JP2001128658A - Microorganism detector - Google Patents

Abstract

(57) [Problem] A conventional method for detecting a microorganism using an agar medium has a problem that detection of the microorganism takes time. SOLUTION: In a state where a sample repaired by a microorganism collecting material 1 is covered with a cover film 2, a dye 3 for detecting a microorganism is used, and the presence of a microorganism is detected in a short time by the presence or absence of discoloration of the dye 3. A microorganism detection device that can be used.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism detecting apparatus which can easily inspect the presence or absence of microorganisms adhering to food.

[0002]

2. Description of the Related Art In recent years, the number of cases of food poisoning has been increasing. Among them, those caused by Salmonella or Vibrio or those caused by Escherichia coli O-157 are severe and serious. Under these circumstances, the Ministry of Health and Welfare has established a hazard management system (Hassep, HACC).
It has decided to introduce P) and has approved this in the field of dairy products, some processed livestock products, and soft drinks.

[0003] Under such circumstances, there is a need for an apparatus for determining whether or not microorganisms are present. A commercially available device for detecting the presence of microorganisms is one in which a certain area is wiped off with a cotton swab, applied to an agar plate medium and cultured, and the result is determined. That is, when a microbial colony is formed on the agar plate medium, it is determined that the microorganism is present.

[0004]

The above conventional method has a problem that the presence of microorganisms cannot be directly detected. That is, the presence of microorganisms can be confirmed for the first time by the formation of microbial colonies on the agar plate medium, which takes time.

[0005]

The present invention provides a method for detecting the presence of microorganisms by detecting the presence or absence of discoloration of the microorganisms by using a microorganism detection dye in a state where the microorganisms collected by the microorganism collection material are covered with a cover film. It is a microorganism detection device that can detect in a short time.

[0006]

DETAILED DESCRIPTION OF THE INVENTION The invention described in claim 1 is a method for detecting the presence or absence of discoloration of a dye, using a dye for detecting microorganisms, with a sample collected by a microorganism collecting material covered with a cover film. The microorganism detection device can detect the presence of microorganisms in a short time.

According to a second aspect of the present invention, an adhesive substance is used as a microorganism-collecting material.
The microorganism detection device can easily collect microorganisms and detect the presence of microorganisms in a short time.

According to a third aspect of the present invention, an electrostatically processed non-woven fabric is used as a microorganism collecting material.
The microorganism detection device can easily collect microorganisms and detect the presence of microorganisms in a short time.

According to a fourth aspect of the present invention, the cover film has a release material on one side, and the surface having the release material is provided with a microorganism-collecting material and a dye for detecting microorganisms. This is a microorganism detection device that can prevent contamination and perform accurate measurement.

[0010] The invention described in claim 5 is a microorganism detecting apparatus which can prevent an erroneous reaction at the time of storage and can perform accurate measurement by using a dye for detecting microorganisms which is individually mounted.

The invention described in claim 6 is a microorganism detecting apparatus which dyes peptidoglycan as a dye for detecting microorganisms, has a low reaction threshold value, and enables accurate measurement.

[0012] The invention described in claim 7 is a microorganism detection device excellent in reactivity and safety, wherein the dye for detecting microorganisms is derived from silkworm or horseshoe crab.

According to an eighth aspect of the present invention, there is provided the microorganism detecting device according to any one of the first to seventh aspects, and a dye for detecting the microorganism is ruptured by pressing a surface of the microorganism detecting device when detecting the microorganism. Then, as a configuration provided with equipment for spreading the dye on the surface of the microorganism collecting material, the microorganism detecting device can detect the presence or absence of microorganisms very easily.

According to a ninth aspect of the present invention, the equipment is a roller, and the microorganism detection dye can be uniformly distributed on the entire surface of the microorganism collection material by using the roller, so that the detection of the microorganism can be easily and surely performed. And

[0015]

(Embodiment 1) Hereinafter, a first embodiment of the present invention will be described. FIG. 1 is an explanatory diagram showing the configuration of the present embodiment. The microorganism detection device of the present embodiment includes a substrate 4 and a substrate 4
The microorganism collecting material 1 and the dye 3 for detecting microorganisms provided above
And a cover film 2 that covers the microorganism-collecting material 1 and the dye 3 for detecting microorganisms.

In this embodiment, as the microorganism-collecting material 1, an adhesive material such as silicon rubber or epoxy resin, or a nonwoven fabric made of electrostatically processed polyethylene is used.

