JP2002003390A - Fibroblast growth agent, food and drink for beauty culture and skin cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To find out a substance effective in promoting the growth of fibroblasts and a substance having skin beautifying actions or aging preventing actions on the skin and to obtain a fibroblast growth agent, a food and drink for beauty culture and a skin cosmetic comprising either one of the above substances as an active ingredient. SOLUTION: The fibroblast growth agent, skin cosmetic or food and drink for beauty culture is obtained by including an extract from a leaf and/or a stem of Alpinia nutans or Alpinia speciosa.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a fibroblast-proliferating agent containing an extract from a plant as an active ingredient, and a cosmetic food or drink and a skin cosmetic containing the extract from a plant.

[0002]

2. Description of the Related Art The epidermis and dermis of skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as collagen outside these cells and supporting the skin structure.

[0003] In young skin, fibroblasts are actively proliferating, and the interaction of skin tissues such as fibroblasts and collagen secures water retention, flexibility, elasticity, and the like. It is maintained in a glossy and fresh state.

However, there are certain external factors such as ultraviolet rays, remarkable drying of air, and excessive skin washing,
As aging progresses, the proliferation of fibroblasts slows down and the moisturizing function and elasticity of the skin and the like decrease. Then, the skin loses its tone and luster, and exhibits aging symptoms such as roughness and wrinkles.

[0005] The most common practice for preventing and improving skin aging by the above mechanism is protection of the skin surface. For example, by applying an external preparation having a moisturizing effect such as a lotion, a cream, and a milky lotion to the skin,
Evaporation of water from the skin is prevented, the water content of the skin is maintained, and the skin is moisturized.

However, such an external preparation only improves the keratin condition of the epidermis, and cannot be expected to improve the tension holding mechanism in the dermis. Also, since moisturizers slow the evaporation of water from the skin,
In general, the feeling of use is poor, and even long-term use may even cause skin damage.

[0007]

SUMMARY OF THE INVENTION Therefore, the present invention firstly finds a substance having an action of promoting the proliferation of fibroblasts that synthesize collagen, and uses the substance as an active ingredient to act as a fibroblast proliferation agent. The purpose is to provide. Another object of the present invention is to provide a cosmetic food / beverage product and a skin cosmetic capable of exhibiting a beautifying effect or preventing skin aging by promoting the proliferation of fibroblasts that synthesize collagen. I do.

[0008]

Means for Solving the Problems To achieve the above object, the present invention firstly provides a fibroblast characterized by containing an extract from the leaves and / or stems of moon peach as an active ingredient. Secondly, the present invention provides a cell proliferation-promoting agent, and secondly, a cosmetic food / beverage product and a skin cosmetic comprising an extract from moon peach leaves and / or stems. In a preferred embodiment of the agent for acting on fibroblast proliferation of the present invention, the extract is an extract obtained using a polar solvent as an extraction solvent. In a preferred embodiment of the fibroblast proliferation-promoting agent of the present invention, the polar solvent is water, methanol, ethanol, propanol, 1,3-butylene glycol, glycerin, propylene glycol or a mixture of two or more thereof. is there.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.

[Fibroblast Proliferating Agent] The fibroblast growing agent of the present invention is characterized by containing an extract from leaves and / or stems of moon peach as an active ingredient.

[0011] In the agent for promoting fibroblast proliferation of the present invention,
The “extract” includes an extract obtained from an extraction raw material by an extraction treatment, a diluent or a concentrate of the extract, a dried product obtained by drying the extract, or a crude product or a purified product thereof. Both are included.

[0012] In the agent for promoting fibroblast proliferation of the present invention,
What is used as the extraction raw material during the extraction process is the above-ground portion of the moon peach, especially the leaves and / or stems of the moon peach.

