JP2001272398A - Specimen analyzing tool and container used for the same - Google Patents
Specimen analyzing tool and container used for the sameInfo
- Publication number
- JP2001272398A JP2001272398A JP2000084352A JP2000084352A JP2001272398A JP 2001272398 A JP2001272398 A JP 2001272398A JP 2000084352 A JP2000084352 A JP 2000084352A JP 2000084352 A JP2000084352 A JP 2000084352A JP 2001272398 A JP2001272398 A JP 2001272398A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- porous sheet
- container
- analysis tool
- sample analysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000011800 void material Substances 0.000 claims abstract description 8
- 238000004458 analytical method Methods 0.000 claims description 36
- 239000007788 liquid Substances 0.000 claims description 7
- 210000004369 blood Anatomy 0.000 claims description 6
- 239000008280 blood Substances 0.000 claims description 6
- 239000011347 resin Substances 0.000 claims description 4
- 229920005989 resin Polymers 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 description 16
- 229920000139 polyethylene terephthalate Polymers 0.000 description 7
- 239000005020 polyethylene terephthalate Substances 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 239000004800 polyvinyl chloride Substances 0.000 description 5
- 229920000915 polyvinyl chloride Polymers 0.000 description 5
- 229920000122 acrylonitrile butadiene styrene Polymers 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 229920000178 Acrylic resin Polymers 0.000 description 3
- 239000004925 Acrylic resin Substances 0.000 description 3
- 239000012491 analyte Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000006866 deterioration Effects 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 229920002492 poly(sulfone) Polymers 0.000 description 3
- 229920000728 polyester Polymers 0.000 description 3
- -1 polyethylene terephthalate Polymers 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 125000006850 spacer group Chemical group 0.000 description 3
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
- HVBSAKJJOYLTQU-UHFFFAOYSA-N 4-aminobenzenesulfonic acid Chemical compound NC1=CC=C(S(O)(=O)=O)C=C1 HVBSAKJJOYLTQU-UHFFFAOYSA-N 0.000 description 2
- FRPHFZCDPYBUAU-UHFFFAOYSA-N Bromocresolgreen Chemical compound CC1=C(Br)C(O)=C(Br)C=C1C1(C=2C(=C(Br)C(O)=C(Br)C=2)C)C2=CC=CC=C2S(=O)(=O)O1 FRPHFZCDPYBUAU-UHFFFAOYSA-N 0.000 description 2
- 108010015776 Glucose oxidase Proteins 0.000 description 2
- 239000004366 Glucose oxidase Substances 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 238000008050 Total Bilirubin Reagent Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229940116332 glucose oxidase Drugs 0.000 description 2
- 235000019420 glucose oxidase Nutrition 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- HEBKCHPVOIAQTA-ZXFHETKHSA-N ribitol Chemical compound OC[C@H](O)[C@H](O)[C@H](O)CO HEBKCHPVOIAQTA-ZXFHETKHSA-N 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000008279 sol Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 229950000244 sulfanilic acid Drugs 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、多孔性シートを容
器内に収納した検体分析用具に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a device for analyzing a sample in which a porous sheet is contained in a container.
【０００２】[0002]
【従来の技術】臨床医療等の分野において、一回の検査
毎に使い切る検体分析用具が汎用されている。例えば、
ろ紙などの多孔性シートを、樹脂製容器に収納した検体
分析用具では、前記ろ紙の一部に血液等の検体を点着す
ると、これが、毛細管現象により、前記ろ紙中を展開す
る。例えば、全血の場合、この展開の際に、クロマトグ
ラフィー効果により、血球と血漿・血清が分離される。
そして、多孔性シートに分析試薬等が保持されている場
合は、これと検体内の成分とが反応し、光学的手段若し
くは電気化学的手段で分析が行われる。また、多孔性シ
ート自身に分析試薬が保持されていない場合は、この検
体分析用具は、検体の保持若しくは保存に使用され、こ
の場合は、液状検体が乾燥した状態で、保持若しくは保
存される。そして、サンプリングから一定時間経過後、
検体分析用具から、多孔性シートを取り出し、これから
血漿や血清を抽出して、分析にかける。2. Description of the Related Art In the field of clinical medicine and the like, a sample analyzing tool used up for each test is widely used. For example,
In a sample analysis tool in which a porous sheet such as filter paper is stored in a resin container, when a sample such as blood is spotted on a part of the filter paper, the sample spreads through the filter paper due to a capillary phenomenon. For example, in the case of whole blood, at the time of this development, blood cells and plasma / serum are separated by a chromatographic effect.
