate of the cultivated seedlings during acclimation becomes high.
【０００６】本発明は上記事情を鑑みてなされたもので
あり、その目的は、無菌室を使用することなく植物切片
を良好に発根及び生育させることができる植物用培地及
び植物培養装置並びに植物培養方法を提供するにある。The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a plant culture medium, a plant culture apparatus, and a plant capable of properly rooting and growing plant slices without using a sterile room. It is to provide a culture method.
【０００７】[0007]
【課題を解決するための手段】本発明の植物用培地は、
滅菌処理された園芸用土に糖を前記園芸用土１ｇ当たり
０〜０．００９５ｇの割合で混合して構成したところに
特徴を有する。上記構成によれば、園芸用土を滅菌処理
すると共に糖分濃度を低く抑えたことにより、オープン
条件下で植物切片を植え付けて培養しても雑菌による汚
染の発生を少なく抑えることができるため、植物切片を
良好に発根及び生育させることができる。The plant medium of the present invention comprises:
It is characterized in that sugar is mixed with sterilized horticultural soil at a ratio of 0 to 0.0095 g per 1 g of the horticultural soil. According to the above configuration, by sterilizing the horticultural soil and suppressing the sugar concentration to a low level, it is possible to suppress the occurrence of contamination by various bacteria even if the plant slice is planted and cultured under open conditions. Can be rooted and grown well.
【０００８】この場合、前記園芸用土を高圧蒸気滅菌処
理するように構成すると、園芸用土に適度な湿度を与え
つつ滅菌することができる。In this case, if the horticultural soil is subjected to high-pressure steam sterilization, the horticultural soil can be sterilized while providing appropriate humidity.
【０００９】また、本発明の植物用培養装置は、ケース
本体及びこのケース本体に着脱可能な蓋からなるケース
と、前記ケース本体内に配置され多数の個室を有するセ
ルトレイとからなる培養容器を備え、上記植物用培地を
前記セルトレイの各個室に充填して構成したところに特
徴を有する。Further, the plant culture device of the present invention comprises a culture container comprising a case body and a case detachable from the case body, and a cell tray arranged in the case body and having a large number of individual chambers. It is characterized in that the above-mentioned plant medium is filled in each cell of the cell tray.
【００１０】上記構成においては、オープン条件下でセ
ルトレイの各個室内の植物用培地に無菌培養植物体の切
片を植え付けて発根させる。そして、発根後、ケースか
らセルトレイを取り出して順化環境に移動させることに
より発根工程から順化工程に移行される。In the above-mentioned structure, under the open condition, a root of the plant culture medium is planted in a plant medium in each compartment of the cell tray and rooted. Then, after rooting, the cell tray is taken out from the case and moved to the acclimation environment, whereby the rooting step shifts to the acclimation step.
【００１１】[0011]
【発明の実施の形態】以下、本発明の実施の形態を詳し
く説明する。本発明に係る植物用培地に用いられる園芸
用土とは、基本用土として知られている調整ピートモス
の他、バーミキュライトやパーライト、及び複数種の園
芸用土を混合したものを含んでいる。用いる園芸用土
は、培養する植物体の種類に応じて適宜、選択すると良
い。BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described in detail below. The horticultural soil used in the plant medium according to the present invention includes adjusted peat moss known as basic soil, vermiculite, perlite, and a mixture of plural types of horticultural soil. The horticultural soil to be used may be appropriately selected depending on the type of plant to be cultured.
【００１２】また、本発明の植物用培地には糖が園芸用
土１ｇ当たり０〜０．００９５ｇの割合で添加されてい
る。この場合、糖分濃度を１ｇ／ｌ以下に調整したＭＳ
(Murashige & Skoog) 培地或いは無糖のＭＳ培地を、園
芸用土１ｇ当たり９．５ｍｌ程度が添加することにより
前記植物用培地を構成することができる。また、ＭＳ培
地に限らず、植物体の組織培養に一般的に用いられる他
の液状の培地やHyponex （商品名）等の液肥を用いるこ
とができる。Further, sugar is added to the plant medium of the present invention at a rate of 0 to 0.0095 g per 1 g of horticultural soil. In this case, MS with a sugar concentration adjusted to 1 g / l or less
The plant medium can be constituted by adding (Murashige & Skoog) medium or sugar-free MS medium in an amount of about 9.5 ml per 1 g of horticultural soil. Further, not limited to MS medium, other liquid medium generally used for tissue culture of plants and liquid fertilizer such as Hyponex (trade name) can be used.
