JP2002212100A - Sphingolipid composition for acne prevention and treatment - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for suppressing harmful microorganisms to the skin such as Propionibacterium acnes, eliminating skin inflammation due to acne, recovering damaged skin quickly, and treating neatly without leaving a wound. Provided is a pharmaceutical composition for treating acne. SOLUTION: The pharmaceutical composition for preventing and treating acne is obtained by mixing 0.1 to 10.0 parts by weight of sphingolipid, which is a main component of the skin protective film, with 1.0 part by weight of a chicken egg antibody against Propionibacterium acnes.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD [0001] The present invention relates to Propionibacterium acnes (Pr
acnes), a chicken egg antibody containing sphingolipids and an acne treatment containing sphingolipids, which have an excellent function of recovering skin damage due to acne while suppressing harmful microorganisms and skin inflammation of the skin and treatment of acne containing sphingolipids; The present invention relates to a pharmaceutical composition for prevention.

[0002]

BACKGROUND OF THE INVENTION Acne is a chronic inflammatory disease of the sebaceous glands on the faces of adolescent men and women, especially the cheeks and forehead, which is characterized by comedones, papules, pustules, cysts, nodules and sometimes scarring. It is one of the related diseases (Pilosabaceous Disease). Acne mainly occurs in puberty and declines in the mid-20s, and appears mainly in more severe forms in boys. Comedo, which is the underlying disease of acne, is produced by stagnation of keratin and sebum due to hyperkeratosis of the hair follicle epithelium, and there are open and closed comedones. Acne is mainly acnecomedo, acne papulosa, and sclerotic acne (acne indurat) depending on the type of rash that appears.
a) etc.

[0003] The cause of acne has not been elucidated exactly yet, but the main causes are excessive secretion of sebum (Highsebum excretion rate), cornification of the duct (Ductal Hyper cornification), Pilose glands (Pilose
Unusual microbial growth and inflammatory response in baceous ducts) are known as the four major causes. In general, it is caused by the combined action of each of these factors, etc., and as a result of the acne-causing bacterium infecting and multiplying the innate diathesis and excessive secretion of sebum by the androgen hormone,
It is considered as a complex phenomenon that causes inflammation and, in severe cases, scarring.

[0004] Propionibacterium acnes (Propionibacteriu) is a microorganism that exacerbates the acne phenomenon.
macnes) is known to be a typical one, but other than that, Propionibacte
riumavidum), Staphylococcus epidermis (Sta
It is known that phylococcus epidermis) and mallaceria furfur (Malasseria furfur) act in combination to worsen symptoms.

[0005] Propionibacterium acnes is known to be deeply involved in pre-onset comedogenesis and inflammation in acne. If the shaft of the funnel becomes keratinized and closed, it causes sebum excretion disorders and increases the number of propionibacteria, while neutralizing the fatty acids in the sebum due to the action of lipase secreted by these bacteria. Decomposed to produce free fatty acids. The free fatty acids and the like have an effect of inducing sticky keratin growth and generating comedones. Propionibacterium acnes proliferates in comedones and produces neutrophil chemotactic factors and various enzymes, and if these leak from the comedones to the dermis, inflammation is rejuvenated.

[0006] At present, methods for preventing and treating acne include:
There are methods such as suppression of sebum hypersecretion, removal of hair follicle closure, and inhibition of bacterial growth, but none of these methods have been satisfactory. As a treatment for acne, there is a female hormone used as a sebum secretion inhibitor, because it inhibits the growth of the epidermis and suppresses the action of the sebaceous glands and affects other hormone balances in the living body. , Rarely used.

[0007] Erythromycin and the like, which have an excellent inhibitory effect on Propionibacterium acnes, are used. In addition, since such antibiotics have no specificity for bacteria, they affect the entire resident bacteria and induce the development of bacteria resistant to antibiotics (The Lance
t, vol. 350, 972-973, 1997), often making acne treatment more difficult. Furthermore, since it kills all of the skin-resident microorganisms, it is still more difficult to use it not only for therapeutic purposes but also for preventive purposes.

Accordingly, the present invention uses a chicken egg antibody against Propionibacterium acnes to effectively control acne-causing bacteria with specificity to the bacterium and little effect on other skin-resident bacteria. The aim is to develop an improved therapeutic agent. However, even if chicken eggs antibody is used to effectively suppress Propionibacterium acnes, acne exacerbation by other microorganisms,
It has been difficult to effectively treat skin inflammation and the resulting skin damage.

