JP2000325040A - Learning/memory capacity-improving food - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject food containing docosahexaenoic acid, ginkgo leaves extract, lecithin and tocopherol. SOLUTION: This learning/memory capacity-improving food contains docosahexaenoic acid, ginkgo leaves extract, lecithin and tocopherol. The food is more effective than those containing DHA and ginkgo leaves extract and those containing lecithin and tocopherol, therefore being excellent in learning/ memory capacity-improving effect.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a food having improved learning and memory ability, comprising docosahexaenoic acid (hereinafter referred to as "DHA"), ginkgo biloba extract, lecithin and tocopherol.

[0002]

2. Description of the Related Art In recent years, attention has been paid to the improvement of the learning and memory ability of DHA and the function of preventing and recovering senile dementia. However, at present, there are few drugs that can effectively treat cerebral dysfunction such as senile dementia, and establishment of a treatment method is desired. Ginkgo biloba extract has the effect of improving blood flow by expanding blood vessels to improve cerebral blood circulation, and is effective in reducing memory and improving senile dementia. In Europe, a medicine containing ginkgo biloba extract as a main component has been approved as a brain and peripheral circulation improving agent. Conventionally, these DHA and ginkgo biloba leaf extract have been used alone. Furthermore, an attempt has been made to obtain a synergistic effect by combining these two (Japanese Patent Application Laid-Open No. Hei 6-340539).

[0003]

The synergistic effect of the combination of DHA and Ginkgo biloba extract is recognized as compared with the case of using DHA and Ginkgo biloba extract alone, but the effect alone is not sufficient. In view of such circumstances, the present inventors have studied to obtain a food with improved learning and memory ability, and as a result, by combining DHA, ginkgo biloba extract, lecithin and tocopherol, furthermore, these components were identified. It has been found that a particularly remarkable effect can be exerted by combining them at the ratio described above, and based on this finding, the present invention has been completed.

[0004]

That is, the present invention provides a DH
A, G / Ginkgo leaf extract, lecithin and tocopherol-containing food with improved learning and memory ability.

[0005] DHA is called docosahexaenoic acid, and is contained abundantly in fish, crustaceans, marine animals and the like. Above all, D
As the HA resources, DHA-containing fish oil obtained by deacidifying, decolorizing, concentrating and deodorizing fish oils such as sardines, mackerel, skipjack, and tuna can be used. Further, by further refining the DHA-containing fish oil, a DHA-rich fish oil can be obtained, or DHA itself (pure substance) may be used.

Ginkgo biloba, a ginkgo biloba extract used in the present invention, is a plant belonging to the family Ginkgo which belongs to the family Ginkgo and is used in health foods, folk medicine and the like. That is, the ginkgo biloba extract used in the present invention means the above ginkgo biloba leaves or a solvent extract thereof. As the solvent used for the extraction, any solvent can be used as long as it is a solvent from which the component exhibiting the learning / memory ability improving effect of the present invention can be extracted. For example, water, lower alcohols (methanol, ethanol, propanol, butanol, etc.), ketone (Acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.), chloroform, dichloromethane And the like. Preferably, polar solvents such as water, the above lower alcohols and ketones are used. These solvents may be used alone or in combination of two or more. The extraction may be carried out at normal temperature or by heating, and the obtained extract can be further purified, concentrated, filtered, etc., if necessary. The ginkgo biloba extract may be in the form of an extracted solution, or may be subjected to treatments such as freeze-drying, spray-drying, and concentration-drying to obtain a dried product, and the form of use is not particularly limited. The food for improving learning and memory ability of the present invention is characterized in that it contains a component exhibiting the effect of improving learning and memory ability, and contains shredded, crushed, and crushed ginkgo leaves. Although it may be present, it is more preferable to contain an extract extracted with a solvent. Furthermore, the solvent extract may be purified by chromatography or the like, and it is particularly preferable to contain the flavone glycoside at a high concentration.

As lecithin, phosphatidylcholine itself, soybean lecithin containing phosphatidylcholine, egg yolk lecithin, rapeseed lecithin and purified lecithin obtained by purifying these can be used. Purified lecithin includes powdered (high purity) lecithin, fractionated lecithin and the like.

[0008] Tocopherol is a natural vitamin E, and it is possible to use tocopherol or a highly concentrated tocopherol from vegetable oil containing tocopherol. Tocopherol includes any homologues of α, β, γ and σ.

As the above-mentioned DHA, ginkgo biloba extract, lecithin and tocopherol, any commercially available one may be used, and if necessary, a commercially available product may be repurified and used.

