IL92688A - A gene and structure of a gene that encodes an aminotransparasomicroorganism that expresses this gene - Google Patents

A gene and structure of a gene that encodes an aminotransparasomicroorganism that expresses this gene

Info

Publication number: IL92688A
Authority: IL; Israel
Prior art keywords: gene; transaminase; ppt; ala; coli
Prior art date: 1988-12-15

Application number

IL9268889A

Other languages

English (en)

Hebrew (he)

Other versions

IL92688A0 (en

Original Assignee

Hoechst Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-12-15

Filing date

1989-12-13

Publication date

1996-10-31

1988-12-15 Priority claimed from DE3842174A external-priority patent/DE3842174A1/de

1989-12-13 Application filed by Hoechst Ag filed Critical Hoechst Ag

1990-09-17 Publication of IL92688A0 publication Critical patent/IL92688A0/xx

1996-10-31 Publication of IL92688A publication Critical patent/IL92688A/en

Links

108090000340 Transaminases Proteins 0.000 title claims description 66
108090000623 proteins and genes Proteins 0.000 title claims description 65
102000003929 Transaminases Human genes 0.000 title claims description 54
244000005700 microbiome Species 0.000 title claims description 10
230000000694 effects Effects 0.000 claims description 38
239000012634 fragment Substances 0.000 claims description 34
239000013612 plasmid Substances 0.000 claims description 27
102000004190 Enzymes Human genes 0.000 claims description 21
108090000790 Enzymes Proteins 0.000 claims description 21
150000001413 amino acids Chemical group 0.000 claims description 21
241000588724 Escherichia coli Species 0.000 claims description 15
241001452028 Escherichia coli DH1 Species 0.000 claims description 13
238000000034 method Methods 0.000 claims description 11
101100173636 Rattus norvegicus Fhl2 gene Proteins 0.000 claims description 9
238000012217 deletion Methods 0.000 claims description 8
230000037430 deletion Effects 0.000 claims description 8
NIBLDNQQTIFBAI-BYPYZUCNSA-N CPCC[C@H](N)C(O)=O Chemical compound CPCC[C@H](N)C(O)=O NIBLDNQQTIFBAI-BYPYZUCNSA-N 0.000 claims description 6
241001646716 Escherichia coli K-12 Species 0.000 claims description 6
238000004519 manufacturing process Methods 0.000 claims description 6
238000007792 addition Methods 0.000 claims description 5
238000005891 transamination reaction Methods 0.000 claims description 5
108091028043 Nucleic acid sequence Proteins 0.000 claims description 3
YJTNHDYMQPHXFO-UHFFFAOYSA-N 4-(hydroxymethylphosphinyl)-2-oxobutyric acid Chemical compound CP(O)(=O)CCC(=O)C(O)=O YJTNHDYMQPHXFO-UHFFFAOYSA-N 0.000 claims description 2
230000000707 stereoselective effect Effects 0.000 claims description 2
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 17
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BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 7
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101100508919 Arabidopsis thaliana IPT6 gene Proteins 0.000 description 4
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JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
108010052305 exodeoxyribonuclease III Proteins 0.000 description 4
BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 4
239000002773 nucleotide Substances 0.000 description 4
125000003729 nucleotide group Chemical group 0.000 description 4
YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 4
239000000523 sample Substances 0.000 description 4
229910000162 sodium phosphate Inorganic materials 0.000 description 4
239000001488 sodium phosphate Substances 0.000 description 4
239000006228 supernatant Substances 0.000 description 4
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 4
241000894006 Bacteria Species 0.000 description 3
238000003556 assay Methods 0.000 description 3
238000010367 cloning Methods 0.000 description 3
229960003692 gamma aminobutyric acid Drugs 0.000 description 3
239000001963 growth medium Substances 0.000 description 3
108010085325 histidylproline Proteins 0.000 description 3
238000000338 in vitro Methods 0.000 description 3
