JP2002338464A - Muscular atrophy inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a muscular atrophy inhibitor enabling muscular atrophy to be inhibited significantly as described in the Examples (See the specification) by the intake of proanthocyanidin as the active ingredient thereof, thus bringing good news to persons suffering from muscular atrophy. SOLUTION: This muscular atrophy inhibitor is characterized by comprising proanthocyanidin as the active ingredient; wherein the proanthocyanidin is an extract obtained from the squeezed juice of the seeds, rinds or fruits of grapes.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an agent for suppressing muscle atrophy. More specifically, the present invention provides a muscle atrophy that is difficult to suppress with a vitamin E agent or the like, which is known to have a normal muscle atrophy inhibitory effect, by oral ingestion, for example, occurs after fixation of casts or prolonged bed rest (bedridden The present invention relates to an agent for inhibiting disuse atrophy of skeletal muscle, accompanying aging, muscle atrophy associated with aging, and muscle atrophy associated with microgravity exposure (muscle atrophy in outer space and the like).

[0002]

2. Description of the Related Art The present applicant has previously found that proanthocyanidin has a strong antioxidant action and applied for a patent as an antioxidant containing proanthocyanidin as a main component (Japanese Patent Publication No. 3-7232). Also proanthocyanidins,
It has been found that grape seeds or fruit juice can be efficiently extracted from squeezed cake with water or hydrous alcohol (JP-A-3-200781
And JP-A-11-80148). Proanthocyanidins are a type of polyphenol and have the formula

Embedded image Represented by the formula: flavan-3-ol or flavan-3,4-diol as a structural unit, a group of compounds linked by condensation or polymerization at the 4-6 or 4-8 position, and is present in various plants Is a condensed tannin. Note that the above structural formula shows only a case where condensation is performed at the 4-8 position to facilitate understanding. These are given this name because they produce anthocyanidins such as cyanidin, delphinidin, pelargonidin and the like by acid treatment.

[0003] Then, dimers, trimers,
Tetramers and also proanthocyanidins such as procyanidins, prodelphinidins, properargonidines and the like of polymers of 10 to 30-mers or more, stereoisomers thereof and the like are included. Now, muscular atrophy such as skeletal muscle occurs clinically after a cast or a long-term bed rest at the time of a fracture, etc. Prevention is one of the important issues that must be solved in the field of orthopedic surgery. It is. In addition, degenerative atrophy of skeletal muscle accompanying aging is a problem that cannot be avoided by anyone, and this is one of the causes of fractures in elderly people. On the other hand, muscle atrophy associated with microgravity exposure (muscle atrophy in outer space, etc.)
In the future, it is one of the important issues that humanity must solve in order to stay or live in outer space for a long time. To date, methods of suppressing muscle atrophy include sufficient exercise and rehabilitation, but these alone have a limit in the suppression. On the other hand, as a muscle atrophy inhibitor, vitamin E as an antioxidant or deferoxamine as an iron chelator is known, but their effects were not sufficient (Ayumi of Medicine, Vol. 193, No.
7, 2000, Medical and Dental Publishing, Kondo H., Acta. Physio
l. Sscand., 142, 527-528 (1991)).

[0004]

SUMMARY OF THE INVENTION Under the circumstances described above, the present invention aims to overcome the current situation where there is no drastic solution to the suppression of muscle atrophy, and to widely provide a muscle atrophy inhibitor having a clear effect. It was made for the purpose.

[0005]

Means for Solving the Problems In order to achieve the above object, the present inventors have conducted intensive studies, and as a result, have found that proanthocyanidins significantly suppress muscle atrophy. Thus, the present invention has been completed. That is, the first invention is a muscle atrophy inhibitor comprising proanthocyanidin as an active ingredient. The second invention is the muscular atrophy inhibitor according to the above, wherein the proanthocyanidin is an extract obtained from juice of grape seeds, pericarp, or fruits.

[0006]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail. In addition, the muscular atrophy inhibitor described in the present invention refers to a substance having an action of preventing or delaying muscle atrophy and / or alleviating symptoms. First, the proanthocyanidins used in the present invention include grapes, persimmons, apples, pine, soybeans, soybeans, vegetable-derived ones such as red beans, and wines, fruit wines, fruit juices, and other food-derived ones. There are no restrictions on the origin of the product or on the use of raw materials, the production method, and the purification method. Among them, grape seeds are suitable as a raw material because high content proanthocyanidins can be easily obtained by extraction because of high content or low content of impurities such as sugars. As a method of obtaining proanthocyanidins by a method other than extraction from grape seeds,
Extract from red beans [Ariga et al., Agricultural Biological Chemistry (Agric. Biol. Chem.) Vol. 45,
2709-2712, 1981), extraction from pine bark (RWHem
ingway et al., Phytochemistry No. 22
Volume, pp. 275-281, 1983), separation from apple cider (AG
H.Lea et al., Journal of Science of Food Agriculture (J.Sci. Food Agric.) Vol. 29, 4
71-477, 1978].

