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IN2013MN00522A - - Google Patents

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Publication number
IN2013MN00522A
IN2013MN00522A IN522MUN2013A IN2013MN00522A IN 2013MN00522 A IN2013MN00522 A IN 2013MN00522A IN 522MUN2013 A IN522MUN2013 A IN 522MUN2013A IN 2013MN00522 A IN2013MN00522 A IN 2013MN00522A
Authority
IN
India
Prior art keywords
population
sequence
genomic sequence
surface bound
genomic
Prior art date
Application number
Inventor
Samuel Myllykangas
Jason Buenrostro
Hanlee P Ji
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of IN2013MN00522A publication Critical patent/IN2013MN00522A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Certain embodiments provide a method for capturing a genomic fragment. The method may comprise: obtaining a substrate comprising a first population of surface bound oligonucleotides and a second population of surface bound oligonucleotides; hybridizing a first member of the first population of surface bound oligonucleotides to a selection oligonucleotide comprising a region that hybridizes with the first member and a region that contains a genomic sequence; extending the first member of the first population of surface bound oligonucleotides to produce a support bound selection primer that comprises a sequence that is complementary to the genomic sequence; hybridizing the support bound selection primer to a nucleic acid fragment comprising the genomic sequence; extending the support bound selection primer to produce an extension product that contains a sequence that flanks the genomic sequence e.g. in a genome; and amplifying the extension product on the substrate.
IN522MUN2013 2010-09-24 2011-09-21 IN2013MN00522A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US38639010P 2010-09-24 2010-09-24
US201161485062P 2011-05-11 2011-05-11
PCT/US2011/052645 WO2012040387A1 (en) 2010-09-24 2011-09-21 Direct capture, amplification and sequencing of target dna using immobilized primers

Publications (1)

Publication Number Publication Date
IN2013MN00522A true IN2013MN00522A (en) 2015-05-29

Family

ID=45874160

Family Applications (1)

Application Number Title Priority Date Filing Date
IN522MUN2013 IN2013MN00522A (en) 2010-09-24 2011-09-21

Country Status (13)

Country Link
US (3) US9309556B2 (en)
EP (2) EP2619329B1 (en)
JP (1) JP5986572B2 (en)
KR (1) KR20130113447A (en)
CN (1) CN103228798B (en)
AU (1) AU2011305445B2 (en)
CA (1) CA2810931C (en)
IL (1) IL225109A (en)
IN (1) IN2013MN00522A (en)
MX (1) MX346956B (en)
NZ (1) NZ608313A (en)
RU (1) RU2565550C2 (en)
WO (1) WO2012040387A1 (en)

Families Citing this family (153)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
WO2010126614A2 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
CA2770389C (en) 2009-08-25 2015-12-29 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
JP5893607B2 (en) 2010-04-05 2016-03-23 プログノシス バイオサイエンシズ インコーポレイテッドＰｒｏｇｎｏｓｙｓ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． Spatial-encoded biological assay
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
JP5986572B2 (en) 2010-09-24 2016-09-06 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー Direct capture, amplification, and sequencing of target DNA using immobilized primers
GB2499340B (en) 2010-10-04 2015-10-28 Genapsys Inc Methods for sequencing nucleic acids
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP2768983A4 (en) 2011-10-17 2015-06-03 Good Start Genetics Inc Analysis methods
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
US10093975B2 (en) 2011-12-01 2018-10-09 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
EP3305918B1 (en) 2012-03-05 2020-06-03 President and Fellows of Harvard College Methods for epigenetic sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
EP2847353B1 (en) 2012-05-10 2022-01-19 The General Hospital Corporation Methods for determining a nucleotide sequence
GB2518078B (en) 2012-06-18 2015-04-29 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
WO2014015084A2 (en) * 2012-07-17 2014-01-23 Counsyl, Inc. System and methods for detecting genetic variation
US20140024541A1 (en) * 2012-07-17 2014-01-23 Counsyl, Inc. Methods and compositions for high-throughput sequencing
CN103571822B (en) * 2012-07-20 2016-03-30 中国科学院植物研究所 A kind of multipurpose DNA fragmentation enriching method analyzed for new-generation sequencing
MX367963B (en) * 2012-09-04 2019-09-11 Guardant Health Inc Systems and methods to detect rare mutations and copy number variation.
