JP2002121112A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin care preparation characterized by comprising an extract selected from one or more kinds of Sauropus androgynous, Andrographis paniculata and Morinda citrifolia. SOLUTION: This skin care preparation is characterized by comprising an extract selected from one or more kinds of Sauropus androgynous, Andrographis paniculata and Morinda citrifolia. The extract has excellent antioxidative action and stability. Furthermore, the skin care preparation comprising the extract has stability and excellently ameliorating actions on moisture, firmness, wrinkle and dullness of the skin.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an external preparation for skin which is excellent in improving skin moisture, firmness, wrinkles and dullness by incorporating an extract of a specific plant.

[0002]

2. Description of the Related Art In recent years, free radicals and active oxygen have been taken up as factors for oxidizing biological components, and their adverse effects have become a problem. It is said that free radicals and active oxygen are generated in the living body, decompose or crosslink biological tissues such as collagen, and oxidize fats and oils to produce lipid peroxides that damage cells. Such obstacles,
It is thought to cause aging such as reduction of skin firmness and wrinkles, and a method of adding an antioxidant for removing free radicals and active oxygen is known as one of the methods for preventing aging. Conventionally, antioxidants used for the purpose of preventing aging include ascorbic acid (vitamin C), tocopherol (vitamin E), 3,5-tert-butyl-4-hydroxytoluene (BHT), superoxide dismutase (SOD)
Etc. have been used.

[0003]

Vitamin C and superoxide dismutase used for the purpose of preventing skin aging or antioxidation are unstable, difficult to formulate, and vitamin E cannot be said to be sufficiently effective. . Also, BHT and the like, which are synthetic compounds, have a problem in safety and are limited in the blending amount. Therefore, natural materials that are stable and have few side effects are desired instead of chemically synthesized products.

[0004] In view of the above, there is a demand for an external preparation for skin that is not only safe and has excellent wrinkle and firmness-improving effects, but is also effective in improving skin moisture and dullness.

[Means for Solving the Problems]

[0005] Under such circumstances, the present inventors have conducted intensive studies, and as a result, it was found that the extract of Amamushiba, Andrographis or Yaeyamaaki was excellent in the action of preventing the oxidation of unsaturated fatty acids and the action of eliminating active oxygen, and also in terms of stability. I found it to be excellent. Furthermore, it has been found that an external preparation for skin containing an extract selected from one or more of Amamushiba, Andrographis, and Yaeyamaaki is safe and excellent in improving the moisture, firmness, wrinkles and dullness of the skin. The invention has been completed.

[0006] That is, the present invention is an external preparation for skin, characterized by containing one or more extracts selected from Amamigrass, Andrographis and Yaeyamaaki.

[0007] Amamigrass (scientific name: Saurop) used in the present invention
us androgynous) is a plant of the family Euphorbiaceae. Andrographies (scientific name: Andrographis paniculata,
Alias: Sambiloto) is a plant of the family Rhododendron.
Yaeyama Aoki (scientific name: Morinda citrifolia, aka: M
engkudu) is a plant of the family Rubiaceae. These are mainly distributed in Southeast Asia and are widely used as crude drugs.

[0008] The extract used in the present invention comprises the leaves, stems,
It is extracted from a part of a plant such as bark, flower, fruit, root or whole plant, but is not particularly limited and may be appropriately selected according to the purpose. The extraction method is not particularly limited, and may be, for example, the one extracted by heating or the one extracted at room temperature.

Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.) and liquid polyhydric alcohol (1,3-butylene glycol). ,
Propylene glycol, glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, Propyl ether). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.

The above-mentioned extract may be used as it is as an extracted solution, or may be used after being subjected to a treatment such as concentration, dilution, or filtration, if necessary. Further, the extracted solution may be subjected to a treatment such as concentration and drying, spray drying, and freeze drying to be used as a dried product.

The above-mentioned extract may be used as it is in the external preparation for skin of the present invention, and oils and fats, waxes, and the like, which are components used in general external preparations, as long as the effect of the extract is not impaired. Hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances,
Components such as humectants, powders, ultraviolet absorbers, thickeners, pigments, antioxidants, whitening agents, chelating agents and the like can also be blended.