In general, it has been observed that the acne site has a remarkably reduced ceramide content in the stratum corneum (Stratumcorneum), which is the skin protective film, as compared with the normal site.
In the case of an acne site, the ceramide content is about 25 to 30%, while the normal site has an average of 45%. (Arc
hDermatol Res, 287: 214-218 (1995)). In addition, the content of sphingosine and phytosphingosine is lower than that in a normal site, providing favorable conditions for the growth of microorganisms.

[0010] In addition, when ceramide and sphingosine derivatives become deficient, they provide good conditions for microorganisms, and at the same time, promote the occurrence of skin inflammation. Results in

[0011] Phytosphingosin
e) is a kind of sphingolipid and is a physiologically active substance having various biological functions as a signal transmitting substance in a signal transmitting system as well as a structural function as one of major cell membrane components of a living body. (Okazaki et al., 1989; K
im et al., 1991).

Sphingolipids have been shown to play a key role in the current state of life such as regulation of cell growth, proliferation, differentiation and induction of apoptosis (Hannun, 1994, 1996;
Hannunand Obeid, 1995) TNF-α, IL-1, γ-IFN
And FAS ligands. Sphingolipids occupy the majority of skin protective membrane lipids and maintain the skin moisturizing and regenerate the skin protective membrane, and at the same time play the role of the primary defense membrane of the skin against attack from microorganisms.

In addition, sphingolipids inhibit and activate protein kinase C, regulate EGF receptor activity, and mobilize intracellular calcium ions (Mobilizati).
on) Induction, regulation of Rb, NF-B gene expression, etc., and is widely used in anti-inflammatory and anti-cancer research. In recent years, most skin diseases including acne and atopic dermatitis are caused by a decrease in the content of various sphingolipids in skin lipids (Arch. Dermatol. Res. 289).
(1997)), it is possible that sphingolipids could be used as a next-generation drug material that can replace steroid hormones, which are anti-inflammatory drugs and anti-inflammatory drugs, as the main raw materials for the treatment of skin diseases. Expected to be very large.

[0014] Phytosphingosine and ceramides 3 and 4 having phytosphingosine as the basic skeleton have excellent skin moisturizing functions and regenerate damaged skin protective films, and are used worldwide for skin care cosmetics. The range has been extended to hair products. Phytosphingosine has begun to be used as a functional ingredient such as a humectant and an anti-aging agent in the cosmetics industry in combination with ceramide, and its skin regeneration and antibacterial and anti-inflammatory effects are much higher than ceramide, and its use is rapidly increasing.

[0015] In the pharmaceutical industry, studies on atopic dermatitis, acne, wound treatments and the like have already been conducted, and phytosphingosine will be used as an important material for functional cosmetics in the future. Its use is expected to expand to products, products for regenerating damaged skin, etc. and remedies for atopic dermatitis, remedies for acne, remedies for dry habit and skin itch, remedies for wounds, and the like.

On the other hand, with respect to the prevention and treatment of acne using a chicken egg antibody, JP-A-1-313439, JP-A-2-21919
No. 08, JP-A-2-121909, JP-A-2-121910, etc., but this is the case where only chicken egg antibodies are used, and as in the present invention, the protozoa of the causative bacteria of acne. No remedy for acne using a specific chicken egg antibody against Pionibacterium acnes in combination with a sphingolipid such as phytosphingosine which is effective in suppressing skin inflammation and recovering a damaged skin protective film has not yet been developed.

[0017]

Accordingly, the present invention provides
Propionibacterium acnes and other methods of controlling harmful microorganisms to the skin to eliminate acne-induced skin inflammation, as well as a method for recovering damaged skin quickly and treating neatly without leaving scars, and a pharmaceutical composition for treating acne The purpose is to provide.

[0018]

Means for Solving the Problems As a result of intensive studies to achieve the above object, the present inventors have found that a sphingolipid and a specific antibody against Propionibacterium acnes, the causative bacterium of acne, are simultaneously obtained. The inventor has found that the composition contained can suppress skin inflammation, recover damaged skin quickly, and treat neatly without leaving any scars. The inventors have completed the present invention based on these findings.

That is, according to the present invention, sphingolipid 0.1 to 10.0, which is a main component of the skin protective membrane, per 1.0 part by weight of hen's egg antibody to acne bacteria Propionibacterium acnes
A pharmaceutical composition for preventing and treating acne, characterized by mixing parts by weight.

