JP2000005202A - Animal vascular procedures - Google Patents

Abstract

(57) [Summary] [Object] To provide a method for treating a blood vessel of an animal which can be treated reliably with little damage to the blood vessel. An animal blood vessel treatment method, which comprises using an indwelling needle in an animal blood vessel treatment procedure.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a method for treating a blood vessel in an animal.

[0002]

2. Description of the Related Art In pharmacology tests of pharmaceuticals, metabolism tests of chemical substances, toxicity tests, etc., injection needles are generally used when blood vessels are treated for blood collection, substance administration, blood pressure measurement, etc. Minor movements of the animal or the operator during the procedure can easily cause the needle to fall out of the blood vessel or the needle tip to damage the blood vessel wall, so that re-operation is often required. In addition, during the treatment using the injection needle, the injection needle must be held by hand, and the treatment needs to be completed in a short time. On this occasion,
Since it is necessary to use a thin needle for a thin blood vessel, the amount of blood collected and the administration speed are limited, and when a thick blood vessel capable of collecting and administering a larger amount of blood is used, it takes a long time to stop bleeding after the treatment.

[0003]

Accordingly, there has been a long-felt need for the development of a technique for treating a blood vessel of an animal with little damage to the blood vessel and capable of performing a reliable treatment.

[0004]

Under such circumstances, the present inventors have made intensive studies and as a result, by using an indwelling needle in a vascular treatment procedure for animals, it is possible to perform reliable treatment with little damage to blood vessels. Thus, the present invention has been completed. That is, the present invention provides an animal vascular treatment method using an indwelling needle in an animal vascular treatment procedure.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The indwelling needle used in the present invention is a needle comprising a puncture needle part and a catheter part as shown in FIG. 1, and a commercially available one can be used. During vascular treatment, insert the indwelling needle into the blood vessel with the needle inserted into the catheter, then withdraw the needle and place the catheter in the blood vessel, and attach a syringe or tube to the catheter as appropriate. And take action. Such an indwelling needle is characterized in that the tip of a catheter portion to be indwelled in a blood vessel is not sharp as compared with a general injection needle, and it is hard to be detached from the blood vessel due to a long insertion portion into the blood vessel. The size of the indwelling needle used for the vascular treatment may be selected according to the thickness of the blood vessel of the animal to be treated. For example, when treating the saphenous vein or tail vein of a mature common marmoset, 24
A needle of about a gauge can be preferably used.

[0006] Examples of the vascular treatment using the indwelling needle as described above include blood collection, intravenous administration, blood pressure measurement, and the like. To perform blood collection (venous blood) using an indwelling needle, for example, an indwelling needle is inserted into a saphenous vein, a tail vein, or the like of an animal, and then the catheter section is indwelled in a blood vessel, and a syringe is attached thereto, and the blood is collected. Good to collect. For example, when blood is collected from a saphenous vein of a mature common marmoset using a 24-gauge indwelling needle, at least 5 ml of blood can be collected. After the procedure, surgical wound may be wrapped around the site where the indwelling needle was punctured to compress the wound, and placed in a small cage until hemostasis is completed, during which time the operator can also collect blood from another individual . When a general injection needle is used for such blood collection, the amount of blood collected from the saphenous vein or the tail vein is 0.
It is about 2 ml to 1 ml. In addition, if blood is collected from the femoral vein to collect 2 to 4 ml of blood, the needle tip may touch the artery running along with the puncture and cause bleeding. After the treatment, the puncture site is pressed with fingers to stop the bleeding A need arises. At this time, insufficient hemostasis causes blood leakage,
This will put extra stress on the animal. Therefore, by using an indwelling needle for blood collection, it is possible to reliably collect a large amount of blood and to cause less damage to blood vessels and skin, as compared to the case of using a general injection needle, and to reduce damage after treatment. Is also simple.

To perform intravenous administration using an indwelling needle, for example, an indwelling needle is inserted into a saphenous vein, a tail vein, or the like of an appropriately maintained animal, and then the catheter section is indwelled in a blood vessel, and the administration solution is injected into the blood vessel. Administration may be performed with a filled syringe. In addition, long-term administration can be performed by attaching an extension tube to a catheter portion indwelled in a blood vessel, connecting the extension tube to a reflux pump, and setting an appropriate flow rate. During this time, if the indwelling site of the catheter is held with surgical tape, there is no need for the processor to keep holding it by hand. By using an indwelling needle for intravenous administration in this manner, the needle is unlikely to fall out of or penetrate a blood vessel as compared with the case of using a general injection needle, and reliable administration can be performed. In particular, administration of a skin-irritating drug or a compound containing a radioisotope by the method of the present invention can minimize inflammation of the administration site and contamination by the radioisotope caused by leakage of the administration solution. it can.

In order to measure blood pressure using an indwelling needle, for example, an indwelling needle is inserted into a femoral artery or the like of an anesthetized animal, and then a catheter section is indwelled in a blood vessel. By connecting to a measuring device, the arterial pressure can be measured directly. After the measurement, it is preferable to stop bleeding by pressing with fingers. By using an indwelling needle for invasive blood pressure measurement in this way, the skin is dissected under anesthesia, the artery is exposed, cannulation is performed on it, and the blood pressure is measured by connecting to an invasive blood pressure measurement device. Therefore, there is an advantage that postoperative management after the measurement is not required, and the measurement can be repeatedly performed on the same animal.