The external preparation for skin of the present invention can be used in any of cosmetics, quasi-drugs and pharmaceuticals. Examples of the dosage form include lotions, creams, emulsions, gels,
Aerosol, ointment, poultice, essence, pack,
Those applied to the skin, such as detergents, bath agents, foundations, powders, lipsticks, etc.

The amount of the above-mentioned extract used in the present invention is 0.0001 in terms of solid based on the total amount of the external preparation for skin of the present invention.
% Or more, preferably 0.001 to 10% by weight. 0.00
If it is less than 01% by weight, a sufficient effect is hardly expected. When the content is more than 10% by weight, the effect is hardly enhanced and it is uneconomical. The method of addition may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

[0014]

EXAMPLES In order to explain the present invention in detail, examples of the production of the extract used in the present invention, prescription examples of the present invention and experimental examples will be given below, but the present invention is not limited to these examples. Absent. Parts in the amounts shown in Examples are parts by weight, and%
Indicates% by weight.

Production Example 1 Hot water extract of Amamigrass Purified water (400 mL) was added to 20 g of dried matter of Amamigrass leaves.
After extraction at 100 ° C. for 2 hours, the mixture was filtered, and the filtrate was concentrated and freeze-dried to obtain 5.0 g of a hot water extract of Amabashiba.

Preparation Example 2 50% Ethanol Extract of Amamigrass 1 L of purified water and 1 L of ethanol were added to 100 g of dried whole grass of Amamigrass, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness. As a result, 18 g of a 50% ethanol extract of Amamigrass was obtained.

Production Example 3 1,3-butylene glycol extract of Amamigrass 1,3-butyleneglycol was added to 100 g of dried amamigrass root.
After adding 1.0 kg and extracting at normal temperature for 7 days, the mixture was filtered to obtain 0.91 kg of 1,3-butylene glycol extract of Amamushiba.

Production Example 4 Hot Water Extract of Andrographis 20 g of a dried Andrographis leaf was extracted in the same manner as in Production Example 1 to obtain 3.0 g of a hot water extract of Andrography.

Preparation Example 5 A 50% ethanol extract of Andrography was extracted in the same manner as in Preparation Example 2 by extracting 100 g of a dried plant of Andrography's whole plant to obtain 12 g of a 50% ethanol extract of Andrography.

Production Example 6 1,3-butylene glycol extract of Andrographis 100 g of dried dried Amamigrass root was extracted in the same manner as in Production Example 3 to obtain 0.93 kg of a 1,3-butylene glycol extract of Andrographis. .

Production Example 7 Hot Water Extract of Yaeyama Aoki The dried product of leaves of Yaeyama Aoki was extracted in the same manner as in Production Example 1 to obtain 3.2 g of a hot water extract of Yaeyama Aoki.

Preparation Example 8 50% Ethanol Extract of Yaeyama Aoki 100 g of dried whole plant of Yaeyama Aoki was extracted in the same manner as in Production Example 2 to obtain 23 g of a 50% ethanol extract of Yaeyama Aoki.
Obtained.

Production Example 9 1,3-butylene glycol extract of Ayamayama oki 100 g of dried yaeyamaaki root was extracted in the same manner as in Production Example 3 to obtain 0.93 kg of a 1,3-butyleneglycol extract of Ayamayamaoki.

Example 1 Cream 1 Prescription Formulation Amount 1. Hot water extract of Amamushiba (Preparation Example 1) 1.0 part 2. Squalane 5.5 3. Olive oil 3.0 4. Stearic acid 2.0 5. Beeswax 2.0 6. Octyldodecyl myristate 3.5 7. Polyoxyethylene cetyl ether (20E.O.) 3.0 8. Behenyl alcohol 1.5 9. Glycerin monostearate 2.5 10. Fragrance 0.1 11. 1,3-butylene glycol 8.5 12. Ethyl parahydroxybenzoate 0.05 13. Paraoxybenzoic acid Methyl 0.2 14. Make the total amount 100 with purified water. [Production method] Heat and dissolve components 2 to 9 and mix. Maintain at 70 ° C to obtain an oil phase. Components 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. Add the water phase to the oil phase, emulsify, cool with stirring, add component 10 at 45 ° C,
Cool to 30 ° C to obtain a product.