ES3019910T3 (en) 2012-10-17 2025-05-21 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
CN117845337A (en) 2012-12-10 2024-04-09 分析生物科学有限公司 Methods of targeted genomic analysis
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
GB2526736A (en) * 2013-02-21 2015-12-02 Toma Biosciences Inc Methods, compositions, and kits for nucleic acid analysis
US8778609B1 (en) 2013-03-14 2014-07-15 Good Start Genetics, Inc. Methods for analyzing nucleic acids
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
EP2971141B1 (en) 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
EP3005200A2 (en) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Methods and systems for storing sequence read data
CN118345148A (en) 2013-06-25 2024-07-16 普罗格诺西斯生物科学公司 Method and system for detecting spatial distribution of biological targets in a sample
US9898575B2 (en) 2013-08-21 2018-02-20 Seven Bridges Genomics Inc. Methods and systems for aligning sequences
US9116866B2 (en) 2013-08-21 2015-08-25 Seven Bridges Genomics Inc. Methods and systems for detecting sequence variants
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
WO2015058095A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for quantifying sequence alignment
WO2015058120A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for aligning sequences in the presence of repeating elements
CN105849279B (en) 2013-10-18 2020-02-18 七桥基因公司 Methods and systems for identifying disease-induced mutations
AU2014337089B2 (en) 2013-10-18 2019-08-08 Seven Bridges Genomics Inc. Methods and systems for genotyping genetic samples
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US9063914B2 (en) 2013-10-21 2015-06-23 Seven Bridges Genomics Inc. Systems and methods for transcriptome analysis
EP3068883B1 (en) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CN111100911B (en) * 2013-11-26 2023-06-02 杭州联川基因诊断技术有限公司 Method for amplifying target nucleic acid
EP3792921A1 (en) 2013-12-11 2021-03-17 Genapsys, Inc. Systems and methods for biological analysis and computation
JP2017500032A (en) * 2013-12-15 2017-01-05 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ Method for full-length amplification of double-stranded linear nucleic acid of unknown sequence
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
US9944924B2 (en) 2014-01-16 2018-04-17 Illumina, Inc. Polynucleotide modification on solid support
KR102001554B1 (en) * 2014-01-16 2019-07-18 일루미나, 인코포레이티드 Amplicon preparation and sequencing on solid supports
EP4219744A3 (en) 2014-01-27 2023-08-30 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
WO2015131107A1 (en) * 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
ES2876432T3 (en) * 2014-05-16 2021-11-12 Illumina Inc Nucleic acid synthesis techniques
US20150361422A1 (en) * 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
IL286474B2 (en) * 2014-06-23 2023-11-01 Massachusetts Gen Hospital Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
WO2016025818A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
JP2017536087A (en) 2014-09-24 2017-12-07 グッド スタート ジェネティクス， インコーポレイテッド Process control to increase the robustness of genetic assays
CA2964349C (en) 2014-10-14 2023-03-21 Seven Bridges Genomics Inc. Systems and methods for smart tools in sequence pipelines
EP3271480B8 (en) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Screening for structural variants
ES2955488T3 (en) 2015-04-10 2023-12-01 Spatial Transcriptomics Ab Multiplex analysis of biological specimens of spatially distinguished nucleic acids
EP3283629A4 (en) 2015-04-17 2018-08-29 President and Fellows of Harvard College Barcoding systems and methods for gene sequencing and other applications
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US10275567B2 (en) 2015-05-22 2019-04-30 Seven Bridges Genomics Inc. Systems and methods for haplotyping
US10718013B2 (en) * 2015-06-02 2020-07-21 BioFire Defense, LLC. et al. Sample to sequence
RU2604198C1 (en) * 2015-06-22 2016-12-10 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Method of producing heterogeneous set of single-chain dna fragments for multiplex genetic analysis
CN116064738A (en) * 2015-07-27 2023-05-05 亿明达股份有限公司 Spatial localization of nucleic acid sequence information
US10793895B2 (en) 2015-08-24 2020-10-06 Seven Bridges Genomics Inc. Systems and methods for epigenetic analysis
US10584380B2 (en) 2015-09-01 2020-03-10 Seven Bridges Genomics Inc. Systems and methods for mitochondrial analysis
US10724110B2 (en) 2015-09-01 2020-07-28 Seven Bridges Genomics Inc. Systems and methods for analyzing viral nucleic acids
CA3005985C (en) 2015-09-09 2025-04-22 Psomagen, Inc. Method and system for microbiome-derived diagnostics and therapeutics of infectious disease and other health conditions associated with antibiotic usage
AU2016325100B2 (en) * 2015-09-18 2022-01-20 Vanadis Diagnostics Probe set for analyzing a DNA sample and method for using the same
US11347704B2 (en) 2015-10-16 2022-05-31 Seven Bridges Genomics Inc. Biological graph or sequence serialization
KR101651817B1 (en) * 2015-10-28 2016-08-29 대한민국 Primer set for Preparation of NGS library and Method and Kit for making NGS library using the same
WO2017083562A1 (en) 2015-11-11 2017-05-18 Resolution Bioscience, Inc. High efficiency construction of dna libraries
US10364468B2 (en) 2016-01-13 2019-07-30 Seven Bridges Genomics Inc. Systems and methods for analyzing circulating tumor DNA
US10460829B2 (en) 2016-01-26 2019-10-29 Seven Bridges Genomics Inc. Systems and methods for encoding genetic variation for a population
US10262102B2 (en) 2016-02-24 2019-04-16 Seven Bridges Genomics Inc. Systems and methods for genotyping with graph reference
WO2017168331A1 (en) * 2016-03-28 2017-10-05 Boreal Genomics, Inc. Linked duplex fragment sequencing
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
US12416047B2 (en) 2016-05-06 2025-09-16 Myriad Women's Health, Inc. Noninvasive prenatal diagnostic methods
WO2017214557A1 (en) 2016-06-10 2017-12-14 Counsyl, Inc. Nucleic acid sequencing adapters and uses thereof
CN116397014A (en) 2016-07-20 2023-07-07 测序健康公司 Systems and methods for nucleic acid sequencing
WO2018027048A1 (en) * 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
RU2019108294A (en) 2016-08-25 2020-09-25 Резолюшн Байосайенс, Инк. METHODS FOR DETECTING CHANGES IN THE AMOUNT OF GENOMIC COPIES IN DNA SAMPLES
US11250931B2 (en) 2016-09-01 2022-02-15 Seven Bridges Genomics Inc. Systems and methods for detecting recombination