In the above invention, the chicken egg antibody is preferably γ-libetin contained in a fraction having a molecular weight of 100,000 or more when a water-soluble protein portion is fractionated by a membrane filter.

In the above invention, the sphingolipid is ceramide 3, ceramide 4, phytosphingosine,
It is preferably at least one selected from N-acetylphytosphingosine, tetraacetylphytosphingosine, C6-phytoceramide, sphingosine, sphingosine and the like.

Another object of the present invention is to provide an effect of suppressing the growth of skin harmful microorganisms such as Propionibacterium acnes and Staphylococcus aureus by using the above composition and an effect of suppressing inflammation caused by the action of the microorganism. It is a method to quickly recover skin damage caused by acne.

Another aspect of the present invention is a skin lotion, a cream, a soap, a body cleanser, and an ointment containing 0.01 to 30% by weight of the above composition as an active ingredient.

In the above invention, the content of the chicken egg antibody is 0.05
~ 1.0 wt%, sphingolipid content 0.05 ~ 1.5
%.

According to the present invention, there is provided a method for suppressing harmful microorganisms to the skin such as Propionibacterium acnes, eliminating skin inflammation due to acne, recovering damaged skin quickly, and treating the skin without leaving a wound. Acne treatment pharmaceutical composition, skin lotion, cream,
Can provide soap, body cleanser and ointment.

[0026]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in more detail by way of examples.

[0027]

EXAMPLES (Example 1) Separation and Purification of Chicken Egg Antibodies The method of separating and purifying hen eggs from immunized hen eggs produced by the method of the present invention is as follows. Eggs were collected from the 6th to 8th week when antibody titer increased and reached the highest level through four immunization injections, egg white and yolk were separated, and the yolk was thoroughly washed with distilled water and then frozen. . After mixing the same amount of distilled water with the frozen egg yolk, the mixture was mixed with a homogenizer at 8000 rpm for about 30 minutes to obtain a homogeneous egg yolk solution. The solution is stirred slowly while gradually adding a double volume of a 0.15% λ-carrageenan solution. After leaving the above solution for about 30 minutes 8,
After centrifugation at 000 rpm for 30 minutes, an upper layer solution was obtained. The upper layer solution was filtered using diatomaceous earth, and then filtered through a membrane filter having a molecular weight of 50,000 to obtain a water-soluble protein portion having a molecular weight of 100,000 or more. This was frozen to obtain a water-soluble protein powder having a total protein content of about 70%, in which the components of the chicken egg antibody were about 25% or more.

(Example 2) Measurement of titer of chicken egg antibody The titer of chicken egg antibody was measured by ELISA. A dry powder of Propionibacterium acnes used as an antigen was suspended at an OD of about 0.9 to 1.0 and coated for 24 hours. Use 3% bovine serum albumin at 37 ° C for about 2
The antigen-antibody reaction was carried out at 37 ° C. for 1 hour after adding the egg antibody that had been blocked and serially diluted for ３3 hours. Subsequently, a conjugate to which alkaline phosphatase was bound was added to the secondary antibody anti-chicken rabbit IgG, and reacted at 37 ° C. for 1 hour. Reaction for 2 minutes, then 2M
After stopping the reaction with a NaOH solution, the absorbance was measured.
FIG. 1 is a graph showing the measurement results using the yolk of the first week and the measurement results using the yolk of the 7th to 9th weeks for the titer of the chicken egg antibody of the present invention. As shown in FIG. 1, the specific antibody reaction titer of the egg yolk using the yolk at 7 to 9 weeks against Propionibacterium acnes was more effective than the titer of the egg yolk using the yolk at 1 week. It was about 3 to 5 times higher.

(Example 3) Antibacterial activity test of chicken egg antibody and phytosphingosine The medium used for testing the antibacterial activity of the egg antibody extracted by the above method against Propionibacterium acnes was 1 liter of distilled water and Brain Heart. Infusion 25
g, yeast extract 5g, Casitone 4g, L-cysteine hydrochloride
1g, glucose 5g, solubilized starch 1g, potassium phosphate 1
After uniformly dissolving 5 g, 1 g of ammonium sulfate, 0.2 g of magnesium sulfate, and 0.02 g of calcium chloride, the mixture was sterilized in an autoclave at 120 ° C. for 15 minutes before use. After addition antibody each to be 0,10,50,100,200ppm before inoculating bacteria was added was adjusted so that the cells to ¹⁰⁵ cells / ml. Thereafter, the cells were cultured at 37 ° C. for about 3 to 5 days under anaerobic conditions using a BBL GasPak Anaerobic System. After the culture was completed, the number of bacteria was measured to examine the antibacterial activity. Table 1 shows the number of cultured bacteria by concentration of egg antibody.