Example 2 Cream 2 Cream 2 was prepared in the same manner as in Example 1 except that the hot water extract of Amamigrass was replaced by a hot water extract of Andrographis (Production Example 4).

Example 3 Cream 3 Cream 3 was prepared in the same manner as in Example 1 except that the hot water extract of Amamushiba was replaced with the hot water extract of Yaeyama Aoki (Production Example 7).

COMPARATIVE EXAMPLE 1 Conventional Cream A conventional cream was prepared by replacing the hot water extract of Amamushiba with purified water in Example 1.

Example 4 Lotion Formulation Formulation amount 1. 0.1% of 50% ethanol extract of Amamushiba (Production Example 2) 2. 50% ethanol extract of Andrographis (Production Example 5) 0.1 3. 50% ethanol of Yaeyama Aoki Extract (Production Example 8) 0.1 4. 1,3-butylene glycol 8.0 5. Glycerin 2.0 6. Xanthan gum 0.02 7. Citric acid 0.01 8. Sodium citrate 0.1 9. Ethanol 5.0 10. Methyl parahydroxybenzoate 0.1 11. Poly Oxyethylene hydrogenated castor oil (40E.O.) 0.1 12. Appropriate amount of fragrance 13. Make the total amount 100 with purified water [Production method] Components 1 to 8 and 13 and components 9 to 12 are each dissolved uniformly. Both are mixed and filtered to obtain a product.

Example 5 Emulsion Prescription Formulation Amount 1. 0.5 part of 1,3-butylene glycol extract of Amamushiba (Production Example 3) 2. Olive oil 5.0 3. Jojoba oil 5.0 4. Cetanol 1.5 5. Glycerin monostearate 2.0 6 Polyoxyethylene cetyl ether (20E.O.) 3.0 7. Polyoxyethylene sorbitan monooleate (20E.O.) 2.0 8. Fragrance 0.1 9. Propylene glycol 1.0 10. Glycerin 2.0 11. Methyl paraoxybenzoate 0.2 12 Adjust the total amount to 100 with purified water. [Production method] Components 2 to 7 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Components 1 and 9 to 12 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. Add the water phase to the oil phase, emulsify, cool with stirring, add component 8 at 45 ° C, and add
Cool to 0 ° C to obtain a product.

Example 6 Gel Formulation Formulation Amount 1. Andrographics 1,3-butylene glycol (Production Example 6) 0.01 part 2. Ethanol 5.0 3. Methyl parahydroxybenzoate 0.1 4. Polyoxyethylene hydrogenated castor oil (60E) .O.) 0.1 5. Appropriate amount of perfume 6. 1,3-butylene glycol 5.0 7. Glycerin 5.0 8. Xanthan gum 0.1 9. Carboxyvinyl polymer 0.2 10. Potassium hydroxide 0.2 11. Make the total amount 100 with purified water [ Production method] Components 2 to 5, and components 1 and 6 to 11 are each dissolved uniformly, and both are mixed to form a product.

Example 7 Ointment Prescription Formulation amount 1. Hot water extract of Amamushiba (Production Example 1) 1.0 part 2. Hot water extract of Andrographis (Production Example 4) 1.0 3. Polyoxyethylene cetyl ether (30E. O.) 2.0 4. Glycerin monostearate 10.0 5. Liquid paraffin 5.0 6. Cetanol 6.0 7. Methyl parahydroxybenzoate 0.1 8. Propylene glycol 10.0 9. Make the total amount to 100 with purified water. 6 is dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Components 1, 2 and 7 to 9 are dissolved by heating and mixed, and the mixture is kept at 75 ° C. to form an aqueous phase. Add the water phase to the oil phase, emulsify and cool to 30 ° C with stirring to obtain the product.

