JP2002121110A - Cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a cosmetic comprising a new bleaching component exhibiting excellently preventing and symptom ameliorating effects on skin pigmentation such as spot, freckle, etc., and high skin stability. SOLUTION: This cosmetic is obtained by formulating a cosmetic with an extract of a plant of the genus Pandanus of the family Pandanaceae.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to cosmetics which exhibit excellent skin whitening and skin whitening effects on the skin and are useful as basic cosmetics, makeup cosmetics, bath agents and the like.

[0002]

2. Description of the Related Art Various whitening agents have been proposed for the purpose of preventing or improving pigmentation, particularly spots and freckles caused by aging or aging, and keeping the skin beautiful and healthy. And cosmetics containing them are on the market. However, with these conventional whitening agents, it is difficult to sufficiently satisfy both of the whitening / beautifying effect and the skin safety, and a new cosmetic containing such an improved whitening agent as an active ingredient is required. Have been.

[0003]

In view of the above problems of the prior art, the present inventors have conducted intensive studies to find new whitening active ingredients derived from natural products from the viewpoint of skin safety. The present inventors have found that an extract of a specific plant exhibits a remarkable tyrosinase activity-inhibiting action, thereby making it possible to provide a cosmetic having an excellent whitening / beautifying effect and excellent skin safety, and completed the present invention.

[0004]

That is, the present invention relates to a cosmetic comprising an extract of a plant belonging to the genus Taconium, which belongs to the genus Taconidae.

Hereinafter, the present invention will be described in detail. Examples of the plants belonging to the genus Taconidaceae used in the present invention include, for example, Pandanus amariforius (Pandanus amary)
llifolius Roxb.), P. andamanensium Kurz., P. boninensis Weber., Pandanus conoidea.
(P. conoidea Lamck.), Pandanus Edulis (P. e
dulis Thouars.), P. kafu Mar
t.), P. odorus Ridl., P. tectorius Soland., P. utilis Bory., and the like. Among them, from the viewpoint of effectiveness, Pandanus
The use of Amariliforius is particularly preferred.

[0006] The preparation of the extracts of the plants of the genus Octopus can be performed by preparing appropriate parts of the plant such as whole plants, leaves, stems, roots and seeds;
Preferably, leaves are used, and this is generally washed with water, dried,
It is carried out by slicing and immersing in an extraction solvent. Examples of the extraction solvent include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; ethyl acetate, butyl acetate, and methyl propionate. Esters such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene and chloroform, among which water or water and lower grades are particularly preferred. It is preferable to use a mixture of alcohols or a mixture of water and polyhydric alcohols.

The extraction temperature and time vary depending on the type of plant used, the degree of shredding, the type of extraction solvent, etc., but are generally from 5 to 80 ° C. for 2 hours to 7 days, especially from 20 to 40 ° C. for 6 to 6 days.
It is preferable to perform the extraction for 48 hours. The extract solution obtained here is usually adjusted to a pH of 4 to 8, and then used as it is or at an appropriate concentration by vacuum concentration or the like. In some cases, the powder may be used in the form of powder according to a conventional method such as a spray drying method or a freeze drying method.

[0008] The amount of the above-mentioned extract in the cosmetic of the present invention is generally 0.001 to 0.003 as solid content in the case of basic cosmetics such as creams, emulsions and lotions.
0.5% by weight, preferably in the range of 0.01 to 0.1% by weight, and in the case of makeup cosmetics such as blush, white powder,
Generally 0.001 to 0.5% by weight, preferably 0.01 to 0.1% by weight
In the case of a bath agent, generally 0.01 to 5
%, Preferably in the range of 0.1 to 1% by weight.

The cosmetic of the present invention contains, in addition to the extract of the plant belonging to the genus Taconium, an ingredient commonly used in cosmetics,
For example, oily components, surfactants, humectants, thickeners, preservatives
A bactericide, a powder component, an ultraviolet absorber, an antioxidant, a dye, a fragrance and the like are appropriately compounded as required.

Here, the oily component includes, for example, plant-derived oils and fats such as olive oil, jojoba oil, castor oil, soybean oil, coconut oil, palm oil, cacao oil, and meadow home oil; mink oil, turtle oil and the like. Animal-derived fats and oils; waxes such as beeswax, carnauba wax and lanolin; hydrocarbons such as liquid paraffin, vaseline, paraffin wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid; Higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol; synthetic esters such as isopropyl myristate, butyl oleate, and 2-ethylhexyl glyceride; and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, and polyoxyethylene cured castor. Non-ionic surfactants such as oils and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkyl benzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ethers Sulfate, polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, polyoxyethylene alkyl phenyl ether phosphate Quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts , N, N-dialkylmorphonium salts, cationic surfactants such as polyethylenepolyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammonio betaine;
N, N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N ',
And amphoteric surfactants such as N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-
Examples include butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and further include saccharides, hyaluronic acid, lactic acid, urea, higher fatty acid octyldodecyl, various amino acids, and derivatives thereof.

