JP2001072572A - Skin preparation for external use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a skin preparation which is used for external use, can effectively utilize fish skins which are industrial wastes, can sufficiently exhibit the moisture-retaining and skin-protecting effects of elastin, and is useful for preventing the aging of skins. SOLUTION: This skin preparation for external use contains a hydrolyzed elastin solution originated from fish skins, and an ingredient selected from a cell-activating ingredient and an anti-inflammatory ingredient. The cell- activating ingredient includes 2-hydroxycarboxylic acids, specific vitamin-like active substances, nucleic acid-related substances, serum-removed protein extracts, spleen extracts, placenta extracts, cockscom extracts, royal jelly, Ganoderma lucidum extracts, yeast extracts, lactobacillus extracts, bifidobacterium extracts, and bittern ingredients. The anti-inflammatory ingredient includes glycyrrhizic acid, glycyrrhetic acid, isopropylaminocaproic acid, azulene, lysozyme, salicylic acid, 7-oryzanol, allantoin, their derivatives, and their salts.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an external use for skin which has a synergistic enhancement of moisturizing action and dermal fibroblast activating action and is effective for preventing or improving skin aging symptoms such as wrinkles and reduced skin elasticity. Agent. More specifically, the present invention relates to a skin external preparation comprising a fish skin-derived hydrolyzed elastin solution and one or more selected from a cell activating component and an anti-inflammatory component.

[0002]

2. Description of the Related Art Elastin is a main component of elastic fibers, is an insoluble protein, and is present in highly elastic organs such as ligaments and skin. This elastin has been expected to be incorporated into cosmetics as a skin protection and moisturizing agent. However, since elastin is insoluble, it has been difficult to incorporate it in a cosmetic formulation as it is. Therefore, attempts have been made to solubilize and mix elastin. For example, cosmetics containing soluble hydrolyzed elastin (JP-A-53-72832), water-soluble natural elastin and / or
Or a hair and skin cosmetic containing an elastin hydrolyzate solubilized by an enzyme (JP-A-54-10523)
8), elastin for cosmetics solubilized by elastase (JP-A-58-192812), skin cosmetics using soluble elastin and / or solubilized elastin in combination with a humectant and / or a physiologically active substance (JP-A-59-1982). 13930
8), an oil-in-water emulsion for skin containing an elastin hydrolyzate as an emulsifier (JP-A-59-23100)
7), a composition for skin and hair containing elastin derived from bovine ligament having a molecular weight of 15,000 to 300,000 (JP-A-60-258107), and an elastin hydrolyzate having a molecular weight of 200,000 or more was compounded as an emulsifier. A method of stabilizing an elastin hydrolyzate aqueous solution with an emulsified composition (JP-A-63-302934), 1,3-butylene glycol and propylene glycol (JP-A-2-16721)
Techniques such as 1) are disclosed. However, most of these elastin hydrolysates are based on domestic animals such as cattle, pigs, and chickens.

It is already known that fish skin is rich in elastin. However, fish skin generated from the fishery processing industry is regarded as industrial waste and is disposed of in large quantities throughout the year. Recent research on the effective use of industrial wastes has disclosed a technique for hydrolyzing elastin from fish skin and isolating it together with collagen (Japanese Patent Laid-Open No. 5-202097).

[0004]

In the present invention, fish skin, which is an industrial waste, is effectively used, and the effect of elastin on the moisturizing and skin protection is sufficiently exhibited, which is useful for preventing skin aging. It is an object of the present invention to provide a skin external preparation.

[0005]

Means for Solving the Problems The present inventor conducted a study in view of the above problems, and as a result, selected from a fish skin-derived hydrolyzed elastin solution, a cell activating component and an anti-inflammatory component.
By blending the seed or two or more components with the external preparation for skin, the moisturizing and skin-protecting effects of elastin can be sufficiently exerted with a small amount of blending, and furthermore, the present invention has been found to be excellent in preventing skin aging. It was completed.

[0006]

Embodiments of the present invention will be described.

The hydrolyzed elastin solution derived from fish skin used in the present invention is a solution obtained by hydrolyzing fish skin using an acid or an alkali, or a proteolytic enzyme such as elastase, pepsin, and trypsin. At that time, the pH of the fish skin-derived hydrolyzed elastin solution is determined in consideration of the effect on other components and the formulation to be mixed in the external preparation for skin.
It is preferable to adjust the value in the range of 3.5 to 4.5. The type of fish from which the hydrolyzed elastin solution derived from fish skin is obtained is not particularly limited, and examples thereof include cod, mackerel, salmon, herring, thailand, tuna, and sardine.