As the dye 3 for detecting microorganisms, a protein having the property of dissolving bacterial cell membranes to decompose into a peptidoglycan structure and reacting with the decomposed peptidoglycan is used. The protein bound to the peptidoglycan takes the reaction pathway shown in Chemical formula 1 and produces melamine as a final product. This melamine has a black color. Although the composition of the protein has not been elucidated, it is a protein derived from a natural product and is a bodily fluid exuding by damaging the epidermis of a silkworm or horseshoe crab. Although body fluids can be extracted from various organisms including sea cucumber and starfish, only the body fluids of silkworm and horseshoe crab react with the cell membrane of microorganisms. This 2
Dyes derived from various natural products have no effect on the human body and are highly safe.

[0018]

Embedded image

The operation of this embodiment will be described below.
As shown in FIG. 1, the surface of a microorganism collecting material 1 and a surface of a microorganism detection dye 3 are covered with a cover film 2 in the microorganism detection device of the present embodiment. At this time, the surface of the cover film 2 that is in contact with the microorganism-collecting material 1 and the microorganism-detecting dye 3 is coated with a release material.

The user peels off the cover film 2 once and uses the microorganism-collecting material 1 to lightly wipe the surface of the detection target. As the detection target, for example, a kitchen knife or a cutting board placed in food or a kitchen, a kitchen in a hospital, or the like can be considered.

At this time, in this embodiment, the microorganism collecting material 1 is used.
The sample can be collected very easily because a sticky material such as silicone rubber or epoxy resin or a non-woven fabric made of electrostatically processed polyethylene is used. The reason for using an electrostatically processed polyethylene nonwoven fabric is that microorganisms are usually negatively charged. When the collection of the sample is completed in this way, the cover film 2 is again attached to the surface of the microorganism collecting material 1 and the surface of the microorganism detecting dye 3 to cover the same. This operation can be easily performed because the cover film 2 has a release material on one side. In addition, since the surfaces of the microorganism-collecting material 1 and the microorganism-detecting dye 3 are covered with the cover film 2 before use, foreign matter does not adhere to the surface of the microorganism-collecting material 1 and, therefore, accurate. It can be measured.

When pressure is applied from above the cover film 2 while the surface is covered with the cover film 2,
The microorganism detecting dye 3 oozes out and the microorganism collecting material 1
Reacts with the sample adhering to the surface.
That is, if microorganisms are present in the sample,
It follows the route shown in Chemical formula 1 to produce melamine and exhibit black color. The time required for this reaction is several minutes, and the presence or absence of microorganisms can be easily determined on the spot.

When the microorganism detection dye 3 is used individually, it is possible to prevent an erroneous reaction during storage and to realize a microorganism detection device capable of performing accurate measurement.

Next, a description will be given of an experiment in which the inventors confirmed the detection sensitivity of the microorganism detection apparatus of this embodiment. As a general means for measuring microorganisms, a method of culturing and measuring microorganisms using an agar plate medium has been conventionally used. The measurement limit of this method is 30 / ml. On the other hand, when the apparatus of the present embodiment is used, it is possible to reliably measure up to 1 sample / ml. That is, using the microorganism detection dye 3 used in this example, the bacterial
When measured with an aqueous solution diluted by a factor of 1, 1 / ml
It is possible to obtain a reaction exhibiting a black color.
Furthermore, as a microorganism-collecting material 1, a bacterial nonwoven fabric of Escherichia coli was used at a rate of 100 cells / ml,
After dropping 10 / ml and 1 / ml respectively, the bacterial solution was dried at 5 ° C. and immersed in the microorganism detection dye 3. As a result, black spots can be confirmed even when the bacterial solution of Escherichia coli was prepared at 1 / ml. In other words, the measurement limit of microorganisms in the apparatus of this embodiment is 1 / ml. That is, the present invention realizes a microorganism detection device having a low reaction threshold value and capable of performing accurate measurement.

(Embodiment 2) Next, a second embodiment of the present invention will be described. FIG. 2 is a sectional view showing the configuration of the present embodiment. In the present embodiment, a roller 5 is used to press down the surface of the cover film 2 of the microorganism detection device to rupture the dye for detecting microorganisms and to spread the dye on the surface of the microorganism collection material when detecting microorganisms. . Roller 5
Instead of using a fixture such as a plate-shaped holding plate, there is no problem. As described above, the microorganism can be easily detected. That is, the dye 3 for detecting microorganisms can be evenly distributed throughout the microorganism-collecting material 1, and the detection of microorganisms can be easily and reliably performed.

