IL95419A - Bisphosphonates, a process for preparing them and pharmaceutical compositions containing them - Google Patents

Description

ΡΠΊ *>■» j n mnpn 'Tu m ,ρπυπ^ -|Οπη , D-1 O J I DPI OP-1 a Bisphosphonates, a Process for Preparing them and Pharmaceutical Compositions Containing them The present invention relates to novel bisphosphonates, processes for preparing them and pharmaceutical compositions containing them.

According to the present invention the compounds and the pharmaceutical compositions are suitable and useful for the treatment of irregularities in calcium metabolism.

More specifically the present invention relates to m and I are independently 1 or 2 R^j^ represents a hydrogen, or lower alkyl group, or an alkali metal cation; R2 represents a hydrogen, a lower alkyl group or an alkali metal cation; Y represents =0 or =N-0H, or -OH; X represents ~(CH2^n~ or a brancned alkylene group or a branched or straight alkenylene or alkynylene chain optionally substituted by one or more oxygen or nitrogen atoms; wherein n is an integer from 3 to 24 with the provision that when £=m=l, Y is =0 and R.. and R_ denote a lower alkyl group, n represents an integer from 9 to 20; and with the provision that when (=m=2 and Y is -OH and and R2 are methyl groups, represents an integer from 9-24; and with further provision that when (=m=2 Y is an-OH group and R1=R2=hydrogen, n represents 3,5, and an integer from 7-24. or X is -(CH2) - (OCH2CH2) - 0 - (CH2) - P q ' wherein p and p' are independently integers from 1 to 5 q is an integer from 1 to 6 or X is -(CH2) - 0 - (CH2) - O - (CH2) - t S t ' wherein t and t · are independently integers from 1 to 6 s is an integer from 2 to 12 or X is - B - A - B wherein B represents a branched group or straight alkylene, an alkenylene or alkynylene chain optionally substituted by one or more oxygen or nitrogen atoms. 954 jg 2 A represents an aromatic group selected from phenylene, naphthalenediyl, thiophenediyl or furandiyl.

There are several pathological conditions that involve irregularities in calcium metabolism. Such are some bone related diseases as Paget 's disease, osteoporosis as well as osteolysis in bone metastases.

Bone metastases present a major problem in many frequently occurring malignancies. Hypercalcemia, resulting from bone resorption, is a common and very important complication of malignancy, causing most distressful symptoms> such as severe pain, spontaneous fractures, and may lead to a metabolic coma and death. Moreover, neoplastic cell-induced osteolysis may determine the localization and growth enhancement of the tumor. (G.R. Mundy, Bone, 8, supp. 1, S9-5 16 (1987); Calcium in Biological Systems, R.P. Rubin, G.B. Weiss, and J.W. Putney, Jr. eds. Plenum Press, N.Y. 1985). Ectopic calcification is a seemingly opposite type of pathological condition, characterized by the deposition of calcium phosphate in a number of clinically important diseases as for example atherosclerosis, kidney and renal calculus, arthritis, and bioprosthetic heart valve calcification, and implanted biomaterial calcification such as bioprostetic and prostetic heart valves, vascular grafts, LVAD (left ventricular assist devices), contact lenses and a total artifical heart.

Bisphosphonates are a relatively new class of drugs that have been developed for use in various metabolic diseases of bone, the target being excessive bone resorption and inappropriate calcification and ossification. (M.D. Francis and R.R. artodam, in "The Role of Phosphonates in Living Systems" R.L. Hilderbrand, ed. CRC Press, Boca Raton, Fla, 1983, pp. 55-96; H. Fleisch, Bone, 1987, 8, Supp. 1, S23-S28 ) . Recently there have been reports of encouraging clinical trials utilizing bisphosphonates to treat hypercalcemia in patients with breast cancer, myeloma, and bronchial carcinoma related osteolytic metastases, in addition to the established usage of bisphosphonates in Paget 's disease and for diagnostic purposes in bone mapping. However, bisphosphonate therapy is frequently accompanied by severe side effects. Bisphosphonates have been also found highly potent both in inhibiting bioprosthetic heart valve calcification, and in experimental arteriosclerosis, however, this was accompanied by severe adverse effects on bone development and overall somatic growth.

The currently used bisphosphonates all belong to the geminal type, in which the two phosphoryl groups are bound to the same carbon ("P-C-P" ), and therefore may be viewed as pyrophosphate analogs in which the oxygen between the two phosphorus atoms is replaced by a carbon.