EP3512947B1 (en) 2016-09-15 2021-10-20 ArcherDX, LLC Methods of nucleic acid sample preparation
CN110023504B (en) 2016-09-15 2023-05-09 阿谢尔德克斯有限责任公司 Nucleic acid sample preparation method for analyzing cell-free DNA
EP3518974A4 (en) 2016-09-29 2020-05-27 Myriad Women's Health, Inc. Noninvasive prenatal screening using dynamic iterative depth optimization
JP7048609B2 (en) 2016-12-09 2022-04-05 ボリアル ジェノミクス， インコーポレイテッド Linked ligation
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
AU2018210188B2 (en) 2017-01-18 2023-11-09 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
WO2018144217A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
US10968447B2 (en) 2017-01-31 2021-04-06 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
US10787699B2 (en) * 2017-02-08 2020-09-29 Microsoft Technology Licensing, Llc Generating pluralities of primer and payload designs for retrieval of stored nucleotides
WO2018161019A1 (en) * 2017-03-03 2018-09-07 Counsyl, Inc. Methods for optimizing direct targeted sequencing
US11232850B2 (en) 2017-03-24 2022-01-25 Myriad Genetics, Inc. Copy number variant caller
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
EP3684951A4 (en) 2017-09-21 2021-06-16 Genapsys, Inc. Systems and methods for nucleic acid sequencing
CN111542532B (en) * 2017-10-04 2024-03-15 生捷科技控股公司 Method and system for synthesizing oligonucleotide by enzyme method
WO2019075197A1 (en) 2017-10-11 2019-04-18 The General Hospital Corporation Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2019157034A1 (en) * 2018-02-07 2019-08-15 Nugen Technologies, Inc. Library preparation
JP7460539B2 (en) 2018-04-17 2024-04-02 ザ ジェネラル ホスピタル コーポレイション IN VITRO sensitive assays for substrate selectivity and sites of binding, modification, and cleavage of nucleic acids
WO2019217452A1 (en) 2018-05-08 2019-11-14 Mgi Tech Co., Ltd. Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly
WO2020018824A1 (en) * 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
WO2020039261A1 (en) 2018-08-23 2020-02-27 Boreal Genomics, Inc. Linked target capture and ligation
CN113728114A (en) * 2018-10-09 2021-11-30 台湾地区“中央研究院” Digital polymerase chain reaction method for detecting nucleic acid in sample
CN113767177B (en) 2018-12-10 2025-01-14 10X基因组学有限公司 Generation of capture probes for spatial analysis
CN113348250B (en) * 2018-12-14 2024-12-10 深圳华大生命科学研究院 Nucleic acid synthesis device and purification device, use thereof, and nucleic acid synthesis method and purification method
CN112654714B (en) 2018-12-17 2024-10-11 伊卢米纳剑桥有限公司 Primer oligonucleotides for sequencing
EP3884071B1 (en) 2019-01-03 2025-03-26 Ncan Genomics, Inc. Linked target capture
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020237010A1 (en) * 2019-05-21 2020-11-26 Academia Sinica Method for amplifying and detecting ribonucleic acid (rna) fragments
US11639521B2 (en) * 2019-06-03 2023-05-02 The Chinese University Of Hong Kong Method for determining the copy number of a tandem repeat sequence
CN112342269B (en) * 2019-08-09 2023-12-05 深圳市真迈生物科技有限公司 A method for capturing nucleic acid molecules and its application
US11795495B1 (en) * 2019-10-02 2023-10-24 FOXO Labs Inc. Machine learned epigenetic status estimator
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
CN115103909A (en) * 2020-01-31 2022-09-23 艾维达生物医学公司 Systems and methods for targeted nucleic acid capture
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
FI3969613T3 (en) * 2020-02-26 2023-10-06 Illumina Inc Kits for genotyping
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
WO2021231263A2 (en) * 2020-05-12 2021-11-18 Singular Genomics Systems, Inc. Nucleic acid amplification methods
CN111676276A (en) * 2020-07-13 2020-09-18 湖北伯远合成生物科技有限公司 A method for rapid and accurate determination of gene editing mutation and its application
EP4153606A4 (en) 2020-07-13 2024-10-02 Singular Genomics Systems, Inc. Methods of sequencing complementary polynucleotides
EP4189109A4 (en) * 2020-07-31 2024-08-14 Arc Bio, LLC SYSTEMS, METHODS AND MEDIA FOR DETERMINING THE RELATIVE QUALITY OF OLIGONUCLEOTIDE PREPARATIONS
EP4211239A4 (en) * 2020-09-08 2024-09-11 Seqwell, Inc. SEQUENCING OLIGONUCLEOTIDES AND METHODS OF USE THEREOF
US12499970B2 (en) 2020-09-30 2025-12-16 Myriad Women's Health, Inc. Deep learning based variant calling using machine learning
RU2748380C1 (en) * 2020-11-10 2021-05-25 федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ) SET OF OLIGONUCLEOTIDE PRIMERS AND METHOD FOR GENOTYPING SINGLE NUCLEOTIDE POLYMORPHISM rs8065080 IN HUMAN TRPV1 GENE
CN112837749B (en) * 2021-02-01 2021-11-26 北京百奥纳芯生物科技有限公司 Optimization method of gene chip probe for cancer screening
WO2022232308A1 (en) * 2021-04-27 2022-11-03 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
KR20240031968A (en) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 Intensity extraction with interpolation and adaptation to base calling
KR20240036638A (en) * 2021-07-20 2024-03-20 프리놈 홀딩스, 인크. Compositions and methods for improved 5-hydroxymethylated cytosine resolution in nucleic acid sequencing
CN114621307B (en) * 2022-04-12 2024-12-13 中国科学院苏州生物医学工程技术研究所 A method for encoding oligonucleotide spatial coordinates and a microfluidic device thereof
WO2025072683A1 (en) * 2023-09-29 2025-04-03 Illumina, Inc. Solid supports and methods for strand invasion-based amplification

Family Cites Families (271)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3575220A (en) 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4739044A (en) 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US6090591A (en) 1987-07-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
AU4128089A (en) 1988-09-15 1990-03-22 Rorer International (Overseas) Inc. Monoclonal antibodies specific to human epidermal growth factor receptor and therapeutic methods employing same
US4997928A (en) 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US7223833B1 (en) 1991-05-24 2007-05-29 Isis Pharmaceuticals, Inc. Peptide nucleic acid conjugates
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
ATE198358T1 (en) 1992-04-27 2001-01-15 Dartmouth College DETECTION OF GENE SEQUENCES IN BIOLOGICAL LIQUIDS
ATE208658T1 (en) 1993-07-28 2001-11-15 Pe Corp Ny APPARATUS AND METHOD FOR NUCLEIC ACID DUPLICATION
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CH686982A5 (en) 1993-12-16 1996-08-15 Maurice Stroun Method for diagnosis of cancers.