Moon peach belongs to the genus Alpinia of the ginger family,
It is a perennial evergreen plant distributed from southern Kyushu to India. Accordingly, moon peach is readily available from these areas. Moon peaches generally have a height of about 3 m, oval lanceolate leaves, a length of 40 to 70 cm, a width of about 15 cm, and a dark green color. Moon peach leaves have a unique aroma, and essential oils are extracted from the leaves and used as insect repellents and fungicides for mites and molds, or stems are used as fibers. Moon peaches include moon peaches (Alpinia nutans or Alpinia
speciosa), Tyrin moon peach (Alpinia urarensis Hay.),
Filey moon peach (Alpinia sanderae Sand.), Yakuchi (Alpinia
oxyphylla L.) and Taiwan moon peach (Alpinia sp.). In the agent for promoting fibroblast proliferation of the present invention,
The type and place of production of the moon peach used as the extraction raw material are not particularly limited, but the moon peach (Alpinia nutans or Alpinia spe
ciosa) is preferred.

In the agent for promoting fibroblast proliferation of the present invention,
The leaves and / or stems of the moon peach used as the raw material for extraction are
It is preferable to dry-grind or coarsely crush in advance. The leaves and / or stems of the moon peach are suitably dried and pulverized immediately after collection. Drying may be performed in the sun or using a commonly used dryer.

The leaves and / or stems of the moon peach are made of hexane,
A material subjected to a pretreatment such as degreasing with a nonpolar solvent such as benzene may be used as an extraction raw material. By performing a pretreatment such as defatting, the leaves and / or stems of the moon peach can be efficiently extracted with a polar solvent.

[0016] In the agent for promoting fibroblast proliferation of the present invention,
In the case of the extraction treatment, it is preferable to use a polar solvent as the extraction solvent. Although the component showing the fibroblast proliferation activity contained in the leaves and / or stems of the moon peach has not yet been identified, the components are extracted from the leaves and / or stems of the moon peach by an extraction treatment using a polar solvent as an extraction solvent. It can be easily extracted.

Specific examples of suitable polar solvents include water, lower aliphatic alcohols, and hydrated lower aliphatic alcohols. These may be used alone or in combination of two or more. be able to. Specific examples of suitable lower aliphatic alcohols include methanol, ethanol, propanol, 1,3-butylene glycol, glycerin, propylene glycol and the like.

When a mixture of two or more polar solvents is used as the extraction solvent, the mixing ratio can be adjusted as appropriate. For example, when a mixed solution of water and a lower aliphatic alcohol is used, the mixing ratio of the water and the lower aliphatic alcohol can be 3: 1 to 1: 9 (weight ratio).

As the extraction solvent, it is particularly preferable to use water among the above-mentioned polar solvents. Water that can be used as the extraction solvent includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. Examples of the treatment applied to water include heating,
This includes sterilization, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, water that can be used as the extraction solvent includes hot water, ion-exchanged water, physiological saline, phosphate buffer,
Phosphate buffered saline and the like are also included.

The extraction treatment is not particularly limited as long as the soluble components contained in the leaves and / or stems of the moon peach can be eluted into the extraction solvent, and can be carried out according to a conventional method. In the extraction treatment, it is not necessary to employ a special extraction method, and any device can be used at room temperature or under reflux.

For example, a raw material for extraction is charged into a treatment tank filled with an extraction solvent, and a soluble component can be eluted with occasional stirring. At this time, the amount of the extraction solvent is not particularly limited, and can be appropriately adjusted according to the amount of the extraction raw material.
It is usually 500 to 2000 ml for 100 g. The extraction time is usually 1 to 5 hours, and the extraction temperature is usually 40 to 90 ° C.

After the soluble component is eluted by the extraction treatment, an extraction liquid can be obtained by filtering to remove the extraction residue. The obtained extract is diluted according to a conventional method to obtain a diluent or concentrate of the extract, a dried product of the extract, or a crude product or a purified product thereof.
Treatment such as concentration, drying, and purification may be performed. In obtaining a dried extract, a carrier such as dextrin or cyclodextrin may be added to improve the hygroscopicity.

Since the extract from the leaves and / or stem of the moon peach has a peculiar smell and taste, it may be decolorized and deodorized within a range that does not cause a decrease in its physiological activity.

The extract from the leaves and / or stem of the moon peach obtained as described above has a fibroblast proliferating action. The mechanism of action of the extract from the leaves and / or stems of moon peach has a fibroblast-proliferating action, which has not been elucidated so far, but it will be described later that this extract has a fibroblast-proliferating action. As is apparent from the examples, the agent for promoting fibroblast proliferation of the present invention utilizes the fibroblast proliferation effect of extracts from leaves and / or stems of moon peach.