In contrast, monophosphonates, vicinal bisphosphonates (P-C-C-P) and compounds in which the distance between the phosphoryl group is longer (P-(C)n~P, n>2) are reported to be less active or inactive at. all.

From the results obtained in various clinical studies using conventional bisphosphonates it appears that there is a need for compounds which have greater margin between the bone resorption inhibiting effect and that inhibiting mineralization, without an increase in toxicity.

According to the present invention it was found that introduction of modifications into long chain bisphosphonates of type P-(C) -P increases the cation binding ability of these compounds, and inhibits ectopic calcification. The advantage in this "type of compound in interacting with calcium phosphate crystals is assumed to derive from the presence of an additional independent anchor site(s) in the molecule as compared with known bisphosphonates. An additional advantage of this novel class of compounds is an effect of long duration and the enhanced ability to interact with the cell membrane.

Patent no. 3,012,054 from March 18, 1960 and a paper by M. Kanaan and R. Burgada, Phosphorus and Sulfur, 1988, 37, 217-229, describe the preparation of "tetraalkyl esters of diphosphonates" having the structure: 0 0 0 0 (R0)2P II - CII - (CH2)n- CII - PII (0R)2 R - alkyl radical containg 1-4 cabons and n = 2 to 8, inclusive.

It should be emphasized that the patent mentioned deals only with tetraesters. It is well known that such dialkyl acylphosphonates, as mentioned, exhibit exteme instability toward water, and they hydrolyze to the corresponding carboxylic acids both in acidic and alkline conditions.

Consequently, hydrolysis of the tetraalkyl esters described in the patent and the paper cited above would lead to dicarboxylic acids HOOC-(CH2)n-COOH. Therefore, the syntheses of dealkylated derivatives such as represented by the formulas below: 0 0 0 0 0 0 - 0 0 R0-P-C-(CH_ ) -C-P-OR and H0-P-C-(CH„ ) -C-P-OH M+ -0 I 2 n 0I- M+ M+ -0I 2 n 0I- M+ require special nonhydrolytic methods, and by no means are the dealkylated compounds obvious derivatives of the teraesters.

Neither esters nor acids of bisphosphonates in which the two ketophosphonic groups such as aromatic rings etc., have been reported.

The present invention relates to novel bisphosphonates of the formula (I) R R, wherein m and I are independently 1 or 2 R^ represents a hydrogen, or lower alkyl group, or an alkali metal cation; R2 represents a hydrogen, a lower alkyl group or an alkali metal cation; Y represents =0 or =N-0H, or -OH; X represents -(CH-) - or a branched alkylene group or a branched or straight alkenylene or alkynylene chain optionally substituted by one or more oxygen or nitrogen atoms; wherein n is an integer from 3 to 24 with the provision that when abd 2 denote a lower alkyl group, n represents an integer from 9 to 20; . and with the provision that when and are methyl groups, n represents an integer from 9-24; and with further provision that when Y is an-OH group and n represents 3,5, and an integer from or X is -(CH2) - (OCH2CH2) - 0 - (CH2) - p q P' wherein p and p' are independently integers from 1 to 5 q is an integer from 1 to 6 or X is -(CH ) - 0 - (CH ) - 0 - (CH ) - t s t» wherein t and t ' are independently integers from 1 to 6 s is an integer from 2 to 12 or X is - B - A - B wherein B represents branched or straight alkylene, alkenylene or alkynylene chain optionally substituted by one or more oxygen or nitrogen atoms, A represents an aromatic group such as phenylene, naphthalenediyl, thiophenediyl or furandiyl.

Preferably is hydrogen or a methyl. group 1*2 is a methyl group, or sodium or lithium cation Y is =0 or =N0H B is -CH^- group A is phenylene optionally substituted.

The present invention further relates to a process for obtaining the abovementioned compounds according to formula (I) and pharmaceutical compositions containing them.

The process for the preparation of tetraalkyl α,α'-diketobisphosphonates comprises of adding trimethyl phosphite to the corresponding dicarboxylic acid dihalide.

The process for obtaining dialkyl αα'-diketobisphosphonates disalts comprises of adding tetraalkyl diketobisphosphonates dissolved in acetone or acetonitrile to a solution of sodium iodide or lithium bromide in acetonitrile .

