IL84381A - תהליך לשינוי גנטי של צמחים חד-פסיגיים ממשפחת ה-גרמינאי )eaenimarg( ע" ישימוש ב-אגרובקטוריום )muiretcaborga( - Google Patents

תהליך לשינוי גנטי של צמחים חד-פסיגיים ממשפחת ה-גרמינאי )eaenimarg( ע" ישימוש ב-אגרובקטוריום )muiretcaborga(

Info

Publication number: IL84381A
Authority: IL; Israel
Prior art keywords: dna; process according; plants; plant; viral
Prior art date: 1986-11-07

Application number

IL8438187A

Other languages

English (en)

Other versions

IL84381A0 (en

Original Assignee

Ciba Geigy

Lubrizol Genetics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-11-07

Filing date

1987-11-05

Publication date

1994-08-26

1987-11-05 Application filed by Ciba Geigy, Lubrizol Genetics Inc filed Critical Ciba Geigy

1988-04-29 Publication of IL84381A0 publication Critical patent/IL84381A0/xx

1994-08-26 Publication of IL84381A publication Critical patent/IL84381A/he

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation

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Organic Chemistry (AREA)
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Bioinformatics & Cheminformatics (AREA)
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Plant Pathology (AREA)
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Cell Biology (AREA)
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Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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IL8438187A 1986-11-07 1987-11-05 תהליך לשינוי גנטי של צמחים חד-פסיגיים ממשפחת ה-גרמינאי )eaenimarg( ע" ישימוש ב-אגרובקטוריום )muiretcaborga( IL84381A (he)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
CH445686		1986-11-07
CH225587		1987-06-16

Publications (2)

Publication Number	Publication Date
IL84381A0 IL84381A0 (en)	1988-04-29
IL84381A true IL84381A (he)	1994-08-26

Family

ID=25689930

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL8438187A IL84381A (he)	1986-11-07	1987-11-05	תהליך לשינוי גנטי של צמחים חד-פסיגיים ממשפחת ה-גרמינאי )eaenimarg( ע" ישימוש ב-אגרובקטוריום )muiretcaborga(

Country Status (6)

Country	Link
EP (1)	EP0267159A3 (he)
AU (1)	AU611652B2 (he)
BR (1)	BR8705984A (he)
CA (1)	CA1340925C (he)
IL (1)	IL84381A (he)
NZ (1)	NZ222435A (he)