Example 8 Pack Formulation Compounding amount 1. 0.1 part of 50% ethanol extract of Andrographis (Production Example 5) 2. Hot water extract of Yaeyama Aoki (Production Example 7) 0.1 3. Polyvinyl alcohol 12.0 4. Ethanol 5.0 5. 1,3-butylene glycol 8.0 6. Methyl paraoxybenzoate 0.2 7. Polyoxyethylene hydrogenated castor oil (20E.O.) 0.5 8. Citric acid 0.1 9. Sodium citrate 0.3 10. Perfume qs 11. Purified water [Production method] Components 1 to 11 are uniformly dissolved to give a product.

Example 9 Foundation Formulation Compounding amount 1. 1.0 part of 1,3-butylene glycol extract of Aeyama aki (Production Example 9) 2. Stearic acid 2.4 3. Polyoxyethylene sorbitan monostearate (20E.O.) 1.0 4. Polyoxyethylene cetyl ether (20E.O.) 2.0 5. Cetanol 1.0 6. Liquid lanolin 2.0 7. Liquid paraffin 3.0 8. Isopropyl myristate 6.5 9. Butyl paraoxybenzoate 0.1 10. Sodium carboxymethyl cellulose 0.1 11. Bentonite 0.5 12. Propylene glycol 4.0 13. Triethanolamine 1.1 14. Methyl parahydroxybenzoate 0.2 15. Titanium dioxide 8.0 16. Talc 4.0 17. Bengala 1.0 18. Yellow iron oxide 2.0 19. Fragrance qs. 20. Purified water [Production method] Components 2 to 9 are heated and dissolved, and kept at 80 ° C to obtain an oil phase. Swell the ingredient 10 well to the ingredient 20, then add the ingredient 1
And 11 to 14 are added and mixed uniformly. Add the ingredients 15 to 18 crushed and mixed with a crusher to this, and stir with a homomixer for 75
Keep at ℃ and make water phase. Add the oil phase to this aqueous phase with stirring, cool, add component 19 at 45 ° C, and stir with 30
Cool to ℃ to make the product.

Example 10 Bath agent Prescription Formulation amount 1. 50% ethanol extract of Amamushiba (Production Example 2) 5.0 parts 2. Sodium bicarbonate 50.0 3. Yellow 202 (1) qs 4. Perfume qs 5. Sulfuric anhydride The total amount is adjusted to 100 with sodium. [Production method] Components 1 to 5 are uniformly mixed to obtain a product.

Next, experimental examples will be described in order to explain the effects of the present invention in detail.

EXPERIMENTAL EXAMPLE 1 Method for Measuring Unsaturated Fatty Acid Antioxidant Activity (Antioxidant Activity) A sample 0.5 mg (per solid), 0.02 mg / mL linoleic acid / ethanol solution 2 mL, ethanol 1 mL and 0.2 M phosphate buffer ( Adjust the volume to 1 mL with purified water (pH 7.0). The reaction was carried out at 50 ° C. for 8 days to prepare a test solution, and the absorbance was measured by the rodin iron method. That is, 0.1mL of each test solution is 4.7mL of 75% (v / v) ethanol, 0.1mL of 30% ammonium thiocyanate aqueous solution
And 0.1 mL of 0.02 M ferrous chloride / hydrochloric acid solution was added, and the mixture was allowed to stand for 3 minutes, and then the absorbance (A) at a wavelength of 500 nm was measured. On the other hand, the same operation was performed using a test solution to which no sample was added as a blank, and the absorbance (B) was measured. In addition, vitamin E was used as a comparative product. The unsaturated fatty acid oxidation preventing action of each sample was calculated by the following equation. Unsaturated fatty acid oxidation prevention rate (%) = (1-A ÷ B) × 10
0

Table 1 shows the results of these tests. As a result, the extracts of Amamushiba, Andrographis and Yaeyamaaki showed excellent antioxidant action of unsaturated fatty acids.

