JP2000178198A - Cell activator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain cell-activating agent that shows excellent stability, safety and cell-activating effect and has excellent preventive and improving effect for skin symptoms as drying, deterioration in skin elasticity as well as anti- inflammatory effect and vulnerary effect by using the plants in Adenophora and Clerodendron trichotomum or their extracts. SOLUTION: This cell activator contains plants in Adenophora (Adenophora triphylla, Adenophora tetraphylla, Adenophora axillifloa, Adenophora pereskiaefolia, Adenophora capillaris, Adenophora bulleyana, Adenophora potaninii, Adenophora polyantha, and Adenophora lilifolioides), particularly Adenophora triphylla, and their extracts and particularly Clerodendron trichotomum or one or two or more kinds of their extracts. The stems, branches, leaves, roots, seeds, fruits, flowers and the like can be used, the extract may be its essence or its isolated and purified products. The amounts of these plants or their extracts are preferably 0.00001-20 wt.%, and more preferably 0.0001-10 wt.%.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cell activator which is suitably used for pharmaceuticals, quasi-drugs, cosmetics, etc. and has high safety and high cell activating effect.

[0002]

BACKGROUND OF THE INVENTION Usually,
Young cells are actively metabolizing, and can quickly improve functions such as deterioration caused by various factors. However, with aging changes, a decrease in cell activity occurs, which causes various symptoms. In skin cells, a decrease in epidermal and dermal cell activity occurs, which causes skin symptoms such as dryness and reduced elasticity. Metabolism is also reduced in hair matrix cells, and symptoms such as hair loss and thinning occur. Further, in the oral tissues, the activity of gingival fibroblasts is reduced, the gingival regression and the immunity are reduced, the morbidity of gingival pathogens is increased, and periodontal disease progresses. With respect to such senescent cells, search for cell activators has been conducted in order to activate these cells themselves and improve various aging symptoms.

[0003] Examples of such substances include bovine placenta extract (JP-A-3-141299), whey extracts, crude drug extracts such as hormones, vitamins, saponins and the like, and olive extract as a plant component (JP-A-Hei. No. 7-138179) and extracts such as sesame, sanyak, and pepper (Japanese Patent Application Laid-Open No. Hei 10-45615). -There have been problems such as insufficient effect and difficulty in stably compounding the preparation while maintaining the activity.

The present invention has been made in view of the above circumstances, and provides a cell activator which can be applied to various fields such as pharmaceuticals, quasi-drugs, and cosmetics, is excellent in safety and has an excellent cell activating effect. The purpose is to provide.

[0005]

Means for Solving the Problems and Embodiments of the Invention The present inventors have conducted intensive studies on the cell activating effect of a natural product, and as a result, it has been found that a ginseng (Adenophor) is obtained.
a) Genus, Egret (Clerodendrontrich)
(Otum) and their extracts were found to have an excellent cell activating effect, leading to the present invention. The cell activator of the present invention activates the metabolism of cells and prevents aging of the skin, or is used in combination with various external preparations such as an external preparation for the skin, a preparation for the scalp and the hair, to prevent skin aging. It gives cosmetic effects such as improvement of wounds and wound healing. In addition to external preparations, it is effective in strengthening gingiva and preventing periodontal disease by using it in an oral composition such as dentifrice or mouthwash. .

Hereinafter, the present invention will be described in more detail.
As described above, the cell activator of the present invention includes a plant of the genus Adenophora and a heron (Cle)
(Rendron trichotomum) and an extract thereof, containing one or more as an active ingredient.

[0007] Ginseng (Adenophor)
a) Genus plants are perennials with thick rhizomes, and about 50 species are distributed in Asia and Europe. Egret (Cleroden)
Dron trichotomum is a large deciduous shrub, 2-4 m in height, distributed in Japan.

[0008] Ginseng (Adenophor)
a) Among the genus, in particular, ginseng (Adenop)
hora triphylla var. japon
ica), Rhododendron (Adenophora)
tetraphylla, Sangyo Ansan (Adenoph)
ora axilliflora, ginseng sasan (Ade)
nophora peresciaefolia, Adenophora capillari
s), Yunnan Sasan (Adenophora bullley)
ana), Awashasan (Adenophora potan)
inii), Ishisamsan (Adenophora poly)
antha), Sakura Kawakura (Adenophora li)
Lifoliodes and their extracts have an excellent cell activating effect, and among them, Adenophora triphylla va
r. japonica) and its extract show the highest cell activating effect.

The above-mentioned plant can use its stem (wood, heartwood, bark), branch, leaf, root, seed, fruit, flower and the like.

[0010] The plant extract may be an extract extract or a product separated and purified from the extract. In the case of an extract extract, the plant can be obtained by solvent extraction of a dried or crushed plant,
If the extraction solvent is non-toxic for use, the extract may be used as it is, or may be used as a diluent diluted with an appropriate solvent, or as a concentrated extract, or as a dry powder by freeze-drying, Those prepared in paste form can be used.