The form of the food for improving learning and memory ability according to the present invention is not particularly limited. Examples thereof include tablets, granules, capsules, powders, gums, chocolate, candy, noodles, bread, cakes, biscuits, cooking oil, Dressing, canned food, retort food, livestock meat food, seafood paste, margarine, butter,
Normal food forms such as mayonnaise and beverages can be employed. Among them, capsules, tablets, granules, cooking oil,
Dressing and the like are preferred.

In the case of producing the food, if necessary, gelatin, methylcellulose, shellac and other film-forming agents, glycerin, fatty acid esters and sucrose fatty acid esters and other active agents, lactose, starch and dextrin, etc. Ingredients, fats and oils, wax components such as beeswax and rice sugar wax, and nutrient-enhancing components such as vitamins, minerals and amino acids can be blended.

[0012] DHA, ginkgo biloba extract according to the present invention,
Lecithin and tocopherol can be blended in any ratio, but the amount of DHA / ginkgo biloba extract = 1-6, the amount of tocopherol / the amount of phosphatidylcholine in lecithin =
1 to 50, (tocopherol + phosphatidylcholine in lecithin) / (DHA + ginkgo biloba extract) =
It is more preferable to mix them in a composition of 0.022 to 0.24.

[0013]

EXAMPLES Next, the present invention will be described in detail with reference to Examples, but the present invention is not limited thereto. The parts of the amounts shown in the examples are parts by weight.

Example 1 Gelatin Capsule 1 Formulation Formulation Amount 1. DHA-containing fish oil (DHA 40%) 60.0 parts Ginkgo biloba extract 15.0 3. 3. soy lecithin (phosphatidylcholine 25%) 2.0 4. Tocopherol-containing vegetable oil extract (tocopherol 85%) 4.0 Beeswax 14.0 6. Glycerin fatty acid ester 5.0 [Preparation method] The above components were stirred and mixed to make uniform, and coated with gelatin to obtain soft capsules having a capsule content of 360 mg.

Comparative Example 1 Gelatin Capsule A (Gelatin Capsule without Lecithin and Tocopherol) A gelatin capsule A was prepared by replacing the soybean lecithin and tocopherol-containing vegetable oil extract with soybean oil in Example 1.

Example 2 Gelatin Capsule 2 Formulation Formulation Amount 1. DHA-containing fish oil (DHA 70%) 42.0 parts Ginkgo biloba extract 5.0 3. Fractionated lecithin (phosphatidylcholine 70%) 1.4 4. Tocopherol 5.0 5. Soybean oil 27.6 6. Beeswax 14.0 7. Glycerin fatty acid ester 5.0 [Preparation method] The above components were stirred and mixed to make uniform, and coated with gelatin to obtain soft capsules having a capsule content of 360 mg.

Comparative Example 2 Gelatin Capsule B (Gelatin Capsule without DHA and Ginkgo Leaf Extract) A gelatin capsule B was prepared by replacing the DHA-containing fish oil and ginkgo leaf extract with soybean oil in Example 2.

Example 3 Gelatin Capsule 3 Formulation Formulation Amount DHA 20.0 parts2. Ginkgo biloba extract 10.0 3. Phosphatidylcholine 0.14. 4. Tocopherol-containing vegetable oil extract (tocopherol 20%) 5.0 Soybean oil 45.9 6. Beeswax 14.0 7. Glycerin fatty acid ester 5.0 [Preparation method] The above components were stirred and mixed to make uniform, and coated with gelatin to obtain soft capsules having a capsule content of 360 mg.

Example 4 Granules Formulation Formulation Amount 1. DHA-containing fish oil (DHA 70%) 30.0 parts 2. Yolk lecithin (phosphatidylcholine 50%) 1.0 3. Tocopherol-containing vegetable oil extract (tocopherol 85%) 2.0 4. Porous enzyme-modified dextrin 33.0 Ginkgo biloba extract 10.0 6. Lactose 20.0 7. Pullulan 4.0 [Production Method] Add components 1 to 3 to component 4 and mix by stirring to obtain a powder. Next, components 5 and 6 are added, and the mixture is dried while spraying the component 7 with a fluidized bed granulator to obtain granules.

Example 5 Dressing Formulation Amount 1. 1. DHA-containing fish oil (DHA 20%) 50.0 parts Olive oil 23.5 3. 3. Tocopherol-containing vegetable oil extract (tocopherol 20%) 5.0 Soy lecithin (phosphatidylcholine 20%) 0.55. Vinegar 10.0 6. Ginkgo biloba extract 5.0 7. Salt 1.08. Mustard 5.0 [Preparation] Add components 5 to 8 to components 1 to 4 and fill the mixture with filter paper while stirring to obtain a dressing.