[0026]

According to the first aspect of the present invention, there is provided a substrate, a microorganism-collecting material and a dye for detecting microorganisms provided on the substrate,
As a configuration including a cover film that covers the microorganism collecting material and the dye for detecting microorganisms, the present invention realizes a microorganism detecting device that can detect the presence of microorganisms in a short time by the presence or absence of discoloration of the dye.

According to a second aspect of the present invention, there is provided a construction wherein a substance having tackiness is used as a microorganism collecting material.
An object of the present invention is to realize a microorganism detecting device that can easily collect microorganisms and detect the presence of microorganisms in a short time.

According to a third aspect of the present invention, there is provided a method of using an electrostatically processed nonwoven fabric as a microorganism collecting material.
An object of the present invention is to realize a microorganism detection device capable of detecting the presence of microorganisms in a short time.

According to a fourth aspect of the present invention, the cover film has a release material on one side, and the surface having the release material is disposed on a surface on which a microorganism collecting material and a dye for detecting microorganisms are disposed. As a result, the present invention realizes a microorganism detection device capable of preventing contamination during storage and performing accurate measurement.

The invention described in claim 5 realizes a microorganism detecting apparatus which can prevent an erroneous reaction at the time of preservation and can perform accurate measurement by using an individual dye for detecting microorganisms. It is.

The invention described in claim 6 realizes a microorganism detecting apparatus which has a low reaction threshold value and can perform accurate measurement, as a dye for detecting microorganisms stains peptidoglycan as a target.

The invention described in claim 7 realizes a microorganism detection device excellent in reactivity and safety, wherein the dye for detecting microorganisms is derived from silkworm or horseshoe crab.

According to an eighth aspect of the present invention, there is provided a microorganism detecting apparatus according to any one of the first to seventh aspects, and a dye for detecting a microorganism is ruptured by pressing a surface of the microorganism detecting apparatus when detecting a microorganism. The present invention realizes a microorganism detecting device that can very easily detect the presence or absence of microorganisms as a structure having a device for spreading a dye on the surface of the microorganism collecting material.

According to the ninth aspect of the present invention, there is provided a microorganism detecting apparatus capable of detecting the presence or absence of microorganisms very easily by using the equipment as a roller.

[Brief description of the drawings]

FIG. 1 is a cross-sectional view illustrating a configuration of a microorganism detection device according to a first embodiment of the present invention.

FIG. 2 is a cross-sectional view showing a configuration of a microorganism detection device according to a second embodiment.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 microorganism collecting material 2 cover film 3 microorganism detecting dye 4 substrate 5 roller

 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Akiko Inuzuka 1006 Kazuma Kadoma, Osaka Prefecture Matsushita Electric Industrial Co., Ltd.F-term (reference) QA01 QA18 QQ06 QQ07 QQ16 QR48 QR66 QS02 QS15 QS36 QS39 QX01

Claims (9)

[Claims] 1. A microorganism detecting apparatus comprising: a base material; a microorganism collecting material and a dye for detecting microorganisms provided on the base material; and a cover film covering the microorganism collecting material and the dye for detecting microorganisms. 2. The microorganism detecting device according to claim 1, wherein a substance having tackiness is used as the microorganism collecting material. 3. The microorganism detecting device according to claim 1, wherein a nonwoven fabric subjected to electrostatic processing is used as the microorganism collecting material. 4. The cover film according to claim 1, wherein the cover film has a release material on one side, and the surface having the release material is disposed on a surface on which a microorganism collecting material and a dye for detecting microorganisms are disposed. 2. The microorganism detection device according to item 1. 5. The microorganism detecting device according to claim 1, wherein the dye for detecting the microorganism is individually packaged. 6. The microorganism detecting device according to claim 1, wherein the dye for detecting a microorganism stains peptidoglycan. 7. The dye according to claim 1, wherein the dye for detecting microorganisms is derived from silkworm or horseshoe crab.
The microorganism detection device according to the paragraph. 8. A microorganism-collecting material, comprising: a microorganism-detecting device according to claim 1; and a microorganism-collecting material, wherein the microorganism-detecting material is ruptured by pressing a surface of the microorganism-detecting device when detecting microorganisms. A microorganism detection device having equipment for spreading a dye on the surface of a microorganism. 9. The microorganism detecting apparatus according to claim 8, wherein the equipment is a roller.