[0030]

[Table 1]

On the other hand, in order to measure the antibacterial activity of phytosphingosine against skin harmful bacteria, propionibacterium acnes and Staphylococcus a.
ureusATCC-12600) was used. Propionibacterium acnes used the above medium, and Staphylococcus medium 110 (Difco) was used to culture S. aureus.
The culture conditions were the same as those for Propionibacterium acnes, and the culture of Staphylococcus aureus was performed under aerobic conditions at 37 ° C. for about 1 to 2 days.

For the preparation of samples of phytosphingosine and its derivatives, in the case of ceramide, ceramide dissolved in isocetyl alcohol at a concentration of 1 mg / ml is used as a sample, and phytosphingosine and tetraacetylphytosphingosine are used.
hingosine), N-acetylphytosphingosine (N-ace
tylphytosphingosine) was dissolved in ethanol to a concentration of 1 mg / ml and used. After sequentially diluted by 10-fold by culturing each of the microorganisms was applied to each of the culture media the culture was determined dilution to form an approximately 10 ³ to 10 ⁴ colonies per plate medium. After culturing each microorganism, it was diluted at the dilution factor determined in the above experiment. (At this time, 0.85% NaCl was used as a diluting solution.)

The samples prepared by the above method were successively diluted with the same solvent as used for sample preparation to prepare various concentrations, and 1 ml of the diluted sample was added to 9 ml of a microorganism dilution solution and mixed well. In the case of the control group, the solvent used for dissolving the sample was used. After leaving at 37 ° C. for 30 minutes to 1 hour, 100 μL was applied to the medium (mixed occasionally), cultured under each culture condition, and after the culture was completed, the number of colonies was measured. FIG. 2 is a graph showing the antibacterial activity of ceramide, phytosphingosine and erythromycin at different concentrations against Propionibacterium acnes.

As shown in this graph, the antibacterial activity of phytosphingosine makes it impossible for Propionibacterium acnes in the culture medium to grow at a phytosphingosine concentration of 2 μg / ml or more, and about 50 μg for erythromycin. At a concentration of / ml or more, the number of Propionibacterium acnes could not grow. However, when only ceramide was added to the culture solution, no growth inhibitory effect on the number of Propionibacterium acnes was observed.

FIG. 3 is a graph showing the measured concentration and antibacterial activity of phytosphingosine, phytosphingosine lactic acid, phytosphingosine hydrochloride, C-11 ceramide and a control group against Staphylococcus aureus cells.

As shown in this graph, phytosphingosine, phytosphingosine lactic acid and phytosphingosine hydrochloride have the most remarkable inhibitory effect on the growth of the bacterial count against Staphylococcus aureus, and C-11 ceramide has a slight inhibitory effect. Appeared. In the case of the control group without any treatment, no culture inhibitory effect on Staphylococcus aureus could be observed.

Example 4 Preparation of a Cream Composition for Treating Acne The composition for protecting skin of the present invention is prepared by separately preparing a lipid layer and an aqueous layer, and then gradually adding the aqueous layer to the lipid layer.

First, the lipid layer is heated to 80 ° C. by adding 1 g of stearic acid and 2.5 g of cholesterol to 5 g of tricapride. If the mixture is completely dissolved, add 5 g of ceramide and stir until dissolved. Then, 2g of lecithin
After adding and dissolving, 1 g of oleic acid and 0.
Add 5g. In the aqueous layer, 80 g of distilled water is heated to 80 ° C.
Next, 1.5 g of phytosphingosine and its derivative were added, and about 0.45 g of lactic acid was added to completely dissolve. The mixture is stirred at 4,000 rpm for 60 minutes while gradually adding the lipid layer to the aqueous layer maintained at 80 ° C., and then cooled to 60 ° C. while cooling. To this was added a chicken egg antibody to a concentration of 0.2% to obtain a cream composition.

Example 5 Skin Microbial Inhibitory Effect of Cream Composition The skin microbial inhibiting effect of the cream composition of the present invention prepared by the above method was tested. The following method was used for the suppression test of the cream composition against microorganisms. Ten test subjects were selected, the cream composition of the present invention was applied to only one side of the face, and after 2 hours had passed, the microbial distribution on both sides of the face was examined. After wiping off both sides of the face using sterile cotton, put in sterile distilled water and stir vigorously for about 3 minutes. After stirring, 100 μl of the solution was collected and applied to a trypton agar medium, and then cultured at 37 ° C. for 24 hours.

