JP2000206040A - SPF evaluation method and evaluation device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method and an apparatus in which an SPF value can be evaluated quickly, at low costs and objectively by a method wherein a sample injection process is provided, a spectrum measuring process is provided, a calculation process is provided and the SPF value is calculated by using a specific expression. SOLUTION: A light source 10 radiates ultraviolet rays L1 at a wavelength of 290 to 400 nm. A spectroscope 12 selects a desired wavelength λ of the ultraviolet rays L1 radiated from the light source 10. A sample cell 14 is installed in the sample chamber 24 of a spectrophotometer in such a way that a dilution sample 22 is irradiated with ultraviolet rays L2 from the spectroscope 12. A CPU 34 accesses a program storage part 26 so as to read out a specific expression, it accesses a storage parts 28, 30 and 32 so as to read out respective data, and it claculates the SPF equivalent value of the dilution sample 22. The ultraviolet rays L2 which are incident on the dilution sample 22 and which are radiated from the spectroscope 12 are reflected and transmitted to all directions. Since most of diffused and transmitted light L3 can be taken into by an integrating sphere 16, the SPF equivalent value can be measured more precisely. In addition, when the sample is measured by using the spectrophotometer, many samples can be tested quickly and at low costs as compared with conventional cases.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an SPF evaluation method and an evaluation apparatus, and more particularly to an SPF measurement method using a spectrophotometer.

[0002]

2. Description of the Related Art Ultraviolet rays contained in sunlight are long-wavelength ultraviolet rays (UV rays) from 400 to 320 nm.
A), medium wavelength ultraviolet light (UV) from 320 nm to 290 nm
B) It is classified into short wavelength ultraviolet (UVC) of 290 nm or less. Among them, ultraviolet light having a wavelength of 290 nm or less
It is absorbed by the ozone layer and does not reach the surface. Ultraviolet rays reaching the earth's surface have various effects on human skin. Among the ultraviolet rays that reach the surface of the earth, UVB forms erythema and blisters on the skin and also promotes melanogenesis.

[0003] On the other hand, UVA causes browning of the coating, promotes a decrease in skin elasticity and the occurrence of wrinkles, and causes rapid aging. It may also accelerate the onset of the erythema reaction, or enhance it for certain patients, and even cause phototoxic or photoenergy responses. In order to protect the skin from such harmful effects of ultraviolet rays, various ultraviolet absorbers have been developed and are used in many cosmetics. An SPF (Sun Prot) is used as an index indicating the UV-blocking effect of the UV-absorbing skin cosmetic in the UVB region.
ect Factor) values are known.

Conventionally, a living body test by a subject is generally used as an SPF evaluation method. For example, 10 or more healthy subjects are selected, and a sample is applied to the test site.
Sunbathe for 0 to 45 minutes. Sixteen to twenty-four hours after that, a minimum amount of ultraviolet light (hereinafter referred to as MED) at which slight erythema is observed in almost the entire illuminated field of the sample-coated portion and the sample-non-coated portion is visually determined by a skilled person.

[0005] Then, the SPF value is calculated according to the following equation using the MED obtained in the sample non-coated portion and the sample coated portion. SPF value = MED sample applied portion / MED sample non-applied portion The SPF value of this sample was taken as the arithmetic average of the SPF values of each subject thus obtained.

[0006]

The conventional SP
In the F evaluation method, generally, the more the number of subjects, the more reliable the SPF value can be obtained. However, the conventional S
When a living body test is performed by a subject as in the PF evaluation method, it takes a certain amount of time and effort to obtain a result. In addition, due to various restrictions such as labor costs for the subject, it was not possible to manage a satisfactory number of tests, and there was still room for improvement in performing an objective SPF evaluation.

Thus, in this type of field, although there has been a strong demand for the development of an alternative method for the above-mentioned biological test using an analytical instrument or the like, there has not been any technique that can solve this. SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned problems of the related art, and has as its object to provide an SPF capable of evaluating an SPF value more quickly, at low cost, and objectively.
It is to provide a PF evaluation method and an evaluation device.