US5539083A (en) 1994-02-23 1996-07-23 Isis Pharmaceuticals, Inc. Peptide nucleic acid combinatorial libraries and improved methods of synthesis
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5789206A (en) 1995-07-07 1998-08-04 Myriad Genetics, Inc. Method for ligating adaptors to nucleic acids which methods are useful for obtaining the ends of genes
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
NO954667D0 (en) 1995-11-17 1995-11-17 Dagfinn Oegreid Method for detecting Ki-ras mutations
EP0929694A4 (en) 1996-03-15 2002-05-02 Penn State Res Found Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
CA2249717A1 (en) 1996-03-18 1997-09-25 Richard K. Wilkosz Target nucleic acid sequence amplification
US6759217B2 (en) 1996-03-26 2004-07-06 Oncomedx, Inc. Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
SE9602829D0 (en) 1996-07-19 1996-07-19 Yngve Johnsson Procedure and apparatus for payment processing and means of payment
US5998140A (en) 1996-07-31 1999-12-07 The Scripps Research Institute Complex formation between dsDNA and oligomer of cyclic heterocycles
US6482795B1 (en) 1997-01-30 2002-11-19 Myriad Genetics, Inc. Tumor suppressor designated TS10q23.3
US6262242B1 (en) 1997-01-30 2001-07-17 Board Of Regents, The University Of Texas System Tumor suppressor designated TS10Q23.3
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JPH10341047A (en) 1997-06-06 1998-12-22 Sony Corp Magnetic tunnel element
CA2792122C (en) 1997-07-07 2015-09-08 Medical Research Council In vitro sorting method
US6008002A (en) 1997-09-29 1999-12-28 Bodey; Bela Immunomagnetic detection and isolation of cancer cells
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
IL137802A0 (en) 1998-02-12 2001-10-31 Immunivest Corp A method and kit for detecting rare cells in a mixed cell population
JPH11355695A (en) 1998-06-10 1999-12-24 Sony Corp Video signal processor
DE19833738A1 (en) 1998-07-27 2000-02-03 Michael Giesing Process for isolating cancer cells from cell-containing body fluids and kits for performing this process
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
AR021833A1 (en) 1998-09-30 2002-08-07 Applied Research Systems METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
WO2000040758A2 (en) * 1999-01-06 2000-07-13 Hyseq Inc. Enhanced sequencing by hybridization using pools of probes
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US9534254B1 (en) 1999-02-02 2017-01-03 Abbott Molecular Inc. Patient stratification for cancer therapy based on genomic DNA microarray analysis
US6395481B1 (en) 1999-02-16 2002-05-28 Arch Development Corp. Methods for detection of promoter polymorphism in a UGT gene promoter
US6410231B1 (en) 1999-02-26 2002-06-25 Incyte Genomics, Inc. SNP detection
US7324926B2 (en) 1999-04-09 2008-01-29 Whitehead Institute For Biomedical Research Methods for predicting chemosensitivity or chemoresistance
US20010034023A1 (en) 1999-04-26 2001-10-25 Stanton Vincent P. Gene sequence variations with utility in determining the treatment of disease, in genes relating to drug processing
US6492161B1 (en) 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
CA2383928A1 (en) 1999-09-01 2001-03-08 Whitehead Institute For Biomedical Research Chromosome-wide analysis of protein-dna interactions
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7205105B2 (en) 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
US20020012921A1 (en) 2000-01-21 2002-01-31 Stanton Vincent P. Identification of genetic components of drug response
JP2004514112A (en) 2000-01-26 2004-05-13 ヴェンタナ　メディカル　システムズ　インコーポレイテッド Assay development system for personalized medicine
WO2001064864A2 (en) 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
DE60142828D1 (en) 2000-03-01 2010-09-30 Epoch Biosciences Inc MODIFIED OLIGONUCLEOTIDES FOR THE DISTINCTION OF MALFUNCTIONS
US20020120409A1 (en) 2000-05-19 2002-08-29 Affymetrix, Inc. Methods for gene expression analysis
WO2001096382A2 (en) 2000-06-15 2001-12-20 Prokaria Ehf. Thermostable cellulase
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US7294503B2 (en) 2000-09-15 2007-11-13 California Institute Of Technology Microfabricated crossflow devices and methods
AU2001292802A1 (en) 2000-09-19 2002-04-02 Dana-Farber Cancer Institute Inc. Genetic markers for tumors
CA2426824A1 (en) 2000-10-24 2002-07-25 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic dna
US20050021240A1 (en) 2000-11-02 2005-01-27 Epigenomics Ag Systems, methods and computer program products for guiding selection of a therapeutic treatment regimen based on the methylation status of the DNA
US20030093819A1 (en) 2000-11-03 2003-05-15 D'andrea Alan D. Methods and compositions for the diagnosis of cancer susceptibilities and defective DNA repair mechanisms and treatment thereof
EP1207209A3 (en) 2000-11-09 2002-09-11 Agilent Technologies, Inc. (a Delaware corporation) Methods using arrays for detection of single nucleotide polymorphisms
EP1379686B1 (en) 2000-12-01 2012-09-05 Response Genetics, Inc. Method of determining epidermal growth factor receptor and her2-neu gene expression and correlation of levels thereof with survival rates