[0040]

[Table 2]

As shown in Table 2, the chicken egg antibody of the present invention exhibited about 2 to 10 times the effect of controlling skin microbes in the case of a cream containing sphingolipid as compared with a general cream.

Example 6 Anti-inflammatory Effect of Cream Composition In order to examine the anti-inflammatory effect of the cream composition, skin kinase cells were used to examine the inhibitory effect of protein kinase C (PKC). After culturing epidermal cells to 2 × 10 ⁷ cells, the content of phytosphingosine and its derivatives was 100 u
After adding M and 400 uM, the mixture was reacted. After washing the above cells in PBS, the cells were disrupted with a homogenizer.
The cell lysate was centrifuged, and the supernatant was passed through a DE52 column to obtain a protein kinase C-containing portion. A tube to which 5 μL each of a PKC-coactivation 5X buffer, a PKC-activation 5X buffer, a PKC biotinylated peptide substrate, and a (32P) ATP mixture was added for performing an activated PKC reaction, and a PKC-coactivation 5X as a control Buffer,
Control group 5X buffer, PKC biotinylated peptide substrate, (32
P) Tubes each containing 5 μL of the ATP mixed solution were prepared, and 5 μL of the enzyme was added to each of the tubes.

After the reaction, 12.5 μL of a reaction stop solution was added to stop the reaction, and 10 μL was filtered through a SAM2TM membrane, and then 2M N
aCl once for 30 seconds, 2M NaCl three times for 2 minutes, 1% H ₃ PO ₄ and 2
The plate was washed with M NaCl solution four times for 2 minutes and twice with distilled water for 30 seconds and dried. The radioisotope was measured, and the PKC inhibitory effect was measured. As a control group, a licorice extract, which is a component often used as a stimulant for cosmetics, was used.

FIG. 4 is a graph showing the PKC inhibitory effect of the composition for treating acne containing phytosphingosine of the present invention in comparison with a licorice extract as a control group. As shown in FIG. 4, the inhibitory effect against the use of phytosphingosine (PS), tetraacetylphytosphingosine (TAPS), and N-acetylphytosphingosine (NAPS) is excellent even at a low concentration, which indicates that cosmetics, etc. It can be seen that the effect is superior to that of licorice extract, which is widely used as a stimulant.

Example 7 Clinical Experiment of Cream Composition For the clinical experiment of the above cream composition, a clinical experiment was carried out at a dermatology department of Ewha Womans University (Korea) Hospital using 30 acne patients as a control. The cream was applied twice a day after washing with soap and applied without any other topical or topical cosmetics. Clinical observations referred to the observation method of Marks and Ellis, and observed the number of closed and open comedones, papules and pustules at weekly intervals before and after treatment. In the case of the forehead, a bone sculpture section having a size of 8 × 4 cm was determined, and the center was aligned with the center line of the nose.
Also, the lower boundary of the section was matched with the point above the eyebrows.
In the case of both cheeks, a section having a size of 4 × 5 cm was determined, and the outer boundary portion was aligned with the outer eyeball protrusion, and the upper boundary portion was aligned with the lower part of the eye. The lesion observation site was always kept constant.

The results were evaluated by clinical evaluation by a patient and a physician. When the lesion was remarkably improved, it was extremely effective, when it was moderately or more improved, it was extremely effective, and when it was slightly improved, it was effective. , If there is no change at all, it is invalid and graded as 4,
A decision was made. Bacteriological observations were simultaneously made using Williamson and Kligman's Scrub method. To collect the cells, wash the affected part of the patient's face, wipe it with 75% isopropyl alcohol, dry it, attach a sterile cylindrical glassware with a diameter of 2 cm to the affected part, add 1 cc of 0.05% Triton X-100 solution, and add the tip. The solution was again collected with a sterile syringe by rubbing with a long, rounded stick for 1 minute. Brecel
The cells were inoculated into la medium, cultured at 37 ° C. for 7 days, and the cells were fixed. The clinical evaluation findings are shown in Table 3.