238000010369 molecular cloning Methods 0.000 description 3
150000008300 phosphoramidites Chemical class 0.000 description 3
238000012163 sequencing technique Methods 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
108091033380 Coding strand Proteins 0.000 description 2
108020004705 Codon Proteins 0.000 description 2
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 description 2
239000002253 acid Substances 0.000 description 2
108010076324 alanyl-glycyl-glycine Proteins 0.000 description 2
108010077245 asparaginyl-proline Proteins 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
238000010276 construction Methods 0.000 description 2
239000013601 cosmid vector Substances 0.000 description 2
239000000287 crude extract Substances 0.000 description 2
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238000009396 hybridization Methods 0.000 description 2
230000001965 increasing effect Effects 0.000 description 2
238000003780 insertion Methods 0.000 description 2
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238000002955 isolation Methods 0.000 description 2
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108020004707 nucleic acids Proteins 0.000 description 2
102000039446 nucleic acids Human genes 0.000 description 2
150000007523 nucleic acids Chemical class 0.000 description 2
230000003287 optical effect Effects 0.000 description 2
238000003752 polymerase chain reaction Methods 0.000 description 2
NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 2
238000011160 research Methods 0.000 description 2
108010061238 threonyl-glycine Proteins 0.000 description 2
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
PPINMSZPTPRQQB-NHCYSSNCSA-N 2-[[(2s)-1-[(2s)-2-[[(2s)-2-amino-3-methylbutanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]acetic acid Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)NCC(O)=O PPINMSZPTPRQQB-NHCYSSNCSA-N 0.000 description 1
OTEWWRBKGONZBW-UHFFFAOYSA-N 2-[[2-[[2-[(2-azaniumylacetyl)amino]-4-methylpentanoyl]amino]acetyl]amino]acetate Chemical compound NCC(=O)NC(CC(C)C)C(=O)NCC(=O)NCC(O)=O OTEWWRBKGONZBW-UHFFFAOYSA-N 0.000 description 1
-1 3-carboxy-3-oxopropyl Chemical group 0.000 description 1
JBVSSSZFNTXJDX-YTLHQDLWSA-N Ala-Ala-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](C)N JBVSSSZFNTXJDX-YTLHQDLWSA-N 0.000 description 1
SSSROGPPPVTHLX-FXQIFTODSA-N Ala-Arg-Asp Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(O)=O SSSROGPPPVTHLX-FXQIFTODSA-N 0.000 description 1
XAEWTDMGFGHWFK-IMJSIDKUSA-N Ala-Asp Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CC(O)=O XAEWTDMGFGHWFK-IMJSIDKUSA-N 0.000 description 1
MIPWEZAIMPYQST-FXQIFTODSA-N Ala-Cys-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(O)=O MIPWEZAIMPYQST-FXQIFTODSA-N 0.000 description 1
VGPWRRFOPXVGOH-BYPYZUCNSA-N Ala-Gly-Gly Chemical compound C[C@H](N)C(=O)NCC(=O)NCC(O)=O VGPWRRFOPXVGOH-BYPYZUCNSA-N 0.000 description 1
NOGFDULFCFXBHB-CIUDSAMLSA-N Ala-Leu-Cys Chemical compound C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)O)N NOGFDULFCFXBHB-CIUDSAMLSA-N 0.000 description 1
CCDFBRZVTDDJNM-GUBZILKMSA-N Ala-Leu-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O CCDFBRZVTDDJNM-GUBZILKMSA-N 0.000 description 1
MEFILNJXAVSUTO-JXUBOQSCSA-N Ala-Leu-Thr Chemical compound C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O MEFILNJXAVSUTO-JXUBOQSCSA-N 0.000 description 1
PMQXMXAASGFUDX-SRVKXCTJSA-N Ala-Lys-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@H](C)N)CCCCN PMQXMXAASGFUDX-SRVKXCTJSA-N 0.000 description 1
YCRAFFCYWOUEOF-DLOVCJGASA-N Ala-Phe-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)C)CC1=CC=CC=C1 YCRAFFCYWOUEOF-DLOVCJGASA-N 0.000 description 1
WPWUFUBLGADILS-WDSKDSINSA-N Ala-Pro Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(O)=O WPWUFUBLGADILS-WDSKDSINSA-N 0.000 description 1
FQNILRVJOJBFFC-FXQIFTODSA-N Ala-Pro-Asp Chemical compound C[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)O)C(=O)O)N FQNILRVJOJBFFC-FXQIFTODSA-N 0.000 description 1
IPWKGIFRRBGCJO-IMJSIDKUSA-N Ala-Ser Chemical compound C[C@H]([NH3+])C(=O)N[C@@H](CO)C([O-])=O IPWKGIFRRBGCJO-IMJSIDKUSA-N 0.000 description 1