[0008]

In order to achieve the above object, an SPF evaluation method according to the present invention comprises a sample injection step,
A spectrum measuring step and a calculating step are provided. In the sample injecting step, a diluted sample obtained by diluting a sample with an ultraviolet transmitting diluent so as to have a predetermined sample concentration is put into a sample cell made of an ultraviolet transmitting material. In the spectrum measuring step, the sample cell is inserted into an optical path of a spectrophotometer to obtain a diffuse transmission spectrum T (λ) having a predetermined measurement wavelength range and a predetermined spectral bandwidth in the UVB and / or UBA region.

In the calculating step, an SPF equivalent value is calculated according to the following equation using the diffuse transmission spectrum T (λ). SPF equivalent value = (ΣE (λ) · R (λ)) / (ΣT (λ) · E (λ) · R (λ)) ………………………………………. Here, E (λ) is the energy distribution of the light source of the spectrophotometer, and R (λ) is the relative erythema action spectrum.

[0010] As the ultraviolet transmitting diluent of the present invention, for example, ethyl acetate, silicone oil and the like can be used. In the sample injection method of the present invention, a sample is diluted with the ultraviolet transmitting diluent so as to have a sample concentration of, for example, 2 mg / cm ² or 2 μl / cm ² .
A method in which a predetermined amount is weighed with a syringe and injected into a sample cell is given as an example.

As the sample cell of the present invention, for example, a quartz cell or the like can be used. Table 1 and the like are given as examples of the energy distribution E (λ) of the light source of the spectrophotometer of the present invention.

[0012]

[Table 1] ―――――――――――――――――――――――――――――――――― Wavelength (nm) Spectral irradiance (W.m^-2.nm^-1) ―――――――――――――――――――――――――――――――――――― 290 0.157 × 10^-4  295 0.134 × 10^-2  300 0.136 × 10^-1  305 0.767 × 10^-1  310 0.172 315 0.282 320 0.375 325 0.494 330 0.629 335 0.602 340 0.675 345 0.650 350 0.692 355 0.743 360 0.674 365 0.849 370 0 .876 375 0.780 380 0.902 385 0.693 390 0.897 395 0.693 400 1.180 ―――――――――――――――――――――――― ――――――――――――

The relative erythema action spectrum R of the present invention
(Λ) may be slightly different from that of Japanese skin, for example, because it is obtained from white skin of Australia, New Zealand, etc., but Australia / New Zealand Standards, published in 1996,
Sun protection coating-evaluation and classification [Australian / New Zea
lamd Standards Sun protective clothing-Evalution a
nd classification] (AS / NZS 4399: 1996), Standards Australia / Standards New Zealand [STA
Table 2 described in [NDARDS AUSTRALIA / STANDARDS NEW ZEALAND] is given as an example.

[0014]

[Table 2] CIE erythema action (λ) [CIE ERYTHEMAL EFFECTIVENESS (λ)] ―――――――――――――――――――――――――――――――― ―――― Wavelength, nm Relative spectral effectiveness ――――――――――――――――――――――――――――――――――― -290 1.0000 295 1.0000 300 0.649 305 0.220 310 0.745E-01 315 0.252E-01 320 0.860E-02 325 0.290E-02 330 0.136E-02 335 0. 115E-02 340 0.970E-03 345 0.810E-03 350 0.680E-03 355 0.580E-03 360 0.480E-03 365 0.410E-03 370 0.340E-03 375 0.290E -03 380 0.243E-03 385 0.204E-03 390 0.172E-03 395 0.145E-03 400 0.122E-03 ―――――――――――――――――― ――――――――――――――――――

However, in Table 2 above, reference was made to the standard action spectrum of ultraviolet light in which erythema was observed on human skin described in CIE Research Note, CIE J 6: 17-22, 1987.
Further, an SPF evaluation method according to the present invention includes a sample application step, a spectrum measurement step, and a calculation step.

In the sample applying step, the sample is applied as it is to a sample holding plate made of an ultraviolet transmitting material so as to have a predetermined sample application amount. In the spectrum measuring step, the sample cell is inserted into an optical path of a spectrophotometer to obtain a diffuse transmission spectrum T (λ) having a predetermined measurement wavelength range and a predetermined spectral bandwidth in the UVB and / or UBA region. The calculating step calculates an SPF equivalent value according to the above equation (1) using the diffuse transmission spectrum T (λ).