[0038]

[Table 1]

Experimental Example 2 Active oxygen scavenging action (antioxidant action) Using Production Examples 1 to 9 as samples, the scavenging action of superoxide, a kind of active oxygen, was measured. Superoxide dismutase (SOD) was used as a positive control. In addition, in order to confirm the stability of the sample, the same measurement was performed using a solution obtained by heating an aqueous solution of the sample at 95 ° C. for 1 hour.

Method of measuring active oxygen scavenging action 0.45 mL of a coloring reagent (0.24 mM
Nitro blue tetrazolium, containing 0.4 mM xanthine
0.1 M phosphate buffer; pH 8.0 and 0.45 mL enzyme solution (0.1 U / mL)
Xanthine oxidase) was added and reacted at 37 ° C. for 20 minutes to produce diformazan. After adding 1.0 mL of a reaction stop solution (69 mM sodium dodecyl sulfate aqueous solution) to this solution, the absorbance (At) at a wavelength of 560 nm was measured. Similarly, a blank solution (0.1 M phosphate buffer; pH
8.0) was added thereto for reaction, and the absorbance (As) was measured. On the other hand, the same operation was performed using purified water instead of the sample solution as a control, and the absorbances (Bt) and (Bs) were measured. The active oxygen scavenging rate of each sample was calculated by the following equation. Active oxygen scavenging rate (%) = {1- (As-At)} (Bs
−Bt)｝ × 100

Table 2 shows the results of these tests. Also,
For SOD, the active oxygen scavenging effect was greatly reduced by heating at 95 ° C. for 1 hour, whereas the extract of Amamushiba, Andrographis and Yaeyamaaki was not changed. From the above, the extracts of Amamushiba, Andrographis and Yaeyamaaki showed stable and excellent active oxygen scavenging action even when heated at 95 ° C.

[0042]

[Table 2]

EXPERIMENTAL EXAMPLE 3 Use Test Using the creams of Examples 1 to 3 and the conventional cream of Comparative Example 1, 30 women (30 to 45 years old) were used as subjects.
A month-long use test was performed. After use, a questionnaire survey was conducted to comprehensively judge the improvement of skin moisture, firmness, wrinkles and dullness.

As a result, the external preparation for skin showed an excellent effect on improvement of skin moisture, firmness, wrinkles and dullness (Table 3). During the test period, there was no skin trouble and there was no problem in safety. Also, there was no problem with the deterioration of the prescription components.

[0045]

[Table 3]

Also used for the lotion of Example 4, the emulsion of Example 5, the gel of Example 6, the ointment of Example 7, the pack of Example 8, the foundation of Example 9, and the bath agent of Example 10. The test showed that it was safe and had an excellent effect of improving skin moisture, firmness, wrinkles and dullness.

[0047]

From the above, it can be seen from the above that the amamigrass of the present invention,
One or more extracts selected from Andrographis and Yaeyamaaki were excellent in antioxidant action and stability. In addition, skin external preparations containing these extracts,
It was safe and excellent in improving skin moisture, firmness, wrinkles and dullness.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI theme coat ゛ (reference) A61P 43/00 107 A61P 43/00 107 F term (reference) 4C083 AA082 AA111 AA112 AA122 AB232 AB242 AB312 AB352 AB432 AB442 AC022 AC072 AC102 AC122 AC182 AC242 AC302 AC342 AC422 AC432 AC442 AC482 AC542 AC842 AD112 AD272 AD352 CC04 CC05 CC07 CC12 CC25 DD21 DD32 DD41 EE12 FF01 4C088 AB14 AB46 AC01 AC03 AC04 AC05 AC11 CA05 CA06 CA09 MA63 ZA89 ZB22

Claims (3)

[Claims] 1. An external preparation for skin, characterized by containing an extract selected from one or more of amamishiba, andrography, and yaeyamaaki. 2. The external preparation for skin according to claim 1, wherein the extract is an extract using one or more solvents selected from water, lower alcohols and liquid polyhydric alcohols. 3. An anti-aging agent comprising an extract selected from one or more selected from the group consisting of Amamushiba, Andrographis, and Yaeyamaaki.