Examples of the solvent used to obtain the above plant extract include commonly used organic solvents such as methanol, ethanol, butanol, hexane, heptane, cyclohexane, ethyl acetate, acetone and the like, and water. One of these can be used alone, or two or more can be used in combination. Among these solvents, methanol, ethanol and water are particularly preferred.

[0012] The extraction can be carried out by a conventional method at a temperature of usually about 3 to 70 ° C.

In addition to the solvent extraction, an extract obtained by supercritical extraction in which carbon dioxide is brought into a supercritical state can also be used. At this time, hexane, ethanol, or the like can also be used as an extraction aid.

The separation and purification of the active ingredient from the extract can be carried out by purifying the extract by column chromatography, liquid chromatography and the like.

[0015] The dosage form of the cell activator of the present invention is arbitrary and can be widely used for pharmaceuticals, quasi-drugs, cosmetics, and the like. can do.

Further, a cell activator generally used,
For example, a herbal extract such as hormones, vitamins, and saponins, and a placenta extract can be used in combination.

The amount of the plant or its extract to be added to the cell activator of the present invention varies depending on the dosage form, but is preferably 0.00001 to 20% by weight. Preferably, the content is 0.0001 to 10% by weight.
If the compounding amount is less than 0.00001% by weight, the effects of the present invention cannot be exerted, and the production may be difficult depending on the dosage form. Therefore, it is better not to exceed 20% by weight.

[0018]

EFFECT OF THE INVENTION The cell activator of the present invention is stable, has excellent safety and excellent cell activation effect, and has a preventive / improving effect on skin symptoms such as drying and decrease in elasticity, an anti-inflammatory effect and a wound healing effect. Therefore, the range of application is extremely wide.

[0019]

EXAMPLES The present invention will be specifically described below with reference to Preparation Examples, Examples and Formulation Examples, but the present invention is not limited to the following Examples.

[Preparation Example] Ginseng carrot (Adeno)
phora triphylla var. japon
10 kg (W / V) of methanol was added to 1 kg of cut leaves of ica) leaves, stems and roots, and extracted for 2 hours. Next, the mixture was filtered, and methanol was distilled off under reduced pressure.
Obtained. In a similar manner, an extract of a plant other than the carrot ginseng was obtained.

[Examples] Adenophora plant and a heron (C) obtained according to the preparation examples
Proliferation activity test was carried out using the extract of L. dendron trichotumum) in order to confirm the activation effect on fibroblasts by the following method. In addition, normal human fibroblasts (Kurabo) were used as the fibroblasts. Table 1 shows the results.

Cell culture method Normal human fibroblasts were cultured in a Dulbecco's modified MEM medium containing 10% FBS at 37 ° C., 5% CO ₂ , and humidity of 9%.
The cells were cultured for 4 days under 0% conditions. Thereafter, the medium containing 100 μg / ml of the sample solution or only the medium as a control was replaced, and the cells were further cultured for 2 days.

Propagation using Alamar Blue dye
Activity measurement Cell proliferation measurement dye Alamar Blue (BIOS
OUR INTERNATIONAL) was added to a 1/10 amount of the culture solution, incubated at 37 ° C., 5% CO ₂ , and 90% humidity for 3 hours, and then the fluorescence at an excitation wavelength of 530 nm and a detection wavelength of 590 nm was measured.

[0024]

[Table 1]

As is evident from the results in Table 1, plants of the genus Adenophora and the heron (Clerodendron trichotomu) are shown.
The effect of promoting the proliferation of normal human fibroblasts was remarkably observed in the extract of m). That is, it was proved that the above plant extract had a high cell activating effect. Hereinafter, a formulation example is shown.

[0026]

[Table 2]

[0027]

[Table 3]

[0028]

[Table 4]

[0029]

[Table 5]

[0030]

[Table 6]

[0031]

[Table 7]

[0032]

[Table 8]

[0033]

[Table 9]

[0034]

[Table 10]

[0035]

[Table 11]

[0036]

[Table 12]

[0037]

[Table 13]

[0038]

[Table 14]

[0039]

[Table 15]

[0040]

[Table 16]

[0041]

[Table 17]

[0042]

[Table 18]

[0043]

[Table 19]

[0044]

[Table 20]

[0045]

[Table 21]

[0046]

[Table 22]

[0047]

[Table 23]

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Tatsuo Hayashi 1-3-7, Honjo, Sumida-ku, Tokyo F-Term in Lion Corporation (reference) 4C083 AA082 AA111 AA112 AA122 AB032 AB112 AB172 AB222 AB242 AB442 AC012 AC022 AC072 AC122 AC132 AC182 AC232 AC242 AC352 AC422 AC472 AC482 AC532 AC542 AC582 AC612 AC642 AC692 AC742 AC812 AC852 AC862 AC892 AD022 AD092 AD112 AD132 AD152 AD162 AD282 AD352 AD532 AD532 AD572 AD662 BB60 CC02 CC04 CC05 CC07 CC17 CC23 CC37 CC38 CC39 CC41 DD10 DD23 DD31 EE31 EE11 AB30 AC01 BA07 BA08 NA14 ZA89 ZB22

Claims (1)

[Claims] 1. A cell activator containing one or more species of a plant belonging to the genus Trichinella, a heron or an extract thereof.