Families Citing this family (93)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5668298A (en) *	1984-12-24	1997-09-16	Eli Lilly And Company	Selectable marker for development of vectors and transformation systems in plants
CA1340766C (en) *	1984-12-24	1999-09-28	Clive Waldron	Selectable marker for development of vectors and transformation systems in plants
IL84459A (en) *	1986-12-05	1993-07-08	Agracetus	Apparatus and method for the injection of carrier particles carrying genetic material into living cells
ES2121803T3 (es) *	1987-05-20	1998-12-16	Novartis Ag	Plantas de zea mays y plantas transgenicas de zea mays generadas a partir de protoplastos o celulas derivadas de protoplastos.
JPH02503623A (ja) *	1987-05-22	1990-11-01	マックス‐プランク‐ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファウ	外来性遺伝情報の受容システムとしての有用植物の胚
EP0298918B1 (de) *	1987-07-10	2001-09-05	Syngenta Participations AG	Induzierbare Virusresistenz bei Pflanzen
CA1327173C (en) *	1987-07-21	1994-02-22	Erwin Heberle-Bors	Method of gene transfer into plants
WO1989005859A1 (en) *	1987-12-21	1989-06-29	The Upjohn Company	Agrobacterium mediated transformation of germinating plant seeds
JP2996995B2 (ja) *	1988-06-01	2000-01-11	ザテキサスエイアンドエムユニヴァーシティシステム	茎頂による植物の形質転換方法
BR9007159A (pt)	1989-02-24	1991-12-10	Monsanto Co	Genes sinteticos de plantas e processo para a preparacao dos mesmos
CA2017544A1 (en) *	1989-05-29	1990-11-29	Gunther Neuhaus	Process for the production of transgenic plants
US5550318A (en) *	1990-04-17	1996-08-27	Dekalb Genetics Corporation	Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6777589B1 (en)	1990-01-22	2004-08-17	Dekalb Genetics Corporation	Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6946587B1 (en) *	1990-01-22	2005-09-20	Dekalb Genetics Corporation	Method for preparing fertile transgenic corn plants
US6329574B1 (en)	1990-01-22	2001-12-11	Dekalb Genetics Corporation	High lysine fertile transgenic corn plants
JP3209744B2 (ja)	1990-01-22	2001-09-17	デカルブ・ジェネティクス・コーポレーション	結実能力のある遺伝子変換コーン
US5484956A (en) *	1990-01-22	1996-01-16	Dekalb Genetics Corporation	Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6395966B1 (en)	1990-08-09	2002-05-28	Dekalb Genetics Corp.	Fertile transgenic maize plants containing a gene encoding the pat protein
NL9002116A (nl) *	1990-09-27	1992-04-16	Clovis Matton N V	Werkwijze voor het genetisch manipuleren van plantecellen, recombinante plasmiden, recombinante bacterien, planten.
US5780709A (en) *	1993-08-25	1998-07-14	Dekalb Genetics Corporation	Transgenic maize with increased mannitol content
US6281411B1 (en)	1993-08-25	2001-08-28	Dekalb Genetics Corporation	Transgenic monocots plants with increased glycine-betaine content
US6326527B1 (en)	1993-08-25	2001-12-04	Dekalb Genetics Corporation	Method for altering the nutritional content of plant seed
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ES2164759T3 (es) *	1993-12-09	2002-03-01	Texas A & M Univ Sys	Transformacion de especies musa utilizando agrobacterium tumefaciens.
US6350934B1 (en)	1994-09-02	2002-02-26	Ribozyme Pharmaceuticals, Inc.	Nucleic acid encoding delta-9 desaturase
CN1208437A (zh)	1996-06-21	1999-02-17	孟山都公司	农杆菌介导转化法生产稳定转化的可育小麦的方法及其相关组分
US6183959B1 (en)	1997-07-03	2001-02-06	Ribozyme Pharmaceuticals, Inc.	Method for target site selection and discovery
AR022383A1 (es)	1998-09-18	2002-09-04	Univ Kentucky Res Found	Sintasas
CA2267014A1 (en) *	1999-03-26	2000-09-26	Brenda Rojas	Monocot transformation using acrobacterium
GB9923306D0 (en)	1999-10-01	1999-12-08	Isis Innovation	Diagnostic and therapeutic epitope, and transgenic plant
FR2815969B1 (fr)	2000-10-30	2004-12-10	Aventis Cropscience Sa	Plantes tolerantes aux herbicides par contournement de voie metabolique
CN1149918C (zh) *	2001-02-26	2004-05-19	山西省农业生物技术研究中心	农杆菌介导植物萌发种子基因转化方法
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ATE530656T1 (de)	2005-02-23	2011-11-15	Univ North Carolina State	Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
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US8319011B2 (en)	2006-12-15	2012-11-27	U.S. Smokeless Tobacco Company Llc	Tobacco plants having reduced nicotine demethylase activity
AU2008251445B2 (en)	2007-05-09	2014-04-03	Corteva Agriscience Llc	Novel herbicide resistance genes
CN101730712B (zh)	2007-06-08	2015-04-08	墨西哥国立大学	使用不需要钙黏着蛋白受体的毒素遏制昆虫对苏云金芽孢杆菌cry毒素的抗性
EP2573177B1 (en)	2007-11-12	2022-12-28	North Carolina State University	Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
ES2717102T3 (es)	2009-03-19	2019-06-19	Dsm Ip Assets Bv	Moléculas de ácido nucleico de ácido graso poliinsaturado sintasas, y polipéptidos, composiciones y métodos de preparación y usos de los mismos
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MX337123B (es)	2010-01-15	2016-02-12	Univ North Carolina State	Composiciones y metodos para minimizar la sintesis de nornicotina en el tabaco.
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WO2020051283A1 (en) *	2018-09-05	2020-03-12	The Regents Of The University Of California	Generation of heritably gene-edited plants without tissue culture
EP3898988A1 (en)	2018-12-20	2021-10-27	Benson Hill, Inc.	Pre-conditioning treatments to improve plant transformation
CA3187967A1 (en)	2020-06-24	2021-12-30	Benson Hill, Inc.	Plant cell treatments to improve plant transformation
CN114223426A (zh) *	2021-12-27	2022-03-25	中国热带农业科学院热带生物技术研究所	一种单子叶植物叶片液体注射方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3381568D1 (de) *	1983-01-13	1990-06-21	Max Planck Gesellschaft	Verfahren zum einbringen von expressionsfaehigen genen in pflanzenzellgenome und hybride ti plasmidvektoren enthaltende agrobacterium-staemme verwendbar in diesem verfahren.
WO1986003776A1 (en) *	1984-12-21	1986-07-03	Plant Genetic Systems N.V.	Process for preparing genetically stably transformed monocotyledonous plant cells
CA1341290C (en) *	1985-05-13	2001-09-11	Thomas Hohn	Method of genetically modifying plants
EP0288547A4 (en) *	1986-11-03	1991-03-20	Biotechnica International, Inc.	Plant transformation