In the method of applying a sample according to the present invention, the step is 2
There are a method of applying a spatula to a quartz plate having a concave surface processed to 0 μm, a method of extending a sample to a flat quartz glass plate with a spatula processed to a step of 20 μm, and a method of applying the sample to quartz glass with a spinner. As the sample holding plate of the present invention,
For example, a quartz plate or the like can be used. In order to achieve the above object, an SPF evaluation device according to the present invention includes a light source, a spectroscope, a sample cell, an integrating sphere, a detector, a spectroscope control unit, and a calculation unit. It is characterized by having.

The light source emits UVB and / or UVA. The spectroscope selects a desired wavelength λ of ultraviolet light emitted from the light source. The sample cell is filled with a diluted sample obtained by diluting a sample with an ultraviolet transmitting diluent so as to have a predetermined sample concentration, and is provided with an ultraviolet transmitting material provided so that ultraviolet light from the spectroscope is applied to the diluted sample. Consists of

The integrating sphere collects the diffuse transmitted light from the diluted sample. The detector detects the amount of diffuse transmitted light collected by the integrating sphere. The spectroscope control means controls the operation of the spectroscope so as to obtain a diffuse transmission spectrum having a predetermined measurement wavelength range and a predetermined spectral bandwidth in the UVB and / or UVA region.

The calculating means uses the diffuse transmission spectrum T (λ) to calculate an SPF equivalent value according to the above equation (1). Further, an SPF evaluation apparatus according to the present invention includes a light source, a spectroscope, a sample holding plate, an integrating sphere, a detector, a spectroscope control unit, and a calculation unit. The light source emits UVB and / or UVA.
The spectroscope selects a desired wavelength λ of ultraviolet light emitted from the light source.

The sample holding plate is made of an ultraviolet transmitting material which is provided so that the sample is applied as it is so as to have a predetermined sample application amount and the sample is irradiated with ultraviolet rays from the spectroscope. The integrating sphere collects diffuse transmitted light from the sample. The detector detects the amount of diffuse transmitted light collected by the integrating sphere.

The spectroscope control means controls the operation of the spectroscope so as to obtain a diffuse transmission spectrum having a predetermined measurement wavelength range and a spectrum bandwidth in the UVB and / or UVA region. The calculating means uses the diffuse transmission spectrum T (λ) to calculate S
Calculate the PF equivalent value.

The term "diffuse transmission spectrum T (λ)" used herein refers to, for example, the diffuse transmission light rate with a sample inserted therein relative to the total transmission light rate from a sample cell or a sample holding plate without a sample. In the UVB and / or UVA region, it refers to a value measured for each measurement wavelength λ having a predetermined measurement wavelength range and a spectrum bandwidth. In addition, the energy distribution of the light source referred to here is, for example, a UV radiation intensity distribution of a lamp obtained in advance using an ultraviolet intensity meter or the like.
Among the B and / or UVA regions, it refers to those taken for each measurement wavelength λ having a predetermined measurement wavelength range and a spectral bandwidth.

In addition, the relative erythema action spectrum referred to herein means, for example, the distribution of the sample-coated part MED / sample-non-coated part MED obtained in advance, which is measured in a predetermined measurement wavelength range in the UVB and / or UVA region. And for each measurement wavelength λ having a spectral bandwidth.

[0025]

BRIEF DESCRIPTION OF THE DRAWINGS FIG.
A simple embodiment will be described.First embodiment  FIG. 1 shows an SPF evaluation device according to the first embodiment of the present invention.
A schematic configuration is shown. In this embodiment,
Describes an example in which a diluted sample obtained by diluting a sample is placed in a sample cell.
And insert it into the optical path of the spectrophotometer.
The case of measuring the diffuse transmission spectrum of
You.