[0008] The hydrolyzed elastin solution derived from fish skin used in the present invention is, for example, crushed cod skin, mixed with purified water, heated at 95 ° C for 1 hour, and added elastase at 40 ° C. Perform partial hydrolysis for 30 hours, then 95
It can be prepared by heating at 1 ° C. for 1 hour to stop the enzymatic reaction, adjusting the pH to 4.0, and performing sterile filtration with a membrane filter.

[0009] The amount of the hydrolyzed elastin solution derived from fish skin used in the present invention in the external preparation for skin is considered to be 0.0001 to 5.0% by weight in consideration of its effect and stability of the preparation. Is preferred.

In the skin external preparation of the present invention, one or more selected from a cell activating component and an anti-inflammatory component are blended.

The cell activating component may be of any type and origin, as long as it is used in ordinary skin external preparations. For example, the cell activating component is 2-hydroxycarboxylic acid having 2 to 22 carbon atoms and salts and derivatives thereof, vitamin C, E,
Vitamin-like active substance except P-related compound, nucleic acid-related substance, serum deproteinized extract, spleen extract, placenta extract, cockscomb extract, royal jelly, reishi extract, yeast extract, lactic acid extract, bifidobacterium An extract, a bittern component, etc. are illustrated.

The α-hydroxycarboxylic acid having 2 to 22 carbon atoms and its salts and derivatives thereof having a cell activating effect used in the present invention is not particularly limited. ~ 6 short chain α-
Hydroxycarboxylic acids such as α-hydroxyacetic acid,
Improved dermal absorption of lactic acid, malic acid, tartaric acid, pyruvic acid, citric acid and salts thereof, and α-hydroxy acid.
-Alkyl esters of hydroxy acids, cholesterol esters, glycosides, phosphatidyl esters and the like are preferably used.

Further, as vitamin-like active substances excluding vitamin C, E and P-related compounds, vitamin A such as vitamin A oil and retinol acetate; vitamin B2 such as riboflavin and riboflavin butyrate; and vitamin B6 such as pyridoxine hydrochloride. And nicotinic acids such as L-nicotinic acid, nicotinamide and benzyl nicotinate, and vitamin Hs such as biotin.

The nucleic acid-related substance used in the present invention is not particularly limited, but includes deoxyribonucleic acid and salts thereof, adenylic acid derivatives such as adenosine triphosphate and adenosine diphosphate and salts thereof, ribonucleic acid and salts thereof, and guanine. , Xanthine and their derivatives and their salts.

The type and base of the anti-inflammatory component are not limited as long as it is used in ordinary skin external preparations. Examples thereof include glycyrrhizic acid, glycyrrhetinic acid, isopropylaminocaproic acid, azulene, lysozyme, salicylic acid, γ-oryzanol, allantoin, derivatives thereof, and salts thereof.

The amount of the above-mentioned cell activating component and anti-inflammatory component to be added to the external preparation for skin may be 0.0001 to 5 in consideration of the effect on the stability of the preparation and the bioavailability.
A suitable amount is about weight%.

In the present invention, a skin external preparation containing a hydrolyzed elastin solution derived from fish skin and one or more selected from a cell activating component and an anti-inflammatory component can be provided. Can take the form of lotions, emulsions, creams, ointments and the like. Furthermore, lotions such as flexible lotion, astringent lotion, lotion for washing, emollient cream, moisture cream,
Creams such as massage creams, cleansing creams, and makeup creams, emulsions such as emollient emulsions, moisture emulsions, nourishing emulsions, and cleansing emulsions, packs such as jelly packs, peel-off packs, rinse-off packs, powder packs, and serums. And cosmetic preparations of various preparation forms such as face wash.

In the present invention, other active ingredients such as a whitening ingredient, a humectant, an antioxidant, an ultraviolet absorber and the like can be used in combination, and a sunscreen cosmetic, an antiaging cosmetic, a whitening agent, etc. Can be provided as cosmeceuticals or quasi-drugs.

[0019]

EXAMPLES First, a production example of a hydrolyzed elastin solution derived from fish skin used in Examples of the present invention will be described.

[Manufacturing Example 1] Cod skin-derived elastin solution Frozen cod skin is ground and mixed with 3 times by weight of purified water. After heating this mixture to 95 ° C., it is allowed to stand and cool. Elastase is added and reacted at 40 ° C. for 30 hours to partially hydrolyze. The enzyme reaction is then stopped by heating at 95 ° C. for 1 hour and filtered to remove unhydrolysates and residual fat. Furthermore, sterilization filtration was performed using a membrane filter, and an elastin solution derived from cod skin was obtained. The pH of this elastin solution derived from cod skin is 4.0.
Met.