The proanthocyanidins obtained as described above can be obtained in liquid or semi-solid form.
If the extraction solvent is removed therefrom by a known method such as distillation under reduced pressure, spray drying, freeze drying and the like, it can be used as a proanthocyanidin-containing concentrate or dried product as it is. As shown in the examples of JP-A-11-80148, a proanthocyanidin composition having a high purity of 90% or more can be easily obtained by using optimal raw materials and extraction conditions. Products containing proanthocyanidins as a main component are also commercially available. For example, “KPA”, “Gravinol”, “Gravinol Super” using grape seeds as a raw material [Kikkoman Co., Ltd.] "Applephenone" made from unripe apples (Nikka Pesky Co., Ltd.) and "Pycnogenol" made from bark of shore pine [Hofar Research Co., Ltd. (Switzerland)]. is there. In the case of oral ingestion, the amount of proanthocyanidin may be, for example, 20-1000 mg, preferably 30
About -400mg. The amount of vitamin intake when combined with vitamins is about 20-1000 mg, preferably about 30-400 mg per day for adults.

[0008]

According to the present invention, by taking proanthocyanidin, muscle atrophy can be remarkably suppressed as shown in the experimental examples, and the gospel is brought to people suffering from muscle atrophy.

[0009]

[Experimental example] An experimental example is shown below.

[Experimental Example 1] Inhibitory effect of gravinol super (GVS) on hind limb muscle atrophy in rats (effect comparison test between GVS and vitamin E) Kondo et al.'S method (Kondo H., Acta. Physiol. Sscand. , 14
2, 527-528 (1991)), a rat hind limb muscle atrophy model was prepared. That is, a 13-week-old Crj: Wistar rat (♂, 6 rats / group × 3 groups, 18 rats) was inserted with a Kirschner steel wire in the right hind leg ankle joint to immobilize the right hind leg muscle and immobilize. The left hind limb joint was free to move without being fixed. 19 days before the treatment and 12 days after the treatment
During the day, six animals were allowed to freely take only the normal feed (control group). Another 6 animals were allowed to freely take the feed obtained by adding GVS to the normal feed at a rate of 1% (GVS group). Six animals were given an intraperitoneal injection of vitamin E (α-tocopherol) daily at 30 mg / kg body weight / day × 19 days while receiving a normal diet (vitamin E group). At the end of the test, sample the soleus muscles of both hind limbs and measure the wet weight, and calculate the atrophy rate from the difference between the wet weight of the right hind limb soleus muscle (non-solid side) and the wet weight of the right hind limb soleus muscle (non-solid side) in each group. did. Table 1 shows the results of the average atrophy rate of the soleus muscle of the right limb of each group of rats. The value of the atrophy rate was smaller in the GVS group than in the control group, and the effect of GVS on inhibiting muscle atrophy was confirmed (Table 1). On the other hand, vitamin E had no effect (Table 1). Table 1 Test group Average atrophy rate of rat soleus soleus muscle (%) Control group 26.47% GVS group 13.22% Vitamin E group 36.36%

[0010]

[Experimental Example 2] Effect of Gravinol Super (GVS) on hind limb muscle atrophy in rats (GVS, applephenone,
Comparative test results with vitamin E and vitamin C) 13-week-old Crj: Wistar rats (♂, 6 rats / group × 5 groups, 30 rats)
And a rat hind limb muscular atrophy model was prepared in the same manner as in Experimental Example 1 described above. The test group consisted of a control group in which the normal feed was freely ingested for 19 days from 7 days before the treatment to the 12th day of the treatment, and a GVS in which the normal diet was supplemented with a diet in which GVS was added at a ratio of 1%. Apple phenone group fed with apple phenone (produced by Nikka Whiskey Co., Ltd., proanthocyanidins made from unripe apples, total flavanol content: 90% or more) to normal feed at a rate of 1% Vitamin freely fed on diet containing 1% tocopherol acetate
Group E and a group of vitamin C in which a normal diet to which vitamin C was added at a ratio of 1% was freely ingested. At the end of the test, as in Experimental Example 1 above, the wet weight of the soleus muscle of the right hind limb (fixed side) relative to the wet weight of the soleus muscle of the left hind limb (non-fixed side) of each group.
The atrophy rate was calculated from the difference. Table 2 shows the results of the average atrophy rate of the soleus muscle of the right hind limb of each group of rats. GVS and vitamin C were confirmed to have muscle atrophy inhibitory effects, but the effects were:
GVS was stronger than Vitamin C (Table 2). On the other hand, applephenone and vitamin E had no effect (Table 2). Table 2 Test group Average atrophy rate (%) of rat soleus soleus muscle Control group 23.02% GVS group 19.62% Applephenone group 23.95% Vitamin E group 25.33% Vitamin C group 20.74%

[0011]

The present invention will be described more specifically below with reference to examples. Example Production Example 1 (oral medicine: tablet) Grape seed extract [Gravinol Super, manufactured by Kikkoman Corporation] 100 mg Lactose 100 mg Starch 17 mg Magnesium stearate 3 mg One tablet or more is made by a conventional method. Tablets were obtained to give tablets.

 ──────────────────────────────────────────────────続 き Continued on the front page F term (reference) 4C062 FF56 4C086 AA01 AA02 BA08 MA01 NA14 ZA23 ZA94 4C088 AB56 AC04 NA14 ZA23 ZA94

Claims (2)

[Claims] A muscular atrophy inhibitor comprising proanthocyanidin as an active ingredient. 2. The proanthocyanidin is grape seed,
The muscular atrophy inhibitor according to claim 1, which is an extract obtained from a squeezed liquid of a pericarp or a fruit.