The SPF evaluation device shown in FIG.
, A spectrometer 12, a sample cell 14, an integrating sphere 16, a detector 18, a calculating means 20, and a spectrometer controlling means.
The light source 10 includes, for example, a xenon arc solar simulator and emits ultraviolet light L1 having a wavelength of 290 to 400 nm. The spectroscope 12 selects a desired wavelength λ of the ultraviolet light L1 emitted from the light source 10.

The sample cell 14 is made of, for example, a quartz cell (optical path length 1 to 10 mm) made of an ultraviolet transmitting material and the like, and a sample such as a sunscreen claim is made of ethyl acetate or the like so as to be 0.1 g / 50 ml. A diluted sample 22 diluted with an ultraviolet transmission diluent is placed therein. This sample cell 14
Is installed in the sample chamber 24 of the spectrophotometer so that the ultraviolet light L2 from the spectroscope 12 is irradiated on the diluted sample 22.

The integrating sphere 16 collects the diffuse transmitted light L3 from the diluted sample 22. The detector includes, for example, a photomultiplier tube and the like, and detects the amount of diffuse transmitted light L3 collected by the integrating sphere 16. The calculating unit 20 includes, for example, a program storage unit 26, a diffuse transmission spectrum data storage unit 28, and a light source energy distribution data storage unit 30.
And a relative erythema action spectrum data storage unit 32, and a CPU 34 for performing desired arithmetic processing.

The program storage unit 26 stores, for example, the above equation (1) necessary for calculating the SPF equivalent value of the diluted sample 22. The diffuse transmission spectrum data storage unit 28 stores, for the diluted sample 22, diffuse transmission spectrum data having a measurement wavelength range of 290 to 400 nm and a spectral bandwidth of 5 nm. The light source energy distribution data storage unit 30 stores the energy distribution data of the lamp (not shown) of the light source 10 obtained in advance in the wavelength range 29.
It stores a measurement wavelength λ having a wavelength of 0 to 400 nm and a spectral bandwidth of 5 nm.

The relative erythema action spectrum data storage unit 32 stores the previously obtained relative erythema action spectrum data for a measurement wavelength range 290 to 400 nm and a measurement wavelength λ having a spectral bandwidth of 5 nm.
The CPU 34 performs Fourier transform on the interferogram data from the detector 18 to obtain spectrum data. The obtained spectrum data is stored in the diffuse transmission spectrum data storage unit 28.

The CPU 34 accesses the program storage unit 26, reads out the above equation (1), and according to the read out equation (1), the data storage units 28, 30, 3
2 is accessed, the diffuse transmission spectrum data, the light source energy distribution data, and the relative red erythema action spectrum data are read, and the SPF equivalent value of the diluted sample 22 is calculated. The spectroscope control means includes, for example, a drive circuit 36 and a control circuit including, for example, the CPU 34 and the like.
The wavelength λ selected by the spectroscope 12 is controlled so that diffuse transmission spectrum data having a wavelength range of m and a spectral bandwidth of 5 nm is obtained.

The SPF evaluation apparatus according to this embodiment is configured as described above, and its operation will be described below. First, as shown in FIG. 2, the sample is diluted and injected into the sample cell (step 10). That is, the sample is diluted with an ultraviolet transmission diluent so as to have a sample concentration of 2 mg / cm ² or 2 μl / cm ² . A predetermined amount of the diluted sample 22 thus obtained is weighed with a syringe, and the sample cell 14
Inject into After injection, the spectral bandwidth was 5 nm, 29
For the measurement wavelength λ in the measurement wavelength range of 0 nm to 400 nm, the diffuse transmittance of the diluted sample 22 is obtained, and the diffuse transmission spectrum is obtained (step 12).

First, the sample cell 14 in a state where the diluted sample 22 is not injected is set in the sample chamber 24 of the spectrophotometer.
Total transmitted light intensity data is obtained for each measurement wavelength λ in the measurement wavelength range of 90 to 400 nm. Next, the diluted sample 22
Is set in the sample chamber 24 of the spectrophotometer, and from the output of the detector 18, the diffuse transmission is performed for the 5 nm spectral bandwidth and each measurement wavelength λ in the 290 to 400 nm measurement wavelength range. Obtain light intensity data.