The process for the preparation of hydrogen α,α'-diketobisphosphonate disalts (sodium) comprises of dissolving tetraalkyl diketobisphosphonate in dry nonhydroxylic solvent and addition of bromotrimethylsilane.

The process for the synthesis of dialkyl α,α'-bishyroxyiminobisphosphonate disalts comprises of suspending dimethyl dilithium a, α' -bishydroxyiminobisphosphonate, in absolute ethanol, and in a separate flask dissolving sodium in absolute ethanol . The resulting solution is added slowly to hydroxylamine hydrochloride in methanol .

The process for the synthesis of octa-alkyl aw' dihydroxy a ww tetrakisphosphonates is by adding aw diacyldihalide to a mixture of trialkyl phosphite and dialkyl phosphite .

The process for the synthesis of aw dihydroxy aa ww 10 0 tetrakisphosphonic acid consists of adding hydrochloric acid to the octaalkyl esters.

The present invention also relates to pharmaceutical compositions which comprise a compound according to the invention as active ingredient and suitable carriers optionally suitable for controlled release delivery systems and/or other additives.

The drug delivery systems may include any conventional suitable carrier or controlled release system (sustained release, delayed action preparations), based on a polymeric vehicle (e.g. silicon, polyurethane, or any other biocompatible polymer), or based on degradable systems (e.g. chitosan, collagen, or any other degradable/biodegradable carrier) .

Chitosan is soluble only in acidic H, preferably by acetic acid. Drug delivery systems based on chitosan as a carrier can be prepared in a conventional and in an innovative way. In the first method the drug is dissolved with the polymer in acidic pH (preferably, 1 to 10% w/w solids with acetic acid) and the solvent is evaporated or extracted by a non-solvent. By this method sustained release drug delivery systems in the form of film (matrix), micromatrics, microcapsules or microspheres could be prepared.

An innovative methods of preparing chitosan-based drug delivery system is based on the alkalinity of chitosan (amino functional groups) and the acidity of phosphonates (as free acid obtained from the sodium salt by a catonic exchange resin). The drug in its acid form is reacted with chitosan yielding a soluble chitosan-phosphonate salt without the requirement for another exterior acid, followed by water evaporation/extraction as above. By this method the controlled release of the drug is governed not only by the matrix but also by the dissociation of the carrier-drug salt. Additional advantage is the possible targeting of the drug by chitosan. The chitosan-phosphonate salt could be embedded in chitosan for further delay of drug release.

The treatment with the controlled release delivery system is utilized by subdermal implantation (as was done in Fig. 1) or by site specific implantation, with the aim being optimization of therapy, using lower dosage, minimizing systemic side effects, and effective prolonged treatment with better patient compliance.

The novel bisphosphonates, according to the present invention, prevent calcium precipitation from metastable calcium and phosphate solution. Profound inhibition of rat, subdermal bioprosthetic heart valve tissue calcification was achieved, by coimplantion of Alzet osmotic pumps releasing the drug, and tissue cusps. Therapy was achieved without side effects, as exhibited by the normal somatic growth.

These in-vivo results are summarized in Fig. 1.

The novel bisphosphonates, according to the present invention are useful in the treatment of the following diseases: Osteoporosis (including disuse and postmenopausal osteoporosis), Hypercalcemia of Malignancy, (Direct) anticancer effect, Heterotopic Ossification (Hip Arthroplasty, Spinal cord injury, Myositis ossificans), Paget 's disease, Hyperphosphatemia (e.g. Diabetes).

It can be seen that said compounds are useful not only for direct treatment of various diseases but also for treatment of the symptoms of the diseases (e.g.

Hyperphosphatemia or Hypercalcemia).

The compounds according to the present invention are also useful as diagnostics (e.g. Nuclear Medicine).

The compounds -according to the present invention may possess also industrial applications which are listed below (R.L. Hilderbrand, The Role of Phosphonates in Living Systems, Chapter 7, page 172, CRC Press); Adhesives; Agents for extraction, concentration, and purification of uranium, thorium, and plutonium; Antioxidants; Antistatic agents; Blowing agents; Catalysts; Corrosion inhibitors; Coupling agents; Crystallization inhibitors; Dentifrice compositions; Deodorants; Detergent additives; Detergents for cleaning metal surfaces; Dye modifiers; Flame retardant polymers; Flame retardants for textiles; Fire retardants for synthetic fibers; Flotation agents; Fuel additives; Gelling agents; Hardening oil composites; Heat and light stablizers; Hydraulic fluid additives; Ion exchange resins; Lubricants; Photography; Plasticizers; Polyester, polyethylene, and polycarbonate discoloration inhibitors; Polyurethan additives; Rayon additives; Resin and plastic additives; Scale inhibitors; Settling retardants; Sequestering agents; Solvent extraction; Suspending agents; Synthetic fiber preparation; Viscosity modifiers; Wood fireproofing agents.