NCQMBSJGJMYKCK-ZLUOBGJFSA-N Ala-Ser-Ser Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O NCQMBSJGJMYKCK-ZLUOBGJFSA-N 0.000 description 1
VYMJAWXRWHJIMS-LKTVYLICSA-N Ala-Tyr-His Chemical compound C[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CC2=CN=CN2)C(=O)O)N VYMJAWXRWHJIMS-LKTVYLICSA-N 0.000 description 1
UISQLSIBJKEJSS-GUBZILKMSA-N Arg-Arg-Ser Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CO)C(O)=O UISQLSIBJKEJSS-GUBZILKMSA-N 0.000 description 1
OQCWXQJLCDPRHV-UWVGGRQHSA-N Arg-Gly-Leu Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC(C)C)C(O)=O OQCWXQJLCDPRHV-UWVGGRQHSA-N 0.000 description 1
NKNILFJYKKHBKE-WPRPVWTQSA-N Arg-Gly-Val Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](C(C)C)C(O)=O NKNILFJYKKHBKE-WPRPVWTQSA-N 0.000 description 1
KSHJMDSNSKDJPU-QTKMDUPCSA-N Arg-Thr-His Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 KSHJMDSNSKDJPU-QTKMDUPCSA-N 0.000 description 1
UTSMXMABBPFVJP-SZMVWBNQSA-N Arg-Val-Trp Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N UTSMXMABBPFVJP-SZMVWBNQSA-N 0.000 description 1
QEYJFBMTSMLPKZ-ZKWXMUAHSA-N Asn-Ala-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(O)=O QEYJFBMTSMLPKZ-ZKWXMUAHSA-N 0.000 description 1
FBODFHMLALOPHP-GUBZILKMSA-N Asn-Lys-Glu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(O)=O FBODFHMLALOPHP-GUBZILKMSA-N 0.000 description 1
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REQUGIWGOGSOEZ-ZLUOBGJFSA-N Asn-Ser-Asn Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)N)C(=O)O)N)C(=O)N REQUGIWGOGSOEZ-ZLUOBGJFSA-N 0.000 description 1
OERMIMJQPQUIPK-FXQIFTODSA-N Asp-Arg-Ala Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(O)=O OERMIMJQPQUIPK-FXQIFTODSA-N 0.000 description 1
NAPNAGZWHQHZLG-ZLUOBGJFSA-N Asp-Asp-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(=O)O)N NAPNAGZWHQHZLG-ZLUOBGJFSA-N 0.000 description 1
JOCQXVJCTCEFAZ-CIUDSAMLSA-N Asp-His-Asn Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(N)=O)C(O)=O JOCQXVJCTCEFAZ-CIUDSAMLSA-N 0.000 description 1
AYFVRYXNDHBECD-YUMQZZPRSA-N Asp-Leu-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O AYFVRYXNDHBECD-YUMQZZPRSA-N 0.000 description 1
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FYYSIASRLDJUNP-WHFBIAKZSA-N Glu-Asp Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(O)=O FYYSIASRLDJUNP-WHFBIAKZSA-N 0.000 description 1
DSPQRJXOIXHOHK-WDSKDSINSA-N Glu-Asp-Gly Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(O)=O DSPQRJXOIXHOHK-WDSKDSINSA-N 0.000 description 1
WNRZUESNGGDCJX-JYJNAYRXSA-N Glu-Leu-Phe Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O WNRZUESNGGDCJX-JYJNAYRXSA-N 0.000 description 1
YGLCLCMAYUYZSG-AVGNSLFASA-N Glu-Lys-His Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 YGLCLCMAYUYZSG-AVGNSLFASA-N 0.000 description 1
NNQDRRUXFJYCCJ-NHCYSSNCSA-N Glu-Pro-Val Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(O)=O NNQDRRUXFJYCCJ-NHCYSSNCSA-N 0.000 description 1
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230000006862 enzymatic digestion Effects 0.000 description 1
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239000012530 fluid Substances 0.000 description 1
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108010089804 glycyl-threonine Proteins 0.000 description 1
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101150056694 hisC gene Proteins 0.000 description 1
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230000001939 inductive effect Effects 0.000 description 1
108010044056 leucyl-phenylalanine Proteins 0.000 description 1
108010000761 leucylarginine Proteins 0.000 description 1
108010091871 leucylmethionine Proteins 0.000 description 1
230000004807 localization Effects 0.000 description 1
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238000002360 preparation method Methods 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Chemical Kinetics & Catalysis (AREA)
Medicinal Chemistry (AREA)
General Chemical & Material Sciences (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