Example 6 Cooking Oil Formulation Amount 1. 1. DHA-containing fish oil (DHA 40%) 37.5 2. Lard 30.0 3. Sesame oil 11.5 4. Tocopherol 5.0 5. Fractionated lecithin (phosphatidylcholine 70%) 1.0 6. Ginkgo biloba extract 15.0 [Preparation method] After heating components 1 to 5 to make them uniform, add component 6, stir and mix, and pack and cool to obtain cooking oil.

[0022]

According to the present invention, it is possible to provide a food excellent in improving learning and memory ability. Hereinafter, the effects of the present invention will be described using experimental examples.

EXPERIMENTAL EXAMPLE 1 Method: The test of the effect of improving learning and memory was conducted using a paper (Morris. RGB:
Learn Motiv. 1981: 12: 238-260). That is, of the capsules containing DHA + Ginkgo biloba extract + Lecithin + Tocopherol (Example 1), Ginkgo biloba extract + DHA (Comparative Example 1), and Lecithin + Tocopherol (Comparative Example 2), only the contents from which the capsules have been removed To an 8-month-old mouse for 10 days at 1 mg / day
(Suspended in 0.5% carboxymethylcellulose) orally. On the day of administration, the mice are allowed to swim in a water maze, and after learning the position of the platform (exit), they are allowed to swim again 24 hours later, and the time to reach the exit is measured. Mice with improved learning and memory ability reach the exit 24 hours later.

Results: In Comparative Examples 1 and 2, the learning and memory ability was improved. Furthermore, in Example 1, an improvement effect higher than Comparative Examples 1 and 2 was obtained, and a synergistic effect of DHA + Ginkgo biloba extract + Lecithin + Tocopherol was recognized.

[0025]

FIG.

Experimental Example 2 Method: The capsules of Example 1 and Comparative Examples 1 and 2 were added at a rate of 3 capsules per day.
10 adults 10 capsules (1080 mg)
Feed for days. Next, a three-digit / two-digit division was calculated within a determined time, and the number of replies and the correct answer rate before and after the test were examined to test the effect of improving the thinking ability.

Results: In Comparative Examples 1 and 2, both the number of replies and the number of correct answers showed an improvement effect. In Example 1, both the number of responses and the number of correct answers were higher than those of Comparative Examples 1 and 2, and the effect was improved by using DHA + Ginkgo biloba extract + Lecithin + Tocopherol in combination.

[0028]

[Table 1]

Tests were performed on Examples 2 and 3 in the same manner as in Experimental Example 1. As a result, the same effect of improving learning and memory ability as in Experimental Example 1 was observed. In addition, tests were performed on Examples 4, 5, and 6 in the same manner as in Experimental Example 2.
The same effect of improving learning and memory ability was observed.

[0030]

[Brief description of the drawings]

FIG. 1 is a diagram showing the results of improving learning and memory ability using mice by using DHA, ginkgo biloba extract, lecithin and tocopherol according to the present invention in combination.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 31/685 A61K 31/685 35/78 35/78 W (72) Inventor Yutaka Iwata Tomicho, Nishi-ku, Nagoya 2-7 Japan Menard Cosmetics Co., Ltd. F-term (reference) 4B018 LB01 LB08 LB09 LE02 MD11 MD26 MD46 MD48 ME14 MF01 4B035 LC06 LE01 LE03 LG07 LG37 LP22 4C086 AA01 AA02 DA14 DA34 EA11 GA17 MA03 MA04 MA09 NA05 ZA15 ZC75 AC05 BA08 BA14 CA03 MA52 NA05 ZA15 ZC75 4C206 AA01 AA02 DA05 KA04 MA03 MA04 MA72 NA05 ZA15 ZC75

Claims (3)

[Claims] 1. A learning and memory improving food containing docosahexaenoic acid, ginkgo biloba extract, lecithin and tocopherol. 2. The weight composition ratio of the above components is docosahexaenoic acid content / ginkgo leaf extract content = 1 to 1.
6 Amount of tocopherol / amount of phosphatidylcholine in lecithin = 1-50 (tocopherol + phosphatidylcholine in lecithin) / (docosahexaenoic acid + ginkgo biloba extract)
The learning / memory ability improving food according to claim 1, which satisfies all the requirements of the amount of 0.022 to 0.24. 3. The learning / memory improving food according to claim 1, wherein the ginkgo biloba leaf extract is a flavone glycoside.