The invention is further illustrated by means of the following non-limiting examples.

Examples General method for the synthesis of tetraalkyl α,α'-diketobisphosphonates . Trimethyl phosphite (0.4 mole) was added dropwise to the dicarboxylic acid dichloride (0.2 mole) o at 5 C. After the addition was completed, the reaction mixture was allowed, to stir for 1 hr at ambient temperature. Tetramethyl adipoylbisphosphonate was obtained in a yield of 90%. IR (neat) 1697s, 1260s, 1030s cm"1. NMR; (CDC13) ½: δ 3.87 (12H, J=10.64 Hz), 2.85 (4H, m), 1.65 (4H, m). Tetramethyl suberoylbisphosphonate was obtained in a yield of 90%. IR spctrum (neat) 1696s, 1265s, 1034s cm-1. NMR (CDClg); ½: S" 3.87 (12H, d, J=10 Hz), 2.82 4H, t, J=7.2 Hz), 1.63 (4H, m), 1.32 (4H, m), 31P: 6 =0.88 (sept).

These compounds decomposed upon attempted distillation, but they were sufficiently pure to be used for the next step in the synthesis without further purification.

Synthesis of Me203P-CO-(CH2 )10-CO-P03Me2 Tetramethyl dodecanedioyldiphosphonate . 8.03 g (0.03 mol ) of dodecanedicyl dichloride was added, drop by drop, with stirring to a solution of 7.82 g (0.0636 mol) trimethyl phosphite in dry toluene at -10 C under a nitrogen atmoshphere . The reaction mixture was stirred at room temperature for about 24 h. the toluene and the excess trimethyl phosphite were evaporated at reduced pressuire to yield an oily product. In the 31P nmr spectrum the product showed a septet at the chemical shift of 6 -4.4 ppm.

General method for the synthesis of dialkyl α,α'-diketobisphosphonate disalts. Tetraalkyl diketobisphosphonate (0.5 mole) was dissolved in 50 ml dry acetone or acetonitrile, and the solution was added to a solution of sodium iodide (1.1 mole) in dry acetone (30 ml) or lithium bromide in acetonitrile. The reaction mixture was stirred overnight "at room temperature. The precipitate was filtered, washed with dry acetone or acetonitrile and dried. The yields are > 85%.

Dimethyl dilithium adipoylbisphosphonates, yield was 95%, m.p. > 250°C, IR (nujol): 1660s, 1210s, 1110s, 1020s cm"1. NMR (D20) 1H: 6 3.6 (6H, d, J=10.67 Hz), 2.85 (4H, m), 1.6 (4H, m).

Dimethyl dilithium suberoylbisphosphonate, yield 100%, m.p.>250°C, IR (nujol): 1670s, 1216s, 1110s, 1040s cm-1. MR (D20) ½ 6 3.65 (6H, d, J=10.56 Hz), 2.87 (4H, t, J=7.2 Hz), 1.64 (4H, m) 1.36 (4H, m).

General method for the synthesis of dihydrogen α,α'-diketobisphosphonate disalts (sodium). 0.01 mole of tetramethyl diketobisphosphonate was dissolved in dry acetonitrile (20 ml). 0.066 mole (9 ml) of bromo rime hy1silane was added slowly and the reaction mixture was stirred at ambient temperature for three hours.

The acetonitrile was evaporated in vacuum (keeping the o temperature below 30 C). A solution of sodium hydroxide (0.02 Mole) in methanol (25 ml) was added to the residue and the reaction mixture was stirred overnight at the ambient temperature. The white precipitate was filtered, washed with methanol (15 ml) and dried.

Disodium dihydrogen adipoylbisphosphonate, yield 90%, m.p.>250°C, IR KBr 1675s, 1191s, 1055s cm'1. NMR (D20) 1H: 6 2.88 (4H, m), 1.6 (4H, m). 31P: 6 = -2.98, -3.44 s. Anal.: Calcd. : C, 22.64; H, 3.14. Found: C, 22.41; H, 3.2.