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US20020115073A1 (en) 2001-02-16 2002-08-22 Nickolas Papadopoulos Genome-based personalized medicine
JP3746766B2 (en) 2001-02-23 2006-02-15 独立行政法人科学技術振興機構 Emulsion production method and apparatus
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US20040110193A1 (en) 2001-07-31 2004-06-10 Gene Logic, Inc. Methods for classification of biological data
US20030165940A1 (en) 2001-12-06 2003-09-04 The Johns Hopkins University Disease detection by digital protein truncation assays
AU2002359645A1 (en) 2001-12-07 2003-07-09 University Of Massachusetts Targeted genetic risk-stratification using microarrays
US6949342B2 (en) 2001-12-21 2005-09-27 Whitehead Institute For Biomedical Research Prostate cancer diagnosis and outcome prediction by expression analysis
EP1456364B1 (en) 2001-12-21 2011-03-23 The Wellcome Trust Mutations in the b-raf gene
US7730063B2 (en) 2002-12-10 2010-06-01 Asset Trust, Inc. Personalized medicine service
EP1573316B1 (en) 2002-03-01 2009-08-26 Siemens Healthcare Diagnostics Inc. Assays for cancer patient monitoring based on levels of epidermal growth factor receptor (egfr) extracellular domain (ecd) analyte, alone or in combination with other analytes, in body fluid samples
AU2003213836A1 (en) 2002-03-11 2003-09-29 Epoch Biosciences, Inc. Negatively charged minor groove binders
US7427480B2 (en) 2002-03-26 2008-09-23 Perlegen Sciences, Inc. Life sciences business systems and methods
US7138226B2 (en) 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
AU2003249681A1 (en) 2002-05-31 2003-12-19 Diversa Corporation Multiplexed systems for nucleic acid sequencing
US9388459B2 (en) * 2002-06-17 2016-07-12 Affymetrix, Inc. Methods for genotyping
TWI266907B (en) 2002-07-24 2006-11-21 Nitto Denko Corp Polarizing device, optical thin film using the same, and image display device using the same
CA2499360A1 (en) 2002-09-19 2004-04-01 Applera Corporation Methods and compositions for detecting targets
DE60320119D1 (en) 2002-09-20 2008-05-15 Prokaria Ehf Thermostabile ligase aus thermus phage
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US8008003B2 (en) 2002-11-15 2011-08-30 Genomic Health, Inc. Gene expression profiling of EGFR positive cancer
JP4395133B2 (en) 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Single molecule amplification and detection of DNA
US8275554B2 (en) 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
US20040137539A1 (en) 2003-01-10 2004-07-15 Bradford Sherry A. Cancer comprehensive method for identifying cancer protein patterns and determination of cancer treatment strategies
ES2342665T3 (en) 2003-01-29 2010-07-12 454 Corporation SEQUENCING FROM TWO EXTREME.
US20090124514A1 (en) 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
WO2004083460A1 (en) 2003-03-20 2004-09-30 Csi Biotech Oy Amp ligation assay (ala)
EP1615993A4 (en) 2003-04-09 2012-01-04 Omicia Inc Methods of selection, reporting and analysis of genetic markers using broad based genetic profiling applications
US7056673B2 (en) 2003-04-25 2006-06-06 Janssen Pharmaceutica N.V. Preservation of RNA in a biological sample
AU2004248120B2 (en) 2003-05-28 2009-04-23 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
RU2392324C2 (en) * 2003-09-18 2010-06-20 Симфоген А/С Concerned sequences linkage method
CA2543786A1 (en) 2003-10-24 2005-05-06 Mmi Genomics, Inc. Methods and systems for inferring traits to manage non-beef livestock
US7381818B2 (en) 2003-10-28 2008-06-03 Epoch Biosciences, Inc. Fluorescent probes containing 5′-minor groove binder, fluorophore and quenching moieties and methods of use thereof
US7981618B2 (en) 2003-11-05 2011-07-19 Board Of Regents Of The University Of Texas System Diagnostic and therapeutic methods and compositions involving PTEN and breast cancer
US7439341B2 (en) 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
US20050181377A1 (en) 2004-02-13 2005-08-18 Markovic Svetomir N. Targeted cancer therapy
US20060195266A1 (en) 2005-02-25 2006-08-31 Yeatman Timothy J Methods for predicting cancer outcome and gene signatures for use therein
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US20060046258A1 (en) 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
KR20070048645A (en) 2004-03-01 2007-05-09 더 유니버시티 오브 시카고 Polymorphism in Epidermal Growth Factor Receptor Gene Promoter
WO2005094532A2 (en) 2004-03-24 2005-10-13 Applera Corporation Encoding and decoding reactions for determining target polynucleotides
ES2741546T3 (en) 2004-03-31 2020-02-11 Massachusetts Gen Hospital Method to determine the response of cancer to treatments directed at the epidermal growth factor receptor
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
EP2163650B1 (en) 2004-04-09 2015-08-05 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
WO2005108583A1 (en) 2004-05-06 2005-11-17 Prokaria Ehf. Thermostable polypeptide having polynucleotide kinase activity and/or phosphatase activity