When an analytical reagent or the like is held on the porous sheet, this reacts with a component in the specimen, and analysis is performed by optical means or electrochemical means. When the porous sheet itself does not hold the analysis reagent, the sample analysis tool is used for holding or storing the sample. In this case, the liquid sample is held or stored in a dry state. Then, after a certain time has passed since sampling,
The porous sheet is taken out from the sample analysis tool, and plasma and serum are extracted therefrom and subjected to analysis.
【０００３】[0003]
【発明が解決しようとする課題】このような検体分析用
具では、液状検体が、多孔性シート内部ではなく、これ
と容器内壁との間に浸透するという現象が起こることが
あった。このように浸透した液状検体は、クロマトグラ
フィー効果により分離されておらず、これにより、多孔
性シート内で分離した成分が汚染され、分析に影響を与
えるおそれがある。この問題を解決する手段として、多
孔性シートの検体展開部分を充分に大きくとる方法が考
えられるが、これでは、検体分析用具が、大きくなりす
ぎ、操作が不便になり、またコスト的にも不利になる。In such a sample analysis device, a phenomenon that the liquid sample permeates between the porous sheet and the inner wall of the container instead of the inside thereof may occur. The liquid sample that has penetrated in this way is not separated by the chromatographic effect, which may contaminate the components separated in the porous sheet and affect the analysis. As a means for solving this problem, a method in which the sample development portion of the porous sheet is made sufficiently large is conceivable, but in this case, the sample analysis tool becomes too large, the operation becomes inconvenient, and the cost is disadvantageous. become.
【０００４】したがって、本発明の目的は、多孔性シー
トが容器内部に収納された検体分析用具であって、前記
多孔性シートと前記容器内壁との間に液状検体が浸透す
ることが防止される検体分析用具を提供することであ
る。Accordingly, an object of the present invention is a sample analysis tool in which a porous sheet is accommodated in a container, wherein a liquid sample is prevented from penetrating between the porous sheet and the inner wall of the container. It is to provide a sample analysis tool.
【０００５】[0005]
【課題を解決するための手段】前記目的を達成するため
に、本発明の検体分析用具は、多孔性シートが容器内部
に収納された検体分析用具であって、前記多孔性シート
が、検体供給部および毛細管現象により検体を展開する
検体展開部を有し、前記多孔性シートの前記検体供給部
と前記検体展開部との間の部分と前記容器内壁との間
に、毛細管現象が発生しない大きさの空隙が形成されて
いることを特徴とする。According to the present invention, there is provided a sample analyzing tool comprising a porous sheet housed in a container, wherein the porous sheet comprises a sample supplying device. And a sample developing section for expanding the sample by capillary action, and a size that does not cause capillary action between the portion of the porous sheet between the sample supply section and the sample developing section and the inner wall of the container. It is characterized in that an air gap is formed.
【０００６】本発明者が、多孔性シートと容器内壁との
間の検体の浸透について検討したところ、これは毛細管
現象が発生していることが原因であることを突き止め
た。したがって、本発明の検体分析用具では、この毛細
管現象が発生しないような空隙を、多孔性シートの検体
供給部と検体展開部との間の部分（以下「境界部分」と
もいう）と前記内壁との問に設けることにより、検体の
浸透を防止できる。[0006] The present inventor examined the penetration of the sample between the porous sheet and the inner wall of the container, and found out that this was caused by the occurrence of capillary action. Therefore, in the sample analysis device of the present invention, a gap where the capillary phenomenon does not occur is formed between the portion between the sample supply section and the sample development section of the porous sheet (hereinafter also referred to as “boundary section”) and the inner wall. In this case, the penetration of the sample can be prevented.
【０００７】本発明にかかる前記多孔性シートにおい
て、多孔性シート内の検体の流れを基準にすると、前記
検体供給部とは、前記境界部分より上流側の部分の一部
もしくは全部をいい、前記検体展開部とは、前記境界部
分より下流側の部分の一部若しくは全部をいう。また、
本発明において、検体は、毛細管現象により、多孔性シ
ート内部を移動することが可能なものをいい、液状検体
に限定されず、例えば、ゾル状の検体であってもよい。
本発明に適用できる検体としては、例えば、全血、血
漿、血清、尿、骨髄液、唾液、分泌物、その他体液等が
あげられる。In the porous sheet according to the present invention, with reference to the flow of the sample in the porous sheet, the sample supply section refers to a part or all of a portion on the upstream side of the boundary portion. The sample developing unit refers to a part or the whole of a part downstream from the boundary part. Also,
In the present invention, the sample refers to a sample that can move inside the porous sheet due to capillary action, and is not limited to a liquid sample, and may be, for example, a sol sample.
