JP2002322081A - Inhibitor of genetic damage in living body - Google Patents

Abstract

(57) [Summary] [Object] An object of the present invention is to provide an agent for suppressing gene damage in a living body, characterized by containing one or more extracts selected from mushrooms belonging to the genus Amanita. The present invention is an agent for suppressing gene damage in a living body, comprising an extract selected from one or more of mushrooms belonging to the genus Amanita. The agent for inhibiting genetic damage of a living body containing one or two or more extracts selected from mushrooms belonging to the genus Mushroom of the present invention is excellent in stability and has an excellent inhibitory effect on genetic damage of a living body.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to the suppression of genetic damage in living organisms, and more particularly, to the suppression of genetic damage caused by mutagenic substances such as ultraviolet rays when incorporated in foods, cosmetics, quasi-drugs or pharmaceuticals. The present invention relates to a gene damage inhibitor exhibiting its effectiveness in aging such as skin.

[0002]

2. Description of the Related Art Mutagens are present in the environment such as food, air and water, and various organs of living organisms are constantly at risk of genetic damage. Although living organisms have genes and enzymes that repair these damages as they are born, not all damages are repaired, so the accumulation of genetic damages is thought to be the cause of increased carcinogenesis and accelerated aging. Have been. To reduce these risks,
Foods, cosmetics, quasi-drugs, pharmaceuticals and the like containing a carcinogenesis inhibitor have been developed. On the other hand, among various organs, the skin is located in the outermost layer of a living body and is the organ in which a gene is most easily damaged by the influence of ultraviolet rays or the like. Due to their susceptibility, increasing the amount of UV exposure of living organisms due to the recent destruction of the ozone layer is a serious problem. In order to address this problem, it is common to use ultraviolet absorbers or reflectors in cosmetics, quasi-drugs and the like.

[0003]

Although research on carcinogenesis inhibitors is progressing worldwide, it is expected that foods, cosmetics, quasi-drugs or pharmaceuticals containing a further gene damage inhibitor will be developed. In addition, the protective action of the ultraviolet absorber or the reflective agent on the skin is not perfect due to uneven coating, sweating, etc., and there is a need for a biological gene damage inhibitor that complements the action of repairing genes and the like.

[0004]

[Means for Solving the Problems] Under such circumstances,
The present inventors have conducted intensive studies to obtain an inhibitor centered on genetic damage in a living body due to ultraviolet rays. As a result, an extract selected from one or more mushrooms belonging to the genus Ganoderma has excellent gene damage suppression. The present inventors have found that they exhibit an agent action and completed the present invention.

[0005] The agent for suppressing gene damage to a living body used in the present invention is intended to suppress gene damage to cells of the living body including the skin as described above. More specifically, it means preventing carcinogenesis and aging by suppressing gene damage caused when a living body is irradiated with ultraviolet rays.

[0006] That is, the present invention is an agent for suppressing gene damage to a living body selected from one or more of mushrooms belonging to the genus Ganoderma.

The genus Ganoderma is a basidiomycete (Basid)
iomycetes), Aphillium (Aphyll)
ophorales), Polychondrate (Polyp)
oraceae), Ganoderm
eae), and include, for example, ginseng mushroom, maggot beetle, ginseng ginseng, sugman ginseng, giant ginseng, bat ginseng, ginseng ginseng and the like. In addition, as Ganoderma lucidum, Reishi (red turf), black Reishi (black turf), purple turf, blue turf, yellow turf, white turf, and the like are described in Shinnohon Soukei. These scientific names can be found in the literature [Acta
Microbiological Sinica 19
(3), 265-279, 1979], are classified into, but not limited to, Reishi (scientific name: Ganoderma lucidum), Black Reishi (scientific name: Ganoderma atrum), and Shishiba (scientific name: Ganoderma sinense). Not something.

The black turf (black turf) used in the present invention grows on trees of the maple family mainly in southern China (south of Shanghai). In terms of efficacy, according to Shinnobu Sokyo, Black Reishi is
Treats symptoms of urinary retention and difficulty urinating, lower stomach bloating,
It is said to promote urination and enhance kidney function.