1987
- 1987-11-02 EP EP87810628A patent/EP0267159A3/de not_active Withdrawn
- 1987-11-03 NZ NZ222435A patent/NZ222435A/en unknown
- 1987-11-05 CA CA000551094A patent/CA1340925C/en not_active Expired - Fee Related
- 1987-11-05 IL IL8438187A patent/IL84381A/he not_active IP Right Cessation
- 1987-11-06 BR BR8705984A patent/BR8705984A/pt not_active IP Right Cessation
- 1987-11-06 AU AU80893/87A patent/AU611652B2/en not_active Ceased

Also Published As

Publication number	Publication date
AU8089387A (en)	1988-05-12
AU611652B2 (en)	1991-06-20
IL84381A0 (en)	1988-04-29
EP0267159A3 (de)	1990-05-02
CA1340925C (en)	2000-03-14
BR8705984A (pt)	1988-06-14
EP0267159A2 (de)	1988-05-11
NZ222435A (en)	1990-04-26

Publication	Publication Date	Title
AU611652B2 (en)	1991-06-20	Process for the genetic modification of monocotyledonous plants
US5569597A (en)	1996-10-29	Methods of inserting viral DNA into plant material
US6376234B1 (en)	2002-04-23	Method of inserting viral DNA into plant material
Chabaud et al.	1988	Parameters affecting the frequency of kanamycin resistant alfalfa obtained by Agrobacterium tumefaciens mediated transformation
AU633248B2 (en)	1993-01-28	Agrobacterium mediated transformation of germinating plant seeds
US5668298A (en)	1997-09-16	Selectable marker for development of vectors and transformation systems in plants
AU620039B2 (en)	1992-02-13	Inducible virus resistance in plants
US20120192318A1 (en)	2012-07-26	Transformation system for Camelina sativa
JPWO1995006722A1 (ja)	1995-10-05	未熟胚の胚盤を用いた単子葉植物の形質転換方法
JPS60227683A (ja)	1985-11-12	単子葉類の植物のゲノム中に異質ｄｎａの組込みの為の方法
Yang et al.	2004	Large-scale production of enhancer trapping lines for rice functional genomics
WO1985004899A1 (en)	1985-11-07	Methods and vectors for transformation of plant cells
US6048730A (en)	2000-04-11	Selectable marker for development of vectors and transformation systems in plants
EP0205518A1 (en)	1986-12-30	Process for preparing genetically stably transformed monocotyledonous plant cells
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Legal Events

Date	Code	Title
1998-04-05	KB	Patent renewed
1998-11-26	HP	Change in proprietorship
2002-02-10	KB	Patent renewed
2006-02-05	KB	Patent renewed
2008-01-06	EXP	Patent expired