Examples of the thickener include polysaccharides such as carrageenan, alginic acid, pectin, and locust bean gum; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; polyvinyl alcohol, polyvinyl pyrrolidone; Synthetic polymers such as carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives;

Examples of preservative disinfectants include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate and butyl paraoxybenzoate; phenoxyethanol, dichlorophen, hexachlorophen, chlorhexidine hydrochloride, Examples include benzalkonium chloride, salicylic acid, ethanol, undecylenic acid, phenols, and jamal (imidazodeinyl urea).

Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder and the like.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, ethylhexyl paramethoxycinnamate, octyl cinnamate, 2,4-dihydroxybenzophenone, 2-hydroxy-4- Methoxybenzophenone-5-sulfonate, 2- (2-hydroxy-5-methylphenyl) benzotriazole,
There are urocanic acid, ethyl urocanate and the like.

Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and derivatives thereof. Further, as other components, lecithins, silk-related substances and the like can be blended.

The cosmetic of the present invention may further contain other physiologically active ingredients as long as the effectiveness of the combination of the present invention is not impaired. Such compounds include, for example, kojic acid and its derivatives (eg, kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, etc.) and arbutin, ascorbic acid Acids and derivatives thereof [for example, L-ascorbic acid-2
-Ascorbic acid ester salts such as phosphoric acid ester salt and L-ascorbic acid-2-sulfate ester salt (the salt is sodium salt, magnesium salt and the like); L-ascorbic acid-2-glucoside (2-O-α-D- Ascorbic acid glycosides such as glucopyranosyl-L-ascorbic acid) and L-ascorbic acid-5-glucoside (5-O-α-D-glucopyranosyl-L-ascorbic acid), ellagic acid, resorcinol derivatives (for example, , 4-n-butyl resorcinol, 4-isoamyl resorcinol, etc.), somaria extract, saxifrage extract, rice bran extract, 2,5-dihydroxybenzoic acid derivative (e.g., 2,
Whitening ingredients such as 5-diacetoxybenzoic acid); placental extract, collagen, nicotinic acid and its derivatives (eg, nicotinamide, benzyl nicotinate, etc.), vitamin E
And derivatives thereof (eg, vitamin E nicotinate, vitamin E linoleate, etc.), α-hydroxy acids (eg, lactic acid, citric acid, α-hydroxyoctanoic acid, etc.), diisopropylamine dichloroacetate, γ-amino-β-
Hydroxy lactic acid and other ingredients for preventing skin aging and improving skin roughness;
There are crude drug extracts such as gentian extract.

Next, the present invention will be described more specifically with reference to Production Examples, Examples (Formulation Examples) and Test Examples, but the invention is not limited thereto. In the following, all parts are parts by weight, and all parts are by weight.

Production Example 1 Preparation of an extract solution of a plant belonging to the genus Octopus (1) Dried leaves of Pandanus amariliforius were about 5 m
m, and immersed in 500 g of purified water,
After sufficient stirring, the mixture was allowed to stand at room temperature for 18 hours. Next, this was filtered to obtain 350 ml of a brown transparent extract solution (solid content concentration: 1.02%).

Preparation Example 2 Preparation of an extract of a plant of the genus Octopus (2) In Preparation Example 1, a mixed solution of purified water / ethanol = 80/20 (V / V) was used instead of purified water as the extraction solvent. In the same manner as in Production Example 1, 380 ml of a brown transparent extract solution was obtained (solid content: 0.65%).

Preparation Example 3 Preparation of an extract of a plant of the genus Taconium (3) In Preparation Example 1, Pandanus sp.
350 ml of a brown and transparent extract solution was obtained in the same manner as in Production Example 1 except that Pandanus techtolius was used instead of Amariliforius (solid content: 1.00%).

Preparation Example 4 Preparation of an extract of a plant of the genus Taconium (4) In Preparation Example 1, Pandanus sp.
360 ml of a brown transparent extract solution was obtained in the same manner as in Production Example 1 except that Pandanus andamanendium was used instead of Amariliforius (solid content: 0.95).
%).

Preparation Example 5 Preparation of powder extract of Aspergillus plant extract The extract solution obtained in the same manner as in Preparation Example 1 was concentrated to 35 ml and freeze-dried to obtain 3.5 g of extract powder.