[0009] Reishi (red turf) used in the present invention grows widely on broadleaf trees. In terms of efficacy, according to Shinnobu Sokyo, Reishi (Akashiba) mainly treats thoracic chest (sickness with heartache and lump), strengthens the function of the heart, supplements vitality,
It is stated to increase thinking and prevent forgetfulness.

[0010] The maggot (scientific name: Ganoderma neojaponicum) used in the present invention
Is distinguished in classification from Mannentake.

[0011] Among the mushrooms belonging to the genus Mannentake, Mannentake and maggot are preferable.

The extract of mushrooms belonging to the genus Amanita used in the present invention is obtained by extracting from the fruiting bodies of mushrooms belonging to the genus Amanita. The preparation method is not particularly limited, and may be, for example, one extracted by heating or one extracted at ordinary temperature. Also,
Some of the above extracts are commercially available, and these can be used.

Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (propylene glycol, glycerin, , 3-butylene glycol, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.),
Ethers (ethyl ether, propyl ether, tetrahydrofuran, etc.); Preferred are water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, propylene glycol and 1,3-butylene glycol. These solvents may be used alone or in combination of two or more.

The extract of mushrooms belonging to the genus Amanita may be used as it is in the form of an extracted solution. If necessary, the extract may be subjected to treatments such as concentration, dilution and filtration, and decolorization and deodorization treatment with activated carbon or the like. May be. Further, the extracted solution may be subjected to a treatment such as concentration and drying, spray drying, and freeze drying to be used as a dried product.

The above-mentioned extract may be used as it is as the agent for suppressing gene damage to the living body of the present invention, and ordinary foods, cosmetics and pharmaceuticals may be used as long as the effect of the extract of mushroom belonging to the genus Amanita is not impaired. Quasi-drugs, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, humectants, powders used in pharmaceutical products Components such as a body, an ultraviolet absorber, a thickener, a pigment, an antioxidant, a whitening agent, a chelating agent, an excipient, a stabilizer, a preservative, a binder, a disintegrant and the like can also be blended.

[0016] The agent for suppressing genetic damage of a living body according to the present invention is a food,
It can be used for cosmetics, quasi-drugs and pharmaceuticals, and as the dosage form, for example, tablets, beverages, lotions, creams, emulsions, gels, aerosols, ointments,
Cataplasms, pastes, plasters, essences, packs, detergents, baths, foundations, powders, lipsticks, powders, pills, tablets, injections, suppositories, emulsions, capsules, granules, liquids (tinctures) Fluid extract, alcoholic beverage, alcohol, suspension, limonade, etc.).

The amount of the extract of mushroom belonging to the genus Ganoderma used in the present invention is 0.1% in terms of dry matter with respect to the total amount of the food, cosmetics, quasi-drug or pharmaceutical of the present invention.
0001% by weight or more is preferable, and 0.0005 to
10% by weight is good. If the amount is less than 0.0001% by weight, a sufficient effect is hardly exhibited. If it is added in an amount exceeding 10% by weight, the effect is not greatly enhanced and is uneconomical. The method of addition may be added in advance or may be added during production, and may be appropriately selected in consideration of workability.

[0018]

EXAMPLES In order to explain the present invention in detail, examples of the production of the extract used in the present invention, prescription examples of the present invention and experimental examples will be given below, but the present invention is not limited to these examples. Absent. The term “parts by weight” in Examples means “parts by weight”, and “%” means “% by weight”.

Production Example 1 Hot water extract of Kuroreshiba 400 mL of purified water was added to 20 g of dried Kuroreshiba,
After extraction at 5-100 ° C. for 2 hours, the mixture was filtered, and the filtrate was concentrated and freeze-dried to obtain 1.3 g of a hot water extract of Reishi black.
Obtained.

Production Example 2 Hot water extract of Reishi (Akashiba) 400 mL of purified water was added to 20 g of dried Reishi (Akashiba), extracted at 95-100 ° C for 2 hours, filtered, and the filtrate was filtered. Was concentrated and freeze-dried to obtain 1.4 g of hot water extract of Reishi (Akashiba).