Samples applicable to the present invention include, for example, whole blood, plasma, serum, urine, bone marrow fluid, saliva, secretions, and other body fluids.
【０００８】本発明の検体分析用具において、例えば、
前記容器内部に突起状支持部が形成され、この支持部に
より前記多孔性シートの検体供給部と検体展開部との間
の部分が持ち上げられることにより空隙が形成される。
また、前記支持部と反対側の容器内部に突起状押え部が
形成され、この押え部により前記多孔性シートの検体展
開部が、容器内壁に固定されていることが好ましい。こ
のようにすれば、多孔性シート全体もしっかりと固定さ
れるからである。In the sample analysis device of the present invention, for example,
A projecting support portion is formed inside the container, and a space is formed by lifting a portion of the porous sheet between the sample supply portion and the sample development portion by the support portion.
Further, it is preferable that a protruding holding portion is formed inside the container on the side opposite to the support portion, and the sample developing portion of the porous sheet is fixed to the inner wall of the container by the holding portion. By doing so, the entire porous sheet is also firmly fixed.
【０００９】前記空隙は、毛細管現象が発生しない大き
さであれば、特に制限されず、検体の粘性（若しくは表
面張力）、容器内壁および多孔性シートの材質などの条
件により適宜決定される。例えば、前記検体が血液（全
血）である場合は、前記空隙の最大幅は、例えば、０．
１〜３ｍｍの範囲、好ましくは０．２〜２ｍｍの範囲、
特に好ましくは０．３〜０．７ｍｍの範囲である。The space is not particularly limited as long as it does not cause capillary action, and is appropriately determined depending on conditions such as the viscosity (or surface tension) of the specimen, the material of the container inner wall and the porous sheet. For example, when the sample is blood (whole blood), the maximum width of the void is, for example, 0.
In the range of 1 to 3 mm, preferably in the range of 0.2 to 2 mm,
Particularly preferably, it is in the range of 0.3 to 0.7 mm.
【００１０】前記多孔性シートは、例えば、ろ紙、樹脂
シート等があげられる。樹脂シートとしては、例えば、
ポリエステル、ポリスルホン、ポリカーボネート、セル
ロースアセテート等の材質製のシートがあげられる。ま
た、前記多孔性シートは、その平均孔径が、シート厚み
方向に添って連続的若しくは段階的に変化する非対称多
孔性シートであってもよい。これらの多孔性シートは、
単独で使用してもよいし、これらを２種類以上組み合わ
せて使用してもよい。好ましい多孔性シートは、ろ紙、
ポリスルホン製多孔性シート、ポリエステル製多孔性シ
ート、ポリカーボネート製多孔性シートであり、より好
ましい多孔性シートは、ろ紙、ポリスルホン製多孔性シ
ート、ポリエステル製多孔性シートである。多孔性シー
トの平均孔径は、例えば、１〜５００μｍであり、好ま
しくは２〜１００μｍであり、より好ましくは５〜５０
μｍである。多孔性シートの平均厚みは、例えば、１０
〜２０００μｍであり、好ましくは１００〜１０００μ
ｍであり、より好ましくは３００〜５００μｍである。
多孔性シートの大きさは、容器内部の大きさにより適宜
検定されるが、長方形状の場合、例えば、２２×２２〜
２×２５０ｍｍ、好ましくは２０×２５〜３×１６７ｍ
ｍ、より好ましくは５×１００ｍｍである。[0010] Examples of the porous sheet include a filter paper and a resin sheet. As a resin sheet, for example,
Examples include sheets made of a material such as polyester, polysulfone, polycarbonate, and cellulose acetate. Further, the porous sheet may be an asymmetric porous sheet whose average pore diameter changes continuously or stepwise along the sheet thickness direction. These porous sheets are
They may be used alone or in combination of two or more. Preferred porous sheets are filter paper,
A porous sheet made of polysulfone, a porous sheet made of polyester, and a porous sheet made of polycarbonate are preferred. More preferred porous sheets are filter paper, a porous sheet made of polysulfone, and a porous sheet made of polyester. The average pore size of the porous sheet is, for example, 1 to 500 μm, preferably 2 to 100 μm, more preferably 5 to 50 μm.
μm. The average thickness of the porous sheet is, for example, 10
２０００2000 μm, preferably 100-1000 μm
m, more preferably 300 to 500 μm.