Example 1 Cream [A component] part Liquid paraffin 5.0 hexalane [glyceryl trioctanoate manufactured by Technoble Co., Ltd.] 4.0 paraffin 5.0 glyceryl monostearate 2.0 polyoxyethylene (20) sorbitan Monostearate 6.0 Butylparaben 0.1 [Component B] Extract solution of Production Example 1 5.0 Glycerin 5.0 Carboxymethylmonostearate 0.1 Methylparaben 0.1 Moiston C [Technol Co., Ltd.] NMF Component] 1.0 Amount of purified water to make the total amount 100 parts [Component C] Perfume Appropriate Amount The above components A and B were each heated to 80 ° C. or higher, and then stirred and mixed. After cooling to 50 ° C., the C component was added and further stirred and mixed to prepare a cream.

Example 2 Emulsion [Component A] Part Liquid paraffin 6.0 Hexaran 4.0 Jojoba oil 1.0 Polyoxyethylene (20) sorbitan monostearate 2.0 Soybean lecithin 1.5 Methylparaben 0.15 Ethylparaben 0.03 [Component B] Extract solution of Production Example 2 2.5 Glycerin 3.0 1,3-butylene glycol 2.0 Carboxymethylcellulose 0.3 Sodium hyaluronate 0.01 Purified water Amount that makes the total amount 100 parts [Component C] Perfume Appropriate amount After heating the above components A and B to 80 ° C. or higher, they were mixed by stirring. After cooling to 50 ° C., the C component was added and further stirred and mixed to prepare an emulsion.

Example 3 Lotion [Component] Part Extract solution of Production Example 3 5.0 Ethanol 10.0 Glycerin 3.0 1,3-butylene glycol 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Carboxyvinyl polymer 0.1 Appropriate amount of perfume Purified water An amount that gives a total amount of 100 parts The above components were mixed to prepare a lotion.

Example 4 Pack [Ingredients] Part Polyvinyl alcohol 15.0 Hydroxymethylcellulose 5.0 Propylene glycol 5.0 Ethanol 10.0 Methylparaben 0.2 Extract solution of Production Example 4 5.0 Perfume Appropriate amount Purified water A pack was prepared by mixing the above components with 100 parts.

Example 5 Press Powder [Component A] Part Bengala 0.5 Yellow Iron Oxide 1.5 Black Iron Oxide 0.1 Titanium Oxide 10.0 Nylon Powder 4.0 Sericite Amount of 100 parts of Mica 23 0.0 Talc 25.0 Extract powder of Production Example 5 0.2 [Component B] Squalane 1.0 Methylpolysiloxane 4.0 Propylparaben 0.1 Dehydroacetic acid 0.1 Liquid paraffin 2.0 Perfume Appropriate A The component and the component B were mixed and stirred, respectively, and then sieved through a 200 mesh Tyler mesh sieve, and the obtained mixed powder was pressed into a mold to prepare a uniform press powder.

Example 6 Bath Agent [Components] Part Extract powder of Production Example 5 0.5 Sodium sulfate 47.0 Sodium hydrogen carbonate Amount that total amount becomes 100 parts Perfume Appropriate amount The above components are mixed and stirred to obtain a uniform bath. An agent was prepared.

Comparative Example Cream A cream was prepared in the same manner as in Example 1 except that 1.0 part of kojic acid was used in place of 5.0 parts of the extract of the plant belonging to the genus Octopus in Production Example 1. Prepared.

Comparative Example Cream A cream was prepared in the same manner as in Example 1 except that the extract of the plant of the genus Octopus was not used.

Test Example 1 Inhibitory Effect of Intracellular Tyrosinase Activity [Test Method] Cultured B16 mouse melanoma cells were
1 × 104 / well seeded in a well microplate, 10%
Eagle minimum essential medium containing calf serum (FBS) (ME
After pre-culturing for 1 day in M) at 37 ° C. and 5% CO 2, the culture solution was added so that the extract solution of Production Example 1 had a concentration of 2.5 or 5.0%. The cells were replaced with Eagle MEM containing% FBS and cultured under the same conditions for 2 days. Next, the culture solution is removed, and a surfactant (Triton X-10) is removed.
0) was added to the cell treatment solution, 5 mM L-dopa or 0.2% MTT was added, and a tyrosinase reaction was performed at 37 ° C., followed by a microplate reader (Model 1).
450, manufactured by Bio-Rad Inc.), and the dopa value was measured at a wavelength of 490 nm, and the MTT value was measured at 570-630 nm. For comparison, the same test was performed for the case where 2 mM kojic acid was added instead of the extract solution of Production Example 1 and the case where no sample was added (blank).

[Results] The dopa value obtained in the above test is shown in FIG. 1, and the MTT value is shown in FIG. From the results of FIGS. 1 and 2, it can be seen that the extract of the plant of the genus Octopus according to the present invention significantly suppresses intracellular tyrosinase activity without a decrease in cell activity.