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
EP2592155B2 (en) 2004-06-04 2019-09-11 Genentech, Inc. EGFR mutations
US7329495B2 (en) 2004-06-09 2008-02-12 Board Of Regents, The University Of Texas System Mutations in KIT confer imatinib resistance in gastrointestinal stromal tumors
WO2006012361A2 (en) 2004-07-01 2006-02-02 University Of Southern California Genetic markers for predicting disease and treatment outcome
WO2006017635A2 (en) 2004-08-11 2006-02-16 Albert Einstein College Of Medicine Of Yeshiva University Isolation, gene expression, and chemotherapeutic resistance of motile cancer cells
EP1805199B1 (en) 2004-10-18 2011-01-05 Brandeis University Methods for nucleic acid amplification
KR20070073917A (en) 2004-10-21 2007-07-10 뉴 잉글랜드 바이오랩스, 인크 Nucleic Acid Recovery for Improved Amplification
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US20060278241A1 (en) 2004-12-14 2006-12-14 Gualberto Ruano Physiogenomic method for predicting clinical outcomes of treatments in patients
US20060184489A1 (en) 2004-12-17 2006-08-17 General Electric Company Genetic knowledgebase creation for personalized analysis of medical conditions
EP1851335B1 (en) 2005-01-21 2014-11-26 Gene Express, Inc. Compositions and methods of use of standardized mixtures
US7442507B2 (en) 2005-01-24 2008-10-28 New York University School Of Medicine Methods for detecting circulating mutant BRAF DNA
WO2006081558A2 (en) 2005-01-28 2006-08-03 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US7635570B2 (en) 2005-04-01 2009-12-22 Salvatore Siena Epidermal growth factor receptor gene copy number
ATE406463T1 (en) 2005-04-06 2008-09-15 Maurice Stroun METHOD FOR CANCER DIAGNOSIS USING DETECTION OF DNA AND RNA IN THE CIRCULATION
CN101155932A (en) 2005-04-14 2008-04-02 默克专利有限公司 anti-EGFR antibody treatment based on increased EGFR gene copy number in tumor tissue
JP4547301B2 (en) 2005-05-13 2010-09-22 株式会社日立ハイテクノロジーズ Liquid transport device and analysis system
US20070020657A1 (en) 2005-05-20 2007-01-25 Grebe Stefan K Methods for detecting circulating tumor cells
DE602006016106D1 (en) 2005-05-31 2010-09-23 Dako Denmark As COMPOSITIONS AND METHOD FOR PREDICTING THE OUTPUT OF TREATMENT
DK1913157T4 (en) 2005-06-28 2017-01-23 Genentech Inc EGFR and KRAS mutations for predicting patient response to EGFR inhibitor therapy.
GB0514909D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
CN101263227A (en) 2005-09-16 2008-09-10 454生命科学公司 cDNA library preparation
EP1946114A4 (en) 2005-09-21 2010-05-26 Ccc Diagnostics Llc EXHAUSTIVE DIAGNOSTIC TEST PROCEDURES FOR PERSONALIZED ANTICANCER CHEMOTHERAPIES
US20070172844A1 (en) 2005-09-28 2007-07-26 University Of South Florida Individualized cancer treatments
ATE536420T1 (en) 2005-10-24 2011-12-15 Univ Johns Hopkins IMPROVED METHODS FOR BEAMING
CN101365803B (en) 2005-12-22 2013-03-20 关键基因股份有限公司 Improved strategies for transcript profiling using high throughput sequencing technologies
US20100304446A1 (en) 2006-02-07 2010-12-02 Stokes Bio Limited Devices, systems, and methods for amplifying nucleic acids
ATE523244T1 (en) 2006-02-07 2011-09-15 Stokes Bio Ltd LIQUID BRIDGE SYSTEM AND METHOD
EP1991357B1 (en) 2006-02-07 2016-09-14 Stokes Bio Limited A microfluidic analysis system
EP2290361A1 (en) 2006-02-16 2011-03-02 Ventana Medical Systems, Inc. Methods for selecting a colorectal carcinoma treatment
DK1991698T3 (en) 2006-03-01 2014-03-10 Keygene Nv "High-throughput" -sekvensbaseret detection of SNPs using ligeringsassays
US8086005B2 (en) 2006-03-08 2011-12-27 Olympus Medical Systems Corp. Medical image processing apparatus and medical image processing method
WO2007106432A2 (en) 2006-03-10 2007-09-20 George Mason Intellectual Properties, Inc. Egf receptor phosphorylation status for disease treatment
US7908091B2 (en) 2006-03-17 2011-03-15 Prometheus Laboratories Inc. Methods of predicting and monitoring tyrosine kinase inhibitor therapy
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
CA2647102A1 (en) 2006-03-27 2007-11-22 Globeimmune, Inc. Ras mutation and compositions and methods related thereto
TWI395754B (en) 2006-04-24 2013-05-11 Amgen Inc Humanized c-kit antibody
US8380539B2 (en) 2006-05-09 2013-02-19 University Of Louisville Research Foundation, Inc. Personalized medicine management software
EP2021113A2 (en) 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
IL282783B2 (en) 2006-05-18 2023-09-01 Caris Mpi Inc System and method for determining individualized medical intervention for a disease state
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
WO2008111990A1 (en) 2006-06-14 2008-09-18 Cellpoint Diagnostics, Inc. Rare cell analysis using sample splitting and dna tags
WO2008015396A2 (en) 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
US20080065411A1 (en) 2006-09-08 2008-03-13 Diaceutics Method and system for developing a personalized medicine business plan
EP2074226A2 (en) 2006-09-19 2009-07-01 Novartis AG Biomarkers of target modulation, efficacy, diagnosis and/or prognosis for raf inhibitors