The CPU 34 obtains a diffuse transmission spectrum of the diluted sample 22 from the ratio of these intensity data. FIG. 3 shows an example of diffuse transmission spectra of some diluted samples having different amounts of the ultraviolet absorber. In the figure,
The diluted sample 22 is 0.1 g / 50 ml (ethyl acetate solution or the like), the sample cell 14 has an optical path length of 1 mm, D6 has an SPF value of 6 based on human body experiments, and D16 has SP based on human body experiments.
The F value is 16, D50 is an SPF value obtained from a human body experiment of 50, and D98 is an SPF value obtained from a human body experiment is 98.

Here, the spectroscope 1 incident on the diluted sample 22
Although the ultraviolet rays L2 from 2 are reflected and transmitted in all directions, in the present embodiment, most of the diffused transmitted light L3 can be taken in by the integrating sphere 16, so that more accurate measurement is possible. Further, in the present embodiment, by measuring a sample using a spectrophotometer, a large number of samples can be tested quickly and at low cost as compared with the case where a conventional biological test is used.

After the measurement, an SPF equivalent value is calculated (step 14). That is, the CPU 34 controls each data storage unit 2
8, 30, and 32, the diffuse transmission spectrum data, the light source energy distribution data, and the relative erythema reflex action spectrum data are read, and using the read data,
The SPF equivalent value of the sample is determined according to the equation (1).

For example, a measurement wavelength range of 290 nm to 400
nm and a spectral bandwidth of 5 nm, the CPU 3
4, the above equation (1) is equivalent to SPF = (E (290) × R (290) + E (2
95) × R (295) +... + E (395) × R (39
5) + E (400) × R (400)) / (T (290)
× E (290) × R (290) + T (295) × E (2
95) × R (295) +... + T (395) × E (39
5) × R (395) + T (400) × E (400) × R
(400)) is calculated.

After the calculation, the SPF equivalent value is evaluated. That is, the CPU 34 plots the SPF value of the standard sample on the abscissa and the SPF equivalent value obtained by the SPF evaluation device according to the present embodiment on the abscissa, and linearly interpolates between the plots to obtain a calibration curve. This is shown in FIG.

The figure shows a correlation of 0.9 or more, and the equivalent value obtained by the SPF evaluation apparatus according to the present embodiment shows that a result highly correlated with the SPF value of the standard sample is obtained. It is shown. As described above, in the present embodiment, by measuring a sample using a spectrophotometer, a large number of samples can be tested quickly and at low cost as compared with a case where a biological test is used. Objective SPF evaluation with an analytical instrument, which has been difficult, becomes possible.

As described above, according to the SPF evaluation apparatus of the present embodiment, the diluted sample 22 obtained by diluting the sample with the ultraviolet transmitting agent is put into the sample cell 14 and the sample chamber 24 of the spectrophotometer is set.
And the diffuse transmission spectrum of the wavelength in the UVB and UVA regions was measured, and the SPF equivalent value was determined according to the above equation (1) using the obtained diffuse transmission spectrum, which was conventionally extremely difficult. In addition, the SPF measurement of multiple samples can be performed quickly and at low cost. As a result, in the present embodiment, objective SPF evaluation with an analytical instrument, which has been extremely difficult in the past, becomes possible.

[0041]Second embodiment  FIG. 5 shows an SPF evaluation device according to a second embodiment of the present invention.
Is schematically shown. Note that this corresponds to FIG.
The parts are indicated by the reference numeral 100 and the description is omitted. same
In the SPF evaluation device shown in the figure, the sample application amount is 2 mg / c
m²Or 2 μl / cm²The sample 138 is
Sample holder made of an ultraviolet transmitting material such as a quartz plate
It is applied to the holding plate 140.

For example, when the sample application area is set to 20 cm ² , 0.04 ml of the emulsion is measured with a syringe and spread on the surface of the sample holding plate 138. It is applied with a fingertip so as to be uniform within a certain area. Here, the step is 20 μ
There are also a method of applying a spatula to a quartz plate having a concave surface processed to m, a method of spreading a sample on a flat quartz glass plate with a spatula processed to a step of 20 μm, and a method of applying the sample to quartz glass with a spinner. The sample holding plate 140 coated with the sample 138 in this manner is inserted into the sample chamber 124 of the spectrophotometer, and the diffuse transmission spectrum T (λ) is obtained in the same manner as in the first embodiment.