[0047]

[Table 3]

As shown in the above table, about 17% was very excellent, and the rate of improvement in the condition was about 80%. Comparison of the number of closed and open comedones, papules and pustules, which are clinical lesions of acne, showed that the number of lesions was distributed from 4 to 70 before treatment and the mean and standard deviation were 25 ±
There was 16.27, but after treatment it was distributed from 0 to 60, and the average and standard deviation were observed to decrease to 13 ± 10.5,
Paired T-test results were statistically significant at p <0.001. In many cases, patients themselves were evaluated as having significantly reduced inflammation. Bacterial culture was performed on 24 target patients, and only 4 of them grew Propionibacterium acnes, but the remaining 20 did not grow Propionibacterium acnes.

[0049]

Accordingly, the effect of the present invention can be attained by the combined use of a chicken egg antibody specific to acne and a sphingolipid derivative which is effective in alleviating skin inflammation and recovering the damaged skin protective film. It provides a composition that is effective for prevention and treatment, which is a non-steroidal alternative to steroid hormones and antibiotics that are currently causing many problems in acne treatment, and does not use antibiotics Providing an anti-acne agent is expected to be widely used in the pharmaceutical and functional cosmetic industries.

[Brief description of the drawings]

FIG. 1 is a graph showing the measurement results using the yolk of the first week and the measurement results using the yolk of the 7th to 9th weeks regarding the titer of the chicken egg antibody of the present invention. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-●-: Measurement result using yolk of 1st week &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-■-: Measurement result using yolk of 7th to 9th week

FIG. 2 is a graph showing the antibacterial activity of ceramide, phytosphingosine and erythromycin at different concentrations on the growth of Propionibacterium acnes.

FIG. 3 is a graph showing the antibacterial activity of phytosphingosine, phytosphingosine lactic acid, phytosphingosine hydrochloride, C11-ceramide and a control group at different concentrations on the growth of Staphylococcus aureus.

FIG. 4 is a graph showing the PKC inhibitory effect of the composition for treating acne containing phytosphingosine of the present invention in comparison with a licorice extract of a control group.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 7/00 A61K 7/00 Y 7/48 7/48 7/50 7/50 9/06 9/06 9/08 9/08 9/107 9/107 38/00 A61P 17/10 A61P 17/10 31/04 31/04 43/00 121 43/00 121 A61K 37/22 (72) Inventor Kim Jinuk Kyunggid Yoginshi Sujiup Pund Kuchulli 699 Hangku Apartment 102 Dong 306 E (72) Inventor Park Jangseong Gyeonggid Gwangchonsi Pyoland Ponland Chukon Apartment 710 Dong 401 F F Term (Reference) 4C076 AA06 AA11 AA11 AA17 BB31 CC18 CC31 DD41 DD46 DD63 DD70 4C083 AC242 AC541 AC302422 AC AC542 AC641 AC642 AD411 AD412 AD492 AD572 CC04 CC05 CC23 DD23 DD27 DD31 EE14 4C084 AA01 AA02 AA03 BA46 MA02 MA16 MA22 MA28 MA63 NA14 ZA90 ZB02 ZB35 4C085 AA13 BA10 CC04 CC07 CC26 DD41 EE03 GG10

Claims (6)

[Claims] 1. The method of claim 1, wherein the acne bacterium is Propionibacterium acnes.
A pharmaceutical composition for preventing and treating acne, comprising mixing 0.1 to 10 parts by weight of sphingolipid, which is a main component of a skin protective film, with 1 part by weight of a chicken egg antibody against es). 2. The method according to claim 1, wherein said chicken egg antibody is γ-libetin contained in a fraction having a molecular weight of 100,000 or more when a water-soluble protein portion is fractionated by a membrane filter. The pharmaceutical composition according to 1. 3. The sphingolipid according to claim 1, wherein the sphingolipid is one or more selected from ceramide 3, ceramide 4, phytosphingosine, N-acetylphytosphingosine, tetraacetylphytosphingosine, C6-phytoceramide, sphingosine, sphingosine and the like. The pharmaceutical composition according to claim 1 or 2, wherein 4. Use of the composition according to any one of claims 1 to 3 for inhibiting the growth of skin harmful microorganisms such as Propionibacterium acnes and Staphylococcus aureus and inhibiting the inflammation caused by the action of the microorganisms. How to quickly recover from skin damage caused by acne. 5. A skin lotion, cream, soap, body cleanser or ointment containing 0.01 to 30% by weight of the composition according to claim 1 as an active ingredient. 6. The method according to claim 5, wherein the content of the chicken egg antibody is 0.05.
~ 1.0 wt% and sphingolipid content is 0.05 ~ 1.5
The skin lotion, the cream, the soap, the body cleanser, and the ointment according to claim 5, which are in weight%.