The extract from the leaves and / or stem of the moon peach can be used as it is as a fibroblast-proliferating agent, but it can also be formulated into a conventional method and used. In the case of formulation, a pharmaceutically acceptable carrier such as dextrin and cyclodextrin and other optional auxiliaries can be added to facilitate storage and handling. The extract from the leaves and / or stems of the moon peach can be made into any dosage form such as a powder or a tablet by formulation.

The fibroblast-proliferating agent of the present invention can increase the production of interstitial substances such as collagen, fibronectin, and mucopolysaccharide by fibroblasts through fibroblast proliferation. In particular, the agent for promoting fibroblast proliferation of the present invention can exert a beautiful skin effect or a skin aging preventing effect by increasing the amount of collagen produced through proliferation of fibroblasts. Therefore, the fibroblast-proliferating agent of the present invention can be used as a raw material for cosmetic foods and drinks and skin cosmetics.

The extract from the leaves and / or stems of the moon peach has a unique odor and taste, but the fibroblast-proliferating agent of the present invention is used as a raw material for cosmetics, foods and drinks and skin cosmetics. When used, they are not generally blended in large quantities, so that there is no practical problem even if they are used without decolorization, deodorization, etc.

[Beauty Foods and Drinks] The beauty foods and drinks of the present invention are characterized by containing an extract from leaves and / or stems of moon peach. Here, "beauty foods and drinks" means foods and drinks for the purpose of preventing beautiful skin or skin from aging.

The extract from the leaves and / or stems of the moon peach can exert a beautiful skin effect or an antiaging effect on the skin through proliferation of fibroblasts, and is therefore suitable for blending into cosmetics foods and drinks. The cosmetic food or drink of the present invention may be obtained by blending an extract from the leaves and / or stem of moon peach with any food or drink that does not hinder its biological activity,
It may be a food or drink mainly containing an extract from moon peach leaves and / or stems.

In the case of producing a food or drink for beauty by blending an extract from the leaves and / or stem of moon peach, for example, sugars such as dextrin and starch; proteins such as gelatin, soy protein, and corn protein; Alanine,
Amino acids such as glutamine and isoleucine; polysaccharides such as cellulose and gum arabic; oils and fats such as soybean oil and medium-chain fatty acid triglycerides; The extract can be formulated in any dosage form.

The thus-prepared moon peach leaves and / or
Alternatively, the extract from the stem can be used as it is as a cosmetic food or drink, but it can also be added to any food or drink to produce a cosmetic food or drink.

The amount of the extract from the leaf and / or stem of the moon peach in the cosmetic food and drink of the present invention is such that the amount of the extract from the leaf and / or stem of the moon peach is about 0.2 per day.
It is preferable to adjust so as to be about 500 mg.

The food or drink to which the extract from the leaves and / or stem of the moon peach can be added is not particularly limited. Specific examples thereof include drinks such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks. Ice cream, ice sorbet, shaved ice and other ice confections; buckwheat, udon, harusame, gyoza skin,
Noodles such as Shumai skin, Chinese noodles, instant noodles; candy such as candy, candy, gum, chocolate, snacks, biscuits, jelly, jam, cream, baked confectionery;
Fish and livestock processed foods such as kamaboko, ham and sausage;
Dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, and other processed fats and oils and processed foods; sauces, sauces and other seasonings; tablets, granules, etc. Health / nutritional supplements in the form of: soups, stews, salads, side dishes, pickles, etc.

[Skin Cosmetic] The skin cosmetic of the present invention is characterized by containing an extract from moon leaf and / or stem. The extract from the leaves and / or stems of the moon peach can exert a skin-beautifying effect or an anti-skin aging effect through proliferation of fibroblasts, and is therefore suitable for blending into skin cosmetics. That is, the extract from the leaves and / or stems of the moon peach can be absorbed from the skin to help prevent aging of the beautiful skin or the skin.

There are no particular restrictions on the skin cosmetics to which the extract from the leaves and / or stem of the moon peach can be added, but specific examples thereof include ointments, creams, emulsions, lotions, packs and the like.