【００１３】本発明の植物用培地の滅菌処理方法は、オ
ートクレーブを用いた高圧蒸気滅菌処理が好ましいが、
乾熱滅菌処理、ガンマ線滅菌処理、化学滅菌処理等の適
宜の方法を用いることができる。また、園芸用土を単独
で滅菌処理しても良いが、培養装置全体を滅菌処理する
ようにしても良い。The plant medium sterilization method of the present invention is preferably high-pressure steam sterilization using an autoclave,
Appropriate methods such as dry heat sterilization treatment, gamma ray sterilization treatment, and chemical sterilization treatment can be used. Further, although the horticultural soil may be sterilized by itself, the entire culture device may be sterilized.
【００１４】本発明の植物用培養装置に用いる培養容器
は、ケース本体及びこのケース本体に着脱可能な蓋から
なるケースと、前記ケース本体内に配置され多数の個室
を有するセルトレイとを備えて構成されている。培養容
器の材質は、ガラスやプラスチック等、特に制限される
ものではないが、十分な光が透過する光透過性を有する
と良い。また、滅菌処理の方法に応じた材質（例えば高
圧蒸気滅菌処理を行う場合には、耐熱性に優れた材質、
また、化学滅菌処理を行う場合は耐薬品性に優れた材
質）から培養容器を構成することが好ましい。The culture container used in the plant culture apparatus of the present invention comprises a case body and a case which is attachable to and detachable from the case body, and a cell tray arranged in the case body and having a large number of individual chambers. Has been done. The material of the culture container is not particularly limited, such as glass and plastic, but it is preferable that the culture container has sufficient light transmissivity to transmit light. In addition, a material suitable for the sterilization method (for example, when performing high-pressure steam sterilization, a material having excellent heat resistance,
Further, when the chemical sterilization treatment is performed, it is preferable to configure the culture vessel from a material having excellent chemical resistance).
【００１５】本発明に係る培養方法は、培養容器のセル
トレイに充填された植物用培地に、無菌的に増殖された
植物体の切片を植え付けて培養するものである。この場
合、ケース本体にセルトレイを配置した後、蓋を装着す
ることによりケース内への雑菌の混入を防止することが
でき、しかも、滅菌処理した園芸用土から植物用培地を
構成したので、オープン条件下で植え付けても良好に発
根させることができる。オープン条件下の好ましい形態
は、室内の例えば培養室内であるが、これに限定される
ものではない。また、本発明の植物用培地は寒天培地に
比べて通気性が非常に優れ発根及び生育状態が非常に良
好となるため、順化工程の省略或いは期間の短縮が可能
となる。The culturing method according to the present invention comprises culturing by assembling a section of a plant body that has been aseptically grown in a plant medium filled in a cell tray of a culture container. In this case, after arranging the cell tray on the case body, it is possible to prevent contamination of miscellaneous bacteria into the case by mounting the lid, and furthermore, since the plant medium was composed of sterilized horticultural soil, open conditions Even if planted below, it can be rooted well. A preferable form under open conditions is, for example, but not limited to, a room inside a culture room. In addition, the plant medium of the present invention has much better air permeability than the agar medium and has very good rooting and growth conditions, so that the acclimation step can be omitted or the period can be shortened.
【００１６】更に、上記培養方法では、オープン条件下
で植付け作業を行う際に、植物体を手で持ち、ハサミで
適宜の大きさの切片に切断して植え付け作業を行って
も、良好に発根することが確認されている。従って、無
菌室内でピンセットやメスを用いて植付け作業を行って
いた従来の培養方法に比べて、作業効率を向上させるこ
とができる。Further, in the above culturing method, when the planting work is carried out under the open condition, even if the plant body is held by hand and cut into pieces of appropriate size with scissors, the planting work is well carried out. It has been confirmed to be rooted. Therefore, the work efficiency can be improved as compared with the conventional culture method in which planting work is performed using tweezers or a scalpel in a sterile room.
【００１７】[0017]
【実施例】以下、実施例を挙げて本発明を詳細に説明す
るが、この発明はこれらの実施例に限定されるものでは
ない。The present invention is described in detail below with reference to examples, but the present invention is not limited to these examples.