The SPF evaluation apparatus according to this embodiment is configured as described above, and its operation will be described below. FIG. 6 is a flowchart showing the processing contents of the apparatus shown in FIG. Note that portions corresponding to those in FIG. 2 are denoted by reference numeral 100, and description thereof is omitted. First,
A sample is applied (step 100).

For example, the sample 138 is applied to the sample holding plate 140 as it is so that the sample application amount is 2 mg / cm ² or 2 μl / cm ² ,
In the case of cm ² , 0.04 ml of the emulsion was measured with a syringe and poured on the surface of the sample holding plate. It is applied with a fingertip so that it is uniform within a certain area with a mark. Here, a method of applying a spatula to a quartz plate with a concave surface with a step processed to 20 μm, a method of spreading a sample on a flat quartz glass plate with a spatula processed to a step of 20 μm, and coating on quartz glass with a spinner There is also a way to do it.

As described above, in this embodiment, the sample 138 is applied to the sample holding plate 140 as it is, so that the state of the sample 138 does not deviate from the state in actual use as much as possible. After the application, the sample holding plate 140 is inserted into the sample chamber 124 of the spectrophotometer,
A diffuse transmission spectrum having a wavelength range of 5 nm and a spectral bandwidth of 5 nm is obtained (step 112). Then, using the obtained diffuse transmission spectrum data, the CPU 134
However, an SPF equivalent value of the sample 138 is obtained according to the above equation (1) (step 114).

As described above, according to the SPF evaluation apparatus according to the present embodiment, the sample 138 is applied as it is to the sample holding plate 140 and inserted into the sample chamber 124 of the spectrophotometer.
Since the diffuse transmission spectrum at a wavelength of 0 nm to 400 nm is measured, and the obtained diffuse transmission spectrum is used to determine the SPF equivalent value according to the above equation (1), it is extremely difficult in the past, as in the first embodiment. Can be quickly and at low cost.

As a result, in the present embodiment, as in the first embodiment, objective SPF evaluation with an analytical instrument, which has been extremely difficult in the past, becomes possible. Moreover, in the present embodiment, the sample 138 is not diluted and the sample holding plate 14 is not diluted.
0, and the diffuse transmission spectrum is measured so that the state of the sample 138 does not deviate from the state in actual use as much as possible in comparison with the first embodiment. As a result, compared to the case where the sample 138 is diluted or the like, it is possible to measure the ultraviolet ray prevention effect closer to the actual use state.

In each of the above embodiments, the energy distribution E (λ) of the light source and the relative erythema action spectrum R (λ)
Has been described using an example obtained beforehand before obtaining the diffuse transmission spectrum T (λ) of the sample. However, the present invention is not limited to this.
It may be obtained at the time of (λ) measurement, after measurement, or the like.

[0049]

As described above, the SP according to the present invention is used.
According to the F evaluation method and the evaluation apparatus, the sample is diluted and put into the sample cell, or the sample is directly applied to the sample holding plate,
Since it was inserted into the optical path of the spectrophotometer, the diffuse transmission spectrum in the UVB and / or UVA region was measured, and the obtained diffuse transmission spectrum was used to determine the SPF equivalent value according to the above equation (1). It becomes possible to perform SPF measurement of many samples, which was extremely difficult, quickly and at low cost. This enables objective SPF evaluation with an analytical instrument, which has been extremely difficult in the past.

[Brief description of the drawings]

FIG. 1 is an explanatory diagram of a schematic configuration of an SPF evaluation device according to a first embodiment of the present invention.

FIG. 2 is a flowchart showing processing contents of the apparatus shown in FIG. 1;

FIG. 3 is an example of a transmission spectrum of a diluted sample.

FIG. 4 is an explanatory diagram of a correlation between an SPF value obtained in a human body test and a numerical value obtained in the present invention. .