The size of the porous sheet is appropriately determined depending on the size of the inside of the container. In the case of a rectangular shape, for example, 22 × 22 to
2 x 250 mm, preferably 20 x 25 to 3 x 167 m
m, more preferably 5 × 100 mm.
【００１１】前記容器の材質は、例えば、ＰＥＴ（ポリ
エチレンテレフタレート）、ＰＶＣ（ポリ塩化ビニ
ル）、ＡＢＳ樹脂、ＰＰ（ポリプロピレン）、アクリル
樹脂等があげられ、好ましくはＰＥＴ，ＡＢＳ樹脂、Ｐ
Ｐであり、より好ましくはＰＥＴ，ＡＢＳ樹脂である。
また、前記容器の検体展開部に対応する部分の一部が透
明であることが好ましい。透明であれば、検体の展開が
外部より目視で確認できるからである。なお、一部透明
には、全部透明も含む。透明な材質としては、例えば、
アクリル樹脂、ＰＥＴ、ＰＶＣ、ＡＢＳ樹脂等があり、
好ましくは、ＰＥＴ、ＰＶＣ、アクリル樹脂であり、よ
り好ましくは、ＰＥＴ、ＰＶＣである。The material of the container is, for example, PET (polyethylene terephthalate), PVC (polyvinyl chloride), ABS resin, PP (polypropylene), acrylic resin, etc., preferably PET, ABS resin, P
P, more preferably PET or ABS resin.
Further, it is preferable that a part of a portion of the container corresponding to the sample developing section is transparent. This is because if the sample is transparent, the development of the sample can be visually confirmed from the outside. In addition, partially transparent includes all transparent. As a transparent material, for example,
There are acrylic resin, PET, PVC, ABS resin, etc.
Preferably, they are PET, PVC, and an acrylic resin, and more preferably, PET and PVC.
【００１２】つぎに、本発明の容器は、前記本発明の検
体分析用具に用いる容器であって、検体供給部および毛
細管現象により液状検体を展開させる検体展開部を有す
る多孔性シートを保持するための容器であり、前記多孔
性シートの前記検体供給部および前記検体展開部の間の
部分を持ち上げて、毛細管現象が発生しない大きさの空
隙を形成するための突起状支持部が容器内部に形成され
ている容器である。前述と同様の理由により、前記支持
部と反対側の容器内部に、多孔性シートを容器内壁に固
定するための突起状押え部が形成されていることが好ま
しく、前記多孔性シートの展開部に対応する部分の一部
が透明であるのも好ましい。Next, the container of the present invention is a container used for the sample analysis tool of the present invention, for holding a porous sheet having a sample supply section and a sample developing section for developing a liquid sample by capillary action. A protruding support portion for raising a portion of the porous sheet between the sample supply unit and the sample development unit to form a void having a size that does not cause capillary action is formed inside the container. Container. For the same reason as described above, it is preferable that a protruding pressing portion for fixing the porous sheet to the inner wall of the container is formed inside the container on the side opposite to the support portion, and the developing portion of the porous sheet is It is also preferred that some of the corresponding parts are transparent.
【００１３】本発明において、前記容器の大きさは特に
制限されないが、例えば、全体の大きさ５ｍｍ×３０ｍ
ｍ〜５０ｍｍ×８０ｍｍ、全体厚み０．５〜１０ｍｍ、
突起状支持部の高さ０．１〜２．０ｍｍ、突起状押え部
の高さ０．１〜５．０ｍｍである。好ましくは、全体の
大きさ５ｍｍ×４０ｍｍ〜４０ｍｍ×７０ｍｍ、全体厚
み０．５〜５．０ｍｍ、前記突起状支持部の高さ０．２
〜１．０ｍｍ、突起状押え部の高さ０．３〜２．０ｍｍ
である。より好ましくは、全体の大きさ１０ｍｍ×５０
ｍｍ〜３０ｍｍ×６０ｍｍ、全体厚み１．０〜３．０ｍ
ｍ、前記突起状支持部の高さ０．３〜０．７ｍｍ、突起
状押え部の高さ０．４〜１．０ｍｍである。In the present invention, the size of the container is not particularly limited. For example, the entire size is 5 mm × 30 m.
m ~ 50mm x 80mm, overall thickness 0.5 ~ 10mm,
The height of the protruding support portion is 0.1 to 2.0 mm, and the height of the protruding holding portion is 0.1 to 5.0 mm. Preferably, the overall size is 5 mm × 40 mm to 40 mm × 70 mm, the overall thickness is 0.5 to 5.0 mm, and the height of the protruding support portion is 0.2.