The characteristics of the present invention will be described in detail with reference to Examples using the cod skin-derived elastin solution shown in Production Example 1.

A skin cream was prepared by using the cell activating component, the anti-inflammatory component, and the cod skin-derived elastin solution shown in Production Example 1 as shown in Table 1, according to the following formulation.

[Examples 1 to 4 and Comparative Examples 1 to 6] Skin cream (1) Beeswax 6.0 (% by weight) (2) Cetanol 5.0 (3) Reduced lanolin 8.0 ( 4) Squalane 37.5 (5) Fatty acid glycerin 4.0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene (20EO) sorbitan monolaurate 2.0 (8) Propylene glycol 0 (9) Methyl parahydroxybenzoate 0.1 (10) Purified water Amount based on the total amount of 100 (11) Components shown in Table 1 As described in Table 1 (12) Fragrance 0.2 Production method: (1)- The oil phase component of (7) is mixed and dissolved to make it uniform, and heated to 75 ° C. On the other hand, the aqueous phase components (8) to (10) are mixed and dissolved and heated to 75 ° C. Next, after the oil phase component is added to the water phase component and pre-emulsified, the mixture is uniformly emulsified by a homomixer. After that, it is cooled and at 50 ° C (11),
Add (12) and mix.

[0024]

[Table 1]

The above Examples 1 to 4 and Comparative Examples 1 to
6 was subjected to a three-month actual use test. As a panelist, 40s to 60s worried about skin dryness
Using men and women in their twenties, women in their forties to sixties who have skin symptoms such as remarkable wrinkles or reduced elasticity, and women in their twenties to fifties who exhibit remarkable skin roughness, There were 20 people in each group. In the use test, each group was allowed to use each of the examples and comparative examples with blinds,
The state of the skin was observed before the start of the use test and after the end of the use test. The drying symptoms, wrinkles and skin elasticity are shown in Table 2 in terms of the number of panels who answered that the skin after 3 months was moistened and the wrinkles and skin elasticity were improved compared to before use. Regarding the roughness of the skin, the skin condition was scored according to the criteria shown in Table 3, and the results before and after the end of the use test were compared in Table 4 based on the average value of 20 persons.

[0026]

[Table 2]

[0027]

[Table 3]

[0028]

[Table 4]

As shown in Table 2, in the group using the examples of the present invention, 90% or more of panelists showed improvement in dryness, wrinkles and skin elasticity. On the other hand, in the use groups of Comparative Examples 1 to 5, the drying symptoms, wrinkles, and the tendency of improvement in elasticity were observed as compared with Comparative Example 6 in which various components were not blended.
No more than nine panelists showed a clear improvement trend. Further, as shown in Table 4, in the group using the example of the present invention, improvement tendency of the skin roughness was observed in all the panelists, and in most of the panelists, the skin sulcus and skin ridges were clearly observed. On the other hand, in Comparative Examples 1 to 5 in which various components were independently blended, the skin condition was improved compared to Comparative Example 6 in which various components were not blended. The shape remained ambiguous.

In Examples 1 to 4 of the present invention, no change in the state of the preparation such as precipitation, separation, agglomeration, discoloration, and discoloration of the components was observed during the use test period. In addition, in each group used in Examples, there was no paneler showing a skin irritating reaction or a skin sensitizing reaction.

Next, the formulation of another embodiment of the present invention will be described.

Example 5 Hair restorer (1) Purified water 50.0 (% by weight) (2) Ethanol 49.2 (3) Elastin solution derived from cod skin (Production Example 1) 0.3 (4) Allantoin 5 Production method: Mix and homogenize components (1) to (4).

Example 6 Whitening Liquid (1) Squalane 5.0 (% by weight) (2) White Vaseline 2.0 (3) Beeswax 0.5 (4) Sorbitan Sesquioleate 0.8 (5) Poly Oxyethylene oleyl ether (20EO) 1.2 (6) Methyl paraoxybenzoate 0.1 (7) Propylene glycol 5.0 (8) Purified water 58.6 (9) Carboxyvinyl polymer (1% by weight aqueous solution) 0 (10) Potassium hydroxide 0.1 (11) Elastin solution derived from cod skin (Production Example 1) 0.5 (12) Ethanol 5.0 (13) Royal jelly 1.0 (14) Fragrance 0.2 Production method: The oil phase components (1) to (5) are mixed and heated to 75 ° C. to dissolve and homogenize. On the other hand, the aqueous phase components (6) to (8) are mixed and dissolved, heated to 75 ° C., and the oil phase component is added and pre-emulsified. After adding (9), the mixture is emulsified uniformly with a homomixer, and (10) is added to adjust the pH. 40 after cooling
Add (11) to (14) at ℃ and mix.