WO2008038259A1 (en) 2006-09-28 2008-04-03 Stokes Bio Limited A qpcr analysis apparatus
US8568979B2 (en) 2006-10-10 2013-10-29 Illumina, Inc. Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization
CA2667737A1 (en) 2006-10-27 2008-05-02 Julius Gudmundsson Cancer susceptibility variants on chr8q24.21
KR20090095571A (en) 2006-11-01 2009-09-09 조지 매이슨 인털렉춸 프로퍼티즈, 인코퍼레이티드 Method for detecting and controlling cancer
US20100196889A1 (en) 2006-11-13 2010-08-05 Bankaitis-Davis Danute M Gene Expression Profiling for Identification, Monitoring and Treatment of Colorectal Cancer
US20080131887A1 (en) 2006-11-30 2008-06-05 Stephan Dietrich A Genetic Analysis Systems and Methods
WO2008115300A1 (en) 2006-12-01 2008-09-25 Apocell, Inc. C-kit phosphorylation in cancer
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
JP5622392B2 (en) 2006-12-14 2014-11-12 ライフ テクノロジーズ コーポレーション Method and apparatus for analyte measurement using large-scale FET arrays
US20080177608A1 (en) 2007-01-19 2008-07-24 Diaceutics Business method for enabling personalized medicine
JP2010528585A (en) 2007-02-26 2010-08-26 ジョン ウェイン キャンサー インスティテュート Usefulness of B-RAF DNA mutations in the diagnosis and treatment of cancer
CA2679548C (en) 2007-03-02 2015-01-27 Amgen Inc. Methods and compositions for treating tumor diseases
ES2458626T5 (en) 2007-03-13 2022-04-29 Amgen Inc K-ras mutations and anti-EGFr antibody therapy
SI2412828T1 (en) 2007-03-13 2013-10-30 Amgen Inc. K-ras and B-raf mutations and anti-EGFr antibody therapy
US20090258795A1 (en) 2007-03-15 2009-10-15 Genomic Health, Inc. Gene expression markers for prediction of patient response to chemotherapy
US20080242622A1 (en) 2007-03-19 2008-10-02 Cold Spring Harbor Laboratory Identification of genetic alterations that modulate drug sensitivity in cancer treatments
CA2683804A1 (en) 2007-04-13 2008-10-23 Dana Farber Cancer Institute, Inc. Receptor tyrosine kinase profiling
US20080255243A1 (en) 2007-04-13 2008-10-16 Petricoin Emanuel F Stat3 as a theranostic indicator
WO2008147879A1 (en) 2007-05-22 2008-12-04 Ryan Golhar Automated method and device for dna isolation, sequence determination, and identification
WO2008148072A2 (en) 2007-05-24 2008-12-04 The Brigham And Women's Hospital, Inc. Disease-associated genetic variations and methods for obtaining and using same
ES2559313T3 (en) 2007-06-19 2016-02-11 Stratos Genomics Inc. High performance nucleic acid sequencing by expansion
GB0712882D0 (en) * 2007-07-03 2007-08-15 Leicester University Of Nucleic acid amplification
NZ582618A (en) 2007-07-13 2012-07-27 Prometheus Lab Inc Drug selection for lung cancer therapy using antibody-based arrays
JP5519500B2 (en) 2007-07-23 2014-06-11 ザ チャイニーズ ユニバーシティー オブ ホンコン Determination of nucleic acid sequence imbalance
WO2009021019A1 (en) 2007-08-06 2009-02-12 Health Research Inc. Methods for analysis of pdef and survivin as interconnected cancer biomarkers and targets for personalized medicine
US20090062138A1 (en) 2007-08-31 2009-03-05 Curry Bo U Array-based method for performing SNP analysis
US20110212991A1 (en) 2007-09-11 2011-09-01 Roche Molecular Systems, Inc. Diagnostic Test for Susceptibility to B-RAF Kinase Inhibitors
US20100173294A1 (en) 2007-09-11 2010-07-08 Roche Molecular Systems, Inc. Diagnostic test for susceptibility to b-raf kinase inhibitors
US9388457B2 (en) * 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
EP2201094A4 (en) * 2007-09-17 2010-10-06 Twof Inc Hydrogel labeled primer extension method for microarrays
CA2701726A1 (en) 2007-10-04 2009-04-09 Halcyon Molecular Sequencing nucleic acid polymers with electron microscopy
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
US20090264298A1 (en) 2007-11-06 2009-10-22 Ambergen, Inc. Methods for enriching subpopulations
EP2063132A1 (en) * 2007-11-24 2009-05-27 Festo AG & Co. KG Linear drive device
US8530197B2 (en) 2008-01-09 2013-09-10 Applied Biosystems, Llc Method of making a paired tag library for nucleic acid sequencing
EP2245191A1 (en) 2008-01-17 2010-11-03 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
US11051733B2 (en) 2008-01-18 2021-07-06 Wake Forest University Health Sciences Isolating and purifying cells for therapy
CN102084000B (en) 2008-02-01 2016-03-16 总医院有限公司 Use of microbubbles in the diagnosis, prognosis and treatment of medical diseases and conditions
EP2245198A1 (en) 2008-02-04 2010-11-03 Massachusetts Institute of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
WO2009102957A2 (en) 2008-02-14 2009-08-20 The Johns Hopkins University Methods to connect gene set expression profiles to drug sensitivity
CA2714521A1 (en) 2008-02-14 2009-08-20 Decode Genetics Ehf Susceptibility variants for lung cancer
ES2398618T3 (en) 2008-02-25 2013-03-20 Nestec S.A. Drug selection for breast cancer therapy using antibody matrices
US20090226975A1 (en) * 2008-03-10 2009-09-10 Illumina, Inc. Constant cluster seeding
WO2009114836A1 (en) 2008-03-14 2009-09-17 Genomic Health, Inc. Gene expression markers for prediction of patient response to chemotherapy