~ 1.0mm, height of protruding presser part 0.3 ~ 2.0mm
It is. More preferably, the overall size is 10 mm x 50
mm to 30 mm x 60 mm, overall thickness 1.0 to 3.0 m
m, the height of the protruding support portion is 0.3 to 0.7 mm, and the height of the protruding holding portion is 0.4 to 1.0 mm.
【００１４】[0014]
【発明の実施の形態】図１および図２に、本発明の検体
分析用具の一例を示す。図１（Ａ）は、平面図であり、
同図（Ｂ）は、裏面図であり、同図（Ｃ）は、前記平面
図のＩ−Ｉ方向に見た場合の断面図である。図２は斜視
図である。前記面図において、同一部分には同一符号を
付している。FIG. 1 and FIG. 2 show an example of a sample analysis device according to the present invention. FIG. 1A is a plan view,
FIG. 1B is a rear view, and FIG. 1C is a cross-sectional view when viewed in the II direction of the plan view. FIG. 2 is a perspective view. In the plan view, the same portions are denoted by the same reference numerals.
【００１５】図示のように、この検体分析用具１では、
長方形状の下基板９ｂの縁部にスペーサー４が配置さ
れ、この上に、長方形状の上基板９ａが配置されて容器
が構成されており、この内部に長方形状の多孔性シート
２が収納されている。前記両基板９ａ、９ｂは透明であ
り、これらの縁部の一部にはスペーサー４が配置されて
おらず、この空間が空気抜き部６となる。上基板９ａの
一方の端部には、検体供給用の孔３が形成されており、
多孔性シート２の孔３に対応する部分が検体供給部とな
る。上基板９ａの内面において、孔３周辺に直方体状の
押え部材７が形成されており、この押え部材７にも前記
孔３と連通する孔が形成され、これが検体ガイドの役割
を果たす。また、下基板９の内面において、前記孔３に
対応する部分より長辺方向の中心よりに突起状の支持部
８が形成され、これにより多孔性シート２の一部が持ち
上げられて下基板９ｂ内面（内壁）と多孔性シート２と
の間に空隙が形成されている。多孔性シート２におい
て、前記空隙に対応する部分が境界部分となり、これを
基準として、前記検体供給部と反対側が検体展開部とな
る。この検体展開部は、上基板９ａ内面に形成された２
つの直方体状の押え部材５により、その短辺方向両端が
下基板９ｂ内壁に固定されている。このように、本発明
では、多孔性シートの検体展開部全体を押え部で押さえ
るのではなく、その縁部を押さえるのが好ましい。As shown in the figure, in this sample analysis tool 1,
The spacer 4 is arranged on the edge of the rectangular lower substrate 9b, and the rectangular upper substrate 9a is arranged thereon to form a container, in which the rectangular porous sheet 2 is housed. ing. The substrates 9a and 9b are transparent, and the spacers 4 are not disposed on some of the edges thereof. A hole 3 for supplying a sample is formed at one end of the upper substrate 9a,
A portion corresponding to the hole 3 of the porous sheet 2 becomes a sample supply unit. On the inner surface of the upper substrate 9a, a rectangular parallelepiped pressing member 7 is formed around the hole 3, and a hole communicating with the hole 3 is also formed in the pressing member 7, which functions as a sample guide. Further, on the inner surface of the lower substrate 9, a protruding supporting portion 8 is formed from a portion corresponding to the hole 3 in the center in the long side direction, whereby a part of the porous sheet 2 is lifted and the lower substrate 9 b A void is formed between the inner surface (inner wall) and the porous sheet 2. In the porous sheet 2, a portion corresponding to the void is a boundary portion, and a side opposite to the sample supply section is a sample developing section based on the boundary portion. This sample developing section is formed on the inner surface of the upper substrate 9a.
The two short sides in the short side direction are fixed to the inner wall of the lower substrate 9b by the two rectangular pressing members 5. As described above, in the present invention, it is preferable that the edge portion of the porous sheet is not held by the holding portion but the edge portion thereof is held.