The amount of the extract from the leaves and / or stems of the moon peach in the skin cosmetic of the present invention can be appropriately adjusted depending on the kind of the skin cosmetic, the physiological activity of the extract, and the like. The compounding ratio is about 0.01 to 10% by weight in terms of the dry matter of the unrefined standard extract.

The skin cosmetic of the present invention may contain various main ingredients and auxiliaries usually used in the manufacture of skin cosmetics, as long as it does not hinder the fibroblast proliferation action. The extract from the stem and / or stem is not limited to the main agent.

In the skin cosmetic composition of the present invention, those which can be used as constituents together with extracts from moon leaf and / or stem include, for example, glycerin, collagen, hyaluronic acid and its salts, chondroitic acid and its Moisturizing agents such as salts, chitin and chitosan; ultraviolet absorbers such as amyl paradimethylaminobenzoate; complex lipids such as glycerophospholipids and sphingolipids; β-carotene, oil-soluble licorice extract, lycochalcone A, baicalin,
Baicalein and other substances having an active oxygen scavenging effect;
Azulene, glycyrrhizic acid and its salts, glycyrrhetic acid and its derivatives, anti-inflammatory substances such as zinc oxide; riboflavin, tocopherol, ascorbic acid,
Vitamins such as folic acid and derivatives thereof; oily components such as jojoba oil, lanolin, liquid paraffin, squalane, isostearyl alcohol; surfactants such as sodium stearyl sulfate, diethanolamine cetyl sulfate and glyceryl stearate; oxidation of sodium erythorbate and the like Preservatives such as ethyl paraben; cholesterols such as oak extract, chamomile extract, liquorice root extract, rosemary extract, marronnier extract; plant sterols; lipoproteins; bifidobacterium culture, lactic acid bacteria Microorganism-derived components such as cultures, yeast extracts, and liquorice extracts; algal extracts such as brown algae extracts and red algae extracts; γ-
Blood circulation promoters such as oryzanol; antiseborrheic agents such as sulfur; fragrances; alcohols; thickeners such as carboxy polymers; titanium yellow, safflower and other coloring agents.

The fibroblast-proliferating agent, cosmetics, food and drink, and skin cosmetics of the present invention described above are suitably applied to humans, but the effects of the present invention are exhibited. As long as it is applicable to animals other than humans.

[0040]

EXAMPLES The present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to these examples.

[Preparation Example 1] Leaves and stems of moon peaches were each crushed by a crusher as an extraction raw material, and 300 g of the crushed leaves and stems of peaches were each added to water, 50% ethanol (weight ratio of water to ethanol). 1: 1), methanol and 50% propanol (weight ratio of water to propanol 1: 1) were added in an amount of 2000 ml each, and the mixture was stirred gently for 3 hours while keeping the temperature at 70 ° C., followed by filtration using a filter paper. Extracts from leaves and stems were obtained. The extract was concentrated under reduced pressure at 40 ° C., and then dried with a vacuum drier to obtain a dried extract. Yield of extract obtained using each extraction raw material and each extraction solvent (% by weight)
Was as shown in Table 1 below.

[0042] [Table 1] Sample No. Extraction Solvent month peach leaf extract yield Tsukimomokuki extract yield 1 water 28% 11% 2 50% Ethanol 16% 7% 3 methanol 13% 7% 4 50% Propanol 12% 7%

[Production Example 2] 300 g of the same leaves and stems of the same peach as those used in Production Example 1 were put into 2000 ml of a mixed solution of 1,3-butylene glycol and water (weight ratio 1: 1). After holding at 80 ° C. for 3 hours with stirring, the mixture was filtered to obtain a 4.2% by weight solids extract of moon peach leaves, solids concentration
A 3.2% by weight moon peach stem extract was obtained.

[Preparation Example 3] The same procedure as in Preparation Example 2 was carried out except that the extraction solvent was changed to a mixture of glycerin and water (weight ratio: 1: 1). A moon peach stem extract having a solid content of 2.8% by weight was obtained.

[Preparation Example 4] A month peach leaf extract having a solid content of 3.3% by weight was prepared in the same manner as in Preparation Example 2, except that the extraction solvent was changed to a mixture of propylene glycol and water (weight ratio 1: 1). A moon peach extract with a solid content of 3.0% by weight was obtained.