Production Example 3 Hot water extract of maggots 400 mg of purified water was added to 20 g of dried maggots, extracted at 95-100 ° C for 2 hours, filtered, the filtrate was concentrated and freeze-dried. 2.0 g of hot water extract of maggot was obtained.

Production Example 4 Ethanol Extract of Kuroreshiba 900 mL of ethanol was added to 100 g of the dried product of Kuroreshiba, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness. 1.5 g of turf ethanol extract was obtained.

Production Example 5 Ethanol extract of Reishi (Akashiba) 900 mL of ethanol was added to 100 g of dried Reishi (Akashiba), extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness. Thus, 1.6 g of an ethanol extract of Reishi (Akashiba) was obtained.

Production Example 6 Ethanol extract of maggots To 900 g of ethanol was added to 100 g of dried maggots, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain an ethanol extract of maggots. 1.5 g were obtained.

Production Example 7 Extract of aqueous solution of black reishi from 1,3-butylene glycol 1 kg of purified water and 1 kg per 100 g of dried black reishi
Was added and extracted at room temperature for 7 days, followed by filtration to obtain 1.9 kg of an aqueous solution of 1,3-butylene glycol of Kuro Reishi.

Production Example 8 1,3-butylene glycol aqueous solution extract of Reishi (Akashiba) 1 kg of purified water and 1 kg of 1,3-butyleneglycol were added to 100 g of dried Reishi (Akashiba) at room temperature. After extraction for 7 days, the mixture was filtered to obtain 1.9 kg of a 1,3-butylene glycol aqueous solution extract of Reishi (Akashiba).

Production Example 9 Extract of aqueous solution of maggots in 1,3-butylene glycol To 100 g of dried maggots was added 1 kg of purified water and 1 kg of 1,3-butylene glycol, extracted at room temperature for 7 days, and filtered. 1.9 kg of a 1,3-butylene glycol aqueous solution extract of maggots was obtained.

Example 1 Cream 1 Formulation Formulation Amount 1. Hot water extract of Black Reishi (Preparation Example 1) 0.05 part Squalane 5.5 3. Olive oil 3.0 4. Stearic acid 2.05. Beeswax 2.0 6. Octyldodecyl myristate 3.5 7. 7. Polyoxyethylene cetyl ether (20EO) 3.0 Behenyl alcohol 1.5 9. Glycerin monostearate 2.5 10. Fragrance 0.1 11.1,1,3-butylene glycol 8.5 12. 12. ethyl paraoxybenzoate 0.05 13. Methyl paraoxybenzoate 0.2 Make the total amount 100 with purified water [Production method] Heat and dissolve components 2 to 9 and mix at 70 ° C.
Oil phase. Components 1 and 11 to 14 are dissolved by heating and mixed, and the mixture is kept at 75 ° C. to form an aqueous phase. The oil phase is added to the water phase, emulsified, cooled while stirring, and the component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

Example 2 Cream 2 In Example 1, the hot water extract of Black Reishi was used for Reishi (Akashiba).
The hot water extract (Production Example 2) was replaced with Cream 2.

Example 3 Cream 3 Cream 3 was prepared in the same manner as in Example 1 except that the hot water extract of Black Reishi was replaced with a hot water extract of Magnolia (Preparation Example 3).

Comparative Example 1 Conventional Cream A conventional cream was prepared by replacing the hot water extract of Kuroishiba with purified water in Example 1.

Example 4 Lotion Formulation Formulation Amount 2. Ethanol extract of Black Reishi (Preparation Example 4) 0.1 part 2.1,3-butylene glycol 8.0 Glycerin 2.0 4. Xanthan gum 0.02 5. Citric acid 0.01 6. Sodium citrate 0.17. Ethanol 5.0 8. 8. Methyl paraoxybenzoate 0.19. Polyoxyethylene hydrogenated castor oil (40EO) 0.110. Perfume appropriate amount [Production method] Components 1 to 6 and 11 and components 7 to 10 are each dissolved uniformly, and the both are mixed and filtered to obtain a product.