【００１８】（第１実施例）図１は本実施例に係る植物
用培養容器を示すものであり、培養容器１は、ＰＰ製の
セルトレイ２と、ＰＰフィラー製のケース本体３及びこ
のケース本体３に着脱可能に装着されるＰＦ製の蓋４か
らなるケース５とから構成されている。(First Embodiment) FIG. 1 shows a plant culture container according to this embodiment. The culture container 1 comprises a cell tray 2 made of PP, a case body 3 made of PP filler, and this case body. 3 and a case 5 composed of a PF lid 4 which is detachably attached.
【００１９】前記セルトレイ２は、トレイ２ａ内を複数
の仕切板２ｂで仕切ることにより構成された複数の個室
（セル）２ｃを有している。従って、仕切板２ｂの組み
合わせを変更することにより適宜の大きさのセル２ｃを
設けることができる。そして、前記セル２ｃ内に植物用
培地（図示せず）を充填することにより植物用培養装置
が構成される。The cell tray 2 has a plurality of individual chambers (cells) 2c formed by partitioning the inside of the tray 2a with a plurality of partition plates 2b. Therefore, the cells 2c having an appropriate size can be provided by changing the combination of the partition plates 2b. Then, a plant culture device is constructed by filling the cell 2c with a plant medium (not shown).
【００２０】前記植物用培地は、園芸用土、具体的には
調整ピートモス（商品名：サウスランド）に、ショ糖濃
度が０，１．５，１０，３０ｇ／ｌとなるように調整さ
れたＭＳ（Murashige&Skoog ）培地を混合して構成され
ている。この場合、ＭＳ培地は調整ピートモス１ｇ当た
り約９．５ｍｌ混合されている。The above-mentioned plant medium is MS for horticultural soil, specifically, adjusted peat moss (trade name: Southland), which has a sucrose concentration of 0, 1.5, 10, 30 g / l. (Murashige & Skoog) medium is mixed. In this case, the MS medium is mixed at about 9.5 ml per 1 g of the prepared peat moss.
【００２１】前記培養装置を、オートクレーブで１２１
℃、２０分間、高圧滅菌した後、無菌状態で維持してい
るペチュニア（ST186 ）の培養苗を３〜４ｃｍに分割し
て各セル内の培地に植え付け、２５℃、１６時間明期
（明期の光強度は１０００ｌｕｘ）の条件下で３週間培
養した。無菌培養苗の分節作業、植え付け作業及び培養
は、いずれもオープン条件下で行った。The above-mentioned culture device was put in an autoclave 121
After autoclaving at ℃ for 20 minutes, the cultivated seedlings of petunia (ST186) maintained in aseptic condition are divided into 3 to 4 cm and planted in the medium in each cell, and at 25 ℃, 16 hours light period (light period The light intensity was 1000 lux) and the cells were cultured for 3 weeks. The segmentation work, planting work and culture of the aseptic culture seedlings were all performed under open conditions.
【００２２】培養３週間後、培地内の雑菌による汚染の
有無と地上部の生育調査を行った。この結果を図２に示
す。尚、図中の生育状態については、生育良好を＋＋、
生育普通を＋、生育不良（約半数が枯死）を＋−、大半
が枯死を−とする４段階評価で示した。After 3 weeks of culture, the presence or absence of contamination by miscellaneous bacteria in the medium and the growth of the above-ground portion were investigated. The result is shown in FIG. Regarding the growth condition in the figure, good growth is ++,
Normal growth was +, poor growth (about half died) was +-, and most of them were dead in a 4-level evaluation.
【００２３】図２から明らかなように、ショ糖濃度が５
ｇ／ｌ以下に調整されたものでは培地内の汚染の発生は
見られなかった。また、地上部の生育はショ糖濃度が１
ｇ／ｌ以下に調整されたもので良好であった。以上よ
り、ショ糖濃度を１ｇ／ｌ以下に調整することにより、
クリーンベンチを使用しないオープン条件下でも雑菌に
よる汚染が発生することなく、培養苗が良好に生育する
ことがわかった。As is clear from FIG. 2, the sucrose concentration was 5
The thing adjusted to g / l or less did not show the occurrence of contamination in the medium. In addition, the sucrose concentration of the above-ground part is 1
It was good that it was adjusted to g / l or less. From the above, by adjusting the sucrose concentration to 1 g / l or less,
It was found that even under open conditions without using a clean bench, the cultured seedlings grow well without contamination by various bacteria.