Test Example 1 The 50% ethanol extract of moon peach leaves and 50% ethanol extract of moon peach stalks obtained in Production Example 1 were tested for their fibroblast proliferation activity by Tada et al. Ta
da et. al., J. Immunol. Methods 93, 157, 1986). The test method is as follows.

(1) Human normal neonatal skin fibroblasts (NB
1RGB) 1 × 10 ⁶ MEM-α-Medium containing 10% FBS
(GIBCO) In a 25 cm ² flask containing a culture solution (pH 7.2), the cells were precultured at 37 ° C under 5% carbon dioxide for 4 days. After the pre-culture, the cells were collected by trypsin treatment, dispensed at 7 × 10 ³ cells / 100 μl / well into a 96-well microplate using a MEM-α-Medium culture solution containing 5% FBS, and the mixture was placed at 37 ° C. Cultured overnight under 5% carbon dioxide.

(2) A 50% ethanol extract of the moon peach leaves obtained in Production Example 1 (sample a) or 50% of the moon peach stem was added to a MEM-α-Medium culture solution containing 5% FBS. Dissolve the ethanol extract (sample b) at various concentrations (100 ppm, 50 ppm)
Add 10 wells to each well of the microplate obtained in (1) above.
After adding 0 μl each, the cells were cultured at 37 ° C. under 5% carbon dioxide for 3 days. After removing 100 μl of culture solution from each well, add MTT reagent (3- (4,5-Dimethyl-2-thiazoly
l) 20 μl of 2,5-diphenyl-2H tetrazolium bromide (manufactured by Dojindo) (5 mg / ml) was added, and the mixture was incubated for 4.5 hours. After the incubation, 100 μl of a 0.01N hydrochloric acid solution in which 10% SDS (sodium lauryl sulfate) (manufactured by Wako Pure Chemical Industries, Ltd.) was dissolved was added to each well, and the mixture was incubated at 37 ° C. overnight. After the incubation, the absorbance at 570 nm was measured using a microplate reader with the absorbance at 650 nm as a control. Then, by subtracting the absorbance at 650 nm from the absorbance at 570 nm, the absorbance at the time of the sample reaction (570 nm-650 nm) was obtained for sample a and sample b.

(3) To each well of the microplate obtained in (1) above, the same operation as in (2) was performed without adding the MTT reagent, and the absorbance at 570 nm was measured using the absorbance at 650 nm as a control. did. And 570
By subtracting the absorbance at 650 nm from the absorbance at nm, the absorbance at the time of no reaction of the sample (570 nm-650 nm) was obtained.

(4) The same operation as in (2) above was performed without adding sample a and sample b to each well of the microplate obtained in (1) above, and the absorbance at 650 nm was used as a control at 570 nm. The absorbance was measured. Then, by subtracting the absorbance at 650 nm from the absorbance at 570 nm, the absorbance at the time of the control reaction (570 nm-650 n
m).

(5) To the wells of the microplate obtained in the above (1), the same operation as in the above (2) was performed without adding the sample a, the sample b and the MTT reagent, and the absorbance at 650 nm was used as a control. The absorbance at 570 nm was measured. Then, by subtracting the absorbance at 650 nm from the absorbance at 570 nm, the absorbance (5
70 nm-650 nm).

(6) Based on the following equation, the increase rate (%) of fibroblasts was determined for samples a and b, and the proliferative effect of samples a and b on fibroblasts was evaluated.

[0053]

[Equation 1] Increase rate (%) = [{(AC)-(BD)}
/ (BD)] x 100

In the above formula, A is the absorbance at the time of the sample reaction.
(570 nm-650 nm) and B is the absorbance (570 nm-650 n
m), C represents the absorbance when the sample did not react (570 nm-650 nm), and D represents the absorbance when the control did not react (570 nm-650 nm).

The increase rates (%) of the fibroblasts of the samples a and b were as shown in Table 2 below. In Table 2, the rate of increase (%) of the fibroblasts is expressed as the mean ± SD (n =
7). In Table 2, “ ^** ” indicates p <0.000
1, " ^* " means p <0.05.