The vascular treatment method of the present invention can be applied not only to pharmacological tests, metabolic tests, toxicity tests and the like using laboratory animals, but also to treatment and diagnosis of breeding animals, pet animals and the like. The vascular treatment method of the present invention can be applied to various animal species, and in particular, small animals whose blood vessels are thin and difficult to perform vascular treatment with a general injection needle, such as mice, rats, rabbits, small dogs, and small monkeys. It is useful for vascular treatment such as.

[0010]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, but it should not be construed that the present invention is limited thereto. Example 1 Surflow (Termo registered trademark) R indwelling needle in a saphenous vein of a common marmoset of 1 to 6 years old (body weight of 300 to 550 g)
A C-type (24 gauge) was inserted, the catheter portion was placed in the blood vessel, and a syringe (manufactured by Terumo Corporation) was attached to the catheter to collect blood. For comparison, a general injection needle [Neuros injection needle manufactured by Terumo Co., Ltd. (trademark) 26 gauge]
Blood was collected from the same individual using. The blood collected by each method was subjected to hematological tests and blood biochemical tests. With the indwelling needle, 5 ml of blood could be easily collected at one time. Collection of the amount (2-3 ml) required for a normal blood test could be performed very smoothly. When a normal injection needle was used, the amount of blood collected at one time was 0.2 to 1 ml. There was no apparent difference in the results of each test item between blood collected using an indwelling needle and blood collected using a general injection needle.

Example 2 A catheter (Cermo part) was inserted into a saphenous vein of a common marmoset (1 to 6 years old, weighing 300 to 550 g) by inserting a type C (24 gauge) of Surflow (Termo Corporation) R indwelling needle. A syringe filled with the administration solution (manufactured by Terumo Corporation) was attached thereto, and 3 to 5 ml of the administration solution per 1 kg of body weight was injected into the blood vessel (bolus method). Once the needle was indwelled, it was possible to reliably administer without dislodging the needle or piercing the blood vessel with only slight movement of the animal or the operator.

Example 3 A common marmoset of 1 to 6 years old (body weight of 300 to 550 g) was retained using a retainer. Next, a Surflow (Termo Corporation) R indwelling needle type C (24 gauge) is inserted into the saphenous vein to indwell the catheter portion in the blood vessel, and the indwelling site is retained with surgical tape, and the administration solution (saline) ) Was attached thereto, connected to an infusion pump (Syringe Pump, CFV-3200: Nihon Kohden), and the administration solution was injected intravenously over 1 hour (dose volume; 15 ml / kg body weight, administration rate; 0.1 ml / min). 1
The administration was possible without attendant during the time administration.

Example 4 A common marmoset of 1 to 6 years old (body weight of 300 to 550 g) was anesthetized and laid on a warming mat (37 ° C.), and a surflow (Termo Corporation®) R indwelling needle type C (24 gauge) was used. Was inserted into the femoral artery, the catheter portion was placed in the blood vessel, an extension tube and a transducer were attached thereto, and the catheter was connected to a sphygmomanometer (POLYGRAPH SYSTEM: Nihon Kohden). Also,
At the same time, a sensor of a non-invasive automatic blood pressure measurement device (BP-98A: Softlon) was attached to the tail. Blood pressure was measured by intravenously administering isoproterenol (2.0 μg / kg body weight) to the animal, and the measured values by the respective measurement methods were compared (5 males and 5 females per group).
Head, a total of 10). As a control for the substance to be administered, physiological saline (1 ml / kg body weight) was administered. In the case of the non-invasive blood pressure measurement method, rapid blood pressure fluctuations immediately after isoproterenol administration could not be detected, whereas in the invasive blood pressure measurement method using an indwelling needle, such blood pressure fluctuations were also detected. It was possible. As for the pulse rate, systolic blood pressure, diastolic blood pressure, and mean blood pressure, there are some differences between the measured values of the invasive blood pressure measurement method and the non-invasive blood pressure measurement method, but these are the measurement methods. This was due to the difference, and the fluctuation pattern of the measured values was the same in both cases. Further, the invasive blood pressure measurement using the above-mentioned indwelling needle was performed three times on the same individual at intervals of about one week. No modulation was observed in the physical condition of the animal or the state of the puncture site, and repeated measurement was possible.

[0014]

According to the present invention, it is possible to perform a reliable blood vessel treatment on an animal with little damage to the blood vessels.

[Brief description of the drawings]

FIG. 1 is a view showing the structure of an indwelling needle.

Claims (6)

[Claims] 1. A method for treating a blood vessel in an animal, comprising using an indwelling needle in a procedure for treating a blood vessel in an animal. 2. The method according to claim 1, wherein the animal is a small animal. 3. The animal according to claim 1, wherein the animal is a common marmoset.
The method for treating a blood vessel of an animal according to any one of claims 1 to 2. 4. The method according to claim 1, wherein the vascular treatment is blood collection. 5. The method according to claim 1, wherein the vascular treatment is intravenous administration.
The method for treating a blood vessel of an animal according to the above. 6. The method according to claim 1, wherein the vascular treatment is blood pressure measurement.