【００２４】（第２実施例）この実施例では、植物用培
地の園芸用土として、調整ピートモス（商品名：サウス
ランド）、バーミキュライト（商品名：千代田バーミキ
ュライト）、パーライト（商品名：ネニサンソ）、前記
調整ピートモスとバーミキュライト或いはパーライトと
を種々の混合比で混合したものを用いると共に、ＭＳ培
地を無糖とした。そして、上記した以外は第１実施例と
同様の条件で３週間培養した。また、比較対照区とし
て、園芸用土に代えて寒天培地及びフロリアライト（商
品名）を用いて同様に３週間培養を行った。前記フロリ
アライトは、植物繊維とバーミキュライトを主成分とす
る多孔体からなる植物支持体であり、増殖から成苗まで
連続して使用できる植物支持体とした開発されたもので
ある。(Second Example) In this example, as a horticultural soil for a plant medium, adjusted peat moss (trade name: Southland), vermiculite (trade name: Chiyoda vermiculite), perlite (trade name: Nenisanso), the above A mixture of adjusted peat moss and vermiculite or perlite was used at various mixing ratios, and the MS medium was sugar-free. And it culture | cultivated for 3 weeks on the same conditions as 1st Example except the above. Further, as a comparative control group, instead of the horticultural soil, an agar medium and Florialite (trade name) were used and similarly cultured for 3 weeks. The above-mentioned florialite is a plant support comprising a porous body containing plant fibers and vermiculite as main components, and was developed as a plant support that can be continuously used from growth to adult seedlings.
【００２５】更に、寒天処理区は、寒天を洗い流して調
整ピートモスを充填したセルトレイに移植してミスト室
に移動し、その他の処理区は、そのままの状態でミスト
室に移動して１週間の順化を行った。培養３週間後、及
び順化１週間後の生育調査（地上部の草丈及び根の生育
状態）を行った。この結果を図３に示す。尚、図中の根
の生育状態については、極めて良好（セル全体に根が行
き渡っている状態）を＋＋＋＋、良好を＋＋＋、普通を
＋＋、発根小（不良）を＋とする４段階評価で示した。Further, in the agar treatment area, the agar was washed off, transplanted to a cell tray filled with adjusted peat moss, and moved to the mist chamber, and the other treatment areas were moved to the mist chamber as they were, and the order of 1 week Was made. After 3 weeks of culturing and 1 week of acclimation, growth studies (plant height and root growth in the above-ground part) were carried out. The result is shown in FIG. In addition, about the growth condition of the roots in the figure, it is a four-level evaluation in which extremely good (the condition where the roots are spread over the entire cell) is ++++, good is ++++, normal is ++, and small rooting (poor) is +. Indicated.
【００２６】図３に示すように、培養直後の根の生育状
態は、パーライト及び調整ピートモス＋バーミキュライ
ト（３：７）の処理区を除く処理区で寒天処理区（対照
処理区）と同等かそれよりも良好な結果が得られた。特
に、バーミキュライト及び調整ピートモス＋バーミキュ
ライト（７：３）の処理区では、フロリアライト処理区
と同様の優れた結果が得られた。As shown in FIG. 3, the growth condition of the roots immediately after the culture was the same as that of the agar treatment group (control treatment group) in the treatment groups except the treatment group of perlite and adjusted peat moss + vermiculite (3: 7). Better results have been obtained. Particularly, in the treatment group of vermiculite and the adjusted peat moss + vermiculite (7: 3), excellent results similar to those of the florialite treatment group were obtained.
【００２７】また、草丈は、調整ピートモス単独の処理
区、若しくは調整ピートモスとバーミキュライト或いは
パーライトとを混合した処理区で寒天処理区及びフロリ
アライト処理区よりも良好な結果が得られた。Regarding the plant height, better results were obtained than those in the agar-treated group and the florialite-treated group in the treated group with the adjusted peat moss alone or the mixed group with the adjusted peat moss and vermiculite or perlite.