[Table 2] Extract concentration (ppm) Sample a Sample b 100 29.2 ^** ± 6.5 20.6 ^** ± 5.1 50 14.4 ^* ± 8.0 10.2 ^** ± 6.3

As shown in Table 2, both sample a and sample b showed a proliferative effect on fibroblasts. This indicated that the extract from the moon peach leaf and the extract from the moon peach stem had a fibroblast proliferation effect.

Test Example 2 (1) Production of an Emulsion An emulsion having the following composition and containing a 50% ethanol extract of moon peach leaves obtained in Production Example 1 was produced according to a conventional method. 50% ethanol extract of moon peach leaves (Production Example 1) 1.0 g Cetyl alcohol 0.5 g Beeswax 2.0 g Polyoxyethylene sorbitan oleate (10E.O) 1.0 g Glyceryl monostearate 1.0 g Hyaluronic acid 0.1 g Propylene glycol 5.0 g Ethanol 3.0 g Methyl parahydroxybenzoate 0.3 g Fragrance 0.03 g Purified water Remainder (total amount is 100 ml)

(2) Evaluation of Emulsion The following evaluation test was performed on the emulsion a obtained in the above (1) and the emulsion b having the same composition as the emulsion a except that the moon peach extract was not contained. From among a large number of women aged 24 to 43, 20 persons who were judged as “skin rough” which scored 1 or 2 according to the evaluation criteria shown in Table 3 below were selected as subjects. For each subject, latex a was applied to the right half of the face and latex b was applied to the left half of the face.
Was applied once every morning and evening for 30 days.

[Judgment 1: Skin Roughness Improvement Effect] After the application test, a replica of the face skin was taken by a replica method using Silflo (manufactured by FLEXICL DEVELOPMENTS LTD). Was observed, and the condition of the skin was determined according to the following evaluation criteria.

[0061] [Table 3] disappears commentary points Evaluation 1 Kawamizo-skin hill, a wide range of horny layer is turned up. (Rough skin condition) 2 Skin grooves / skins are unclear. The stratum corneum is partially turned up. (Rough skin condition) 3 Skin furrow / skin is observed but it is flat. (Normal skin) 4 Skin groove / skin is clear. (Relatively beautiful skin) 5 The skin sulcus / skin is extremely clear and tidy. (Beautiful skin)

The results of the skin condition determination were as shown in Table 4 below.

[Table 4] Before start of evaluation test After application of emulsion a and after application of emulsion b 1 12 0 8 8 2 8 0 8 3 0 7 7 3 4 0 8 1 1 50 Name 5 people 0 people

As shown in Table 4, when the emulsion a was applied, the skin roughness was remarkably improved as compared with the case where the emulsion b was applied. This indicated that a 50% ethanol extract of moon peach leaves had a skin-beautifying effect or an anti-aging effect on the skin.

[Judgment 2: Sensory evaluation] The subject was asked about the superiority and inferiority of the emulsion a and the emulsion b with respect to the feeling of use and the effect on the skin. The response results are as shown in Table 5 below.

[Table 5] Evaluation items Emulsion a is good. Emulsion b is good . Skin familiarity 13 people 5 people 2 people Moist feeling 19 people 0 people 1 Skin growth 15 people 3 2 Improvement of rough skin Satisfaction 17 2 1 1 Satisfaction of skin color improvement 14 4 2 Improvement of number and depth of wrinkles 19 1 0

As shown in Table 5, when the emulsion a was applied, it was possible to give the user a better feeling of use than the case where the emulsion b was applied, and also to provide an excellent skin effect or skin aging prevention effect. Indicated.

Formulation Example 1 Using the water extract of moon peach leaves obtained in Production Example 1, a soft drink having the following composition was produced by a conventional method. Liquid sugar 12.0g Lemon juice 1.0g Cyclodextrin 0.01g Lemon flavor 0.05g Water extract of moon peach leaves (Production example 1) 0.1g Purified water Remainder (total amount is 100g)

[Formulation Example 2] Using a 50% ethanol extract of the moon peach leaves obtained in Production Example 1, a chewing gum having the following composition was produced by a conventional method. Gum base 20g sugar 55g fructose 15g starch syrup 20g flavor 0.5g 50% ethanol extract of moon peach leaf (Production Example 1) 0.5g