Example 7 Skin Lotion (1) Ethanol 10.0 (% by weight) (2) Hydroxyethylcellulose 1.0 (3) Cod skin-derived elastin solution (Production Example 1) 0.5 (4) Paraoxyl Methyl benzoate 0.1 (5) Glycerin 7.0 (6) Sodium guaiazulene sulfonate 0.5 (7) Purified water 80.9 Production method: Mix (1) to (7) to make uniform.

Example 8 Skin Emulsion (1) Stearic acid 0.2 (% by weight) (2) Cetanol 1.5 (3) Vaseline 3.0 (4) Liquid paraffin 7.0 (5) Polyoxy Ethylene (10EO) monooleate 1.5 (6) Lactic acid bacteria extract 0.5 (7) Glycerin 5.0 (8) Methyl paraoxybenzoate 0.1 (9) Triethanolamine 1.0 (10) Purification Water 79.2 (11) Cod skin-derived elastin solution (Production Example 1) 1.0 Production method: The oil phase components (1) to (6) are mixed, heated and uniformly dissolved, and kept at 70 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and heated to be uniform, and the temperature is set to 70 ° C. The oil phase component is gradually added to the aqueous phase component while stirring to emulsify, and after cooling, the component (11) is added and mixed at 40 ° C.

Example 9 Skin Gel (1) Purified Water 89.3 (% by weight) (2) Carboxyvinyl Polymer 0.5 (3) Dipropylene Glycol 8.0 (4) Methyl Paraoxybenzoate 0 0.1 (5) Potassium hydroxide 0.1 (6) Cod skin-derived elastin solution (Production Example 1) 1.0 (7) Placental extract 1.0 Production method: (2) was uniformly dissolved in (1) Then, (4) was dissolved and added to (3), and then (5) was added to increase the viscosity, (6),
Add the component of (7).

Example 10 Skin Cream (1) Beeswax 6.0 (% by weight) (2) Cetanol 5.0 (3) Reduced Lanolin 8.0 (4) Squalane 29.5 (5) Lipophilic type Glyceryl monostearate 4.0 (6) Polyoxyethylene (20EO) sorbitan monolaurate 5.0 (7) Propylene glycol 5.0 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water 35.4 (10) Elastin solution derived from cod skin (Production Example 1) 1.0 (11) Sodium lactate 1.0 Production method: The oil phase components (1) to (6) are mixed and dissolved and heated to 75 ° C. On the other hand, the aqueous phase components (7) to (9) are mixed and dissolved and heated to 75 ° C. Next, the oil phase component is added to the water phase component and preliminarily emulsified, and then uniformly emulsified by a homomixer. After cooling, the components (10) and (11) are added and mixed at 40 ° C.

[Example 11] Oil-in-water emulsion ointment (1) White petrolatum 25.0 (wt%) (2) Stearyl alcohol 25.0 (3) Glycerin 10.0 (4) Sodium lauryl sulfate 1.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 36.9 (7) Cod skin-derived elastin solution (Production Example 1) 1.0 (8) Lactic acid bacterium fermented liquid 1.0 Production method: (1) to ( The oil phase component of 4) is mixed and dissolved to make it uniform, and heated to 75 ° C. On the other hand, dissolve (5) in (6)
Heat to 5 ° C, add the oil phase component to this and emulsify,
After cooling, the components (7) and (8) are added and mixed at 40 ° C.

Example 12 Lotion (1) Ethanol 10.0 (% by weight) (2) 1,3-butylene glycol 10.0 (3) Cod skin-derived elastin solution (Production Example 1) 0.5 ( 4) Dipotassium glycyrrhizinate 0.5 (5) Fragrance 0.1 (6) Purified water 78.9 Production method: (1) to (5) are sequentially added to (6) and uniformly mixed and dissolved.

Example 13 Water-in-oil emulsifying emollient cream (1) Liquid paraffin 36.0 (% by weight) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglyceryl oleic acid Ester 5.0 (5) γ-oryzanol 1.0 (6) Propylene glycol 3.0 (7) Methyl paraoxybenzoate 0.1 (8) Purified water 47.3 (9) Cod skin-derived elastin solution (Production example) 1) 0.5 (10) Fragrance 0.1 Production method: The oil phase components (1) to (5) are mixed and dissolved to make uniform, and heated to 75 ° C. On the other hand, the aqueous phase components (6) to (9) are mixed and dissolved to make uniform, heated to 75 ° C., added to the oil phase component, and emulsified by a homomixer. 40 ° C after cooling
Add and mix component (10) with.