【００２８】順化後の根の生育状態は、パーライト及び
調整ピートモス＋バーミキュライト（７：３）の処理区
でやや劣るものの、それ以外の処理区では良好であっ
た。特に、バーミキュライト及び調整ピートモス＋パー
ライト（７：３）の処理区では、寒天処理区及びフロリ
アライト処理区と同様の極めて良好な結果が得られた。
また、草丈については培養直後の草丈と同様の傾向が得
られ、調整ピートモス単独の処理区、若しくは調整ピー
トモスとバーミキュライト或いはパーライトとを混合し
た処理区で寒天処理区及びフロリアライト処理区よりも
良好な結果が得られた。The condition of root growth after acclimation was slightly inferior in the treated areas of perlite and adjusted peat moss + vermiculite (7: 3), but good in the other treated areas. Particularly, in the treatment group of vermiculite and the adjusted peat moss + perlite (7: 3), extremely good results similar to those of the agar treatment group and the florialite treatment group were obtained.
Regarding the plant height, the same tendency as that of the plant length immediately after culturing was obtained, and the treated peat moss alone was treated better, or the treated peat moss was mixed with vermiculite or perlite, which was better than the agar treatment and the florialite treatment. Results were obtained.
【００２９】（第３実施例）図４は、第３実施例に係る
培養容器を示している。この培養容器１は、セルトレイ
１１の形状が上記各実施例と異なる。即ち、セルトレイ
１１は、多数の円孔状の個室１２を有して構成されてい
る。このような構成においても、上記した各実施例と同
様の結果が得られる。(Third Embodiment) FIG. 4 shows a culture container according to the third embodiment. The shape of the cell tray 11 of this culture container 1 is different from that of each of the above embodiments. That is, the cell tray 11 is configured to have a large number of circular hole-shaped individual chambers 12. Even with such a configuration, the same result as that of each of the above-described embodiments can be obtained.
【００３０】[0030]
【発明の効果】以上の説明から明らかなように、本発明
の植物用培地を用いることにより、無菌的に増殖された
植物体切片の発根工程をオープン条件下で行うことがで
き、しかも、生育及び発根状態は従来の寒天培地を用い
た場合と同等もしくはそれ以上の良好な結果を得ること
ができる。また、長い作業時間を必要とする発根工程に
おいてクリーンベンチを使用せずにオープン条件下で行
うことができるため、生産性の大幅な向上を図ることが
できる。As is apparent from the above description, by using the plant medium of the present invention, it is possible to carry out the rooting step of the aseptically grown plant piece under open conditions, and With regard to the growth and rooting state, good results equivalent to or better than those when using a conventional agar medium can be obtained. In addition, since the rooting process, which requires a long working time, can be performed under open conditions without using a clean bench, the productivity can be significantly improved.
【図１】本発明の第１実施例に係る培養容器を蓋を取り
除いて示す上面図（ａ）及び蓋を装着して示す縦断側面
図（ｂ）FIG. 1 is a top view (a) showing a culture container according to a first embodiment of the present invention with a lid removed, and a longitudinal side view (b) showing the lid attached.
【図２】ペチュニアの無菌培養苗をオープン条件下で培
養したときの雑菌による汚染の発生の有無と地上部の生
育とショ糖濃度との関係を示す図FIG. 2 is a diagram showing the relationship between the presence or absence of contamination by miscellaneous bacteria, the growth of aboveground parts, and the sucrose concentration when aseptic cultured seedlings of petunia were cultured under open conditions.
【図３】本発明の第２実施例に係るペチュニアの無菌培
養苗の培養後及び順化後の生育調査結果を示す図FIG. 3 is a diagram showing the results of growth investigation of aseptic seedlings of petunia according to the second embodiment of the present invention after culturing and acclimation.
【図４】本発明の第３実施例を示す図１相当図FIG. 4 is a view corresponding to FIG. 1 showing a third embodiment of the present invention.
【図５】従来例を説明するためのものであり、ペチュニ
アの無菌培養苗の各ステージにおける苗数とクリーンベ
ンチの使用時間を示す図FIG. 5 is a view for explaining a conventional example, showing the number of seedlings and the usage time of a clean bench at each stage of aseptic seedlings of petunia.
図中、１は植物用培養容器、２，１１はセルトレイ、２
ｃ，１２は個室、３はケース本体、４は蓋、５はケース
を示す。In the figure, 1 is a culture vessel for plants, 2 and 11 are cell trays, 2
Reference numerals c and 12 denote individual chambers, 3 a case body, 4 a lid, and 5 a case.