[Formulation Example 3] Using the 50% ethanol extract of the moon peach stalk obtained in Production Example 1, tablets having the following composition were produced by a conventional method. 50% ethanol extract of moon peach stem (Production Example 1) 50 mg Powdered sugar (sucrose) 188 mg Glycerin fatty acid ester 12 mg

[Formulation Example 4] Using the water extract of the moon peach stalk obtained in Production Example 1, granules having the following composition were produced by a conventional method. Water peach stem extract 34mg Beet oligosaccharide 1000mg Indigestible dextrin 289mg Vitamin C 167mg Stevia extract 10mg

Formulation Example 5 Using the 50% ethanol extract of the moon peach leaves obtained in Production Example 1 and the 50% glycerin extract of the moon peach leaves obtained in Production Example 3, the following composition was used: Was prepared by a conventional method. Jojoba oil 4.0 g Olive oil 2.0 g Squalane 2.0 g Cetanol 2.0 g Glyceryl monostearate 2.0 g Polyoxyethylene cetyl ether (20E.0) 2.5 g Polyoxyethylene sorbitan oleate (20E.0) 2.0 g 1,3-butylene Glycol 3.0 g Methyl parahydroxybenzoate 0.15 g Flavor 0.05 g 50% ethanol extract of moon peach leaves (Production Example 1) 0.1 g 50% glycerin extract of moon peach leaves (Production Example 3) 1.0 g Purified water remainder ( The total amount is 100 g)

[Formulation Example 6] Using the water extract of the moon peach leaves obtained in Production Example 1 and the 50% glycerin extract of the moon peach stem obtained in Production Example 3, a makeup having the following composition Water was produced in a conventional manner. Glycerin 3.0 g 1,3-butylene glycol 3.0 g Polyoxyethylene sorbitan oleate (20E.0) 0.5 g Methyl parahydroxybenzoate 0.15 g Citric acid 0.1 g Sodium citrate 1.0 g Flavor 0.05 g Water extract of moon peach leaves (Production Example 1) 0.2 g 50% glycerin extract of moon peach stalk (Production Example 3) 2.0 g Purified water balance (total amount is 100 g)

[Formulation Example 7] Using 50% ethanol extract of moon peach leaves obtained in Production Example 1 and 50% 1,3-butylene glycol extract of moon peach leaves obtained in Production Example 2 hand,
A cream having the following composition was produced by a conventional method. Liquid paraffin 5.0 g Salamander wax 4.0 g Cetanol 3.0 g Squalane 10.0 g Lanolin 2.0 g Stearic acid 1.0 g Polyoxyethylene sorbitan oleate (20E.0) 1.5 g Glyceryl monostearate 3.0 g 1,3-butylene glycol 6.0 g Paraoxybenzoate Methyl acrylate 1.5g Flavor 0.1g 50% ethanol extract of moon peach leaves (Production example 1) 0.1g 50% 1,3-butylene glycol extract of moon peach leaves (Production example 2) 1.0g Purified water The total amount is 100 g)

[Formulation Example 8] Using the 50% propylene glycol extract of moon peach leaves obtained in Production Example 4, a pack having the following composition was produced by a conventional method. Polyvinyl alcohol 15g Polyethylene glycol 3g Propylene glycol 7g Ethanol 10g Ethyl parahydroxybenzoate 0.05g Fragrance 0.05g 50% propylene glycol extract of moon peach leaves (Production example 4) 5g Purified water The remainder (total amount is 100g)

[Formulation Example 9] Using the 50% ethanol extract of the leaves and stems of the moon peach obtained in Production Example 1, a mixture having the following composition was prepared, and the mixture was formed into granules for health.・
Dietary supplements were manufactured. 50% ethanol extract of moon peach leaves and stem (Production Example 1) 30 parts by weight Lotus embryo extract 200 parts by weight Pearl protein hydrolyzate (including various amino acids and peptides) 15 parts by weight Silk protein hydrolyzate (various types) 15 parts by weight of soy isoflavones 10 parts by weight Red wine extract powder (including procyanidins) 30 parts by weight Yeast extract (including glutathione, nucleic acids, amino acids and vitamins) 10 parts by weight Pomegranate seed extract 10 parts by weight Wheat germ Extract (including vitamins, chromium, selenium, molybdenum) 10 parts by weight DHA 30 parts by weight Beet oligosaccharide 1060 parts by weight Stevia extract 10 parts by weight