US20090307180A1 (en) 2008-03-19 2009-12-10 Brandon Colby Genetic analysis
US7842248B2 (en) 2008-06-20 2010-11-30 Silverbrook Research Pty Ltd Microfluidic system comprising microfluidic pump, mixer or valve
EP2291533B2 (en) 2008-07-02 2020-09-30 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US20100041048A1 (en) 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
JP2010041985A (en) 2008-08-18 2010-02-25 Hitachi Plant Technologies Ltd Method for amplifying nucleic acid sequence, method for detecting nucleic acid sequence and substrate for nucleic acid amplification and detection
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
GB2477053B (en) 2008-09-23 2013-11-13 Quantalife Inc Droplet-based assay system
EP2347009A4 (en) 2008-10-14 2012-05-30 Caris Mpi Inc GENETIC AND PROTEIN TARGETS EXPRESSED BY GENES REPRESENTING BIOMARKER PROFILES AND SIGNATURE SETS BY TYPE OF TUMORS
US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8349563B2 (en) 2008-11-18 2013-01-08 Life Technologies Corporation Sequence amplification with target primers
EP2366162A1 (en) 2008-11-18 2011-09-21 Collabrx, Inc. Individualized cancer treatment
EP2376659B1 (en) 2008-12-17 2015-12-02 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
US8790873B2 (en) 2009-01-16 2014-07-29 Affymetrix, Inc. DNA ligation on RNA template
JP2012517813A (en) 2009-02-16 2012-08-09 エピセンター テクノロジーズ コーポレイション Template-independent ligation of single-stranded DNA
US20100286143A1 (en) 2009-04-24 2010-11-11 Dora Dias-Santagata Methods and materials for genetic analysis of tumors
DK2511843T3 (en) 2009-04-29 2017-03-27 Complete Genomics Inc METHOD AND SYSTEM FOR DETERMINING VARIATIONS IN A SAMPLE POLYNUCLEOTIDE SEQUENCE IN TERMS OF A REFERENCE POLYNUCLEOTIDE SEQUENCE
WO2012083189A2 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
CA2770389C (en) 2009-08-25 2015-12-29 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
EP2494069B1 (en) * 2009-10-30 2013-10-02 Roche Diagniostics GmbH Method for detecting balanced chromosomal aberrations in a genome
WO2011060014A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
WO2011066476A1 (en) 2009-11-25 2011-06-03 Quantalife, Inc. Methods and compositions for detecting genetic material
US20110157322A1 (en) 2009-12-31 2011-06-30 Broadcom Corporation Controlling a pixel array to support an adaptable light manipulator
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
AU2011221243B2 (en) 2010-02-25 2016-06-02 Advanced Liquid Logic, Inc. Method of making nucleic acid libraries
WO2011123749A1 (en) 2010-04-01 2011-10-06 New England Biolabs, Inc. Compositions and methods for adenylating oligonucleotides
US9255291B2 (en) 2010-05-06 2016-02-09 Bioo Scientific Corporation Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
US20110299645A1 (en) 2010-06-07 2011-12-08 Korea Hydro & Nuclear Power Co., Ltd. Breeding Nuclear Fuel Mixture Using Metallic Thorium
WO2012003374A2 (en) 2010-07-02 2012-01-05 The Board Of Trustees Of The Leland Stanford Junior University Targeted sequencing library preparation by genomic dna circularization
EP2598660B1 (en) 2010-07-26 2017-03-15 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
JP5986572B2 (en) 2010-09-24 2016-09-06 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー Direct capture, amplification, and sequencing of target DNA using immobilized primers
WO2012054933A2 (en) 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods
KR20210131432A (en) 2010-12-30 2021-11-02 파운데이션 메디신 인코포레이티드 Optimization of multigene analysis of tumor samples
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
CN103748236B (en) 2011-04-15 2018-12-25 约翰·霍普金斯大学 Safe sequencing system
EP3351631B1 (en) 2011-09-07 2020-11-04 X-Chem, Inc. Methods for tagging dna-encoded libraries
PL2768985T3 (en) 2011-10-21 2019-10-31 Chronix Biomedical Colorectal cancer associated circulating nucleic acid biomarkers
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013113012A2 (en) 2012-01-27 2013-08-01 The Board Of Trustees Of The Leland Stanford Junior University Methods for profiling and quantitating cell-free rna
WO2013119690A1 (en) 2012-02-06 2013-08-15 Wisconsin Alumni Research Foundation Nucleic acid ligation method
WO2013173472A1 (en) 2012-05-15 2013-11-21 Predictive Biosciences, Inc. Methods of assessing chromosomal instabilities
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9816120B2 (en) 2013-01-09 2017-11-14 The Penn State Research Foundation Low sequence bias single-stranded DNA ligation
US20140287937A1 (en) 2013-02-21 2014-09-25 Toma Biosciences, Inc. Methods for assessing cancer
GB2526736A (en) 2013-02-21 2015-12-02 Toma Biosciences Inc Methods, compositions, and kits for nucleic acid analysis
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
ES2707744T3 (en) 2013-12-11 2019-04-04 Accuragen Holdings Ltd Methods to detect rare sequence variants
WO2017139492A1 (en) 2016-02-09 2017-08-17 Toma Biosciences, Inc. Systems and methods for analyzing nucelic acids

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