───────────────────────────────────────────────────── フロントページの続き Ｆターム(参考） 2B022 AA01 AA05 BA02 BA04 BA11 BA16 DA19 2B030 CD03 CD07 CD10 CD13 CD18 CD21 CD28 ─────────────────────────────────────────────────── ─── Continued front page F-term (reference) 2B022 AA01 AA05 BA02 BA04 BA11 BA16 DA19 2B030 CD03 CD07 CD10 CD13 CD18 CD21 CD28
Claims (4)
用土１ｇ当たり０〜０．００９５ｇの割合で混合してな
る植物用培地。1. A plant culture medium, which is obtained by mixing sugar into sterilized horticultural soil at a ratio of 0 to 0.0095 g per 1 g of the horticultural soil.
ていることを特徴とする請求項１記載の植物用培地。2. The medium for plants according to claim 1, wherein the horticultural soil is subjected to high-pressure steam sterilization treatment.
能な蓋からなるケースと、前記ケース本体内に配置され
多数の個室を有するセルトレイとを備えた培養容器と、 前記セルトレイの各個室に充填された請求項１または２
記載の植物用培地とから構成されていることを特徴とす
る植物用培養装置。3. A case comprising a case body and a lid detachably attached to the case body, a culture container provided with a cell tray arranged in the case body and having a large number of individual chambers, and each individual chamber of the cell tray is filled. Claim 1 or 2
A culture device for plants, comprising the culture medium for plants described above.
前記セルトレイの各個室内の植物用培地に植え付けられ
た無菌培養植物体の切片を、オープン条件下で植え付け
る発根工程と、 発根工程の終了後、前記セルトレイを前記ケースから取
り出して順化環境へ移動させて順化する工程とを有する
ことを特徴とする植物培養方法。4. A plant culture apparatus according to claim 3,
A rooting step of planting, under open conditions, a section of a sterile-cultured plant planted in a plant medium in each of the individual compartments of the cell tray, and after completion of the rooting step, the cell tray is taken out of the case to an acclimated environment. A method of culturing a plant, which comprises a step of moving and acclimatizing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001303572A JP2003102300A (en) | 2001-09-28 | 2001-09-28 | Medium for plant, plant culture apparatus and method for plant culturing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001303572A JP2003102300A (en) | 2001-09-28 | 2001-09-28 | Medium for plant, plant culture apparatus and method for plant culturing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2003102300A true JP2003102300A (en) | 2003-04-08 |
Family
ID=19123637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001303572A Pending JP2003102300A (en) | 2001-09-28 | 2001-09-28 | Medium for plant, plant culture apparatus and method for plant culturing |
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| Country | Link |
|---|---|
| JP (1) | JP2003102300A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2966977B1 (en) | 2013-03-13 | 2021-04-28 | Goldfields Collections Pty Ltd | Method of supplying plants |
| JP2021531001A (en) * | 2018-07-13 | 2021-11-18 | ロウズ ティーシー プロプライアタリー リミテッド | Plant growth system, equipment and methods |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997008938A1 (en) * | 1995-09-05 | 1997-03-13 | Mukoyama Orchids Ltd. | Support for plant culture and method for plant growth |
| WO1998005196A1 (en) * | 1996-08-01 | 1998-02-12 | M & M Laboratory Co., Ltd. | Water-holding carrier for plants |
-
2001
- 2001-09-28 JP JP2001303572A patent/JP2003102300A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997008938A1 (en) * | 1995-09-05 | 1997-03-13 | Mukoyama Orchids Ltd. | Support for plant culture and method for plant growth |
| WO1998005196A1 (en) * | 1996-08-01 | 1998-02-12 | M & M Laboratory Co., Ltd. | Water-holding carrier for plants |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2966977B1 (en) | 2013-03-13 | 2021-04-28 | Goldfields Collections Pty Ltd | Method of supplying plants |
| EP2966977B2 (en) † | 2013-03-13 | 2024-10-23 | Goldfields Collections Pty Ltd | Method of supplying plants |
| JP2021531001A (en) * | 2018-07-13 | 2021-11-18 | ロウズ ティーシー プロプライアタリー リミテッド | Plant growth system, equipment and methods |
| JP7523425B2 (en) | 2018-07-13 | 2024-07-26 | ロウズ ティーシー プロプライアタリー リミテッド | Plant propagation system, device and method |
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