IL9268889A 1988-12-15 1989-12-13 A gene and structure of a gene that encodes an aminotransparasomicroorganism that expresses this gene IL92688A (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE3842174A DE3842174A1 (de)	1988-12-15	1988-12-15	Gen und genstruktur, codierend fuer eine aminotransferase, und mikroorganismen, die dieses gen exprimieren
DE3932015A DE3932015A1 (de)	1988-12-15	1989-09-26	Gen und genstruktur, codierend fuer eine aminotransferase, mikroorganismen, die dieses gen exprimieren, und transaminierungsverfahren unter verwendung des expressionsprodukts

Publications (2)

Publication Number	Publication Date
IL92688A0 IL92688A0 (en)	1990-09-17
IL92688A true IL92688A (en)	1996-10-31

Family

ID=25875159

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL9268889A IL92688A (en)	1988-12-15	1989-12-13	A gene and structure of a gene that encodes an aminotransparasomicroorganism that expresses this gene

Country Status (10)

Country	Link
US (4)	US5221737A (xx)
EP (1)	EP0373597B1 (xx)
JP (1)	JP2986492B2 (xx)
KR (1)	KR0159764B1 (xx)
AU (1)	AU626357B2 (xx)
DE (2)	DE3932015A1 (xx)
DK (1)	DK634289A (xx)
ES (1)	ES2059691T3 (xx)
IL (1)	IL92688A (xx)
NZ (1)	NZ231748A (xx)

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* Cited by examiner, † Cited by third party
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DE3818851A1 (de) *	1988-06-03	1989-12-14	Hoechst Ag	Neue transaminase, ihre herstellung und ihre verwendung
DE3932015A1 (de) *	1988-12-15	1991-04-04	Hoechst Ag	Gen und genstruktur, codierend fuer eine aminotransferase, mikroorganismen, die dieses gen exprimieren, und transaminierungsverfahren unter verwendung des expressionsprodukts
US6455287B1 (en)	1995-02-23	2002-09-24	Wyeth	Mechanical disruption of bacterial cells for plasmid recovery
DE19919848A1 (de)	1999-04-30	2000-11-02	Aventis Cropscience Gmbh	Verfahren zur Herstellung von L-Phosphinothricin durch enzymatische Transaminierung mit Aspartat
US8498207B2 (en) *	2008-06-26	2013-07-30	Reverb Networks	Dynamic load balancing
US20110090820A1 (en)	2009-10-16	2011-04-21	Osama Hussein	Self-optimizing wireless network
US9826416B2 (en) *	2009-10-16	2017-11-21	Viavi Solutions, Inc.	Self-optimizing wireless network
US8385900B2 (en) *	2009-12-09	2013-02-26	Reverb Networks	Self-optimizing networks for fixed wireless access
US8509762B2 (en)	2011-05-20	2013-08-13	ReVerb Networks, Inc.	Methods and apparatus for underperforming cell detection and recovery in a wireless network
US9369886B2 (en)	2011-09-09	2016-06-14	Viavi Solutions Inc.	Methods and apparatus for implementing a self optimizing-organizing network manager
US9258719B2 (en)	2011-11-08	2016-02-09	Viavi Solutions Inc.	Methods and apparatus for partitioning wireless network cells into time-based clusters
CN106399458A (zh) *	2011-11-10	2017-02-15	福满代谢组技术有限公司	乙醇胺磷酸酯的测定方法
US9008722B2 (en)	2012-02-17	2015-04-14	ReVerb Networks, Inc.	Methods and apparatus for coordination in multi-mode networks
US9113353B1 (en)	2015-02-27	2015-08-18	ReVerb Networks, Inc.	Methods and apparatus for improving coverage and capacity in a wireless network
US9834802B2 (en)	2016-03-02	2017-12-05	Agrimetis, Llc	Methods for making L-glufosinate
CN107119084B (zh) *	2017-03-23	2019-12-17	浙江大学	一种利用转氨酶和乙烯合成酶生产l-草铵膦的方法
CN107630052B (zh) *	2017-03-29	2018-05-29	武汉茵茂特生物技术有限公司	L-草铵膦的生物转化方法
CN110343676B (zh)	2018-04-03	2020-06-23	上海弈柯莱生物医药科技有限公司	一种l-谷氨酸脱氢酶突变体及其应用
CN111019916B (zh)	2018-11-23	2020-12-08	弈柯莱生物科技(上海)股份有限公司	一种d-氨基酸氧化酶突变体及其应用
CN111979208B (zh)	2019-05-23	2023-01-10	弈柯莱生物科技(上海)股份有限公司	一种l-谷氨酸脱氢酶突变体及其应用