[Formulation Example 10] Using the water extract of the leaves and stems of the moon peach obtained in Production Example 1, a mixture having the following composition was prepared, and the mixture was gelatin-encapsulated to obtain a tablet-like health and nutrition. Supplementary food was manufactured. Water extract of moon peach leaves and stems (Production Example 1) 25 parts by weight Lotus germ extract 20 parts by weight Ceramide 25 parts by weight Phospholipid (lecithin) 10 parts by weight Vitamin E (tocopherol) 17 parts by weight Multi-carotene (α and Beta-carotene, lutein, lycopene) 10 parts by weight Octacosanol 1 part by weight Plant sterol 5 parts by weight Perilla oil (including α-linolenic acid) 20 parts by weight Refined fish oil (including DHA and EPA) 20 parts by weight Sesame oil (with lignan compound) 20 parts by weight Olive oil 10 parts by weight Soybean and rapeseed mixed oil 62 parts by weight Glycerin fatty acid ester 12 parts by weight

[Blending Example 11] Using the 50% glycerin extract of the leaves and stems of the moon peach obtained in Production Example 3, a soft drink having the following composition was produced by a conventional method. 50% glycerin extract of moon peach leaves and stem (Preparation Example 3) 1 part by weight Lotus germ extract 1 part by weight Royal jelly 3 parts by weight Water-soluble collagen 8 parts by weight Barley extract 1 part by weight Korean ginseng extract (including saponin) 1 part by weight Placenta (placenta) extract 1 part by weight Pueraria extract (including flavonoids) 1 part by weight Purple yam extract 1 part by weight Oligosaccharide 5 parts by weight Sucrose 10 parts by weight Prune juice 2 parts by weight Pomegranate juice 5 parts by weight Grapefruit juice 10 Parts by weight Vitamin C 1 part by weight Grapefruit flavor 0.7 parts by weight Water Remainder (total amount is 100 parts by weight)

[Formulation Example 12] Using the 50% ethanol extract of the leaves and stems of the moon peach obtained in Production Example 1, a candy having the following composition was produced by a conventional method. 5% by weight ethanol extract of moon peach leaves and stem (Production Example 1) 5 parts by weight Lotus germ extract 2 parts by weight Mizuame 30 parts by weight Licorice extract 3 parts by weight Tencha extract 1 part by weight Blueberry extract (including anthocyanin) 1 part by weight Part Green tea extract (including plant polyphenol) 3 parts by weight Strawberry 1/5 concentrated juice 1 part by weight Red cabbage pigment 0.05 parts by weight Lemon juice 0.5 parts by weight Water 5 parts by weight

[0080]

Industrial Applicability According to the present invention, a fibroblast-proliferating agent capable of proliferating fibroblasts, a cosmetic food or drink capable of preventing aging of beautiful skin or skin through proliferation of fibroblasts, and A skin cosmetic is provided.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A23L 2/52 A23L 2/38 C 2/38 A61K 7/00 K A61K 7/00 W 7/48 7 / 48 A23L 2/00 FF term (reference) 4B014 GB08 GB13 GG18 GK06 4B017 LC03 LG15 LP01 4B018 MD61 ME10 MF01 4C083 AA032 AA082 AA111 AA112 AA122 AB052 AC022 AC072 AC102 AC122 AC242 AC252 AC392 AC402 AC482 AD212 AD212 AD212 AD212 AD212 DD23 DD27 DD31 EE12 4C088 AB81 AC05 CA04 CA05 CA06 NA14 ZA89 ZB21

Claims (5)

[Claims] 1. A fibroblast-proliferating agent comprising, as an active ingredient, an extract from leaves and / or stems of moon peach. 2. The agent according to claim 1, wherein the extract is an extract obtained using a polar solvent as an extraction solvent. 3. The polar solvent is water, methanol, ethanol, propanol, 1,3-butylene glycol,
3. The agent according to claim 2, which is glycerin, propylene glycol, or a mixture of two or more thereof. 4. A cosmetic food or drink comprising an extract from leaves and / or stems of moon peach. 5. A skin cosmetic comprising an extract from moon peach leaves and / or stem.