Example 5 Emulsion Formulation Formulation Amount 1. Ethanol extract of Reishi (Akashiba) (Production Example 5) 0.5 part Squalane 5.0 3. Olive oil 5.0 4. Jojoba oil 5.0 5. Cetanol 1.5 6. Glycerin monostearate 2.07. 7. Polyoxyethylene cetyl ether (20EO) 3.0 8. Polyoxyethylene sorbitan monooleate 2.0 (20EO) Perfume 0.1 10. Propylene glycol 1.0 11. Glycerin 2.0 12. 12. Methyl paraoxybenzoate 0.2 Make the total amount 100 with purified water [Production method] Heat and dissolve components 2 to 8 and mix at 70 ° C.
Oil phase. Components 1 and 10 to 13 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The oil phase is added to the water phase and emulsified, cooled while stirring, and the component 9 is added at 45 ° C, and further cooled to 30 ° C to obtain a product.

Example 6 Gel Formulation Formulation Amount 1. Ethanol extract of Magnolia (Preparation Example 6) 0.1 part Ethanol 5.0 3. Methyl paraoxybenzoate 0.14. Polyoxyethylene hydrogenated castor oil (60EO) 0.15. Fragrance appropriate amount 6.1,3-butylene glycol 5.0 7.0 Glycerin 5.0 8. Xanthan gum 0.19. Carboxyvinyl polymer 0.2 10. Potassium hydroxide 0.2 11. [Production method] Components 1 to 5 and components 6 to 11 are each dissolved uniformly, and both are mixed to obtain a product.

Example 7 Ointment Formulation Formulation Amount 1. Hot water extract of Black Reishi (Preparation Example 1) 2.0 parts 2. Polyoxyethylene cetyl ether (30 EO) 2.0 Glycerin monostearate 10.0 4. Liquid paraffin 5.0 5. Cetanol 6.0 6. Methyl paraoxybenzoate 0.17. Propylene glycol 10.0 8. Make the total amount 100 with purified water [Production method] Heat and dissolve components 2 to 5 and mix at 70 ° C.
Oil phase. Components 1 and 6 to 8 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The oil phase is added to the water phase, emulsified, and cooled to 30 ° C. with stirring to obtain a product.

Example 8 Pack Formulation Compounding Amount 0.2 parts of 1,3-butylene glycol aqueous solution extract of Kuroreshiba (Production Example 7) Polyvinyl alcohol 12.0 3. Ethanol 5.0 4.1 1,3-butylene glycol 8.0 5.0. Methyl paraoxybenzoate 0.26. Polyoxyethylene hydrogenated castor oil (20EO) 0.5 7. Citric acid 0.18. Sodium citrate 0.3 9. Appropriate amount of fragrance 10. [Production method] Components 1 to 10 are uniformly dissolved to give a product.

Example 9 Foundation Formulation Compounding Amount 1.0 part of 1,3-butylene glycol aqueous solution extract of Reishi (Akashiba) (Production Example 8) Stearic acid 2.4 3. 3. Polyoxyethylene sorbitan monostearate 1.0 (20 EO) Polyoxyethylene cetyl ether (20EO) 2.05. Cetanol 1.0 6. Liquid lanolin 2.0 7. Liquid paraffin 3.0 8. 8. Isopropyl myristate 6.5 Butyl paraoxybenzoate 0.110. Sodium carboxymethylcellulose 0.1 11. Bentonite 0.5 12. Propylene glycol 4.0 13. Triethanolamine 1.1 14. 14. Methyl paraoxybenzoate 0.2 Titanium dioxide 8.0 16. Talc 4.0 17. Bengala 1.0 18. Iron oxide yellow 2.0 2.0 19. Perfume appropriate amount 20. The total amount is made 100 with purified water. [Production method] Components 2 to 9 are heated and dissolved, and kept at 80 ° C to obtain an oil phase. Swell component 10 well with component 20, then add components 1 and 11-14 and mix uniformly.
Components 15 to 18 crushed and mixed by a crusher are added to this,
Stir with a homomixer and maintain at 75 ° C to obtain an aqueous phase. The oil phase is added to the aqueous phase while stirring, cooled, and the component 19 is added at 45 ° C, and cooled to 30 ° C with stirring to obtain a product.