Family Cites Families (8)

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Publication number	Priority date	Publication date	Assignee	Title
JPS6178378A (ja) *	1984-09-27	1986-04-21	Ajinomoto Co Inc	芳香族アミノ酸生合成遺伝子を含有する組換えｄｎａ及びそれを有するコリネ型細菌
DE3576523D1 (de) *	1984-11-20	1990-04-19	Ajinomoto Kk	Rekombinante dna enthaltende coryneformbakterien und verfahren zur herstellung von aromatischen aminosaeuren unter verwendung dieser bakterien.
ES2144999T3 (es) *	1986-06-04	2000-07-01	Hoechst Schering Agrevo Gmbh	Procedimiento para la preparacion de l-leucina terciaria mediante transaminacion.
US4968629A (en) *	1986-08-20	1990-11-06	Hematology Marketing Associates, Inc.	Blood diluent for automatic and semi-automatic determination of white cells and method of utilizing same
DE3636722A1 (de) *	1986-10-29	1988-05-05	Hoechst Ag	Klonierung und verwendung des transaminase-gens ilve
DE3818851A1 (de) *	1988-06-03	1989-12-14	Hoechst Ag	Neue transaminase, ihre herstellung und ihre verwendung
EP0349965A3 (en) *	1988-07-04	1990-06-13	Meiji Seika Kaisha Ltd.	Novel genes encoding transaminase
DE3932015A1 (de) *	1988-12-15	1991-04-04	Hoechst Ag	Gen und genstruktur, codierend fuer eine aminotransferase, mikroorganismen, die dieses gen exprimieren, und transaminierungsverfahren unter verwendung des expressionsprodukts

1989
- 1989-09-26 DE DE3932015A patent/DE3932015A1/de not_active Withdrawn
- 1989-12-12 ES ES89122939T patent/ES2059691T3/es not_active Expired - Lifetime
- 1989-12-12 DE DE89122939T patent/DE58905773D1/de not_active Expired - Fee Related
- 1989-12-12 EP EP89122939A patent/EP0373597B1/de not_active Expired - Lifetime
- 1989-12-13 NZ NZ231748A patent/NZ231748A/xx unknown
- 1989-12-13 US US07/450,230 patent/US5221737A/en not_active Expired - Lifetime
- 1989-12-13 KR KR1019890018445A patent/KR0159764B1/ko not_active Expired - Fee Related
- 1989-12-13 IL IL9268889A patent/IL92688A/en not_active IP Right Cessation
- 1989-12-14 AU AU46146/89A patent/AU626357B2/en not_active Ceased
- 1989-12-14 DK DK634289A patent/DK634289A/da not_active Application Discontinuation
- 1989-12-15 JP JP1325770A patent/JP2986492B2/ja not_active Expired - Fee Related
1994
- 1994-11-04 US US08/337,453 patent/US5888782A/en not_active Expired - Fee Related
1995
- 1995-06-07 US US08/488,000 patent/US6210934B1/en not_active Expired - Fee Related
1997
- 1997-11-26 US US08/978,817 patent/US6051408A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
EP0373597B1 (de)	1993-09-29
EP0373597A3 (de)	1991-08-28
US6051408A (en)	2000-04-18
DE58905773D1 (de)	1993-11-04
US5888782A (en)	1999-03-30
KR900009981A (ko)	1990-07-06
DE3932015A1 (de)	1991-04-04
JPH02207789A (ja)	1990-08-17
IL92688A0 (en)	1990-09-17
US6210934B1 (en)	2001-04-03
NZ231748A (en)	1991-10-25
KR0159764B1 (ko)	1998-11-16
DK634289D0 (da)	1989-12-14
JP2986492B2 (ja)	1999-12-06
AU626357B2 (en)	1992-07-30
EP0373597A2 (de)	1990-06-20
US5221737A (en)	1993-06-22
AU4614689A (en)	1990-06-21
DK634289A (da)	1990-06-16
ES2059691T3 (es)	1994-11-16