Test Example 2 Pigmentation Inhibition Test The in vivo whitening effect of an extract of the plant of the genus Octopus according to the present invention was evaluated by a pigmentation inhibition test using guinea pigs. [Test Method] The hair of a colored guinea pig (male, 8 weeks of age) at the center of the back at 60 mm in the vertical direction and 30 mm in the horizontal direction was shaved, and this part was divided into two right and left parts. A liquid (sample solution) obtained by diluting the extract solution of Preparation Example 1 with purified water to a concentration of 5.0% in one of the compartments, and purified water as a control in the other, 5 ml each in the morning and evening, respectively. Apply for 6 days, and apply 500 mJ / cm 2 of U to the application site once daily immediately before application in the morning.
The sample was irradiated with VB, and the state of pigmentation at the irradiated site was visually observed on the evening of the sixth day, and evaluated according to the following criteria.

[Evaluation Criteria for Pigmentation]-: No pigmentation observed ±: Slight pigmentation observed +: Mild pigmentation observed 2+: Moderate pigmentation observed 3+: Severe pigmentation observed

[Results] The results are shown in Table 1.

[Table 1]

As is clear from the results shown in Table 1, the extract of the genus Octopus according to the present invention has an effect of remarkably inhibiting pigmentation on the skin due to exposure to ultraviolet rays. Test Example 3 Skin irritation evaluation test The skin irritation of various whitening ingredients was evaluated by a neutral red uptake test using cultured rabbit corneal cells (SIRC), which is an alternative method to the ocular mucosal irritation test.

[Samples] (1) Extract powder of Production Example 5 (2) Kojic acid (3) Arbutin

[Test Method] SIRC was seeded at 5,000 cells / well in a 96-well microplate, and cultured in Eagle's minimum essential medium (MEM) containing 5% calf serum for 3 days. And then add 2
Cultured for days. Next, SIRC was cultured in an Eagle MEM medium containing 0.05% neutral red (NR) for 3 hours, the NR uptake rate was determined for the obtained SRIC, and the skin irritation of each sample was evaluated from the value. .

[Results] The results are shown in Table 2.

[Table 2]

As shown in Table 2, the extract of the plant of the genus Octopus according to the present invention is less irritating to the skin than existing whitening agents, and can provide cosmetics excellent in safety.

Test Example 4 Monitor Test Each of the creams of Example 1, Comparative Example and Control Example was subjected to a monitor test to examine its inhibitory effect on skin pigmentation and skin irritation. [Test method] Twenty women aged 18 to 50 years randomly selected as subjects were given each cream twice a day (morning,
Night), and applied to the left upper arm for one month, the state of pigmentation of the applied part and the erythema of the skin were visually observed, and evaluated based on the following evaluation criteria.

[Evaluation Criteria] (Pigmentation) A: disappeared B: apparently decreased C: somewhat decreased D: hardly changed E: increased rather (erythema) A: difference from control No: B: almost no difference from control example C: erythema is slightly more noticeable than control example D: considerable erythema is more noticeable than control example E: erythema is clearly noticeable compared to control example

[Results] The results are shown in Table 3.

[0046]

[Table 3]

As shown in Table 3, the cream containing the plant extract of the genus Octopus according to the present invention has an excellent effect of preventing pigmentation, and is highly safe with little irritation to the skin.

[0048]

Industrial Applicability The cosmetic of the present invention comprising an extract of a plant belonging to the genus Taconium of the octopus family has a strong tyrosinase activity inhibitory action of the extract of the active ingredient.
Not only does it significantly suppress or reduce pigmentation of the skin such as spots and freckles, and since the extract is derived from natural products, it is less irritating to the skin and is excellent in safety.

[Brief description of the drawings]

FIG. 1 is a graph showing the dopa value of each sample of Test Example 1 (vertical axis: dopa value).

FIG. 2 is a graph showing the MTT value of each sample of Test Example 1 (vertical axis: MTT value).

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Yosuke Haneda 1-6-8 Kitahorie, Nishi-ku, Osaka Kyoei Chemical Industry Co., Ltd. F-term (reference) 4C083 AA111 AA112 AA122 AB232 AB242 AB312 AB352 AB432 AB442 AC012 AC022 AC102 AC122 AC302 AC392 AC422 AC442 AC482 AC842 AD072 AD112 AD152 AD272 AD332 AD572 CC04 CC05 CC07 CC25 DD17 DD31 EE16

Claims (2)

[Claims] 1. A cosmetic comprising an extract of a plant belonging to the genus Taconium of the family Taconidae. 2. The plant belonging to the genus Taconidaceae belonging to the octopus family Pandanus amaryllifol (Pandanus amaryllifol).
ius Roxb.).