Pisodium dihydrogen suberoylbisphosphonate, yield 90%, m.p.>250e. IR (KBr) 1677s, 1214s, 1110s, 1075s cm-1. N R (D20) ½: 6 2.8 (4H, t, J=7.2 Hz), 1.58 (4H, m), 1.31 (4H, m), 31P: δ = -3.23, 3.7s. Anal.: Calcd. C, 27.75; H, 4.05, Found, C, 26.93; H, 3.92.

Disodium dihydrogen terephthaloylbisphosphonate, yield 90%, m.p.>2506C, IR: 1641, 1682 cm"1.

Disodium dihydrogen isophthaloylbisphosphonate, yield 90%, m.p.>250°C, IR: 1641 cm"1.

Synthesis of MHO3P-CO-(CH2)10-CO-PO3HM (M = Cation, e.g. Na+, Li+, etc. ) Dihydrogen disodium dodecanedioylphosphonate. Tetramethyl dodecanedioyldiphosphonate obtained in the previous step was dissolved in dry benzene (30 ml) and cooled to 0°C. 30.2 g (0.198 mole) of bromotrimethylsilane was added slowly to the solution and the reaction mixture was stirred at the ambient temperature for one hour. The reaction mixture was evaporated in vacum (keeping the temperature below 30° ) and a solition of sodium hydroxide 2.4 g (0.06 mol) in methanol (70 ml ) was added with stirring to the residue and the reaction mixture was stirred for 2 hrs. at ambient temperature and the white precipitate was filtered, washed with methanol (15 ml) 31 and dried. In the P nmr spectrum the product showed a 1 singlet at 6 -0.2 ppm. H nmr spectrum 6 2.50 ppm (4H, t), 1.24 ppm (4H, m), 0.99 ppm (12H, broad singlet).

Synthesis of dialkyl a, a' -bishydroxyiminobisphosphonate disalts. 0.01 mole of dimethyl dilithium α,α'-bishydroxyiminobisphosphonate was suspended in absolute ethanol (10 ml), in flask A. In a separate flask 0.03 mol sodium was dissolved in absolute ethanol ( 10 ml ) , in an ice bath under a reflux condenser, equipped with a calcium chloride tube. The resulting solution was added slowly to a solution of 0.03 mol hydroxylamine hydrochloride in methanol (15 ml), until the solution was neutral to pH paper. After stirring for 5 minutes in an ice bath, sodium chloride was filtered, washed with ethanol and the filtrate was added to the solution of dimethyl dilithium salt in flask A. The reaction mixture was left to stir for 1-2 days at the ambient temperature, it was filtered, washed successively with acetonitrile and ether and dried in vacuo at room temperature .

Dimethyl dilithium 1 , 6-bishydroxyiminohexmethylene-l , 6-bisphosphonate was obtained in a yield of 90%, m.p.>250°C, IR (KBr): 1650w,b, 1221s, 1085s, 1049s cm-1. NMR (D20) ½: δ 3.55 (6H, d, J=10.8 Hz), 2.5 (4H, m), 1.62 (4H, m).

Dimethyl dilithium 1 , 8-bishydroxyimlnooctamethylene-l , 8-bisphosphonate yield 90%, m.p.>250°C, IR (KBr): 1665w,b, 1227s, 1087s, 1050s cm-1. NMR (D20): 1H 6 3.56 (6H, d, J=10.89 Hz), 2.5 (4H, m), 1.6 (4H, m), 1.4 (4H, m). a Octamethyl 1, 12-dihydroxydodecane-l , 1, 12, 12-tetrakisphosphonate 5.34 g (0.02 mol) 1 , 12-dodecanedioyl dichloride was added dropwise, with strirring, in a nitrogen atmosphere, to a mixture of 4.96 g (0.04 mol) of trimethyl phosphite and 4.40 g ( 0.04 mol ) of dimethyl phosphite at room temperature . The mixture was stirred for 10 hrs at 906C and the reaction mixture was evaporated under reduced pressure. The oily residue (9.8 g) showed a broad signal at 6=22.3 ppm in the nmr spectrum. 1.12-Dihydroxydodecane-l , 1 , 12, 12-tetrakisphosphonic acid tetrasodium salt. 0 0 II II [NaH0 Pi C — (CH ) C EP 0 HNa] 2 2 I 2 10 I 2 OH OH The product obtained in the previous step was dissolved in concentrated (32%) hydrochloric acid and refluxed for 72 hrs. The solution was cooled and filtered to remove impurities, the excess acid was evaporated, the rediue dissolved in warm distilled water and concentrated solution of sodium hydroxide was added dropwise, to pH 4 when the product started to precipitate. The precipitate which was collected by 31 filtration, showed in the P nmr spectrum a triplet at 6=19.58 ppm (J=14 Hz).