Publication	Publication Date	Title
IL92688A (en)	1996-10-31	A gene and structure of a gene that encodes an aminotransparasomicroorganism that expresses this gene
Deutch et al.	1982	Escherichia coli Δ 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification
Chung et al.	1988	Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map
Kuliopulos et al.	1987	Isolation and sequencing of the gene encoding delta 5-3-ketosteroid isomerase of Pseudomonas testosteroni: overexpression of the protein.
CA2246243A1 (en)	1997-08-14	Transaminases and aminotransferases
WO1991002801A1 (en)	1991-03-07	An in vitro packaging system for cloning dna fragments of 95 kb
Paluh et al.	1985	Study of anthranilate synthase function by replacement of cysteine 84 using site-directed mutagenesis.
KR960015264B1 (ko)	1996-11-07	데아세톡시세팔로스포린 c합성효소 및 데아세틸 세팔로스포린 c합성효소를 암호화하는 재조합 dna 발현 벡터 및 dna 화합물
Bartsch et al.	1990	Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: cloning, characterization, and overexpression of the gene encoding a phosphinothricin-specific transaminase from Escherichia coli
JP2754975B2 (ja)	1998-05-20	ファルネシル二リン酸合成酵素およびそれをコードするｄｎａ配列
EP0329710A1 (en)	1989-08-30	Microbial production of peptide oligomers
JP3325597B2 (ja)	2002-09-17	ポリペプチドの製法
JP3489604B2 (ja)	2004-01-26	５−アミノレブリン酸合成酵素遺伝子
AU659237B2 (en)	1995-05-11	Gene coding for esterase and novel microorganism containing said gene
David et al.	1991	Citrate synthase from Mycobacterium smegmatis. Cloning, sequence determination and expression in Escherichia coli
Said et al.	1988	Nucleotide sequencing and expression of the fadL gene involved in long‐chain fatty acid transport in Escherichia coli
FI74732C (fi)	1988-03-10	Dna-oeverfoeringsvektor, vilken innehaoller en nukleotidsekvens som kodar foer tillvaexthormon.
JP2003189863A (ja)	2003-07-08	アスパラギン酸アミド並びにその誘導体の製造方法
FI65447C (fi)	1984-05-10	Foerfarande foer framstaellning av en bakterie som innehaolleren foer tillvaexthormon kodande nukleotidsekvens
WO1994016063A1 (en)	1994-07-21	Periplasmic 3':5'-cyclic nucleotide phosphodiesterase and related methods for producing and using the same
KR970001566B1 (ko)	1997-02-11	티로신 페놀리아제(Tyrosine phenollyase)를 코드(code)하는 유전자, 상기 유전자에 의해 형질전환된 대장균 및 이를 이용한 티로신 페놀리아제의 제조방법
JPH10262674A (ja)	1998-10-06	アルカリホスファターゼをコードする遺伝子
JPH05317055A (ja)	1993-12-03	新規なウリカーゼ遺伝子、新規な組み換え体ｄｎａ及びウリカーゼの製造法
CA1337552C (en)	1995-11-14	In-vitro headful packaging system for cloning dna fragments as large as 95 kb
JPH01500960A (ja)	1989-04-06	ヒト白血球インターフェロンα‐Ｉ１の生合成をコードする組換えプラスミドＤＮＡ　ｐＶＮ２２及びそれを含むヒト白血球インターフェロンα‐Ｉ１の生産体であるシュードモナスＳＰ．３１（ｐＶＮ２２）菌株

Legal Events

Date	Code	Title
1997-03-18	FF	Patent granted
1997-09-30	KB	Patent renewed
2000-06-01	KB	Patent renewed
2004-02-08	KB	Patent renewed
2008-01-22	KB	Patent renewed
2010-11-30	EXP	Patent expired