Example 14 Makeup Base Cream (1) Stearic acid 12.0 (% by weight) (2) Cetanol 2.0 (3) Glyceryl tri-2-ethylhexanoate 2.5 (4) Self Emulsified glyceryl monostearate 2.0 (5) Propylene glycol 10.0 (6) Potassium hydroxide 0.3 (7) Purified water 67.6 (8) Titanium oxide 1.0 (9) Bengala 0.1 (10) Yellow iron oxide 0.4 (11) Fragrance 0.1 (12) Cod skin-derived elastin solution (Production Example 1) 1.0 (13) Cockscomb extract 1.0 Production method: (1) to (4) Are mixed and heated to 75 ° C. to make uniform. On the other hand, the components (5) to (7) were mixed and 75
The mixture was heated and melted at ℃ to make it uniform, and the pigments (8) to (10) were added thereto and uniformly dispersed with a homomixer to obtain an aqueous phase component. The oil phase component is added to the aqueous phase component, emulsified by a homomixer, cooled, and the components (11) to (13) are added and mixed at 40 ° C.

Example 15 Emulsion Foundation (1) Stearic acid 2.0 (% by weight) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5) Decaglyceryl monoisopalmitate 9.0 (6) 1,3-butylene glycol 8.0 (7) Potassium hydroxide 0.1 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water 50.8 ( 10) Titanium oxide 9.0 (11) Talc 7.4 (12) Bengala 0.5 (13) Yellow iron oxide 1.1 (14) Black iron oxide 0.1 (15) Fragrance 0.1 (16) Cod Skin-derived elastin solution (Production Example 1) 0.5 (17) Dried seawater powder (Nigari) 0.3 Production method: Mix oil phase components (1) to (5) and heat to 75 ° C to make uniform . On the other hand, the aqueous phase components (6) to (9) are mixed,
Heat and dissolve to 75 ° C to make uniform, add the pigments of (10) to (14) to the mixture, emulsify uniformly with a homomixer, cool, and cool at 40 ° C to (15) to (17). Add and mix the ingredients.

Example 16 Hand cream (1) Cetanol 4.0 (% by weight) (2) Vaseline 2.0 (3) Liquid paraffin 10.0 (4) Glyceryl monostearate 1.5 (5) Polyoxyethylene (60EO) glyceryl isostearate 2.5 (6) Tocopherol acetate 0.5 (7) Glycerin 20.0 (8) Methyl paraoxybenzoate 0.1 (9) Purified water 57.4 (10) Cod Skin-derived elastin solution (Production Example 1) 1.0 (11) Serum deproteinized extract 1.0 Production method: Mix and dissolve the oil phase components (1) to (6) and heat to 75 ° C. On the other hand, the aqueous phase components (7) to (9) are mixed and dissolved and heated to 75 ° C. Next, the oil phase component is added to the water phase component and pre-emulsified, then uniformly emulsified and cooled with a homomixer, and the components (10) and (11) are added and mixed at 40 ° C.

[0044]

According to the present invention, a skin external preparation containing a hydrolyzed elastin solution derived from fish skin and a cell activating component and an anti-inflammatory component has a moisturizing effect and a dermal fibroblast activating effect that are synergistically enhanced. It was shown to be effective in preventing or improving skin aging symptoms such as a decrease in skin elasticity.

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Claims (3)

[Claims] 1. A hydrolyzed elastin solution derived from fish skin,
An external preparation for skin containing one or more components selected from cell activating components and anti-inflammatory components. 2. The cell activating component is a 2-hydroxycarboxylic acid having 2 to 22 carbon atoms and salts and derivatives thereof, vitamin-like active substances excluding vitamin C, vitamin E and vitamin P-related compounds, nucleic acid-related substances, serum deprivation. One or more selected from protein extract, spleen extract, placenta extract, cockscomb extract, royal jelly, ganoderma extract, yeast extract, lactic acid bacteria extract, bifidobacterium extract, bittern component The external preparation for skin according to claim 1, which is present. 3. The anti-inflammatory component is one or more selected from glycyrrhizic acid, glycyrrhetinic acid, isopropylaminocaproic acid, azulene, lysozyme, salicylic acid, γ-oryzanol, allantoin and derivatives thereof, and salts thereof. The external preparation for skin according to claim 1, which is present.