【００１６】この検体分析用具１において、孔３を通し
て多孔性シート２の検体供給部に検体を点着すると、前
記検体は、毛細管現象により、多孔性シート内部を移動
し、展開部で展開する。図１（Ｃ）において、矢印は、
検体の移動方向を示す。この検体分析用具１では、空気
抜き孔６があるため、検体は速やかに展開する。検体
は、多孔性シート２の厚み方向にも移動し、下基板９ｂ
の内面とも接触するが、多孔性シート２の境界部分と下
基板９ｂ内面との間に毛細管現象が発生しない大きさの
空隙が形成されているため、多孔性シート２と下基板９
ｂ内面との間を浸透して、多孔性シート２の検体展開部
まで移動することがない。In the sample analysis device 1, when a sample is spotted on the sample supply section of the porous sheet 2 through the hole 3, the sample moves inside the porous sheet by capillary action and is developed in the developing section. In FIG. 1C, the arrow indicates
Indicates the direction of movement of the sample. In the sample analysis tool 1, the sample is quickly developed because of the air vent hole 6. The sample also moves in the thickness direction of the porous sheet 2, and the lower substrate 9b
However, since a gap is formed between the boundary portion of the porous sheet 2 and the inner surface of the lower substrate 9b so as not to cause a capillary phenomenon, the porous sheet 2 and the lower substrate 9
b. It does not penetrate between the inner surface and move to the sample developing portion of the porous sheet 2.
【００１７】そして、前記展開部に分析試薬等が含浸さ
せてある場合は、これと検体中の成分とが反応し、これ
を光学的手法若しくは電気化学的手法により測定する。
含浸させる分析試薬としては、分析対象成分が糖の場
合、例えば、グルコースオキシダーゼ（ＧＯＤ）および
４−アミノアンチピリンが使用でき、分析対象成分がア
ルブミン（Ａｌｂ）の場合、例えば、ＢＣＧ（ブロムク
レゾールグリーン）が使用でき、分析対象が総ビリルビ
ン（Ｔ−Ｂｉｌ）場合、例えば、スルファニル酸および
亜硝酸が使用できる。When the developing section is impregnated with an analytical reagent or the like, the reacting component reacts with a component in the specimen, and this is measured by an optical method or an electrochemical method.
As the analysis reagent to be impregnated, when the analyte is a sugar, for example, glucose oxidase (GOD) and 4-aminoantipyrine can be used. When the analyte is albumin (Alb), for example, BCG (bromcresol green) When the analyte is total bilirubin (T-Bil), for example, sulfanilic acid and nitrous acid can be used.
【００１８】前記検体展開部に分析試薬がない場合は、
この検体分析用具は、検体の一時的な保持用具若しくは
長期間の保存用具として使用される。そして、検査の際
に、検体分析用具から多孔性シートの検体展開部を取り
出し、これから検体を抽出して検査を行う。このような
使用方法においては、検体は、乾燥した状態で保持若し
くは保存される場合がある。また、このような使用方法
において、多孔性シートの検体展開部に、検体中の成分
の変質などを防止するための物質を保持させておくこと
が好ましい。このような変質防止剤としては、例えば、
シュークロース、トレハロース、アドニトール等の糖
類、クエン酸等がある。When there is no analysis reagent in the sample developing section,
This sample analysis tool is used as a temporary holding tool or a long-term storage tool for a sample. Then, at the time of the test, the sample developing section of the porous sheet is taken out from the sample analysis tool, and the sample is extracted therefrom for the test. In such a method of use, the specimen may be held or stored in a dry state. Further, in such a method of use, it is preferable that a substance for preventing deterioration of components in the specimen is held in the specimen developing section of the porous sheet. As such a deterioration preventing agent, for example,
There are sugars such as sucrose, trehalose and adonitol, and citric acid.
【００１９】前記多孔性シートの検体展開部への分析試
薬や変質防止剤の保持は、例えば、これらの溶液を調製
し、これを前記展開部に塗布や浸漬等により含浸させ、
その後乾燥させることにより実施できる。The holding of the analysis reagent and the deterioration preventing agent in the sample developing portion of the porous sheet is performed, for example, by preparing a solution thereof and impregnating the developing portion with the solution by applying or dipping the solution.
Thereafter, it can be carried out by drying.