FIG. 5 is an explanatory diagram of a schematic configuration of an SPF evaluation device according to a second embodiment of the present invention.

FIG. 6 is a flowchart showing processing contents of the apparatus shown in FIG. 5;

[Explanation of symbols]

 10, 110: Light source 12, 112: Spectroscope 14: Sample cell 16, 116: Integrating sphere 18, 118: Detector 20, 120: Calculation means 140: Sample holding plate

Claims (4)

[Claims] 1. A sample injection step in which a diluted sample obtained by diluting a sample with an ultraviolet transmitting diluent so as to have a predetermined sample concentration is placed in a sample cell made of an ultraviolet transmitting material, and the sample cell is placed in an optical path of a spectrophotometer. Insert, UVB and / or UBA
A spectrum measuring step of obtaining a diffuse transmission spectrum T (λ) of a predetermined measurement wavelength range and a spectral bandwidth in the region; and calculating using the diffuse transmission spectrum T (λ) to calculate an SPF equivalent value according to the following equation. And a step, wherein the SPF equivalent value is (ΣE (λ) · R
(Λ)) / (ΣT (λ) · E (λ) · R (λ)), where E (λ) is the energy distribution of the light source of the spectrophotometer, and R (λ) is An SPF evaluation method characterized by a relative erythema action spectrum. 2. A sample application step of applying a sample as it is to a sample holding plate made of an ultraviolet transmitting material so as to have a predetermined sample application amount, and inserting the sample cell into an optical path of a spectrophotometer, Or a spectrum measurement step of obtaining a diffuse transmission spectrum T (λ) of a predetermined measurement wavelength range and a spectrum bandwidth in the UBA region, and using the diffuse transmission spectrum T (λ) to calculate an SPF equivalent value according to the following equation: Calculating step, wherein the SP
The value corresponding to F is (ΣE (λ) · R (λ)) / (ΣT (λ)
.E (λ) .R (λ)), where E
(Λ) is the energy distribution of the light source of the spectrophotometer;
(Λ) is a relative erythema spectrum, the SPF evaluation method. 3. A light source that emits UVB and / or UVA, a spectroscope that selects a desired wavelength λ of ultraviolet light emitted from the light source, and a sample that is irradiated with an ultraviolet light-transmitting diluent so as to have a predetermined sample concentration. A sample cell made of an ultraviolet-transmissive material in which a diluted sample is placed and which is irradiated with ultraviolet light from the spectroscope onto the diluted sample; and an integrating sphere for condensing diffuse transmitted light from the diluted sample. And a detector for detecting the amount of diffuse transmitted light condensed by the integrating sphere, and a diffuse transmission spectrum T (λ) having a predetermined measurement wavelength range and a predetermined spectral bandwidth in the UVB and / or UVA regions. And a calculating means for calculating an SPF equivalent value using the diffuse transmission spectrum T (λ) according to the following equation using the diffuse transmission spectrum T (λ).
The value corresponding to F is (ΣE (λ) · R (λ)) / (ΣT (λ)
.E (λ) .R (λ)), where E
(Λ) is an energy distribution of the light source, and R (λ) is a relative erythema action spectrum. 4. A light source that emits UVB and / or UVA, a spectroscope that selects a desired wavelength λ of ultraviolet light emitted from the light source, and a sample is applied as it is so as to have a predetermined sample application amount. A sample holding plate made of an ultraviolet transmitting material placed so that the sample is irradiated with ultraviolet rays from the spectroscope, an integrating sphere that collects diffuse transmitted light from the sample, and a light that is collected by the integrating sphere. And a detector for detecting the amount of diffused transmitted light, and the operation of the spectrometer so as to obtain a diffused transmission spectrum T (λ) having a predetermined measurement wavelength range and a spectral bandwidth in the UVB and / or UVA regions. And SP calculating means for calculating an SPF equivalent value using the diffuse transmission spectrum T (λ) according to the following equation:
The value corresponding to F is (ΣE (λ) · R (λ)) / (ΣT (λ)
.E (λ) .R (λ)), where E
(Λ) is an energy distribution of the light source, and R (λ) is a relative erythema action spectrum.