Example 10 Bath Agent Formulation Compounding Amount 1. Aqueous 1,3-butylene glycol aqueous extract of maggots 1 part (Production Example 9) Sodium bicarbonate 50 3. Yellow No. 202 (1) Suitable amount 4. Appropriate amount of fragrance 5. The total amount is made 100 with anhydrous sodium sulfate.

Example 11 Tablet Confectionery Formulation Amount 1. 1. Hot water extract of Black Reishi (Preparation Example 1) 2 parts 2. dried corn starch 50 Erythritol 40 4. Citric acid 5 5. Sucrose fatty acid ester 3 6. Appropriate amount of fragrance 7. [Production method] Components 1 to 4 and 7 are mixed and granulated. Ingredients 5 and 6 are added to the formed granules and tableted.
One grain is 1.0 g.

Example 12 Beverage Formulation Blended Amount 1. Hot water extract of Reishi (Akashiba) (Production Example 2) 1.0 part Stevia 0.05 3. Malic acid 5.0 4. Perfume 0.15. The total amount is adjusted to 100 with water. [Production method] Components 2 and 3 are dissolved in a part of the component 5. Then, ingredients 1, 4 and 5 are added and mixed.

Example 13 Tablet Formulation Compounding Amount 1. Hot water extract of maggots (Production Example 3) 10 parts Corn starch 10 3. Purified sucrose 20 4. Carboxymethylcellulose 10 5. Microcrystalline cellulose 35 6. Polyvinylpyrrolidone 5 7 Talc 10 [Production method] Components 1 to 5 were mixed, and then an aqueous solution of component 6 was added as a binder and granulated by a conventional method. After adding component 7 as a lubricant to this and blending, 1 tablet 100 m
g tablets.

Example 14 Powder Formulation Formulation Amount 1. Hot water extract of Black Reishi (Production Example 1) 10 parts Corn starch 40 3. Microcrystalline cellulose 50 [Production method] The above components were mixed and used as a powder in a conventional manner.

Example 15 Injection Formulation Formulation Amount 1. Hot water extract of Black Reishi (Preparation Example 1) 1.0 part 2. Polyoxyethylene (60) hydrogenated castor oil 3.7 Sesame oil 0.24. Sodium chloride 0.95. Propylene glycol 4.0 6. Phosphate buffer (0.1 M, pH 6.0) 10.0 7. Make the total amount 100 with distilled water. [Production method] Half of the components 1, 2, 3 and the component 5 are mixed and heated and dissolved at about 80 ° C, and half of the components 4, 6 and the component 5 are added thereto. The previously dissolved component 7 was heated to about 80 ° C., and the total amount was made 1,000 ml. This aqueous solution was dispensed into 1 ml ampules, sealed, and then heat-sterilized.

Next, experimental examples will be described in order to explain the effects of the present invention in detail.

Experimental Example 1 Salmonella typhimurium, a histidine-requiring mutant commonly used for mutation detection
In 0.1 ml of the bacterial solution at the end of logarithmic growth of TA100,
0.1 ml of a 50 mg / ml solution of the sample of the present invention (Preparation Examples 1 to 3) and 0.1 ml of a phosphate buffer (pH 7.4).
5 ml at the same time, UVA using Toshiba black lamp
1.2 J / cm ² and UVB 0.15 J / c
m ² was irradiated. After irradiation, 2 ml of top agar containing histidine-biotin was added and poured onto a minimal glucose agar plate. After solidification, the cells were cultured at 37 ° C. for 72 hours, and the number of generated colonies was counted. A non-irradiated group was also provided as a control. In addition, the test was also performed on a post-treatment group in which the sample was not added during the ultraviolet irradiation, and was added to the top agar after the irradiation.
Furthermore, to determine the number of viable bacteria, the respective processing solutions were diluted 10 ⁶ fold with physiological saline, using instead the agar solution was added to nutrient broth as the top agar, are working in the same manner the following Was.

Table 1 shows the ratio of the number of colonies on the plate to which the sample was added and cultured, with distilled water as 100.
In the simultaneous treatment of Production Examples 1 to 3, an extremely strong inhibitory effect on the induction of mutation caused by ultraviolet light was observed. The post-treatment was weaker than the simultaneous treatment, but showed a strong suppression result. The number of colonies in the non-irradiated group was the same as that in distilled water. The number of viable bacteria did not decrease.