In vitro test: A novel bisphosphonat according to the present invention was added to a mixture of calcium chloride and sodium phosphate. After a period of time the calcium and phosphous concentration in the fitrate was determined.

Fig. 2 shows that adipoylbisphosphonate (C4 diacid) NaO ONa prevents the precipitation of calcium and phosphorus in the solution highly effectively while the CIO analog is only slightly effective.

The tetrakisphosphpnate is also highly effective. The above novel phosphonates were compared to two commercial compounds 0 CH0 0 li I 3 II /OH (NaEHDP) HO - P - C - P NaO I V ONa OH 0 CI 0 HO II I II OH and to (C1 DD) ^ P - C - P NaO I x0Na CI In vivo test: Fig. 2 shows in vivo anticalcification effect of novel bisphosphonates .

The novel bisphosphonates were: 0 0 0 0 HO J! II II I) - C - ( CH ) - C - P - OH NaO 6 \ ONa 0 0 0 0 HO |l II ... II ■ II - C - (CH„ ) - C - P - OH NaO 4 \ ONa They were compared to the commercial comounds: H: C12MDP and to NaEHDP.

Claims (8)

1. Bisphosphonates of the formula wherein m and f are independently 1 or 2 represents a hydrogen, or lower alkyl group, or an alkali metal cation; 1¾2 represents a hydrogen, a lower alkyl group or an alkali metal cation; Y represents =0 or =N-0H, or -OH; X represents -(CH2)n- or a branched alkylene group or a branched or straight alkenylene or alkynylene chain optionally substituted by one or more oxygen or nitrogen atoms ; wherein n is an integer from 3 to 24 with the provision that when Y is =0 and R1 and R2 denote a lower alkyl group, n represents an integer from 9 to 20; and with the provision that when £=m=2 and Y is -OH and and R2 are methyl groups, n represents an integer from 9-24; and with further provision that when l=m-

2. Y is an-OH group and R1=R2=hydrogen, n represents 3,5, and an integer from 7-24. or X is -(CH2) - (OCH2CH2) - 0 - (CH2) - P q P' wherein p and p' are independently integers from 1 to 5 q is an integer from 1 to 6 or X is -(CH ) - 0 - (CH„) - 0 - (CH ) - Δ t Δ s Δ t' wherein t and t' are independently integers from 1 to 6 s is an integer from 2 to 12 or X is - B - A - B wherein B represents a branched group or straight alkylene, an alkenylene or alkynylene chain optionally substituted by oxygen or nitrogen. A represents an aromatic groups selected from phenylene, naphthalenediyl, thiophenediyl or furandiyl . A compound according to claim 1 wherein Rx is hydrogen or a methyl group.

3. A compound according to claim 1 wherein 1*2 is a methyl group or sodium or lithium cation.

4. A compound according to claim 1 wherein n is 4 or 6.

5. A compound according to claim 1 wherein B is -CH2~ group.

6. A compound according to claim 1 wherein A is phenylene.

7. Tetramethyl adipoylbisphosphonate, according to claim 1.

8. Tetramethyl suberoylbisphosphonate, according to claim 1.

9. Dimethyl dilithium adipoylbisphosphonate, according to claim 1.

10. Disodium dihydrogen adipoylbisphosphonate, according to claim 1.

11. Disodium dihydrogen suberoylbisphosphonate, according to claim 1.

12. Disodium dihydrogen isophthaloylbisphosphonate, according to claim 1.

13. Disodium dihydrogen isophthaloylbisphosphonates, according to claim 1.

14. Dimethyl dilithium 1, 6-bishydroxyiminohexamethylene-l, 6- bisphosphonate, according to claim 1.

15. Dimethyl dilithium 1, 8-bishydroxyiminooctamethylene-l, 8- bisphosphonate, according to claim 1.

16. Octamethyl 1, 12-dihydroxydodecane-l, 1, 12, 12- tetrakisphosphonate, according to claim 1.

17. 1, 12-dihydroxydodecane-l, 1, 12, 12-tetrakisphosphonic acid tetra sodium salt, according to claim 1.