【００２０】この検体分析用具１の大きさは、例えば、
収納する多孔性シートの大きさ等に応じて適宜決定でき
るが、例えば、全体の大きさ５ｍｍ×３０ｍｍ〜５０ｍ
ｍ×８０ｍｍ、全体厚み０．５〜１０ｍｍ、孔３の直径
３．０〜２０ｍｍ、押え部材５の高さ０．１〜２．０ｍ
ｍ、孔３周辺の押え部材７の高さ０．１〜５．０ｍｍ、
下基板９ｂの支持部８の高さ０．１〜２．０ｍｍであ
る。好ましくは、全体の大きさ５ｍｍ×４０ｍｍ〜４０
ｍｍ×７０ｍｍ、全体厚み０．５〜５．０ｍｍ、孔３の
直径４．０〜１０ｍｍ、押え部材５の高さ０．３〜１．
０ｍｍ、孔３周辺の押え部材７の高さ０．３〜２．０ｍ
ｍ、下基板９ｂの支持部８の高さ０．２〜１．０ｍｍで
ある。より好ましくは、全体の大きさ１０ｍｍ×５０ｍ
ｍ〜３０ｍｍ×６０ｍｍ、全体厚み１．０〜３．０ｍ
ｍ、孔３の直径５．０〜７．０ｍｍ、押え部材５の高さ
０．４〜０．８ｍｍ、孔３周辺の押え部材７の高さ０．
５〜１．０ｍｍ、下基板９ｂの支持部８の高さ０．３〜
０．７ｍｍである。The size of the sample analysis device 1 is, for example,
It can be appropriately determined according to the size of the porous sheet to be stored, for example, for example, the overall size is 5 mm × 30 mm to 50 m
mx 80 mm, overall thickness 0.5 to 10 mm, diameter of hole 3 3.0 to 20 mm, height of holding member 5 0.1 to 2.0 m
m, the height of the holding member 7 around the hole 3 is 0.1 to 5.0 mm,
The height of the support portion 8 of the lower substrate 9b is 0.1 to 2.0 mm. Preferably, the overall size is 5 mm × 40 mm to 40 mm
mm × 70 mm, overall thickness 0.5 to 5.0 mm, diameter of hole 3 4.0 to 10 mm, height of holding member 5 0.3 to 1.
0 mm, height of holding member 7 around hole 3 0.3 to 2.0 m
m, the height of the supporting portion 8 of the lower substrate 9b is 0.2 to 1.0 mm. More preferably, the overall size is 10 mm x 50 m
m ~ 30mm x 60mm, overall thickness 1.0 ~ 3.0m
m, the diameter of the hole 3 is 5.0 to 7.0 mm, the height of the pressing member 5 is 0.4 to 0.8 mm, and the height of the pressing member 7 around the hole 3 is 0.
5 to 1.0 mm, the height of the support portion 8 of the lower substrate 9b is 0.3 to
0.7 mm.
【００２１】この検体分析用具において、多孔性シート
や容器の形成材料は、前述と同様である。In this sample analysis device, the material for forming the porous sheet and the container is the same as described above.
【００２２】[0022]
【発明の効果】以上のように、本発明の検体分析用具
は、多孔性シートと容器内壁との間に検体が浸透して、
多孔性シートの検体展開部を汚染することが防止される
ものであり、これを用いれば、正確な検査を実施するこ
とが可能である。As described above, in the sample analysis device of the present invention, the sample penetrates between the porous sheet and the inner wall of the container.
This prevents contamination of the sample developing portion of the porous sheet, and if used, it is possible to carry out an accurate test.
【図１】本発明の検体分析用具の一例を示す図であり、
（Ａ）は平面図であり、（Ｂ）は、裏面図であり、
（Ｃ）は断面図である。FIG. 1 is a view showing an example of a sample analysis tool of the present invention;
(A) is a plan view, (B) is a back view,
(C) is a sectional view.
【図２】前記例の斜視図である。FIG. 2 is a perspective view of the example.
１ 検体分析用具 ２ 多孔性シート ３ 孔 ４ スペーサー ５、７ 押え部 ６ 空気抜き部 ８ 支持部 ９ａ、９ｂ 基板 DESCRIPTION OF SYMBOLS 1 Sample analysis tool 2 Porous sheet 3 Hole 4 Spacer 5, 7 Holder 6 Air vent 8 Support 9a, 9b Substrate
───────────────────────────────────────────────────── フロントページの続き (72)発明者 田中 義行 京都府京都市南区東九条西明田町57番地 株式会社京都第一科学内 Ｆターム(参考） 2G045 AA01 AA15 BB11 CA25 CA26 CB03 CB07 DA31 FA11 FB01 FB05 FB06 FB11 FB15 GC12 HA06 HA09 HA14 HA16 ────────────────────────────────────────────────── ─── Continued on the front page (72) Inventor Yoshiyuki Tanaka 57th Higashikujo Nishiakeda-cho, Minami-ku, Kyoto-shi, Kyoto Prefecture F-term (reference) 2G045 AA01 AA15 BB11 CA25 CA26 CB03 CB07 DA31 FA11 FB01 FB05 FB06 FB11 FB15 GC12 HA06 HA09 HA14 HA16
Claims (10)
体分析用具であって、前記多孔性シートが、検体供給部
および毛細管現象により検体を展開する検体展開部を有
し、前記多孔性シートの前記検体供給部と前記検体展開
部との間の部分と、前記容器内壁との間に、毛細管現象
が発生しない大きさの空隙が形成されていることを特徴
とする検体分析用具。1. A sample analysis tool in which a porous sheet is housed inside a container, wherein the porous sheet has a sample supply section and a sample developing section for expanding a sample by capillary action, wherein the porous sheet A sample analysis tool, wherein a void having a size that does not cause a capillary phenomenon is formed between a portion between the sample supply unit and the sample development unit and the inner wall of the container.