[0047]

[Table 1]

The same examination was carried out for Production Examples 4 to 9, and the effect of suppressing mutation was recognized. As described above, Experimental Example 1 revealed that the extract of black reishi, reishi (red turf) and maggot was highly effective in suppressing gene damage. Since this inhibitory effect was observed even in post-treatment, it was considered that it also had a gene repairing effect.

EXPERIMENTAL EXAMPLE 2 Inhibitory Effect of Sunburn Cell Formation A sunburn cell is a cell in which cytoplasm, which is recognized as a characteristic finding when the epidermis after sunburn is observed histopathologically, is eosinophilic and strongly stained, and the nucleus is concentrated. . This is a finding indicating the necrosis of epidermal cells, and its formation is known to involve genetic damage that is affected by ultraviolet light. Therefore, the effect of the cream of the present invention (Examples 1 to 3) on the formation of sunburn cells was examined. That is, the cream of the present invention (Examples 1 to 3) and the conventional cream (Comparative Example 1) were applied to the outer skin of the pinna of an ICR mouse (male, 6 weeks of age) for 5 m.
g / cm ² . 24 hours later Toshiba FL as a light source
Using a 20SE lamp, under Nembutal anesthesia, 300
The outer skin of the mouse pinna was irradiated with UVB of mJ / cm ² . At this time, the cream applied 24 hours before was removed immediately before irradiation. Twenty-four hours after UVB irradiation, auricular skin tissues were collected, fixed in formalin, cut into thin sections according to a standard method, and stained with hematoxylin-eosin. Each tissue specimen was observed under a microscope (magnification: 300), and the average of the number of sunburn cells in any 10 visual fields was defined as the number of sunburn cells of the specimen. The sunburn cell formation inhibition rate of each sample was determined by comparison with the number of sunburn cells of control mice to which no cream was applied.

Table 2 shows the results of these tests. UV by applying the cream of the present invention (Examples 1-3)
The suppression of sunburn cell formation by B irradiation was recognized. On the other hand, when the conventional cream (Comparative Example 1) was applied, a sunburn cell was formed as in the case where the cream was not applied. In addition, the cream of the present invention (Examples 1 to 3) was heated at 40 ° C.
After storing for 1 week at, a similar test was conducted.
A similar inhibitory effect on sunburn cell formation was observed.

[0051]

[Table 2]

The same tests were performed on Examples 4 to 10, and a similar effect of suppressing the formation of sunburn cells was observed.

[0053]

As described above, the agent for suppressing genetic damage of a living body containing one or more extracts selected from the mushrooms belonging to the genus Ganoderma of the present invention has excellent stability and excellent action. Was. In addition, foods, cosmetics, quasi-drugs, or pharmaceuticals containing these compounds also exhibited an excellent inhibitory effect on gene damage in living organisms.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61P 17/00 A61P 17/00 43/00 43/00 (72) Inventor Tomonori Katata 2 Tomicho, Nishi-ku, Nagoya-shi -7 F-term in Menard Cosmetics Co., Ltd. F-term (reference) 4B018 LB01 LB08 MD84 ME10 4C083 AA082 AA111 AA112 AA122 AB032 AB052 AB232 AB242 AB312 AB352 AB432 AB442 AC022 AC072 AC102 AC122 AC182 AC242 AC302 AC352 AC422 AC432 AC442 AC482 AC542 AC542 AD352 AD512 BB51 CC03 CC04 CC05 CC07 DD23 DD31 DD41 EE07 EE12 4C088 AA06 AC17 BA08 BA09 BA10 BA37 CA05 CA06 CA08 CA11 CA17 MA07 MA17 MA21 MA22 MA28 MA35 MA43 MA52 MA66 NA14 ZA89 ZC21

Claims (2)

[Claims] 1. An agent for suppressing genetic damage to a living body, comprising an extract selected from one or more of mushrooms belonging to the genus Amanita. 2. A food, cosmetic, quasi-drug or drug containing the agent for suppressing genetic damage of a living body according to claim 1.