18. Tetramethyl dodecanedioyldiphosphonate, according to claim 1.

19. Dihydrogen disodium. dodecanedioyldiphosphonate, according to claim 1.

20. A process for producing tetraalkyl α, '- diketobisphosphonates according to claim 1 which process comprises reacting trialkyl phosphite with dicarboxylic acid dihalide.

21. A process for producing dialkyl a, a' -diketobisphosphonate disalts according to claim 1 which process comprises reacting tetraalkyl diketobisphosphonate with sodium iodide or lithium bromide.

22. A process for producing dihydrogen ,α'- diketobisphosphonate disalts according to claim 1 wherein tetraalkyl diketobisphosphonate is dissolved in dry aprotic solvents and bromotrimethylsilane is added slowly, and a solution of sodium hydoxide is added after the evaporation of the solvent.

23. A process for producing dialkyl α, '- bishydroxyiminobisphosphonate disalts according to claim 1 wherein dialkyl dilithium α,α'- bishydroxyiminobisphosphonate is suspended in absolute ethanol and in separate flask sodium is suspended in absolute ethanol and the resulting solution is added slowly to hydroxylamine in methanol.

24. A process for producing octaalkyl aw dihydroxy aa ww tetrakisphosphonates by adding aw dicarboxylic acid dihalide to a mixture of trialkyl [phosphite and dialkylphosphite .

25. A process for producing aw dihydroxy aa WW tetrakisphosphonic acid by acid hydrolysis of the corresponding octaalkyl esters.

26. A process for obtaining the compounds as defined in claim 1 in accordance with the examples.

27. Pharmaceutical compositions which comprise a compound according to claim 1 as active ingredient, and suitable carriers optionally suitable for controlled release delivery systems and/or other additives. 28 « Pharmaceutical compositions according to claim 27 n which the carrier is suitable for a controlled release delivery system. 29 Pharmaceutical compositions according to claim 28 wherein the carrier is based on a polymeric vehicle. Pharmaceutical compositions according to claim 29 wherein said polymeric vehicle is based on silicon or polyurethane or any other biocompatible polymer. Pharmaceutical compositions according to claim 28 wherein the carrier is based on a degradable system. Pharmaceutical compositions according to claim 31 wherein said degradable carrier is based on Chitosan or collagen or any other degradable/biodegradable carrier. Pharmaceutical compositions according to claim 28 wherein the controlled release delivery system is utilized by subdermal implantation. Pharmaceutical compositions according to claim 28 wherein the controlled release delivery system is utilized by site specific implantation. Pharmaceutical compositions according to the preceding claims for use in the treatment of Osteoporosis (including disuse and postmenopausal osteoporosis); Hypercalcemia of Malignancy; Heterotopic Ossification (Hip arthroplasty, Spinal cord injury, Myositis ossificans); Paget' s disease; Hyperphosphatemia (e.g. Diabetes); (Direct) anticancer effect.

36. Pharmaceutical compositions according to the preceding claims useful as diagnostics.

37. Compounds according to claim 1 useful as Adhesives; Agents for extraction, concentration, and purification of uranium, thorium, and plutonium; Antioxidants; Antistatic agents; Blowing agents; Catalysts; Corrosion inhibitors; Coupling agents; Crystallization inhibitors; Dentifrice compositions; Deodorants; Detergent additives; Detergents for cleaning metal surfaces; Dye modifiers; Flame retardant polymers; Flame retardants for textiles; Fire retardants for synthetic fibers; Flotation agents; Fuel additives; Gelling agents; Hardening oil composites; Heat and light stablizers; Hydraulic fluid additives; Ion exchange resins; Lubricants; Photography; Plasticizers; Polyester, polyethylene, and polycarbonate discoloration inhibitors; Polyurethan additives; Rayon additives; Resin and plastic additives; Scale inhibitors; Settling retardants; Sequestering agents; Solvent extraction; Suspending agents; Synthetic fiber preparation; Viscosity modifiers; Wood fireproofing agents. Or. MEIR NOAM ADVOCATE 8. PATENT ATTORNEY