り、この支持部により多孔性シートの検体供給部と検体
展開部との間の部分が持ち上げられることにより空隙が
形成されている請求項１記載の検体分析用具。2. A projecting support portion is formed inside the container, and a space is formed by lifting a portion of the porous sheet between the sample supply portion and the sample development portion by the support portion. Item 7. The sample analysis tool according to Item 1.
部が形成され、この押え部により多孔性シートの検体展
開部が、容器内壁に固定されている請求項２記載の検体
分析用具。3. The sample analysis tool according to claim 2, wherein a protruding pressing portion is formed inside the container on the side opposite to the support portion, and the sample developing portion of the porous sheet is fixed to the inner wall of the container by the pressing portion. .
０．１〜３ｍｍの範囲である請求項１〜３のいずれか一
項に記載の検体分析用具。4. The specimen is blood, and the maximum width of the void is:
The sample analysis tool according to any one of claims 1 to 3, which has a range of 0.1 to 3 mm.
の少なくとも一方のシートである請求項１〜４のいずれ
か一項の記載の検体分析用具。5. The sample analysis tool according to claim 1, wherein the porous sheet is at least one of a filter paper and a resin sheet.
ト厚み方向に添って連続的若しくは段階的に変化する非
対称多孔性シートである請求項１〜５のいずれか一項に
記載の検体分析用具。6. The sample analysis according to claim 1, wherein the porous sheet is an asymmetric porous sheet whose average pore diameter changes continuously or stepwise along the sheet thickness direction. Tools.
が透明である請求項１〜６のいずれか一項に記載の検体
分析用具。7. The sample analysis tool according to claim 1, wherein a part of a portion of the container corresponding to the sample developing section is transparent.
検体を展開させる検体展開部を有する多孔性シートを保
持するための容器であり、前記多孔性シートの前記検体
供給部および前記検体展開部の間の部分を持ち上げて、
毛細管現象が発生しない大きさの空隙を形成するための
突起状支持部が容器内部に形成されている容器。8. A container for holding a porous sheet having a sample supply section and a sample development section for developing a liquid sample by capillary action, wherein the container is provided between the sample supply section and the sample development section of the porous sheet. Lift the part of
A container in which a projection-like support portion for forming a void having a size that does not cause capillary action is formed inside the container.
ートを容器内壁に固定するための突起状押え部が形成さ
れている請求項８記載の容器。9. The container according to claim 8, wherein a protruding holding portion for fixing the porous sheet to the inner wall of the container is formed inside the container opposite to the support portion.
の一部が透明である請求項８または９記載の容器。10. The container according to claim 8, wherein a part of a portion corresponding to a development portion of the porous sheet is transparent.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000084352A JP3421694B2 (en) | 2000-03-24 | 2000-03-24 | Sample analysis tool and container used for it |
| EP00311639A EP1114997B1 (en) | 1999-12-28 | 2000-12-22 | Blood testing tool |
| DE60043049T DE60043049D1 (en) | 1999-12-28 | 2000-12-22 | Blood test device |
| US09/748,435 US6811753B2 (en) | 1999-12-28 | 2000-12-27 | Blood testing tool |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000084352A JP3421694B2 (en) | 2000-03-24 | 2000-03-24 | Sample analysis tool and container used for it |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2001272398A true JP2001272398A (en) | 2001-10-05 |
| JP3421694B2 JP3421694B2 (en) | 2003-06-30 |
Family
ID=18600839
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000084352A Expired - Fee Related JP3421694B2 (en) | 1999-12-28 | 2000-03-24 | Sample analysis tool and container used for it |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3421694B2 (en) |
-
2000
- 2000-03-24 JP JP2000084352A patent/JP3421694B2/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| JP3421694B2 (en) | 2003-06-30 |
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